ライフサイエンスコーパス: fimbrial

1 sequence of the agg5DCBA gene cluster shared fimbrial accessory genes (usher, chaperone, and minor pi

2 luated the function of Mfa5, one of the Mfa1 fimbrial accessory proteins.

3 nic Escherichia coli expressing the K88 (F4) fimbrial adhesin (K88 ETEC) is a significant source of m

4 ia the colonization factor antigen I (CFA/I) fimbrial adhesin CfaE.

5 ructure was resolved based on fimH sequence (fimbrial adhesin gene: H subclone assignments), multiloc

6 lele III (which encodes variant III of the P-fimbrial adhesin molecule PapG).

7 fumC) but similar to that seen with another fimbrial adhesin of E. coli, papG-II, also implicated in

8 FimH, the mannose-specific, type 1 fimbrial adhesin of Escherichia coli, acquires amino aci

9 Here, by using a common mannose-specific fimbrial adhesin of Escherichia coli, FimH, we demonstra

10 The std operon encodes a fimbrial adhesin of Salmonella enterica serotype Typhimu

11 t not primary) structure to FimH, the type 1 fimbrial adhesin of uropathogenic E. coli, which shows s

12 it belongs) to genes that encode a putative fimbrial adhesin required for biofilm formation.

13 The non-fimbrial adhesin ShdA is a fibronectin binding protein r

14 niformly contained allele 41 of fimH (type 1 fimbrial adhesin) and a narrow range of alleles of gyrA

15 brial structural subunit), papG allele II (P fimbrial adhesin), iha (putative adhesin siderophore), a

16 host-associated nsSNPs for FimH, the type 1 fimbrial adhesin, highlights the role of key allelic res

17 fimH, which encodes mannose-specific type 1 fimbrial adhesin, resulting in functionally distinct cop

18 date structure and function of this class of fimbrial adhesins and assess the feasibility of an adhes

19 K88 fimbrial adhesins are filamentous surface appendages who

20 A range of fimbrial adhesins in ETEC strains determines host and ti

21 have centered on a subset of plasmid-encoded fimbrial adhesins known as colonization factors and heat

22 Fimbrial adhesins mediate the attachment of pathogenic E

23 of urinary tract infections (UTI), utilizes fimbrial adhesins to colonize the uroepithelium.

24 icularly rich in genes that encode potential fimbrial adhesins, autotransporters, iron-sequestration

25 acterization of the fimH genes, encoding the fimbrial adhesins, indicated two allelic variants.

26 decreased stress and elevated expression of fimbrial adhesins.

27 tively encode P and type 1 receptor-specific fimbrial adhesins.

28 rsubunit interface and significantly reduced fimbrial adhesion in this assay.

29 Thus, inhibiting P-fimbrial adhesion may reduce the incidence of UPEC-media

30 The Escherichia coli fimbrial adhesive protein, FimH, mediates shear-dependen

31 o monomannose-coated surfaces via the type 1 fimbrial adhesive subunit, FimH.

32 r anaerobic conditions, the transcription of fimbrial and EPS enzymes was elevated in both Rv and Sv

33 Here we compare the DNA methylome of the fimbrial and proximal ends of the fallopian tube in BRCA

34 nt differences in methylation levels between fimbrial and proximal fallopian tube segments are threef

35 actic but showed upregulation of a number of fimbrial and Salmonella pathogenicity island 3 (SPI-3) a

36 STIC was typically fimbrial and unifocal, with variable invasion of the tub

37 sly unidentified transcriptional regulators, fimbrial, and sugar phosphotransferase homologues, as we

38 , we show using force spectroscopy that anti-fimbrial antibodies diminish fimbrial elasticity by inhi

39 ly, passive oral administration of ETEC anti-fimbrial antibodies prevent ETEC diarrhea.

40 The interaction of the minor fimbrial antigen (Mfa) with streptococcal antigen I/II (

41 he streptococcal antigen I/II with the minor fimbrial antigen (Mfa1) of P. gingivalis.

