ライフサイエンスコーパス: fucosylation

1 nnose residues and a Glc-Fuc disaccharide (O-fucosylation).

2 human alpha-1-acid-glycoprotein (for antenna fucosylation).

3 factor 1-alpha (HNF1A) as a key regulator of fucosylation.

4 unrecognized general defect in N-glycan core fucosylation.

5 ing Fcgamma receptor, independent of Fc core-fucosylation.

6 -glycan is essential for FUT8-catalyzed core fucosylation.

7 is, rather than differences in levels of XyG fucosylation.

8 ha(1,3)fucosyltransferase FucT-VII-dependent fucosylation.

9 carbohydrate core-2 branching or alpha(1,3)-fucosylation.

10 ligosaccharides, as well as a high degree of fucosylation.

11 are triantennary and trisialylated with core fucosylation.

12 y oligosaccharide that is influenced by core fucosylation.

13 on ADCC was dependent on the status of core fucosylation.

14 ylation while its absence signifies antennae fucosylation.

15 ly leads to ambiguous assignment of N-glycan fucosylation.

16 enzyme, significantly increased Treg surface fucosylation (66% vs 8%).

17 modification of Notch receptors by O-linked fucosylation, a reaction that is catalyzed by protein O-

18 se of sialylation alpha2-6, poly-LacNAc, and fucosylation, all known epitopes found in different tumo

19 escence (0-9 years) the levels of alpha(1-3)-fucosylation, alpha(2-3)-sialylation, and galactose sulf

20 osylation, suggesting that the level of core fucosylation also depends on the nature of the recombina

21 g the Lewis[x] determinant), alpha(1,6)-core fucosylation and a bisecting GlcNAc residue.

22 w for quantitative site-specific analysis of fucosylation and apply it to a comparison of hemopexin (

23 A pre-requisite for both core alpha1,6-fucosylation and beta1,4-galactosylation is the presence

24 a-galactose and are more diverse with higher fucosylation and better interaction with DC-SIGN [DC-spe

25 t human glycoprotein to determine changes in fucosylation and changes in sialylation that were in ver

26 35c1 overexpression causes elevated N-linked fucosylation and disrupts embryonic patterning in a tran

27 nique will allow dynamic imaging of cellular fucosylation and facilitate studies of fucosylated glyco

28 alyses showed that the IgG-Fc glycoform with fucosylation and fully galactosylation was an independen

29 During maturation (9-18 years) the levels of fucosylation and galactose sulfation continue to increas

30 in primary rat hepatocytes can increase core fucosylation and induce additional glycoform alterations

31 Secretion of ADAMTS17 requires O-fucosylation and its autocatalytic activity does not dep

32 o modification of host glycans by increasing fucosylation and mannose content, while decreasing sialy

33 Notch activity is regulated by both O-fucosylation and O-glucosylation, and Notch receptors co

34 Amounts of core fucosylation and of triantennary glycans increased subst

35 of antibodies with different combinations of fucosylation and sialylation and performed side-by-side

36 hypoxia also results in increased levels of fucosylation and sialylation.

37 axy8 exhibits increased XyG fucosylation and the occurrence of XyG fragments not pre

38 transporter, slc35c1 that promotes terminal fucosylation and thereby limits Wnt activity.

39 of GSP-6' and GSP-6" to P-selectin required fucosylation and, to a lesser extent, sialylation as wel

40 triantennary GlcNAc branching, and alpha1,6-fucosylation) and augmented by Asn(347) NeuAc-type sialy

41 h is here validated with human IgG (for core fucosylation) and human alpha-1-acid-glycoprotein (for a

42 the sequences with terminal sialylation and fucosylation, and addition of LacNAc repeat structures.

43 Commensal bacteria induce epithelial fucosylation, and epithelial fucose is used as a dietary

44 s of bi- and triantennary, core and terminal fucosylation, and mono- to trisialylation.

45 selectin ligand expression, general cellular fucosylation, and normal postnatal physiology is achieve

46 a virtually complete deficiency of cellular fucosylation, and variable frequency of intrauterine dem

47 Thus, rapid IEC fucosylation appears to be a protective mechanism that u

48 ng defects associated with enhanced N-linked fucosylation are due to diminished canonical Wnt signali

49 rotein sialylation and alpha-1,6-linked core fucosylation are observed following activation of the be

50 As increases in sialylation and fucosylation are prominent among cancer-associated modif

51 ms that regulate the induction of epithelial fucosylation are unknown.

52 last menstrual period, indicating more core fucosylation as well as possible changes in branching of

53 tyllactosamine units) with internal multiple fucosylation at GlcNAc.

