ライフサイエンスコーパス: fluorochrome

1 e for uniform labeling with a thiol-reactive fluorochrome.

2 ecule, thereby releasing previously quenched fluorochrome.

3 at terminates in a 7-amino-4-methyl-coumarin fluorochrome.

4 (AML) cells, all with the same near-infrared fluorochrome.

5 y, and each pool was labeled with a specific fluorochrome.

6 (6-methoxy-8-quinolyl)-p-toluene sulfonamide fluorochrome.

7 in the staining characteristics of the three fluorochromes.

8 dead yeast cells, which nonspecifically bind fluorochromes.

9 oncatameric DNA probe labelled with multiple fluorochromes.

10 seradish peroxidase (WGA-HRP) and up to four fluorochromes.

11 d bacterial artificial chromosomes producing fluorochromes.

12 rimers that are 5'-end labeled with infrared fluorochromes.

13 ease in pH1, measured using the pH-sensitive fluorochrome, 2',7'-bis(carboxyethyl)-5-(6)-carboxyfluor

14 ls was measured with the cationic lipophilic fluorochrome 3,3'-dihexyloxacarbocyanine iodide [DiOC(6)

15 d to measure cells labeled with five or more fluorochromes (a practice known as polychromatic flow cy

16 odified at the 5'- or at the 3'-end with Cy5 fluorochrome (acceptor).

17 The same performance values for the fluorochrome acid-fast bacillus smear were 33, 98, 62, a

18 The approach utilizes cycles of labeled fluorochrome addition, detection to count incorporations

19 was monitored using a dansylated polymyxin B fluorochrome agent.

20 n tumors, nonsilencing siRNA tagged with the fluorochrome Alexa 555 was encapsulated into DOPC liposo

21 first converted to an intermediate bearing a fluorochrome and amine groups, then reacted with either

22 Using a calcium-sensitive fluorochrome and digital multichannel intravital microsc

23 MP-3653) represents a liposome tagged with a fluorochrome and functionalized with a PEG chain spacer

24 rypton was stained with multiple DNA-binding fluorochromes and a monoclonal anti-double-stranded DNA

25 le blocking troublesome interactions between fluorochromes and biomolecules.

26 tumors, producing images of distributions of fluorochromes and nanoparticles as well as tumor vascula

27 al and spectral measurements of a mixture of fluorochromes and the ability to differentiate relative

28 tions of a single Ab conjugated to different fluorochromes and thus in principle can be integrated in

29 ures consisting of biologics, radionuclides, fluorochromes, and CNT could be synthesized and designed

30 etic encoded tags that can be complexed with fluorochromes, and combinations of these probes are high

31 ial resolution, nanomolar sensitivity to NIR fluorochromes, and modest NIRF light attenuation through

32 ured ROI production with oxidation-sensitive fluorochromes, and monitored cell death by annexin V and

33 tion of HREC proliferation and staining with fluorochrome-annexin V showed numerous apoptotic HRECs.

34 Apoptosis was examined using fluorochrome-annexin V staining.

35 rom 63-88C3 and CAP63 labeled with different fluorochromes as positive and negative probes, respectiv

36 /7 and caspase-9 activities were measured by fluorochrome assays.

37 e fluorescence emission of the near-infrared fluorochromes attached to the N-termini of the dendritic

38 que (FMT) that enables rapid measurements of fluorochrome-based affinity tags in live xenograft model

39 ngivalis, and phagocytosis was measured in a fluorochrome-based assay by flow cytometry.

40 nd nitric oxide levels were measured using a fluorochrome-based assay.

41 (NEUT-side-fluorescence light) labeled by a fluorochrome-based polymethine reagent using a routine a

42 To demonstrate PEG-fluorochrome shielding, fluorochrome-bearing peptide probes were synthesized, th

43 Although the transfer of small fluorochromes between them was rarely observed beyond se

44 omide colorimetric assay and the DNA-binding fluorochrome bis (benzimide) trihydro-chloride (Hoechst

45 uring the experiment, the animals were given fluorochrome bone markers for histomorphometry.