42 FimA was the only fimbrial antigen expressed by S. Typhimurium after stati

43 us gordonii through interaction of the minor fimbrial antigen Mfa1 with a specific region of the stre

44 ion of C3H/HeJ mice with Escherichia coli Dr fimbrial antigen reduced mortality associated with an ex

45 nditions drastically alter the repertoire of fimbrial antigens expressed in S. Typhimurium.

46 To study the expression of fimbrial antigens in S.

47 that the expression of serotype Typhimurium fimbrial antigens is induced during the infection of mic

48 Among the seven most prevalent fimbrial antigens of human ETEC, CS4 is the last to be c

49 e deletion mutants for gating, expression of fimbrial antigens was measured by flow cytometry in cult

50 mals seroconverted to only a subset of these fimbrial antigens.

51 ions with regard to expression of long polar fimbrial antigens.

52 The fimbrial appendages are found on the surface of many ent

53 hat the tip fimbrillin, FimQ, is involved in fimbrial assembly and interaction with PRPs.

54 colonization factors of the chaperone/usher fimbrial assembly class; however, the binding specificit

55 One way of controlling fimbrial assembly in these bacteria is at the genetic le

56 tigation that are necessary to elucidate the fimbrial assembly pathways in Actinomyces and their func

57 The transcription of tadV (fimbrial assembly), pgaC (extracellular polysaccharide s

58 nd gap3 mutants in terms of Fap1 biogenesis, fimbrial assembly, and bacterial adhesion, suggesting th

59 h these isopeptide bonds are dispensable for fimbrial assembly, cell-cell interactions and biofilm fo

60 ed serine-rich glycoprotein, is required for fimbrial assembly.

61 fimbriae, it also plays an important role in fimbrial assembly.

62 s between the structure of FaeG proteins and fimbrial binding function, and perhaps virulence in dise

63 Such variant specificity in fimbrial binding is believed to be controlled by the maj

64 mbly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads.

65 es of the FasG adhesin are important in 987P fimbrial binding to sulfatide and glycoprotein receptors

66 fimbria-associated adhesin, is required for fimbrial biogenesis and biofilm formation in Streptococc

67 a serine-rich glycoprotein, is essential for fimbrial biogenesis and biofilm formation of Streptococc

68 gene clusters encode similar components for fimbrial biogenesis but different types of regulators fo

69 es the SPI-1/SPI-2 virulence, flagellar, and fimbrial biogenesis pathways.

70 lved in iron acquisition, antibiotic efflux, fimbrial biogenesis, and pyocin synthesis.

71 dinate regulation of genes involved in F1845 fimbrial biogenesis.

72 ed to as an usher because of its function in fimbrial biogenesis.

73 ardless of whether the strain carried intact fimbrial biosynthesis genes.

74 n carcinomas', which frequently develop from fimbrial cells but not from the proximal portion of the

75 being developed, many of which target common fimbrial colonization factors as the major constituent,

76 of ETEC strains, their serotypes, and their fimbrial components have been well studied.

77 to investigate the relative contributions of fimbrial deformation and bond transitions to the rolling

78 he low-affinity bond number due to increased fimbrial deformation with shear.

79 espondingly, FimA protein production and the fimbrial display on the mutant were significantly reduce

80 Thus far, no fimbrial domain(s) that facilitates cell invasion has be

81 scopy that anti-fimbrial antibodies diminish fimbrial elasticity by inhibiting their natural capacity

82 alent antibody binding is required to reduce fimbrial elasticity.

83 possible mechanism is implantation of normal fimbrial epithelium on the denuded ovarian surface at th

84 ion of activation-induced deaminase in their fimbrial epithelium.

85 ht into the complex interplay between type 1 fimbrial expression and flagellum-mediated motility.

