ライフサイエンスコーパス: frozen

1 ut not in diploids that have been cloned and frozen.

2 exists for a short time before the sample is frozen.

3 uberculosis for 6 days, and cellular RNA was frozen.

4 ngth near the transition remains effectively frozen.

5 wab); 43/71 BS specimens had been previously frozen.

6 ned with hematoxylin and eosin, and/or fresh frozen.

7 ed at room temperature for 96 hours and then frozen.

8 Upon enrichment with frozen (13%) and freeze-dried curly kale (3%), the natur

9 h converge to a single six-line pattern in a frozen 2-MeTHF glass sample, indicating a unique iron en

10 Dry-cured loins elaborated from frozen (-20 degrees C/20 weeks)/thawed longissimus dorsi

11 Three lots of custom-made frozen 96-well polystyrene microtiter plates were used a

12 ode likely results from the combination of a frozen accident, i.e., the deleterious effect of codon r

13 of the code via proto-tRNA duplication, and frozen accident.

14 are unrealized could be due to evolutionary frozen accidents or optimization, though this optimizati

15 One hundred three frozen and 10 fresh CMV DNA-positive plasma samples from

16 re enrolled in the extension (11 eyes with a frozen and 15 eyes with a fresh carrier graft).

17 this was the only form in 93 of 103 (90.3%) frozen and all 10 fresh samples tested using DNase-CMV-q

18 ran 4 the conformational equilibria could be frozen and assigned.

19 ed from native potato starch, and afterwards frozen and defrosted.

20 re recovered in comparable yields from fresh-frozen and FFPE preserved tissues of rodents treated wit

21 hniques require separate sample collections (frozen and fixed) which may result in discrepancies due

22 on the base of apple juice with addition of frozen and freeze-dried curly kale leaves.

23 uspended in a thin aqueous layer are rapidly frozen and imaged at cryogenic temperature in the transm

24 , greenhouse (all liquid seas) and icehouse (frozen and liquid).

25 Frozen and paraffin-embedded tumor tissues of different

26 crystallinity more than storage time at both frozen and room temperature.

27 read were baked and stored at refrigeration, frozen and room temperatures with analysis over a period

28 hat viable organoids can be grown from flash-frozen and thawed tissue and from bulk tissues slowly fr

29 One half was frozen and used to measure the tau prion content, while

30 d type of stools used (for example, fresh or frozen), and duration of stool conservation (67%).

31 which a whole mouse brain is embedded, flash frozen, and cryosectioned in preparation for mass spectr

32 transcriptional programs of tumor nuclei in frozen archival tissue samples.Single cell RNA sequencin

33 fresh, prospectively collected (n = 914) and frozen, archived (n = 489) fecal specimens.

34 and are therefore not limited to perennially frozen areas.

35 Genetic decoding is not 'frozen' as was earlier thought, but dynamic.

36 Crude fecal samples frozen at -20 degrees C manifested elevated amino acids

37 Insect samples were first frozen at -80 degrees C for 10 min and then thawed at 25

38 oscopy in Germany from 2005 through 2010 and frozen at -80 degrees C until analysis.

39 o far, to stabilize PSM, it must be remained frozen at -80 degrees C.

40 -phenyl-3-silatetrahydropyran 3 could not be frozen at 100 K and proved to be heavily one-sided (if n

41 e six-membered-ring interconversion could be frozen at 103 K and the present conformational equilibri

42 aboration, which in the present analysis was frozen at Feb 8, 2015.

43 cryoprotectants are thermally unstable when frozen at higher storage temperatures, e.g. -80 degrees

44 Whole brains were snap-frozen at necropsy and were subsequently sectioned prior

45 SAVs frozen at speciation might maintain protein function, wh

46 se constantly changing intermediates can be 'frozen' at controlled time points into particles with va

47 l diagnostic samples, some of which had been frozen before use; it was not possible to test the perfo

48 bond was registered at room temperature and frozen below -30 degrees C with the proton fixed on the

49 Three anthocyanins were identified in frozen berries and RW-dried powder: cyanidin 3-glucoside

50 Cold maceration of frozen berries without enzyme addition gave the highest

51 ze-etching (FE) procedure to prepare rapidly frozen biological cells or tissues for electron microsco

52 oarray gene expression data of matched fresh frozen biopsies as a gold standard.

