ライフサイエンスコーパス: flap endonuclease

1 nces relative to replication origins and the flap endonuclease.

2 clamp loader), family B DNA polymerase, and flap endonuclease.

3 lies at the base of the archway in phage T5 flap endonuclease.

4 a supplement or backup for the Rad27/Fen1 5' flap endonuclease.

5 port that the RAG1/ RAG2 recombinase is a 3' flap endonuclease.

6 members such as FEN1, GEN1 is a monomeric 5'-flap endonuclease.

7 of the EXO1 gene, which encodes an enigmatic flap endonuclease.

8 ncodes T4 RNase H, a relative of a family of flap endonucleases.

9 EN1 is a member of the XPG/Rad2 family of 5'-flap endonucleases.

10 cific endonuclease activity similar to known flap endonucleases.

11 acts with DNA polymerase beta (Pol-beta) and flap endonuclease 1 (Fen-1) and blocks Pol-beta-directed

12 replication proteins, including the enzymes flap endonuclease 1 (FEN-1) and DNA ligase I that comple

13 ociation of condensin I with the BER factors flap endonuclease 1 (FEN-1) and DNA polymerase delta/eps

14 The interaction between flap endonuclease 1 (FEN-1) and proliferation cell nucle

15 approach has been demonstrated that utilizes flap endonuclease 1 (FEN-1) fused to the Fok1 endonuclea

16 helicase-endonuclease that participates with flap endonuclease 1 (FEN-1) in Okazaki fragment processi

17 Human flap endonuclease 1 (FEN-1) is a member of the structure

18 PCNA binds to flap endonuclease 1 (FEN-1), a structure-specific endonu

19 ases is with the structure-specific nuclease Flap Endonuclease 1 (FEN-1), an enzyme that is implicate

20 Here we show that NEIL1 interacts with flap endonuclease 1 (FEN-1), an essential component of t

21 lly bound DNA polymerase beta (Pol beta) and flap endonuclease 1 (FEN-1).

22 a protein domain of WRN which interacts with flap endonuclease 1 (FEN-1).

23 ivities of DNA polymerase delta (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I (Lig1).

24 Flap endonuclease 1 (FEN1) and Dna2 are responsible for

25 Flap endonuclease 1 (FEN1) and Dna2 endonuclease/helicas

26 Flap endonuclease 1 (Fen1) and exonuclease 1 (Exo1) have

27 Human flap endonuclease 1 (FEN1) and related structure-specifi

28 This flap structure was cleaved by flap endonuclease 1 (Fen1) and the resultant nick was li

29 onuclease 1 (APE1) physically interacts with flap endonuclease 1 (FEN1) and with proliferating cell n

30 DNA polymerase beta (pol beta) and flap endonuclease 1 (FEN1) are key players in pol beta-m

31 The Dna2 helicase/nuclease and then flap endonuclease 1 (FEN1) are proposed to act sequentia

32 Dna2 nuclease/helicase and flap endonuclease 1 (FEN1) are proposed to cleave the fl

33 Flap endonuclease 1 (FEN1) cleaved the displaced downstr

34 uted with purified enzymes demonstrated that Flap endonuclease 1 (FEN1) efficiently excises a displac

35 AMP-dRP group through its lyase activity and flap endonuclease 1 (FEN1) excises the 5'-AMP-dRP group

36 The structure-specific endonuclease flap endonuclease 1 (FEN1) has also been suggested to be

37 Flap endonuclease 1 (FEN1) is a central component of Oka

38 Flap endonuclease 1 (Fen1) is a highly conserved structu

39 Flap endonuclease 1 (FEN1) is a protein that plays a rol

40 Flap endonuclease 1 (FEN1) is a structure-specific nucle

41 Flap endonuclease 1 (FEN1) is a structure-specific nucle

42 Flap endonuclease 1 (FEN1) is a structure-specific nucle

43 5'-Flap endonuclease 1 (Fen1) is an important enzyme involv

44 We identify that the flap endonuclease 1 (FEN1) is one of the interacting pro

45 that the DNA replication and repair protein Flap endonuclease 1 (FEN1) is required for replication o

46 DNA replication and repair enzyme Flap Endonuclease 1 (FEN1) is vital for genome integrity

47 Flap endonuclease 1 (FEN1) participates in removal of RN

48 Flap endonuclease 1 (FEN1) phosphorylation is proposed t

49 Flap endonuclease 1 (FEN1) plays a crucial role in both

50 Flap Endonuclease 1 (FEN1) plays important roles both in

51 nuclease 1 (FAN1), exonuclease 1 (EXO1), and flap endonuclease 1 (FEN1) process a substrate reminisce

