ライフサイエンスコーパス: freeze-thaw

1 and mDia2 lose >75% nucleation activity upon freeze-thaw.

2 n membranes pretreated by hypotonic wash and freeze-thaw.

3 aling rat hearts, injured 1 wk previously by freeze-thaw.

4 induced by the volume change of water during freeze-thaw.

5 effect, and are resistant to multiple 24 hr freeze-thaws.

6 emonstrates >98% reduction in activity after freeze-thawing.

7 nd encapsulation of ATP or other reagents by freeze-thawing.

8 One cycle of freeze-thaw (-70 degrees C) did not significantly reduce

9 oprotectants for cod fish mince subjected to freeze-thaw abuse.

10 ile maximizing salt extractable protein from freeze-thaw abused fish mince, providing similar or bett

11 Freeze-thaw and cell lysis experiments, along with actin

12 After fractionation by freeze-thaw and Fehling treatments of an alkaline extrac

13 cteristics in terms of pasting, rheological, freeze-thaw and swelling behaviour, were investigated.

14 LPI and investigates the effects of repeated freeze-thawing and oxidation on the anti-HIV activity of

15 Freeze-thawing and pasteurization increased the milk lip

16 After freezing-thawing and sonication, the resultant larger li

17 , wheat, barley and oat plants that had been frozen, thawed and allowed to resume growth under contro

18 nces of different storage (room temperature, frozen, thawing and refreezing) and buffer conditions on

19 hearts of adult inbred rats were injured by freeze-thaw, and 3-4.5 x 10(6) neonatal skeletal muscle

20 iplicate in phosphate buffer, ultrasonified, freeze-thawed, and clarified by centrifugation.

21 ossible greater sensitivity, stability after freeze/thaw, and lower cost, thus facilitating multicent

22 nctional integrity throughout the process of freeze-thawing (at -25 degrees C).

23 /9 mast cells cultured in vitro with live or freeze-thawed B. burgdorferi spirochetes undergo low but

24 ocyanins antioxidant capacity and texture of frozen/thawed blueberries.

25 lood mononuclear cells by cocultivation with freeze-thawed Borrelia burgdorferi spirochetes (Bb/FT).

26 f fragments with the N terminus ARGSVIL from freeze-thawed bovine nasal cartilage using the monoclona

27 Incubation of thin frozen-thawed brain sections with calcium resulted in ca

28 g were determined following preincubation of frozen-thawed brain tissue sections at 0 or 35 degreesC.

29 Although collagen in freeze-thawed cartilage depleted of aggrecan was complet

30 orcine ACVs mineralized similarly in situ in freeze-thawed cartilage.

31 ACVs in agarose gels and by ACVs in situ in freeze-thawed cartilage.

32 However, repeated freeze-thawing caused a more than 10% monomer loss.

33 ed by using a hexanol-detergent treatment of freeze-thawed chlorosomes.

34 es significantly augment the permeability of frozen-thawed coal masses.

35 The cooked gel of freeze-thawed control had 39% expressible moisture after

36 c amino acids significantly increased in the freeze-thawed crude fecal samples, suggesting a release

37 Frozen-thawed cucumber hypocotyl segments were strained

38 Results on fresh and frozen-thawed cutlets were compared to assess this way o

39 tical difference was found between fresh and frozen-thawed cutlets.

40 profiles resulting in increased warming and freeze-thaw cycle (FTC) frequency pose great ecological

41 The timing of the seasonal freeze-thaw cycle of arctic lakes affects ecological pro

42 included 24-h autosampler stability and one freeze-thaw cycle stability for the extracts.

43 at 24 h, but not at 6 or 12 h, and with one freeze-thaw cycle, all changes were within the range of

44 ns were tested at a 1:5 dilution and after a freeze-thaw cycle, respectively.

45 valuate storage duration-, temperature-, and freeze-thaw cycle-induced metabolic changes in crude sto

46 ble in the presence of thiolated DNA after a freeze-thaw cycle.

