ライフサイエンスコーパス: fluorogenic substrate

1 5) value of 342 microM were obtained for the fluorogenic substrate.

2 red for maximal enzymatic activity against a fluorogenic substrate.

3 sured in transduced cells and media, using a fluorogenic substrate.

4 bstrate poly(ADP-ribose) polymerase and of a fluorogenic substrate.

5 focal fluorescence microscopy using a Bodipy fluorogenic substrate.

6 low micromolar inhibitory potency against a fluorogenic substrate.

7 in as the enzyme and BODIPY FL casein as the fluorogenic substrate.

8 difluoro-4-methylumbelliferyl phosphate as a fluorogenic substrate.

9 r aminopeptidase activity against many model fluorogenic substrates.

10 cted and quantitated by flow cytometry using fluorogenic substrates.

11 eactions is often limited by the need to use fluorogenic substrates.

12 human sirtuin hydrolases against a panel of fluorogenic substrates.

13 igher chymotrypsin-like activity measured on fluorogenic substrates.

14 the cleavage of macromolecular and synthetic fluorogenic substrates.

15 caspase-12 when incorporated into synthetic fluorogenic substrates.

16 iety of intracellular enzymes with available fluorogenic substrates.

17 ratio at a given analyte concentration with fluorogenic substrates.

18 inatorial substrate libraries and individual fluorogenic substrates.

19 cPLA2 showed 20-fold higher activity toward fluorogenic substrates, 1-O-(1-pyrenedecyl)-2-arachidono

20 ing enzyme (IDE)-catalyzed hydrolysis of the fluorogenic substrate 2-aminobenzoyl-GGFLRKHGQ-ethylened

21 parameters for the interaction of E with the fluorogenic substrate 2-aminobenzoyl-Thr-Ile-Nle-Phe(p-N

22 ntained heat-labile sialidase activity for a fluorogenic substrate, 2'-(4-methylumbelliferyl)-alpha-D

23 g the lysophosphatidylcholine-like substrate fluorogenic substrate 3 (FS-3) and approximately 10,000

24 fluorescent compounds seen with the existing fluorogenic substrate, 4-methylumbelliferyl-alpha-D-gluc

25 analysis of xylanase activity using a novel fluorogenic substrate, 6,8-difluoro-4-methylumbelliferyl

26 e assay measures the rate of hydrolysis of a fluorogenic substrate-6,8-difluoro-4-methylumbelliferyl

27 atalytic specificity was determined with the fluorogenic substrate: 6-carboxyfluorescein approximatel

28 the rat theta 2-2 enzyme (rGSTT2-2) with the fluorogenic substrate 7-amino-4-chloromethyl coumarin (C

29 e H(2)O(2)-supported oxidation of the marker fluorogenic substrate 7-ethoxy-4-trifluoromethylcoumarin

30 iluted 20000-fold into buffer containing the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacrid

31 continuously monitored using a validated MMP fluorogenic substrate, a microdialysis system placed wit

32 ctions of Staphylococcus aureus sortase with fluorogenic substrate Abz-LPETG-Dnp in the presence or a

33 reening of a library of 137,180 tetrapeptide fluorogenic substrates against the T. brucei 20 S protea

34 Fluorogenic substrates allow for direct measurement of e

35 The cell-permeable fluorogenic substrate allows single-cell cloning of cell

36 d with a solution containing glucose and the fluorogenic substrate amplex red through the engineered

37 ndividual reaction vessels containing excess fluorogenic substrate and catalyzed the production of a

38 Together, the fluorogenic substrates and antibody aldolases provide re

39 Di- and tetra-oligopeptides were used as fluorogenic substrates and irreversible/competitive inhi

40 e expression were assessed by using specific fluorogenic substrates and macroarrays, respectively.

41 to detect the production of thrombin using a fluorogenic substrate, and (iv) titration of argatroban

42 al-Try-7 amino-4 methyl coumarin, a specific fluorogenic substrate, and Cbz-Leu-Leu-Tyr-CHN2, a speci

43 Values of k(cat)/K(M) with hypersensitive fluorogenic substrates are 10(4) and 10(2)-fold lower th

44 The hydrolysis of a fluorogenic substrate, Arg-528/Asp-575 < Lys-528/Asp-575

45 e compared with pro-memapsin 2 using two new fluorogenic substrates, Arg-Glu(5-[(2-aminoethyl)amino]n

46 as a marker and the design of BlaC-specific fluorogenic substrates as probes for Mtb detection.

47 rance of peptidase activity that cleaved the fluorogenic substrates Asp-Glu-Val-Asp-aminotrifluoromet

48 ity was monitored in cells after PDT using a fluorogenic substrate, (Asp-Glu-Val-Asp)-AFC (7-amino-4-

49 protease activity in extracts measured by a fluorogenic substrate assay.