52 Flap endonuclease 1 (FEN1) processes Okazaki fragments i

53 Flap endonuclease 1 (FEN1) proteins, which are present i

54 nent in the maturation of Okazaki fragments, flap endonuclease 1 (FEN1) removes the 5'-flap and maint

55 In one model for this process, the flap endonuclease 1 (FEN1) removes the iRNA.

56 s in concert with alternate flap cleavage by flap endonuclease 1 (FEN1) to mediate CAG repeat expansi

57 s derived from Xenopus laevis, we found that flap endonuclease 1 (FEN1) was a factor responsible for

58 Recently, Flap endonuclease 1 (FEN1) was shown to contribute to te

59 Most flaps are cleaved by flap endonuclease 1 (FEN1) while short, and the remainin

60 Here, we demonstrate that flap endonuclease 1 (FEN1), a canonical lagging strand D

61 Human flap endonuclease 1 (FEN1), an essential DNA replication

62 Polymorphisms and somatic mutations in Flap Endonuclease 1 (FEN1), an essential enzyme involved

63 se 1 (APE1), DNA polymerase beta (pol beta), flap endonuclease 1 (FEN1), and DNA ligase I (LigI).

64 n genes involved in DNA replication, such as flap endonuclease 1 (FEN1), can cause single-stranded DN

65 p21 disrupts PCNA-directed stimulation of flap endonuclease 1 (FEN1), DNA ligase I, and DNA polyme

66 AP endonuclease, replication factor C, PCNA, flap endonuclease 1 (FEN1), DNA polymerase delta, and DN

67 Flap endonuclease 1 (Fen1), has an endonuclease activity

68 In most cases, the flap was removed by flap endonuclease 1 (FEN1), in a reaction required to re

69 Flap endonuclease 1 (FEN1), involved in the joining of O

70 d cancer avoidance of the structure-specific flap endonuclease 1 (FEN1), its cellular compartmentaliz

71 , which is removed by the structure-specific flap endonuclease 1 (FEN1), leaving a nick for ligation.

72 is via long-patch BER (LP-BER) dependent on flap endonuclease 1 (FEN1), not previously known to be p

73 and handoff of DNA-editing enzymes, such as flap endonuclease 1 (FEN1), with sliding clamps are key

74 brogated two of three nuclease activities of flap endonuclease 1 (FEN1).

75 age of the flap by Dna2 nuclease followed by flap endonuclease 1 (FEN1).

76 he primer into a flap that is cleaved off by flap endonuclease 1 (FEN1).

77 Human flap endonuclease 1 (h-FEN1) mutations have dramatic eff

78 long flap (20 nucleotides) by M. acetivorans flap endonuclease 1 (MacFEN1).

79 e with a 5'- and 3'-flap that was cleaved by flap endonuclease 1 and a 3'-5' endonuclease Mus81/Eme1,

80 o profoundly altered PCNA's interaction with Flap endonuclease 1 and DNA Ligase 1, DNA metabolism enz

81 these processes, acting in conjunction with flap endonuclease 1 and replication protein A in DNA lag

82 ses, apurinic/aprymidinic endonuclease 1 and flap endonuclease 1 and several other factors involved i

83 Treatment of extracts with flap endonuclease 1 antiserum significantly reduced MHEJ

84 Instead, flap endonuclease 1 must recognize and bind to the inter

85 We further demonstrate that hDNA2 and flap endonuclease 1 synergistically process intermediate

86 ssed by DNA polymerase beta and processed by flap endonuclease 1 with different efficiency.

87 beled the eukaryotic DNA replication protein flap endonuclease 1 with mKikGR and added it to replicat

88 of the related RAD2 family nucleases, FEN1 (Flap endonuclease 1) and EXO1 (exonuclease 1), on substr

89 In most cases, FEN1 (flap endonuclease 1) is able to efficiently cleave short

90 Until cleaved by FEN1 (flap endonuclease 1), such flaps can initiate homologous

91 provide strand displacement synthesis, human flap endonuclease 1, and human DNA ligase I.

92 This resembles the mechanism of flap endonuclease 1, consistent with cooperation of thes

93 isomer of RECQL5, RECQL5beta, with the human flap endonuclease 1, FEN1, which plays a critical role i

94 ase/endonuclease, called RAD2 homologue 1 or flap endonuclease 1, has a unique cleavage activity, dep

95 Coordinating with flap endonuclease 1, the APE1 3'-5' exonuclease activity

96 a long flap that was efficiently cleaved by flap endonuclease 1, thereby leading to repeat deletion.

