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1 f the exogenous enzyme on the dye results in fluorogenic activation of the dye, enabling fast voltage
4 termine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluore
10 2-naphthaldehyde was highly fluorescent, the fluorogenic aldehyde, 4-formylbenzene connected to the 6
11 90 nm suggesting a possibility of developing fluorogenic aldehyde-reactive probes based on ABAO frame
13 trations of fluorescein diphosphate (FDP), a fluorogenic alkaline phosphatase substrate, with and wit
14 The method is nonenzymatic, isothermal, and fluorogenic, allowing the direct detection of nucleic ac
16 with a newly developed, extremely sensitive fluorogenic alpha-tocopherol analogue (H4BPMHC), we repo
17 rotocol covers the chemical synthesis of the fluorogenic amino acid Fmoc-Trp(C2-BODIPY)-OH (3-4 d), t
18 he preparation of the tryptophan (Trp)-based fluorogenic amino acid Fmoc-Trp(C2-BODIPY)-OH and its in
20 a set of monolignol analogs gamma-linked to fluorogenic aminocoumarin and nitrobenzofuran dyes were
21 rein the synthesis and characterization of a fluorogenic analogue of ubiquinone designed to reversibl
22 hesis, and characterization of a two-segment fluorogenic analogue of vitamin K, B-VKQ, prepared by co
25 report here the preparation of a lipophilic fluorogenic antioxidant (Mito-Bodipy-TOH) that targets t
26 findings underscore the potential of the new fluorogenic antioxidant Mito-Bodipy-TOH to study the che
27 s that function by H2S-mediated reduction of fluorogenic aryl azides has emerged as one of the most c
28 e we describe a new mix-and-read homogeneous fluorogenic assay for the measurement of the AP-site cle
29 arase enzymatic activity, we have designed a fluorogenic assay in which 7-amido-4-methyl coumaric aci
31 l data reported in this study along with the fluorogenic assay will be useful in substrate specificit
33 We previously described the development of fluorogenic assays for nucleic acid-modifying enzymes ba
34 and inexpensive platform for development of fluorogenic assays that do not require organic fluoropho
35 e capability of the system as a platform for fluorogenic assays was demonstrated through designing mo
37 ectron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversio
38 lishes a platform for the rational design of fluorogenic azide probes with spectral properties tailor
42 used to synthesize soluble and precipitating fluorogenic beta-lactam substrates that demonstrated dif
46 milieu to electrophiles, we have designed a fluorogenic BODIPY-acrolein probe, AcroB, that undergoes
47 lpains were active in vivo by microinjecting fluorogenic calpain substrates into cortical fiber cells
50 e used as a highly selective chromogenic and fluorogenic chemosensor for the detection of cyanide in
51 1947 to 2010 related to the design of chromo-fluorogenic chemosensors and reagents for explosives (14
54 vation of the fluorescence in situ through a fluorogenic conjugation step effectively constrains the
57 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorogenic core, which works as an environmentally sens
59 An RNA aptamer selected for binding to the fluorogenic cyanine dye, dimethylindole red (DIR), also
60 rrolidine-3,4-diol (APPD), for the selective fluorogenic derivatization of 3-nitrotyrosine (3-NT) res
62 te sensor provides a unique platform for the fluorogenic detection of PPi having a very low limit of
64 ndem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometri
65 mentary-readout homogeneous assays utilizing fluorogenic donor/quencher and AlphaScreen chemiluminesc
68 ds to the target of the Spinach aptamer, the fluorogenic dye DFHBI, and thereby induces a strong fluo
69 s indicate that alpha-CN-TO will be a useful fluorogenic dye in combination with specific RNA and pro
70 in (FAP) that binds a heavy atom-substituted fluorogenic dye, forming an 'on-demand' activated photos
71 irst time the application of a water-soluble fluorogenic dye, Thioflavin T (ThT), in a dual role of e
74 We have developed a class of dendron-based fluorogenic dyes (termed dyedrons) comprised of multiple
78 single-chain variable fragments and activate fluorogenic dyes, which only become fluorescent upon act
83 zymatic reactions, two new simple and robust fluorogenic enzymatic assays for MGL and AHCY were devel
84 vestigations led to the discovery of two new fluorogenic epsilon-N-succinyllysine-containing substrat
85 on embedding fluorescein dilaurate (FDL), a fluorogenic ester substrate, into the polyester matrix a
87 l applications, we have designed an infrared fluorogenic executioner-caspase reporter, which reveals
89 C-S bond coupling reaction enabled the first fluorogenic formation of phospholipids, which self-assem
90 tors had high affinity toward both synthetic fluorogenic FRET peptides (95%) and dentin powder substr
91 ic activity was determined in solution using fluorogenic gelatin and peptide substrates, by gelatin z
92 fluorescence of the immobilized TFP and the fluorogenic group provided a direct tool to distinguish
96 also demonstrate that dihydroresorufin, as a fluorogenic indicator, gives an improved temporal respon
97 ion of FEEM focused on the use of a specific fluorogenic indicator, resazurin, which is reduced to ge
98 are presented as inexpensive and impermanent fluorogenic indicators for the selective, standoff/remot
103 to develop a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to
106 such conformational changes was enabled by a fluorogenic ligand that fluoresces upon AgHalo forming s
110 is unknown, and therefore we used an in situ fluorogenic MMP sensor peptide to determine how drug tre
111 nomer 1 for the integration into chromo- and fluorogenic molecularly imprinted polymers (MIPs) was sy
112 nsidered as the brightest polarity-sensitive fluorogenic molecules reported to date, having approxima
116 method combining terminal phosphate-labeled fluorogenic nucleotides (TPLFNs) and resealable polydime
117 ion manifold after reacting with mM order of fluorogenic o-phthalaldehyde (OPA)/thiol reagent (pH 10.