86 ppeared that the relationship between type 1 fimbrial expression and motility is unidirectional, wher

87 RosE is a novel negative regulator of Std fimbrial expression as indicated by its repression of a

88 L-6 production and suggesting that the CFA/I fimbrial expression by Salmonella may protect against a

89 in expression (P < 0.0001), a loss of type 1 fimbrial expression did not result in increased motility

90 Type 1 fimbrial expression in these mutants was unaltered, and

91 vaT and MvaU on pyocyanin synthesis and CupA fimbrial expression in these mutants were also analyzed.

92 reased cell density itself, this increase in fimbrial expression is due to an enrichment of fimbriate

93 ese data suggest that a tight control of Std fimbrial expression mediated by RosE is required during

94 CFT073 by examining the phenotypic effect of fimbrial expression on motility and the effect that indu

95 The effect of the mrkJ mutation on type 3 fimbrial expression was shown to be at the level of mrkA

96 the relationship between motility and type 1 fimbrial expression was tested for UPEC strain CFT073 by

97 on of motility caused by constitutive type 1 fimbrial expression, it was concluded that the synthesis

98 ect that induction of motility has on type 1 fimbrial expression.

99 cyclic-di-GMP (c-di-GMP) and regulate type 3 fimbrial expression.

100 heterologous colonization factors while anti-fimbrial Fab fractions showed HAI activity limited to co

101 Fimbrial filaments assembled by distinct chaperone pathw

102 uced into Escherichia coli and expression of fimbrial filaments composed of PefA confirmed by flow cy

103 in expression of StdA and its assembly into fimbrial filaments on the cell surface.

104 n bearing a phenotypic variant of the type 1 fimbrial FimH adhesin characteristic of those prevalent

105 diate mannose-sensitive adhesion, the type 1 fimbrial FimH adhesins of Salmonella Typhimurium and Esc

106 determine which components are required for fimbrial formation in S. Typhimurium, mutations in fimA,

107 a molecular connection between flagella and fimbrial formation in serovar Typhimurium, indicating th

108 Both fimbrial forms are composed of 5 proteins, but there is

109 surface, suggesting that dispersin's role in fimbrial function is to overcome electrostatic attractio

110 tinal antibodies in mechanical disruption of fimbrial function may provide insights relevant to ETEC

111 Immediately adjacent to the type 3 fimbrial gene cluster is a gene, mrkJ, that is related t

112 this fimbrial usher gene is part of a novel fimbrial gene cluster, aufABCDEFG.

113 ase-variable (ON/OFF) expression of the cupA fimbrial gene cluster.

114 eruginosa adhesins encoded by three distinct fimbrial gene clusters (cupA, cupB and cupC).

115 In this study we characterized two fimbrial gene clusters present in the genome of Actinomy

116 chanisms that have been described to control fimbrial gene expression and uses specific examples to d

117 regulatory link between flagellar and type 1 fimbrial gene expression dynamics, where we found that t

118 In addition to EA, choline activated fimbrial gene expression in EHEC.

119 n mRNA coordinates a hierarchical control of fimbrial gene expression in S. typhimurium.

120 A hierarchical control of fimbrial gene expression limits laboratory grown culture

121 ing the EHEC secreted effectors and putative fimbrial gene expression showed a variable expression pr

122 flagellar regulator, FliZ, represses type 1 fimbrial gene expression through the posttranscriptional

123 marcescens biofilm formation by influencing fimbrial gene expression.

124 enced EHEC adherence to epithelial cells and fimbrial gene expression.

125 also components of cell membranes, activated fimbrial gene expression.

126 level by positively or negatively regulating fimbrial gene expression.

127 , srlE, garP, fes, and cirA), along with the fimbrial gene papB.

128 PapX, a P fimbrial gene product of UPEC strain CFT073, is a functi

129 genome contains 13 operons with homology to fimbrial gene sequences.