97 Flap endonuclease 1, which preferentially cleaves unanne

98 g is not channeled to the subsequent enzyme, flap endonuclease 1.

99 A and provides binding sites for polymerase, flap endonuclease-1 (FEN-1) and ligase during DNA replic

100 Flap EndoNuclease-1 (FEN-1) and the processivity factor

101 replication protein A (RPA), which inhibits flap endonuclease-1 (FEN-1) but stimulates Dna2 nuclease

102 y transfer (FRET) analyses show that WRN and Flap Endonuclease-1 (FEN-1) form a complex in vivo that

103 Flap endonuclease-1 (FEN-1) is a critical enzyme for DNA

104 Human flap endonuclease-1 (FEN-1) is a member of the structure

105 Human flap endonuclease-1 (FEN-1) is a member of the structure

106 Flap endonuclease-1 (FEN-1) is a multifunctional and str

107 Human flap endonuclease-1 (FEN-1) is a structure-specific endo

108 Mammalian flap endonuclease-1 (FEN-1) is a structure-specific meta

109 Flap endonuclease-1 (FEN-1) is a structure-specific nucl

110 termediates in DNA replication and repair by flap endonuclease-1 (FEN-1) is essential for mammalian g

111 a Caenorhabditis elegans homologue of human flap endonuclease-1 (FEN-1) that is normally involved in

112 Human flap endonuclease-1 (Fen-1) was examined for its ability

113 Flap endonuclease-1 (FEN-1), a 43-kDa protein, is a stru

114 rimidinic endonuclease, DNA polymerase beta, flap endonuclease-1 (FEN-1), and PARP-1.

115 olog-1 (RTH-1) class nuclease, also known as flap endonuclease-1 (FEN-1).

116 Flap endonuclease-1 (FEN1) belongs to the Rad2 family of

117 Flap endonuclease-1 (FEN1) is a multifunctional, structu

118 Flap endonuclease-1 (FEN1) is proposed to participate in

119 The prototypical 5'-nuclease, flap endonuclease-1 (FEN1), catalyzes the essential remo

120 cture- and strand-specific phosphodiesterase flap endonuclease-1 (FEN1), the prototypical 5'-nuclease

121 Interaction between human flap endonuclease-1 (hFEN-1) and proliferating cell nucl

122 Human flap endonuclease-1 (hFEN-1) is highly homologous to hum

123 Human flap endonuclease-1 (hFEN1) catalyzes the essential remo

124 ion of Okazaki fragments escapes cleavage by flap endonuclease-1 and anneals to a complementary ectop

125 sion repair proteins DNA polymerase beta and flap endonuclease-1 by 4-6-fold.

126 pt annealing at the ectopic site and promote flap endonuclease-1 cleavage.

127 substrate recognition and specificity of the flap endonuclease-1 enzymes.

128 Bacteriophage T4 RNase H, a flap endonuclease-1 family nuclease, removes RNA primers

129 This is the first structure of a flap endonuclease-1 family protein with its complete bra

130 cates a back-up function of exonuclease 1 to flap endonuclease-1 in RNA primer removal during lagging

131 Flap endonuclease-1 or FEN-1 is a structure-specific and

132 The role of human FEN1 (flap endonuclease-1), an RTH1 (RAD two homolog-1) class

133 Addition of Flap endonuclease-1, a nuclease known to remove 5' overh

134 Finally, a combination of HIV-1 RT, Flap endonuclease-1, and DNA ligase is capable of quanti

135 ivity of HEX1-N2 is similar to that of human flap endonuclease-1, both in terms of turnover efficienc

136 lymerase-1 (PARP-1) and the BER participants flap endonuclease-1, DNA polymerase beta, and apurinic/a

137 es lacking genes encoding the orthologues of flap endonuclease-1, PCNA, and MutS.

138 or recombination that may overlap with human flap endonuclease-1.

