ライフサイエンスコーパス: flow cell

1 tested before being reincorporated into the flow cell.

2 n electrode (SPE) that was integrated into a flow cell.

3 ted using continuous methanol oxidation in a flow cell.

4 o binding of a protein to sequenced DNA in a flow cell.

5 mobilized at both ends within a microfluidic flow cell.

6 wn spectroscopy (CRDS) in a Brewster's angle flow cell.

7 eaction spots simultaneously within a single flow cell.

8 chromatograph and then introduced into a MCA flow cell.

9 tween two neoprene O-rings within the liquid flow cell.

10 ormation of precipitate in a 3D porous media flow cell.

11 l culture and the other serving as a reagent flow cell.

12 and a previously described Brewster's-angle flow cell.

13 dex of the solutions in the SPR microscope's flow cell.

14 introduction of the hydrodynamic-addressing flow cell.

15 in refractive index of the solutions in the flow cell.

16 custom-built 1.7-cm path length fiber-optic flow cell.

17 system as a controlled-current electrolytic flow cell.

18 imately 0.2 microM with a 1.5-mm-path length flow cell.

19 situ and then incorporated directly into the flow cell.

20 ) can be detected using this technology in a flow cell.

21 beginning of the analyte band within the NMR flow cell.

22 under flow of buffer through a microfluidic flow cell.

23 analysis points across a 2.3 mm wide optical flow cell.

24 imultaneous sensing of K and Na ions using a flow cell.

25 ture moving fluorescently labeled cells in a flow cell.

26 r durability and integrated into a two piece flow cell.

27 lyte to a capture spot within a microfluidic flow cell.

28 tu library construction on standard Illumina flow cells.

29 development on immersed surfaces inside the flow cells.

30 e used to quantify the biomass in saliva-fed flow cells.

31 ns was observed in three-species biofilms in flow cells.

32 953 were unable to grow as single species in flow cells.

33 hickness and 574-fold increase in biomass in flow cells.

34 erimentally the velocity fields inside these flow cells.

35 n both static culture and continuous-culture flow cells.

36 a PDMS micro-sized reaction chamber and two flow cells.

37 rapidly mixed with protein injected into two flow cells.

38 systems such as microtitre plate assays and flow cells.

39 ich dominates the cycling stability of these flow cells.

40 on are included and compared to conventional flow cells.

41 ously in flowing liquid samples using custom flow-cells.

42 ure drop produced at the scale of individual flowing cells.

43 s packed into FEP Teflon tubing to produce a flow cell (0.2-mL free column volume), which is placed i

44 wraparound design was used as a small-volume flow cell (5 microL) to introduce antibody solutions to

45 re performed using a 12-channel microfluidic flow cell after optimizing surface modification protocol

46 Use of a flow cell allowed diffraction patterns to be recorded, w

47 iguration in combination with a microfluidic flow cell, allowed samples of 5 muL to be sequentially m

48 All the stages are performed in a single flow cell allowing application of electric and magnetic

49 trodes (RAM) integrated into a purpose-built flow cell, allowing combined diffusion and convection to

50 The SLBs were patterned inside a flow cell, allowing for various concentrations of tetrac

51 to alter the flow rates in the microfluidic flow cells allows a variety of time scales to be studied

52 The use of an electrochemical flow cell also enables control of the timing and extent

53 Previous flow cell analyses demonstrated that QS and PZs are invo

54 m UAMS-1 harvested from a biofilm grown in a flow cell and compared the transcriptional profile of th

55 A quartz flow cell and experimental optimization of the signal-to

56 silica surface in a radial stagnation point flow cell and in a micromodel, which has 2-dimensional (

57 mplete sensor system, including microfluidic flow cell and surface functionalization with glycan rece

58 ich depend on the particular geometry of the flow cell and the flow rate used.

59 icles (DENs) have been incorporated into the flow cell and their electrocatalytic properties evaluate

60 by static microtiter and continuous culture flow cell and tubing biofilm assays.

