ライフサイエンスコーパス: floor plate

1 ns with precise trajectories parallel to the floor plate.

2 misguidance of commissural axons towards the floor plate.

3 EphB receptors are expressed at the ventral floor plate.

4 s in a Shh dosage-dependent reduction of the floor plate.

5 sonic hedgehog (Shh) from the notochord and floor plate.

6 he floor plate or extend directly across the floor plate.

7 ith, and regulated by, Shh expression in the floor plate.

8 and E3 epithelia originate from the anterior floor plate.

9 iminates primary motoneurons, but not medial floor plate.

10 ormally attracts motor neurons closer to the floor plate.

11 trajectory on the contralateral side of the floor plate.

12 ntributes to notochord, prechordal plate and floor plate.

13 d in the longitudinal plane, parallel to the floor plate.

14 hog (Shh), secreted by the notochord and the floor plate.

15 for commissural axons in the vicinity of the floor plate.

16 y1 expression is absent from the prospective floor plate.

17 one, most prominently in the alar region and floor plate.

18 of inductive signals from the notochord and floor plate.

19 notochord and have a reduced, discontinuous floor plate.

20 mmissural growth cones upon contact with the floor plate.

21 ment of ventral neuroectoderm, including the floor plate.

22 atic clusters, the motor nuclei, next to the floor plate.

23 Hedgehog, secreted by the notochord and the floor plate.

24 ll as agenesis of DA neurons in the midbrain floor plate.

25 o3.2 transcripts in axons that encounter the floor plate.

26 row along the opposite direction towards the floor plate.

27 ibuting to the axon guidance function of the floor plate.

28 midbrain dopaminergic (mDA) neurons and the floor plate.

29 ion in repelling neuron cell bodies from the floor plate.

30 s using guidance molecules released from the floor plate.

31 xial mesoderm that lies directly beneath the floor plate.

32 ntrolateral funiculus, and never reenter the floor plate.

33 of the chick embryo to the notochord and the floor plate.

34 le navigating their intermediate target, the floor plate.

35 g and turn anteriorly only after exiting the floor plate.

36 fically inactivated Shh in the notochord and floor plate.

37 of the neural tube in cells that make up the floor plate.

38 cretion, is conditionally knocked out in the floor plate.

39 nt5a, and Wnt5b) expressed in and around the floor plate.

40 itors for mDNs reside at the ventral midline floor plate, a region that also serves as a source of in

41 alon-diencephalon border and adjacent to the floor plate, a source of the secreted signaling molecule

42 mmissural neurons grow toward and across the floor plate, a specialized structure located at the vent

43 line of the neural tube, particularly in the floor plate, a structure with key functions in patternin

44 bryonic CNS, mDA originate from the midbrain floor plate, a ventral midline structure that is operati

45 The repulsion is mimicked by a soluble floor plate activity.

46 rious fates in adjacent tissues, such as the floor plate, after the completion of gastrulation.

47 commissural (ILC) axons travel away from the floor plate along an arcuate trajectory into intermediat

48 The floor plate, an essential ventral midline organizing cen

49 We report that Vema is expressed in the floor plate, an intermediate target for pathfinding comm

50 othened is required for induction of lateral floor plate and a subpopulation of hypothalamic cells an

51 e zebrafish Xnot homologue, display enhanced floor plate and adaxial muscle phenotypes.

52 Netrins are secreted by the floor plate and attract commissural axons toward the mid

53 ve rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the question of

54 10 was highest in the dorsal spinal cord and floor plate and exhibited complex patterning in several

55 t they extend axons that reach and cross the floor plate and express apparently normal levels of the

56 including notochord, prechordal mesendoderm, floor plate and forebrain.

57 We demonstrate here that floor plate and hypochord cells arise from distinct regi

58 Notch signaling promotes floor plate and hypochord fates over notochord, but has

59 ial identity of prechordal plate, notochord, floor plate and hypochord progenitors during gastrulatio

60 thalamic cells and for maintenance of medial floor plate and hypothalamic cells.

61 2 mouse mutants, which lack specifically the floor plate and immediately adjacent interneurons.

