ライフサイエンスコーパス: fluorescence anisotropy

1 human serum albumin (HSA) was studied using fluorescence anisotropy.

2 ng to Fe-Fur and apo-Fur target sequences by fluorescence anisotropy.

3 ithin its matrix, this can result in a large fluorescence anisotropy.

4 aracterized its biophysical properties using fluorescence anisotropy.

5 protein, leading to a strong decrease in the fluorescence anisotropy.

6 mperature regulated high pressure cell using fluorescence anisotropy.

7 ing full-length HMGA1a protein binding using fluorescence anisotropy.

8 ect of nucleotides on this interaction using fluorescence anisotropy.

9 n fluorescence resonance energy transfer and fluorescence anisotropy.

10 d their binding to DNA was characterized via fluorescence anisotropy.

11 e SPR, isothermal titration calorimetry, and fluorescence anisotropy.

12 sed CTD motion or flexibility as measured by fluorescence anisotropy.

13 y tagging receptors and measuring changes in fluorescence anisotropy.

14 re detected and confirmed by competition and fluorescence anisotropy.

15 iation constant measured independently using fluorescence anisotropy.

16 conjugation was measured with time-resolved fluorescence anisotropy.

17 proteins, exhibits a seven-fold increase in fluorescence anisotropy.

18 ts and truncations of Ets-1 were analyzed by fluorescence anisotropy.

19 me resulted in a significant increase in the fluorescence anisotropy.

20 luorescently labeled DNA can be monitored by fluorescence anisotropy.

21 the D1D2 barrel of p97 that was confirmed by fluorescence anisotropy.

22 orescein tag to measure binding affinity via fluorescence anisotropy.

23 predict the glucose concentrations using the fluorescence anisotropy.

24 hemical analog of fluorescence polarization (fluorescence anisotropy), a versatile optical approach w

25 cently presented one such technique based on fluorescence anisotropy, a spectroscopic method based on

26 Using SEC HPLC and fluorescence anisotropy, absorption spectra were assigne

27 ow micromolar dissociation constants through fluorescence anisotropy after only two rounds of selecti

28 The fluorescence anisotropy, after an initial decay starting

29 -detection size-exclusion chromatography and fluorescence anisotropy allowed us to confirm that two d

30 Surface plasmon resonance and fluorescence anisotropy analyses demonstrated that hCpGA

31 Multisite FRET and fluorescence anisotropy analyses showed that S1 binding

32 tro using electrophoretic mobility shift and fluorescence anisotropy analyses.

33 The same effect is found for the fluorescence anisotropy analysis, for which the trans (c

34 s, electrophoretic mobility shift assay, and fluorescence anisotropy analysis, we determined key amin

35 s-isomer and was directly measurable through fluorescence anisotropy analysis.

36 d KaiA was stronger than normal, as shown by fluorescence anisotropy analysis.

37 omologous domains of p63 and p73 in vitro by fluorescence anisotropy, analytical ultracentrifugation

38 cells and are the active species as shown by fluorescence anisotropy and analytical ultracentrifugati

39 rget DNA sequence have been determined using fluorescence anisotropy and calorimetry.

40 opterin, incorporated into the P1 helix, and fluorescence anisotropy and catalytic activity were meas

41 tion of the RPEL(MAL):G-actin interaction by fluorescence anisotropy and cell reporter-based assays v

42 n binding site on cofilin, but we show using fluorescence anisotropy and chemical crosslinking that i

43 Using fluorescence anisotropy and electrophoretic mobility shi

44 Using fluorescence anisotropy and exploiting the symmetry of t

45 r such fluorescence changes were examined by fluorescence anisotropy and fluorescence intensity measu

46 Fluorescence anisotropy and fluorescence lifetime measur

47 We used fluorescence anisotropy and FRET imaging of Venus-tagged

48 onsensus RSS versus non-RSS substrates using fluorescence anisotropy and gel mobility shift assays.