50 Using fluorogenic substrate assays, Western blotting, cathepsi

51 Fluorogenic substrates based on 4-methylumbelliferone (4

52 tral enzyme of this network, by developing a fluorogenic substrate-based method for time- and space-r

53 like activity as assessed by the cleavage of fluorogenic substrate benzyloxycarbonyl-DEVD-7-amido-4-(

54 .02 s-1 for the enzymatic hydrolysis of this fluorogenic substrate by FIV PR under the conditions of

55 ress curves of the hydrolysis of a sensitive fluorogenic substrate by FXIa in the presence of PN-2 to

56 Upon cleavage of the fluorogenic substrate by SPase I, the fluorescent intens

57 The strategy utilized in the design of this fluorogenic substrate can be applied in future endeavors

58 ps for performing enzymatic assays for which fluorogenic substrates cannot easily be designed.

59 We synthesized and optimized a fluorogenic substrate capable of reporting on granzyme B

60 Fusion was detected by cleavage of the fluorogenic substrate CCF2 by beta-lactamase-Vpr incorpo

61 ate cleavage pattern of human AMCase against fluorogenic substrates, chitobiose-4-methylumbelliferyl

62 , while matriptase activity was monitored by fluorogenic substrate cleavage.

63 Fluorogenic substrates composed of a fluorophore possess

64 Turnover was observed via fluorogenic substrate conversion and fluorescence micros

65 The fluorogenic substrates detect activation at much lower c

66 forms by immunoblots and by cleavage of the fluorogenic substrate DEVD-AFC.

67 tivity, as measured in cell lysates with the fluorogenic substrate DEVD-AMC, was elevated almost imme

68 In this work, we report readily synthesized fluorogenic substrates enabling enzyme-economical evalua

69 this paper, we describe the development of a fluorogenic substrate for 17beta-hydroxysteroid-dehydrog

70 h ALDH activity, detected using Aldefluor, a fluorogenic substrate for ALDH.

71 ge of DEVD-aminomethylcoumarin (DEVD-AMC), a fluorogenic substrate for caspases 2, 3, and 7; in contr

72 A novel fluorogenic substrate for continuous feline immunodefici

73 using pH-sensitive fluorescent probes and a fluorogenic substrate for cysteine proteases showed that

74 no, 4-methyl coumarin amide (AAMCA), a novel fluorogenic substrate for FAAH, was designed and synthes

75 A fluorogenic substrate for HIV-1 protease was designed an

76 Using a fluorogenic substrate for phospholipase A(2) (PLA(2)), w

77 plication of a novel type of chromogenic and fluorogenic substrate for protease detection is describe

78 We have developed a new fluorogenic substrate for the alpha-glucosidase enzyme a

79 cein di-beta-d-galactopyranoside, which is a fluorogenic substrate for the intracellular enzyme beta-

80 A new fluorogenic substrate for the specific detection of orga

81 the identification and characterization of a fluorogenic substrate for tumor necrosis factor-alpha co

82 re assayed with a novel, membrane-impermeant fluorogenic substrate for vp165, we found that insulin s

83 ethylrhodamine) have been used previously as fluorogenic substrates for a number of DNA modifying enz

84 r enzyme fluorescence (REF) that uses custom fluorogenic substrates for bacterial enzymes allows rapi

85 k through development of near-infrared (NIR) fluorogenic substrates for beta-lactamase, an enzyme exp

86 Analysis with fluorogenic substrates for caspase activity confirmed th

87 Here we report the synthesis of fluorogenic substrates for glycosidases based on a sulfo

88 bes a design of cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase expres

89 A series of 54 fluorogenic substrates have been synthesized and evaluat

90 plarily demonstrated for the cleavage of the fluorogenic substrate hydrodabcyl-Ser-Phe-EDANS by the p

91 due to the complication involving the use of fluorogenic substrates in in vitro assays.

92 ating and neighboring cells using orthogonal fluorogenic substrates in various mixed cell systems.

93 reaction to convert a novel, cell-permeable fluorogenic substrate into fluorescein within living cel

94 Therefore, this new fluorogenic substrate is a useful tool for the alpha-glu

95 ed lipid vesicles, and their reaction with a fluorogenic substrate is probed.

96 The rate of hydrolysis of the fluorogenic substrates is proportional to enzyme concent

97 the widely used Amplex Red, as well as other fluorogenic substrates, is often overlooked.

98 In this paper, we describe a novel fluorogenic substrate, KLTFGTVK(Abz)PVQAIAGY(NO2)EWL, in

99 tered the interaction of the enzyme with the fluorogenic substrate (Leu-Arg-Gly-Gly-NH)2-rhodamine.