47 oxia or 500 microM N-methyl-D-aspartate or a freeze-thaw cycle.

48 Ps) and miRNA levels, and show that a single freeze/thaw cycle of plasma dramatically increases the n

49 We thus examined the effects of a single freeze/thaw cycle on microparticles (MPs) and miRNA leve

50 at -80 degrees C for 5years and to multiple freeze thaw cycles.

51 of control temperature (2 degrees C), daily freeze-thaw cycles (2 to -4 degrees C) and constant free

52 ty after 14 days at 37 degrees C), iterative freeze-thaw cycles (3.4-fold post four-cycles), and lyop

53 odispersion was stable against the effect of freeze-thaw cycles (no phase separation observed).

54 Thus, worms exposed to combined effect of freeze-thaw cycles and 4-NP suffer higher consequences,

55 remarkably stable in whole milk after three freeze-thaw cycles and for up to 30 h at room temperatur

56 enaturation, NAM samples were subjected to 7 freeze-thaw cycles between -20 degrees C and 4 degrees C

57 howed that combined effect of 4-NP and daily freeze-thaw cycles can cause higher mortality to worms a

58 In comparison to drying, freeze-thaw cycles created additional preferential flow

59 y of the refrigerated or frozen lysates, and freeze-thaw cycles did not adversely impact the quality

60 These forms emerge because freeze-thaw cycles drive an interplay between two feedba

61 thanol, also at -48 degrees C, with multiple freeze-thaw cycles for the extraction of metabolites.

62 Overall, the freeze-thaw cycles increased the mobilization of metal c

63 , understanding and predicting the effect of freeze-thaw cycles is important in environmental science

64 bation duration and temperature and repeated freeze-thaw cycles on HIV RNA recovery were analyzed.

65 We examined the effect of freeze-thaw cycles on the mobilization of cesium and str

66 ombinations and the effect of sonication and freeze-thaw cycles on the reproducibility, chemical shif

67 re not significantly changed during multiple freeze-thaw cycles or purification through sucrose gradi

68 4 degrees C for 3 days, -20 degrees C for 3 freeze-thaw cycles over 3 days, and on the autosampler.

69 Further, exposure to freeze-thaw cycles resulted in higher concentrations of

70 correlation with coal permeability, and the freeze-thaw cycles significantly augment the permeabilit

71 odium azide and paracetamol) or subjected to freeze-thaw cycles to induce cell death by a non-chemica

72 The potential of freeze-thaw cycles to release colloids and colloid-assoc

73 leptodactylus) muscle subjected to different freeze-thaw cycles was investigated.

74 Two freeze-thaw cycles were performed.

75 Due to global warming it is predicted that freeze-thaw cycles will increase in Arctic and cold temp

76 differences in clotting times, the number of freeze-thaw cycles, and different trypsin/protein ratios

77 stant in plasma exposed to room temperature, freeze-thaw cycles, and long-term frozen storage.

78 als, the effects of freezing time, number of freeze-thaw cycles, and the moisture content of coal wer

79 Samples were subjected to three freeze-thaw cycles, and total interleukin-1 receptor ant

80 atory, including long-term storage, multiple freeze-thaw cycles, different collection tubes, and the

81 red assembly reaction is driven by iterative freeze-thaw cycles, even in the absence of external acti

82 temperature, permeabilization with saponin, freeze-thaw cycles, sonication, or extrusion.

83 ambient and -20 degrees C and after multiple freeze-thaw cycles.

84 degradation after sonication, vortexing, and freeze-thaw cycles.

85 one week at +30 degrees C and following four freeze-thaw cycles.

86 ing isolates following multiple passages and freeze-thaw cycles.

87 table and shows no syneresis even after five freeze-thaw cycles.

88 m temperature for up to 24 h and after three freeze-thaw cycles.

89 on, and showed no loss of activity after six freeze-thaw cycles.

90 lts were reproducibly performed over several freeze-thaw cycles.

91 several hours and in CSF subjected to three freeze-thaw cycles.