53 ive proteomics and phosphoproteomics on snap-frozen biopsies of hepatocellular carcinoma (HCC) and ma

54 se events associated with placement of fresh-frozen bone allografts (FFBAs) during alveolar ridge aug

55 Immunoblot analysis of fresh frozen brain tissues revealed that tau was present in th

56 vailable, enabling the retrieval of specific frozen brains through the UK Medical Research Council Br

57 We also analyze 416 nuclei from a frozen breast tumor sample and 380 nuclei from normal br

58 n that dominates in the compounds, and that "frozen" bulk diffusion leads to unique composition chara

59 finite conduction is achieved from a charge frozen by Coulomb interaction.

60 as permafrost thaw exposes immense stores of frozen carbon (C) to microbial decomposition.

61 hich limits our understanding of the fate of frozen carbon in a warming world.

62 , with 19 eyes randomized to fresh and 18 to frozen carrier grafts.

63 How does a frozen cell membrane fracture?

64 ritus approximately two hours after eating a frozen Citrus unshiu.

65 genetic variations were identified in fresh frozen clinical specimens by Illumina RNA-sequencing, th

66 nalytical parameters with a properly handled frozen cohort is necessary to ensure a high degree of co

67 with recurrent or refractory CDI, the use of frozen compared with fresh FMT did not result in worse p

68 Frozen cores were sectioned into 1 cm slices, and trace

69 Fresh and frozen corneal donors offer similar clinical outcomes wh

70 long-term clinical outcomes of fresh versus frozen corneal graft carriers for the Boston Keratoprost

71 Conventional frozen cryopreservation (CFC) is currently the gold stan

72 ormalin-fixed, paraffin-embedded sections or frozen cryosections for immunohistochemistry.

73 e potato starch (1, 4, 10, 18 or 30 g/100g), frozen, defrosted and dried.

74 ads from five embalmed donors and five fresh-frozen donors were compared using Raman microspectroscop

75 Enceladus suggests that the grains formed as frozen droplets from a liquid water reservoir that is, o

76 rfaces is an inter-droplet phenomenon, where frozen droplets harvest water from neighboring supercool

77 nt, leaving behind concentrated, kinetically frozen drug micelles containing minimal solubilizing exc

78 imation of the latter, the samples were kept frozen during the whole experiment using a nitrogen cool

79 lation and all subsequent separate fresh and frozen embryo transfers.

80 We compared fresh frozen tissue, fresh frozen endoscopic brushings, and the Cytosponge device f

81 Snap-frozen FCDIIb specimens were tested for HPV DNA using th

82 Moreover, fresh/frozen fish consumption, longer birth intervals, and Slo

83 ride-containing raw materials (e.g., breaded frozen fish products) can lead to the formation of fatty

84 gar-based cryoprotectants currently used for frozen fish products.

85 rease in the international trade of packaged frozen fishery products, this study used DNA barcoding t

86 on the clinical resolution was 75.0% for the frozen FMT group and 70.3% for the fresh FMT group (diff

87 s with clinical resolution was 83.5% for the frozen FMT group and 85.1% for the fresh FMT group (diff

88 A total of 219 patients (n = 108 in the frozen FMT group and n = 111 in the fresh FMT group) wer

89 Given the potential advantages of providing frozen FMT, its use is a reasonable option in this setti

90 ntention-to-treat (mITT) population and 178 (frozen FMT: n = 91, fresh FMT: n = 87) in the per-protoc

91 Broccoli by-products from frozen-food industry account for 45% of the initial broc

92 urpose, the volatile profiles of wheat bread frozen for 1, 2 and 4weeks were analysed employing solve

93 Samples were tested fresh or were frozen for later testing within 8 h after the bottles we

94 ortant as the fertilization success of sperm frozen for less than 1 month was similar to that frozen

95 on, specimens are either used immediately or frozen for use at a later time.

96 erved in plastic-embedded resin or in plunge-frozen form, can be investigated in three dimensions by

97 hese Golgi stacks, we examined high-pressure frozen/freeze-substituted alfalfa root cap cells with el

98 osition and antioxidant properties of stevia frozen fresh and dried leaves, and to define the best gr

99 ting dried samples with higher contents than frozen fresh ones, while the latter better retained toco

100 The zebrafish, frozen (fro) mutant was identified as one of a group of

101 tent ranged from 43.84 to 326.29 mg GAE/100g frozen fruit.

