ライフサイエンスコーパス: flagellar

1 Our results demonstrate that flagellar abundance correlates with growth rate, where f

2 oteins in Caulobacter crescentus, which tune flagellar activity in response to binding of the second

3 apid and precisely timed modulation of their flagellar activity.

4 s that are coincident with specific gaits of flagellar actuation, suggesting that it is a competition

5 These findings illuminate flagellar adaptation, signal transduction cascade organi

6 We confirmed the flagellar and acidocalcisome localization of TcFCaBP and

7 Pase, FleN, FleQ regulates the expression of flagellar and exopolysaccharide biosynthesis genes in re

8 in results in increased transcription of the flagellar and the Salmonella pathogenicity island 1 (SPI

9 gellar toolkit showed a previously unnoticed flagellar apparatus in two close relatives of animals, t

10 metries of basal body positioning and of the flagellar apparatus that are coincident with specific ga

11 es were co-regulated with genes encoding the flagellar apparatus, supporting the functional contribut

12 The bacterial flagellar apparatus, which involves approximately 40 dif

13 of the type III secretion system (T3SS) and flagellar apparatus.

14 The screw-type flagellar arrangement enables a unique mode of propagati

15 pparatuses (fT3SSs), which are essential for flagellar assembly and cell motility.

16 , and their localization also changes during flagellar assembly and disassembly.

17 glycosylation plays an essential role in the flagellar assembly and motility of T. denticola.

18 d in lower expression of genes encoding many flagellar assembly components, which led to a motility d

19 unction, there is a debate as to whether the flagellar assembly function of specialized, centriolar t

20 we investigated the effect of growth rate on flagellar assembly in Escherichia coli using steady-stat

21 Numerous global regulators control flagellar assembly in response to cellular and environme

22 Previous studies have also shown that flagellar assembly is affected by the growth-rate of the

23 n small deviations from the highly regulated flagellar assembly process can abolish motility and caus

24 tentially involved in the regulation of IFT, flagellar assembly, and flagellar signaling, and provide

25 d that genes involved in bacterial mobility, flagellar assembly, bacterial chemotaxis and LPS synthes

26 Lysinoalanine crosslinks are not needed for flagellar assembly, but they are required for cell motil

27 show the T. brucei BBSome is dispensable for flagellar assembly, motility, bulk endocytosis, and cell

28 Flagellar assembly, motility, gliding, and mating are al

29 reas type B modification is not required for flagellar assembly, some mutations that result in trunca

30 . brucei polyprolyl proteins are involved in flagellar assembly.

31 order to maintain the function of the entire flagellar assembly.

32 verely decreased IFT injection frequency and flagellar-assembly defects.

33 free state and in complex with FliD and the flagellar ATPase FliI.

34 otein located within the flagellum along the flagellar attachment zone (FAZ).

35 ity and interdoublet shear stiffness, of the flagellar axoneme in the unicellular alga Chlamydomonas

36 KHARON is located at the base of the flagellar axoneme, where it likely mediates targeting of

37 bcellular distribution between cytoplasm and flagellar basal bodies, suggesting that FlhG effects fla

38 he observation of the maturation of a second flagellar basal body in late G1 phase, DNA replication i

39 r motor protein, which is located around the flagellar basal body.

40 te-specific novel component is linked to the flagellar basal body; however, nothing is known about th

41 sweeps varying the sperm head morphology and flagellar beat pattern wavenumber are conducted and reve

42 3D reconstruction algorithm to identify the flagellar beat patterns causing left or right turning, w

43 xperiments with mathematical modeling and 3D flagellar beat reconstruction to quantify the response o

44 ation rapidly elevates cAMP, accelerates the flagellar beat, and, thereby, changes swimming behavior

45 g at an adaptation mechanism controlling the flagellar beat.

46 mechanical force to generate the ciliary and flagellar beat.

