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1 r the Japanese pufferfish Takifugu rubripes (fugu).
2 roximity to the Herc2 gene in both mouse and Fugu.
3 of more distant organisms such as human and fugu.
4 genome that are conserved between human and Fugu.
5 o human chromosome 20 are also duplicated in Fugu.
6 ng to their endogenous expression pattern in fugu.
7 size of the intergenic region is smaller in Fugu.
8 esence of RAG transcripts in kidney of adult Fugu.
9 eral mesoderm may account for pelvis loss in fugu.
10 among human, mouse, chicken, zebrafish, and Fugu.
11 s, with a portion of a third Hoxa cluster in fugu.
12 logs such as human and chicken, or human and fugu.
13 eptors in both zebrafish and the pufferfish, Fugu.
14 and those being conserved between human and fugu.
15 crosatellite occurs every 1.876 kb of DNA in Fugu, 11.55% of the microsatellites are detected in open
16 f proteins found in individual datasets) and Fugu (14%) secretomes based on analysis of their nearly
17 divergence, we have cloned and sequenced the Fugu 5-HT type 1 receptor genes by Polymerase Chain Reac
18 hibits 58% sequence identity to the putative Fugu ACS and approximately 30% sequence identity to plan
21 We have identified four Hox complexes in Fugu and found an unprecedented degree of variation when
22 nservation of intron/exon boundaries between Fugu and human CFTR and revealed extensive homology betw
26 , gene orders are not well conserved between Fugu and human, with only very short sections of two to
29 efore hypothesized that by juxtaposing human/Fugu and human/mouse conservation patterns we can define
30 tion of regions of conserved synteny between Fugu and humans would greatly accelerate the mapping and
32 he syntenic relationship between mammals and Fugu and looked at the efficacy of ORF prediction from s
36 we cloned putative regulatory regions of the fugu and medaka Hoxa2(a) and -(b) genes and assayed thei
37 e test showed that zebrafish per1a/per1b and fugu and medaka per2a/per2b have asymmetric evolutionary
38 nked LMP7 pseudogene, indicating that within Fugu and potentially other teleosts, there has been an a
48 f human, chimpanzee, mouse, rat, pufferfish (Fugu) and zebrafish demonstrates that these six genes sh
49 ative assignments with tetraodon, zebrafish, fugu, and medaka resulting in assignments of homology fo
50 a and per1b, one per2, and one per3; medaka, fugu, and tetraodon each have two per2 genes, per2a and
51 ud outgrowth and initiation fail to occur in fugu, and that pelvis loss is associated with altered ex
53 genomic sequences spanning the human and the Fugu APP genes were analysed with a set of exon and gene
54 dicted human proteins have a strong match to Fugu, approximately a quarter of the human proteins had
55 the frequently occurring microsatellites in Fugu are known to code in other species for regions in p
58 To date, this syntenic association of the Fugu C4 and other MHC class III region genes has not bee
64 man chromosome 9q22, and lie adjacent to the Fugu C9/DOC-2 locus, indicating the boundary between two
66 wever, the immediate 5' regions of human and Fugu CFTR are highly divergent with few conserved sequen
71 rfeit genes in higher vertebrates, but these Fugu CpG islands are similar to the nonclassical islands
78 C2 genes have been identified in mouse, rat, Fugu, Drosophila, and in the yeast Schizosaccharomyces p
80 Genome-scale comparisons of noncoding human/Fugu evolutionary conserved elements (ECRs) and their hu
87 hout the protein coding region, although the Fugu gene is five times smaller than the mouse gene.
88 show that these are highly reliable for the Fugu gene with lower false positive and false negative r
89 u genes; in general, levels of compaction of Fugu genes are consistent with the isochore locations of
98 th those for a number of previously reported Fugu genes; in general, levels of compaction of Fugu gen
99 ncoding ECRs without the assistance of human-Fugu genome alignments and provides a very efficient fil
101 om data set covers nearly 25 Mb (>6%) of the Fugu genome and forms the basis of a series of whole gen
102 inked to Surfeit genes in two regions of the Fugu genome and have mapped and ordered their human homo
104 rmatic searches of Zebrafish, Tetraodon, and Fugu genome and other teleost expressed sequence tag dat
105 garding gene density and distribution in the Fugu genome and the similarity between Fugu and mammalia
106 , some of the genes that are adjacent in the Fugu genome are separated by at least 2-4 Mb in the huma
108 Analysis of the EST data compared with the Fugu genome data predicts that approximately 10,116 gene
109 also found when zfIFN was used to search the fugu genome database, demonstrating that zfIFN can be us
114 Our observations support the use of the Fugu genome to study vertebrate gene structure, to predi
119 for human, chimp, mouse, rat, zebrafish and fugu genomes are available for free download at http://w
120 etect HCTs in the human, mouse, chicken, and fugu genomes, and examined their association with cis-re
124 rthermore, we show for the first time that a Fugu genomic construct can produce protein in transgenic
126 contig maps have been constructed across two Fugu genomic regions containing the orthologs of human g
136 n, mouse, and pufferfish (Takifugu rubripes (Fugu)) have revealed a set of extremely conserved noncod
137 We have generated mice transgenic for the Fugu HD gene and conducted a detailed expression analysi
149 was used to engineer the spliceability of a Fugu intron in human cells by insertion of specific sequ
150 veloped to predict the splicing phenotype of Fugu introns in mammalian systems and was used to engine
154 e (bp) of all microsatellites, the genome of Fugu is similar to the genome of many other vertebrate s
156 ever, a teleost species such as zebrafish or Fugu is typically used as the outgroup in current tetrap
158 malian genome in a tail-to-tail orientation, Fugu IT and VT genes are linked head to tail and are sep
159 duced into the rat genome, we found that the Fugu IT gene was specifically expressed in rat hypothala
160 A contiguous stretch of 46 kb spanning the Fugu IT-VT locus has been sequenced, and nine putative g
163 emonstrated that the compact promoter of the Fugu lck contains regulatory elements that direct expres
167 t whole-genome shotgun assemblies reveal the Fugu MHC-related cluster of genes to be flanked predomin
171 efined threshold identifies 90% of all human/Fugu noncoding ECRs without the assistance of human-Fugu
172 ant number of evolutionarily conserved human-Fugu noncoding elements function as tissue-specific tran
173 erfish Takifugu rubripes (fugu), we isolated fugu orthologs of genes thought to be essential for limb
175 wed that each human receptor had one or more Fugu orthologs, excepting CAR (NR1I3) and LXRbeta (NR1H2
181 nd conservation of synteny between human and Fugu predict one gene to be an INK4A or INK4B homologue
183 absence of conserved promoter sequences, the Fugu promoter was found to be functional in mouse cells.