102 tely 93.8% of anthocyanins from the original frozen fruits, as assessed by the pH-differential method

103 DNA was extracted from snap-frozen gastric corpus tissues and 16S rRNA was sequenced

104 net has been through phases of snowball (all frozen), greenhouse (all liquid seas) and icehouse (froz

105 a baseline of counting fingers, whereas the frozen group improved to 20/400 from a baseline of hand

106 use of DSCMs shows promising correlation to frozen histologic analysis, but image quality was affect

107 ty of detecting NMSC using DSCMs vs standard frozen histopathologic specimens.

108 proved more discriminatory than spectra from frozen homogenized berry samples.

109 ing and copy number variant analysis on 1511 frozen human brains with a diagnosis of Alzheimer's dise

110 eous ex vivo tumor samples, sections of snap frozen human breast cancer murine xenografts were subjec

111 oman-inhibited AChE solubilized from 8 mL of frozen human erythrocytes, yielding a quantity sufficien

112 ference Material (SRM) 1950, "Metabolites in Frozen Human Plasma", against benchmark consensus mean c

113 Material (SRM) 972a Vitamin D Metabolites in Frozen Human Serum as a replacement for SRM 972, which i

114 st use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolut

115 rticle approach to helical reconstruction of frozen hydrated native cardiac TFs under relaxing and ac

116 Developments in the electron microscopy of frozen hydrated samples (cryo-electron microscopy) are p

117 used ion beam (cryo-FIB) for the thinning of frozen-hydrated biological specimens enabled cryo-electr

118 the 3D structure of a porous material and a frozen-hydrated marine cyanobacterium.

119 stem that streamlines the entire handling of frozen-hydrated samples from the vitrification process t

120 mples such as intact cells in a near-native, frozen-hydrated state to macromolecular resolution ( app

121 als only a few hundred nanometers thick in a frozen-hydrated state.

122 molecular complexes inside cells in a native frozen-hydrated state.

123 tilt-corrected 2-nm three-dimensional map of frozen-hydrated tarantula thick filament.

124 opa (and possibly others) and the liquid and frozen hydrocarbon oceans on Titan probably represent th

125 e set from each sample collection method was frozen immediately, and a second set was incubated at ro

126 When compared with fecal samples that were frozen immediately, the ICCs were below 0.60 for the met

127 We found that while flash-frozen iMNs are not suitable for ATAC-Seq, the assay is

128 For (13)C spins on biomolecules frozen in a glassy matrix, electron decoupling reduces t

129 rchers the ability to observe macromolecules frozen in action in situ, but a primary challenge with t

130 d thawed tissue and from bulk tissues slowly frozen in DMSO supplemented media.

131 perception that mutant KRAS is persistently frozen in its active GTP-bound form may not be accurate.

132 optimal cutting temperature (OCT) medium and frozen in molds.

133 o different categories: in one the system is frozen in the phase space as the applied gain increases,

134 y of several trophically interacting taxa is frozen in time by exceptional preservation.

135 toughness that is extremely sensitive to the frozen-in configurational state.

136 uations are too weak, stress heterogeneities frozen-in upon solidification can still partially relax

137 l tensile stresses in the graphite that are 'frozen-in' following processing.

138 nited States blood samples and 54 previously frozen Kenyan blood samples.

139 geochronology and geochemistry from both the frozen lava and the cogenetic enclaves they host from th

140 er rigid orientation of the POTRA domains in frozen liquid solution.

141 based on the drilling of holes in a block of frozen liver homogenate, providing easy cryo-slicing and

142 arkers were assessed in parallel using fresh-frozen lung tissue from sibling rats of the same cages.

143 ting Coulomb spin liquid with defect-induced frozen magnetic degrees of freedom.Experimental studies

144 fresh plant tissue, formalin-fixed and fresh frozen mammalian tissue, fixed cells and biofluids.

145 with inward solidification causing not fully frozen mass to be displaced toward the unsolidified drop

146 our patterns due to the random and initially frozen material imperfections which act as nucleation po

147 ion signature-using gene expression in fresh frozen material.