130 an opposite to those seen with nonstructural fimbrial genes (fimC and fimI) and housekeeping loci (ad

131 or the phase-variable expression of the cupA fimbrial genes and suggest that CgrC exerts its regulato

132 Our results showed that the use of these fimbrial genes as markers, in combination with the diffe

133 Our findings suggest that fimbrial genes important for biofilm formation can be ex

134 ossibility that phase-variable expression of fimbrial genes important for biofilm formation may occur

135 Deletion of these fimbrial genes increased motility.

136 Transcription of type 1 fimbrial genes is controlled by a promoter that resides

137 tion of a phase variation module to regulate fimbrial genes is proposed to be beneficial for the adap

138 Mutations in oxyR and type I fimbrial genes resulted in severe defects in fimbria-ass

139 Mutations in type 1 fimbrial genes resulted in severely attenuated colonizat

140 re the phase-variable expression of the cupA fimbrial genes to cells of a cgrC mutant strain.

141 The fimbrial genes ygiL, yadN, yfcV, and c2395 were signific

142 Surprisingly, other fimbrial genes, such as pap and foc/sfa, and genes invol

143 sion dynamics of flagellar, SPI1, and type 1 fimbrial genes.

144 xpression of the flagellar, SPI1, and type 1 fimbrial genes.

145 y the SPI1 genes, and ending with the type 1 fimbrial genes.

146 or the phase-variable expression of the cupA fimbrial genes.

147 the sequence of PsaA, the subunit of the Psa fimbrial homopolymer, identified residues that abolish g

148 Therefore, we examined the ability of P fimbrial isogenic mutants, constructed in a type 1 fimbr

149 mannose-containing oligosaccharides with the fimbrial lectin of E. coli to design novel biosensors.

150 t time the transcription of several putative fimbrial loci in EHEC.

151 ts in IJ colonization revealed that a single fimbrial locus, named mad for maternal adhesion defectiv

152 The transcription of fimbrial, LPS, and EPS genes in the Rv was increased app

153 surface protein-encoding loci, including the fimbrial major subunit gene, fim2.

154 Importantly, the expression of the non-fimbrial matrix materials was affected by all mutations,

155 regulated the surface exposure of the minor fimbrial (Mfa) protein subunit of P. gingivalis, which s

156 ders were inoculated with the wild type or a fimbrial mutant and in coinfection studies in which the

157 ulticopy oxyR plasmid was absent in a type I fimbrial mutant background.

158 Complementation of each fimbrial mutant restored wild-type levels of motility, b

159 Fimbrial mutants showed the greatest reduction in bindin

160 To test this hypothesis, the virulence of fimbrial mutants was assessed in independent challenges

161 infection studies in which the wild type and fimbrial mutants were inoculated together to assess the

162 al isogenic mutants, constructed in a type 1 fimbrial-negative background, to compete in the murine u

163 nesis was performed on a phase-locked type 1 fimbrial ON variant of strain CFT073 (CFT073 fim L-ON),

164 TcfB is the major structural subunit of a fimbrial operon found in serovar Typhi with no homolog i

165 R indicated an absence of transcripts from a fimbrial operon in an oxyR mutant that were present in t

166 otility, is encoded at the 3' end of the mrp fimbrial operon in P. mirabilis.

167 The lpf fimbrial operon oscillates between phase ON and phase OF

168 ween host range and the presence of a single fimbrial operon seems at present unlikely.

169 ve autotransporters, an extension of the atf fimbrial operon to six genes, including an mrpJ homolog,

170 t induced expression of the putative stdABCD fimbrial operon were identified from a random bank of tr

171 lmonella Pathogenicity Island 1 (SPI-1), ste fimbrial operon, and Clustered, Regularly Interspaced, S

172 nts, each carrying a deletion of a different fimbrial operon, for their enteropathogenicity using the

173 regulator MrpJ, which is encoded by the mrp fimbrial operon, has been shown to repress both swimming

174 lic AMP (cAMP)-dependent activation of the P fimbrial operon.