61 We used an in-line flow cell and water suppression experiments to determine

62 s fibrous proteins can be aligned in Couette flow cells and characterized using LD.

63 ew USA200 and USA300 clonal types in biofilm flow cells and measured the extraction and accumulation

64 Preparation of flow cells and reagents requires 2 days each; in situ DN

65 fabricate both single- and multiple-channel flow cells and the advantages of each design for differe

66 Capture and isolation of flowing cells and particulates from body fluids has enor

67 0 nmol; MW = 497 g/mol) was delivered to the flow cell, and a gradient correlation spectroscopy spect

68 PV), more were attached to the center of the flow cell, and particles were attached to both the upstr

69 42-fold coverage was obtained from a single flow cell, and the best pooled data assembly produced a

70 acterized the hydrodynamic properties of the flow cell, and the force acting on the proteoliposomes d

71 de coated slides mounted in a parallel-plate flow cell, and their inactivation rate (loss in membrane

72 nalysis were used to quantify the biomass in flow cells, and quantitative real-time PCR with species-

73 lyst solution through the resin, heated in a flow cell apparatus.

74 n-to-carbon ratio were predicted utilizing a flow cell apparatus.

75 Development of a flow-cell apparatus further exemplifies its suitability

76 Through the use of a flow cell, aqueous samples containing cyanide are reacte

77 ulations, optimized geometries of the sensor flow cell are proposed to further improve the response t

78 novel types of miniaturized electrochemical flow cells are described.

79 The flow cells are fabricated in fluorinated ethylene propyl

80 Microfluidic flow cells are used in single-molecule experiments, enab

81 With a sudden onset of flow, cells are exposed to both temporal and spatial gra

82 we report the integration of a microfluidic flow cell array (MFCA) with SPRM enabling in situ microa

83 lasmid partition system using a DNA-carpeted flow cell as an artificial nucleoid surface and magnetic

84 zed with enzyme, then it was integrated in a flow cell as an electrochemical biosensor.

85 Using flow cell assays, we found that these PZ-altered derivat

86 ed silica capillary and an in-house designed flow-cell assembly.

87 million template-containing microbeads in a flow cell at a density greater than 3x10(6) microbeads/c

88 binding calspermin to the surface of the CM5 flow cell at a sufficiently high concentration for SPR,

89 In flow cells, bacterial growth was quantified using fluore

90 Modern optics flow cells based on total internal reflection are includ

91 alf thymus DNA (ctDNA) were conducted with a flow-cell based quartz-crystal microbalance, and a bindi

92 kinetic capillary chromatography, and sheath flow cell-based LIF detection.

93 When reactions cannot be conducted in the flow cell because of temperatures or solvents not tolera

94 s ranging from 3 to 8 kb on the surface of a flow cell before in situ library construction, thereby e

95 Using a flow cell biofilm model, we showed that a sub-MIC of azi

96 ype strain produced large microcolonies in a flow cell biofilm model, while the abpA mutant strains g

97 is of confocal micrographs obtained from the flow cell biofilms revealed that phosphate limitation in

98 ons were initially analyzed in P. aeruginosa flow cell biofilms using liquid chromatography coupled w

99 ecovery could be replicated in plasma-coated flow cell biofilms.

100 This low-cost and easy to handle PAO-based flow-cell biosensor may serve as a basis for a future pl

101 esign for online continuous water monitoring flow-cell biosensor, incorporating recombinant reporter

102 aracteristics to the conventional LD Couette flow cell but with the added benefit of a much shorter d

103 However, flow cells can also lead to deterioration of the separat

104 Flow cells can lead to much improved performance.

105 DNA is acoustically sheared and loaded in a flow cell channel for single-molecule sequencing.

106 cells, were immobilized on the inside of the flow cell channel, thus forming a stationary phase of pr

107 The flow cell channels consist of two parallel gold surfaces

108 relation was demonstrated across independent flow-cell channels, instrument runs and sample preparati

109 ne electronic structure, we have developed a flow cell closed by a graphene membrane that provides el

110 e included the power-time dependencies under flow cell conditions, the sensors performance (evaluated

111 nm and demonstrate outstanding stability in flow cell conditions.

