ライフサイエンスコーパス: fluorescence polarization

1 demonstrated by oligonucleotide pulldown and fluorescence polarization.

2 s, which are then retested and quantified by fluorescence polarization.

3 and SNAP-25B at the membrane, as measured by fluorescence polarization.

4 e release of tracer results in a decrease in fluorescence polarization.

5 king of early binding events in real time by fluorescence polarization.

6 l assay for RecA-DNA filament assembly using fluorescence polarization.

7 ) for all 520 PDZ-peptide combinations using fluorescence polarization.

8 600 nM was measured by intrinsic tryptophan fluorescence polarization.

9 en DNA and the NPC fibrils was observed with fluorescence polarization.

10 he RmFixL-RmFixJ complex was investigated by fluorescence polarization.

11 rates with membrane fluidity as measured by fluorescence polarization.

12 nt peptide 1 and the alanine analogues using fluorescence polarization.

13 es for compounds which significantly reduced fluorescence polarization.

14 haracterization of binding by measurement of fluorescence polarization.

15 in was realized by measuring the decrease in fluorescence polarization.

16 nt sequence was quantitatively studied using fluorescence polarization.

17 ly higher degrees of fluidity as assessed by fluorescence polarization.

18 acids such as polyarginine and polylysine by fluorescence polarization.

19 le experimental setup and in real time using fluorescence polarization.

20 eractions with surface plasmon resonance and fluorescence polarization.

21 protease cleavage reaction was monitored by fluorescence polarization.

22 Here, we use a fluorescence polarization activity-based protein profili

23 With this goal in mind, we performed a fluorescence polarization activity-based protein profili

24 Here, we describe the development of a fluorescence polarization activity-based protein profili

25 We recently reported a fluorescence polarization-activity-based protein profili

26 We show that PME-1 is assayable by fluorescence polarization-activity-based protein profili

27 This change in fluorescence polarization allows an assay, termed DARET

28 Detection by fluorescence polarization allows real-time measurement o

29 Detection by fluorescence polarization allows real-time measurement o

30 Fluorescence polarization analysis indicated that the vI

31 Affinity was quantified by fluorescence polarization analysis of a fluorescein-tagg

32 Fluorescence polarization analysis revealed that the wil

33 polymer (MIP) synthetic antibody mimic, and fluorescence polarization analysis, for the direct detec

34 Fluorescence polarization and active site labeling, with

35 In addition, fluorescence polarization and binding assays suggest tha

36 Fluorescence polarization and competitive enzyme-linked

37 According to fluorescence polarization and dot blot analysis of synth

38 Using fluorescence polarization and electrophoretic mobility s

39 Fluorescence polarization and enzyme-linked immunosorben

40 A combination of fluorescence polarization and fluorescence resonance ene

41 Rather, fluorescence polarization and gel mobility shift experim

42 ng affinity to biotin-4-fluorescein (B4F) by fluorescence polarization and intensity measurements.

43 Here, we describe fluorescence polarization and isothermal titration calor

44 ghly restricted, in agreement with data from fluorescence polarization and NMR relaxation studies.

45 Here we use analytical ultracentrifugation, fluorescence polarization and NMR-based enzymatic assays

46 Fluorescence polarization and solid-state assays indicat

47 or high-throughput screening assays based on fluorescence polarization and strategies for assay devel

48 Fluorescence polarization and surface plasmon resonance

49 DNA-binding site preferences in vitro using fluorescence polarization and thermal denaturation profi

50 Fluorescence polarization and this model were used to ch

51 Biophysical measurements using fluorescence polarization and two-dimensional NMR implic

52 a quantitative E2-E3 binding assay based on fluorescence polarization and used this assay to measure

53 Potency of ligands was determined by fluorescence polarization and/or isothermal titration ca

54 d biochemically through affinity pull downs, fluorescence polarization, and histone reader specificit

55 ide ligands using surface plasmon resonance, fluorescence polarization, and NMR and CD spectroscopic

