ライフサイエンスコーパス: fluorophore

1 , TH peptide), and imaging probes (i.e., Cy5 fluorophore).

2 chment point for a nitrobenzoxadiazole (NBD) fluorophore.

3 n releasing the cleaved product containing a fluorophore.

4 on binding of a cell-permeable and non-toxic fluorophore.

5 the photochrome and a co-encapsulated BODIPY fluorophore.

6 tility to a light-controllable, pH-sensitive fluorophore.

7 ing a single DNA probe labeled with a single fluorophore.

8 othiadiazole (BTD)-based rationally designed fluorophore.

9 rs lessons for proteins without this visible fluorophore.

10 istribution of blinking events from a single fluorophore.

11 yoverdine to pyoverdine, which completes the fluorophore.

12 emission signatures of organic and inorganic fluorophores.

13 )nanodiamonds in the presence of interfering fluorophores.

14 ction between metallic substrates and single fluorophores.

15 ns to quantify the lifetimes of contributing fluorophores.

16 om binding of either endogenous or exogenous fluorophores.

17 cquisition speed, and compatibility with all fluorophores.

18 d functioning of a highly important class of fluorophores.

19 0-silaxanthone, and 9-imino-10-germaxanthone fluorophores.

20 limited by the need for spectrally distinct fluorophores.

21 f an ensemble of fluorophores down to single fluorophores.

22 ce resonance energy transfer between the two fluorophores.

23 benzocyclooctyne- and bicyclononyne-modified fluorophores.

24 blinking is observed in nearly all nanoscale fluorophores.

25 ater photon yield than obtained using common fluorophores.

26 ules were assessed with parameter-indicating fluorophores.

27 us sensors to measure the ratios of absorbed fluorophores.

28 rophores were combined with six conventional fluorophores, 10-color probe detection in a single PCR t

29 itoring in the form of a diazaoxatriangulene fluorophore, 1a (hexadecyl side chain).

30 interfacial pH change is revealed using the fluorophore 8-hydoxypyrene-1,3,6-trisulfonic acid.

31 , 4,7'-o-PBBV, and pBoB, each coupled to the fluorophore 8-hydroxypyrene, 1,3,6-trisulfonic acid tris

32 tructures labeled with a benzofuranyluridine fluorophore act as analytes.

33 By tagging protein or RNA subunits with fluorophores, activating them, and subsequently observin

34 zation allows preparation of conjugates with fluorophore, affinity handle, and pharmacological agents

35 The effect of fluorophore aggregation on its RRS, UV-vis, R2S2, and SS

36 wt % 1,4-bis(2-methylstyryl)benzene singlet fluorophore and 0.28 wt % Ir(3+), respectively.

37 amino-4-carbamoylmethylcoumarin (ACC) as the fluorophore and 2,4-dinitrophenyl-lysine (Lys(DNP)) as t

38 s for Sau96I and SacII, and is attached to a fluorophore and a quencher.

39 e probe, containing a conformation-sensitive fluorophore and an anomalous X-ray diffraction label (5-

40 ies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify

41 ission intermittency in a quantum mechanical fluorophore and identifiable chemical reactions that ult

42 ly breaks down, leading to separation of the fluorophore and quencher.

43 Urinary fluorophore and sugar ratios reflect gut injury in an in

44 photoactivation or photoswitching of single fluorophores and position determination (single-molecule

45 unity to construct environmentally sensitive fluorophores and probes.

46 Both fluorophores and the antibiotic levofloxacin are attache

47 With rotationally confined lipophilic fluorophores and the polarized incident fluorescence exc

48 r analysis (PARAFAC) extracted the potential fluorophores and their values in the PDO vinegars.