46 sistant, respectively), were loaded with the fluorochrome calcein and exposed to a range of concentra

47 We demonstrate that enzyme-activatable fluorochromes can be detected with high positional accur

48 teractions, rather than synthesizing de novo fluorochromes, can yield new approaches for the design o

49 Fluoro-Jade is an anionic fluorochrome capable of selectively staining degeneratin

50 -8, -9, and -12 activities were measured by fluorochrome caspase assays.

51 ding polymerase type, buffer conditions, and fluorochrome chemistries--a rational strategy for the se

52 each tagged with a specific fluorochrome or fluorochrome combination, has resulted in the differenti

53 tibodies (anchor markers) in the appropriate fluorochrome combinations, in order to show the quantifi

54 within 10% of their mean for each individual fluorochrome concentration and were significantly distin

55 We observed at least a 10-fold increase of fluorochrome concentration in cyclophosphamide-sensitive

56 c catheter placed above wells of varying NIR fluorochrome concentration, varied markedly (>100%) with

57 Fluorochrome concentrations derived from FMT measurement

58 cant dissimilarities in signal from distinct fluorochrome concentrations.

59 y showed that the pDNA hybridized to one PNA-fluorochrome conjugate molecule.

60 sed using C3dg-biotin tetramers complexed to fluorochrome conjugated streptavidin and measured by flo

61 followed by secondary staining with anti-Ab fluorochrome-conjugated Abs to amplify fluorescence inte

62 d, and naive animals were immunostained with fluorochrome-conjugated anti-FoxP3 and anti-CD4 monoclon

63 ese rare cells is to sort cells stained with fluorochrome-conjugated antibodies into fractions, then

64 d arborization of vessels labeled in vivo by fluorochrome-conjugated lectin with effective treatment.

65 pensions obtained from gingival tissue, with fluorochrome-conjugated monoclonal antibodies against CD

66 Tie2-GFP transgenic mice in combination with fluorochrome-conjugated monoclonal antibodies to vascula

67 different concentrations of a cocktail of 4 fluorochrome-conjugated monoclonal antibodies.

68 After tumor disaggregation, staining with a fluorochrome-conjugated monoclonal antibody, and flow cy

69 Construction of fluorochrome-conjugated peptide-I-A(b) class II tetramer

70 ion of epitope-specific CD4 T cells by novel fluorochrome-conjugated peptide-I-A(b) tetramers allows

71 Fluorochrome-conjugated peptide-MHC (pMHC) class I multi

72 Fluorochrome-conjugated peptide-MHC (pMHC) multimers are

73 f SCT and AT-SCT peptide-MHC complexes using fluorochrome-conjugated TCR multimers suggests that nona

74 On unfixed borreliae, fluorochrome-conjugated uPA bound to all species.

75 CD4 with specific monoclonal antibody (MoAb)-fluorochrome conjugates and for DNA polyploidization wit

76 Fluorochrome conjugates and UV microscopy were used to q

77 fferences in monoclonal antibodies and their fluorochrome conjugates, or failure of the total absolut

78 rt a method to couple commercially available fluorochromes covalently and sequence-specifically to pD

79 the donor fluorochrome FITC and the acceptor fluorochrome Cy3.

80 pairs per millimeter and detection limit for fluorochrome Cy5.5 of 1-10 pmol.

81 The system is linear down to fluorochrome densities <<1 molecule/microm2.

82 injection of fluorescent tracer (Fluorogold; Fluorochrome, Denver, CO) into the superior colliculus t

83 loss (judged by lack of Fluorogold labeling; Fluorochrome, Denver, CO), adjacent areas with relative

84 as assessed by changes in bone geometry and fluorochrome-derived bone formation rates.

85 The single-fluorochrome detection limits achieved by the cumulative

86 ion was measured using the oxidant-sensitive fluorochrome dihydrorhodamine 123 (DHR-123).

87 dant production (using the oxidant-sensitive fluorochrome dihydrorhodamine-123) were monitored by int

88 nd 596) were labeled with a europium chelate fluorochrome (donor).

89 nced dynamic bone formation rates in in vivo fluorochrome double labeling experiments.

90 By using dual fluorochrome dyes (Hoechst and Nile Red) to locate nucle

91 retrograde labeling of RGCs with Fluorogold (Fluorochrome; Englewood, CO) was used.