118 living cells using molecular beacons (MBs) - fluorogenic oligonucleotide probes - with minimal target
123 binant TPPE was able to process the quenched fluorogenic peptide and to cleave the acidic domain at l
125 ited Bmp1 activity in vitro as measured by a fluorogenic peptide based procollagen C-proteinase activ
127 was guided by an assay that used a quenched fluorogenic peptide comprising the amino acid sequence b
128 tro that DPPI activates pro-KLK4 to cleave a fluorogenic peptide containing a KLK4 cleavage site.
132 intact but hyperactive in the hydrolysis of fluorogenic peptide substrates and the degradation of po
133 orbent Surface Area" that uses conversion of fluorogenic peptide substrates to measure the intrinsic
135 adation of other MMP-9 substrates, such as a fluorogenic peptide, alphaB crystalline, or nonmuscular
136 detailed evaluation of two prodrug-inspired fluorogenic peptides designed to be efficient and select
138 her examined using active site mutants and a fluorogenic phospholipid substrate whose hydrolysis by R
139 er describes the development of homogeneous, fluorogenic polymerase, restriction endonuclease, and li
140 nching photo-induced electron transfer (PET) fluorogenic primers for the detection of P. falciparum a
143 k capacity, and in part by time-dependent RA-fluorogenic probe conjugate degradation by cellular prot
144 In the second experimental scenario, the RA-fluorogenic probe conjugate is pre-formed in a cell by s
145 Removal of the stress results in apparent RA-fluorogenic probe conjugate re-folding, mediated in part
148 uorescein diacetate) is the most widely used fluorogenic probe for the detection of general oxidative
150 e of multiplexing may be increased by adding fluorogenic probe pairs with other emission wavelengths.
151 ction relationships in Dbr1 enzymes, and the fluorogenic probe permits high-throughput screening for
154 esults highlight the potential of B-VKQ as a fluorogenic probe to study electron transfer and transpo
158 with activity: A hydrogen sulfide selective fluorogenic probe, 7-azido-4-methylcoumarin (AzMC), serv
164 demonstrated through the preparation of two fluorogenic probes for the selective detection of biothi
165 ld, we demonstrate the ability to synthesize fluorogenic probes for the sensitive and selective detec
167 oteases were challenged to cleave bead-bound fluorogenic probes of citrulline-dependent proteolysis,
170 fferent amplification products is the use of fluorogenic probes that emit at different wavelengths (c
172 is shown that such molecules can be used as fluorogenic probes to detect the template-dependent bind
175 The design, synthesis, and evaluation of fluorogenic probes with high specificity for BlaC, a bio
176 eraction between the ROS/RNS species and the fluorogenic probes, as follows: superoxide using hydroet
182 esented here exploits the solvatochromic and fluorogenic properties of nile red to extract both the e
184 ow-based assay, which takes advantage of the fluorogenic properties of phosphorylated coumaryl amino
187 nalytical applications, such as studies with fluorogenic protease substrates or nucleic acids probes.