100 agents led to the cleavage of the caspase-4 fluorogenic substrate, LEVD-7-amino-4-trifluoromethylcou

101 Using combinatorial fluorogenic substrate libraries, the P1-P4 substrate spe

102 ty was assayed by monitoring cleavage of the fluorogenic substrate Mca-RPPGFSAFK(Dnp)-OH, a derivativ

103 HRP first oxidizes fluorogenic substrate molecules like Amplex Red to radic

104 ochemistry, immunoblots, and cleavage of the fluorogenic substrate N-benzyloxycarbonyl-Asp-Glu-Val-As

105 ghly sensitive, selective, and tight binding fluorogenic substrate of a copper amine oxidase that is

106 We report a novel fluorogenic substrate of bovine plasma amine oxidase (BP

107 The 14-amino-acid fluorogenic substrate of sequence RALTK(Abz) VQ approxim

108 Tx was detected, as shown by the cleavage of fluorogenic substrates of the proteasome.

109 ity of rat nardilysin was investigated using fluorogenic substrates of the type 2-aminobenzoyl-GGX(1)

110 culture by introducing a combination of two fluorogenic substrates or a fluorogenic and a luminogeni

111 ty was comparable with results obtained with fluorogenic substrates or fluorochrome-labeled inhibitor

112 The chromogenic and fluorogenic substrates, p-nitrophenyl-alpha-D-glucopyran

113 The novel chromogenic and fluorogenic substrates, p-nitrophenyl-beta-D-glucopyrano

114 (Danio rerio) CYP1 isoforms with a series of fluorogenic substrate probes was determined by the rate

115 6 kDa proteolytic fragments with a synthetic fluorogenic substrate produced hyperbolic substrate vers

116 le Michael or Diels-Alder reactions of these fluorogenic substrates provide products of significantly

117 e in fluorescence on caspase cleavage of the fluorogenic substrates ranged from 40 to 83 depending on

118 These fluorogenic substrates release highly fluorescent BODIPY

119 veloped a high-throughput assay based on the fluorogenic substrate reporter WH-15.

120 Ti(2) and i(2)ANDNOTi(1) gates that cleave a fluorogenic substrate, reporting through an increase in

121 cs an i(1)ANDi(2) gate and cleaves the other fluorogenic substrate, reporting through an increase in

122 trates are required in costly amounts, while fluorogenic substrates require an additional apparatus (

123 ts, containing varying concentrations of the fluorogenic substrate resorufin beta-d-galactopyranoside

124 el in human plasma, employing ecarin and the fluorogenic substrate, resulted in improvement of the de

125 ave combined the GzB substrate with a second fluorogenic substrate selective for caspase 3 to allow b

126 the TF.factor VIIa (FVIIa) complex toward a fluorogenic substrate showed that the catalytic efficien

127 s was developed using the thrombin-specific, fluorogenic substrate SN-59 (100 muM).

128 y inhibit serine protease activity against a fluorogenic substrate specific for TLSPs.

129 The preparation was active against fluorogenic substrates specific for cathepsin D and E an

130 s by fluorescence polarization assay against fluorogenic substrates specific for MMPs or TLSPs.

131 Calpain activity was assayed using the fluorogenic substrate Suc-Leu-Leu-Val-Tyr-7-amino-4-meth

132 Data from both sets of fluorogenic substrates supported the contribution of a P

133 Fluorogenic substrate tests provided a sensitive and sim

134 Our objective was to create a novel fluorogenic substrate that could be used for real-time d

135 ed components are mixed with an enzyme and a fluorogenic substrate that permits to follow the enzymat

136 compounds should provide a diverse range of fluorogenic substrates that may be used to rapidly scree

137 The fluorogenic substrates that we describe and their sequen

138 We show here, using fluorogenic substrates, that FZ treatment leads to the i

139 When loaded with a cell-permeable fluorogenic substrate, the cell library simultaneously r

140 n droplet microfluidic screening systems use fluorogenic substrates to measure enzymatic activities w

141 Mildly permeabilized cells were exposed to fluorogenic substrates to monitor the activity of intrac

142 low micromolar range) in competition with a fluorogenic substrate toward a recombinant form of the t

143 dish peroxidase, patterned downstream, where fluorogenic substrate turnover was recorded.

144 ded MBL)) and the identification of suitable fluorogenic substrates (umbelliferone-derived cephalospo

145 A validated MT1-MMP fluorogenic substrate was infused through the microdialy

146 hancement of precision and sensitivity using fluorogenic substrates was demonstrated in a direct-bind

147 The efficiency of the fluorogenic substrates was exemplified by inhibitor scre

148 The fluorogenic substrates were compared to chromogenic subs

149 Two fluorogenic substrates were prepared, one using a covale

150 Hypersensitive fluorogenic substrates were used to determine steady-sta

151 or the variants were determined with a novel fluorogenic substrate, which was designed to match the n

152 ANG cleaves a dinucleotide version of the fluorogenic substrates with a k(cat)/K(M) value of 61 M(

153 analysis of a series of otherwise identical fluorogenic substrates with Lys at P1 and different resi

154 Fluorogenic substrates with P1, P2, and P4 basic amino a

155 ge by purified mutant enzymes of a series of fluorogenic substrates with systematic changes in P(4) a

156 ently monitored with peptidic chromogenic or fluorogenic substrates, with certain peptide sequences i

157 ot protect monocytes from apoptosis, and the fluorogenic substrate YVAD-AFC failed to show an increas

158 ssue section directly in a cuvette using the fluorogenic substrate Z-arg-arg-AMC.

159 latin zymography in SDS-PAGE, and by in situ fluorogenic substrate zymography in RPE/choroid sections