49 Analysis of the sensitized fluorescence anisotropy and intensity decays indicates t

50 Fluorescence anisotropy and linear dichroism imaging hav

51 Results obtained from fluorescence anisotropy and NMR chemical shift mapping e

52 em conjugate via analysis of the time-domain fluorescence anisotropy and NMR chemical shift perturbat

53 The behavior of fluorescence anisotropy and polarization in systems with

54 d NS3, resulting in a hyperbolic increase in fluorescence anisotropy and providing an apparent equili

55 Fluorescence anisotropy and red edge excitation shift de

56 Fluorescence anisotropy and single molecule DNA stretchi

57 states were functionally characterized using fluorescence anisotropy and steady-state kinetics.

58 Fluorescence anisotropy and surface plasmon resonance ex

59 Fluorescence anisotropy and surface plasmon resonance in

60 Time-dependent fluorescence anisotropy and temperature-dependent Forste

61 Using both fluorescence anisotropy and time-resolved fluorescence q

62 oaches, which are surface plasmon resonance, fluorescence anisotropy, and capillary electrophoresis (

63 Combining surface plasmon resonance, fluorescence anisotropy, and circular dichroism (CD), we

64 nitored by intrinsic fluorescence intensity, fluorescence anisotropy, and circular dichroism and was

65 I)) and A(18) by a gel-mobility shift assay, fluorescence anisotropy, and fluorescence quenching.

66 tro using analytical ultracentrifugation and fluorescence anisotropy, and in living cells using two-p

67 including the fluorescence emission maximum, fluorescence anisotropy, and membrane bilayer penetratio

68 circular dichroism, fluorescence quenching, fluorescence anisotropy, and NMR.

69 Single-turnover kinetics, fluorescence anisotropy, and single-molecule fluorescenc

70 Here, using hydrogen/deuterium exchange, fluorescence anisotropy, and structural analyses, we sho

71 ng UV-vis absorption, fluorescence emission, fluorescence anisotropy, and two-photon absorption (2PA)

72 nsic Trp fluorescence, acrylamide quenching, fluorescence anisotropy, ANS binding, dynamic light scat

73 Using a fluorescence anisotropy approach, PEA-15 is shown to be

74 ry single-molecule fluorescence and ensemble fluorescence anisotropy approaches to discover how NNRTI

75 l mobilities that are inaccessible with bulk fluorescence anisotropy approaches, and anticipate that

76 To this end, we used fluorescence anisotropy as the transduction mechanism to

77 The dissociation constants obtained in the fluorescence anisotropy assay for binding of all compoun

78 entration dependence of ATP hydrolysis and a fluorescence anisotropy assay provided thermodynamic inf

79 A fluorescence anisotropy assay showed that Arp2 does not

80 Fluorescence anisotropy assay showed that FAM-UNO intera

81 Here, we have used a fluorescence anisotropy assay to demonstrate that eIF4G

82 del glycoprotein asialofetuin (ASF), using a fluorescence anisotropy assay to measure the concentrati

83 We used a novel fluorescence anisotropy assay to show that the specific

84 Using a competitive fluorescence anisotropy assay, we determined that monoph

85 Using a well established fluorescence anisotropy assay, we tested the direct inte

86 ia a virtual screen followed by testing in a fluorescence anisotropy assay.

87 bacterial two-hybrid assay and in vitro in a fluorescence anisotropy assay.

88 MSI family RRM domains using a quantitative fluorescence anisotropy assay.

89 cond-order acylation rate constants with the fluorescence anisotropy assay.

90 Finally, fluorescence anisotropy assays indicate that Fe-Fur spec

91 Fluorescence anisotropy assays on lambda Cro and the tri

92 this work, we used protein semisynthesis and fluorescence anisotropy assays to explore the interactio

93 ies using electrophoretic mobility shift and fluorescence anisotropy assays.

94 abasic DNA product using both band shift and fluorescence anisotropy assays.

95 CzrA formed complexes in gel-retardation and fluorescence-anisotropy assays with fragments of promote

96 is protein, we observed an unusual, negative fluorescence anisotropy at pH 6.0.