112 e grown as broth culture in a Taylor-Couette flow cell configured to provide continuous shear forces

113 mmetry have been evaluated in a microfluidic flow cell containing a 25 mum gold microband electrode.

114 O is detected amperometrically at a parallel flow cell containing a glassy carbon working electrode m

115 A thin-layer electrochemical flow cell coupled to capillary electrophoresis with cont

116 Continuous-flow cells coupled with confocal microscopy corroborated

117 Thus, the flow cell described herein is a suitable tool for shotgu

118 l attention to capillary connections and NMR flow cell design allows on-line NMR detection with no si

119 A state-of-the-art flow cell design allows variable cell volumes as low as

120 However, flow cell design and microarray fabrication have hindere

121 d this has led to a number of innovations in flow cell design that make possible selective syntheses

122 The microfluidic flow cell design utilizes three-dimensional sheath flow

123 eometries consisted of a coil and a fountain flow cell design; the latter is based on radial solution

124 Shear flow cell detachment assays confirmed that lymphocytes c

125 ing, and scintillating anion exchange with a flow-cell detection system for a sequential measurement

126 Flow cell detectors were developed for simultaneous conc

127 A microreactor coupled to an electrochemical flow cell detects the biuret complex of the natively ele

128 A flow cell device, integrating different chip surfaces, w

129 The performance of a 48-flow-cell device was demonstrated through a series of st

130 treptococcus gordonii was investigated using flow cell devices that allow abiotic biofilm formation u

131 k mode imaging with a "wide" high throughput flow cell (e.g. flow rates set to 10 mL/min) in contrast

132 oscopy and by coupling of an electrochemical flow cell (EFC) with online inductively coupled plasma m

133 bolite of oral bacteria, was measured in the flow cell effluent of one-, two- and three-species commu

134 for TPrA(*+) in this medium was obtained via flow cell electron spin resonance (ESR) experiments at a

135 Our design of the flow cell enables the rapid movement of trapped molecule

136 Laser excitation of sample in a continuous flow cell enables time-resolved measurement of the initi

137 laser light source, a temperature-regulated flow cell encasing the sensing chip, motorized optics, a

138 he synthesized resin for use in a variety of flow-cell experiments utilizing a standard (129)I soluti

139 Interestingly, in flow cells F. nucleatum and A. actinomycetemcomitans exh

140 commensal isolate strain 129Pt were grown in flow cells, followed by analysis by confocal laser scann

141 " which can be less aggressive than wall-jet flow cell for a biological recognition element immobiliz

142 nd about 5 million polonies are arrayed in a flow cell for parallel sequencing.

143 d from the column to the Cherenkov detection flow cell for quantification and calculation of the orig

144 temperature-controlled renewable microcolumn flow cell for solid-phase nucleic acid hybridization in

145 ) the fluorescence detector and accompanying flow cell for the organic phase enriched with the reacti

146 A planar, chip-based flow cell for UV-vis absorbance detection in HPLC is pre

147 Using flow cells for biofilm formation, c-di-GMP showed a non-

148 on of time-varying composition into parallel flow cells for concurrent measurement of laser light sca

149 re solutions flow, being one of the simplest flow cells for FIA.

150 We demonstrate the potential of such flow cells for the development of high throughput, paral

151 Time-resolved SAXS in a flow cell format is a general method for connecting chem

152 face, and sICAM-1 binding was monitored in a flow cell format.

153 Using flow-cell FTIR measurements, we provide direct evidence

154 Evaluated flow cell geometries consisted of a coil and a fountain

155 igated the flow characteristics in different flow cell geometries relevant to the coupling of capilla

156 Depending on flow cell geometry and medium, the detection efficiencie

157 0% (CaF2(Eu)/Dowex 1 x 8-400(Cl) in fountain flow cell geometry).