49 principles for fine-tuning the properties of fluorophores and to develop a palette of new fluorescent

50 d photobleaching of several types of organic fluorophores and/or the interference generated by biolog

51 clizations and oxidations that result in the fluorophore, and a strictly conserved fluorophore-bound

52 , including amino acids, a monosaccharide, a fluorophore, and an analogue of the cytotoxic drug duoca

53 acid) (PLGA) NPs were loaded with BODIPY, a fluorophore, and percutaneously administered into the pe

54 ocalization microscope to image a particular fluorophore, and suggests that development of probes for

55 ) labeling to couple surface DAT directly to fluorophore, and tracked DAT's postendocytic itinerary i

56 rvation of time-resolved fluorescence, Alexa-fluorophores, and horse radish peroxidase-based bead ass

57 Diol sensors are often based on fluorophore-appended boronic acids, but these severely l

58 e free end of RNA, with the long axis of the fluorophores approximately parallel to that of the termi

59 ylboronic acid-based receptors with appended fluorophores are commonly used as molecular sensors for

60 nce can be recorded at those positions where fluorophores are found within a subresolution neighborho

61 Fluorophores are important but optically complicated pho

62 ls for immunofluorescence with small organic fluorophores are lacking.

63 scattering is less efficient, and endogenous fluorophores are less likely to absorb.

64 By adding in glutathione, fluorophores are made to blink, enabling super-resolutio

65 by trapping a globular protein or individual fluorophores are not.

66 Functional fluorophores are powerful tools for such studies.

67 The fluorophores are stacked on the terminal basepair such t

68 es in the microenvironment of the respective fluorophores, as evidenced by changes in the voltage dep

69 le reprogramming was most efficient with the fluorophore at the second position.

70 ence fluctuations of individual pH-sensitive fluorophores at the membrane surface by fluorescence cor

71 Moreover, fluorophores attached to haloarenes are noteworthy, but

72 ndicators require direct modification of the fluorophore backbone through complex synthetic considera

73 cond near-infrared window (NIR-II) molecular fluorophores based on a shielding unit-donor-acceptor-do

74 We employed a novel combination of fluorophore-based confocal laser scanning microscopic im

75 One-fluorophore-based sensors have four Ca(2+)-binding sites

76 escently labelled with sufficient numbers of fluorophores because the lysine, arginine and cysteine r

77 n of miRNA-mRNA interactions combined with a fluorophore-binding sequence 'Spinach', a GFP-like RNA a

78 ments into spatial proximity, which formed a fluorophore-binding site in situ and turned on fluoresce

79 It minimizes fluorophore bleaching as well as phototoxic effects and

80 molecule localization micro-scopy studies of fluorophore blinking offer a promising route to probe ol

81 allows us to determine molecule counts from fluorophore blinking statistics.

82 due 114 with the environmentally insensitive fluorophore BODIPY or the pH-sensitive dye pHrodo red.

83 in the fluorophore, and a strictly conserved fluorophore-bound glutamic acid residue is converted to

84 ation of the signal amplitude is observed in fluorophores but not in endogenous chromophores.

85 n its ORF counterparts for all the six model fluorophores, but more than 6 orders of magnitude larger

86 the design, preparation, and fine-tuning of fluorophores by means of multicomponent reactions, C-H a

87 surfaces alter the fluorescence intensity of fluorophores by modulating both the excitation and light

88 Enterally administered fluorophores can identify intestinal injury in a rat mod

89 However, the stochastic blinking of single fluorophores can introduce large uncertainties into the

90 pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that

91 iting the generalization of designs to other fluorophore classes.

92 with alpha-boryl acetals containing a latent fluorophore clearly demonstrate that cargo can be releas

93 arget recognition, fluorescence imaging, and fluorophore cleavage, this approach has the potential to

94 h', a GFP-like RNA aptamer for which the RNA-fluorophore complex exhibits strong and consistent fluor

95 A simple three component array of host-fluorophore complexes is capable of sensitive and select

96 ded fluorophore, we exploit a series of host-fluorophore complexes that are affected by the presence

97 the excited state and is independent of the fluorophore concentration.

98 ustrated by generating a functional antibody-fluorophore conjugate.

99 NE to analyze the simultaneous binding of 15 fluorophore-conjugated Abs and one cell viability probe

100 o analyze individual organelles labeled with fluorophore-conjugated antibodies, and the first applica

101 ficiently labeled with phosphine-derivatized fluorophore-conjugated bovine serum albumin.

102 Subsequent bioorthogonal reactions with the fluorophore-conjugated DpTz reagents afforded the fluore

103 s, functionalized with a near infrared (NIR) fluorophore-conjugated immunoassay to Carbohydrate Antig

104 brane dynamics that was enabled by trehalose-fluorophore conjugates capable of labeling trehalose gly

105 e as bright as two commercial anti-EpCAM red fluorophore conjugates, APC and AlexaFluor(R)647.