92 red during nonrefrigerated transportation or freeze-thaw cycles.

93 el structure with higher digestibility after freeze-thaw cycles.

94 d stored at <-20 degrees C, avoiding further freeze-thaw cycles.

95 ophilising, blotting onto filter paper); and freeze-thaw cycles.

96 hydraulic conductivity (PLC) and exposed to freeze-thaw cycles.

97 blood was subjected to different numbers of freeze/thaw cycles and analyzed for the influence of sto

98 analysis of the study samples after multiple freeze/thaw cycles followed by a short-term benchtop sto

99 e, at 4 degrees C, and when exposed to three freeze/thaw cycles.

100 samples were exposed to different numbers of freezing/thawing cycles and separated into three batches

101 e their freshness according to the number of freezing/thawing cycles they exposed.

102 ical (centrifugation) and thermal stressors (freeze, thaw cycling).

103 Comparison to freezing time, freeze-thaw cycling caused much more damage to the coal

104 Freeze-thaw cycling stresses many environments which inc

105 pic phase transition coupled with repetitive freeze-thaw cycling.

106 cell viability and tissue architecture from freeze-thaw cycling.

107 that can protect lactate dehydrogenase from freeze-thaw damage by preventing the aggregation and den

108 The third variable studied, freeze-thaw damage resulting from high moisture content,

109 bility to protect lactate dehydrogenase from freeze-thaw damage.

110 sal lamina sheaths produced by mechanical or freeze-thaw damage.

111 on stimulation by BimEL, Bak-BH3 peptide, or freeze/thaw damage.

112 cy and using fresh samples, thereby avoiding freeze-thaw degradation of nucleotides.

113 method to evaluate the relationship between freeze-thaw embolism and conduit diameter across a range

114 success rate of in-vitro fertilization using frozen-thawed embryos now approaches that of using fresh

115 prostate cancer genotypes following a double freeze-thaw encounter.

116 isms of cell death that are attendant to the freezing-thaw encounter are clearly understood.

117 adults but a habitat subjected frequently to freeze-thaw episodes and bouts of pH, anoxic, and osmoti

118 Freeze-thaw events can affect plant hydraulics by induci

119 During winter, freeze-thaw events, snow depth, and soil freezing depth

120 reservation system and subjected to multiple freeze-thaw events.

121 riability during the winter (and likely more freeze/thaw events), had less extractable inorganic nitr

122 used to probe a Far Western blot of a yeast freeze/thaw extract (F/TE) that inhibited Hc binding to

123 Freeze/thaw extract (F/TE), a preparation of Hc yeast su

124 The Freeze-thaw (F-T) stability and turbidity of GCWS were a

125 ction from Leishmania infection, CpG-ODN and freeze-thawed (F/T) Leishmania major were coinjected int

126 be stored frozen prior to analysis, and the freeze/thaw (F/T) process introduces thrombin clots that

127 rank bone tissue if devitalized by standard "freeze & thaw" (F&T) cycles, associated with a significa

128 Frozen-thawed fillets showed two specific protein spots

129 ss of fish and distinguish between fresh and frozen-thawed fish fillets.

130 Gels of freeze-thawed FPH-2 and FPH-8 were similar to unfrozen c

131 ll as for environmental processes related to freeze-thaw fracturing.

132 new regions and subject others to a changing freeze-thaw frequency.

133 imate regions experience low temperature and freeze-thaw (FT) conditions in the winter.

134 dity after freezing and thawing and improves freeze-thaw (FT) survival.

135 hnique to study matrix mobility in fresh and freeze-thawed gelled yolk.

136 coli that evolved for 1000 generations under freeze-thaw-growth (FTG) conditions.

137 ased hepatocyte viability, individual viable frozen/thawed hepatocytes demonstrated clonal replicativ

138 c photosynthate input, wetting-event inputs, freeze-thaw impacts on substrate diffusion, aggregate tu

139 The traditional low pH pulse produced by freeze-thawing in mixed sodium phosphate buffer induces

140 n method and fractal dimension analyses, how freeze-thaw induced fractures in the coal was quantitati

141 Freeze-thaw induced fracturing coal by liquid nitrogen (

142 monitor the cavitation process and estimate freeze-thaw-induced PLC.