56 Here, we use circular dichroism, fluorescence polarization, and NMR to demonstrate the pr

57 Using a combination of fluorescence polarization- and co-immunoprecipitation-ba

58 Time-resolved fluorescence polarization anisotropy (FPA) measurements

59 a simple relationship between the actin-GFP fluorescence polarization anisotropy and the actin polym

60 Here, we introduce a powerful fluorescence polarization anisotropy approach that utili

61 fluorescence lifetime imaging microscopy and fluorescence polarization anisotropy imaging, with compa

62 Fluorescence resonance energy transfer and fluorescence polarization anisotropy studies revealed th

63 Fluorescence polarization anisotropy was used for solid-

64 he p40 subunit of the Arp2/3 complex and, by fluorescence polarization anisotropy, the binding of act

65 tration (CMC) of various detergents based on fluorescence polarization (anisotropy) of the lipophilic

66 first real-time RNase P activity assay using fluorescence polarization/anisotropy (FP/FA) with a 5' e

67 Here, we describe a new fluorescence polarization aptamer assay (FPAA) strategy

68 t since fluorescent techniques like FRET and fluorescence polarization are now commonly used in prote

69 rent cELISA, an indirect ELISA (iELISA), and fluorescence polarization assay (FPA).

70 in and extracts of cultured keratinocytes by fluorescence polarization assay against fluorogenic subs

71 Fluorescence polarization assay and molecular modeling s

72 low nanomolar binding affinity for FimH in a fluorescence polarization assay and submicromolar cellul

73 Here we report the development of a fluorescence polarization assay based on the binding to

74 nhibitors, we have developed and optimized a fluorescence polarization assay based on the interaction

75 d sarcosine scans coupled with a competitive fluorescence polarization assay developed for identifyin

76 led Forster resonance energy transfer (FRET)-fluorescence polarization assay for botulinum neurotoxin

77 evelopment of a homogeneous, high-throughput fluorescence polarization assay for identifying compound

78 The probe was successfully used to develop a fluorescence polarization assay for these six AAAEs, and

79 The fluorescence polarization assay holds promise for the di

80 An in vitro fluorescence polarization assay identified point mutatio

81 development of a competitive high-throughput fluorescence polarization assay in a 384-well format to

82 Fluorescence polarization assay reveals that BXI-61 and

83 A fluorescence polarization assay showed that LL-37 was ab

84 A fluorescence polarization assay shows that Hsp90 (MEEVD

85 blocked activation of LXR, as reflected by a fluorescence polarization assay that measures ligand-dep

86 rmed the experiments using a non-radioactive fluorescence polarization assay that quantifies the degr

87 To assist this effort, we have devised a fluorescence polarization assay that quantifies the inte

88 Rgg/SHP interactions were determined using a fluorescence polarization assay that utilized FITC-label

89 icroplate-based fluorescence anisotropy (FA)/fluorescence polarization assay that works well, even wi

90 scribe the development and optimization of a fluorescence polarization assay to identify potential in

91 systematic development and optimization of a fluorescence polarization assay to identify small molecu

92 an initial hit compound, we have developed a fluorescence polarization assay to monitor binding of co

93 confirm this hypothesis by using a sensitive fluorescence polarization assay to show that both WW dom

94 We have developed a high-throughput fluorescence polarization assay utilizing a fluorescein-

95 NC-viral RNA/DNA binding, a high-throughput fluorescence polarization assay was developed and a libr

96 pes involved in CARP-1 binding with APC-2, a fluorescence polarization assay was established that ind

97 The fluorescence polarization assay was then miniaturized to

98 f nuclear magnetic resonance binding assays, fluorescence polarization assay, and computational docki

99 Using a fluorescence polarization assay, we show a high conserva

100 Fluorescence polarization assay-based high throughput sc

101 0 of 125 nM versus 290 nM for peptide 1 in a fluorescence polarization assay.

102 EEI (IC(50) = 6.5 microM), as evaluated by a fluorescence polarization assay.

103 d their K(D)'s for SLF were measured using a fluorescence polarization assay.

