ライフサイエンスコーパス: fucosyltransferase

1 syltransferase 2 is in fact a TSR-specific O-fucosyltransferase.

2 sferase activity with minimal effects on the fucosyltransferase.

3 genes confirm that each encodes an alpha(1,2)fucosyltransferase.

4 compared with those generated with exogenous fucosyltransferase.

5 of a fucose residue in GDP-fucose and a milk fucosyltransferase.

6 y the PgtA galactosyltransferase but not the fucosyltransferase.

7 surface expression of Notch and a protein O-fucosyltransferase.

8 at is defective for sialylation and alpha1,3-fucosyltransferase.

9 ha, a key regulator of expression of various fucosyltransferases.

10 omosome 19q13.3 that encodes three alpha(1,2)fucosyltransferases.

11 organs, indicating that Arabidopsis thaliana fucosyltransferase 1 (AtFUT1) accounts for all of the xy

12 ana, a glycosyl transferase family 37 (GT37) fucosyltransferase 1 (AtFUT1) catalyzes the regiospecifi

13 ed in LewisX biosynthesis, naming it chicken fucosyltransferase 1 (CFT1).

14 ed the subcellular localization of protein O-fucosyltransferase 1 (O-FucT-1), which is responsible fo

15 We found that in Drosophila, Protein O-fucosyltransferase 1 (OFUT1), an enzyme that glycosylate

16 ke repeats that may be modified by protein O-fucosyltransferase 1 (Pofut1), an essential component of

17 ith mutations in keratin 5 (KRT5), protein O-fucosyltransferase 1 (POFUT1), or protein O-glucosyltran

18 ied by O-fucosylation catalyzed by protein O-fucosyltransferase 1 (Pofut1).

19 rated that an enzyme distinct from protein O-fucosyltransferase 1 adds O-fucose to TSRs.

20 O-linked glycosylation mediated by protein O-fucosyltransferase 1 and Fringe.

21 we show that mouse embryos lacking protein O-fucosyltransferase 1 die at midgestation with severe def

22 Here we show that the protein O-fucosyltransferase 1 gene (Pofut1), which encodes a glyc

23 othesis and show the following: 1) protein O-fucosyltransferase 1 is indeed the enzyme that adds O-fu

24 the transfer of fucose to Notch by protein O-fucosyltransferase 1 is necessary for Fringe to function

25 A known homologue of O-fucosyltransferase 1 is putative protein O-fucosyltransf

26 Protein O-fucosyltransferase 1 is therefore an essential core memb

27 lacking both maternal and zygotic protein O-fucosyltransferase 1, a cell-autonomous and essential co

28 ss these issues, the gene encoding protein O-fucosyltransferase 1, an enzyme required for Notch ligan

29 O-Fucosylation is mediated by protein O-fucosyltransferase 1, and down-regulation of this enzyme

30 Like O-fucosyltransferase 1, O-fucosyltransferase 2 is conserve

31 However, embryos null for protein O-fucosyltransferase 1, the enzyme that adds O-fucose to E

32 n, a reaction that is catalyzed by protein O-fucosyltransferase-1 (Pofut1).

33 ted by O-fucosylation (mediated by protein O-fucosyltransferase-1) and Fringe, a beta1,3-N-acetylgluc

34 Another alpha1,3-fucosyltransferase gene, fucosyltransferase 10 (Fut10), is expressed in the ventr

35 n of innate lymphoid cells and expression of fucosyltransferase 2 (Fut2) by IL-22-stimulated IECs.

36 d group antigens, which are expressed by the fucosyltransferase 2 (FUT2) gene.

