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1 st predators as well as specialist predatory fireflies.
2 f biological oscillators such as neurons and fireflies.
3 to explain the efficient bioluminescence of fireflies.
5 lication of both viruses, generated suitable firefly and Renilla luciferase reporter constructs, resp
7 t-regulated shRNA transgenic lines targeting Firefly and Renilla luciferases, Oct4 and tumor suppress
8 PDAC cells genetically engineered to express firefly- and Gaussia luciferase, simplifying the ability
9 Many key advances in our understanding of firefly biology and signaling have been made over the pa
10 en as experimental support for mechanisms of firefly bioluminescence color that require only a single
12 the physics underlying the varied colors of firefly bioluminescence remains elusive because it is di
13 l-1,2-dioxetanone, a more adequate model for firefly bioluminescence, and found a singlet quantum yie
14 ial for only one of the partial reactions of firefly bioluminescence, supporting the proposal that th
17 animal model to determine the ability of the FireFly camera system to detect fluorescent SLNs after a
18 s, were dissected with the assistance of the FireFly camera system, a fluorescence-capable endoscopic
21 bservation of selective DYRK1A inhibition by firefly d-luciferin, we have explored static and dynamic
23 ond time-resolved spectroscopic study of the firefly emitter oxyluciferin and two of its chemically m
24 hin the family Lampyridae, new insights into firefly flash control, and the discovery of firefly nupt
25 This review provides new insight into how firefly flash signals have been shaped by the dual evolu
26 excited-state keto-enol tautomerization of a firefly fluorophore, and it could be important in resolv
29 knockin mouse were genetically modified with firefly luciferase (APL(luc)) to allow tracking by biolu
30 the bioluminescent fusion reporters beta-cat firefly luciferase (beta-cat-FLuc) and beta-cat click be
31 rine CT26 colorectal cancer cells expressing firefly luciferase (CT26-Luc), and the ACE-CD vector was
33 yc activity in living subjects using a split Firefly luciferase (FL) complementation strategy to dete
34 etween the three luciferases [mtfl, tfl, and firefly luciferase (fl)] both in cell culture and in liv
35 s (SkMb), and fibroblasts (Fibro) expressing firefly luciferase (Fluc) and green fluorescence protein
36 very frequently use reporter enzymes such as firefly luciferase (FLuc) as indicators of target activi
37 g of mice infected with parasites expressing firefly luciferase (FLUC) driven by the SAG2D promoter,
38 noninvasively, we developed a chimeric EGFR-firefly luciferase (FLuc) fusion reporter to directly mo
42 m cells (gADSCs) were transduced with either Firefly luciferase (Fluc) or Gaussia luciferase (Gluc) r
43 la luciferase (RLuc), beta-galactosidase and firefly luciferase (FLuc) ORFs linked in frame and separ
46 LK1 confers MAPK specificity by activating a firefly luciferase (FLuc) reporter gene when the Ets-lik
47 its activity to an off-target effect on the Firefly luciferase (FLuc) reporter used in the developme
48 mouse model that constitutively expresses a firefly luciferase (FLuc) split reporter complementation
50 or VEEV (subtype IC strain 3908) expressing firefly luciferase (fLUC) to simulate mosquito infection
51 nsgenic reporter mouse strain that expresses firefly luciferase (Fluc) under the regulatory control o
52 imaging reporter mice in which expression of firefly luciferase (FLuc) was placed under the control o
53 to synthesize d-luciferin, the substrate for firefly luciferase (Fluc), along with a novel set of ele
55 r finding that d-luciferin, the substrate of firefly luciferase (fLuc), is a specific substrate of AB
56 tation biosensor based on optical imaging of Firefly luciferase (FLuc), to quantitatively image p53 s
59 ic promoter virus expressing either a large, firefly luciferase (fLuc; 1,650 nucleotides), or small,
61 stably express green fluorescent protein and firefly luciferase (GFP(+)/Luc(+)) were used for the tra
64 ct organisms, we engineered an IkappaB alpha-firefly luciferase (IkappaB alpha-FLuc) fusion reporter.