104 were evaluated for MDM2-p53 inhibition in a fluorescence polarization assay.

105 (LY5), was confirmed to bind to STAT3 SH2 by fluorescence polarization assay.

106 hylcytosine (glu-5-hmC) using an equilibrium fluorescence polarization assay.

107 real-time peptide association kinetics using fluorescence polarization assays and comparing the exper

108 ion, glutathione S-transferase pulldown, and fluorescence polarization assays employing synthetic BH3

109 Fluorescence polarization assays showed that a number of

110 Gel-shift and fluorescence polarization assays showed that each aptame

111 Fluorescence polarization assays were carried out to eva

112 bodies, including ELISAs, Western blots, and fluorescence polarization assays, are complex, multiple-

113 Together with sedimentation velocity and fluorescence polarization assays, the crystal structure

114 In fluorescence polarization assays, we show that BCL11A co

115 g experiments such as radioligand assays and fluorescence polarization assays.

116 ethylstat and its application as a tracer in fluorescence polarization assays.

117 ased the fluorescence intensity and level of fluorescence polarization at an excitation wavelength of

118 specific interaction, we developed a direct fluorescence polarization based method for the detection

119 An activity assay utilized fluorescence polarization, based on the binding of fluor

120 Here, we report a fluorescence polarization-based assay for human CypA tha

121 simple high-throughput screening compatible fluorescence polarization-based assay that can be used t

122 ed MIF inhibitors and their use in the first fluorescence polarization-based assay to measure the dir

123 mains, we also developed and validated a new fluorescence polarization-based assay.

124 In this work, we report a fluorescence polarization-based binding assay for determ

125 to that of the natural Smac peptide using a fluorescence polarization-based binding assay.

126 Fluorescence polarization-based experiments revealed tha

127 ed the utility of our SNP marker set and new fluorescence polarization-based genotyping methods for s

128 -ribose dimer was then utilized in a general fluorescence polarization-based PAR-protein binding assa

129 In this effort, fluorescence polarization-based screening was used to id

130 We report here a novel high-throughput fluorescence polarization binding assay and its applicat

131 used to investigate this interaction through fluorescence polarization binding assays.

132 ion-free, homogeneous, and simple to perform fluorescence polarization bioassay for paclitaxel.

133 Data from fluorescence polarization, cell-cell fusion, and viral i

134 ln-NHBn (21), which had an IC(50) of 162 nM (fluorescence polarization), compared to 290 nM for the l

135 A fluorescence polarization competition assay has been dev

136 n on ASGR binding, we tested and validated a fluorescence polarization competition binding assay.

137 Binding constants obtained from fluorescence polarization data for toxin A binding to to

138 From the fluorescence polarization data, it is estimated that one

139 With simulated fluorescence polarization data, we tested the capacity o

140 t K(D) of 1.7 x 10(-6) m, as determined from fluorescence polarization data.

141 ed an overall decrease in diphenylhexatriene fluorescence polarization (decreased molecular order) in

142 Fluorescence polarization delineated lateral breast canc

143 Fluorescence polarization detected binding between Nox4

144 cted dye-terminator incorporation assay with fluorescence polarization detection was used as a high-t

145 However, fluorescence polarization did reveal non-sequence-specif

146 tified via high-throughput screening using a fluorescence polarization displacement assay.

147 of the rBH3 peptides using NMR spectroscopy, fluorescence polarization displacement assays, and alani

148 magnetic resonance spectroscopy techniques, fluorescence polarization displacement assays, and cell-

149 Fluorescence polarization experiments establish that TAP

150 ation enables quantitative interpretation of fluorescence polarization experiments in terms of orient

151 Here, we show through fluorescence polarization experiments that cytochrome c

152 Using fluorescence polarization experiments, we demonstrate th

153 with a KD of 1.4 x 10(-7) m as determined by fluorescence polarization experiments.