37 Genetic variants of fucosyltransferase 2 (FUT2) have been identified in geno

38 ncentration and the 461G-->A polymorphism of fucosyltransferase 2 (FUT2), a gene associated with susc

39 of intestinal epithelial cells, catalyzed by fucosyltransferase 2 (Fut2), is a major glycosylation me

40 bospondin type 1 repeats (TSRs) by protein O-fucosyltransferase 2 (POFUT2) and is elongated with gluc

41 plasmic reticulum-localized enzyme protein-O-fucosyltransferase 2 (POFUT2) was described for TSRs of

42 When the expression of protein O-fucosyltransferase 2 (POFUT2), the enzyme that transfers

43 f thrombospondin type 1 repeats by protein O-fucosyltransferase 2 (POFUT2).

44 rmore, we expressed recombinant Drosophila O-fucosyltransferase 2 and showed that it O-fucosylates TS

45 Like O-fucosyltransferase 1, O-fucosyltransferase 2 is conserved from Caenorhabditis el

46 These results demonstrate that O-fucosyltransferase 2 is in fact a TSR-specific O-fucosyl

47 We also found that O-fucosyltransferase 2 is predominantly localized in the e

48 e show that RNAi-mediated reduction of the O-fucosyltransferase 2 message significantly decreased TSR

49 Although O-fucosyltransferase 2 was assumed to be another protein O

50 The cDNA sequence encoding O-fucosyltransferase 2 was originally identified during a

51 O-fucosyltransferase 1 is putative protein O-fucosyltransferase 2.

52 ction mechanism similar to that of protein-O-fucosyltransferase 2.

53 this study, we verified that mouse protein O-fucosyltransferase-2 (POFUT2) specifically adds O-fucose

54 DM4, IDDM5, IDDM6, IDDM8, and IDDM10 and the fucosyltransferase-2 locus for linkage in sib pairs with

55 of glycosyltransferase expression identified fucosyltransferase 3 (Fut3) as the key enzyme driving sL

56 trate that hematopoietic progenitors lacking fucosyltransferase 4 and 7 do not express functional PSG

57 Expression of fucosyltransferase 6 resulted in marked increases in lev

58 overcome this deficiency, we expressed human fucosyltransferase 6 using modified mRNA.

59 nds contribute to metastasis using alpha(1,3)fucosyltransferase 7 (Fuc-TVII(-/-))-deficient mice.

60 e examined the transcriptional regulation of fucosyltransferase 7 (FUT7), an enzyme crucial for gener

61 f N-glycans on glycoproteins is catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and is in

62 The up-regulation of fucosyltransferase 8 (FUT8), the only enzyme catalyzing

63 6-dehydratase (Gmds) and to a lesser extent fucosyltransferase 8 (Fut8).

64 n this study, we examined mice deficient for fucosyltransferase 9 (Fut9), which is thought to synthes

65 -strand mispairing in a poly(C) tract of the fucosyltransferase A (fucT1) gene.

66 nd murine neutrophils expressed low alpha1-3-fucosyltransferase activities.

67 cDNA, significantly raising their levels of fucosyltransferase activity and surface sLe(x/a).

68 and a nonradioactive, fluorescence assay for fucosyltransferase activity have been developed.

69 (AtFUT1) accounts for all of the xyloglucan fucosyltransferase activity in Arabidopsis.

70 ssage significantly decreased TSR-specific O-fucosyltransferase activity in Drosophila S2 cells.

71 thesis of each occurs at different levels of fucosyltransferase activity in intact cells.

72 ed sLe(x/a) is because of decreased alpha3/4-fucosyltransferase activity in MARY-X.

73 yzed by FT85, a 768-amino acid protein whose fucosyltransferase activity maps to the C-terminal half

74 In addition, the alpha1,2-fucosyltransferase activity of GST-WbsJ was found to be

75 alylated structures by high-level alpha(1,2)-fucosyltransferase activity reduces monocyte adherence a

76 In vitro CFT1 fucosyltransferase activity utilizes LacNAc > 3'sialyl-L

77 that Fut10 is involved in a unique alpha1,3-fucosyltransferase activity with stringent substrate spe

78 ialyl Lewis x, sialyl Lewis a, alpha(1,3/1,4)fucosyltransferase activity, and FUT3 transcript, but an

79 ilitate folding of Notch did not require its fucosyltransferase activity.