158 The in-line flow cell gives excellent quality NMR spectra having lit

159 Examination of flow cell-grown biofilms showed that the sadB199 mutant

160 A novel microfluidic electrochemical channel flow cell has been constructed for in situ operation in

161 The flow cells, have a channel (duct) geometry, with cell he

162 to selenite immobilization in a microfluidic flow cell having a transverse mixing zone between propio

163 y and sequentially at 0.6 mL/min in a sample flow cell housing the sensor.

164 ction in a 50 cm liquid core waveguide (LCW) flow cell illuminated by a white light emitting diode.

165 The incorporation of the flow cell improves both analyte sensitivity and instrume

166 n) of the number of molecules present in the flow cell in the absence of chemical interferences.

167 enables internal referencing for individual flow cells in a parallel processing microfluidic network

168 We flow cells in suspension through a microfluidic channel

169 fecting flow cell operation and describe the flow cells in use at present for studying the interactio

170 zation MS with a traditional electrochemical flow cell, in which the electrolyzed solution emanating

171 The flow cell included a stepper motor-driven rotating rod w

172 sampling" chip, consisting of a microfluidic flow cell integrated onto the hybrid sensor, which demon

173 the capability of transforming a macroscale flow cell into a nanofluidic device without the need for

174 e results demonstrate that the concentration flow cell is a promising approach for efficiently harves

175 king electrode of an on-line electrochemical flow cell is accomplished via a nonelectrolytic adsorpti

176 A microstrip-line-based 0.3 muL flow cell is built and incorporated into the MIM.

177 The new flow cell is capable of analyzing 20 mL/min of fluoresce

178 equation for the optimal size of the sensor flow cell is developed to guide sensor design and valida

179 , we show that the design of our tip overlay flow cell is efficient at delivering sample to the subst

180 s method whereby the surface of a sequencing flow cell is modified in situ to capture specific genomi

181 physical relationship to one another on the flow cell is related to genomic distance.

182 At the heart of the flow-cell is a disposable chip made of porous aluminum o

183 The one channel flow-cell is attached and directly changeable with elect

184 lates the laminar flow of analyte within the flow cell, its diffusion both perpendicular and parallel

185 om the capillary is injected directly into a flow-cell joined to the tip of the outer capillary via a

186 The originality of the proposed flow cell lies in the possibility to adjust the pH of th

187 reaction was detected in an electrochemical flow cell localized outside of the reactor using a plati

188 projections of cells that extended into the flow cell lumen.

189 he flow-through CNT membrane integrated in a flow cell makes it an appealing approach not only for el

190 Using the flow cell method, we found that the chromosomal mutation

191 Promisingly, we can probe flow cells multiple times with antibodies, suggesting th

192 ed sample plug is passed to a potentiometric flow cell of 13 muL volume.

193 When reactions are conducted in the flow cell of the QCM in the presence of solvent, measure

194 ng target bioparticles from a flow stream in flow cells of 47.5 microL, achieving a 20-fold increase

195 y construction and optical sequencing on the flow cells of currently available massively parallel seq

196 used to couple a thin-layer electrochemical flow cell on-line with an ICP-mass spectrometer to perfo

197 plutonium by the coupling an electrochemical flow cell on-line with an ICPMS system.

198 Neisseria gonorrhoeae forms a biofilm in flow cells on glass coverslips as well as on primary cer

199 fibrinogen activate NF-kappaB in response to flow, cells on collagen or laminin do not.

200 The concentration flow cell only used inexpensive materials and did not re

201 ercially available, thin-layer, amperometric flow cell operated at a potential of only +0.05 V versus

202 ynthetic organic chemist can often be met by flow cells operating with recycle of the reactant soluti

203 discuss the fundamental processes affecting flow cell operation and describe the flow cells in use a

204 e force acting on the proteoliposomes during flow cell operation was estimated to be in the range of

205 surface of the waveguide using either a PDMS flow cell or laser exposure, producing width sizes rangi

206 ystematically varying the wettability of the flow cell over a wide range of contact angles, we find t

207 In three-species flow cells, P. gingivalis grew with Veillonella sp. and

208 retention of nanoparticles in a microfluidic flow cell packed with glass beads was studied with the u

209 fluorescent nanoparticles in a microfluidic flow cell packed with glass beads.