54 lectin: (i) those having a single alpha1-->3 fucosylation at internal GlcNAcs but not at the penultim

55 cNAc and (ii) those having double alpha1-->3 fucosylation at internal GlcNAcs, excluding the penultim

56 plantation mouse embryo, as well as alpha1,3-fucosylation at multiple internal GlcNAc of unbranched p

57 rms at the N187 site of HPX, absence of core fucosylation at N882 and N911 sites of CFH, or a higher

58 mbin results from the specific difference in fucosylation at residue 155, which may result in differe

59 ort here a detailed study of the effect of O-fucosylation at Ser-60 on the structure of FVII EGF-1, i

60 ng of complex-type N-linked glycans +/- core fucosylation but does not process oligomannose- or hybri

61 In addition, the alpha1-3,-4 fucosylation, but not the terminal sialylation, assists

62 have evolved for the inhibition of in vitro fucosylation, but they are not applicable for in vivo us

63 (r) Fucosylation by alpha 1,2-L-FT of the galactosyl residue

64 SGL-1, including sialylation, sulfation, and fucosylation by alpha 1,3-fucosyltransferase(s) (FucT),

65 We unravel the mechanistic basis for fucosylation by AtFUT1 with a multipronged approach invo

66 abilized mucins post translationally through fucosylation by FUT8, as the knockdown of FUT8 resulted

67 acceptor substrate could facilitate the core fucosylation by FUT8.

68 Notch receptors are modified by O-fucosylation catalyzed by protein O-fucosyltransferase 1

69 ADAMTS9 showed that 9 of 12 TSRs with the O-fucosylation consensus sequence carried the Glucosebeta1

70 We investigated whether a lack of fucosylation consequent to loss of GDP-fucose synthesis

71 inner olfactory nerve layer, suggesting that fucosylation contributes to OB development.

72 In addition, the degree of anti-HPA-1a fucosylation correlated positively with the neonatal pla

73 nterfere with selectin-mediated recognition, fucosylation could negatively regulate siglec binding.

74 s, yielded a human cDNA that complements the fucosylation defect in the Lec13 cell line.

75 gnaling during development, and shows milder fucosylation defects than those observed in mice unable

76 We studied a mouse model of fucosylation deficiency (Fx-/- mice) and mice with the f

77 Fucosylation deficiency altered the composition of the f

78 onsequent to a molecular mechanism driven by fucosylation deficiency and/or HES1-loss.

79 In mice, fucosylation deficiency leads to colitis and adenocarcin

80 We show that fucosylation-deficient Lec13 Chinese hamster ovary cells

81 By contrast, fucosylation-deficient myeloid progenitors are insensiti

82 matopoietic stem cells deficient in cellular fucosylation display decreased frequency and defective r

83 n of three other highly conserved sites of O-fucosylation does not have detectable effects.

84 In the absence of fucosylation, dysplasia appeared and progressed to adeno

85 Glycoprotein fucosylation enables fringe-dependent modulation of sign

86 sylation in FX(-/-) mice identifies cellular fucosylation events as essential concomitants to fertili

87 We found that core fucosylation exerted a significant adverse effect on Fcg

88 The results of enforced fucosylation for 22 patients enrolled in the trial were

89 Consistent with very low endogenous fucosylation, forced fucosylation of intact WEHI-3 cells

90 ised by the presence of outer-arm alpha(1,3)-fucosylation (forming the Lewis[x] determinant), alpha(1

91 ation (FUT8) and downregulation of alpha-1,2 fucosylation (FUT1, FUT2) were identified as features of

92 Upregulation of core fucosylation (FUT8) and downregulation of alpha-1,2 fuco

93 of two enzymes involved in alpha-1,6 linked fucosylation, GDP-mannose 4, 6-dehydratase (Gmds) and to

94 , the presence of a bisecting GlcNAc or core-fucosylation had little effect on the affinity of Fc to

95 nositol (GPI) anchors and N-glycosylation, O-fucosylation has been recently reported in key sporozoit

96 Enhanced fucosylation has been suggested as a marker for serologi

97 Fucosylation has profound effects on the expression and

98 G1 monoclonal antibodies with reduced glycan fucosylation have been shown to improve antibody-depende

99 To study how changes in fucosylation impact embryonic development, we up-regulat

100 Core fucosylation impacted L1CAM cleavage and the ability of

101 The mur2 mutation eliminates xyloglucan fucosylation in all major plant organs, indicating that

102 911 sites of CFH, or a higher degree of core fucosylation in CFH compared to HPX, but we did not iden

103 t a valuable tool for studying the role of O-fucosylation in ECM synthesis and remodeling, and will b

104 on hypotheses regarding roles of xyloglucan fucosylation in facilitating xyloglucan-cellulose intera

105 Conditional control of fucosylation in FX(-/-) mice identifies cellular fucosyl

106 ncertain, as has a requirement for alpha(1,3)fucosylation in HEV L-selectin ligand activity.

