ライフサイエンスコーパス: flavocytochrome b

1 heterodimer known as flavocytochrome b(558) (flavocytochrome b).

2 heme binding and biosynthetic maturation of flavocytochrome b.

3 phagosomes despite the continued presence of flavocytochrome b.

4 ane, where they assemble with membrane-bound flavocytochrome b.

5 in the activation process is transferred to flavocytochrome b.

6 The disordered loop region found in flavocytochrome b(2) and its close homologues remain unr

7 mutant protein and, by extension, wild-type flavocytochrome b(2) and the other flavoproteins catalyz

8 Flavocytochrome b(2) catalyzes the oxidation of L-lactat

9 Yeast flavocytochrome b(2) catalyzes the oxidation of lactate

10 Flavocytochrome b(2) catalyzes the oxidation of lactate

11 By comparison with the equivalent residue in flavocytochrome b(2) from Saccharomyces cerevisiae, argi

12 pendently expressed flavin-binding domain of flavocytochrome b(2) to 2.50 A resolution and compared t

13 wo homologous enzymes, glycolate oxidase and flavocytochrome b(2), indicated that a conserved arginin

14 ired the simultaneous presence of p47(phox), flavocytochrome b-245, and the anionic amphiphile.

15 es of recombinant Phox proteins and purified flavocytochrome b-245.

16 (O(2)) by reducing molecular oxygen through flavocytochrome b(558) (flavocytochrome b), a heterodime

17 through a transmembrane heterodimer known as flavocytochrome b(558) (flavocytochrome b).

18 obial killing, and includes a membrane-bound flavocytochrome b(558) and cytosolic p67(phox), p47(phox

19 sequential interactions between Rac and the flavocytochrome b(558) and p67(phox) oxidase components

20 f the respiratory burst and up-regulation of flavocytochrome b(558) are dependent on p38 MAPK but not

21 ly of cytosolic p47(phox) and p67(phox) with flavocytochrome b(558) at the membrane is crucial for ac

22 ene for gp91(phox), the large subunit of the flavocytochrome b(558) heterodimer, account for the majo

23 1 and Rac2, was able to specifically bind to flavocytochrome b(558) in vitro.

24 NL7 recognized the gp91(phox) flavocytochrome b(558) subunit by immunoblot and bound t

25 closely related GTPase Cdc42 at the level of flavocytochrome b(558) that regulates the formation of R

26 vity is by increasing membrane expression of flavocytochrome b(558) through exocytosis of intracellul

27 bearing polypeptide core of human neutrophil flavocytochrome b(558) was isolated by applying high per

28 reviously to increase membrane expression of flavocytochrome b(558), a component of the NADPH oxidase

29 x), a subunit of the NADPH oxidase component flavocytochrome b(558), also is phosphorylated.

30 mAb NL7 was raised against purified flavocytochrome b(558), important in host defense and in

31 unit of the membrane-bound oxidase component flavocytochrome b(558), is an in vitro substrate for bot

32 (phox), which together with gp91(phox) forms flavocytochrome b(558), the catalytic core of NADPH oxid

33 Flavocytochrome b(558), the catalytic core of the phagoc

34 the membrane component of the NADPH oxidase, flavocytochrome b(558), was actively excluded or rapidly

35 vesicles and specific granules that contain flavocytochrome b(558), with similar time courses and co

36 This fragment was recruited in a flavocytochrome b(558)-dependent, p67(phox)-specific, an

37 Falpha also increases membrane expression of flavocytochrome b(558).

38 p91(phox), the larger subunit of the oxidase flavocytochrome b(558).

39 cular oxygen through flavocytochrome b(558) (flavocytochrome b), a heterodimeric oxidoreductase compo

40 Surface expression of flavocytochrome b and heterodimer formation were relativ

41 omal colocalization of flavocytochrome b558 (flavocytochrome b) and p47phox and p67phox (p47/67phox).

42 22(phox), which form the membrane-integrated flavocytochrome b, and cytosolic subunits p47(phox) and

43 represent a site of binding interaction for flavocytochrome b, and this observation was confirmed in

44 marily hydrophobic NH(2)-terminal regions of flavocytochrome b are responsible for heme ligation.

45 ing assays, we also found that p67(phox) and flavocytochrome b competed for binding to p47(phox)after

46 The integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the h

47 The heterodimeric, integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the p

48 al features of the integral membrane protein flavocytochrome b (Cyt b) were discovered using an antib

49 s a heterodimeric integral membrane protein (flavocytochrome b (Cyt b)) that generates superoxide and

50 vity correlates with loss of p47/67phox from flavocytochrome b-enriched phagosomes and additional pho

51 Although in neutrophils flavocytochrome b has been shown to localize to the plas

52 roperties suggested that the majority of the flavocytochrome b heme environment remained intact.

53 These mutations also had no effect on the flavocytochrome b heme spectrum, although NADPH oxidase

54 Flavocytochrome b is a membrane heterodimer consisting o

55 murine bone marrow-derived macrophages that flavocytochrome b is found in the Rab11-positive recycli

56 These data support a model in which flavocytochrome b is required for stable membrane bindin

57 These studies show for the first time that flavocytochrome b localizes to intracellular compartment

58 Although each flavocytochrome b molecule contains two heme groups, the

59 phox) and p22(phox) to elucidate features of flavocytochrome b processing in myeloid cells.

60 ked detectable O(2)-generating activity, and flavocytochrome b purified from these cells contained li

61 We investigated the biosynthesis of flavocytochrome b subunits gp91(phox) and p22(phox) to e

62 partially purified Triton X-100-solubilized flavocytochrome b that had been exposed to endoproteinas

63 te to the plasma membrane and associate with flavocytochrome b to form the active superoxide-generati

64 ane, which may act as a reservoir to deliver flavocytochrome b to the cell surface and phagosome memb

65 our data demonstrate that both p67(phox) and flavocytochrome b utilize a common binding site in p47(p

66 o gp91(phox), heterodimers did not form, and flavocytochrome b was not expressed on the surface of ce

67 ve Pak bound directly to p22phox, suggesting flavocytochrome b was the oxidase-associated membrane ta

68 iochemical and functional features of normal flavocytochrome b with those in cells expressing gp91(ph