175 Mutations were found in: (i) the type 1 fimbrial operon; (ii) genes involved in the biosyn-thesi

176 al genes within SPI6, all of SPI9, and three fimbrial operons (fim, bcf, and stb) were conserved with

177 Collectively, these data show that six fimbrial operons (lpf, bcf, stb, stc, std, and sth) cont

178 collection B showed that all eight putative fimbrial operons of serotype Typhi were also present in

179 The effect of deletion of Ygi and Yad fimbrial operons on growth, motility, biofilm formation,

180 Typhimurium) genome contains 13 putative fimbrial operons termed agf (csg), fim, pef, lpf, bcf, s

181 nome contains a large repertoire of putative fimbrial operons that remain poorly characterized becaus

182 onephritis isolate E. coli CFT073 carries 12 fimbrial operons, 5 of which have never been studied.

183 Here we characterized the role of 10 fimbrial operons, agf, fim, lpf, pef, bcf, stb, stc, std

184 s with deletions of one or both of the two P fimbrial operons, hlyA, fliC, ibpB, c0468, locus c3566 t

185 two previously uncharacterized EA-regulated fimbrial operons, loc10 and loc11.

186 Interestingly, only one of the two P fimbrial operons, pap_2, present in the genome of E. col

187 eight new putative chaperone-usher-dependent fimbrial operons, which were termed bcf, sta, stb, ste,

188 ncodes several non-LEE-encoded effectors and fimbrial operons.

189 ues of mrpJ, 10 of which are associated with fimbrial operons.

190 stically to limit coexpression of homologous fimbrial operons.

191 n of the genes in characterized and putative fimbrial operons.

192 t contained a unique combination of putative fimbrial operons.

193 d January 2007, all (100%) originated in the fimbrial or ampullary region of the tube; six had an ear

194 ETEC organisms express fimbrial (or fibrillar) colonization factor antigens tha

195 tions is a result of both selection (of MR/P fimbrial phase variants) and signaling (via modulation o

196 The inversion determines CS18 fimbrial phase variation.

197 Type 1 fimbrial phase-locked mutants of E. coli strain CFT073,

198 Type 1 fimbrial phase-locked mutants of uropathogenic Escherich

199 sing an intact mrk gene cluster restored the fimbrial phenotype and the rapid ability to form biofilm

200 on an OxyR-deficient strain and an enhanced fimbrial phenotype in strains bearing oxyR on a multicop

201 Fimbrial polymerization requires the fimbria-specific so

202 ndicating that these genes are necessary for fimbrial production in S. Typhimurium.

203 sly unknown role for cyclic-di-GMP in type 3 fimbrial production.

204 exhibiting a four- to eightfold increase in fimbrial production.

205 xist that generally contain common toxin and fimbrial profiles, with many of the isolates belonging t

206 ed mice showed that vaccination with the Psa fimbrial protein together with an adjuvant afforded inco

207 its lipopolysaccharide (LPS), and its major fimbrial protein, fimbrillin (FimA).

208 its lipopolysaccharides (LPS), and its major fimbrial protein, fimbrillin (FimA).

209 n (PT), filamentous hemagglutinin (FHA), and fimbrial proteins (FIM) were measured by validated IgG-s

210 HfaA is similar to fimbrial proteins and assembles into a high molecular we

211 wn P. gingivalis virulence factors including fimbrial proteins and gingipains.

212 S. Typhimurium type 1 fimbrial proteins are encoded by the fim gene cluster (f

213 Evidence for in vitro expression of fimbrial proteins encoded by these operons is currently

214 MUB domains showed structural similarity to fimbrial proteins from Gram-positive pathogens, the part

215 stigated the role of 11 serotype Typhimurium fimbrial proteins, including FimA, AgfA (CsgA), BcfA, St

216 d includes iron-uptake systems, adhesins and fimbrial proteins.