143 conduits (hydraulic diameter) showed higher freeze-thaw-induced PLC.

144 urther, we produced myocardial cell death by freeze-thaw injury to induce DCC.

145 The wetting events following freeze-thaw intervals mobilized about twice as many coll

146 ast, successive wetting events after 66 h of freeze-thaw intervals mobilized greater amounts of collo

147 nt SCE-inducing factor(s), which can survive freeze-thawing, is heat labile and also can be inhibited

148 In vitro assays on frozen-thawed leaf sections revealed that recombinant Zm

149 IL-10 and added B. burgdorferi spirochetes (freeze-thawed, live, or sonicated) or lipidated outer su

150 DC presentation of freeze-thaw lysate material derived from (either autolog

151 A series of bulk antigenic formats (freeze-thaw lysate, trifluoroacetic acid lysate, extract

152 506-binding protein (FKBP) and purified from freeze-thaw lysates in high yield by affinity chromatogr

153 We find that freeze-thaw lysates of human endothelial cells (EC) incr

154 RNA polymerase subunits in lysozyme-freeze-thaw lysates of minicells were associated with mi

155 tumor preparation: irradiation, boiling, or freeze thaw lysis for DC priming.

156 Freeze-thaw lysis appeared to inhibit CTL activity in vi

157 6+/-2 (n = 2), roughly 3-fold lower than for freeze-thaw lysis.

158 The freeze-thaw method clearly improved the detection of vol

159 ove the detection of volatiles in insects, a freeze-thaw method was applied to insect samples before

160 resulting from an individual cell lysed by a freeze-thaw method, gave an average signal-to-noise rati

161 ochondria and electron transport activity in freeze-thawed mitochondrial membrane fragments in a dose

162 Fresh and frozen/thawed mouse hepatocytes were transferred separat

163 ntage over the reported poor permeability of freeze-thawed nerve grafts.

164 Following freeze-thaw of 247 specimens, indeterminate rates were 1

165 Freeze-thawing of both bevacizumab and placebo samples l

166 The evaluation of freshness and freeze-thawing of fish fillets was carried out by assess

167 ng the permeability of mitochondria by quick freeze-thawing of fresh homogenates just before assay di

168 For applications from food science to the freeze-thawing of proteins it is important to understand

169 ng vaccinia virus) or mechanical cell death (freeze/thaw of ovalbumin-expressing cells), tissue cultu

170 Freezing-thawing of PBPC had no effect on porcine CFUs b

171 fter cell death) was produced in vivo by the freezing-thawing of rat thymus and in cell culture of Ju

172 o three batches, namely (i) fresh, (ii) once frozen-thawed (OF) and (iii) twice frozen-thawed (TF) sa

173 The effect of freeze-thawing on cytosolic lactate dehydrogenase and ly

174 ric points, distinguished fresh fillets from frozen-thawed ones.

175 amine:DOPC (1:1, mol:mol) prepared by either freeze-thaw or reverse-phase evaporation methods, neithe

176 s for OT-1 CTLs, whereas DCs stimulated with freeze-thawed or boiled tumors did not stimulate IFN-gam

177 In human platelet lysates prepared by freeze-thawing or by the addition of nonionic detergent,

178 Freeze-thawing or other mishandling can further increase

179 Levels were unaffected by freezing/thawing or prolonged storage and did not displa

180 normal cells or cells killed by irradiation, freeze thaw, or osmotic shock.

181 Orthotopic reimplantation of frozen/thawed ovarian cortical strips is a well tolerate

182 The effect on RNA quality of freeze-thawing peripheral blood cells and storage in pre

183 idation products and the compound present in freeze-thawed plasma suggests that gamma-linolenic acid

184 In both species, the freeze-thaw plus water-stress treatment caused more embo

185 combination with the N-terminal domain via a freeze-thawing procedure.