80 exhibits beta-galactosyltransferase but not fucosyltransferase activity.

81 CHO cells also lack alpha1 3 fucosyltransferase activity.

82 d a protein with both alpha1,3- and alpha1,4-fucosyltransferase activity.

83 genic mice by expressing a human alpha 1,3/4-fucosyltransferase (alpha 1,3/4-FT; EC 2.4.1.65) along t

84 g GDP-fucose:N-acetylglucosaminyl alpha(1,3) fucosyltransferase (alpha(1,3)-Fuc-T) activity was recen

85 in-of-function mutant expresses an alpha(1,3)fucosyltransferase (alpha(1,3)Fuc-T) activity that gener

86 sferase [alpha(2,3)ST], or Galbeta 2-alpha-L-fucosyltransferase [alpha(1,2)FT; EC 2.4.1.691, etc.

87 6 melanoma was transfected with the alpha1,2-fucosyltransferase (alpha1,2FT) cDNA.

88 n H-type GDPFuc:beta-D-galactoside alpha1, 2-fucosyltransferase (alpha1,2FT) was stably transfected i

89 antial redundancy in the mammalian alpha-1,3-fucosyltransferase and alpha-1,2-fucosyltransferase gene

90 ts, enzymatic transfers with a milk alpha1,3-fucosyltransferase and an alpha2,3-sialyltransferase (ST

91 eting expression of the endogenous alpha-1,3-fucosyltransferase and beta-1,2-xylosyltransferase genes

92 mportant breakthroughs in the development of fucosyltransferase and fucosidase inhibitors.

93 ibitory activities correlated inversely with fucosyltransferase and sialyltransferase activity based

94 scale) of an endoplasmic reticulum-resident fucosyltransferase and two potential anticancer protein

95 hat the common motif shared by both alpha1,2-fucosyltransferases and alpha1,6-fucosyltransferases hav

96 ences in the relative activities of alpha1,3-fucosyltransferases and alpha2,3-sialyltransferases in t

97 Fucosyltransferases and fucosidases, the main enzymes in

98 involved in glycan modifications, including fucosyltransferases and sialyltransferases, during infla

99 C79a/Ig-alpha, C79b/Ig-beta, and Fut3/alpha-fucosyltransferase); and (ii). variants of bacteriophage

100 yltransferase1 (GALT1), Arabidopsis alpha1,4-fucosyltransferase, and Rattus norvegicus alpha2,6-sialy

101 lysis predicts that these family members are fucosyltransferases, and we first hypothesized that some

102 ate that this cDNA and its cognate alpha(1,3)fucosyltransferase are expressed in endothelial cells li

103 Fucalpha(1-->2)Galbeta moieties and cognate fucosyltransferases are also expressed by epithelial cel

104 These data demonstrate that fucosyltransferases are important in the pathogenesis of

105 e various studies, the specificities of many fucosyltransferases are still unknown, so new approaches

106 human FucT using a structure of a bacterial fucosyltransferase as a template demonstrated that the a

107 yloglucan because of a point mutation in the fucosyltransferase AtFUT1.

108 acid similarity with the xyloglucan-specific fucosyltransferase AtFUT1.

109 requirement for E-selectin ligands, alpha1,3 fucosyltransferases, beta1 and alphaVbeta3 integrins, an

110 ls, however, requires expression of alpha1-3-fucosyltransferases but not PSGL-1.

111 whether high-level expression of alpha(1,2)-fucosyltransferase by porcine endothelium would reduce h

112 ficant differences in expression of alpha1,3-fucosyltransferases, C2GnT (Core2 transferase), or P-sel

113 Human alpha-1,3-fucosyltransferase catalyzes the transfer of the L-fucos

114 -1 cDNA is coexpressed with an alpha 1,3/1,4 fucosyltransferase cDNA in COS cells, a functional prote

115 A novel fucosyltransferase (cFTase) activity has been enriched o

116 gain-of-function experiments where all three fucosyltransferases conferred E-selectin-mediated rollin

117 Fut7 encodes an alpha1,3-fucosyltransferase critical for biosynthesis of glycan l

118 Although overexpression of fucosyltransferase-defective Pofut1 R245A in Pofut1-/- c

119 Furthermore, studies with fucosyltransferase-deficient mice suggest that sialylate

120 glucosaminyltransferases IV and V and alpha6-fucosyltransferase during normal pregnancy.