65 ismate synthase (ICS1) promoter was fused to firefly luciferase (luc) and a homozygous transgenic lin
67 second construct was generated in which the firefly luciferase (Luc) gene was inserted in place of H
68 the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV
69 d in transgenic mice containing an inducible firefly luciferase (luc) reporter gene under transcripti
71 ter mouse model that predominantly expresses firefly luciferase (luc2p) in the paw epidermis--the reg
72 st created a mutant (mtfl) of a thermostable firefly luciferase (tfl) bearing the peroxisome localiza
73 simplex virus type I thymidine kinase [tk], firefly luciferase [fl], and Renilla luciferase [rl]) pl
75 elongation errors by monitoring the level of firefly luciferase activity from a mutant allele inactiv
79 en complementary non-functional fragments of firefly luciferase allows direct detection of IP(3) in p
80 izing two different split-protein reporters, firefly luciferase and beta-lactamase, while also testin
82 inhibitor of metalloproteinases 3 (TIMP3) or firefly luciferase and designated them rQT3 and rQLuc, r
84 background) that constitutively express both firefly luciferase and enhanced green fluorescence prote
85 ng double-fusion reporter gene consisting of firefly luciferase and enhanced green fluorescent protei
86 asts were lentivirally transduced to express firefly luciferase and green fluorescent protein (GFP).
87 ts were genetically modified to express both firefly luciferase and green fluorescent protein (mH9c2)
88 FVB mice with a beta-actin promoter driving firefly luciferase and green fluorescent protein double
89 by sectioning mouse hearts (n=40) expressing firefly luciferase and green fluorescent protein into sl
90 ell line transduced with a vector expressing firefly luciferase and green fluorescent protein were tr
92 sion (TF; monomeric red fluorescent protein, firefly luciferase and herpes simplex virus thymidine ki
93 lar bioenergetics and the signal obtained by firefly luciferase and human sodium-iodide symporter lab
96 ransduced with an ubiquitin promoter driving firefly luciferase and monomeric red fluorescence protei
97 ally brighter signals from deep tissues than firefly luciferase and other bioluminescent proteins.
99 attached to the C-terminal fragment of split-firefly luciferase and the coiled-coil Fos, which is spe
100 i.v. administration of Ad vectors expressing firefly luciferase and to retarget virus to hepatic colo
102 ect effect on the FLuc reporter, implicating firefly luciferase as a molecular target of PTC124.
104 introduction of green fluorescent protein or firefly luciferase as fusions with replicase proteins.
105 has Renilla luciferase as the 5' cistron and firefly luciferase as the 3' cistron, has been found to
108 l BRET-FRET energy transfer process based on firefly luciferase bioluminescence can be employed to as
110 e molecules are hydrolyzed to substrates for firefly luciferase by the enzyme fatty acid amide hydrol
115 new crystal structures of the product-bound firefly luciferase combined with the previously determin
119 Analogues of dihydrofolate reductase and firefly luciferase containing glycosylated amino acids a
122 elii YM strain (PyLuc) that stably expresses firefly luciferase driven by a constitutive promoter.