97 cal conformation, and a distinct increase in fluorescence anisotropy attributed to Tyr39 indicates an

98 isoform (p37(AUF1)) as a model, we employed fluorescence anisotropy-based approaches to define therm

99 that a previously reported high-throughput, fluorescence anisotropy-based assay for ATP-dependent re

100 We developed a fluorescence anisotropy-based assay for the binding of S

101 rrent article describes the development of a fluorescence anisotropy-based assay that mimics the prin

102 We previously described a fluorescence anisotropy-based assay to measure these rat

103 Using fluorescence anisotropy-based binding analysis and recom

104 We implemented a homogeneous fluorescence anisotropy-based binding assay in an automa

105 ations of electrophoretic mobility shift and fluorescence anisotropy-based binding assays, we show th

106 Fluorescence anisotropy-based DNA-binding analysis demon

107 Using a fluorescence anisotropy-based DNA-binding assay, we exam

108 roles of carboxylates were also observed in fluorescence anisotropy-based ligand-binding assays.

109 We report a simple, rapid, and reproducible fluorescence anisotropy-based method for measuring rate

110 Fitting these models to our fluorescence anisotropy binding data revealed that, surp

111 Fluorescence anisotropy binding measurements reveal that

112 s; combined with in vivo activity assays and fluorescence anisotropy binding measurements, these have

113 ese two mechanisms, we used a combination of fluorescence anisotropy, biolayer interferometry, and do

114 Here we show that measurements of fluorescence anisotropy can be used to determine the hyd

115 Consequently, ThT fluorescence anisotropy cannot be directly used to study

116 sed of 200 nM APTS-MT and 1 microM ConA, the fluorescence anisotropy capably tracks the concentration

117 Fluorescence anisotropy capillary electrophoresis (FACE)

118 The key factors involved in the fluorescence anisotropy change were considered through t

119 Using fluorescence anisotropy competition assays it is shown t

120 Fluorescence anisotropy competition binding experiments

121 Fluorescence anisotropy correlations, fluorescent lifeti

122 A good correlation between the fluorescence anisotropy data and separation data was obs

123 configurations, and experimentally collected fluorescence anisotropy data displayed the predicted tre

124 ulated from MD simulations with experimental fluorescence anisotropy data showed excellent agreement,

125 Absorbance, fluorescence, and fluorescence anisotropy data were collected concurrently

126 Our hydrodynamic studies using fluorescence anisotropy decay and analytical ultracentri

127 edge are novel assays based on time-resolved fluorescence anisotropy decay and dynamic quenching meas

128 Time-dependent fluorescence anisotropy decay measurements confirm that,

129 Fluorescence anisotropy decay measurements show that tig

130 Fluorescence anisotropy decay of fluorescently labeled N

131 ons where simulations accurately capture the fluorescence anisotropy decay, we find at most a modest,

132 ined from measurements of diphenylhexatriene fluorescence anisotropy decay.

133 Fluorescence anisotropy decays were employed to probe th

134 In this report, we demonstrate using a fluorescence anisotropy DNA-binding assay that the previ

135 tems where the fluorescence intensity and/or fluorescence anisotropy do not change upon interaction o

136 Changes were measured by fluorescence anisotropy, electron paramagnetic resonance

137 he certified values like absorption spectra, fluorescence anisotropy, excitation wavelength, and temp

138 Fluorescence anisotropy experiments at room temperature

139 Isothermal titration calorimetry and fluorescence anisotropy experiments corroborate these fi

140 gG Fc that is not conserved in IgA; however, fluorescence anisotropy experiments demonstrate that dir

141 Time-resolved fluorescence anisotropy experiments show that electronic

142 We utilized (i) time-resolved fluorescence anisotropy experiments to monitor the struc

143 absence of DNA in several assays, including fluorescence anisotropy experiments using a novel Alexa4

144 e modeled anisotropy decays to time-resolved fluorescence anisotropy experiments was obtained.

145 Addition of DNA to RAG1 and HMGB1 in fluorescence anisotropy experiments, however, results in

146 ences with similar affinities as measured by fluorescence anisotropy experiments.

147 Here, we have used fluorescence anisotropy (FA) and a panel of NCS-1 EF-han

148 Using competitive fluorescence anisotropy (FA) and electrophoretic gel mob

149 Herein, we design novel fluorescence anisotropy (FA) aptamer sensing platforms d

150 A direct and competitive fluorescence anisotropy (FA) assay to probe both the met

151 A fluorescence anisotropy (FA) competition-based Shc Src h

152 In standard steady-state fluorescence anisotropy (FA) DNA-based assays, the ligan

153 y optimized encoded sensors for quantitative fluorescence anisotropy (FA) measurements of protein hyd

154 R), tryptophan fluorescence quenching (TFQ), fluorescence anisotropy (FA), isothermal titration calor

155 splay a protein binding-induced reduction of fluorescence anisotropy (FA), which is exclusively diffe

156 scein, the K(d) = 82 +/- 7 nM as measured by fluorescence anisotropy (FA).