121 -fucose:beta(1-->4)-D-galactosyl-R 2-alpha-L-fucosyltransferase enzymes (EC 2.4.1.69) responsible for

122 leles of the Pofut1 gene, which encodes an O-fucosyltransferase essential for Notch-ligand binding.

123 egrins and targeted deletion of an alpha(1,3)fucosyltransferase essential for selectin ligand synthes

124 re 1 beta 3-galactosyltransferase and alpha2-fucosyltransferase exhibit unique peptide/glycopeptide s

125 helial cell line transfected with alpha(1,2)-fucosyltransferase, expressing reduced surface expressio

126 y factors resulted in the down-regulation of fucosyltransferase expression, reflected by altered glyc

127 yltransferase (ST) activity and low alpha1,2-fucosyltransferase (FT) activities were detected from du

128 Here we report the discovery of a unique fucosyltransferase (FT) in Caenorhabditis elegans.

129 ned blood group H gene-specified alpha 1,2-L-fucosyltransferase (FT) toward a variety of sulfated, si

130 We demonstrate that alpha1,3 fucosyltransferases (FT) 3, 6, and 7 are markedly elevat

131 redominant HUVEC sialyltransferases (ST) and fucosyltransferases (FT), key enzymes in sLe(x) and Le(x

132 A 60-kilodalton fucosyltransferase (FTase) that adds this residue was pu

133 ique specificities of the cloned alpha 1,3-L-fucosyltransferases (FTs), FT III (Lewis type), FT IV (m

134 or murine neutrophils by exogenous alpha1-3-fucosyltransferase FTVI and GDP-fucose created many new

135 trate here that mice deficient in alpha(1,3) fucosyltransferase Fuc-TVII exhibit a leukocyte adhesion

136 To determine how the alpha(1,3)fucosyltransferases Fuc-TIV and Fuc-TVII, and the select

137 en in wild-type mice; mice lacking alpha 1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice lackin

138 e sialyltransferase ST3Gal-III compared with fucosyltransferases Fuc-TIV/VII in the synthesis of the

139 have been characterized against recombinant fucosyltransferase (Fuc-T) V using ESI-MS/MRM.

140 co-expressed with both C2GnT and an alpha1 3 fucosyltransferase (Fuc-TIII, Fuc-TIV, or Fuc-TVII).

141 s deficient in the expression of alpha-(1, 3)fucosyltransferase (Fuc-TVII), an enzyme known to be req

142 or both PSGL-1 and its modifying alpha-(1,3) fucosyltransferase, Fuc-TVII, allowed binding and infect

143 the catalytic domain of two human alpha1,3/4-fucosyltransferases (fucosyltransferases (FucTs) III and

144 Induction of the alpha1,3-fucosyltransferase FucT-VII in T lymphocytes is crucial

145 The alpha(1,3)-fucosyltransferase FucT-VII is essential for the biosynt

146 r laboratories indicated that the alpha(1,3)-fucosyltransferase FucT-VII regulates the synthesis of E

147 ed prominently but incompletely by alpha(1,3)fucosyltransferase FucT-VII-dependent fucosylation.

148 E-selectins and that the leukocyte alpha(1,3)fucosyltransferases FucT IV and FucT VII do not provide

149 tin ligand biosynthesis include the alpha1,3-fucosyltransferases FucT-VII and FucT-IV, one or more si

150 In plants, alpha1,3-fucosyltransferase (FucT) catalyzes the transfer of fuco

151 We hypothesized that fucosyltransferase (FucT) enzymes were important pathoph

152 his deficiency results from reduced alpha1,3-fucosyltransferase (FucT) expression and activity in the

153 A GDP-fucose:GM1 alpha1-->2 fucosyltransferase (FucT) is induced during early stages

154 absent in mice doubly deficient in alpha1,3-fucosyltransferase (FucT)-IV and FucT-VII.

