ライフサイエンスコーパス: functional expression

1 of four sodium channel subunits) with robust functional expression.

2 membrane region with hERG and decreases hERG functional expression.

3 ed, resulting in lower levels of protein and functional expression.

4 g transport to the cell surface and thus its functional expression.

5 R both in vitro and in vivo to ensure proper functional expression.

6 l aggregates (hMSC spheroids) restores CXCR4 functional expression.

7 Kv1.2 cell-surface expression efficiency and functional expression.

8 s were activated by light pulses, indicating functional expression.

9 post-translational modifications to achieve functional expression.

10 s, whereas murine or rat ENaC increases CFTR functional expression.

11 hat necessitates mammalian systems to obtain functional expression.

12 whereas the presence of ENaC increases CFTR functional expression.

13 the fenamate niflumic acid, confirming their functional expression.

14 fy this enzyme by transcriptome analysis and functional expression.

15 own transcripts was validated by cloning and functional expression.

16 itions in the natural sequence that restrict functional expression.

17 ased total Hsp70 expression ~2-fold and ENaC functional expression ~1.4-fold.

18 activates CAR at the BBB and increases P-gp functional expression, a clinically significant drug-dru

19 in both plant hosts, and metatranscriptomic (functional expression) analysis revealed that most genes

20 neuronal CaV1.2 channels in vivo: increased functional expression, anchoring of AKAP15 and PKA, and

21 bunits has a gain-of-function effect (higher functional expression and agonist sensitivity and sponta

22 Functional expression and analysis of AaCAT1 in Xenopus

23 ta2alpha4)2 nAChRs yielded similar levels of functional expression and Ca(2+) permeability, measured

24 Functional expression and complementation of temperature

25 ntactin may thus significantly influence the functional expression and distribution of Na(+) channels

26 Herein we describe the cloning, functional expression and initial characterization of OR

27 Our results suggest that functional expression and pharmacological studies may pr

28 As age progresses, alpha subunit functional expression and protein levels diminish in cor

29 The functional expression and redistribution of endothelial

30 tatic regulation of CaV2.1 protein level and functional expression and that RNF138 serves as the prim

31 The high level of functional expression and the low level of endogenous co

32 microelectrode voltage-clamp, we report the functional expression and the molecular, biophysical, an

33 h the increasing availability of large scale functional, expression and evolutionary data.

34 body of previous work using mutagenesis and functional expression, and is now being supplemented by

35 hannel gating, and several T1 sites regulate functional expression as well.

36 te umami taste receptor hTAS1R1-hTAS1R3 in a functional expression assay.

37 n vitro, and respond to bitter tastants in a functional expression assay.

38 manipulate plant processes, high-throughput functional expression assays in plants were performed on

39 tags (ESTs) with virus-based high-throughput functional expression assays in plants.

40 candidate genes enhanced the DeltaF508-CFTR functional expression at the apical PM in human CF bronc

41 ) has been identified as a regulator of P-gp functional expression at the BBB.

42 fied in our screen likely impact Kir-channel functional expression at the level of vesicle budding fr

43 udy is to investigate the regulation of BCRP functional expression by peroxisome proliferator-activat

44 conjunction with a reporter gene creating a functional expression cassette.

45 les and also class I MHC molecules for their functional expression, characterization and engineering.

46 Through a functional expression cloning approach, a gag-retinoid X

47 Functional expression cloning is a powerful strategy for

48 Using functional expression cloning, we have identified GAS5 (

49 bait, we show the feasibility of a modified functional expression-cloning strategy to identify human

50 We studied their trafficking and functional expression, combining immunocytochemical and

51 Changes in ENaC functional expression correlated with ENaC surface expre

52 This increase in ENaC functional expression corresponded to an increase in ENa

53 so on molecular genetic analysis and ideally functional expression data.

54 ine 508 (DeltaF508 or Phe508del), results in functional expression defect of the CF transmembrane con

55 Cystic fibrosis (CF) is caused by the functional expression defect of the CF transmembrane con

56 by targeted disruption of a PKS gene, and by functional expression in a heterologous Streptomyces hos

57 reased processing, and impaired cell surface functional expression in Calu-3 human airway epithelial

58 ons into the orthologous mouse ZIP4 gene for functional expression in cultured cells.

59 tically enhances DeltaF508 CFTR cell surface functional expression in cystic fibrosis airway epitheli

60 length dehydrogenase clones were screened by functional expression in Escherichia coli using a recent

61 rescent protein (YFP) and first assessed for functional expression in HEK293 cells.

62 en homomeric subunits as expected from their functional expression in heterologous expression systems

63 Functional expression in heterologous system and gene kn

64 ther ion channels have been characterized by functional expression in heterologous systems, and most

65 Functional expression in human embryonic kidney 293 cell

66 sideration when assessing alterations of its functional expression in pathobiological states.

67 Screening of these candidate genes by functional expression in Saccharomyces cerevisiae yielde

68 ingosine phosphate lyase gene along with its functional expression in Saccharomyces cerevisiae.

69 1 voltage-gated K(+) channels) have enhanced functional expression in smooth muscle cells of a primar

70 activity-dependent (auto)regulation of VGSC functional expression in the strongly metastatic Mat-LyL

71 functions because of an inability to obtain functional expression in vitro.