191 within an organelle of choice, using a fast fluorogenic reaction between a tetrazine-functionalized
193 edox reaction of interest to a complementary fluorogenic reaction converting an electrochemical signa
200 ances in using single-molecule microscopy of fluorogenic reactions to examine and understand the spat
201 eic acid probes, which show highly selective fluorogenic reactivity when annealed to their respective
203 labeling, we employ Chromeo P540, which is a fluorogenic reagent that converts cationic lysine residu
206 However, the use of DCFH2-DA, as many other fluorogenic redox probes, is mainly confined to the dete
207 tion of single redox events are the use of a fluorogenic redox species and the nanoconfinement and hi
209 coupling a conventional redox reaction to a fluorogenic reporter reaction on a closed bipolar electr
210 e this limitation, here we describe XY-69, a fluorogenic reporter that preferentially partitions into
212 describe the development and optimization of fluorogenic reporters that detect the proteolytic activi
213 at facilitates turnover amplification of the fluorogenic response in nucleic acid-templated reactions
214 Metalloporphyrin 3 shows a multiple channel fluorogenic response toward fluoride and cyanide and als
215 acid) through sharp colorimetric as well as fluorogenic responses even conspicuous with the naked ey
222 ine dye thiazole orange (TO) is a well-known fluorogenic stain for DNA and RNA, but this property pre
223 n we describe the development of a family of fluorogenic stilbene-based vinyl amides and vinyl sulfon
224 ultifaceted behavior, which depends upon the fluorogenic substituent, the solvent, and the basicity o
225 g the lysophosphatidylcholine-like substrate fluorogenic substrate 3 (FS-3) and approximately 10,000
226 iluted 20000-fold into buffer containing the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacrid
227 d with a solution containing glucose and the fluorogenic substrate amplex red through the engineered
233 plication of a novel type of chromogenic and fluorogenic substrate for protease detection is describe
234 plarily demonstrated for the cleavage of the fluorogenic substrate hydrodabcyl-Ser-Phe-EDANS by the p
237 ty was assayed by monitoring cleavage of the fluorogenic substrate Mca-RPPGFSAFK(Dnp)-OH, a derivativ
239 (Danio rerio) CYP1 isoforms with a series of fluorogenic substrate probes was determined by the rate
241 ts, containing varying concentrations of the fluorogenic substrate resorufin beta-d-galactopyranoside
242 the TF.factor VIIa (FVIIa) complex toward a fluorogenic substrate showed that the catalytic efficien
245 ed components are mixed with an enzyme and a fluorogenic substrate that permits to follow the enzymat
246 latin zymography in SDS-PAGE, and by in situ fluorogenic substrate zymography in RPE/choroid sections
247 ntained heat-labile sialidase activity for a fluorogenic substrate, 2'-(4-methylumbelliferyl)-alpha-D
248 fluorescent compounds seen with the existing fluorogenic substrate, 4-methylumbelliferyl-alpha-D-gluc
250 el in human plasma, employing ecarin and the fluorogenic substrate, resulted in improvement of the de
251 tral enzyme of this network, by developing a fluorogenic substrate-based method for time- and space-r
254 ded MBL)) and the identification of suitable fluorogenic substrates (umbelliferone-derived cephalospo
255 e expression were assessed by using specific fluorogenic substrates and macroarrays, respectively.
257 In this work, we report readily synthesized fluorogenic substrates enabling enzyme-economical evalua
258 ethylrhodamine) have been used previously as fluorogenic substrates for a number of DNA modifying enz
259 r enzyme fluorescence (REF) that uses custom fluorogenic substrates for bacterial enzymes allows rapi
260 k through development of near-infrared (NIR) fluorogenic substrates for beta-lactamase, an enzyme exp
263 e in fluorescence on caspase cleavage of the fluorogenic substrates ranged from 40 to 83 depending on
264 trates are required in costly amounts, while fluorogenic substrates require an additional apparatus (
268 n droplet microfluidic screening systems use fluorogenic substrates to measure enzymatic activities w
269 Mildly permeabilized cells were exposed to fluorogenic substrates to monitor the activity of intrac
274 ently monitored with peptidic chromogenic or fluorogenic substrates, with certain peptide sequences i
280 on of the glycans with either chromogenic or fluorogenic tags for optical measurement or isotopic tag
283 fficient labeling of apoptotic cells using a fluorogenic tetrazine dye in a pre-targeting approach.
284 e believe highly selective proximity-induced fluorogenic tetrazine reactions could find broad uses in
285 cid derivative with a ligation reaction of a fluorogenic tetrazine, allowing for the imaging of sialy
287 proteins for tracking and imaging by use of fluorogenic tetrazines that become strongly fluorescent
288 ween 7-azabenzonorbornadiene derivatives and fluorogenic tetrazines that facilitates turnover amplifi
291 tleneck and report on the development of new fluorogenic transmembrane peptide substrates, which are
294 The enzyme kinetics for hydrolysis of three fluorogenic triple-helical peptides (fTHPs) was evaluate
296 microtubules in live mammalian cells via the fluorogenic, two-photon-triggered photoclick chemistry w
297 bioprobe by integrating tetraphenylsilole, a fluorogenic unit with aggregation-induced emission (AIE)
298 The anion binding tendencies of the two fluorogenic ureas L(1)H and L(2)H, containing the 2-anth
299 neither from literature nor from results in fluorogenic vesicles and thus support the importance of
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