47 dyneins generating the force for ciliary and flagellar beating essential to movement of extracellular

48 nce between the ATP consumption rate and the flagellar beating frequency, with approximately 2.3 x 10

49 y more common model systems, and the complex flagellar beating shapes that power it make its quantita

50 Moreover, we demonstrate that flagellar behavior depends strongly on the assumptions o

51 output - precise numerical control of polar flagellar biogenesis required to create species-specific

52 ates must spatially and numerically regulate flagellar biogenesis to create flagellation patterns for

53 Flagellar biogenesis was observed to exert temporal cont

54 FlhG ATPases numerically regulate polar flagellar biogenesis, yet FlhG orthologs are diverse in

55 logs are involved in numerical regulation of flagellar biogenesis.

56 Flagellar biosynthesis is underpinned by a specialized t

57 with a reduced level of the 32-gene fla/che flagellar biosynthesis operon transcript.fla/che operon

58 pecifically degrades the master regulator of flagellar biosynthesis SwrA governed by the adaptor prot

59 FlhE regulates an important aspect of proper flagellar biosynthesis.

60 he appropriate spatial and temporal point in flagellar biosynthesis.

61 ic pathways and a suppression of ciliary and flagellar biosynthetic pathways.

62 tipartite mechanism that likely influences a flagellar biosynthetic step to control flagellar number

63 Scattering data from aligned flagellar bundles confirmed the theoretically predicated

64 he ability to steer these devices and induce flagellar bundling in multi-flagellated nanoswimmers.

65 It interacts with a complex of the flagellar C-ring proteins FliM and FliY (also FliN) in t

66 teins of known localization such as TcFCaBP (flagellar calcium binding protein) and TcVP1 (vacuolar p

67 The flagellar capping protein (FliD) regulates filament asse

68 f these spirochetes depends crucially on the flagellar/cell wall stiffness ratio.

69 FliT is a key flagellar chaperone that binds to several flagellar prot

70 for FliT appear to be shared among the other flagellar chaperones.

71 d to microtubule function besides regulating flagellar/cilia motility.

72 ans, exposed on the PF surface, and named it Flagellar-coiling protein A (FcpA).

73 sis of the travel time of IFT protein in the flagellar compartment.

74 the assignment of the locations of the major flagellar components, and provide crucial constraints fo

75 ncy to form multimers independently of other flagellar components.

76 g mispositioning of the kinetoplast, loss of flagellar connection, and prevention of cytokinesis.

77 at ultimately determines the precise form of flagellar coordination in unicellular algae.

78 ns of the regulator CheY-P are the source of flagellar correlations.

79 rnal to the cell, must provide an additional flagellar coupling.

80 the cell cycle and its centriole-basal body-flagellar cycle.

81 ural knowledge is critical before evaluating flagellar defects.

82 SwrA protein levels to increase and elevate flagellar density above a critical threshold for swarmin

83 about the role of protein methylation during flagellar dynamics, we focused on protein arginine methy

84 To better understand the function of flagellar ectosomes, we have compared their protein comp

85 lagellum-flagellum cross-linking, as well as flagellar entanglement with bacterial bodies, suggesting

86 ximal flagellum inflexible and alters the 3D flagellar envelope, thus preventing sperm from reorienti

87 The membrane-embedded part of the flagellar export apparatus contains five essential prote

88 ed that disrupting certain components of the flagellar export apparatus inhibited transcription of th

89 coexist in a complex in both cytoplasmic and flagellar extracts.

90 e addressed a significant question whether a flagellar filament can form a new cap and resume growth

91 We can now show that the archaeal flagellar filament contains a beta-sandwich, previously

92 show that, when a cell gets stuck, the polar flagellar filament executes a polymorphic change into a

93 The bacterial flagellar filament has long been studied to understand h

94 A-dependent repression of translation of the flagellar filament protein, flagellin.

95 ximately 13 different proteins with a single flagellar filament protein.

96 The bacterial flagellar filament, largely composed of a single protein

97 In contrast to the bacterial flagellar filament, where the outer globular domains mak

98 otein that forms the anchor for the archaeal flagellar filament.

99 he chief flagellar protein flagellin and the flagellar filament.

