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1 ated to promote symbiotic colonization by V. fischeri.
2 rum sensing proteins LuxR and LuxI of Vibrio fischeri.
3 n OMV production and biofilm formation by V. fischeri.
4 a scolopes and the luminous bacterium Vibrio fischeri.
5 opes and the bioluminescent bacterium Vibrio fischeri.
6 a second anthranilate-activating NRPS in N. fischeri.
7 rahaemolyticus, Vibrio vulnificus and Vibrio fischeri.
8 milar to that triggered by infection with V. fischeri.
9 ted to RscS-mediated biofilm formation in V. fischeri.
10 tes the aphrodisiac-like activity of live V. fischeri.
11 ironment to regulate biofilm formation by V. fischeri.
12 e loci contribute to biofilm formation in V. fischeri.
13 omonas aeruginosa; the model symbiont Vibrio fischeri.
14 on the acute toxicity of OSPW toward Vibrio fischeri.
15 for two CsrA-regulating small RNAs in Vibrio fischeri.
16 clic di-GMP in the control of motility of V. fischeri.
17 quorum sensing signaling pathway from Vibrio fischeri.
18 inally studied in the marine symbiont Vibrio fischeri.
19 CRP, may help control cAMP-CRP activity in V.fischeri.
20 modulating other symbiotic functions, in V. fischeri.
21 promoting colonization of E. scolopes by V. fischeri.
22 ls relative to that achieved by wild-type V. fischeri.
23 factor similar to the LuxR protein of Vibrio fischeri.
24 high density by the marine bacterium Vibrio fischeri.
25 ts specific, bioluminescent symbiont, Vibrio fischeri.
26 Euprymna scolopes, the symbiotic host of V. fischeri.
27 and characterized a glnD:mTn5Cm mutant of V. fischeri.
28 um-sensing control of luminescence in Vibrio fischeri.
29 -HSL-responsive quorum-sensing regulon in V. fischeri.
30 s, VAI, the closely related signal of Vibrio fischeri.
31 nd the LuxR transcription factor from Vibrio fischeri.
32 vium, and a filamentous fungus, Trichophyton fischeri.
33 th the luciferases from P. leiognathi and V. fischeri.
34 an elevated bacterial toxicity to Aliivibrio fischeri.
35 positively control bioluminescence in Vibrio fischeri.
36 urring, non-bioluminescent strains of Vibrio fischeri.
40 d Eisenia fetida, the marine bacteria Vibrio fischeri, a suite of ten prokaryotic species, and a euka
42 ted intracellular citrate levels in a Vibrio fischeri aconitase mutant correlate with activation of t
43 We have investigated the impact of the V. fischeri acyl-HSL synthase AinS on both luminescence and
44 st that the two quorum-sensing systems in V. fischeri, ain and lux, sequentially induce the expressio
45 ful colonization of E. scolopes, i.e. the V. fischeri ainS mutant failed to persist in the squid ligh
48 his system include the genome sequence of V. fischeri, an expressed sequence tagged library for E. sc
51 rine lactone, freely diffuses through Vibrio fischeri and Escherichia coli cells has led to the assum
52 s important for symbiotic colonization by V. fischeri and establishes a paradigm for evaluating two-c
54 s the predominately expressed catalase in V. fischeri and indicating that V. fischeri carries only a
55 f the symbiosis between the bacterium Vibrio fischeri and its squid host, which can be observed direc
58 lifies the ecological differences between T. fischeri and T. rubrum and illustrates how correct ident
59 athed flagellum in both the mutualist Vibrio fischeri and the pathogen Vibrio cholerae promotes relea
60 iation between the luminous bacterium Vibrio fischeri and the sepiolid squid Euprymna scolopes provid
61 n the symbiosis between the bacterium Vibrio fischeri and the squid Euprymna scolopes, which proceeds
63 oserine lactone synthase gene luxI of Vibrio fischeri and three genes that resemble the acylhomoserin
64 uorum sensing systems such as LuxR of Vibrio fischeri and TraR of Agrobacterium tumefaciens, there is
65 otein necessary for biofilm maturation by V. fischeri and, based on the conservation of bmp, potentia
66 ction between the Lux and Syp pathways in V. fischeri, and furthers our understanding of how the Lux
67 tants of the bioluminescent bacterium Vibrio fischeri, and have demonstrated that the host squid Eupr
68 philus influenzae, Proteus mirabilis, Vibrio fischeri, and Pasteurella multocida are all cleaved by R
69 ADase activity in culture supernatants of V. fischeri, and this mutant initiated the light organ symb
70 a scolopes forms a natural symbiosis with V. fischeri, and utilizes the symbiont-derived bioluminesce
72 l regulation of TMAO reductase operons in V. fischeri appears to differ from that in previously studi
73 NADPH-utilizing flavin reductase from Vibrio fischeri are quite similar to that of the wild-type FRP(
74 This study lays the foundation for using V. fischeri as a model system for studying TMAO reductases
77 work suggests that the tissue with which V. fischeri associates not only can detect bioluminescence