154 ptides that is the electrochemical analog of fluorescence polarization (fluorescence anisotropy), a v

155 a homogeneous high-throughput assay based on fluorescence polarization for measuring the binding affi

156 resence of serum antibodies to EIAV based on fluorescence polarization (FP) (patent pending).

157 l affinity for phosphochemicals (IMAP)-based fluorescence polarization (FP) and time-resolved fluores

158 have developed and optimized a competitive, fluorescence polarization (FP) assay for accurate and ra

159 In this study, we developed a protein-RNA fluorescence polarization (FP) assay for high-throughput

160 We report the development of a fluorescence polarization (FP) assay for PBPs and "serin

161 An automated fluorescence polarization (FP) assay has been developed

162 A competitive fluorescence polarization (FP) assay has been developed

163 dy, we describe the development of a kinetic fluorescence polarization (FP) assay that enables measur

164 Here we describe the development of a fluorescence polarization (FP) assay that is amenable fo

165 a competitive, homogeneous, high-throughput fluorescence polarization (FP) assay to identify small m

166 Here we report the development of a fluorescence polarization (FP) assay using an engineered

167 A fluorescence polarization (FP) assay was developed to de

168 for fragment finding, and thermal shift and fluorescence polarization (FP) assays are used to a less

169 well format and 3-mul final volume a pair of fluorescence polarization (FP) assays using fluorescein-

170 hods were developed, including a homogeneous fluorescence polarization (FP) competition assay that re

171 Initial studies, employing a fluorescence polarization (FP) competition assay, reveal

172 IC50 = 50-100 nM) for Hsp90 as measured in a fluorescence polarization (FP) competitive binding assay

173 the solvent is held constant, the degree of fluorescence polarization (FP) detected when a fluoresce

174 This assay applies a fluorescence polarization (FP) detection method using hu

175 ing a primer extension genotyping assay with fluorescence polarization (FP) detection.

176 nal distributions and dynamics obtained from fluorescence polarization (FP) experiments on ordered sy

177 s model enzymes to evaluate the potential of fluorescence polarization (FP) for high-throughput scree

178 say for resolvase cleavage activity based on fluorescence polarization (FP) for high-throughput scree

179 A homogeneous fluorescence polarization (FP) ligand binding assay capa

180 A fluorescence polarization (FP) microplate assay suitable

181 binding- and displacement-induced changes in fluorescence polarization (FP) of fluorescent small mole

182 to target DNAs is followed by measuring the fluorescence polarization (FP) of the dyes.

183 Fluorescence polarization (FP) provides a nondisruptive

184 his approach, which is based on steady-state fluorescence polarization (FP) spectroscopy, kinetically

185 Here, we report a novel fluorescence polarization (FP) technique for examining a

186 developed a high-throughput assay that uses fluorescence polarization (FP) to distinguish the fluore

187 Here we report the use of fluorescence polarization (FP) to measure the Kd for the

188 After comparing fluorescence polarization (FP), homogeneous time-resolve

189 biochemical and biophysical methods, such as fluorescence polarization (FP), isothermal titration cal

190 Using fluorescence polarization (FP), we have demonstrated tha

191 K(d)) of the two viral proteins, measured by fluorescence polarization (FP), were similar, and both N

192 In this study, we developed a fluorescence polarization (FP)-based BCL9 binding assay.

193 ve detection of immunoglobulin E (IgE) using fluorescence polarization (FP).

194 affinity capillary electrophoresis (ACE) and fluorescence polarization (FP).