217 In the mouse bladder, the E. coli type 1 fimbrial receptor, uroplakin Ia, was located in lipid ra

218 proline-rich proteins (PRPs), which serve as fimbrial receptors, involves type 1 fimbriae.

219 ere augmented, while secreted IgG anti-CFA/I fimbrial responses remained unaffected compared to those

220 (-/-) mice, mucosal and serum IgA anti-CFA/I fimbrial responses were augmented, while secreted IgG an

221 highlights the importance of the nonadhesive fimbrial rod for adhesive function.

222 und to be dependent on the background of the fimbrial shaft (E. coli versus K. pneumoniae) rather tha

223 the MrkD adhesin that is associated with the fimbrial shaft comprised of the MrkA protein.

224 rtC1, the type 1 fimbria is comprised of the fimbrial shaft FimP and the tip fimbrillin FimQ.

225 FimH subunits, but are also modulated by the fimbrial shaft on which each FimH subunit is presented,

226 ial binding was not a function of the type I fimbrial shaft or the presence of other types of fimbria

227 haft, e.g. K. pneumoniae FimH on the E. coli fimbrial shaft or vice versa, altered the binding specif

228 nd that expression of FimH on a heterologous fimbrial shaft, e.g. K. pneumoniae FimH on the E. coli f

229 rmational constraints imposed on FimH by the fimbrial shaft.

230 The capacity of type 1 fimbrial shafts to modulate the tissue tropism of differ

231 hat included the F16 or F7-2 papA alleles (P fimbrial structural subunit), papG allele II (P fimbrial

232 The fimbrial structural unit, Fap1, is indispensable for fim

233 fimQ-fimP-srtC1-fimR gene cluster encodes a fimbrial structure (designated type 1) that contains a m

234 B-fimA-srtC2 gene cluster encodes a distinct fimbrial structure (designated type 2) composed of a sha

235 These include proteins implicated in fimbrial structure and biogenesis, antimicrobial resista

236 aeruginosa encodes components of a putative fimbrial structure that enable this opportunistic human

237 omponents and assembly factors of a putative fimbrial structure that enable this opportunistic pathog

238 Despite being the most common fimbrial structure, relatively little is known regarding

239 Moreover, putative fimbrial structures were produced by EHEC cells grown wi

240 tion of fimP completely abolished the type 1 fimbrial structures, surface display of monomeric FimQ w

241 ssess two lpf loci encoding highly regulated fimbrial structures.

242 The csa operon encodes a 17-kDa major fimbrial subunit (CsaB), a 40-kDa tip-associated protein

243 t), cytolethal distending toxin (cdt), major fimbrial subunit (flp-1), and serotype-specific O polysa

244 because the genes coding for the only other fimbrial subunit are identical among the three variants.

245 , these studies showed that retention of K99 fimbrial subunit as native fimbriae or with the deletion

246 rmined, providing the first atomic view of a fimbrial subunit assembled by the alternate chaperone pa

247 As placement of the K99 fimbrial subunit became progressively contained within t

248 the enterotoxigenic Escherichia coli (ETEC) fimbrial subunit CfaB (CFA/I structural subunit) alone o

249 oning and orientation of the subjacent major fimbrial subunit CfaB in the native assembly of CFA/I fi

250 Hence, the Mfa1 structural fimbrial subunit does not require accessory proteins for

251 s in hydrophilic domain II of the structural fimbrial subunit DraE and evaluated recombinant mutant D

252 s protective, we questioned whether altering fimbrial subunit expression to resemble conventional Sal

253 lmonella-CFA/I vaccine was modified to limit fimbrial subunit expression to the intracellular compart

254 The Bvg-regulated promoters for the fimbrial subunit genes fim2 and fim3 of Bordetella pertu

255 ure of the promoters of Bordetella pertussis fimbrial subunit genes fim2, fim3 and fimX is the presen

256 ulting in diminished localization of the K99 fimbrial subunit in the outer membrane.

