ライフサイエンスコーパス: filopodia

1 he formation of plasma membrane protrusions (filopodia).

2 educed the concentration of wild-type DAT in filopodia.

3 otes actin convergence to create the base of filopodia.

4 and spatiotemporal protein enrichment along filopodia.

5 and results in the formation of nonpolarized filopodia.

6 ntent of Arp2/3 and induced the formation of filopodia.

7 known to play a role in both invadopodia and filopodia.

8 not affect the number of substrate adherent filopodia.

9 h as formins elongating these filaments into filopodia.

10 Fascin is the main actin-bundling protein in filopodia.

11 inal release of highly procoagulant MPs from filopodia.

12 possible without noticeable movements of the filopodia.

13 surface projections such as lamellipodia and filopodia.

14 own-regulation promoted lateral movements of filopodia.

15 ADAPT can detect and morphologically profile filopodia.

16 [Ca(2+)] spikes colocalize with the bases of filopodia.

17 formation and stability of actin bundles in filopodia.

18 endogenous adhesion signaling to growth cone filopodia.

19 friction constants along the surface of the filopodia.

20 extend into the peripheral domain and enter filopodia.

21 tment of ArhGAP44 to actin-patches that seed filopodia.

22 nsity, F-actin content, and the formation of filopodia.

23 els of CP were detectable in the majority of filopodia.

24 lates the balance between dynamic and static filopodia.

25 he density of R60A, but not that of W63A, in filopodia.

26 nd close MT apposition to actin filaments in filopodia.

27 n filaments along axon shafts giving rise to filopodia.

28 nslocation of Arp2/3 and integrin-beta along filopodia.

29 turning from focal uncaging of Ca(2+) within filopodia.

30 of filopodia elongation to produce the long filopodia.

31 ated into a nanoscopic volume at the tips of filopodia.

32 actin filaments within the actin bundles of filopodia.

33 in extension direction, thus producing long filopodia.

34 ts, R60A and W63A, that do not accumulate in filopodia.

35 for its striking localization to the tips of filopodia.

36 state, also increased R60A concentration in filopodia.

37 nt targeting of DAT to, and accumulation in, filopodia.

38 in filaments and the formation of actin-rich filopodia.

39 platelets are more rigid and unable to form filopodia after stimulation.

40 Together, we show that filopodia allow the interpretation of the chemotactic gr

41 dynamic MTs from extending into growth cone filopodia along actin filament bundles.

42 NCAM2 activation increases the density of filopodia along neurites and neurite branching and outgr

43 xon arborization by affecting the density of filopodia along the shaft of the extending axon.

44 e, Heusermann et al. show that exosomes surf filopodia and are endocytosed in a process reminiscent t

45 Lack of SDF1alpha-induced filopodia and associated abnormal cell protrusions seen

46 is) in Fiji and R to measure fluorescence in filopodia and at their tips and bases concurrently with

47 of actin-filled membrane protrusions such as filopodia and bind to microtubules (MT), suggesting that

48 disorganized microtubules that fail to enter filopodia and co-align with actin filaments.

49 by siRNA has two major effects: decrease in filopodia and compromised cell-cell adhesion in cells mi

50 The basal filopodia and FN pillars are also necessary for proper s

51 hibition led to a reduction in the number of filopodia and growth cone F-actin content on laminin and

52 eta reduces dynamic movements of growth cone filopodia and impairs presynaptic differentiation.

53 e lower in dendritic spines than in immature filopodia and increased upon expression of a nonadhesive

54 Fascin appears to promote formation of filopodia and invasive activities of PDAC cells.

55 , Enabled, and Capping protein in regulating filopodia and lamellipodia dynamics in Drosophila melano

56 llular matrix-dependent effect, coupled with filopodia and lamellipodia formation and an enrichment o

57 heir amoeboid morphology, the development of filopodia and lamellipodia, and phagocytosis of WNV-infe

58 ith the morphology of their tips, which lack filopodia and lamellipodia.