92 protocol, using transgenic mouse brains with fluorochrome expression in microglia.

93 ferentiation was examined by using indicator fluorochrome-filled liposomes in an enhanced immunofluor

94 ytometric analysis of FRET between the donor fluorochrome FITC and the acceptor fluorochrome Cy3.

95 animals were stained with a newly developed fluorochrome (Fluoro-Jade) specific for degenerating neu

96 followed by staining with a newly developed fluorochrome (Fluoro-Jade) specific for degenerating neu

97 ms and the high specificity of the PicoGreen fluorochrome for double-stranded (ds)DNA.

98 cquisition of a series of images of multiple fluorochromes from multiple arrays occurs under computer

99 radiolabeled elements or sterically hindered fluorochrome groups, are usually employed to screen phos

100 emiacetal aglycon leaving group that tethers fluorochromes in close proximity, also positioning them

101 Continuous (1- to 5-minute) excitation of fluorochromes in the capillary lumen (light-dye treatmen

102 of rhodopsin's screening of autofluorescent fluorochromes in the RPE.

103 henol-glucuronide moiety (TrapG) to trap the fluorochromes in the vicinity of the active enzyme.

104 eroxidase (WGA-HRP) and up to four different fluorochromes in V2 labeled neurons and terminations in

105 p-nitrophenyl phosphonate-conjugated fluorochromes, including the very bright and stable quan

106 the 3' end of the first ODN and NIR acceptor fluorochromes (indodicarbocyanine Cy7 or, alternatively,

107 Caspase 3 activity was measured by a fluorochrome inhibitor of caspase (FLICA) assay.

108 -3, -8, and -9 activities were measured by a fluorochrome inhibitor of caspase (FLICA) assay.

109 aspase-9 and -12 activities were measured by fluorochrome inhibitor to caspase (FLICA) assays.

110 transcripts by the incorporation of a second fluorochrome into DNA templates promotes more robust and

111 Infusion of Fluo-4 AM, a calcium-sensitive fluorochrome, into the mouse circulation resulted in dye

112 nce resonance energy transfer (FRET) between fluorochromes introduced into these DNA molecules.

113 CNIR800, that carries a near-infrared (NIR) fluorochrome IRDye 800CW, with a quencher connected thro

114 dition, the assay sensitivities using direct fluorochrome label as the reporter, or alkaline phosphat

115 in that bound HLA-A*0201 were identified and fluorochrome-labeled A*0201 tetramers with each peptide

116 d-type mice by intravenous administration of fluorochrome-labeled anti-E- and anti-P-selectin antibod

117 Neutrophil phenotype was determined using fluorochrome-labeled antibodies to CD16 and CD11b and as

118 Cells were characterized using fluorochrome-labeled antibodies to surface markers, intr

119 rix is externalized and immediately bound by fluorochrome-labeled avidin present in the culture mediu

120 n in real time by exploiting the capacity of fluorochrome-labeled avidin to stain degranulating cells

121 s supported by competitive binding assays of fluorochrome-labeled beta25-35 and Mac-1 antibodies (Wt.

122 d anti-CD11b) and (2) competitive binding of fluorochrome-labeled beta25-35 with anti-CD18 or anti-CD

123 cipient origin, is combined with multi-color fluorochrome-labeled CD epitope-specific monoclonal anti

124 itopes of the target are modified with short fluorochrome-labeled complementary oligonucleotides and

125 Physical proximity between fluorochrome-labeled CR3 and Fc gamma RIIIB on individua

126 Intracellular fluorochrome-labeled diamine oxidase (DAO) was used as a

127 alpha-subunit of E. coli RNA polymerase to a fluorochrome-labeled DNA fragment containing the rrnB P1

128 logy is protein-dependent association of two fluorochrome-labeled DNA fragments, which allows generat

129 sure both snapshot and finish-line images of fluorochrome-labeled DNA.

130 ling of both fixed and live spirochetes with fluorochrome-labeled Fab-CB2 and 11G1, and an immunoglob

131 proliferation of B-1a lymphocytes that bind fluorochrome-labeled Ft-LPS and the differentiation of t

132 populations were identified by staining with fluorochrome-labeled HLA tetramers and anti-CD19, CD27,

133 ults obtained with fluorogenic substrates or fluorochrome-labeled inhibitors of caspases.