257 When the main fimbrial subunit of Actinomyces naeslundii type I fimbri

258 Fusion proteins between the major fimbrial subunit of thin curled fimbriae (CsgA) and glut

259 train includes three genes, fimQ for a minor fimbrial subunit that appears to be an adhesin, fimP for

260 Thus, this structure depicts a fimbrial subunit that forms a polymeric adhesin with a u

261 nDEFGH, which also reduced the export of the fimbrial subunit to the outer membrane but retained more

262 nGH, which greatly reduced the export of the fimbrial subunit to the outer membrane, showed only part

263 ), the promoter for the virulence gene fim3 (fimbrial subunit), using gel retardation, potassium perm

264 ap1 adhesin has been identified as the major fimbrial subunit, and recent studies suggest that Fap1 i

265 isolates did not possess any known AAF major fimbrial subunit, despite the presence of other AggR-rel

266 ase analysis of the genes encoding the major fimbrial subunits demonstrated that they are present in

267 orphisms and the classification of the major fimbrial subunits into distinct variants.

268 We raised antisera against putative major fimbrial subunits of S.

269 One feature of DraE and other fimbrial subunits that makes them peculiar among Ig-like

270 e SrtC1, which catalyzes covalent linkage of fimbrial subunits.

271 evealed that these genes also altered type 3 fimbrial surface expression in K. pneumoniae.

272 as constructed and shown to be deficient for fimbrial surface expression under aerobic conditions.

273 tion mutants which were deficient for type 3 fimbrial surface production.

274 among stool strains, with orientation of the fimbrial switch being both in the "off" position and in

275 of the fim operon that affected the rate of fimbrial switching from the 'off' to the 'on' phase.

276 ifferences between the S. Typhimurium type 1 fimbrial system and the E. coli type 1 and Pap fimbrial

277 se share distant evolutionary relatedness to fimbrial systems of three other genera.

278 mbrial system and the E. coli type 1 and Pap fimbrial systems.

279 These findings indicate that the dscCfaE fimbrial tip adhesin serves as a protective passive vacc

280 investigate the potential of MrpH, the MR/P fimbrial tip adhesin, as a vaccine, the mature MrpH pept

281 aE (dscCfaE), a stabilized form of the CFA/I fimbrial tip adhesin, is a protective antigen, using a l

282 , providing the first clinical evidence that fimbrial tip adhesins function as protective antigens.

283 e of FimH incorporated into the multiprotein fimbrial tip, where the anchoring (pilin) domain of FimH

284 The fimbrial tip-positioned adhesive protein FimH utilizes a

285 dicated that CfaE was confined to the distal fimbrial tip.

286 erved adhesive subunit, FimH, located on the fimbrial tip.

287 n or adhesin gene copies, implying that each fimbrial type was acquired by ETEC strains very recently

288 Within each fimbrial type, there were 17 non-synonymous and 1 synony

289 and those with high (8.3 +/- 1.3) numbers of fimbrial types (means +/- standard errors of the means).

290 nt and biofilm formation in vitro, and these fimbrial types are suspected to be important virulence f

291 under some conditions, indicating that both fimbrial types have unique roles in the attachment and p

292 The number of fimbrial types per E. coli isolate was distributed bimod

293 experiments, we were able to show that both fimbrial types serve to enhance colonization and persist

294 the prevalence of these 12 and 3 additional fimbrial types was determined for a collection of 303 E.

295 ological and biochemical differences between fimbrial types, regardless of class, provide structural

296 Between the fimbrial types, the pairwise nucleotide diversity for th

297 Recently, we identified a fimbrial usher gene in uropathogenic Escherichia coli st

298 sis of the CFT073 genome indicates that this fimbrial usher gene is part of a novel fimbrial gene clu

299 Each K88 fimbrial variant shows a unique pattern in binding to di

300 ossess a horizontally acquired Yersinia-like fimbrial (YLF) gene cluster.