106 As a proof-of-principle, fluorophores containing a borinate (RF620 ) or silanedio

107 d/or require specialized equipment and other fluorophore-containing reagents.

108 r fluorescence imaging and data storage, the fluorophores coupled to the antibodies are efficiently c

109 as well as efficient maleimide chemistry for fluorophore coupling.

110 Their site-specific labeling with multiple fluorophores creates bright imaging reagents for confoca

111 c-gold nanoparticles and the long wavelength fluorophore cresyl violet, has been used for the determi

112 The indodicarbocyanine fluorophores Cy3 and Cy5 are extensively used as donor-a

113 ate recovery dynamics of the single-molecule fluorophore Cy3B in the presence of four different photo

114 cles (CLIO) derivatized with a near-infrared fluorophore (CyAm7) 24 hours before near-infrared fluore

115 We found that the expression of fluorophores decreased as it was moved towards the end o

116 ting of activity-based probes with different fluorophore-detecting groups with minimal wavelength ove

117 However, the RRS features of fluorophores differ drastically.

118 based on the intensity changes of a tethered fluorophore, diffusing in the exponentially decaying eva

119 ctin using phalloidin conjugated with common fluorophores display different levels of fluorescence on

120 Both these fluorophores display far-red to near-infrared excitation

121 te the fluorescence signal of an appropriate fluorophore donor in a distance-dependent manner.

122 f molecules from the level of an ensemble of fluorophores down to single fluorophores.

123 NIR-II fluorophores draw from a broad spectrum of materials spa

124 that minimizes both the overlap of paper and fluorophore emission spectra and the fluorescence intens

125 interconversion, and the fraction of active fluorophores enter as two or three constants.

126 elds were quantified for the model molecular fluorophore Eosin Y and fluorescent Qdots CdSe and CdSe/

127 the proper choice of GNR size to overlap the fluorophore excitation and emission wavelengths greater

128 elates with the spectral overlap between the fluorophore excitation/emission and the plasmonic resona

129 hods, including sensorFRET, fail at specific fluorophore-excitation wavelength combinations.

130 s two-photon imaging and real-time analysis, fluorophore-expressing neurons of multiple types in the

131 (PRV) strains in which viral propagation and fluorophore expression are activated only after exposure

132 power to be optimized for varying levels of fluorophore expression.

133 the reductive fluorescence quenching of five fluorophores ("F") by six quenchers ("Q"; electron donor

134 arrays allow for development of a family of fluorophores featuring a common excitation band at 500 n

135 A new class of push-pull fluorophores featuring the pentafluorosulfanyl (SF5) gro

136 Far-red cyanine fluorophores find extensive use in modern microscopy des

137 ackbone for charge variations, near-infrared fluorophores for bioimaging, and beta-cyclodextrins for

138 self-propagating cycle that generates three fluorophores for each single fluoride released.

139 Our study supports the idea of using ACQ fluorophores for imaging and characterizing the fate of

140 wire-based voltage sensors with 2P-optimized fluorophores for imaging voltage in intact brain tissue.

141 covalent cross-linking and for reaction with fluorophores for in vitro and in vivo imaging.

142 and can be carried out with photoswitchable fluorophores for multicomponent discrimination, to deter

143 nresolved, possibly representing reoccurring fluorophores forming noncovalently bound assemblies of v

144 mers and monomers of gold nanoparticles) and fluorophore-functionalized colloids (fluorescent polymer

145 The fluorophores generated smooth curvilinear ratio trajecto

146 ode based on the bulk aggregate formation of fluorophore has never been reported, and this may open a

147 ol of the fluorescence properties with Au NP/fluorophore hybrids loaded on the rotaxane scaffold, are

148 In multicellular organisms, single-fluorophore imaging is obstructed by high background.

149 beta-barrel structure that encapsulates the fluorophore in the protein.

150 lial cells), and without interference of the fluorophore in uptake processes.

151 and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast a

152 h preliminary data on detection accuracy for fluorophores in a mixture.