148 (VIII) anion, OsO3 N(-) , in low-temperature frozen matrices results in nitrogen-oxygen bond formatio

149 eam, as a complex food system, consists of a frozen matrix containing air bubbles, fat globules, ice

150 ell lines (UISO, MCC13, and MCC26) and fresh frozen MCC tumors.

151 allowed to relax to the resting E0 state in frozen medium at a temperature below the melting tempera

152 I discovered that all frozen membranes tend to split along weakly bonded lipid

153 Motion-frozen (MF) registration algorithms were applied to elec

154 Edible, surfactant-stripped, frozen micelles are formed from pheophytin (demetallated

155 Testing was performed in frozen microtiter panels according to the Clinical and L

156 derived from the IMS investigation of fresh frozen mouse liver and rabbit adrenal gland tissue secti

157 e well-preserved fossils were recovered from frozen "muck" deposits (organic-rich silt) exposed withi

158 Patients were randomly allocated to receive frozen (n = 114) or fresh (n = 118) FMT via enema.

159 after deviation from metastability or can be frozen on returning from nonequilibrium to equilibrium.

160 zed individually to receive a B-KPro using a frozen or a fresh corneal graft carrier on the basis of

161 ured with no relapses until the database was frozen or death.

162 affiliation by gene expression profiling on frozen or formalin-fixed paraffin-embedded tissues.

163 se changes, along with thawing of previously frozen organic material, can alter the form and magnitud

164 Heating of atropoisomers (i.e. "frozen-out" conformational isomers) in solution leads to

165 We obtained frozen pancreatic tumor specimens from patients and meas

166 e substrate do not occur simultaneously: The frozen phase boundary moves inward from the droplet free

167 od cell (3 [0, 6] vs 4 [1, 7] P = 0.003) and frozen plasma (6 [2, 10] vs 6 [2, 12], P = 0.032) transf

168 is used to justify preprocedure use of fresh frozen plasma (FFP) and/or platelets (PLT).

169 ractice of a high transfusion ratio of fresh frozen plasma (FFP) to red blood cells (RBCs) has spread

170 We assessed the safety and efficacy of fresh frozen plasma (FFP) versus prothrombin complex concentra

171 and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery.

172 Higher fresh frozen plasma ratios (> 1:2) were not associated with in

173 injection mass spectrometry was performed on frozen plasma samples obtained prior to randomization, a

174 eeding disorder treated by infusion of fresh-frozen plasma, plasma-derived FVII concentrates and low-

175 Fresh frozen plasma-to-packed RBCs and platelets-to-packed RBC

176 The benefits of higher ratios of fresh frozen plasma-to-packed RBCs and platelets-to-packed RBC

177 ltural group selection under the view of the frozen plasticity theory and the different explanatory p

178 to fresh single mouse cortical cells and to frozen post-mortem human cortical nuclei, matching the p

179 uronal and non-neuronal nuclei isolated from frozen postmortem human brain by fluorescence-activated

180 processing factors calculated (fresh product/frozen product) are lower than 1, indicating a clear inf

181 oatings can improve the quality of fresh and frozen products by retarding microbial growth, reducing

182 indicate the application in refrigerated and frozen products.

183 The census dates on which the data were frozen ranged from Dec 31, 2009, to Dec 1, 2014.

184 on average 0.66 Anisakis per untreated (non-frozen) raw or marinated anchovy meal.

185 AChE was solubilized from frozen RBC by addition of 1% Triton X-100.

186 57 specimens collected prospectively and 785 frozen, retrospective samples, were evaluated.

187 ative stx1 or stx2 results were reported for frozen, retrospectively tested specimens.

188 e fluorophore-labeled structures in the same frozen sample.

189 red-shifted absorption tails of anilines in frozen samples (while having a marginal overlap with the

190 it is difficult to perform this technique on frozen samples because freezing cells before extracting

191 calculated from pancreatic transection line frozen samples by a pathologist.

192 5 nm of the absorption maxima of anilines in frozen samples compared to those in liquid aqueous solut

193 rely on viable cells allowing measurement of frozen samples in a remote setting from the clinic.

194 h-numerical-aperture objective lens to image frozen samples in their native state.

195 for hematopoietic stem cell transplantation, frozen samples of cord blood were thawed and the purity

196 are concordant with ChIP-seq data from fresh-frozen samples of the same tumors.