100 Flagellar filaments are assembled from thousands of subu

101 ce has been "tuned" over evolution.Bacterial flagellar filaments are composed almost entirely of a si

102 Flagellar filaments are therefore able to re-grow if bro

103 ds, we investigated the structure of SJW1660 flagellar filaments as well as the intermolecular forces

104 ella putrefaciens with fluorescently labeled flagellar filaments at an agarose-glass interface.

105 l mobility is powered by rotation of helical flagellar filaments driven by rotary motors.

106 ear-atomic resolution cryo-EM structures for flagellar filaments from both Gram-positive Bacillus sub

107 Seven mutant flagellar filaments in B. subtilis and two in P. aerugin

108 acking microscope so that we could visualize flagellar filaments of tracked cells by fluorescence.

109 atomic resolution cryo-EM structures of nine flagellar filaments, and begin to shed light on the mole

110 ated with bundling of straight semi-flexible flagellar filaments.

111 in propulsion before and after the change in flagellar form.

112 n of FliI residues 401 to 410 resulted in no flagellar formation although this FliI deletion mutant r

113 its positive effect on T3SS gene expression, flagellar formation and amylovoran production.

114 s response by studying the swimming of three flagellar forms.

115 The injectisome (iT3SS) and flagellar (fT3SS) type III secretion systems are 2 virul

116 known as PCDP1, is required for ciliary and flagellar function in mice and Chlamydomonas reinhardtii

117 t-gated ion channels that, via regulation of flagellar function, enable single-celled motile algae to

118 lasmic domains were somewhat dispensable for flagellar gene regulation and assembly, suggesting that

119 bled from over 20 structural components, and flagellar gene regulation is morphogenetically coupled t

120 ation, rather than suppressing activators of flagellar gene transcription as in Vibrio and Pseudomona

121 ational fidelity downregulates expression of flagellar genes and suppresses bacterial motility.

122 In addition, deletion of flagellar genes motA and motB and chemotaxis gene cheA s

123 We examine two large clusters (ribosomal and flagellar genes) in detail.

124 the expression of type I fimbriae as well as flagellar genes, has also been implicated in this proces

125 dependent peptide motifs were represented in flagellar genes.

126 ion of H-NS and RpoS, both of which regulate flagellar genes.

127 r a given body geometry, there is a specific flagellar geometry that minimizes the critical flexibili

128 ibe the structural characterization of novel flagellar glycans from a number of hypervirulent strains

129 s our understanding of the genes involved in flagellar glycosylation and their biological roles in em

130 lastic bonds that form and break between the flagellar head and the surface are accounted for.

131 iptional regulatory circuits controlling the flagellar hierarchy and biofilm formation.

132 t the Spi-1 injectisome, like the Salmonella flagellar hook, uses a secreted molecular ruler, InvJ, t

133 rongly induces expression of fliK encoding a flagellar hook-length control protein.

134 A and IFT-B at the base of the flagellum and flagellar import of IFT-A.

135 d models presented here to measure cilia and flagellar length can be generalized to measure any membr

136 negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.

137 Maintenance of flagellar length requires an active transport process kn

138 Maintenance of flagellar length requires an active transport process kn

139 components to basal bodies is a function of flagellar length, with increased recruitment in rapidly

140 explain the effect of the pf15 mutations on flagellar length.

141 enerate a nearly complete atomic model for a flagellar-like filament of the archaeon Ignicoccus hospi

142 ry activity that is particularly abundant in flagellar lipids of Chlamydomonas reinhardtii, resulting

143 This flagellar-load based mechanism ensures that cells in the

144 r basal bodies, suggesting that FlhG effects flagellar location and number during assembly of the C-r

145 target gene promoters, the promoters of the flagellar master regulator flhDC and mrp itself, appears

146 t connects the sRNA, c-di-GMP signalling and flagellar master regulator FlhDC.

147 control occurs through the expression of the flagellar master regulator, FlhD4C2.

148 n a passive intercellular role of TFP during flagellar-mediated swarming of P. aeruginosa that does n

149 membranous nanotubes that originate from the flagellar membrane and disassociate into free extracellu

150 red their protein composition to that of the flagellar membrane from which they are derived.