123 gment complementation associated recovery of firefly luciferase enzyme activity with intact firefly l
126 harge-coupled device camera, the analysis of firefly luciferase fragment complementation in transient
127 mbinations of nonoverlapping and overlapping firefly luciferase fragments that can be used for studyi
128 ic islets (200 per recipient) expressing the firefly luciferase from FVB/NJ strain (H-2q) mice were t
129 binant CII proteins more efficiently protect firefly luciferase from insolubilization during heating
131 sses a bioluminescent reporter consisting of firefly luciferase fused to a region of HIF that is suff
133 novel transcriptionally coupled IkappaBalpha-firefly luciferase fusion reporter and characterized the
135 anking region was inserted upstream from the firefly luciferase gene and the chimeric construct was t
137 We identified different fragments of the firefly luciferase gene based on the crystal structure o
138 abidopsis thaliana (Col-0) line containing a firefly luciferase gene controlled by a promoter region
139 herpesvirus 68) was constructed to express a firefly luciferase gene driven by the viral M3 promoter
140 veloped a transgenic mouse model wherein the firefly luciferase gene expression was dependent on the
141 -based methodology for rapidly integrating a firefly luciferase gene in somatic cells under the contr
142 this end, the APP1 promoter was fused to the firefly luciferase gene in the C. neofor-mans GAL7:IPC1
143 transfected with a reporter comprised of the firefly luciferase gene interrupted by an abnormally spl
144 explants from transgenic mice containing the firefly luciferase gene luc controlled by the mPer1 prom
146 g the IFN-stimulated gene 56 promoter-driven firefly luciferase gene stably integrated in a TLR3-expr
147 nstructed a di-cistronic reporter in which a firefly luciferase gene was linked to a chloramphenicol
148 ation of an adenovirus vector containing the firefly luciferase gene was measured serially and noninv
149 ression of the coding region of the enhanced firefly luciferase gene were employed to identify region
151 c and Cfluc are the N and C fragments of the firefly luciferase gene, respectively): Nfluc (1-475)/Cf
155 ic region was cloned between the Renilla and firefly luciferase genes, which acted as reporters of OR
158 translation of a downstream cistron encoding Firefly luciferase in a dicistronic expression vector.
160 to achieve specific and robust expression of firefly luciferase in the prostate glands of transgenic
166 by using dual-luciferase reporters, in which firefly luciferase is used as the retrotransposition ind
167 zacytidine treatment led to higher levels of firefly luciferase mRNA (RT-PCR) and protein (Western bl
168 and resolubilization of thermally denatured firefly luciferase occurred independently of NEF activit
169 n-replicating mammalian construct within the firefly luciferase open reading frame, or at the 5' or 3
170 Delivery studies with pDNA containing the firefly luciferase or beta-galactosidase reporter genes
171 n HIV-1-derived lentiviral vector expressing firefly luciferase permitting the use of bioluminescence
173 erization state of each mutant using a split firefly luciferase protein fragment-assisted complementa
174 ansfected with nonoxidized mRNA encoding the firefly luciferase protein were cultured in the presence
175 Plasmodium falciparum strain expressing the firefly luciferase protein, we present a luminescence-ba
177 Hence, we constructed biotinylated fused firefly luciferase proteins, immobilized the proteins on
178 on proteins that could lead to split Renilla/firefly luciferase reporter complementation in the prese
179 ds carrying cytomegalovirus promoter driving firefly luciferase reporter gene (CMV-Fluc) and passaged
180 oying parasites with an integrated copy of a firefly luciferase reporter gene and a secondary flow cy
181 gnificantly reduce the expressions of both a firefly luciferase reporter gene and an ectopic MLH1 gen
183 ULTR1;2 promoter (2.2 kb) was fused with the firefly luciferase reporter gene to quantitatively repor
185 iana mutants for deregulated expression of a firefly luciferase reporter gene under the control of th
189 lated region and regulated the expression of firefly luciferase reporter in a dose-dependent manner.
191 ng furans situated 2, 5 or 12 bps apart in a firefly luciferase reporter plasmid caused a decrease in
193 because this factor inhibits expression of a firefly luciferase reporter under the control of the BAG
194 bioluminescence imaging using a constitutive firefly luciferase reporter, while TGFbeta signaling in
200 estern Reserve vaccinia virus that expresses firefly luciferase to infect wild-type C57BL/6 and TLR3-
201 tein fragment complementation assay based on firefly luciferase to investigate dimerization of chemok
202 nal siRNAs targeting a different site on the firefly luciferase transcript or endogenously expressed
203 A piggyBac donor plasmid modified to encode firefly luciferase under control of schistosome gene pro