195 Fluorescence polarization(FP) and time resolved fluoresc

196 anated fibers from rabbit psoas muscle using fluorescence polarization from bifunctional rhodamine pr

197 ient in a muscle fiber allows observation of fluorescence polarization from C-term GFP-tagged HCRLC e

198 ion in the presence and absence of actin and fluorescence polarization from cross-bridges in muscle f

199 Fluorescence polarization, giving probe angle and its di

200 3 compounds using a human PKD1 (PKCmu)-based fluorescence polarization high throughput screening assa

201 ultiple methods, including pull-down assays, fluorescence polarization, hydrogen/deuterium exchange,

202 potency than that of the lead, as judged by fluorescence polarization (IC(50) values were 110 and 13

203 Wide-field fluorescence polarization images were excited at 640 nm

204 Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are complementary tech

205 However, OCT complemented fluorescence polarization imaging by facilitating cross-

206 rgins using combined dye-enhanced wide-field fluorescence polarization imaging for en face cancer mar

207 Combined PS OCT and fluorescence polarization imaging shows promise for intr

208 s and the results are in good agreement with fluorescence polarization immunoassay (FPIA) and LC-MS/M

209 ared our assay in parallel with a commercial fluorescence polarization immunoassay (FPIA) kit in dete

210 This assay compared favorably with fluorescence polarization immunoassay (FPIA) measurement

211 g detection with that in the clinically used fluorescence polarization immunoassay (FPIA).

212 The fluorescence polarization immunoassay for vancomycin det

213 A high-throughput, competitive fluorescence polarization immunoassay has been developed

214 correlate well with the values determined by fluorescence polarization immunoassay.

215 Plasma tHCY was measured by fluorescence polarization immunoassay.

216 This observation implies a new generation of fluorescence polarization immunoassays with broad applic

217 ocedures, suggesting that the application of fluorescence polarization in combination with CypA is hi

218 ocedures, suggesting that the application of fluorescence polarization in combination with recombinan

219 In addition, the intrinsic fluorescence polarization increases from 0.327 +/- 0.001

220 on with OmcA, there was a marked increase in fluorescence polarization indicating the formation of a

221 Fluorescence polarization is an effective bridge between

222 s kinetic rotating droplet electrochemistry, fluorescence polarization, isothermal titration calorime

223 We used a commercially available fluorescence polarization ligand binding assay to invest

224 We utilized fluorescence polarization measurements and hydrogen-deut

225 cein-labeled digoxigenin obtained by FCS and fluorescence polarization measurements are identical.

226 Fluorescence polarization measurements demonstrated moti

227 NMR and fluorescence polarization measurements showed that the C

228 Fluorescence polarization measurements then allow the di

229 Applying single-molecule fluorescence polarization measurements to characterize t

230 n spectroscopy together with single molecule fluorescence polarization measurements, we have determin

231 the site 2 mutant enzymes was examined using fluorescence polarization measurements.

232 The developed fluorescence polarization method screens for small molec

233 on constant between ST1 and ST1710 using the fluorescence polarization method.

234 complementary thioflavin T fluorescence and fluorescence polarization methods.

235 nochemical energy conversion, we developed a fluorescence polarization microscope that allows us to o

236 We built an instantaneous fluorescence polarization microscope, which simultaneous

237 ue, here we use ensemble and single molecule fluorescence polarization microscopy (FPM) to determine

238 ed actin filament order in human cells using fluorescence polarization microscopy and found that clea

239 Herein, we present an excitation resolved fluorescence polarization microscopy approach to measure

240 nality, we used ensemble and single-molecule fluorescence polarization microscopy to analyze the beha

241 Our results demonstrate the ability of fluorescence polarization microscopy to measure amphipat

242 opy, leveraging molecular tension probes and fluorescence polarization microscopy to measure the magn

243 In this work, fluorescence polarization microscopy was applied to inve

244 integrin head, and measure orientation with fluorescence polarization microscopy.

245 within the nuclear pore complex (NPC) using fluorescence polarization microscopy.

246 Site-directed fluorescence polarization of 7-nitrobenz-2-oxa-1,3-diazo

247 y of the thioesterase domain, estimated from fluorescence polarization of a pyrenebutyl methylphospho

248 Statistically significant higher fluorescence polarization of cancer as compared with bot

249 The effects of sterols on the fluorescence polarization of diphenylhexatriene incorpor

250 Fluorescence polarization of membrane probes showed that

251 entation and dynamics of myosin, we measured fluorescence polarization of single molecules and ensemb

252 as model compounds, we demonstrated that the fluorescence polarization of the peptide probe increased

253 hysical effects of indomethacin, we measured fluorescence polarization of the phase-sensitive probe L

254 ramatically and differentially increased the fluorescence polarization of the phosphorylated peptide

255 ered by ethanol as monitored by steady-state fluorescence polarization of tryptophan residues.