157 We developed a rapid microplate-based fluorescence anisotropy (FA)/fluorescence polarization a

158 Structural studies using fluorescence anisotropy, fluorescence correlation spectr

159 by simultaneous readout of their brightness, fluorescence anisotropy, fluorescence lifetime, and emis

160 t fluorescence spectroscopic methods such as fluorescence anisotropy, fluorescence lifetimes and fluo

161 ucted a combined measurement of stopped-flow fluorescence anisotropy, fluorescence resonance energy t

162 Fluorescence anisotropy (fluorophore-tagged analogue exc

163 imentation boundary and by the relaxation of fluorescence anisotropy following rapid dilution of labe

164 actions were characterized via bead binding, fluorescence anisotropy, gel shift, and analytical ultra

165 Fluorescence anisotropy has been used to monitor the eff

166 Fluorescence anisotropy imaging and photobleaching exper

167 n of biomolecular interactions and establish fluorescence anisotropy imaging as a quantitative techni

168 Here we advance time-resolved fluorescence anisotropy imaging combined with two-photon

169 Using two-photon fluorescence anisotropy imaging of actin-GFP, we have de

170 Our fluorescence anisotropy imaging provides an efficient wa

171 In this paper, we have developed a fluorescence anisotropy imaging system for monitoring DN

172 ignaling intermediates using high-resolution fluorescence anisotropy imaging.

173 d methods, fluorescence lifetime imaging and fluorescence anisotropy imaging.

174 mase/single-stranded DNA binding followed by fluorescence anisotropy implicated a 6:1 stoichiometry.

175 labeled aptamers to SSB governed a very high fluorescence anisotropy increase (in the 0.130-0.200 ran

176 Fluorescence anisotropy is also highly sensitive to depo

177 Results from fluorescence anisotropy, isothermal titration calorimetr

178 The potential of laser-induced fluorescence anisotropy (LIFA) with CE to characterize i

179 xperimental approaches, including gel shift, fluorescence anisotropy, light scattering, and fluoresce

180 closan in vitro on the basis of steady-state fluorescence anisotropy, light scattering, and generaliz

181 atures and analyzed the membrane fluidity by fluorescence anisotropy measurement.

182 human LysRS using affinity pull-down assays, fluorescence anisotropy measurements and gel chromatogra

183 Fluorescence anisotropy measurements and the crystal str

184 Fluorescence anisotropy measurements indicated that MinC

185 Fluorescence anisotropy measurements indicated that sept

186 In this report, a method to multiplex fluorescence anisotropy measurements is described using

187 Time-resolved fluorescence anisotropy measurements of probes covalentl

188 Fluorescence anisotropy measurements of reagents compart

189 bright internal label in microscopy, and for fluorescence anisotropy measurements of RNA dynamics.

190 array of fluorescence techniques, including fluorescence anisotropy measurements of TMA-DPH anchored

191 Analysis of the binding of DNA to p58C by fluorescence anisotropy measurements revealed a strong p

192 Steady-state and time-resolved fluorescence anisotropy measurements show that the motio

193 Finally, fluorescence anisotropy measurements showed that the S14

194 Steady-state fluorescence anisotropy measurements suggest that glycop

195 structural changes, we used frequency domain fluorescence anisotropy measurements to assess the struc

196 In this study, time-resolved fluorescence anisotropy measurements were employed to de

197 The fluorescence anisotropy measurements were examined under

198 Time-resolved fluorescence anisotropy measurements were used to provid

199 Time-resolved fluorescence anisotropy measurements with the single-Trp

200 Fluorescence anisotropy measurements yielded an equilibr

201 T binding, tyrosine intrinsic fluorescence, fluorescence anisotropy measurements, and solid-state NM

202 all angle X-ray scattering and time-resolved fluorescence anisotropy measurements, supports a sequent

203 nce recovery after photobleaching (FRAP) and fluorescence anisotropy measurements, that formation of

204 single tryptophan variants and time-resolved fluorescence anisotropy measurements, we determined that

205 ing constants in solution were obtained from fluorescence anisotropy measurements.