197 Frozen samples were shipped to a reference biosafety lev

198 mitted the same data reproducibility as with frozen samples, and yielded higher recovery of nucleic a

199 ein was precisely isolated from the adjacent frozen SAN tissue blocks using a 16G biopsy needle.

200 The method was applied in frozen sea bream samples stored at 0-4 degrees C.

201 he possession of water nor having liquid and frozen seas are unique to Earth (Figure 1).

202 s to test the hypothesis that intraoperative frozen section (FS) and re-resection results to achieve

203 red 2.4 minutes compared to 26.5 minutes for frozen section (P < 0.001) and it proved more accurate t

204 re calculated per group (Sens, Spec, AUROC): frozen section = 86%, 96%, 0.96 (n = 9); cytology = 91%,

205 rgeons rely on touch preparation cytology or frozen section analysis to assess tumour margin status i

206 positive by DESI-MSI/Lasso, but negative by frozen section analysis.

207 Pooled data suggest that frozen section and cytology have the greatest diagnostic

208 ere blinded to the correlating gold standard frozen section diagnoses, independently reviewed the DSC

209 The developed method showed agreement with frozen section evaluation of specimen margins in 24 of 3

210 (P < 0.001) and it proved more accurate than frozen section in diagnosing lung adenocarcinomas.

211 n-destructive alternative to intra-operative frozen section pathology, using prostate cancer as an in

212 h paraffin section control (21.7%), WLE with frozen-section control (19.3%), and excision without mar

213 atrix for spiked drug standards which can be frozen, sectioned and subsequently analyzed for the gene

214 ive cohort study of 3148 cases pertaining to frozen sections associated with the staged excision of N

215 ccurate identification of prostate cancer in frozen sections at the time of surgery can be challengin

216 Immunostaining of left ventricular frozen sections before and 8 h after CLP revealed the pr

217 viewed, 60 showed inflammation in histologic frozen sections from an excision specimen that was follo

218 in cancer (NMSC), inflammation in histologic frozen sections has been found to occasionally presage t

219 asionally presage the detection of tumors in frozen sections of adjacent excision specimens.

220 phic surgery of NMSC with the examination of frozen sections, histologic inflammation is modestly pre

221 Maintaining the fertilizing potential of frozen semen as it is manipulated, transported and store

222 We show that when frozen semen is mishandled, characteristics of semen bio

223 Frozen serum samples were subjected to sclerostin quanti

224 ix study-related complications that were all frozen shoulders (in two patients in each group).

225 Samples stored frozen since 2000 included those with 2 of the 3 highest

226 nt to generate a dynamic sequence instead of frozen snapshots of the process.

227 Perennially frozen soil in high latitude ecosystems (permafrost) cur

228 Hitherto, ancient frozen soils have proved excellent in preserving DNA mol

229 melting procedure necessary to transform the frozen solid into an injectable solution containing the

230 Our brute-force process ejects the frozen, solid sample from the low-T, high-B polarizer, p

231 mafrost and increased mobility of previously frozen solutes to Arctic freshwaters.

232 electron-nuclear double resonance (ENDOR) in frozen solution (80 K) indicates distribution of the spi

233 f fructose 1,6-bisphosphate in a permanently frozen solution as followed over months.

234 copolymers were prepared by encapsulating a frozen solution of monomer B on the bottom of a reaction

235 of the diferric state does not represent the frozen solution structure and that a mono-mu-hydroxo dif

236 y [VO(Et2dtc)2] (1), in both solid-state and frozen solution.

237 e at cryogenic (liquid N2) temperatures, and frozen solutions as well as solid samples decompose rapi

238 Frozen solutions of these catalytic mixtures generally e

239 ductions of Xn-Hex(*-) yield EPR signals (in frozen solutions) that can be assigned to spin-spin inte

240 tributions of conformers, are similar in the frozen specimens used for cryo-EM and in the solution ph

241 An additional 55 retrospectively collected, frozen specimens were included to increase the number of

242 For both prospective and frozen specimens, the Xpert Norovirus assay showed posit

243 largely remained limited to fresh or rapidly frozen specimens.

244 s used as a measure of cell viability in the frozen state and this metric agreed well with the popula

245 heoretically predicted crossover into a spin-frozen state.

