ライフサイエンスコーパス: forward mutation

1 elevated levels of nitrogen mustard-induced forward mutation.

2 igh selection coefficient (s > 0.1) for each forward mutation.

3 sequences and raises the rate of spontaneous forward mutation.

4 -52, rth1Delta/rad27Delta) mutations in both forward mutation and repeat-tract instability assays.

5 c heat stress caused elevated frequencies of forward mutations and interchromosomal DNA recombination

6 e of base substitution mutations by the CAN1 forward mutation assay compared to the rate for the wild

7 Here, we describe a pGAL-CAN1 forward mutation assay for studying transcription-associ

8 Use of an Escherichia coli lac forward mutation assay has shown that this procedure red

9 ion of the mutational spectra generated by a forward mutation assay revealed that errors in DNA repai

10 Here, we use a forward mutation assay to systematically examine the acc

11 We have developed an in vitro DNA polymerase forward mutation assay using damaged DNA templates that

12 The HSV-tk forward mutation assay was utilized to determine the cor

13 Most of the R2 base substitutions in the forward mutation assay were caused by the misincorporati

14 In a lacZalpha forward mutation assay, A661E and T664R yielded mutation

15 We adapted a forward mutation assay, originally developed for lambda

16 sphoribosyltransferase (APRT(+)) --> APRT(-) forward mutation assay, we found that while Cr(VI) alone

17 By a yeast forward mutation assay, we show that strengthening RNA f

18 ower fidelity as demonstrated by a PCR-based forward mutation assay.

19 e-associated 3'-5' exonucleases using a lacZ forward mutation assay.

20 spectively, as measured by the M13 lacZalpha forward mutation assay.

21 d by using single-molecule PCR or an in vivo forward mutation assay.

22 ase in mutation frequency in the M13mp2 lacZ forward mutation assay.

23 rpes simplex virus-thymidine kinase (HSV-tk) forward mutation assay.

24 reduced mutation rate in an M13mp2 lacZalpha forward mutation assay.

25 n DNA copying fidelity in an M13 phage-based forward mutation assay.

26 merase fidelity of HIV-1 RT via an M13-based forward mutation assay.

27 (about 20-fold) as determined by a rifampin forward mutation assay.

28 polymerases were compared using a PCR-based forward mutation assay.

29 and measured its fidelity using a LacZ-based forward mutation assay.

30 In the experiments reported here, the SUP4-o forward-mutation assay was used to monitor GC --> TA mut

31 quired for suppressing mutations in the CAN1 forward-mutation assay.

32 Forward mutation assays reveal a 6-fold reduction in the

33 irement for this regulation, we employed two forward mutation assays to characterize PrimPol's replic

34 However, forward mutation assays using a lacZ alpha reporter gene

35 fidelity in both misincorporation assays and forward mutation assays, but display a substantially hig

36 and replication error rates in M13 lacZalpha forward mutation assays, similar to HIV-1 RT.

37 s and hypoxanthine phosphoribosyltransferase forward mutation assays.

38 hermophilic archaea, a quantitative assay of forward mutation at extremely high temperature was devel

39 The measured rates of forward mutation at the pyrE and pyrF loci in S. acidoca

40 pH 3.5 and 75 degrees C based on the rate of forward mutation at the pyrE gene and the nucleotide cha

41 , thus comprising a significant component of forward mutations at this locus.

42 bstitutions detected, a majority represented forward mutations away from the consensus sequence.

43 Because of the large size of the LYS2 locus, forward mutations first were mapped to specific LYS2 sub

44 Forward mutation frequencies and rates were measured at

45 ic conditions showed a powerful induction of forward mutation frequencies compared to wild-type cells

46 Measurement of forward mutation frequencies in msh2Delta rfc1:Tn3 and p

47 specific mutations in the T4 rII gene and on forward mutation in the T4 rI gene.

48 ion assay to show that the mean frequency of forward mutations in primary mammary adenocarcinomas ari

49 In conclusion, our study puts forward mutations in RCBTB1 as a cause of autosomal-rece

50 t mutations can account for up to 99% of all forward mutations inactivating the 4-kb LYS2 gene.

51 ion, implying that impairment of [PSI(+)] by forward mutations is due to altered ability of the ATPas

52 Viral evolution was mainly driven by forward mutations located inside CTL epitopes restricted

53 again tested conditionally on the number of forward mutations mapped on the sequence genealogy.

54 ich is tested conditionally on the number of forward mutations mapped on the sequence genealogy.

55 transgene that selects for phage containing forward mutations only in the lambda cII gene, using an

56 This can be attributed largely to the low forward mutation rate and the propensity for closely lin

57 The forward mutation rate in this system is 1.4 x 10(-5) mut

58 as corroborated by a 12-fold decrease in its forward mutation rate.

59 aize (Zea mays) afford the advantage of high forward mutation rates but pose a challenge for identify

60 L/F116Y/Q151M (VILYM) RTs, for their overall forward mutation rates in an M13 gapped-duplex assay tha

61 When the forward mutation rates were measured via a gap-filling a

62 in vitro tropism (residues 317 and 321) and forward mutation residues (residues 399, 460, 553, and 5

63 we used the ampD system to observe the true forward-mutation sequence spectrum of DSBR-associated st

64 into the mechanism of TAM, we obtained LYS2 forward mutation spectra under low- versus high-transcri

65 A defect in mug also has little effect on forward mutations, suggesting that Mug does not play a r

66 Finally, we summarize those aspects of T4 forward-mutation systems which are relevant to optimal c

67 ant displays a higher rate of CAN1S to can1r forward mutations than the ogg1Delta or msh2Delta single

68 in DNA repair capacity, approximately 50% of forward mutations that arise in the CAN1 gene under high

69 act instability phenotype and a high rate of forward mutations to canavanine resistance that result p

70 e reversions arose significantly faster than forward mutations, with the most rapidly reverting mutat