206 ydrolase have been detected by time-resolved fluorescence anisotropy measurements.

207 tion conditions is evaluated by steady-state fluorescence anisotropy measurements.

208 Steady-state and time-resolved fluorescence anisotropy methods applied to an extrinsic

209 Analytical ultracentrifugation and fluorescence anisotropy methods have been used to measur

210 Analytical ultracentrifugation and fluorescence anisotropy methods show that the functional

211 n studied in solution using a combination of fluorescence anisotropy, microcalorimetry, and CD titrat

212 describe a strategy using a high throughput fluorescence anisotropy microplate assay to identify sma

213 a new application of our recently described fluorescence anisotropy microplate assay to investigate

214 col for simultaneous dual-channel two-photon fluorescence anisotropy microscopy acquisition to perfor

215 Here, we present a multiphoton fluorescence anisotropy microscopy live cell imaging tec

216 Furthermore, fluorescence anisotropy nanodisc assays revealed a direc

217 lin using analytical ultracentrifugation and fluorescence anisotropy, observing tubulin in virtually

218 Steady-state fluorescence anisotropies of intermediates indicate that

219 od to image simultaneously the positions and fluorescence anisotropies of large numbers of single mol

220 Time-resolved fluorescence anisotropy of 1 in cells confirms insignifi

221 TG2 activity by following an increase in the fluorescence anisotropy of a fluorescein-labeled substra

222 ith free tryptophan, as well as the decay of fluorescence anisotropy of a labeled protein.

223 raction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane ster

224 in vitro detection of organoarsenicals using fluorescence anisotropy of ArsR-DNA interactions.

225 One-photon, time-resolved fluorescence anisotropy of Bdp-Chol decays as a triexpon

226 ined when homo-FRET is measured by decreased fluorescence anisotropy of DiI-C16.

227 der, which is derived from the time-resolved fluorescence anisotropy of DPH.

228 ith its realization based on a dependence of fluorescence anisotropy of dye molecules on heat emissio

229 No change in fluorescence anisotropy of either TMA-DPH or DPH was obs

230 based on fluorescence lifetime of LHCII and fluorescence anisotropy of erythrosine shows a high rate

231 fibrils measured by dye-release experiments, fluorescence anisotropy of labeled lipid, and confocal a

232 ein S specifically and saturably altered the fluorescence anisotropy of PC/PS-bound active site-label

233 We measured in-gel fluorescence anisotropy of phospholamban (PLB) labeled w

234 Similarly, the fluorescence anisotropy of the 1,5-IAEDANS-labeled A62C-

235 ability and differences in the steady-state fluorescence anisotropy of the enantiomers measured unde

236 Dynamics were assayed by fluorescence anisotropy of the fluorescent base analogue

237 The fluorescence anisotropy of the polypeptide upon CaM titr

238 By tracking the fluorescence anisotropy of this ligand, the ranges of Co

239 rties of these protein-protein interactions, fluorescence anisotropy of TRITC-labeled Max was used.

240 Here we report that the fluorescence anisotropy of YFP 10C depends on protein co

241 ccurs within 200 fs, resulting in a negative fluorescence anisotropy on excitation at 742 nm.

242 aracterization of hydrodynamic properties by fluorescence anisotropy or analytical ultracentrifugatio

243 We have developed a fluorescence anisotropy peptide probe using a geneticall

244 These results also demonstrate that a fluorescence anisotropy probe incorporated into a specif

245 rrelates well with the analysis based on the fluorescence anisotropy profile.