134 Fluorochrome-labeled Mabs were used in T-cell phenotypic

135 The assay uses fluorochrome-labeled monoclonal antibodies to HCMV immed

136 lymerase-mediated incorporation of different fluorochrome-labeled nucleotides (FdNTPs) was investigat

137 to mediate the consecutive incorporation of fluorochrome-labeled nucleotides into an array of large

138 t-dependent annealing of short complementary fluorochrome-labeled oligonucleotides attached to a pair

139 In vitro, PEG blocked the interactions of fluorochrome-labeled peptide probes with each other (abs

140 Upon mixing RNAP and fluorochrome-labeled promoter a time-dependent biphasic

141 tinal ganglion cell layer cells (RGCLCs) and fluorochrome-labeled retinal ganglion cells (RGCs) on fl

142 Preservation of fluorochrome-labeled RGCs was assessed 2 weeks later.

143 rs followed by intracellular staining with a fluorochrome-labeled SLP-76-specific Ab.

144 Optical imaging with a fluorochrome-labeled version of the mAb showed colocaliz

145 ive, as characterized by irregular, punctate fluorochrome labeling and elevated proteoglycan.

146 d is based on biotinylation and streptavidin-fluorochrome labeling of APCs, followed by subsequent ac

147 Fluorochrome labeling of dentin in Dmp1-null mice showed

148 Using fluorochrome labeling, the present study determined the

149 on chamber for PCR, which through the use of fluorochrome labelled probes allows the target molecules

150 Fluorochrome-labelled monoclonal antibodies were used in

151 ing propidium iodide, a nucleic acid-binding fluorochrome largely excluded by the intact cell membran

152 included donor NIR Cy5.5 indodicarbocyanine fluorochrome linked to the 3' end of the first ODN and N

153 Using PEG to block fluorochrome-mediated interactions, rather than synthesi

154 tensity into molecules of equivalent soluble fluorochrome (MESF) units.

155 tandard units of mean equivalents of soluble fluorochrome (MESF).

156 alculated as molecules of equivalent soluble fluorochrome (MESF).

157 ifferentiate relative concentrations of each fluorochrome mixture in real time.

158 ase-DNA template complex that considered the fluorochrome moiety of the FdNTPs and the linker length

159 cular imaging, generally designed by placing fluorochromes on a cleavable substrate in close proximit

160 painting probes, each tagged with a specific fluorochrome or fluorochrome combination, has resulted i

161 e C2A domain of synaptotagmin I labeled with fluorochromes or a radioisotope binds to both apoptotic

162 ng Br-RNA was immunolabelled indirectly with fluorochromes or gold particles.

163 was performed without the use of antibodies, fluorochromes, or dyes, leaving the sorted spores in the

164 technique by staining tissue specimens with fluorochrome- or biotin-labeled GAGs to visualize the di

165 A was 68,171 molecules of equivalent soluble fluorochrome (P<0.0001), representing 55%+/-22%.

166 64 to 59,753 molecules of equivalent soluble fluorochrome per cell (MESF), as determined by flow cyto

167 nucleotide probes were synthesized with five fluorochromes per molecule, and the light emitted by a s

168 ystem was validated with in vitro imaging of fluorochrome phantoms and in vivo fluorescence measureme

169 velopment and validation of simple models of fluorochrome point spread functions within the imaging s

170 graft copolymer bearing near-infrared (NIR) fluorochromes positioned on cleavable substrate sequence

171 ection depends on FRET efficacy and relative fluorochrome positions within ODN binding sites.

172 used to measure protein phosphorylation; the fluorochrome probe 2'7'-dichlorofluorescin diacetate was

173 to energy resonance transfer among the bound fluorochromes (quenching) but became brightly fluorescen

174 entary studies, nucleotides labeled with the fluorochrome R110 were incorporated into surface-elongat

175 Easy to find: magnetic nanoparticles bearing fluorochromes (red) that intercalate with DNA (green) fo

176 idin conjugated to magnetic nanoparticles or fluorochromes, respectively.