153 ergy transfer (FRET) processes by fixing the fluorophores in a rotaxane and (iv) establishing the pri

154 optimized by tuning the density of activated fluorophores in each time frame.

155 l relationships between the positions of the fluorophores in space and time.

156 Bright fluorophores in the near-infrared and shortwave infrared

157 orporating target-sensitive and target-inert fluorophores into a single conjugated polymer to avoid l

158 We internalized tRNA labeled with organic fluorophores into live bacteria, applied single-molecule

159 as the shielding unit, the best performance fluorophores IR-FE and IR-FEP exhibit an emission quantu

160 (SuCESsFul) biosensors, in which a reporting fluorophore is conjugated to a binding scaffold, can, in

161 y engineered mouse model was used in which a fluorophore is expressed under the TRPV1 promoter.

162 y synthesized peptide ferribactin, and their fluorophore is generated by periplasmic oxidation and cy

163 The formation of the fluorophore is known to be driven by the periplasmic tyr

164 e intrinsic fluorescence of a small-molecule fluorophore is masked by esterification until entry into

165 e that when FRET occurs, energy from a donor fluorophore is transferred to an acceptor fluorophore on

166 Knowledge of the positions of the fluorophores is critical to the interpretation of fluore

167 rter is required or desirable, the choice of fluorophores is rather limited.

168 The capability of near-infrared fluorophore-labeled and (99m)Tc- and (111)In-labeled der

169 of membrane-bound immunoglobulin G (IgG), a fluorophore-labeled anti-mouse IgG antibody (anti-IgG-Al

170 ion to select candidate paper substrates for fluorophore-labeled assays.

171 The fluorophore-labeled C2Am derivative showed predominantly

172 Fluorophore-labeled cytochrome c oxidase displayed a sim

173 Central or peripheral administration of a fluorophore-labeled GLP-1R agonist, exendin-4, localizes

174 knowledge of the total surface densities of fluorophore-labeled membrane proteins is essential for c

175 ells, tissues or embryos through analysis of fluorophore-labeled PCR amplicons covering the nuclease

176 HRP), which is subsequently incubated with a fluorophore-labeled polyclonal anti-peroxidase antibody

177 ard primers, three reverse primers, and four fluorophore-labeled probes in a single-reaction format.

178 Subsequent D-AB3 conjugation to a fluorophore-labeled semicarbazide allows robust detectio

179 nt enzymatic displacement and quenching of a fluorophore-labeled strand, which happens only if both S

180 esolution of two photoactivatable/switchable fluorophore-labeled structures in the same frozen sample

181 article (AuNP) require that the sequences be fluorophore-labeled, which may affect the DNA surface co

182 ons, field dependence in the imaging system, fluorophore labeling density, and registration between d

183 the intrinsic orientational mobility of the fluorophore labels and the real structural disorder of t

184 termediate that cyclizes to release a masked fluorophore leading to fluorescence enhancement.

185 These ssDNA arrays can be used to detect fluorophore-linked DNA oligonucleotides in solution, and

186 in Western blotting, in both peroxidase- and fluorophore-linked form.

187 After spraying and drying, fluorophore-loaded filomicelles that were originally 10

188 =24 nm was detected with PLM, and individual fluorophore localization precision was 13 +/- 5 nm.

189 FRET is generated by energy transfer between fluorophores located in separate oligomers in the two-di

190 rescence, ECL emission is only detected from fluorophores located in the immediate vicinity of the el

191 Based on recent studies, fluorophores-mainly aromatic amino acids (AAAs) in prote

192 cloned from Daphnia pulex, were fused to the fluorophore, mAmetrine and the photon generator, Rluc2,

193 tro fertilization, where contrast agents and fluorophores may impact the viability of the embryo.

194 nts indicated that the aqueous nature of the fluorophore microenvironment depended on the membrane po

195 The main important classes of fluorophore molecules in red wine were identified by Fro

196 gonist binding pocket) was labelled with the fluorophore MTS-TAMRA and changes in fluorescence on ago

197 The lipid soluble fluorophore Nile Red (9-diethylamino-5-benzo[alpha]pheno

198 nsity state of the directly excited acceptor fluorophore on a DNA tether are due to a change of its a

199 Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellabl

200 or fluorophore is transferred to an acceptor fluorophore only when certain conditions are met.