62 hevelled at the cilium base in the zebrafish floor plate and in mammalian renal cells.

63 Barx2 was also prominently expressed in the floor plate and in Rathke's pouch.

64 tions, in the presence of a mature notoplate/floor plate and in the absence of the notochord, the cha

65 ution of chick axial progenitor cells to the floor plate and inhibits contribution to the notochord.

66 gehog (Shh) is produced by the notochord and floor plate and is responsible for inducing ventral neur

67 The neurogenic potential of the floor plate and its underlying mechanisms remain unknown

68 f (formerly lefty-1) in the left side of the floor plate and Leftb (formerly lefty-2), nodal and Pitx

69 We propose that precursors of the floor plate and neural crest were present in a common an

70 es of Gli proteins have been performed using floor plate and neuronal induction assays in frog embryo

71 n the notochord lineage, suggesting that the floor plate and notochord arise from distinct precursors

72 that sonic hedgehog (Shh) secreted from the floor plate and notochord is required for specification

73 as detected in the mouse node from where the floor plate and notochord precursors derive.

74 ous work has indicated that signals from the floor plate and notochord promote chondrogenesis of the

75 phibians, can develop in the absence of both floor plate and notochord.

76 terns of Nr-CAM in the chick spinal cord and floor plate and on commissural axons, where Nr-CAM has b

77 from nearby organizing centers, such as the floor plate and paraxial mesoderm.

78 al axons grow long distances parallel to the floor plate and precisely maintain their positions using

79 ost-crossing axons allows expulsion from the floor plate and prevents recrossing.

80 dary motoneurons, but the presence of medial floor plate and primary motoneurons in smoothened mutant

81 os are cyclopean, show a significant loss of floor plate and primary motorneurons and display disrupt

82 evelopment, inferior olivary axons cross the floor plate and project from the caudal to the rostral h

83 t ntl function is required to repress medial floor plate and promote notochord fate in cells of the w

84 tube defects revealed the absence of lateral floor plate and secondary motoneurons, but the presence

85 -associated protein Vema is localized to the floor plate and the optic chiasm, intermediate targets l

86 at delimit motor axon trajectories, i.e. the floor plate and the rhombic lip.

87 ate boundaries, at the interface between the floor plate and the ventral funiculus.

88 e cyclops mutation leads to a loss of medial floor plate and to severe deficits in ventral forebrain

89 i3 requires endogenous Gli1 for induction of floor plate and V3 interneurons.

90 w that ephrin-B ligands are expressed in the floor plate and within a dorsal region of the embryonic

91 urce of Sonic Hedgehog (SHH) by ablating the floor plate and/or notochord, or inhibiting SHH function

92 erned in this axis as there is a roof plate, floor plate, and differing numbers and types of neurobla

93 jected their axons longitudinally within the floor plate, and failed to reach their normal exit point

94 h the transverse plane, perpendicular to the floor plate, and in the longitudinal plane, parallel to

95 issural axons before and as they crossed the floor plate, and Netrin-1 stimulation dramatically incre

96 easing anterior-to-posterior gradient in the floor plate, and that a directed source of Wnt4 protein

97 of interneurons that send their axons to the floor plate, and the NGN2 progenitors in the ventral dom

98 Sonic hedgehog (Shh) from the notochord and floor plate appears to generate a ventral-to-dorsal grad

99 hat are absent in Shh mutants, including the floor plate, are present in Shh opb double mutants.

100 gehog (Shh) secreted by the notochord and/or floor plate as a primary regulator of auditory cell fate

101 ges 13-14), will independently mature into a floor plate as assayed three criteria: (1) continued exp

102 Neuregulin 1 (NRG)-1, localized in the floor plate as well as in motor and sensory neurons, is

103 fate specification of the notochord and the floor plate, as well as signaling within and between the

104 n bilateral stripes just dorsolateral to the floor plate at E12.5.