59 le for glycolytic ATP synthesis localizes in filopodia and lammelipodia, where ATP is rapidly consume

60 sma membrane, FMNL3 enriches particularly in filopodia and membrane ruffles and at nascent cell-cell

61 ubiquitous feature of cell morphology, e.g., filopodia and microvilli, serving a huge variety of func

62 in was induced at contacts between dendritic filopodia and N-cadherin-coated beads or micropatterns.

63 ndicate that NCAM2 promotes the formation of filopodia and neurite branching by inducing Ca(2+) influ

64 f signaling in extreme geometries, including filopodia and primary cilia.

65 F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating ce

66 y, irradiated podocytes demonstrated loss of filopodia and remodeling of cortical actin.

67 3 substrates, TAO kinases regulate dendritic filopodia and spine development, similar to MST3.

68 ling might regulate development of dendritic filopodia and spine synapses is unknown.

69 nstrate that Htt co-localizes with BAIAP2 in filopodia and that mutant HTT interferes with filopodial

70 al FERM domain regulating both the number of filopodia and their elongation velocity.

71 Upon reaching endothelial sprout, it causes filopodia and tip cell retraction.

72 cascade enabled TF trafficking from rafts to filopodia and ultimately onto phosphatidylserine-positiv

73 and cells from mice, fascin concentrated in filopodia and was required for their assembly and turnov

74 ; the growth cones enlarge, extend excessive filopodia, and assume random trajectories.

75 ular injury that likely contribute to spine, filopodia, and dendrite pruning, impacting cognition and

76 t to induce F-actin remodeling, formation of filopodia, and directed cell motility.

77 actin-bundling protein fascin, formation of filopodia, and increased cell motility-all mediators of

78 CD73 associated with cell-cell contacts, filopodia, and membrane zippers, indicative of involveme

79 integrin alphaL toward the cell membrane and filopodia, and secretory vesicles containing the HSP90al

80 he peripheral and central domains, including filopodia, and that SynaptopHluorin signals occur as spo

81 ics of the mitochondria, plasma membrane and filopodia, and the 2D and 3D dynamics of the endoplasmic

82 CP has been postulated to be absent from filopodia, and thus its role in filopodial activity has

83 Commissural filopodia appear on the floor plate to interact with the

84 Dendritic filopodia are actin-filled dynamic subcellular structure

85 Dendritic filopodia are actin-rich structures that are thought to

86 also contribute to filopodial assembly, and filopodia are disproportionately associated with focal a

87 Filopodia are dynamic actin-based structures that play r

88 Invadopodia and filopodia are dynamic, actin-based protrusions contribut

89 Filopodia are dynamic, actin-rich structures that transi

90 Filopodia are essential during dynamic cellular processe

91 Filopodia are exploratory finger-like projections compos

92 Filopodia are finger-like protrusions from the plasma me

93 Filopodia are long plasma membrane extensions involved i

94 sin; MF) in the initiation and elongation of filopodia are not well defined and may reflect conserved

95 Filopodia are thin, fingerlike structures that contain b

96 Filopodia are ubiquitous fingerlike protrusions, spawned

97 called convergent elongation, proposes that filopodia arise from Arp2/3 complex-nucleated dendritic

98 ve observed that human neutrophils use large filopodia as cellular tentacles to sense local environme

99 ngle vesicles to the cell body by surfing on filopodia as well as filopodia grabbing and pulling moti

100 plays in cell motility by implicating CP in filopodia as well as in lamellipodia, both of which are

101 isodic Ca(2+) signals present in both motile filopodia as well as in later-stabilized synaptic bouton

102 le for fascin that operates independently of filopodia assembly to promote efficient cell migration a

103 In neuroblastoma cells, Dyn1 localizes to filopodia, associated tip complexes, and the leading edg

104 and quantify spatiotemporal localization of filopodia-associated proteins during the filopodial exte

105 and separable from a role for fascin within filopodia at the cell periphery.

106 II in actomyosin fibers and the formation of filopodia at the interface of ephrinB1 and EphB2 cells,

107 such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell

108 sequential extension-retraction of platelet filopodia attached to fibrin fibers.