246 Fluorescence anisotropy results on variants of eotaxin-1

247 utathione S-transferase pull-down as well as fluorescence anisotropy results revealed that the NS3 pr

248 retention assays and binding measurement by fluorescence anisotropy reveal a heretofore unknown pref

249 Time-resolved fluorescence anisotropy revealed a significant decrease

250 Additionally, fluorescence anisotropy revealed that DCA causes a decre

251 Fluorescence anisotropy reveals greater than 200-fold hi

252 Conversely, quantitative fluorescence anisotropy RNA binding assays and isotherma

253 We have developed a high-throughput fluorescence anisotropy screen, using a 384-well format,

254 Using fluorescence anisotropy, SHAPE (selective 2'-hydroxyl ac

255 Stopped-flow FRET and fluorescence anisotropy show that complementary RNAs tra

256 Further experiments using fluorescence anisotropy showed a 10-fold reduction in RN

257 Although, fluorescence anisotropy showed that A3G had similar nano

258 his sensing platform allowed generation of a fluorescence anisotropy signal for aptamer probes which

259 n counting histogram analysis, time-resolved fluorescence anisotropy, single-molecule tracking, and s

260 ar dichroism, fluorescence and time-resolved fluorescence anisotropy spectroscopy.

261 Likewise, fluorescence anisotropy studies and binding studies with

262 ter interface monolayer surface pressure and fluorescence anisotropy studies reveal that the membrane

263 Fluorescence anisotropy studies showed that loss of CIP4

264 ding affinity of 3.6 microM as determined by fluorescence anisotropy studies.

265 This study was designed to use fluorescence anisotropy techniques to acquire steady-sta

266 Based on fluorescence anisotropy, the NR2B subunit binds to the f

267 Fluorescence anisotropy titration experiments showed tha

268 been performed using fluorescence intensity, fluorescence anisotropy titration, and fluorescence reso

269 ethylated versus non-methylated sequences by fluorescence anisotropy titration.

270 Fluorescence anisotropy titrations reveal that KF binds

271 Fluorescence anisotropy titrations utilizing tC as a rep

272 us p53 DNA-binding sequences using automated fluorescence anisotropy titrations.

273 Earlier studies in our laboratory have shown fluorescence anisotropy to be an effective tool in evalu

274 nd its consequent energy migration cause the fluorescence anisotropy to decrease as the number of lik

275 We used chemical dimerizers and fluorescence anisotropy to generate and visualize specif

276 Once trapped, we recorded the fluorescence anisotropy to investigate the diversity of

277 ng polymers, we took advantage of this large fluorescence anisotropy to make polarization-sensitive n

278 We have used fluorescence anisotropy to measure protein-protein and p

279 Using fluorescence anisotropy to obtain quantitative data, we

280 ircular dichroism, fluorescein emission, and fluorescence anisotropy to study the interaction between

281 Here, we used NMR and fluorescence anisotropy to study the interaction between

282 ve reconstituted the human 43 S PIC and used fluorescence anisotropy to systematically measure the af

283 (such as FRET, correlation spectroscopy, and fluorescence anisotropy) to monitor TRPV1 aggregation st

284 Using fluorescence anisotropy, TonB(33-239) was found to bind

285 Time-resolved fluorescence anisotropy (TRFA) has a rich history in eva

286 (1)H-DOSY NMR spectroscopy and time-resolved fluorescence anisotropy (TRFA) measurements.

287 energy transfer (trFRET), and time-resolved fluorescence anisotropy (trFLAN) have been used to direc

288 hat, unlike small sized rigid molecules, the fluorescence anisotropy value of the free ThT in aqueous

289 he critical micelle concentration (CMC), the fluorescence anisotropy was independent of detergent con

290 Steady-state fluorescence anisotropy was used to examine the variatio

291 Fluorescence anisotropy was used to study the protein-pr

292 Binding to oligonucleotides, examined by fluorescence anisotropy, was positively cooperative and

293 Using fluorescence anisotropy, we determined a dissociation co

294 ce emission spectrum of ECD2 polypeptide and fluorescence anisotropy, we have demonstrated that this

295 ng electrophoretic mobility shift assays and fluorescence anisotropy, we report that CPSF30 selective

296 Here, using fluorescence anisotropy, we report that TRIP8b binding t

297 Using semi-native gels and fluorescence anisotropy, we show that Hfq undergoes a co

298 s and their intended targets, measured using fluorescence anisotropy, were also highly correlated wit

299 And we report for the first time the use of fluorescence anisotropy with intact human topoisomerase

300 nalysis combining sedimentation velocity and fluorescence anisotropy yielded Kd = 84 (54-123) nm Dime