155 in ligands must be fucosylated by alpha(1,3)-fucosyltransferase (FucT)-IV or FucT-VII as rolling is a

156 Human alpha1,3/4-fucosyltransferase (FucT)-V catalyzes primarily the synt

157 alpha-(1,3)-Fucosyltransferase (FucT)-VII-deficient OT-I cells displ

158 reases in the two other leukocyte-associated fucosyltransferases (FucT-IV and VI).

159 The activity of leukocyte alpha1,3-fucosyltransferases (FucT-IV or FucT-VII) is an essentia

160 The alpha1,3-fucosyltransferase, FucT-VII, is crucial for the formati

161 ion of the two principal leukocyte alpha 1,3 fucosyltransferases, FucT-IV and FucT-VII, in a panel of

162 Human alpha1,3 fucosyltransferases (FucTs) contain four highly conserve

163 acid sequence alignment of human alpha1, 3/4-fucosyltransferases (FucTs) demonstrates that three high

164 y of domain swap mutants of human alpha1,3/4-fucosyltransferases (FucTs) III and V has been carried o

165 of two human alpha1,3/4-fucosyltransferases (fucosyltransferases (FucTs) III and V), and to identify

166 transfer of two of these (the core alpha1,3-fucosyltransferase FUT-1 and the core alpha1,6-fucosyltr

167 cosyltransferase FUT-1 and the core alpha1,6-fucosyltransferase FUT-8) were previously characterized.

168 e surface glycans by inhibition of alpha(1,3)fucosyltransferase (FUT) gene expression is an attractiv

169 a GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT) in an antisense orientation in

170 The xyloglucan-specific alpha2-fucosyltransferase FUT1 catalyzes the transfer of fucose

171 gars in the OB is regulated by the alpha(1-2)fucosyltransferase FUT1.

172 ha1,6-fucosylation levels by up-regulating N-fucosyltransferases FUT1, FUT4 and FUT8 expression, resp

173 intestinal fucosylation via induction of the fucosyltransferase Fut2.

174 phenotype due to knockout of their alpha1-2 fucosyltransferase (Fut2) gene.

175 contributions of all three myeloid alpha1,3-fucosyltransferases (FUT4, FUT7, and FUT9) to selectin-l

176 The mammalian alpha1,6-fucosyltransferase (FUT8) catalyzes the core fucosylatio

177 r the inability of the biosynthetic alpha1,6-fucosyltransferase (FUT8) to directly fucosylate full-si

178 In this case, the third alpha1,3-fucosyltransferase FUT9 played an important role because

179 XyG fucosyltransferase (FUTase), encoded by the Arabidopsis

180 hat AtFUT4 and AtFUT6 genes encode alpha(1,2)fucosyltransferases (FUTs) for AGPs.

181 n was homozygous recessive for the alpha(1,2)fucosyltransferase gene (FUT2) in the ABH histo-blood gr

182 infection, and expression of the alpha(1,2) fucosyltransferase gene (FUT2) responsible for the secre

183 from transgenic pigs expressing the alpha1,2 fucosyltransferase gene (H-transferase or HT) gene into

184 located between the secretor type alpha(1,2)-fucosyltransferase gene cluster (FUT1-FUT2-FUT2P) and th

185 lon, a 3.3-kb FUT2 mRNA represents the major fucosyltransferase gene expressed.

186 n alpha-1,3-fucosyltransferase and alpha-1,2-fucosyltransferase gene families.