177 arrays, two triplicate hybridizations (with fluorochrome reversal) comparing RNAs from a fibroblast

178 A method to attach a fluorochrome sequence-specifically to supercoiled plasmi

179 e differential uptake of an (111)In-labeled, fluorochrome-shielded, integrin-binding RGD probe and a

180 ach for fluorescent probe design termed "PEG-fluorochrome shielding", where PEGylation enhances quant

181 To demonstrate PEG-fluorochrome shielding, fluorochrome-bearing peptide pro

182 blood compared with the intensity of the raw fluorochrome signal, which would vary +/-60%.

183 beled chromosome-painting probes with unique fluorochrome signatures onto human or mouse metaphase ch

184 polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellul

185 ealed through staining with the aniline blue fluorochrome, Sirofluor.

186 e use of recommended methods was as follows: fluorochrome stain for acid-fast bacillus microscopy (47

187 uberculosis, and 13 (38%) of these were also fluorochrome stain positive.

188 ochrome stain-positive specimens and 57% for fluorochrome stain-negative specimens.

189 LCR sensitivity was 100% for fluorochrome stain-positive specimens and 57% for fluoro

190 itutions with abnormal chest radiographs and fluorochrome stain-positive sputa were evaluated for tub

191 16S ribosomal RNA (16S rRNA) expression and fluorochrome staining with Mitotracker Red CMXRos (Delta

192 ression, live-cell imaging, and aniline blue fluorochrome staining.

193 amined the route of entry into mouse skin of fluorochrome-tagged or naked second-generation 2'-O-meth

194 The elongation products were labeled with fluorochrome-tagged probes and detected in a flow cytome

195 ice were stained with Fluoro-Jade B (FJB), a fluorochrome that binds specifically to degenerating fib

196 ligonucleotides), each of which containing a fluorochrome that can form a fluorescence resonance ener

197 staining using Abs of interest labeled with fluorochromes that are compatible with HO342 and 7-AAD e

198 tary oligonucleotides that are modified with fluorochromes that could participate in fluorescence res

199 ility of an expanded range of antibodies and fluorochromes that have improved our ability to identify

200 as engineered by conjugating a near-infrared fluorochrome to a peptide ligand derived from the amino

201 duced a third probe labeled with a different fluorochrome to create a triple-probe/three-color system

202 cular tomography and a cathepsin-activatable fluorochrome to image autophagy in the heart in vivo aft

203 NA in alkaline conditions and uses PicoGreen fluorochrome to monitor denaturation.

204 group that facilitates the attachment of the fluorochrome to nucleophilic moieties located near beta-

205 ne is reacted with a maleimide moiety on the fluorochrome to produce a fluorescent conjugate which is

206 tem was tested by using a combination of the fluorochromes to confirm spectral separation.

207 -specific coupling of commercially available fluorochromes to DNA expression vectors while retaining

208 stigated by placing restricted injections of fluorochrome tracers, wheat germ agglutinin-horseradish

209 we found that the simple addition of an anti-fluorochrome unconjugated Ab during staining resulted in

210 we attempted to improve staining using anti-fluorochrome unconjugated primary Abs followed by second

211 y hypoxic rats were in vivo-labeled with DiI fluorochrome via liposome delivery and subsequently iden

212 diolabeling ((89)Zr-DNP) and a near-infrared fluorochrome (VT680) for microscopic and cellular valida

213 In tissue, 250 pmol of fluorochrome was easily detected during the 10-second im

214 rm (containing a (18)F isotope and a far red fluorochrome), we show good correlations between FMT and

215 ious pairs of proteins fused to two distinct fluorochromes were coexpressed in N. benthamiana leaf ti

216 Both Cy7 and 800CW fluorochromes were linked by using hydrophilic internucl

217 The different fluorochromes were readily distinguished with the system

218 markers and the corresponding antibodies and fluorochromes were selected based on their contribution

219 e case of Cy5.5-Cy7 and Cy5.5-800CW pairs of fluorochromes, which was sensitive to the relative posit

220 These fluorogenic dyes integrate a coumarin fluorochrome with the bioorthogonal trans-cyclooctene(TC

221 ergo an internal charge transfer to form new fluorochromes with longer pi-electron systems.