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1 cence emission, high quantum yield, and long fluorescence lifetime.
2 of serine/threonine protein kinases based on fluorescence lifetime.
3 ts ((13)C-AKIE) and decrease of 4-Cl-aniline fluorescence lifetimes.
4 ed substantial intermolecular variability in fluorescence lifetimes.
5 nd detected with on-the-fly determination of fluorescence lifetimes.
6 in larger emission quantum yields and longer fluorescence lifetimes.
7 mbrane-associated species by their differing fluorescence lifetimes.
8 and GAF show blue-shifted emission with long fluorescence lifetimes.
9 rinsic fluorophores, based on their distinct fluorescence lifetimes.
11 e, excitation intensity, and geometry, makes fluorescence lifetime a practical alternative to the est
12 ssay based on detection of changes in global fluorescence lifetime above a gold substrate, with super
13 The instrument is capable of near real-time fluorescence lifetime acquisition in multiple spectral b
14 concentration information is encoded in the fluorescence lifetime across multiple spectral bands.
20 insertion into live cell membranes, the GPs' fluorescence lifetime and diffusion time were measured i
21 ication of the phasor approach to study NADH fluorescence lifetime and emission allowed us to identif
26 urement that captures information about both fluorescence lifetime and spatial position of the probes
28 T spectroscopy that includes measurements of fluorescence lifetime and two- and three-color FRET effi
29 pic methods such as fluorescence anisotropy, fluorescence lifetimes and fluorescence quenching measur
32 steady-state fluorescence, phosphorescence, fluorescence lifetime, and phosphorescence lifetime meas
33 tween phycobilisome components, (ii) shorter fluorescence lifetimes, and (iii) red shift in the emiss
34 alize AuNPs with CdTe/CdS QDs to modulate QD fluorescence lifetimes, and nucleate the formation of fl
36 arboxyl terminal of [Ru(bpy)(2)PICH(2)](2+) (fluorescence lifetime approximately 682+/-5 ns) dye was
40 hnique to measure the changes in chlorophyll fluorescence lifetime as photosynthetic organisms adapt
41 g a large emission cross-section and a short fluorescence lifetime as the gain medium, a stable LGS Q
43 e have designed a cell-permeable T-sensitive fluorescence lifetime-based nanoprobe based on lipophili
44 his article describes novel data analysis of fluorescence lifetime-based protein kinase assays to ide
45 and mechanism of leakage is measured by the fluorescence lifetime-based vesicle leakage assay using
50 e amplitudes of 65 ps and 305 ps chlorophyll fluorescence lifetime components that was reversed after
52 rate for the first time the applicability of fluorescence lifetime correlation spectroscopy (FLCS) to
53 nd that DSBNI aggregation leads to increased fluorescence lifetimes, coupled with hypsochromic shifts
54 EGFR neutralizing antibody was confirmed by fluorescence lifetime cross-correlation measurements and
56 ate-limiting step, fitting kinetic models to fluorescence lifetime data cannot be used to derive mech
63 joint distribution of FRET efficiencies and fluorescence lifetimes determined from bins (or bursts)
68 rrelated single-photon counting, we measured fluorescence lifetimes for all CaRubies and demonstrate
69 myosins tested, we found two populations of fluorescence lifetimes for individual myosin molecules,
71 ave a large fluorescence quantum yield, long fluorescence lifetime, good photostability, and an emiss
73 spectrometry, immunoelectron microscopy and fluorescence lifetime image microscopy based on Forster
74 ual colour FLIM method we are able to detect fluorescence lifetime images of two donors to simultaneo
75 to perturb NAD(P)H metabolism, we find that fluorescence lifetime imaging (FLIM) differentiates quan
77 mer to its micelle displays that time-domain fluorescence lifetime imaging (FLIM) is able to rapidly
79 ascular ultrasound (IVUS) with multispectral fluorescence lifetime imaging (FLIm) that enables label-
87 resonance energy transfer (FRET) measured by fluorescence lifetime imaging (FLIM-FRET) and identified
88 eveloped to perform quantitative macroscopic fluorescence lifetime imaging (MFLI) over a large field
89 uscle segment length) were synchronized with fluorescence lifetime imaging and force measurements to
90 fields of biomedical sensors, spectroscopy, fluorescence lifetime imaging and in the design of many
92 eport, we apply the phasor representation of fluorescence lifetime imaging data to the quantitative s
94 , and fluorescence resonance energy transfer-fluorescence lifetime imaging experiments revealed direc
98 y simultaneous use of the phasor approach to fluorescence lifetime imaging microscopy (FLIM) and cros
100 -factors NADH and FAD with quantitation from Fluorescence Lifetime Imaging Microscopy (FLIM) as a mea