187 cloned and expressed the chicken alpha(1,3)-fucosyltransferase gene involved in LewisX biosynthesis,

188 othelium by transfection with the alpha(1,2)-fucosyltransferase gene reduced susceptibility to human

189 transferase genes and demonstrate a role for fucosyltransferase gene regulation in the developmental

190 Another alpha1,3-fucosyltransferase gene, fucosyltransferase 10 (Fut10),

191 es directed at the human Lewis alpha(1,3/1,4)fucosyltransferase gene, FUT3.

192 nce of the repeats, including the alpha(1-2) fucosyltransferase gene, necessary for the synthesis of

193 etween nonmammalian and mammalian alpha(1,3)-fucosyltransferase genes and demonstrate a role for fuco

194 es from the open reading frames of the mouse fucosyltransferase genes corresponding to human FUT1, FU

195 hese and other results suggest that multiple fucosyltransferase genes in C. elegans may encode enzyme

196 motif which may be of use in identifying new fucosyltransferase genes.

197 ing HAR is the expression of human alpha1, 2-fucosyltransferase (H-transferase, HT).

198 ndothelial cell transfection with alpha(1,2)-fucosyltransferase has been shown to reduce terminal sia

199 th alpha1,2-fucosyltransferases and alpha1,6-fucosyltransferases have similar functions.

200 Expression of human alpha1,2-fucosyltransferase (HT) in the donor cell or tissue prot

201 dentification of the gene encoding protein O-fucosyltransferase I now makes possible mutational strat

202 on of amino acids found in human alpha1, 3/4-fucosyltransferase III (FucT III) conferred a significan

203 ted from CHO cells cotransfected with either fucosyltransferase III (sPSGL-1/Fuc-TIII) or fucosyltran

204 cells were stably transfected with alpha1, 3-fucosyltransferase III to express sialyl Lewis X structu

205 t that the AtFUT family is likely to include fucosyltransferases important for the synthesis of wall

206 lly identified during a data base search for fucosyltransferases in Drosophila.

207 s, we have investigated the role of alpha1,3-fucosyltransferases in generating E-selectin ligands, an

208 quences and confirm the primacy of alpha(1,3)fucosyltransferases in the synthesis of selectin ligands

209 ting E-selectin ligand-synthesizing alpha1,3 fucosyltransferases in transgenic adenoma of mouse prost

210 Transfection with fucosyltransferase induces expression of functional L-se

211 binding or for localization of the alpha1,2-fucosyltransferase involved in O-antigen biosynthesis.

212 loitation of the specificity of the enzymes (fucosyltransferases) involved in fucosylation is a recur

213 otein ligand-1 (PSGL-1) modified by alpha1,3-fucosyltransferase is the principal selectin ligand on s

214 t evidence that FUT8, the mammalian alpha1,6-fucosyltransferase, is the sole enzyme responsible for t

215 cosylated sLe(x) receptors, and two enzymes, fucosyltransferase IV (FucT-IV) and VII (FucT-VII), are

216 The alpha1,3-fucosyltransferase IV (FucTIV) encoded by its gene (FUTI

217 d alpha-2,3-sialyltransferases and alpha-1,3-fucosyltransferases IV and VI were determined, and the r

218 d alpha-2,3-sialyltransferases and alpha-1,3-fucosyltransferases IV and VI.

219 Mice deficient in alpha1,3 fucosyltransferases IV and VII (FTIV/VII) cannot synthes

220 2 beta1,6-N-acetylglucosaminyltransferase or fucosyltransferases IV/VII were impaired for engraftment

221 Fucosyltransferase-IV and -VII double knockout (FtDKO) m

222 hat express the Fut9 gene encoding alpha(1,3)fucosyltransferase IX (alpha(1,3) Fuc-TIX).