139 action of the WRN gene product with human 5' flap endonuclease/5'-3' exonuclease (FEN-1), a DNA struc

140 on of the WRN gene product with the human 5' flap endonuclease/5'-3' exonuclease (FEN-1), a DNA struc

141 raction of the BLM protein with the human 5'-flap endonuclease/5'-3' exonuclease (FEN-1), a genome st

142 e 1 (hExo1) possesses both 5'exonuclease and flap endonuclease activities and plays a role in DNA rep

143 ell understood, its 5' to 3'-exonuclease and flap endonuclease activities may cleave intermediates th

144 rocessive 5'-3' exonuclease and secondary 5'-flap endonuclease activities participate in various DNA

145 Both the 5' to 3' exonuclease and flap endonuclease activities require a divalent metal co

146 It has both 5' nuclease and flap endonuclease activities.

147 hPCNA-mediated stimulation of both exo- and flap endonuclease activities.

148 activity but significant single- and double-flap endonuclease activities.

149 Mutation of the Arg-70 significantly reduced flap endonuclease activity and eliminated exonuclease ac

150 ystal structures of related enzymes with the flap endonuclease activity and propose that there are tw

151 rad27-G240Dp displayed a significant double-flap endonuclease activity but was devoid of exonuclease

152 mutant Rad27 and FEN-1 enzymes with partial flap endonuclease activity but without nick-specific exo

153 The flap endonuclease activity is less active relative to it

154 Furthermore, although the flap endonuclease activity of FEN1 E359K was unaffected,

155 ctions between Fen1 and hChlR1 stimulate the flap endonuclease activity of Fen1.

156 The flap endonuclease activity of HEX1-N2 is similar to that

157 Flap endonuclease activity of the wild type and mutant e

158 nant human CtIP and find that it exhibits 5' flap endonuclease activity on branched DNA structures, i

159 The native exonuclease also had strong flap endonuclease activity similar to that observed with

160 T4 RNase H also has a flap endonuclease activity that cuts preferentially on e

161 Flap endonuclease activity was demonstrated in oocyte nu

162 Escherichia coli, the Xenopus enzymes showed flap endonuclease activity, a unique feature of this cla

163 Each enzyme has a flap endonuclease activity, cutting at or near the junct

164 the bacterial transposase Tn10 contains a 3' flap endonuclease activity, suggesting a mechanistic par

165 lease activity and showed only a weak single-flap endonuclease activity.

166 s been no biochemical evidence demonstrating flap endonuclease activity.

167 l acidic residue results in complete loss of flap endonuclease activity.

168 uplex as the 5'-tail inhibits gene 6 protein flap endonuclease activity.

169 ' exonuclease activity of Rad2p and not its "flap endonuclease" activity and is absolutely dependent

170 esidue is conserved in the structures of all flap endonucleases analysed to date.

171 esindicates that GST-Rad2p possesses both 5'-flap endonuclease and 5'-->3' double-stranded DNA exo-nu

172 suggest that PCNA mediates the entry of the flap endonuclease and DNA ligase I into the process of O

173 rs are removed from Okazaki fragments by the flap endonuclease and DNA ligase I joins nascent fragmen

174 SAV6 is an FEN1 homologue that shows double-flap endonuclease and gap-dependent endonuclease activit

175 Next, DNA repair activities of DNA ligase, flap endonuclease and RNase H2 were monitored.

176 Contacts between the 5' flap endonuclease and the sugar-phosphate backbone of th

177 PCNA, or reverse transcriptase), a nuclease (flap endonuclease), and a ligase (ligase I, III, or IV a

178 eat tracts, mutations of RAD27, encoding the flap endonuclease, and CDC9, encoding DNA ligase I, incr

179 lycosylase, AP endonuclease, DNA polymerase, flap endonuclease, and DNA ligase activities.

180 The enzyme has 5'-3' exonuclease, flap endonuclease, and weak RNaseH activity in vitro, bu

181 and RAD1, encoding part of the Rad1/Rad10 3'-flap endonuclease, caused synthetic growth defects in ye

182 Deletion of RAD27, which encodes a flap endonuclease, causes inviability in mre11 strains.

183 The 5'-flap endonuclease Fen-1 is essential for Okazaki fragmen

184 EN) activities but retains almost all of its flap endonuclease (FEN) activity, we show severe defects

185 The flap endonuclease (FEN) of the hyperthermophilic archaeo

186 ndonuclease activity that is provided by the flap endonuclease (FEN-1) in the nucleus, resulting in m

187 Flap endonuclease (FEN-1) removes 5' overhanging flaps i

188 e repeats form structures that inhibit human flap endonuclease (FEN-1).

189 ermediates became substrates for cleavage by flap endonucleases (FEN-1 proteins).

190 In the presence of the flap endonuclease FEN1 (Rad27), the complex carried out

191 2 heterodimer, bound to a single copy of the flap endonuclease FEN1 at 2.9 A resolution.