109 AMPs keep their filopodia-based association with muscles throughout thei

110 We suggest that a myosin II-driven, filopodia-based probing mechanism ahead of the leading e

111 either sex to inform modeling of sparse and filopodia-bearing mossy fibers, finding that these circu

112 complex inhibits the formation of dendritic filopodia but that later during development, the Arp2/3

113 ted tubular cell protrusions, reminiscent of filopodia, but extending more than 30 microm into the ex

114 laboratories has shown that IRSp53 generates filopodia by coupling membrane protrusion with actin dyn

115 ular space is by traveling along specialized filopodia called cytonemes.

116 In particular, en passant bouton and filopodia connections with CA3 interneurons peak when ad

117 Filopodia contain cross-linked actin filaments, attached

118 Neuronal growth cone filopodia contain guidance receptors and contribute to a

119 lls, we show that peripheral F-actin bundles/filopodia containing fascin-1 serve as templates for for

120 mice exhibit a 50% decrease in endothelial filopodia, demonstrating that Myo10 is required to form

121 , we found that MST3 is necessary for proper filopodia, dendritic spine, and excitatory synapse devel

122 m by which the guidance cue netrin increases filopodia density is unknown.

123 a molecular heterogeneity whereby different filopodia display markedly different responses to change

124 directed single cell migration, we show that filopodia distribution and their dynamics are dictated b

125 ent, we examined the behavior of growth cone filopodia during the exploration of both correct and off

126 We developed Filopodyan (filopodia dynamics analysis) in Fiji and R to measure fl

127 ediated depletion of Actalpha reduces axonal filopodia dynamics and disturbs collateral branch format

128 ly, our results demonstrate that MYO3A slows filopodia dynamics and enhances filopodia lifetime in CO

129 w, actin within filopodia is responsible for filopodia dynamics by conducting simultaneous force spec

130 Using DiI-SiR, we visualized filopodia dynamics in HeLa cells over 25 min at 0.5 s te

131 tomyosin-driven mechanism controls dendritic filopodia dynamics.

132 vidual regulatory and structural proteins as filopodia elongate and subsequently retract.

133 myosin X then initiates the second cycle of filopodia elongation to produce the long filopodia.

134 d to be capable of rescuing the formation of filopodia, establishing the minimal elements necessary f

135 a model we show that >90% of the growth cone filopodia exhibit fast, stochastic dynamics that persist

136 nterdigitated membrane surfaces, with T cell filopodia extending toward virions sequestered deep insi

137 ch is known about the molecules that control filopodia extension and subsequent maturation into funct

138 During filopodia extension, we found the translocation of Arp2/

139 howed delayed vascular outgrowth and reduced filopodia extension, which are both VEGF-A-dependent pro

140 sin X (encoded by Myo10)-induced multi-cycle filopodia extension.

141 Furthermore, cells used filopodia extensions to probe substrate rigidity at a di

142 Macrophage filopodia, finger-like membrane protrusions, were first

143 Surprisingly, filopodia form and elongate toward sources of Slit, a re

144 is necessary for CDC42 activation to signal filopodia formation and depends on the activation of RHO

145 t the CT region contributes to DAAM-mediated filopodia formation and dynamics in primary neurons.

146 on underlies diverse molecular mechanisms of filopodia formation and extension.

147 is associated with the mechanism underlying filopodia formation and extension.

148 e engineered MYO6+ offers insights into both filopodia formation and MYO6 motor function at endosomes

149 of ATP drives EC rearrangement by promoting filopodia formation and reducing intercellular adhesion.

150 time-lapse imaging to reveal a high level of filopodia formation and retraction on the distal dendrit

151 carcinoma in situ, we reveal a link between filopodia formation at the cell-matrix interface, in col

152 interaction-defective mutant of Pak2 rescued filopodia formation but led to abnormal F-actin bundles.

153 vated kinase (PAK)-mediated lamellipodia and filopodia formation following bradykinin or PDGF stimula

154 fy the recruitment of ENA and VASP preceding filopodia formation in neuronal growth cones, and uncove

155 uired for embryonic axonal morphogenesis and filopodia formation in the growth cone.