151 oneme, where it likely mediates targeting of flagellar membrane proteins, but is also on the subpelli

152 n composition, being enriched in a subset of flagellar membrane proteins, proteases, proteins from th

153 ostained TbHrg indicated localization to the flagellar membrane, and scanning electron microscopy rev

154 ly traffic specific membrane proteins to the flagellar membrane, but the mechanisms for this traffick

155 failure of the calcium channel to enter the flagellar membrane, detachment of the flagellum from the

156 rter, LmxGT1, is selectively targeted to the flagellar membrane, suggesting a possible sensory role t

157 nel and is required for its targeting to the flagellar membrane.

158 hey can also resort to a radically different flagellar mode, which we discovered here.

159 nism for the study of the earliest events in flagellar morphogenesis and the type III secretion syste

160 robust regulatory mechanisms to ensure that flagellar morphogenesis follows a defined path, with eac

161 ced by a reduction in secretory activity and flagellar motility and an increase in adenosine triphosp

162 indicated that GM-CSF induced the genes for flagellar motility and pyocin production in the persiste

163 gen Clostridium difficile, c-di-GMP inhibits flagellar motility and toxin production and promotes pil

164 Our study of dimeric NDK5 in a flagellar motility control complex, the radial spoke (RS

165 us and functions in a complex that regulates flagellar motility in a calcium-dependent manner.

166 Results of mutant studies indicate that flagellar motility is involved in the observed preferenc

167 simple means to prevent steric hindrance of flagellar motility or to ensure that phage-mediated gene

168 complex (N-DRC), a key regulator of ciliary/flagellar motility that is conserved from algae to human

169 RC), which is a major hub for the control of flagellar motility, contains at least 11 different subun

170 DRC mutants, the drc3 mutant has a defect in flagellar motility.

171 entral role in the regulation of ciliary and flagellar motility.

172 axoneme, plays a central role in ciliary and flagellar motility; but, its contribution to adaptive im

173 The bacterial flagellar motor (BFM) is responsible for driving bacteri

174 The bacterial flagellar motor (BFM) is the rotary motor that rotates e

175 d by force) drives mechanosensitivity of the flagellar motor complex.

176 Recent experiments on the bacterial flagellar motor have shown that the structure of this na

177 The flagellar motor is a sophisticated rotary machine facili

178 The bacterial flagellar motor is an intricate nanomachine which conver

179 The stator-complex in the bacterial flagellar motor is responsible for surface-sensing.

180 Direction switching in the flagellar motor of Escherichia coli is under the control

181 We found that BB0286 (FlbB) is a novel flagellar motor protein, which is located around the fla

182 ns with either the CheA chemotaxis kinase or flagellar motor proteins.

183 This dissemination modality suggests that flagellar motor rotation and, by extension, motility are

184 Together, our data demonstrate that while flagellar motor rotation is necessary for spirochetal mo

185 g a non-Poissonian regulation scheme for its flagellar motor switches.

186 rotein-protein binding and single cell-based flagellar motor switching analyses.

187 swimming intervals, but the responses of the flagellar motor to the output of the chemotaxis network,

188 localizes to the poles independently of the flagellar motor, CheA, and all typical chemotaxis protei

189 on for several fundamental properties of the flagellar motor, including torque-speed and speed-ion mo

190 powered by protonmotive force: the bacterial flagellar motor, the Fo ATP synthase, and the gliding mo

191 not bind to PG until it is inserted into the flagellar motor.

192 tal of between 7 and 11 stator units in each flagellar motor.

193 ctionally equivalent bacterial flagellum and flagellar motor.

194 om the requirements of self-assembly both of flagellar motors and of chemoreceptor arrays.

195 motes switching between rotational states in flagellar motors of the bacterium Escherichia coli.

196 Although it is known that diverse bacterial flagellar motors produce different torques, the mechanis

197 We propose that higher viscous loads on flagellar motors result in lower DegU-P levels through a

198 ata strongly indicate that the S. oneidensis flagellar motors simultaneously use H(+) and Na(+) drive

199 averaging to determine in situ structures of flagellar motors that produce different torques, from Ca

200 look at the response of individual bacterial flagellar motors under stepwise changes in external osmo

201 ad wild type numbers of basal bodies and the flagellar motors were functional.