84 ht organ symbiont of Euprymna spp. is Vibrio fischeri, but until now, the light organ symbionts of Se
85 ecologically distinct bioluminescent Vibrio fischeri by colonization and growth within the light org
86 strate that SypE controls biofilms in Vibrio fischeri by regulating the activity of SypA, a STAS (sul
87 popolysaccharide (LPS) or free lipid A of V. fischeri can trigger morphological changes in the juveni
89 ggregation, suggest a two-step process of V. fischeri cell engagement: association with host cilia fo
90 ctly and in real time, approximately five V. fischeri cells aggregate along the mucociliary membranes
91 rrounding mucus, the environment in which V. fischeri cells aggregate before migration into the organ
92 h inocula demonstrated that environmental V. fischeri cells aggregate during a 3 h period in host-she
94 nduced three- to fourfold both as growing V. fischeri cells approach stationary phase and upon the ad
96 -Seq dataset representing host-associated V. fischeri cells from colonized juvenile E. scolopes, as w
97 glycerol/tryptone-based medium, wild-type V. fischeri cells initially excrete acetate but, in a metab
98 s light organ symbiosis is initiated when V. fischeri cells present in the surrounding seawater enter
100 s localized in the periplasm of wild-type V. fischeri cells, where its role could be to detoxify hydr
104 a galactose-utilization mutant (galK) of V. fischeri colonized juvenile squid to wild-type levels, i
106 by SypE is a critical mechanism by which V. fischeri controls biofilm development and symbiotic colo
107 thus reveals a novel group of genes that V. fischeri controls through a sigma54-dependent response r
110 y demonstrated that oxygen consumption by V. fischeri CydAB quinol oxidase is inhibited by NO treatme
111 imitation or (ii) a mutation that renders V. fischeri defective in the synthesis of a homolog of the
114 Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-acetyl
115 t cAMP-CRP is active and important in Vibrio fischeri during colonization of its host squid Euprymna
118 rio symbiosis, the bacterial symbiont Vibrio fischeri encounters host-derived NO, which has been hypo
122 s model, we found that bioluminescence in V. fischeri ES114 is modulated by glucose and stimulated by
123 ble for this phenomenon, the lipid A from V. fischeri ES114 LPS was isolated and characterized by mul
124 t known relatives, flrA mutant strains of V. fischeri ES114 were completely abolished in swimming cap
125 vnA and HvnB are proteins secreted by Vibrio fischeri ES114, an extracellular light organ symbiont of
128 Building on the genome project of Vibrio fischeri ES114, we used a comparative approach to identi
129 Here we report the genome sequence of V. fischeri ES114, which enters into a mutualistic symbiosi
131 nescent bacteria inhibition test with Vibrio fischeri exhibited that the TPs irgarol sulfoxide and te
134 Magnesium-dependent induction of Vibrio fischeri flagellar (Mif) biogenesis depends upon two dig
136 (i) selection of the specific partner Vibrio fischeri from the bacterioplankton during symbiosis onse
138 osition in a 1 mM bulk solution above Vibrio fischeri (gamma-Protebacteria-Vibrionaceae) bacterial bi
141 l host and presents the first examples of V. fischeri genes that affect normal host tissue developmen
142 -Mbp genome sequence represents the first V. fischeri genome from an S. robusta symbiont and the firs
145 edicted, in the presence of NO, wild-type V. fischeri grew more slowly on hemin than a hnoX deletion
149 ion of the lux operon (luxICDABEG) of Vibrio fischeri has been intensively studied as a model for quo
152 the luminescence (lux) genes of symbiotic V. fischeri have been shown to be highly induced within the
155 n colonizing the light organ of the model V. fischeri host, the Hawaiian bobtail squid Euprymna scolo
156 id not compromise symbiotic competence of V. fischeri; however, levels of colonization of an ainS lux
157 reduced the inhibition of luminescence to V. fischeri, i.e., were beneficial for the bacteria, with P
159 rmed with a truncated lux operon from Vibrio fischeri, in the presence of 1-100 nM exogenous acyl-hom
160 discovered that the marine bacterium Vibrio fischeri induces sexual reproduction in one of the close
161 s, this work reveals a mechanism by which V. fischeri inhibits cellulose-dependent biofilm formation
163 lay a role in the normal process by which V. fischeri initiates its colonization of the nascent light
168 these results show that the flagellum of V. fischeri is a complex structure consisting of multiple f
169 gulation of the luminescence genes in Vibrio fischeri is a model for quorum sensing in Gram-negative
171 cence emitted by the marine bacterium Vibrio fischeri is a particularly striking result of individual