156 Defective filopodia formation is linked to pathologies such as can

157 he extracellular Mg(2+) concentration led to filopodia formation on the dendrites of mature adult-bor

158 c42, which modulate actin polymerization and filopodia formation via the Arp2/3 complex and Diaphanou

159 ficantly delayed angiogenesis, tip cell, and filopodia formation, a phenomenon associated with activa

160 e involved in processes such as endocytosis, filopodia formation, and cell migration.

161 ition reverses the elevated F-actin content, filopodia formation, and matrix degradation induced by P

162 TRXR and inflammasome activity promoted filopodia formation, cellular release of reduced TRX, an

163 raction with beta-Pix are essential for HSPC filopodia formation, cytoskeletal integrity, and homing

164 By specifically interfering with filopodia formation, we demonstrate for the first time t

165 PA gel required Cdc42- and formin-dependent filopodia formation, whereas adhesion to a 60-kPa Ecad-F

166 g and sprouting, as well as markedly reduced filopodia formation.

167 s reducing actin polymerization required for filopodia formation.

168 vity and cofilin phosphorylation, decreasing filopodia formation.

169 t, concurrent with a decrease in the rate of filopodia formation.

170 itate the recruitment of Fascin and initiate filopodia formation.

171 icantly attenuated the myosin-X-induced long filopodia formation.

172 lateral movement, and subsequently initiated filopodia formation.

173 up formation, but displayed markedly reduced filopodia formation.

174 diated by GIPC and PI(4,5)P2 are crucial for filopodia formation.

175 Filopodia formation/retraction on the dendrites of adult

176 ich the principal cells' activity influences filopodia formation/retraction on the dendrites of inter

177 Whether filopodia formed by different molecular mechanisms equal

178 cues in the microenvironment, implying that filopodia foster local signal transduction, yet their sm

179 the formation and elongation of actin-based filopodia from mouse dorsal root ganglion growth cones.

180 l glia in the developing brain extend motile filopodia from their primary stalk.

181 rin-beta then became localized at the tip of filopodia, from where myosin X initiated the second exte

182 te wide biological significance, delineating filopodia function in complex systems remains challengin

183 Moreover, in the absence of Ena/VASP, filopodia generated by mDia2 did not support initiation

184 cell body by surfing on filopodia as well as filopodia grabbing and pulling motions to reach endocyti

185 ding fixation, (ii) capturing at the tip and filopodia-guided actin anterograde flow with phagocytic

186 Filopodia have important sensory and mechanical roles in

187 d-end actin polymerization and can stimulate filopodia in cultured cells.

188 ity, we collected and analyzed image data on filopodia in cultured rat hippocampal neurons.

189 as Cdc42 is required for the predominance of filopodia in early neurulation.

190 e is widely applicable, capable of detecting filopodia in four different cell types in vitro and in v

191 TPIP1 regulates the balance of podosomes and filopodia in macrophages.

192 olecular motor that localizes to the tips of filopodia in mammalian cells.

193 The formation of filopodia in Metazoa and Amoebozoa requires the activity

194 porter (DAT) has been shown to accumulate in filopodia in neurons and non-neuronal cells.

195 led multiple mechanisms involving macrophage filopodia in particle capture and engulfment.

196 We examined embryonic growth cone filopodia in vivo to directly observe their exploration

197 opy, we observe that tumor spheroids display filopodia in vivo, supporting a potential role for these

198 Myo10 is required to form normal numbers of filopodia in vivo.

199 y, electron microscopy experiments on mature filopodia indeed frequently reveal actin bundles that ar

200 To explore the role of filopodia-inducing Cdc42, we generated myeloid-restricte

201 Here, we demonstrate a role for the filopodia-inducing motor protein Myosin-X (Myo10) in mut

202 Filopodia initiated by either Ena/VASP or mDia2 containe

203 anges in ApoER2 abundance modulate dendritic filopodia initiation and synapse maturation.