210 s of high-resolution imaging in microfluidic flow cells patterned with vertical posts.

211 rials are contained in a transparent plastic flow cell placed between two photomultiplier tubes for r

212 The details of flow cell preparation, protein-reconstituted vesicle pre

213 eting the binding kinetics in a large-format flow cell presents a number of potential challenges, inc

214 atalase or if the reaction is performed in a flow-cell, probably because oxygen is reduced to hydroge

215 ormed in batch (dip-and-dry) and continuous (flow-cell) processes, and binding performances were comp

216 The filtering flow cell provided in-line separation of small molecules

217 the hydrodynamic focusing properties of the flow cell, providing a clearer picture of the confinemen

218 The inner surface of the 1.5mm ID, 12microl flow cell quartz window has been modified with the aptam

219 When exposed to flow, cells rapidly (<2 min) and transiently decreased c

220 f the glass substrate, which is mounted in a flow cell reaction chamber connected to a DNA synthesize

221 s procedure was experimentally verified in a flow cell reactor using a pair of carbon electrodes (10(

222 In a radial stagnation point flow cell (RSPF), the deposition rate of a flagellated s

223 inclusive of array captures and one Illumina flow cell run.

224 s (% EE) of chiral molecules in time using a flow cell sampling apparatus is reported.

225 In this novel thin layer flow-cell screen-printed electrodes, the working electro

226 To assist the experimentalist in flow cell selection, we review the construction techniqu

227 aqueous-phase composition, this microfluidic flow cell should find wide applicability in many areas o

228 prelabeling step and are compatible with the flow cell, sparing the need to release the cells from th

229 sis system with a liquid waveguide capillary flow cell-spectrophotometric detection device.

230 designed and implemented a Brewster's angle flow cell such that cavity ring-down spectroscopy can be

231 this concept to practice, we fabricated two flow cells such that the mean linear fluid velocity, U,

232 protein to >10(7) RNA targets generated on a flow cell surface by in situ transcription and intermole

233 A flow cell surface of 0.2mm(2) with 50 cells was imaged e

234 a flow cell with a volume of ~50microl and a flow cell surface of 170mm(2).

235 A coupled RAM-flow cell system is implemented and is shown to give rep

236 Electrochemical detection within a flow cell system is then used to sense redox-active prod

237 In addition, we designed a novel flow cell system that allows mucosal biofilm formation u

238 nce contrast microscopy, coupled with a dual-flow cell system, to determine the effect of KSL on oral

239 lm, which was subsequently incorporated in a flow cell system.

240 rated and measured by a previously developed flow-cell system utilizing scintillating anion-exchange

241 uent was used to quantify IO3(-) by the same flow-cell system.

242 The introduction of liquid microjet and flow cell technologies into XAS experiments has enabled

243 ent a continuous-flow microelectroextraction flow cell that allows for electric field enhanced extrac

244 CapMix approaches to develop a concentration flow cell that can overcome these limitations.

245 d by the development of a low volume Couette flow cell that efficiently aligns long molecules in solu

246 nd application of a new micro-volume Couette flow cell that significantly enhances the potential appl

247 e have developed multinode acoustic focusing flow cells that can position particles (as small as a re

248 seawater was passed through custom-designed flow cells that enabled direct observation of TEPs and p

249 In the new flow-cell the injection is done through an "in-line luer

250 asma mass spectrometer coupled to a scanning flow cell, the activity and stability of non-noble elect

251 When using a sheath flow cell, the pH at the detection zone can be adjusted

252 llary is an enclosed system that acts as the flow cell, the waveguide, and the solid support for immo

253 le streams immediately prior to the reaction flow cells, the instrument is capable of automatically g

254 e analysis is performed without the use of a flow cell, thereby eliminating previously detrimental me

255 ference surface to be placed within the same flow cell, thereby improving data quality and extending

256 Flowing cells through PEFs is a promising technology for

257 y graphically representing data generated by flow cell tiles.