223 We also stably expressed human alpha1,3-fucosyltransferase IX in the L8-GalNAcT cells to establi

224 g and characterization of a murine alpha(1,3)fucosyltransferase locus whose expression pattern correl

225 fferently to conformational changes, and the fucosyltransferases lost less activity than the sialyltr

226 fucosylated glycoconjugates and an alpha1, 2-fucosyltransferase messenger RNA in the small-intestinal

227 veal that FUT-6, another C. elegans alpha1,3-fucosyltransferase, modifies nematode glycan cores, spec

228 ferase 2 was assumed to be another protein O-fucosyltransferase, no biochemical characterization exis

229 In contrast to alpha1,6-fucosyltransferases, none of the mutants of WbsJ within

230 th factor-like repeats, GDP-fucose protein O-fucosyltransferase (O-FucT-1), was purified previously f

231 nalysis reveals that, unlike all other known fucosyltransferases, O-FucT-1 is a soluble protein that

232 er of the large GT-B fold family, like other fucosyltransferases of known structures, it contains a v

233 and its ligands are modified by a protein O-fucosyltransferase (OFUT1) that attaches fucose to a Ser

234 is presumed to require one or more alpha(1,3)fucosyltransferases, operating upon common 3'-sialylated

235 Together with the fucosyltransferase POFUT2, B3GLCT adds Glucosebeta1-3Fuc

236 Using an alpha-1,3-fucosyltransferase preparation and enzymatic conditions

237 cosylated FVII EGF-1 was achieved by using O-fucosyltransferase purified from Chinese hamster ovary c

238 state of GDP-fucose complexed with Mn(II) in fucosyltransferase reactions.

239 ly and indicates that sialyltransferases and fucosyltransferases recognize N-acetyllactosamine in a d

240 ndothelial cell transfection with alpha(1,2)-fucosyltransferase reduced terminal sialic acid expressi

241 l mediator of Notch receptors, and Pofut1, a fucosyltransferase required for the activity of Notch re

242 scherichia coli O128:B12 encodes an alpha1,2-fucosyltransferase responsible for adding a fucose onto

243 obiose unit of these N-glycans, but only the fucosyltransferases responsible for transfer of two of t

244 on, sulfation, and fucosylation by alpha 1,3-fucosyltransferase(s) (FucT), are required for functiona

245 The identity of the alpha(1,3)fucosyltransferase(s) expressed in cells that bear L-sel

246 The identity of the alpha(1,3)fucosyltransferase(s) required for their expression has

247 ster ovary (CHO) cells expressing PSGL-1 and fucosyltransferase show a dramatic increase in binding t

248 tivity of the sialyltransferases whereas the fucosyltransferases showed some activity, albeit very lo

249 were stably transfected with alpha(1,3/1,4) fucosyltransferase-specific cDNA (B16F10ft), allowing th

250 studies and structure comparison with other fucosyltransferases suggest that FUT1 uses a SN2-like re

251 rt provides the first evidence that a single fucosyltransferase, termed FucT-VII, controls the synthe

252 function beta3-galactosyltransferase/alpha2-fucosyltransferase that contributes the 2nd and 3rd suga

253 FUT2 encodes the alpha(1,2) fucosyltransferase that determines blood group secretor

254 Pea microsomes contain an alpha-fucosyltransferase that incorporates fucose from GDP-fuc

255 dy binds fucosyltransferase1, an alpha-(1,2)-fucosyltransferase that synthesizes H-type structures on

256 glycoprotein ligand-1 (PSGL-1) and alpha1-3-fucosyltransferases that construct the glycan determinan

257 xpression of Fut genes that encode alpha-1,3-fucosyltransferases, the enzymes that generate the Le(x)

258 ochemically identified to encode an alpha1,2-fucosyltransferase through radioactivity assays, as well

259 d cells of GDP-fucose, the substrate used by fucosyltransferases to incorporate fucose into protein a

260 The alpha(1,3)-fucosyltransferases, types IV and VII (FUT4 and FUT7, re

261 alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of

262 cceptor substrate specificity into alpha1, 3-fucosyltransferase V (FucT V), which, under the same ass

263 as the donor substrate for recombinant human fucosyltransferase V, and GDP-d-[3H]arabinosep serves as

264 S) has been developed and demonstrated using fucosyltransferase V.

265 hosphate (GDP) fucose and exogenous alpha1-3 fucosyltransferase VI increased cell-surface sLe(x) dete