105 Commissural neurons project axons across the floor plate at the spinal cord ventral midline.

106 pioneer a circumferential trajectory to the floor plate at the ventral midline directed by secreted

107 The establishment of the floor plate at the ventral midline of the CNS is depende

108 Induction of the floor plate at the ventral midline of the neural tube is

109 Here we present a novel floor-plate-based strategy for the derivation of human D

110 opriate attraction to Netrin produced by the floor plate because of an accumulation of DCC receptor f

111 s notochord within a population of notochord/floor plate bipotential cells through negative transcrip

112 s ephrin protein is expressed at the lateral floor plate boundaries, at the interface between the flo

113 matically affect mouse ventral forebrain and floor plate but produce minor defects in zebrafish.

114 ract pre-crossing axons toward the hindbrain floor plate, but is active in post-crossing guidance.

115 spinal cord are guided ventrally toward the floor plate, but subsequently cross the midline and sele

116 actions also resulted in an avoidance of the floor plate, but without inducing growth-cone collapse.

117 showed that disruption of the notochord and floor plate by diphtheria toxin (DTA)-mediated cell abla

118 and Notch signaling in specification of the floor plate, by late inductive or early allocation mecha

119 This result demonstrates that while the floor plate can serve as a source of long-range cues for

120 These results show that the notoplate/floor plate capacity to induce the medially directed mot

121 nduced transcription factor FoxA2 to specify floor plate cells and dorsally in combination with BMP s

122 heir receptors are reciprocally expressed on floor plate cells and longitudinally projecting axons in

123 uroepithelium in positions characteristic of floor plate cells and motor neurons.

124 ck spinal cord netrin-1 mRNA is expressed by floor plate cells and netrin-2 mRNA by neural epithelial

125 os results in the ectopic differentiation of floor plate cells and ventral neurons within the neural

126 Floor plate cells are a population of epithelial cells t

127 Floor plate cells are induced by sonic hedgehog (SHH) se

128 t interactions between commissural axons and floor plate cells are required to modulate the localizat

129 Notochord and floor plate cells are sources of molecules that pattern

130 i2 and Gli3 repress the ectopic induction of floor plate cells by Gli1 in co-injection assays and inh

131 s the only Gli gene expressed in prospective floor plate cells of frog embryos, we have investigated

132 Foxj1 in the formation of long motile cilia, floor plate cells produce cilia that are longer than the

133 specification but only reduced the number of floor plate cells that developed compared to control emb

134 demonstrate that vangl2 functions largely in floor plate cells to regulate caudal neuronal migration.

135 eedback loop triggered by Shh that restricts floor plate cells to the midline.

136 SCF is expressed by midline floor plate cells, and its receptor Kit, a receptor tyro

137 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function.

138 rganized by Sonic hedgehog (Shh) secreted by floor plate cells.

139 only Gli1 can induce the differentiation of floor plate cells.

140 eural tube expressed FP-1, characteristic of floor plate cells.

141 es that correlated with remnants of apparent floor plate cells.

142 he basolateral, but not apical, membranes of floor plate cells.

143 to many notochord cells overlaid by a row of floor-plate cells.

144 In the embryonic spinal cord, the floor plate chemoattractant Netrin-1 is required to guid

145 Although hindbrain floor plate cilia are disorganized in vangl2 mutant embr

146 transient Shh signals from the notochord and floor plate confer a competence in somitic tissue for su

147 Thus, pioneer axons depend on long-range floor plate cues, with Slit/Robo signaling required for

148 neurons whose generation is specified by the floor plate-derived morphogenic signal sonic hedgehog (S

149 hed online at Nature-independently show that floor plate-derived netrin-1 is dispensable for commissu

150 Our results show that floor plate-derived neuropilin-2 is developmentally regu

151 We discovered that a novel source of floor plate-derived, but not axon-derived, neuropilin-2

152 Robo3.2 translation is induced by floor-plate-derived signals as axons cross the spinal co

153 d its midline derivatives, the notochord and floor plate, develop normally in both categories of muta

154 ated by the Ntl protein acting to antagonize floor plate development as well as to promote notochord

155 rmation and promoting hypochord and possibly floor plate development, but the precise mechanisms by w

156 ingly in ntl or spt enhance posterior medial floor plate development.