107 nterparts, revealed a consistent increase in fucosylation in liver disease with significant site- and

108 (FUT8), the only enzyme catalyzing alpha1,6-fucosylation in mammals, has been observed in several ma

109 ed intestinal epithelial Fut2 expression and fucosylation in mice.

110 offer new insight into the role of alpha1,3 fucosylation in prostate cancer development.

111 ne a critical role for N-glycosylation and O-fucosylation in the biosynthesis of punctin-1.

112 vitro and identifies a requirement for Notch fucosylation in the expression of Notch ligand binding a

113 ew insights into the mechanism of xyloglucan fucosylation in the Golgi.

114 can be identified by the concentration of 2'-fucosylation in their milk.

115 ich we show are both substrates for N-linked fucosylation in zebrafish embryos.

116 h respect to sialylation, mannosylation, and fucosylation, in normal, pancreatitis, and cancer sera.

117 The target fucosylation inhibitor (1) was synthesized from readily

118 nd feasibility of a novel strategy, enforced fucosylation, intended to improve engraftment after dono

119 O-fucosylation is a common posttranslational modification

120 Furthermore, these results suggest O-fucosylation is a mechanism by which proteins involved i

121 he enzymes (fucosyltransferases) involved in fucosylation is a recurrent theme in modern glycoscience

122 Fucosylation is an important feature of protein N-glycos

123 Here we show that increased core fucosylation is associated with de-differentiation of pr

124 In particular, the level of core-fucosylation is critical to several Fc mediated biologic

125 O-Fucosylation is essential for receptor function, and elo

126 d CR/Nodal signaling assays, indicating that fucosylation is functionally important for CR to facilit

127 Together these findings indicate that O-fucosylation is functionally significant for secretion o

128 of N-glycans is reduced severely, protein O-fucosylation is generally unaffected.

129 O-Fucosylation is mediated by protein O-fucosyltransferase

130 Cell surface fucosylation is required for T3SS2-dependent killing, an

131 hich are conditionally deficient in cellular fucosylation, is consequent to loss of Notch-dependent s

132 Both fucosylation isoforms of sPSGL-1 bound to sP-selectin.

133 Disruption of intestinal fucosylation led to increased susceptibility to infectio

134 dhesion and spreading in vitro, as well as N-fucosylation level of the endometrium in pregnant mice.

135 ion of IgG1 monoclonal antibodies with lower fucosylation levels and thus improve the ADCC of these p

136 increased alpha1,2-, alpha1,3- and alpha1,6-fucosylation levels by up-regulating N-fucosyltransferas

137 he amino acids responsible for site-specific fucosylation map near the GDP-fucose-binding site.

138 broad perspective, these data suggest that O-fucosylation may be a widespread post-translational modi

139 t transfusion), indicating that the level of fucosylation may be antigen dependent and/or related to

140 he rs78060698 variant, through regulation of fucosylation may control intestinal host-microbial inter

141 Co-administration with MSCs or ex vivo fucosylation may enhance utility of CD34(+) cells in cel

142 ads us to propose an atypical water-mediated fucosylation mechanism facilitated by an H-bonded networ

143 Notch activity can be modulated by O-fucosylation (mediated by protein O-fucosyltransferase-1

144 The molecular mechanism, however, by which fucosylation mediates these processes remains largely el

145 the glycopeptide from normal SKP1 and from a fucosylation mutant, followed by matrix-assisted laser d

146 the extracellular domain of the receptor: O-fucosylation, O-glucosylation, and O-GlcNAcylation.

147 The fucosylation of a Notch1 EGF repeat fragment that occurs

148 nduced Notch signaling were reduced, and the fucosylation of a Notch1 fragment was also decreased.

149 wis x (17) was synthesized via the enzymatic fucosylation of a precursor displayed in the plate.

150 tizes domestic ruminants, have revealed core fucosylation of a type not previously observed in any eu

151 riantennary complex carbohydrate, as well as fucosylation of all types of chains.

152 We found markedly decreased levels of core fucosylation of anti-HPA-1a-specific IgG1 from FNAIT pat

153 It is possible that fucosylation of antithrombin may occur in vivo as a mean

154 mucin core 2 can proceed even after alpha1,3 fucosylation of beta1,6-linked LacNAc.