266 eated ex vivo for 30 minutes with the enzyme fucosyltransferase-VI and guanosine diphosphate fucose t

267 The selectin pathway recruiter, alpha-1,3-fucosyltransferase-VI enzyme, significantly increased Tr

268 Fucosyltransferase VII (Fuc-T VII) and core 2 beta-1,6-N

269 We show that alpha(1, 3)-fucosyltransferase VII (FucT-VII) and alpha(2, 3)-sialyl

270 trophils also expressed transcripts encoding fucosyltransferase VII (FucT-VII) and Core2GlcNAcT-I, wh

271 pressed equivalently high levels of alpha1,3-fucosyltransferase VII (FucT-VII) as wild-type Th1 cells

272 hese ligands is the expression of alpha(1,3)-fucosyltransferase VII (FucT-VII), a FucT essential for

273 have determined the role of tissue-specific fucosyltransferase VII (FucT-VII), an enzyme necessary f

274 (Stat4)-independent increase in Th1 cells of fucosyltransferase VII (FucT-VII).

275 ed because of targeted mutation of alpha-1,3-fucosyltransferase VII (Fut7).

276 fucosyltransferase III (sPSGL-1/Fuc-TIII) or fucosyltransferase VII (sPSGL-1/Fuc-TVII).

277 tment (resulting from targeted disruption of fucosyltransferase VII [FTVII]), and the absence of matu

278 In this study, fucosyltransferase VII cDNA (Fuc-TVII) transfection of t

279 Th1 cells also expressed more fucosyltransferase VII mRNA than naive or Th2 cells.

280 we transduced Jurkat (JK) T cells expressing fucosyltransferase VII with a chimeric chemokine recepto

281 ese hamster ovary cells, along with CD34 and fucosyltransferase VII, results in ligand activity, as d

282 e cell-surface receptor, PSGL-1, mediated by fucosyltransferase VII, serves as a mechanism for regula

283 arting JK lines, the resulting cell line (JK fucosyltransferase VII-CCR6) migrated 6-fold better to C

284 cotransfected with cDNAs encoding alpha (1,3)fucosyltransferase-VII (FucT-VII) and PSGL-1 rolled on L

285 ontrast to cells cotransfected with alpha1-3 fucosyltransferase-VII (FucT-VII) plus PSGL-1, K562 cell

286 (+) cells express both E-selectin ligand and fucosyltransferase-VII (FucT-VII).

287 -selectin, correlated with elevated alpha1,3-fucosyltransferase-VII messenger RNA levels, but selecti

288 ferases alpha2,3-sialyltransferase, alpha1,3-fucosyltransferase-VII, and core 2 beta1,6 N-acetylgluco

289 ific glycosyltransferases including alpha1,3-fucosyltransferase-VII, core 2 beta1-6-N-glucosaminyltra

290 ated, and negligibly inhibitory, whereas the fucosyltransferase was active toward small substrates.

291 A GDP-fucose:polypeptide fucosyltransferase was purified 5000-fold to homogeneity

292 atode N-glycan core in vitro using all three fucosyltransferases was performed, and the nature of the

293 with another recently characterized alpha1,2-fucosyltransferase (WbsJ) of E. coli O128:B12 indicates

294 In contrast to other alpha1,2-fucosyltransferases, WbwK does not display activity towa

295 es of FKP and a Helicobacter pylori alpha1,3 fucosyltransferase, we prepared a library of Le(x) trisa

296 sferase activity and a decrease in alpha(1,3)fucosyltransferases when these cells differentiate towar

297 Thus, FucT III is an alpha1,4-fucosyltransferase, whereas FucT V is an alpha1,3-fucosy

298 in CHO LEC11 cells with an active alpha(1,3)-fucosyltransferase, which makes possible the biosynthesi

299 yltransferase, whereas FucT V is an alpha1,3-fucosyltransferase with disaccharide substrates.