204 Here, we find that filopodia initiation is suppressed by recruitment of Arh

205 The dynamics of filopodia interacting with the surrounding extracellular

206 low underlies the stabilization of dendritic filopodia into mature spines, a mechanism that may have

207 Fascin promoted intercalation of filopodia into mesothelial cell layers and cell invasion

208 nt-based structures, including lamellipodia, filopodia, invadopodia, and membrane blebs, as well as o

209 The exploratory motion of the filopodia is crucial for synaptogenesis, but the underly

210 sexes revealed that the formation of ectopic filopodia is increased in Sema2a heterozygotes.

211 investigated whether, and how, actin within filopodia is responsible for filopodia dynamics by condu

212 the dimensions of actin-rich processes like filopodia, lamellipodia, microvilli, and stereocilia req

213 filaments, comprising the structural core of filopodia, largely determine their instantaneous lengths

214 MYO3A slows filopodia dynamics and enhances filopodia lifetime in COS7 cells.

215 M10(Full)LZ localizes at the tip of filopodia like myosin-X full-length (M10(Full)).

216 zation and increased proportions of immature filopodia-like dendritic protrusions at the expense of t

217 ng, and redistribution of Ena to the tips of filopodia-like extensions.

218 led filaments, facilitating the formation of filopodia-like F-actin networks without tapered barbed e

219 , fibroblast cells adopt a leading edge with filopodia-like protrusions (FLPs) and maintain an abilit

220 a freely available ImageJ plugin, to detect filopodia-like protrusions in both fixed- and live-cell

221 Taken together, our data suggest a role of filopodia-like protrusions in mediating signaling events

222 These filopodia-like protrusions span the subectodermal space

223 , and shape-changing vesicles with streaming filopodia-like protrusions.

224 EGFP macrophages, we show that filopodia, or filopodia-like structures, support pathogen clearance by

225 besides lamellipodia, including lamella and filopodia, may have unappreciated roles in cell migratio

226 nd that plasma-membrane protrusions, such as filopodia, may serve as specialized metastatic engines.

227 pillars are specifically established through filopodia-mediated and pulsating force-related mechanism

228 Impeding filopodia-mediated nucleation and micron-scale assembly

229 Adhesions were formed by filopodia-mediated nucleation and micron-scale assembly

230 scopy is capable of imaging morphogenesis of filopodia, membrane ruffles, pit formation, and endocyto

231 le promoting Rac activation and formation of filopodia (microspikes).

232 e different actin regulatory proteins affect filopodia morphology and dynamics independently of one a

233 Marginally located nucleocapsids entered filopodia, moved inside, and budded from the tip of thes

234 ctivity (e.g., endothelial tip cell density, filopodia number) can be obtained.

235 (2) neurite complexity, and (3) growth cone filopodia number, in accordance with CD2AP expression le

236 FMNL3-containing filopodia occur both at the cell-substratum interface an

237 Pleomorphic spines and nonsynaptic filopodia occur in the MePD.

238 s substrates, cortactin, in lamellipodia and filopodia of Aplysia growth cones.

239 by optical tweezers to the ends of adherent filopodia of J774 macrophages, are transported discontin

240 actin filaments and dynamic microtubules in filopodia of pseudopods of invading cells under a chemot

241 Ena cooperate to extend and maintain robust filopodia of uniform thickness with aligned barbed ends

242 as sufficient to induce the formation of new filopodia on the distal dendrites of immature adult-born

243 copy, we show that Dyn1 and Mena localize to filopodia only during initiation and assembly.

244 ng in radial glia in vivo to eliminate glial filopodia or impair glial motility profoundly impacted s

245 Cdc42 is not critical for filopodia or phagocytic cup formation, but plays a key r

246 stablishes a role for specialized signalling filopodia, or cytonemes, in morphogen dispersion and sig

247 ident Lifeact-EGFP macrophages, we show that filopodia, or filopodia-like structures, support pathoge

248 ational model integrating four modules of 1) filopodia penetration dynamics; 2) intracellular mechani

249 Filopodia perform cellular functions such as environment

250 or to lumen formation, whereas proximal, tip filopodia persist in the absence of DOCK4.

251 rocess in which protrusion and retraction of filopodia play a key role.