173 During the early stages of colonization, V. fischeri is exposed to host-derived nitric oxide (NO).
174 uxICDABEG) of the symbiotic bacterium Vibrio fischeri is regulated by the transcriptional activator L
175 Genetic arrangement of the flrA locus in V. fischeri is similar to motility master-regulator operons
181 fied TMAO reductase activity in symbiotic V. fischeri isolates associated with the light organs of ad
184 it is possible that these features of the V. fischeri lipid A may underlie the ability of E. scolopes
185 ild-type and waaL strains showed that the V. fischeri LPS has a single O-antigen repeat composed of y
188 , we identified three novel regulators of V. fischeri luminescence and seven regulators that altered
191 vator that controls expression of the Vibrio fischeri lux operon in response to an acylhomoserine lac
196 ein and a number of other homologs of Vibrio fischeri LuxR that function as receptors for N-acylhomos
197 Taken together, these data indicate that V. fischeri LuxS affects both luminescence regulation and c
198 this study, we investigated the impact of V. fischeri LuxS on luminescence and colonization competenc
199 e closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenic
202 new subgroup of PTase genes from Neosartorya fischeri, Microsporum canis, and Trichophyton tonsurans
204 ly identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on LuxR a
205 ablishing that there is a LuxR regulon in V. fischeri MJ-100 whose genes are coordinately expressed d
206 re fully characterize the LuxR regulon in V. fischeri MJ-100, real-time reverse transcription-PCR was
208 To understand environmental influences on V. fischeri motility, we investigated migration of this org
215 scence reactions using FRP(H) and the Vibrio fischeri NAD(P)H-utilizing flavin reductase G (FRG(F)) i
216 ganisms and toxicity end points using Vibrio fischeri (nonspecific), specific fish macrophage antimic
218 acetylaszonalenin synthetase of Neosartorya fischeri NRRL 181 activates anthranilate to anthranilyl-
219 These studies indicate that the unusual V. fischeri O-antigen sugars play a role in the early phase
220 ent with that activity, introduction into V. fischeri of medium-copy plasmids carrying these genes in
222 a scolopes and the luminous bacterium Vibrio fischeri offers the opportunity to decipher the hour-by-
231 lar Microbiology, Dunn et al. report that V. fischeri produces an NO-inducible and NO-resistant alter
232 e here that the flavohaemoglobin, Hmp, of V. fischeri protects against NO, both in culture and during
234 h a recently developed metabolic model of V. fischeri provides us with a clearer picture of the metab
238 enes, we examined the protein patterns of V. fischeri quorum-sensing mutants defective in luxI, ainS,
243 ymna scolopes by the marine bacterium Vibrio fischeri requires the symbiosis polysaccharide (syp) gen
244 ilm formation by the marine bacterium Vibrio fischeri requires the Syp polysaccharide, but the involv
245 whether the net release of PG monomers by V. fischeri resulted from lytic transglycosylase activity o
246 gions of a number of flagellar operons in V. fischeri revealed apparent sigma(54) recognition motifs,
249 describe the draft genome sequence of Vibrio fischeri SR5, a squid symbiotic isolate from Sepiola rob
250 tivity associated with whole cells of Vibrio fischeri strain ES114 was identified as the product of a
251 ation of juvenile E. scolopes; however, a V. fischeri strain lacking TMAO reductase activity displays
254 pared and analyzed the ain locus from two V. fischeri strains and a Vibrio salmonicida strain to expl
261 eriments demonstrate that the E. scolopes-V. fischeri system is a viable model for the experimental s
264 is specifically colonized by cells of Vibrio fischeri that are obtained from the ambient seawater.
265 606 bp open reading frame was cloned from V. fischeri that encoded a protein, which we named LitR, th
266 have cloned a gene, designated flrA, from V. fischeri that encodes a putative sigma(54)-dependent tra
267 o high density, we identified a mutant of V. fischeri that exhibited an apparent defect in symbiosis
269 na scolopes and its bacterial partner Vibrio fischeri, the bacteria induce dramatic morphogenesis of
272 derivatives (1-4) isolated from Neosartorya fischeri, three of which had significant antimicrobial a
274 e understanding of the matrix produced by V. fischeri to enhance cell-cell interactions and promote s
276 accharide locus, syp, is required for Vibrio fischeri to form a symbiotic association with the squid
279 his association, we searched a library of V. fischeri transposon insertion mutants for those that fai
282 a component of the host defense, and that V. fischeri uses a cytotoxin-like molecule to induce host d
286 -scale mutagenesis of a class of genes in V. fischeri using a genomic approach and emphasizes the imp
287 iofilm formation and host colonization by V. fischeri via its impact on transcription of the symbiosi
289 Complete removal of toxicity toward Vibrio fischeri was achieved after ozonation followed by 28-day
290 The acute toxicity of OSPW toward Vibrio fischeri was reduced after the solar/chlorine treatment.
291 genetic tractability of the bacterium Vibrio fischeri, we explore the regulation of aox expression an
292 sion of LitR, the global regulator in Vibrio fischeri, we have performed a series of genetic and phen
294 other regulators of biofilm formation in V. fischeri, we screened a transposon library for mutants d
295 de molecules released by the bacteria Vibrio fischeri when it rotates its flagella prompts its host,
296 have focused on the LuxR protein from Vibrio fischeri, which activates gene transcription in response
297 r the marine bioluminescent bacterium Vibrio fischeri, which exists either in a free-living state or
298 scolopes and its beneficial symbiont Vibrio fischeri, which form a highly specific binary mutualism.
299 f the bioluminescent marine bacterium Vibrio fischeri with its animal host, the Hawaiian bobtail squi
300 in a mixed population of Vibrio logei and V. fischeri, with V. logei comprising between 63 and 100% o
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