201 to site-specifically incorporate a chemical fluorophore onto a single subunit of heterotrimeric RPA.

202 Existing studies on fluorophore optical properties have been focused almost

203 ger that is capable of masking a phenol on a fluorophore or any other potential chemical scaffold for

204 Secondary dopants comprising singlet fluorophores or iridium organometallic compounds provide

205 difications in a site-specific manner (e.g., fluorophores or photo-labile groups).

206 s enables the experimental separation of the fluorophore ORF and photon scattering features in the fl

207 ping new analytical methods that exploit the fluorophore ORF and photon scattering properties.

208 A mathematical model for calculating fluorophore ORF and scattering cross sections has been d

209 Another finding is that the fluorophore ORF has a depolarization close to 1, while i

210 hese include dependence on both distance and fluorophore orientation.

211 derivatives of Taxol linked to the drug-like fluorophore Pacific Blue (PB).

212 indicated negligible interaction between the fluorophore part of camptothecin and the hydrogel, espec

213 e heart influenced BODIPY distribution, with fluorophore penetrating more readily into atria than ven

214 brightness standards, demonstrating that 80 fluorophores per diffraction-limited spot can be readily

215 ting them, and subsequently observing as the fluorophores photobleach, one obtains information on the

216 on of fluorescent signals in the presence of fluorophore photobleaching in a solid surface bioassay.

217 rescence (SSF) with scant information on the fluorophore photon scattering and on-resonance fluoresce

218 ires high-density labeling and extraordinary fluorophore photostability.

219 LIM) can measure and discriminate endogenous fluorophores present in biological samples.

220 al wall were consistent with the emission of fluorophores present in healthy arterial wall (collagen,

221 igated the effect of external buffers on the fluorophore proton exchange rates at the ND membrane-wat

222 Each cleavage event separates fluorophore quencher pairs resulting in an increase in f

223 to DNA strands conjugated to a near-infrared fluorophore/quencher pair (IRDye 800CW/IRDye QC-1) with

224 ents were performed with a voltage-dependent fluorophore/quencher pair.

225 Fluorophore ratios are measureable through the skin, obv

226 Fluorophore ratios measured through the skin distinguish

227 t arises from a large population of untagged fluorophores; rendering these labels potentially ineffec

228 re ORF and photon scattering features in the fluorophore resonance synchronous spectra.

229 The fluorophore responsible for the MM1 marker has been iden

230 photon transfer from the photon donor to the fluorophore resulting in fluorescence emission.

231 ite to changes in the fluorescence of a core fluorophore scaffold.

232 These dyes are expected to be useful fluorophore scaffolds in the development of new NIR prob

233 y of novel functional groups inserted within fluorophore scaffolds.

234 The fluorophore scattering cross section is approximately 10

235 Our FISH protocol with single-fluorophore sensitivity significantly reduces cost and t

236 which works as an environmentally sensitive fluorophore, showing high fluorescence in lipophilic con

237 super-resolution microscopy, we examined how fluorophore size and photostability affected AMPAR traff

238 All fluorophore spectra comprise a well-defined Gaussian pea

239 erimental approach for quantification of the fluorophore SSF, ORF, and scattering depolarization and

240 cleic acids via the 5' phosphate group these fluorophores stack onto the ends of the molecule.

241 xFRET, pFRET), as it does not require single fluorophore standards to be measured with every experime

242 Thus, as fluorophores stochastically photobleach, noise propertie

243 ttering of long-wavelength photons and large fluorophore Stokes shifts.

244 f four-membered azetidine rings into classic fluorophore structures elicits substantial increases in

245 Scaffold conjugated to environment-sensitive fluorophore (SuCESsFul) biosensors, in which a reporting

246 To investigate its biological activity, fluorophores such as Oregon Green have been linked to th

247 Small-molecule fluorophores, such as fluorescein and rhodamine derivati

248 tern-Volmer quenching of active site-labeled fluorophore suggested that the allosteric regulators ind

249 , as the uptake of systemically administered fluorophore-tagged exendin-4 was blocked by central pret