155 We found that inadequate alpha1-3 fucosylation of CB CD34(+) cells, particularly CD34(+)CD

156 These observations suggest that alpha1-3 fucosylation of CB cells might be a simple and effective

157 this defect appears related to low levels of fucosylation of cell surface molecules that are responsi

158 Inhibition of fucosylation of cell wall polysaccharides also affected

159 us, inhibition of Notch-Serrate binding by O-fucosylation of EGF12 might be needed in certain context

160 ven orally to mice, 2-fluorofucose inhibited fucosylation of endogenously produced antibodies, tumor

161 l step of Lex biosynthesis is the alpha(1,3)-fucosylation of GlcNAc in a terminal Galbeta(1-->4)GlcNA

162 (d) On C-3 or C-4 fucosylation of GlcNAc, both types 1 and 2 lost activity

163 ification is needed to confirm that enhanced fucosylation of HPX and CFH may serve as an indicator of

164 Core fucosylation of IgG is a "safety switch" reducing ADCC,

165 Nevertheless, forced fucosylation of intact cells did not significantly augme

166 -fucosynthase and fucoligase for direct core fucosylation of intact N-glycoproteins.

167 ith very low endogenous fucosylation, forced fucosylation of intact WEHI-3 cells or murine neutrophil

168 itionally, alpha1,2-, alpha1,3- and alpha1,6-fucosylation of integrin alphaVbeta3, a critical endomet

169 Fucosylation of intestinal epithelial cells, catalyzed b

170 rements requires sulfation, sialylation, and fucosylation of ligands.

171 These findings support ex vivo fucosylation of multipotent CD34(+) CB cells as a clinic

172 fucosyltransferase (FUT8) catalyzes the core fucosylation of N-glycans in the biosynthesis of glycopr

173 nt roles in directing the branching and core fucosylation of N-glycans in vivo.

174 Ic patients suggested that although terminal fucosylation of N-glycans is reduced severely, protein O

175 NAc2, suggesting that FUT8 can catalyze core fucosylation of N-glycans lacking an alpha1,3-arm GlcNAc

176 first report of in vitro FUT8-catalyzed core fucosylation of N-glycans lacking the alpha1,3-arm GlcNA

177 The core fucosylation of N-glycans on glycoproteins is catalyzed

178 Core fucosylation of N-glycoproteins plays a crucial role in

179 es of the young (MODY) would display altered fucosylation of N-linked glycans on plasma proteins and

180 Core fucosylation of N-linked oligosaccharides (GlcNAcbeta1,

181 in humans, requires sialylation and alpha1,3-fucosylation of neutrophils.

182 ose, or a factor that otherwise enhances the fucosylation of Notch and is required for optimal Notch

183 art through processes controlled by O-linked fucosylation of Notch receptors.

184 fects and mechanisms accounting for impaired fucosylation of selectin ligands and defective selectin

185 density, although a marked elevation in the fucosylation of Ser-linked glycans compared with Thr-lin

186 ceptor (TLR) ligands causes rapid alpha(1,2)-fucosylation of small intestine epithelial cells (IECs)

187 Previous studies suggest that O-fucosylation of the epidermal growth factor-like (EGF) r

188 The in vitro fucosylation of the nonglycosylated FVII EGF-1 was achie

189 This phenomenon is linked to changes in the fucosylation of the O chain, which was concomitant with

190 Fucosylation of the polylactosamine sequences on complex

191 Whereas in the double mutant core alpha1,3-fucosylation of the proximal N-acetylglucosamine was abo

192 ls revealed that galactosylation rather than fucosylation of the side chains is essential for mainten

193 ned to stipules and pollen grains leading to fucosylation of the walls of these cell types in the mur

194 fut2 gene-trap lines, we demonstrated that O-fucosylation of TSRs was essential for restricting epith

195 charides and glycoproteins demonstrated that fucosylation of xyloglucans and of N-linked glycans is f

196 In view of the influence of outer-arm fucosylation on carbohydrate recognition processes in ge

197 e split mutation introduced a new site for O-fucosylation on EGF repeat 14 of the Notch extracellular

198 However, sites of O-fucosylation on Notch that influence Notch activation ha

199 Previously, O-fucosylation on Ser or Thr mediated by the endoplasmic r

200 Either alpha1,3 fucosylation or 6-O-sulfation of the GlcNAc moiety reduc

201 We investigated whether O-fucosylation or Fringe-mediated elongation of O-fucose o

202 lass, presentation, opsonization density, Fc fucosylation, or mutation.