252 ometric imaging reveals that F-actin bundles/filopodia play both structural and signaling roles, as t

253 ay facilitate actin filament convergence for filopodia production.

254 ed by lack of compatible methods to quantify filopodia properties.

255 Alongside the FN pillars, long filopodia protrude from the basal surfaces of somite epi

256 Filopodia protrude from the leading edge of cells and pl

257 n as well as detailed interactive editing of filopodia reconstructions through an intuitive graphical

258 In contrast, a subset of filopodia remains after Arp2/3 complex inhibition in adh

259 We demonstrate that filopodia repeatedly contact off-target muscles over sev

260 Motoneuron filopodia repeatedly contacted off-target muscle fibers

261 le cells from neuromorpho.org with spine and filopodia segments stochastically distributed along the

262 ity induces directional growth of spine head filopodia (SHF) followed by spine relocation.

263 Predicted average filopodia speed and that of the cell membrane advance ag

264 se and, consequently, promotes the dendritic filopodia-spines (F-S) transition.

265 soforms, Actalpha, Actbeta, and Actgamma, to filopodia stability and dynamics during this process is

266 This filopodia stochastic model is integrated into migratory

267 en actin filament bundles and dynamic MTs in filopodia, suggesting that tau links these two cytoskele

268 re distributed along the length of astrocyte filopodia, suggesting that virus transfer to the astrocy

269 Filopodia supported the phagocytic uptake of bacterial (

270 In addition, filopodia supported the uptake of zymosan (Saccharomyces

271 We found that nonmigratory AS cells produce filopodia that are morphologically and dynamically disti

272 dorsal-ventral sarcomere region and develops filopodia that elongates anteriorly towards the spicule

273 Migrating cells typically form filopodia that extend from the cell surface, but the pre

274 le at their leading edge, as well as dynamic filopodia that finally knit the wound edges together.

275 tered localization of the remaining Ezrin to filopodia that form during activation.

276 n with the emergence of actin-rich dendritic filopodia that initiate contact with presynaptic axonal

277 We report that emerging AMPs send out thin filopodia that make contact with neighboring muscles.

278 ation in ECM to predict dynamic behaviors of filopodia that penetrate into a 3D ECM fiber network.

279 ther facilitate FGF22 targeting to dendritic filopodia that receive presynaptic stimulation.

280 icit local Ca(2+) signals within growth cone filopodia that regulate axon guidance through activation

281 essential role for cytonemes as specialized filopodia that transport signaling proteins between sign

282 show Kirrel3 is required for formation of MF filopodia; the structures that give rise to DG-GABA syna

283 N pillar formation is dependent on the basal filopodia through these molecules.

284 Then myosin X moves to the filopodia tip and attracts integrin-beta and Arp2/3 for

285 s (30-34) in the MYO3A tail does not prevent filopodia tip localization but abolishes the ability to

286 M9, an E3 ubiquitin ligase that localizes to filopodia tips and binds the netrin receptor DCC, intera

287 ll shape, including protrusions ranging from filopodia to lamellipodia.

288 lex drives the morphological maturation from filopodia to typical spine morphology.

289 rtebrate central nervous system, exploratory filopodia transiently form on dendritic branches to samp

290 rget molecule abundance, it is revealed that filopodia typically harbor higher densities of 3' phosph

291 We devised a scheme to estimate the radii of filopodia using either the membrane marker or volume mar

292 oskeleton protrusions-i.e., lamellipodia and filopodia-were reduced after treatment.

293 s, actin filaments redistribute to extending filopodia where they exhibit increased dynamics.

294 bound R60A mutant predominantly localized in filopodia, whereas free R60A molecules were evenly distr

295 n cytoskeletal protrusion structures are the filopodia, which act like cell sensory organs to communi

296 must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound

297 lls can interact with their surroundings via filopodia, which are membrane protrusions that extend be

298 minus (GIPC) accumulate at the tips of these filopodia, while APPL1 endosomes accumulate at the base.

299 e myosin X initiated the second extension of filopodia with a change in extension direction, thus pro

300 Ena/VASP and mDia2 support the formation of filopodia with significantly distinct properties and tha