186 ure suggest that uncertainty associated with freeze-thaw processes as well as soil textural effects o

187 nutrient availability, cold temperature and freeze-thaw processes, UV and radical exposure, and evap

188 gas-based cryoablation system with a double-freeze-thaw protocol was used to treat cancers in outpat

189 rotoplasts, it increased the cryosurvival of frozen-thawed protoplasts by 24% over untreated or BSA-t

190 otein complex was maximized by extraction of frozen-thawed protoplasts with a pH 8.8 carbonate buffer

191 relationship between Px and Df to model the freeze-thaw response in conifer species.

192 can differentiate between fresh skinless and frozen-thawed sea bass (Dicentrarchus labrax) fillets us

193 markers of differentiation between fresh and frozen-thawed sea bass fillets.

194 le preparation is investigated by repeatedly freezing/thawing short strands followed by matrix-assist

195 nsfer of apoptotic (irradiated) or necrotic (freeze thaw) splenocytes.

196 recovery studies, autosampler stability, and freeze-thaw stability.

197 This starch is extremely freeze-thaw-stable and shows no syneresis even after fiv

198 We have now created a freeze-thaw-stable potato starch by alteration of starch

199 es, horse mackerels and chub mackerel during frozen/thawed storage.

200 cells from the recipients were cultured with frozen/thawed stored donor cells or major histocompatibi

201 alpine timberline are exposed to drought and freeze-thaw stress during winter, which induce potential

202 ope with harsh conditions, including extreme freeze-thaw stress.

203 aria K2) on the protection of membranes from freeze-thaw stress.

204 ure after an 8.5% cook loss, whereas gels of freeze-thawed SuSo, FPH-2 and FPH-8 had significantly lo

205 A multiplexed freeze/thaw switching principle and a distribution netwo

206 mp plus a set of multiplexed liquid nitrogen freeze/thaw switching valves are employed for liquid han

207 t addition of SMP resulted in an increase in freeze-thaw syneresis and reduction in starch granule si

208 us of PapA and PapE was mimicked in vitro by freeze-thaw techniques and resulted in the formation of

209 (ii) once frozen-thawed (OF) and (iii) twice frozen-thawed (TF) samples, in order to perform the fres

210 pared with controls using tetanus toxoid and frozen/thawed third-party cells with no human leukocyte

211 ks) and old (24 months) rats were repeatedly freeze-thawed to kill the cellular component of the vess

212 is model was challenged using a subset of 23 freeze-thawed training samples.

213 After fractionation by freeze-thaw treatment of an aqueous extract (BW), a frac

214 obicity of control and FPH-2 increased after freeze-thaw treatment, while that of FPH-8 did not chang

215 ere compared to control NAM before and after freeze-thaw treatment.

216 ed NAM protein structure and function during freeze-thaw treatment.

217 enerated with versus without superimposing a freeze-thaw treatment.

218 and protect the liposomes from fusing after freeze-thaw treatment.

219 death or mechanical cell death, elicited by freeze/thaw treatment of cell suspensions, yielded GRP94

220 aggregation, oxidant etching, and repetitive freeze/thaw treatment-because of the presence of their m

221 Drying and freeze-thaw treatments, respectively, increased and decr

222 ptobrevin 2 immunoprecipitates obtained from frozen-thawed Triton X-100 extracts, which were greatly

223 Freeze-thaw valves (FTVs) can provide a low cost, low co

224 nt of damage caused by each of the different freeze-thaw variables were empirically regressed.

225 To evaluate the different freeze-thaw variables which modify the mechanical proper

226 hosphatidyl serine-coated microtiter plates, frozen thawed washed platelets, activated partial thromb

227 e and the effect on the metabolic profile of freeze-thawing were examined.

228 Freeze-thawing, which involves a mild denaturing step, m

229 ting effect occurred if the T9-C2 cells were freeze-thawed, x-irradiated, or treated with mitomycin-C