157 al mesoderm cells is not a pre-requisite for floor plate differentiation and suggest parallels betwee

158 1 and Shh may define the lateral boundary of floor plate differentiation.

159 n co-injection assays and inhibit endogenous floor plate differentiation.

160 lls and mediates the effects of Shh inducing floor plate differentiation.

161 Ectopic induction or genetic deletion of the floor plate diverted longitudinal axons into abnormal tr

162 The definition of the floor plate domain, therefore, appears to be defined by

163 ngly, mAb SAC1 also labels the notochord and floor plate during most stages of spinal cord developmen

164 Since Slits are expressed in the septum and floor plate during the period when these tissues cause c

165 wo organizers: sonic hedgehog (Shh) from the floor plate (DV) and Fgf8 from the isthmus (AP).

166 og (Shh) expression through binding to a Shh floor plate enhancer (SFPE2).

167 comparisons narrowed the activity of the Shh floor plate enhancer to an 88-bp sequence within intron

168 are each required for activation of the Shh floor plate enhancer, whereas the Tbx site was required

169 he early neural midline, the future anterior floor plate, exists as a separate population of floor pl

170 Semaphorins and Slits, which regulate their floor plate exit and restrict their post-crossing trajec

171 cues of the Slit family were altered in the floor plate experiments, suggesting their involvement in

172 prevented the growth cones from entering the floor-plate explants.

173 Foxa2 also regulates the expression of floor plate factors that control axon trajectories aroun

174 ted with differentiation of these cells to a floor plate fate reveals a role for the nascent prechord

175 h Shh to induce the epiblast precursors to a floor-plate fate.

176 ong period when their axons extend along the floor plate, following which they develop additional tro

177 than one inductive event is associated with floor plate formation along the length of the neuraxis.

178 To begin to reconcile models of floor plate formation in the vertebrate neural tube, we

179 n node and notochord, and can induce ectopic floor plate formation when misexpressed in the developin

180 re required non cell-autonomously for medial floor plate formation, suggesting that an inducing signa

181 ue development, likely prior to notochord or floor plate formation.

182 o a repulsive cue expressed at the embryonic floor plate (FP) and also the injured adult CNS.

183 Netrin1 has been proposed to act from the floor plate (FP) as a long-range diffusible chemoattract

184 e spinal cord, ephrin-B3 is localized to the floor plate (FP) at the ventral midline (VM), ephrin-B1

185 vels, Shh produced by both the notochord and floor plate (FP) diffuses dorsally to organize patterned

186 The floor plate (FP) is a midline signaling center, known to

187 gehog (SHH) source in the limb, the SHH-rich floor plate (FP) is reduced in the talpid2 midbrain.

188 the developing nervous system including the floor plate (FP) of the spinal cord where its function i

189 ow towards the midline in the absence of the floor plate (FP), a glial structure occupying the midlin

190 mmissural axons extend toward and across the floor plate (FP), an intermediate target at the ventral

191 gside or significant distances away from the floor plate (FP).

192 midline, send axons ventrally and across the floor plate (FP).

193 idline and functionally resemble the midline floor plate glia of the vertebrate spinal cord.

194 n in craniofacial cartilage, ear, notochord, floor plate, hypochord and fins in a pattern similar to

195 Nevertheless, floor plate identity and ciliogenesis are unaffected in

196 est parallels between the development of the floor plate in amniote and anamniote embryos.

197 pathfinding on the contralateral side of the floor plate in higher vertebrates.

198 While the role of the notochord and floor plate in patterning the dorsal-ventral (D/V) axis

199 t understanding the development of the early floor plate in the chick embryo.

200 pathfinding on the contralateral side of the floor plate in the embryonic rodent spinal cord.

201 is required for specification of the lateral floor plate in the frog.

202 ations in the ventral midline, including the floor plate in the spinal cord, the hindbrain and midbra

203 roper navigation of commissural axons to the floor plate in the spinal cord.

204 in guidance of commissural axons across the floor plate in the spinal cord.