204 to contain like green fluorescent protein or firefly luciferase under control of the cytomegalovirus
207 fusion proteins consisting of a thermostable firefly luciferase variant that catalyze yellow-green (5
209 As a proof-of-principle, photoregulation of firefly luciferase was achieved in live cells by caging
210 ured fibroblasts, in which the expression of firefly luciferase was driven by the promoter of the cir
211 refly luciferase enzyme activity with intact firefly luciferase was estimated for different fragment
215 nd carboxy-fragment (395-550 amino acids) of firefly luciferase were fused to amino-terminal of Apaf-
217 n (FVB/NJ-luc) that constitutively expressed firefly luciferase were transplanted to various implanta
218 the fusion of green fluorescent protein and firefly luciferase with either nonstructural protein 2 o
219 strategy to identify a novel split site for firefly luciferase with improved characteristics over pr
220 rter mouse line that pairs the expression of firefly luciferase with quantifiable expression of a hum
221 vity in HeLa cells of siRNA duplexes against firefly luciferase with substitutions in the guide stran
222 colons of mice administered an mRNA encoding firefly luciferase with ultrasound and the D-luciferin s
223 (DF; enhanced green fluorescent protein and firefly luciferase) or triple fusion (TF; monomeric red
224 ATP as substrate, pyruvate kinase (PK), and firefly luciferase) to generate ATP, with measurement of
225 ontaining monomeric red fluorescent protein, firefly luciferase, and herpes simplex virus thymidine k
226 nterfering RNA (siRNA) against a model gene, firefly luciferase, and PEI25 or PEI87 afforded a 77% an
227 sHSPs with three different model substrates, firefly luciferase, citrate synthase, and malate dehydro
228 talysts (cysteamine) was used to encapsulate firefly luciferase, green and blue fluorescent proteins
229 hat D-luciferin, the endogenous substrate of firefly luciferase, is a specific substrate for ABCG2.
230 e-fusion (TF) reporter gene that consists of firefly luciferase, monomeric red fluorescence protein,
231 ple-fusion reporter, fluc-mrfp-ttk (encoding firefly luciferase, monomeric red fluorescent protein, a
232 ate-forming enzyme superfamily that includes firefly luciferase, nonribosomal peptide synthetases, an
233 on-ribosomal peptide synthetases (NRPS), and firefly luciferase, perform two half-reactions in a ping
234 cules from the murine Hmox1 locus, including firefly luciferase, to allow long-term, non-invasive ima
235 nto the cytoplasm, measured by expression of firefly luciferase, was increased more than 10-fold by e
236 encapsulation of horseradish peroxidase and firefly luciferase, we demonstrate that this new protoco
237 transgenic T. cruzi Y luc strain expressing firefly luciferase, we prioritized the biaryl and N-aryl
239 ord chemiluminescence (CL) from an optimized firefly luciferase-ATP bioluminescence reaction system,
243 on henipavirus pathogenesis, we generated a firefly luciferase-expressing NiV and monitored virus re
244 selective accumulation of i.v. administered firefly luciferase-expressing T cells in intracerebral x
262 siRNA in skin was tested by co-delivering a firefly luciferase/mutant K6a bicistronic reporter const
263 ntiviral vectors encoding the reporter genes firefly-luciferase and murine interleukin-10 were admini
264 trate that both the engineered and wild-type firefly luciferases tolerate much greater steric bulk at
265 f the C-terminal domain and conserved in all firefly luciferases, are each essential for only one of
267 They share 19 to 21% sequence identity with firefly luciferases, which produce light using ATP and t
268 we reported that the synthetic adenylate of firefly luciferin analogue D-5,5-dimethylluciferin was t
270 ng animals that employs in situ formation of firefly luciferin from two complementary caged precursor
271 Once released, HCBT and D-cysteine form firefly luciferin in situ, giving rise to a bioluminesce
274 selectively reacts with H(2)O(2) to release firefly luciferin, which triggers a bioluminescent respo
281 he steady-state and time-resolved spectra of firefly oxyluciferin complexed with luciferase from the
282 pectroscopy to compare the absorption by the firefly oxyluciferin lumophore isolated in vacuo and com
291 he ATP-dependent luciferase derived from the firefly Photuris pennsylvanica that was optimized using
292 system to examine the expression profiles of firefly reporter mRNAs bearing alternative combinations
293 we developed a dual-luciferase (Renilla and Firefly) reporter system for high-throughput screening (
294 luciferases obtained from beetle, including fireflies, result in novel properties and offer opportun
297 tion between S1P1 and beta-arrestin2 via the firefly split luciferase fragment complementation system
299 lampyrids, including evidence from Photinus fireflies that females choose their mates on the basis o
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