192 The conserved, structure-specific flap endonuclease FEN1 cleaves 5' DNA flaps that arise d

193 nd ubiquitylation, cooperate to target human flap endonuclease FEN1 to degradation by the proteasome

194 flap pathway, Pol delta coordinates with the flap endonuclease FEN1 to degrade initiator RNA, whereas

195 ised by RNase H2, and further excised by the flap endonuclease FEN1 with strand displacement synthesi

196 dinated actions of DNA polymerase delta, the flap endonuclease FEN1, and DNA ligase I.

197 of DNA polymerase delta (Pol delta) and the FLAP endonuclease FEN1.

198 ynthetically lethal with mutations in the 5'-flap endonuclease FEN1/Rad27 in Saccharomyces cerevisiae

199 e that the exonuclease activity of mammalian flap endonuclease (FEN1) excises Pol alpha replication e

200 to cut at the base of single-stranded flaps, flap endonuclease (FEN1) is now recognized as a central

201 Flap endonuclease (Fen1) is required for DNA replication

202 idence indicates that null mutants of the 5'-flap endonuclease (FEN1) result in an expansion of repet

203 rate to a double flap that can bind Dna2 and flap endonuclease (FEN1) simultaneously.

204 combined activities of polymerase B (PolB), flap endonuclease (Fen1), and DNA ligase are required to

205 Flap endonuclease (FEN1), essential for DNA replication

206 Pol beta activity is enhanced by flap endonuclease (FEN1), which cleaves the resulting fl

207 ught to be removed by RNase HI and the 5'-3' flap endonuclease (FEN1).

208 The flap endonuclease, FEN1, is an evolutionarily conserved

209 The flap endonuclease, FEN1, plays a critical role in DNA re

210 Flap endonucleases (FENs) catalyse the exonucleolytic hy

211 During replication and repair flap endonucleases (FENs) catalyze endonucleolytic and e

212 Flap endonucleases (FENs) isolated from archaea are show

213 The flap endonucleases (FENs) participate in a wide range of

214 Flap endonucleases (FENs), essential for DNA replication

215 ed member of a novel subfamily of ubiquitous flap endonucleases (FENs), which possess only one of the

216 The Invader technology uses a Flap Endonuclease for allele discrimination and a univer

217 cloned three open reading frames encoding a flap endonuclease from Archaeglobus fulgidus, Methanococ

218 mensional model of the structure-specific 5' flap endonuclease from Pyrococcus furiosus in its comple

219 phosphate diester hydrolysis catalysed by a flap endonuclease has been studied.

220 Rad2, a FEN-1 flap endonuclease homolog, is involved in processing Oka

221 role of one of the DNA replication factors, flap endonuclease I (FEN1), in regulating telomerase act

222 a 1- or 10-nucleotide flap DNA substrate by Flap Endonuclease I.

223 show the importance of the C terminus of the flap endonuclease in DNA replication and repair and, by

224 Crenarchaeal XPF, a 3'-flap endonuclease, is also stimulated by PCNA in vitro.

225 We recovered the flap endonuclease mutation rad27-K325* with a stop codon

226 ol delta depended on RAD1, which encodes the flap endonuclease needed to cleave MMEJ intermediates be

227 The flap endonucleases, or 5' nucleases, are involved in DNA

228 removed by strand displacement synthesis and flap endonuclease processing via a long patch repair mec

229 In addition, S. pombe mutant for the flap endonuclease rad2 gene, whose precise function in D

230 haromyces cerevisiae, the MMR system and the flap endonuclease Rad27 act in overlapping pathways that

231 teraction of DNA polymerase delta and the 5'-flap endonuclease Rad27/Fen1 with the PCNA sliding clamp

232 Mutants lacking the flap endonuclease Rad27p showed little change in the exp

233 Flap endonucleases remove flap structures generated duri

234 Genetic analysis revealed that the flap endonucleases Slx4 and Sae2 represent new pathways

235 ase 1 (Exo1) is a 5'-->3' exonuclease and 5'-flap endonuclease that plays a critical role in multiple

236 A replication and repair, is the major human flap endonuclease that recognizes and cleaves flap DNA s

237 To further refine the model, 5' flap endonuclease variants with alanine point substituti

238 Dna2, a flap endonuclease with 5'-3' helicase activity, is invol

239 tion-coupled ICL repair, we show that the 3' flap endonuclease XPF-ERCC1 cooperates with SLX4/FANCP t