256 te chemoreceptor, monitored the steady-state fluorescence polarization of YFP, and found that the pol

257 of protein microarrays with the fidelity of fluorescence polarization, our domain/peptide-based stra

258 of either a polarizer or analyzer alone, the fluorescence polarization ratio rises to an unexpectedly

259 Equilibrium DNA binding activity measured by fluorescence polarization reveals no difference in ssDNA

260 In a fluorescence polarization screen for the MYC-MAX interac

261 A high throughput fluorescence polarization screen that quantifies the inh

262 binatorial library synthesis and competitive fluorescence polarization screening approach that transf

263 and procedural framework for high-throughput fluorescence polarization screens to aid in this effort.

264 ncompetitive and competitive components, and fluorescence polarization shows directly that the inhibi

265 e oligonucleotide is sufficiently short, the fluorescence polarization signal can also be used to det

266 an be conveniently followed by measuring the fluorescence polarization signal in the presence of poly

267 conveniently detected by an increase in the fluorescence polarization signal of the attached fluores

268 escein-labeled c-myc peptide produced a high-fluorescence polarization signal upon binding to the c-m

269 the presence of inhibitors by monitoring the fluorescence polarization signal.

270 polyarginine and continuously measuring the fluorescence polarization signal.

271 ividual macromolecules using single molecule fluorescence polarization (SMFP) microscopy.

272 ell with results obtained by single molecule fluorescence polarization spectroscopy, indicating a lar

273 Initial in vitro fluorescence polarization studies confirmed that specifi

274 Fluorescence polarization studies indicated that apoCaM

275 Fluorescence polarization studies of small unilamellar v

276 Fluorescence polarization studies showed that CV-N is al

277 ine residues, as assumed in previous in situ fluorescence polarization studies.

278 C1 peptides using crystallographic, NMR, and fluorescence polarization studies.

279 The changing of the fluorescence polarization suggests that the motions of t

280 Combining fluorescence polarization, super-resolution microscopy,

281 n of the FKBP.rapamycin.FRB complex, we used fluorescence polarization, surface plasmon resonance, an

282 The described fluorescence polarization technique allows very low (sub

283 in of brain myosin V using a single-molecule fluorescence polarization technique that determines the

284 ding to mass spectrometry, Western blot, and fluorescence polarization, the short, proline-rich pepti

285 We further developed an assay based on fluorescence polarization to assess the mechanism of the

286 We used protein microarrays and quantitative fluorescence polarization to characterize the binding se

287 R spectroscopy, biolayer interferometry, and fluorescence polarization to characterize the Hsc70-CHIP

288 sensitive biochemical assay that relies upon fluorescence polarization to indicate peptide interactio

289 This peptide had a six fold increase in fluorescence polarization upon binding to ZipA(185-328)

290 The change of fluorescence polarization upon duplex formation inversel

291 es, which reveal fluorescence enhancement or fluorescence polarization upon their binding to DNA and

292 These behaviors were confirmed by fluorescence polarization using TAMRA-labeled peptide.

293 d enzyme concentration-dependent decrease in fluorescence polarization was observed in the COMT assay

294 Fluorescence polarization was used to determine that a 1

295 f surface plasmon resonance spectroscopy and fluorescence polarization, we determine the dissociation

296 eled DNA, and following the changes in their fluorescence polarization, we found direct evidence that

297 eous high-throughput assays, AlphaScreen and fluorescence polarization, were established to study bet

298 Using fluorescence polarization, which reflects the mobility o

299 ted for tubulin binding, causing a change in fluorescence polarization, which was an inverse function

300 fied to remove excess primer, or by means of fluorescence polarization without any additional cleanup