203 oteins with O-fucose; here we describe the O-fucosylation pathway in the nucleocytosol of a eukaryote

204 To elucidate the GnT I-independent core fucosylation pathway, we generated a stable HEK293S GnT

205 e responsible for the GnT I-independent core fucosylation pathway.

206 , implicating a clear GnT I-independent core fucosylation pathway.

207 Furthermore, the fucosylation pattern of PSGL-1 can affect its affinity f

208 In the context of a wild-type fucosylation phenotype, we find that the Notch ligands s

209 om several laboratories has indicated that O-fucosylation plays an important role in ligand mediated

210 dependent killing, and genetic inhibition of fucosylation prevents membrane insertion of the T3SS2 tr

211 ron, a component of this flora, restored the fucosylation program, whereas an isogenic strain carryin

212 rences are responsible for the site-specific fucosylation properties of each enzyme.

213 studies demonstrated that the site-specific fucosylation properties of these enzymes could be revers

214 ese two enzymes have distinct "site-specific fucosylation" properties.

215 GDP-fucose (GDP-Fuc), the precursor for all fucosylation reactions, in the blood stages of Plasmodiu

216 that the metabolite may be used for further fucosylation reactions.

217 o prepare activated L-fucose derivatives for fucosylation reactions.

218 We also observed that core fucosylation reduced the activity of GnT-IV toward the b

219 receptivity, as well as the regulation of N-fucosylation remains unclear.

220 and, commensurately, cell surface alpha(1,3)-fucosylation reveals that acceptor sialyllactosaminyl gl

221 In contrast, in the absence of fucosylation, sialylation did not adversely impact ADCC.

222 ular weight of the chains, and the levels of fucosylation, sialylation, and sulfation remain fairly c

223 Sialylation in the context of core fucosylation significantly decreased ADCC in a cell-base

224 For example, core fucosylation significantly decreases antibody-dependent

225 This O-fucosylation site did not meet the proposed consensus se

226 Unexpectedly, one additional O-fucosylation site was found in the disintegrin domain.

227 -like domain and the presence of a consensus fucosylation site within all EGF-CFC family members sugg

228 osylated form even for the residues near the fucosylation site.

229 We identified 7 O-fucosylation sites in the thrombospondin (TSP) type 1 re

230 ating that arabinosyl residues represent the fucosylation sites on these molecules.

231 has been successfully employed for assigning fucosylation, such strategies are often too cumbersome,

232 eceptor led to approximately 30% and 3% core fucosylation, suggesting that the level of core fucosyla

233 s in HA specificity, such as preferences for fucosylation, sulfation and sialylation at positions 2 (

234 hD) and platelet Ags frequently have reduced fucosylation that enhances their pathogenicity.

235 ,6-Trifluorofucose (1) is a new inhibitor of fucosylation that has been demonstrated to allow the pre

236 in mice conditionally deficient in cellular fucosylation that is attributable to a loss of Notch-dep

237 The relevance of gut fucosylation to human diseases also is discussed.

238 application toward the rapid verification of fucosylation types in a therapeutic protein (Rituximab).

239 Thus, the fucosylations unveil a proliferation-dependent switch in

240 was efficient in detection of N-glycan core fucosylation using lectin blotting and lectin ELISA assa

241 dition of fucose to the diet, which restored fucosylation via a salvage pathway.

242 that IL-22RA1 signaling promotes intestinal fucosylation via induction of the fucosyltransferase Fut

243 ryonic development, we up-regulated N-linked fucosylation via over-expression of a key GDP-Fucose tra

244 This increase in core fucosylation was associated with increased levels of two

245 ther enzyme) demonstrated that site-specific fucosylation was defined within a 40-amino acid segment

246 Unexpectedly, only small amounts of terminal fucosylation was found in diantennary complex-type N-gly

247 To test the functional consequences of Ab fucosylation, we produced V-gene-matched recombinant ant

248 ecifically, whereas alpha2,6-sialylation and fucosylation were not.

249 st to the FNAIT patients, no changes in core fucosylation were observed for anti-HLA antibodies in re

250 , including N-glycosylation, sialylation and fucosylation, were observed between early and late time

251 n a receptive endometrium by up-regulating N-fucosylation, which is a potential useful biomarker to e

252 these FX-deficient cells exhibited defective fucosylation, which is required for Notch signaling.

253 fucosylated N-glycans rapidly verifies core fucosylation while its absence signifies antennae fucosy

254 iabetic CD34(+) cells manipulated by ex vivo fucosylation with ASC-101.

255 937 lymphoma cells, the glycosides decreased fucosylation without affecting sialylation.

256 tions to GlyCAM-1, together with appropriate fucosylation, yields enhanced rolling ligands for both p