205 e additional chemoattractant activity of the floor plate in vitro and can act directly as a chemoattr

206 e additional chemoattractant activity of the floor plate in vitro and for the normal projection of co

207 ormal projection of commissural axons to the floor plate in vivo.

208 ocalization within the basement membrane and floor plate in vivo.

209 We also find that the medial floor plate in Xenopus comprises two distinct population

210 The midline is similar to the vertebrate floor plate, in that it plays an essential role in cell

211 hat are generated in the region flanking the floor plate, including dopaminergic and serotonergic neu

212 nferior olivary axons; prior exposure to the floor plate increases responsiveness to these cues.

213 Floor-plate-inducing ability is retained when cytoplasmi

214 pts neurulation by permitting more extensive floor plate induction by Shh, thereby inhibiting midline

215 To understand molecularly how floor plate induction proceeds we identified a Shh-respo

216 required for axons to project away from the floor plate into the lateral funiculus.

217 For many axons, the floor plate is a source of long- and short-range guidanc

218 mpared to that of floating head mutants; the floor plate is almost complete and an anterior notochord

219 the notable exception that posterior medial floor plate is completely absent.

220 The floor plate is known to be a source of repellent signals

221 ord does not develop, and in cyc mutants the floor plate is nearly entirely missing.

222 t the mouse midbrain, but not the hindbrain, floor plate is neurogenic, giving rise to dopamine (DA)

223 Finally, we show that the floor plate is not required for the early trajectory of

224 edgehog (Shh), produced by the notochord and floor plate, is proposed to function as an inductive and

225 rise in close proximity to the notochord and floor plate, it has been assumed that their development,

226 r, our study shows that a subset of anterior floor plate-like cells gives rise to Fgf3(+) SOX3(+) pro

227 We show that the downregulation of Shh in floor plate-like cells in the forebrain governs their pr

228 The mesodiencephalic floor plate (mdFP) is the source of diverse neuron types

229 ural axons are observed crossing the ventral floor plate midline perpendicularly at about 20 microns/

230 ngly, our studies identified a rostral brain floor plate Neurog2 enhancer that requires direct input

231 ta-catenin signaling and Shh may orchestrate floor plate neurogenesis or a lack thereof.

232 evelopment of the vertebrate dorsal midline (floor plate, notochord, and hypochord) has been an area

233 floorplate, is not found in the prospective floor plate of nt embryos.

234 In cells of the neural tube floor plate of p190 RhoGAP mutant mice, polymerized acti

235 let1(+) motor neuron cell bodies invaded the floor plate of Robo1/2 double mutant mouse embryos or Sl

236 are thought to arise from progenitors in the floor plate of the caudal diencephalon and midbrain.

237 dance of commissural growth cones across the floor plate of the embryonic chicken spinal cord.

238 In addition, netrin-1 mRNA is found in the floor plate of the embryonic nervous system, an intermed

239 ce cue expressed in the ventricular zone and floor plate of the embryonic neural tube.

240 2-regulated enhancers that are active in the floor plate of the midbrain or along the length of the e

241 h it is expressed in an adjacent tissue: the floor plate of the nerve cord.

242 FOXA2, which marks the floor plate of the neural tube and definitive endoderm,

243 t for patterning of the ectodermally derived floor plate of the neural tube and the mesodermally deri

244 The notochord and the floor plate of the neural tube provide a ventralizing si

245 e notochord, dorsal foregut, and part of the floor plate of the neural tube.

246 and was coexpressed with Shh in the ventral floor plate of the neural tube.

247 alisation with that of Sonic hedgehog in the floor plate of the neural tube.

248 n's node such as endoderm, notochord and the floor plate of the neural tube.

249 from one of their intermediate targets, the floor plate of the spinal cord.

250 ute two orthogonal turns before crossing the floor plate or extend directly across the floor plate.

251 s on the presence of either a differentiated floor plate or notochord.

252 itor cells that can contribute to either the floor plate or the notochord.