202 kinase-control domain to regulate the cell's flagellar motors.

203 n a relatively unexplored type of eukaryotic flagellar movement.

204 gnetic actuation of self-assembled bacterial flagellar nanorobotic swimmers.

205 ous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ.

206 ces a flagellar biosynthetic step to control flagellar number for amphitrichous flagellation, rather

207 G ATPase domain was not required to regulate flagellar number in C. jejuni.

208 ity, which may mechanistically contribute to flagellar number regulation.

209 ct in a complex, but we recently showed that flagellar ODA8 does not copurify with ODA5 or ODA10.

210 ion of other genes, including chemotaxis and flagellar operons, iron-regulated genes, and genes of un

211 lagellum exports both proteins that form the flagellar organelle for swimming motility and colonizati

212 affecting the N-DRC, drc3 does not suppress flagellar paralysis caused by loss of radial spokes.

213 lar sterol enrichment results from selective flagellar partitioning of specific sterol species or fro

214 The flagellar pocket (FP) is the exclusive site of uptake fr

215 lathrin and is localized to membranes of the flagellar pocket and adjacent cytoplasmic vesicles.

216 of trypomastigote cells, coincident with the flagellar pocket and Golgi apparatus.

217 surface attachment by the flagellum and the flagellar pocket, a Leishmania-like flagellum attachment

218 d more intense TbHrg accumulation toward the flagellar pocket.

219 ceptor-mediated endocytosis occurring in the flagellar pocket.

220 flagellin glycan chain and demonstrate that flagellar post-translational modification affects motili

221 bilization through the thrust force of their flagellar propulsion.

222 SSs) are evolutionarily related to bacterial flagellar protein export apparatuses (fT3SSs), which are

223 e question of size when applied to the chief flagellar protein flagellin and the flagellar filament.

224 ked to phosphorylation of an uncharacterised flagellar protein.

225 Several flagellar proteins are methylated on arginine residues d

226 ts unexpected structural similarity to other flagellar proteins at the domain level, adopts a unique

227 ey flagellar chaperone that binds to several flagellar proteins in the cytoplasm, including its cogna

228 eriments in cells that lack either all other flagellar proteins or just the MS-ring protein FliF.

229 Trypanosome EVs contain several flagellar proteins that contribute to virulence, and Try

230 tion and instead demands a minimal subset of flagellar proteins that includes the FliF/FliG basal bod

231 ing aggregation or undesired interactions of flagellar proteins, including their targeting to the exp

232 rmine the detailed location of components in flagellar radial spokes-a complex of proteins that conne

233 l localization of these PRMTs changes during flagellar regeneration and resorption.

234 lic diguanylate monophosphate (c-di-GMP) and flagellar regulator have been reported to affect the reg

235 en uptake and toward its novel function as a flagellar regulator.

236 on of the major virulence gene cagA with the flagellar regulatory circuit, essential for colonization

237 tructure of the central AAA(+) domain of the flagellar regulatory protein FlrC (FlrC(C)), a bEBP that

238 osome release increased when cells underwent flagellar resorption.

239 s are methylated on arginine residues during flagellar resorption; however, the function is not under

240 signalling state to elicit counter-clockwise flagellar responses.

241 ate flagellum-driven motility by suppressing flagellar reversal rates in a manner independent of visc

242 Cryoelectron tomography revealed that the flagellar ribbons are distorted in the mutant cells, ind

243 The flagellar rod acts as a driveshaft to transmit torque fr

244 Physiological properties of the flagellar rotary motor have been taken to indicate a tig

245 large, comparable to those generated by the flagellar rotary motor.

246 the complex that regulates the direction of flagellar rotation assume either 34 or 25 copies of th

247 rane potential which is required to energize flagellar rotation, accompanied by a decreased flagellum

248 ossesses two different stator units to drive flagellar rotation, the Na(+) -dependent PomAB stator an

249 ton-driven conformational changes that drive flagellar rotation.

250 or ATP synthesis, transport of nutrients and flagellar rotation.

251 l response regulator, CheY, and modulate the flagellar rotational direction.

