1 cell lines that survive in the presence of 5-fluoroorotic acid.

2 er gene and yields sensitivity to the drug 5-fluoroorotic acid.

3  through cellular sensitivity to the drug, 5-fluoroorotic acid.

4  of ura3 using a uracil dropout medium and 5-fluoroorotic acid.

5 tandard genetic methods involving the URA3/5-fluoroorotic acid (5-FOA) counter-selection have shown a

6 media that either lacks uracil or contains 5-fluoroorotic acid (5-FOA), an unusual dual phenotype tha

7  H. salinarum was shown to be sensitive to 5-fluoroorotic acid (5-FOA), which can select for mutation

8 ome VII-L that can be counterselected with 5-fluoroorotic acid (5-FOA).

9 ed by uracil prototrophy using the reagent 5-fluoroorotic acid (5-FOA).

10              A third counterselection with 5-fluoroorotic acid (5FOA) against yeast clones retaining

11     Using the toxicity of the DctA substrate fluoroorotic acid as a selective agent in an iterated se

12                           Thus, plating on 5-fluoroorotic acid causes the loss of thiamine synthesis

13     Following selection for Ura- clones on 5-fluoroorotic acid containing medium, the following types

14  are reported: 1-(beta-d-erythrofuranosyl)-5-fluoroorotic acid (FEO) and 5'-deoxy-5-fluoroorotidine (

15 n results in cell death in the presence of 5-fluoroorotic acid (inducible reverse two-hybrid assay).

16 URA3 reporters and negative selection with 5-fluoroorotic acid, it is shown that Ty1 genes can underg

17 ed by dual selection with hygromycin B and 5-fluoroorotic acid, or by screening hygromycin B-resistan

18     In contrast to wild-type cell lines, the fluoroorotic acid-selected cell lines did not respond to

19 d formation, and spontaneous resistance to 5-fluoroorotic acid, which is a selective agent for URA5 g

20 imidine biosynthesis and were resistant to 5-fluoroorotic acid, which is converted to a toxic product