253 or plate, exists as a separate population of floor plate precursor cells in the epiblast of the gastr

254 Midbrain floor-plate precursors are derived from PSCs 11 days aft

255 The floor plate produces several types of guidance cues with

256 anding large numbers of homogeneous midbrain floor plate progenitors (mFPPs) that retain efficient DA

257 aled that Notch signaling maintains dividing floor plate progenitors.

258 l commissural (MLC) axons grow alongside the floor plate, projecting primarily in the rostral directi

259 , including stalling and knotting inside the floor plate, recrossing, randomized anterior-posterior p

260 ) specifies a ventral progenitor fate in the floor plate region that later gives rise to mDA neurons.

261 e for conferring proper cell identity in the floor-plate region of midbrain and does not require an O

262 n post-crossing commissural axons to mediate floor plate repulsion in the mouse spinal cord.

263 motor axons when co-cultured with septum or floor plate respectively.

264 placement of the notochord from the midbrain floor plate resulted in abnormal folding and overall col

265 In the mouse cochlea and in the zebrafish floor plate, Rpgrip1l was required for positioning the b

266 , prechordal plate, notochord, hypochord and floor plate share a common embryonic origin.

267 ional transcription factors expressed in the floor plate share overlapping functions with Foxj1.

268 the ventral midline of the neural tube, the floor plate, share the property of being a source of the

269 t is under positive control by notochord and floor plate (Shh), dorsal neural tube (Wnt1) and surface

270 ultures, olivary axons which had crossed the floor plate showed an increased tendency to respond to c

271 transiently at the contralateral edge of the floor plate, showing a delay in midline exit.

272 ts support the view that either notochord or floor plate signaling can specify Lim3-positive motoneur

273 Floor plate-specific deletion of neuropilin-2 significan

274 F-spondin; (2) the display of the notoplate/floor plate-specific randomly oriented protrusive activi

275 otochord decisions and plays a minor role in floor plate specification, where it acts in parallel to

276 ally in the mouse Gli2 mutant that lacks the floor plate, suggesting an evolutionarily conserved role

277 he expression of all three Slit genes in the floor plate suggests that they are likely to share the s

278 gical and functional features of the amniote floor plate that distinguish these cells from the rest o

279 experiments, we showed that in the zebrafish floor plate the function of Rpgrip1l in planar polarity

280 lf extends many cell diameters dorsal to the floor plate, the site of netrin-1 expression.

281 though Slits are expressed in the septum and floor plate, their proteins do not contribute to the maj

282 e hindbrain roof plate; and that, unlike the floor plate, these dorsal organizing centers develop in

283 We have found that the floor plate throughout the midbrain, hindbrain and spina

284 ance mechanisms in vivo, the overall role of floor plate tissue and the specific roles of Slit/Robo s

285 Here, we show that notochord or floor plate tissue can induce the formation of ectopic s

286 ord, sonic hedgehog (Shh) is secreted by the floor plate to control the generation of distinct classe

287 Commissural filopodia appear on the floor plate to interact with the nascent neural network,

288 though commissural axons readily entered the floor plate under control conditions, perturbations of e

289 After initial attraction to the floor plate using Netrin1 activation of its main attract

290 Likewise, signals from notochord and floor plate ventralise the somite, at high levels overri

291 find that Shh signaling is not required for floor plate vs. notochord decisions and plays a minor ro

292 f hypochord at the expense of notochord, but floor plate was not affected.

293 st establish that the notoplate (presumptive floor plate), when separated from the underlying notocho

294 loping neural tube, but is excluded from the floor plate where Sonic hedgehog (Shh) is expressed.

295 spinal cord send axons ventrally toward the floor plate, where they cross the midline and turn anter

296 f Shh decreased locally in the notochord and floor plate, whereas overt patterning and differentiatio

297 we cocultured cortical explants with E13 rat floor plate, which expresses Netrin-1, or with Netrin-1-

298 be Cdo is expressed within the Shh-dependent floor plate while Boc expression lies within the dorsal

299 y 1/2 and activation of SMADs 2 and 3 at the floor plate, while cell fate specification of the notoch

300 n and spinal cord despite the absence of the floor plate, while no tail mutant embryos, which lack a