105 phases could be imaged with high contrast by fluorescence lifetime imaging microscopy (FLIM) on giant
106 Subsequently, we evaluated the probe and fluorescence lifetime imaging microscopy (FLIM) techniqu
108 rescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image
109 l two-photon microscopy and frequency-domain fluorescence lifetime imaging microscopy (FLIM) to map c
113 enerate efficient FRET, and steady-state and fluorescence lifetime imaging microscopy (FLIM) were use
114 ed the fluorescence decay of anthocyanins by fluorescence lifetime imaging microscopy (FLIM), in both
116 with fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) and
117 ed a novel Forster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM)-bas
118 e, we used Forster resonance energy transfer/fluorescence lifetime imaging microscopy (FRET/FLIM) com
119 o, by fluorescence resonance energy transfer/fluorescence lifetime imaging microscopy (FRET/FLIM), th
122 rmore, our Forster resonance energy transfer-fluorescence lifetime imaging microscopy analysis indica
125 ergy transfer-based system using multiphoton fluorescence lifetime imaging microscopy and its applica
126 scopy techniques, including FRET detected by fluorescence lifetime imaging microscopy and single-cell
129 escence resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy between fluores
130 scence biomarkers and the phasor approach to fluorescence lifetime imaging microscopy in conjunction
132 des an additional channel for multiparameter fluorescence lifetime imaging microscopy of green fluore
137 g the fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy technique.
138 excitation-Forster resonance energy transfer-fluorescence lifetime imaging microscopy to determine th
142 , using fluorescence confocal microscopy and fluorescence lifetime imaging microscopy with the phasor
145 raction with beta2 integrins, as revealed by fluorescence lifetime imaging microscopy, leading to int
148 r aggregates of LHCII-HL have been shown, by fluorescence lifetime imaging microscopy, to be particul
151 ransfer-based sensor for TrkB and two-photon fluorescence lifetime imaging microscopy, we monitor Trk
152 Along with cell cycle progression, utilizing fluorescence lifetime imaging microscopy-based Forster r
154 ing a fluorescence resonance energy transfer/fluorescence lifetime imaging microscopy-based Ras imagi
156 vity at a subcellular level using FLIM-FRET (fluorescence lifetime imaging microscopy-fluorescence re
157 minescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence re
167 expressed in N. benthamiana leaf tissues and fluorescence lifetime imaging microscopy/Forster resonan
168 myces cerevisiae) two-hybrid analysis and by fluorescence lifetime imaging microscopy/Forster resonan
169 identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidatio
171 sfunction and oxidative stress determined by fluorescence lifetime imaging of NADH and kidney fibrosi
174 this study was to investigate the benefit of fluorescence lifetime imaging ophthalmoscopy (FLIO) for
178 its activity directly in single cells using fluorescence lifetime imaging to detect Forster resonanc
179 by fluorescence resonance energy transfer by fluorescence lifetime imaging to interact directly with
182 les a diverse range of applications, such as fluorescence lifetime imaging, time-of-flight depth imag
184 ed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosom
185 ty in visualizing intracellular processes by fluorescence-lifetime imaging microscopy (FLIM) measurem
186 or can be used to image PI4KB in cells using fluorescence-lifetime imaging microscopy (FLIM) microsco
187 ally, fluorescence-resonance energy transfer-fluorescence-lifetime imaging microscopy experiments ind
188 arbons on the external surface, evidenced by fluorescence-lifetime imaging microscopy, are principall
189 study addressed this requirement by joining fluorescence-lifetime imaging microscopy/phasor multipho
190 n reaction,(13)C-nuclear magnetic resonance, fluorescence-lifetime imaging, mass spectrometry-based m
193 GFP and EGFP can be clearly distinguished by fluorescence lifetime in various models, including mamma
196 f 7-aminocoumarin dyes that have distinctive fluorescence lifetimes in different solvation environmen
197 by Zn(2+) and Cd(2+) ions inside MOFs shows fluorescence lifetimes in line with those of close-packe
199 model that sets constraints on the values of fluorescence lifetimes in the time responses of the assa
200 ers were moved from relaxation to rigor, the fluorescence lifetime increased for all label positions.
201 Measurements of pigments and chlorophyll fluorescence lifetimes indicated that the additional NPQ
203 ore the possibilities of using the available fluorescence lifetime information in PIE-FI experiments.