246 A "frozen" state with negligible motion of domain walls on

247 The development of a frozen stool bank of screened donor stool is an importan

248 ve stability of fish muscle during long term frozen storage (-10 degrees C) were evaluated using farm

249 After a 12-month frozen storage period, flavonoid, phenolic, and ascorbic

250 nderstanding, prediction, and control of the frozen storage process.

251 However, the impact of the frozen storage time on the aroma profile has not been st

252 Thus, a maximum of one week of frozen storage was recommended when using the solvent ex

253 pulp upon pasteurization, during 12months of frozen storage, and in butia nectar after a 3-month stor

254 fect of dietary vitamin E was independent of frozen storage, so these effects were analysed separatel

255 lity (TBARS) after processing and also after frozen storage.

256 g the stability of the gels during long-term frozen storage.

257 ccessibility of capsaicinoids was altered by frozen storage.

258 idation (P<0.01) and less colour loss during frozen storage.

259 tability of fresh and thawed lamb leg chops, frozen stored for 3, 6 and 9months.

260 method was applied for analysis of fresh and frozen strawberries purchased at local markets.

261 Electron tomography of high-pressure frozen synapses revealed a reduced fraction of membrane-

262 sualization and super-resolution imaging for frozen systems.

263 es significantly augment the permeability of frozen-thawed coal masses.

264 ss of fish and distinguish between fresh and frozen-thawed fish fillets.

265 In vitro assays on frozen-thawed leaf sections revealed that recombinant Zm

266 markers of differentiation between fresh and frozen-thawed sea bass fillets.

267 ocyanins antioxidant capacity and texture of frozen/thawed blueberries.

268 tes between organoids derived from fresh and frozen tissue and can be used to detect drug response of

269 nd DNA methylation data were generated using frozen tissue from the dorsolateral prefrontal cortex.

270 romatin accessibility profiles from archival frozen tissue samples and 50-mum sections, revealing the

271 ut, cost and the ability to process archival frozen tissue samples.

272 nched activity-based probes (qABPs) to fresh-frozen tissue samples.

273 In this method, fresh-frozen tissue sections are fixed, incubated with the pro

274 to near-quantitatively degrade PCs in fresh-frozen tissue sections.

275 required by histopathological examination of frozen tissue specimens.

276 The slow, DMSO frozen tissue yielded organoids with more accurate drug

277 (GGI) was previously developed, evaluated on frozen tissue, and shown to be prognostic in early breas

278 We compared fresh frozen tissue, fresh frozen endoscopic brushings, and th

279 e obtained from FFPE tumor tissues to detect frozen tissue-derived prognostic gene expression signatu

280 d the expression of 516 genes from published frozen tissue-derived prognostic signatures in 42 FFPE C

281 is study, to evaluate the gene expression of frozen tissue-derived prognostic signatures in FFPE CRC

282 s of cancer nuclei in parallel from fresh or frozen tissue.

283 an studies is often restricted because fresh-frozen tissues are not available.

284 h more accurate drug response than the flash frozen tissues, and thus bulk tissue should be preserved

285 man lung cancer tissue microarrays and fresh frozen tissues, we found that the overexpression of DNMT

286 detect drug response of organoids grown from frozen tissues.

287 and drug response of organoids derived from frozen tissues.

288 cancer and colon cancer cell lines and flash-frozen tumor lysates with a linear range of 0.05-2.0 fmo

289 en for less than 1 month was similar to that frozen up to 2 years (p > 0.05).

290 s used in pathology, we selected small fresh frozen uterine tissue samples to investigate how the tis

291 Vaccination teams and frozen vaccine were flown to the outbreak settings.

292 neity already in the cells from the original frozen vials from the same ATCC lot, however, STR marker

293 ectly and, in some cases, through uncovering frozen volatiles.

294 tivity and moisture content, while increased frozen water content and resulted in a softer bread crum

295 roperties (water activity, moisture content, frozen water content, amylopectin retrogradation) and (1

296 ad staling (crumb moisture loss, decrease in frozen water content, formation of amylopectin crystals,

297 Moisture and frozen water contents were not affected by formulation n

298 Frozen water oceans on the moons Enceladus and Europa (a

299 erformed on genomic DNA extracted from fresh-frozen whole blood and patient-matched tumor pairs from

300 By working with cells that have been rapidly frozen without the use of chemical fixatives, and imagin