ライフサイエンスコーパス: fumarase

1 ibition of fumarate hydratase (also known as fumarase).

2 nase, complex II, complex IV, aconitase, and fumarase.

3 by inactivating succinate dehydrogenase and fumarase.

4 ve also been seen in delta-crystallin and in fumarase.

5 sa possesses at least two and possibly three fumarases.

6 Fumarase A was overproduced.

7 hogluconate dehydratase, glutamate synthase, fumarase A, and FNR) and membrane-bound proteins (NADH d

8 um1Delta) lacking succinate dehydrogenase or fumarase activities.

9 ur mutants produced 1.7- to 2.6-fold-greater fumarase activity and 1.7- to 2.3-greater amounts of alg

10 espite displaying similar reductions in both fumarase activity and malate content as observed in toma

11 In vivo, fumarase activity fell to < 5% when cells were aerated;

12 A fumarase activity stain revealed that P. aeruginosa poss

13 Total fumarase activity was at least approximately 1.6-fold gr

14 ly resulting from absent or severely reduced fumarase activity, with currently unknown functional con

15 ferric chloride, demonstrated an increase in fumarase activity.

16 R was also used to randomly recombine CM and fumarase, an unrelated but also alpha-helical protein.

17 th mitochondrial succinate dehydrogenase and fumarase and activate the cytosolic ATP-citrate lyase in

18 are marked increases in transcripts encoding fumarase and fumarate reductase, enzymes putatively requ

19 n be synthesized from fumarate by the enzyme fumarase and further oxidized to oxaloacetate by malate

20 te oxidase, sarcosine oxidase and mixture of fumarase and sarcosine oxidase were used for monitoring

21 g argininosuccinate lyase, delta-crystallin, fumarase, and aspartase.

22 detected for ADP-glucose pyrophosphorylase, fumarase, and phosphoglucose isomerase activity.

23 atriuretic peptide receptor C, mitochondrial fumarase, and the 3',5'-cyclic nucleotide phosphodiester

24 atalytic efficiencies, such as chymotrypsin, fumarase, and urease, can be accurately and precisely es

25 The activities of fumarase- and manganese-cofactored superoxide dismutase

26 exposed to super-oxide at pH 6.5, cytosolic fumarase B was damaged.

27 e reductase (encoded by the frdABCD operon), fumarase B, which generates fumarate from malate, and th

28 n of the iron-sulfur proteins, aconitase and fumarase, by accumulated cytoplasmic NO.

29 elevated levels of one major transcript and fumarase C and manganase-cofactored SOD activity, sugges

30 Fumarase C catalyzes the stereospecific interconversion

31 The recent three-dimensional structure of fumarase C from Escherichia coli has identified a bindin

32 n-regulated, tricarboxylic acid cycle enzyme fumarase C is essential for optimal alginate production

33 The crystal structure of fumarase C with beta-(trimethylsilyl)maleate, a cis subs

34 otease,PrpL protease, exotoxin A, as well as fumarase C, Mn-dependent superoxide dismutase SodA, a fe

35 ater molecule may play an active role in the fumarase-catalyzed reaction.

36 The enzyme fumarase catalyzes the reversible hydration of fumarate

37 due His141, which is highly conserved in all fumarase class II enzymes and forms a charge relay with

38 numbering), acts as the general base in most fumarase class II superfamily members.

39 gions, which are moderately conserved in the fumarase class II superfamily, from three respective mon

40 PpCMLE is a homotetramer and belongs to the fumarase class II superfamily.

41 through M484, while the recombinant form, RY-fumarase, consists of residues S27 through L485.

42 additional crystallographic structures using fumarase crystals with bound inhibitors and poor substra

43 Fumaric aciduria (fumaric acidemia, fumarase deficiency) is a rare inborn error of metabolis

44 Furthermore, we found that the fumarase-dependent intracellular signaling of the B. sub

45 The crystal structure of the native form, NY-fumarase, encompasses residues R22 through M484, while t

46 parisons with other members of the aspartase-fumarase enzyme family, and the necessity for chemically

47 Whereas the resulting library contained fumarase fragments in many contexts before functional se

48 r both native and recombinant forms of yeast fumarase from Saccharomyces cerevisiae have been complet

49 provided by malate addition did not occur in fumarase (fum-1), glyoxylate shunt (gei-7), succinate de

50 acid fumaric acid, mediated by the cytosolic fumarase FUM2, is essential for cold acclimation of meta

51 e FH gene cause the deficiency of the enzyme fumarase (fumarate hydratase, EC 4.2.1.2) which result i

52 ine mutations of the fumarate hydratase (FH, fumarase) gene are found in the recessive FH deficiency

53 of this so-called B site of Escherichia coli fumarase had little effect on the overall initial rate k

54 (trimethylsilyl)maleate, a cis substrate for fumarase, has led to the discovery of the second site or

55 hanges in the active site of fumarase (yeast fumarase II) that occur when fumarate is converted to ma

56 o-2-hydroxypropionate, a strong inhibitor of fumarase in its carbanion form, is competitive with both

57 erference to down-regulate the expression of fumarase in potato (Solanum tuberosum) under the control

58 The reaction catalyzed by fumarase is critical for cellular energetics as a part o

59 Fumarase is distributed between two compartments of the

60 the dual function of the ancient prokaryotic fumarase, led to its subsequent distribution into differ

61 nthase, aconitase, isocitrate dehydrogenase, fumarase, malate dehydrogenase, and succinate dehydrogen

62 levated again in succinate dehydrogenase and fumarase mutants, and diminished again in malate dehydro

63 Here, we show that fumarase of the model prokaryote Bacillus subtilis (Fum-

64 In vitro analysis demonstrated that the fumarase of the succinate-propionate pathway contains an

65 are measured using alcohol dehydrogenase or fumarase plus malic dehydrogenase reactions, respectivel

66 A catalytic mechanism for the fumarase reaction that can account for the kinetic behav

67 active site has been a major mystery of the fumarase reaction.

68 activity invariably contained a CM core with fumarase sequences found only at the termini or in one l

69 odeling based on the known structures in the fumarase superfamily, including argininosuccinate lyase,

70 highly conserved regions within the class II fumarase superfamily.

71 the inactivation of the [4Fe-4S]-containing fumarases that otherwise occurs in the sodA sodB strain.

72 Recycling of yeast fumarase to permit repetition of its reaction chemistry

73 We report the discovery of fumC, encoding a fumarase, upstream of the sodA gene, encoding manganese

74 As a comparison, native fumarase was crystallized in the presence of the competi

75 as been demonstrated by studying crystalline fumarase with the bound competitive inhibitors-citrate a

76 Changes in the active site of fumarase (yeast fumarase II) that occur when fumarate is