252 ved chemotaxis proteins to phosphorylate the flagellar rotational response regulator, CheY, and modul

253 tors are disengaged and sequestered from the flagellar rotor when bound by MotI.

254 Sequence analysis of T3SS and flagellar ruler proteins shows that this mechanism is pr

255 howed that EB1-FP is highly mobile along the flagellar shaft and displays a markedly reduced mobility

256 onstruction of the swimming trajectories and flagellar shapes of specimens of Euglena gracilis We ach

257 We further present a new role for the flagellar sheath in triggering, rather than circumventin

258 ng protein 1 (EB1) remains at the tip during flagellar shortening and in the absence of intraflagella

259 and VPS4, attenuated ectosome release during flagellar shortening and shortening was slowed.

260 diate ectosome release and thereby influence flagellar shortening in Chlamydomonas.

261 that the PbifA promoter is dependent on the flagellar sigma factor FliA, and positively regulated by

262 e regulation of IFT, flagellar assembly, and flagellar signaling, and provide insight into the role o

263 t opisthokont lineages secondarily underwent flagellar simplification.

264 We propose a model in which flagellar stators are disengaged and sequestered from th

265 rther, these agents have opposite effects on flagellar sterol enrichment and cell metabolism in the t

266 We tested whether flagellar sterol enrichment results from selective flage

267 ia, spirochetes possess a unique periplasmic flagellar structure called the collar.

268 ffect on collar formation, assembly of other flagellar structures, morphology, and motility of the sp

269 demonstration of a self propelled, synthetic flagellar swimmer operating at low Reynolds number.

270 The flagellar swimming of euglenids, which are propelled by

271 eA, or the 16-residue "target region" of the flagellar switch protein, FliM, leads to easily measurab

272 conformation Cle proteins interact with the flagellar switch to control motor activity.

273 Mutations impairing flagellar synthesis are inferred to increase DegU-P, whi

274 protein FlrC (FlrC(C)), a bEBP that controls flagellar synthesis in Vibrio cholerae.

275 these domains have relatively minor roles in flagellar synthesis.

276 eria are capable of switching on and off the flagellar system by altering translational fidelity, whi

277 xplored the stator unit dynamics in the MR-1 flagellar system by using mCherry-labeled PomAB and MotA

278 The archaeal flagellar system has no homology to the bacterial one an

279 The archaeal flagellar system is simpler still, in some cases having

280 of FlhE results in a proton leak through the flagellar system, inappropriate secretion patterns, and

281 g virulence factors into host cells, and the flagellar system, secreting the polymeric filament used

282 ied model for C-ring assembly by NF-T3SS and flagellar-T3SS.

283 te that T. brucei senses heme levels via the flagellar TbHrg protein.

284 that motility arrest may be a consequence of flagellar tethering.

285 After photobleaching, the EB1 signal at the flagellar tip recovered within minutes, indicating an ex

286 ported with anterograde IFT particles to the flagellar tip, dissociates into smaller particles, and b

287 rticles dwelled for several seconds near the flagellar tip, suggesting the presence of stable EB1 bin

288 To investigate how EB1 accumulates at the flagellar tip, we used in vivo imaging of fluorescent pr

289 isplays a markedly reduced mobility near the flagellar tip.

290 Comparative analyses of the flagellar toolkit showed a previously unnoticed flagella

291 Flagellar tracking with exquisite precision reveals wave

292 ludes the FliF/FliG basal body proteins, the flagellar type III export apparatus components FliO, Fli

293 A and FlhB are established components of the flagellar type III secretion system.

294 Flagellar type III secretion systems (T3SS) contain an e

295 fferent filtration mechanism that requires a flagellar vane (sheet), something notoriously difficult

296 A CFD model with a flagellar vane correctly predicts the filtration rate of

297 letal assembly and remodeling, essential for flagellar wave frequency and amplitude and forward motil

298 The flagellar waveform can be decomposed into a static compo

299 hat DRC3 interacts with dynein g to regulate flagellar waveform.

300 iefs, this 3D analysis uncovers ambidextrous flagellar waveforms and shows that the cell's turning di