190 Interspecific comparison of the remaining fugu RH2-2 coding sequence paradoxically indicates that
191 k of frameshift or nonsense mutations in the fugu RH2-2 pseudogene suggests that the gene was lost ve
193 rafish with the same construct, we show that Fugu RNA is processed correctly in zebrafish but not in
194 s of the genome database for the pufferfish, Fugu rubrides, identified a goldfish ISG15 (gfISG15) hom
202 A sequence of the APP gene in the pufferfish Fugu rubripes and Tetraodon fluviatilis, respectively.
205 to the wnt1 gene of the Japanese pufferfish Fugu rubripes confirms the compact structure of the geno
212 esting preliminary data from analysis of the Fugu rubripes homolog of APP has shown an unusually high
213 a putative CB2 ortholog in the puffer fish (Fugu rubripes T012234) suggests it may encode a CBR othe
214 in more detail, we searched the pufferfish (Fugu rubripes) and zebrafish (Danio rerio) genomes for G
215 e compact genome of the Japanese pufferfish (Fugu rubripes) containing portions of three genes that h
217 then compared mouse, human, and pufferfish (Fugu rubripes) genomic sequences, and identified a conse
221 this locus between human, mouse, pufferfish (Fugu rubripes), and, in part, zebrafish (Danio rerio).
226 cterized the genomic structure of DRADA from Fugu rubripes, and compared the protein sequences of DRA
227 enes in the compact genome of the pufferfish Fugu rubripes, and examine the phylogenetic relationship
228 us of this unusual locus in the puffer fish, Fugu rubripes, and identified two INK4 genes using degen
229 ates, the mouse and the Japanese puffer fish Fugu rubripes, and investigated the genomic organization
231 FATPs are found in such diverse organisms as Fugu rubripes, Caenorhabditis elegans, Drosophila melano
232 lood coagulation scheme for the puffer fish, Fugu rubripes, has been reconstructed on the basis of or
234 h Caenorhabditis elegans and the puffer fish Fugu rubripes, suggesting that this eIF-2alpha kinase pl
235 ied the Hox gene clusters of a teleost fish, Fugu rubripes, to test the possibility that Hox organiza
247 are conserved in human-pufferfish, Takifugu (Fugu) rubripes, or ultraconserved in human-mouse-rat.
249 genome synteny views are available for each Fugu scaffold through the clonesearch web page located a
250 For each significant human gene match on the Fugu scaffold, the corresponding human chromosome map an
251 coding sequence is highly homologous to the Fugu sequence, suggesting that upregulation of CFTR in t
253 e both resources and data from the genome of Fugu so that everything may be integrated into a single,
255 mammalian and avian homologs except for the Fugu Surf-6 gene, which was found to lack an intron pres
257 These data demonstrate that the zebrafish:Fugu system is a powerful and convenient tool for dissec
258 ngle Hoxa2 gene in most vertebrates, whereas fugu (Takifugu rubripes) and medaka (Oryzias latipes) ha
259 er1b in zebrafish and per2a/per2b in madaka, fugu, tetraodon, and stickleback are ancient duplicates.
260 and E4TF1-60 are 49- and 24-fold smaller in Fugu than in human, and the intergenic distance is compr
262 ebrafish, medaka, threespine stickleback and fugu, the amphibian Xenopus tropicalis, the monotreme pl
263 iple sequence alignment of human, mouse, and Fugu, the putative WNT2 promoter sequence is shown to co
266 nerated from 15 different adult and juvenile Fugu tissues, 74% of which matched protein database entr
271 s suggests that the duplication event of the Fugu type 1A receptor may have occurred after the diverg
272 is loss in the pufferfish Takifugu rubripes (fugu), we isolated fugu orthologs of genes thought to be
273 a-regulated beta-type proteasome subunits in Fugu, which are present as a cluster within the Fugu MHC
274 g human, mouse, pig, Xenopus, zebrafish, and Fugu, with highly conserved nucleotide and deduced amino
275 otein in transgenic zebrafish: a full-length Fugu WT1 transgene with a C-terminal beta-galactosidase
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