258 uidic system used a novel microbead-trapping flow cell to capture antibody-coupled beads with subsequ

259 aman spectroscopy, using a self-designed gas flow cell to control the reductive/oxidative environment

260 iments were conducted with a two-dimensional flow cell to examine the effect of monorhamnolipid surfa

261 Using an optical trap and a two-channel flow cell to move single DNA molecules into buffer conta

262 rs, and exposing the residual particles in a flow cell to near-ultraviolet (lambda>300 nm) radiation,

263 impermeable quencher was passed through the flow cell to quench the fluorescence of the outer layer.

264 imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample co

265 work, we used laboratory-scale porous media flow cells to examine a potential remediation strategy e

266 gn and fabrication of a microfluidic device (flow cell) used in conjunction with dual optical trappin

267 reduction peak of TNT in an electrochemical flow cell using a carbon working electrode and a Ag/AgCl

268 With a hybrid anode, Li|TEMPO flow cells using this electrolyte deliver an energy effi

269 For flow cell version R9.4, the estimated turnaround time fr

270 concentration required for saturation of the flow cell was determined to be 500 mug/mL.

271 The flow cell was found to bind a maximum of 1 mug proteolip

272 An IR flow cell was modified to enable the substrate containin

273 A custom-built flow cell was used to deposit benzenethiol from the vapo

274 d electrodes (SPE) integrated in one channel flow-cell was developed.

275 of the SLIM spectrometer, configured with a flow cell, was evaluated and compared to that of a comme

276 interactions on a high-throughput sequencing flow cell, we comprehensively assess the effects of comb

277 local velocity fields and dispersion in the flow cell were generated from MRI, while high resolution

278 t by confocal laser scanning microscopy when flow cells were used to grow biofilms.

279 We have developed a microfluidic flow cell where stepwise enzymatic digestion is performe

280 particles were attached to the center of the flow cell, where the pore-scale velocity is relatively h

281 ctrode device is employed, in a hydrodynamic flow cell, where the upstream electrode is used to contr

282 of 0.057 Bq/L using a 5 mL volume Cherenkov flow cell, which is below the drinking water limit of 0.

283 immobilized zone through an attached 3D-PDMS flow cell with 20-microm-deep microchannels.

284 grated chip is only 1cm(2) (including a PDMS flow cell with a 50microm height microfluidic channel fa

285 riments were conducted using a two-electrode flow cell with a voltage window of +/-0.9 V.

286 lines were grown as a mixed population in a flow cell with a volume of ~50microl and a flow cell sur

287 ed in a cuvette with one laser pulse or in a flow cell with an average of 4.6 pulses per sample.

288 Using a high-sensitivity UV flow cell with an extended path length of 1.2 mm, concen

289 detection module consists of a microfluidic flow cell with an integrated Complementary Metal-Oxide-S

290 a flow-through mode, we designed a miniature flow cell with interchangeable quartz window carrying im

291 ine coupling of a thin-layer electrochemical flow cell with liquid sample desorption electrospray ion

292 ses, we have developed an open, microfluidic flow cell with which surface physical properties can be

293 amics was used to simulate fluid flow inside flow cells with a volume of approximately 1 microL.

294 Microstereolithography was used to fabricate flow cells with defined hydrodynamics which minimize dis

295 substratum coverage (19-fold) in continuous-flow cells with minimal medium (growth rate not altered

296 id-phase polystyrene pegs (peg biofilms) and flow cells with saliva-coated glass surfaces.

297 Flow cells with saliva-coated surfaces are particularly

298 rved until 24 h) was profoundly inhibited in flow cells with seawater prefiltered to remove most larg

299 , through the use of a low-volume (4 microl) flow cell, with an ultrasensitive surface plasmon resona

300 The formation of enlarged flow cells within the active volume of the NMR detector