250 Sugar binding and transport by the fluorophore-tagged mutants were blocked, but Na(+) bindi

251 Moreover, mutant cells containing fluorophore-tagged receptors exhibited greater basal ani

252 es and in liposomes, and contain red-shifted fluorophores that are suitable for high-throughput scree

253 voted to the development of amino acid-based fluorophores that do not require a specific environment

254 surements probe the distance between Cy3/Cy5 fluorophores that label the ends of a short (15-nt) segm

255 ing single molecules with multiple, blinking fluorophores, the accuracy of the technique can be enhan

256 In fluorophores, the excited state lifetime can be modulate

257 , we demonstrate that in complex mixtures of fluorophores, the relative concentration information is

258 In this method, antibodies tethered to fluorophores through a novel azide-based cleavable linke

259 The genomic tagging of PALM fluorophores through CRISPR-Cas9 offers an excellent opp

260 lting in the highest quantum yield molecular fluorophore thus far.

261 The chemical confinement of a pH sensitive fluorophore to a thin-reaction layer adjacent to an elec

262 exa Fluor 488, which covalently attaches the fluorophore to the alkyne moiety in the compounds.

263 Without the conjugation of the fluorophore to the CDs, the particle aggregation-induced

264 Tethering of the released fluorophore to the cellulose surface prevents signal deg

265 Since the stoichiometry of the fluorophore to the dendrimer is 1:1, we were able to dir

266 , are commonly included in the structures of fluorophores to impart hindered intramolecular rotations

267 ple probes labelled with spectrally distinct fluorophores to multiple sub-regions of single mRNAs, an

268 This confirms the tendency of both fluorophores to stack on the free end of RNA, with the l

269 The fluorescence responses of 4 fluorophores upon addition of commercial milk samples in

270 t can be used to recover the location of the fluorophore using difference imaging.

271 zation and anisotropy using a combination of fluorophore UV-vis, fluorescence emission, and resonance

272 We used IRDye800CW as the fluorophore, validated performance characteristics for s

273 The ECL fluorophore was linked to an antibody and enabled to loc

274 absorbance and fluorescence spectrum of the fluorophore was not affected by pH and temperature.

275 The protonation rate of the fluorophores was approximately 100-fold higher when loca

276 e release and activation of a small molecule fluorophore, we constructed AND and OR logic gates that

277 toring the change in emission of an appended fluorophore, we exploit a series of host-fluorophore com

278 his was observed when the donor and acceptor fluorophores were attached in AR3 (residue 166) and AR6

279 When these four Stokes-shift fluorophores were combined with six conventional fluorop

280 Four new large-Stokes-shift fluorophores were developed.

281 eral method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing

282 such as high duplex stability and a built-in fluorophore, which would augment its application potenti

283 articles may be identified by the integrated fluorophores, which are "turned off" when the particles

284 nanofibres are easily fabricated to contain fluorophores, which make the dielectric nanofibre and mu

285 etes the palette of known squaraine rotaxane fluorophores whose emission profiles span the color rang

286 CDs as fluorophore with average size 3-4nm were prepared by hyd

287 Also, interaction of the fluorophore with the binding protein it is conjugated to

288 Caging a fluorophore with this ion-sensitive moiety yields an "io

289 d in cellulo, yielding a bright, photostable fluorophore with utility in biomolecular imaging.

290 evived as a tool to impart synthetic organic fluorophores with 'self-healing' properties.

291 gement of fluorescently labeled drugs, using fluorophores with a fluorescence lifetime larger than th

292 microscopy (SMLM) allows the localisation of fluorophores with a precision of 10-30 nm, revealing the

293 Using fluorophores with different maturation kinetics to label

294 Fluorophores with dynamic or controllable fluorescence e

295 e microscopy requires the design of enhanced fluorophores with finely tuned properties.

296 Development of fluorophores with optimized photoswitching will consider

297 he primer extension products was achieved by fluorophores with reactive counterparts and analyzed by

298 suggest a novel approach that uses different fluorophores with the same emission maximum.

299 ng solubilized by detergent and quenching of fluorophores within the vesicles, which makes them easy

300 non-photobleaching and can be used alongside fluorophores without interference.

301 gst these, probe 3 containing a zwitterionic fluorophore, ZW800-1, combined a favorable affinity prof