206 " embedded in the microbubble surface, whose fluorescence lifetime is directly related to the viscosi
208 developed method, information about analyte fluorescence lifetimes is collected by time-correlated s
211 tly labeled drugs, using fluorophores with a fluorescence lifetime larger than the rotational correla
212 n of Acd into proteins, using changes in Acd fluorescence lifetimes, Mcm/Acd FRET, or energy transfer
218 is the demonstration of how high-throughput fluorescence lifetime measurements correlate well to cha
221 An understanding of the basic physics of fluorescence lifetime measurements is required to use th
226 ic methods listed above and by time-resolved fluorescence lifetime measurements using a complementary
228 ques (UV, both steady state fluorescence and fluorescence lifetime measurements, circular dichroism (
231 optical biomarker and the phasor approach to Fluorescence Lifetime microscopy (FLIM) we identify cell
233 nfirmed by Forster-resonance energy transfer fluorescence lifetime microscopy in Arabidopsis thaliana
235 phoresis, quantitative mass spectrometry and fluorescence lifetime microscopy to characterise a serie
236 vel microscopic technique, comparable to the fluorescence lifetime microscopy, enables its applicatio
237 e to be the first near-infrared pH-sensitive fluorescence lifetime molecular probe suitable for biolo
238 -pull probes with the mechanosensitivity and fluorescence lifetime needed for practical use in biolog
240 er by biophysical studies: the excited-state fluorescence lifetime of a complex between ReAsH and a p
241 onitored by atomic force microscopy, and the fluorescence lifetime of Alexa-labeled Tau (time-correla
244 phagosomes become acidified and the average fluorescence lifetime of EGFP is known to be affected by
245 FWPV; an avipoxvirus), and the excited-state fluorescence lifetime of EGFP was reduced from 2.5 +/- 0
248 the simultaneously recorded images based on fluorescence lifetime of LHCII and fluorescence anisotro
251 H changes as measured using the pH-dependent fluorescence lifetime of SNARF-1 conjugated to urease we
255 and an iron(III) chelate that modulates the fluorescence lifetime of the peptide only when it is pho
258 EN1 (hFEN1) in the low-energy CD spectra and fluorescence lifetimes of 2-aminopurine in substrates an
260 al is found to be 6.7% and, coupled with the fluorescence lifetime on the millisecond time scale, beg
262 or complexes, based on analysis of the donor fluorescence lifetime or the spectrally resolved fluores
264 lifetime as measured in a recently developed fluorescence lifetime plate reader (Edinburgh Instrument
266 ty of responding to low pH by decreasing the fluorescence lifetime, present in the wild-type protein,
268 are combined to correlate the dependence of fluorescence lifetime reduction on the spectral overlap
269 narrow defined tunable emission peak, longer fluorescence lifetime, resistance to photobleaching and
270 We applied this technique to measure the fluorescence lifetimes responsible for the predominant,
273 itting differential scanning calorimetry and fluorescence lifetime spectroscopy denaturation data, an
274 hermore, we compare calcium titrations using fluorescence lifetime spectroscopy with the ratiometric
276 inding isotope effects (BIEs), time-resolved fluorescence lifetimes, Stokes shifts, and extended grap
278 e oxidized materials, and an increase in the fluorescence lifetime (tau(F)), due to a decrease in the
280 hin plant cells, the amplitude-weighted mean fluorescence lifetime (taum ) correlated with distinct s
281 how in response to doxorubicin, NAD(P)H mean fluorescence lifetime (taum) and enzyme-bound (a2%) frac
283 bility, together with inherent advantages of fluorescence lifetime technology (FLT) as a homogeneous,
285 they are used to measure the quenched donor fluorescence lifetime that results from Forster resonanc
286 frared region (730 nm) is observed with long fluorescence lifetimes that range from 30 to 860 ns, dep
287 which exploits high sensitivity of the OGB-1 fluorescence lifetime to nanomolar Ca(2+) concentration
288 , a technique which utilizes a fluorophore's fluorescence lifetime to probe changes in its environmen
290 he CH3NH3SnI3 film effectively increases the fluorescence lifetime up to 10 times and gives diffusion
291 , followed by measurement and imaging of the fluorescence lifetime using multiphoton excitation.
293 presentation to analyze changes in Laurdan's fluorescence lifetime we obtain two different phasor tra
294 th a time constant >10 ns, comparable to the fluorescence lifetime, we used electron spin resonance s
295 progressive internalization of EGFP-E. coli, fluorescence lifetimes were acquired and compared to con
296 ncreases from 0.22 to 0.96) while long-lived fluorescence lifetimes were observed between 1.8-2.4 ns.
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