72 tal C-terminal domain is required for normal functional expression in vivo.

73 Functional expression in Xenopus laevis oocytes followed

74 Functional expression in Xenopus oocytes of concatenated

75 NKCC2B variant into the A or F variant, with functional expression in Xenopus oocytes.

76 Functional expression in yeast and Xenopus oocytes revea

77 assigned as a taxane 10 beta-hydroxylase by functional expression in yeast.

78 is genome by direct enzymological assays and functional expression in yeast.

79 to plasma membrane for surface insertion and functional expression is a key process regulating signal

80 We demonstrate here that the lack of functional expression is due to failure of the mutant ch

81 ssociated with decreased Kir4.1 K(+) channel functional expression, leading to elevated in vivo stria

82 that such interaction depends critically on functional expression level (controlled by RNA injection

83 channel activation, kinetic properties, and functional expression levels to be investigated with sin

84 Our study has defined the "MECP2 functional expression module" and identified enhancer an

85 olin-1 coexpression reduced Slo1 surface and functional expression near 70% without affecting channel

86 We examined the in vivo functional expression of 12 selected receptors by microi

87 The functional expression of 6msas can be used to activate d

88 The functional expression of 95 genes was evaluated in mice

89 Previous studies have suggested an increased functional expression of a cell-surface import system fo

90 e best of our knowledge the first example of functional expression of a cyanobacterial P450 in E. col

91 We thus report the cloning and functional expression of a gene involved in an insect-sp

92 ystem to identify proteins necessary for the functional expression of a mammalian Kir channel at the

93 This work represents the full sequencing and functional expression of a marine natural-product pathwa

94 Here we describe the molecular cloning and functional expression of a novel member of the serpin fa

95 Functional expression of a selection Nostoc spp.

96 igonucleotides inhibited NMD and rescued the functional expression of a third LQT2 mutation, Y1078.

97 these structural alterations did not impair functional expression of AE1, the cytosolic and membrane

98 virus have been evaluated for high-level and functional expression of AhR.

99 Functional expression of alpha-amino-3-hydroxy-5-methyl-

100 To test the hypothesis that increased functional expression of alpha4* nAChRs in these neurons

101 SIC2 expression, accounting for the observed functional expression of amiloride-sensitive currents in

102 insertion of adrenergic receptors as well as functional expression of AMPA and NMDA receptors at syna

103 ist DNQX, indicating that 5-HT increased the functional expression of AMPA-type receptors in the moto

104 novel, unidentified estrogen receptor or to functional expression of an estrogen receptor-alpha spli

105 d intracellularly and that it is the lack of functional expression of ASIC2 at the cell surface that

106 the hypothesis that the AE3 knockout reduces functional expression of astrocytic NBCe1.

107 These observations indicate a functional expression of AtGLR3.5 in this organelle.

108 Differences were found in the functional expression of ATP receptors, TRPA1 channels,

109 n that of TASK-1 and TASK-3, consistent with functional expression of both channel types.

110 RgIA inhibition of Ca(v)2.2 channels needed functional expression of both human GABA(B) receptor sub

111 oocytes, ERp29 overexpression increased the functional expression of both wild-type and DeltaF508-CF

112 omain disrupted cell surface trafficking and functional expression of Ca(V)2.3.

113 h that resulted in the molecular cloning and functional expression of CCH1 by exploiting homologous r

114 Thus, the functional expression of CCR6 is sufficient to provide t

115 Here, we report the identification and functional expression of CD38 from Xenopus laevis, a key

116 together, these observations demonstrate the functional expression of CD40 in epithelial tumors of th

117 ce that disease-associated or injury-induced functional expression of chemokines/receptors in both ne

118 rones promote both the protein level and the functional expression of CLC-1 wild-type and A531V mutan

119 Functional expression of Clock, a circadian clock gene t

120 e sulphonylurea receptor SUR2A can alter the functional expression of cloned ATP-sensitive K(+) chann

121 r biocytin were integrated in a study of the functional expression of corticotropin-releasing factor

122 Functional expression of COX-2 was confirmed by Northern

123 Functional expression of Coxiella bcp was demonstrated b

124 contribute to tumor growth by modulating the functional expression of critical genes involved in tumo

125 Ad-SM22-4A1 drives a smooth muscle-specific functional expression of CYP4A1 and demonstrates increas

126 We show here that dexamethasone rescues functional expression of DeltaF508-CFTR.

127 lection fluorescence microscopy to study the functional expression of DOR in sensory neurons from rat

128 in is shown to exhibit 50% dependence on the functional expression of dynamin 2 for its active cellul

129 signaling by the EP(4) receptors induces the functional expression of early growth response factor-1

130 In support of this hypothesis, functional expression of Erg channels in a heterologous

131 neuronal membrane excitability by regulating functional expression of Erg channels.

132 have opened the way for the high-throughput functional expression of eukaryotic membrane proteins.

133 Direct proof for functional expression of FcgammaR by Ag-specific CD8 T c

134 A recent study demonstrated functional expression of FFA3 in the rodent sympathetic

135 tion signal and was essential for detectable functional expression of FLP15-R in CHO cells.

136 The functional expression of FLP15-R in mammalian cells was

137 systems is particularly detrimental for the functional expression of G protein-coupled receptors (GP

138 nels that deactivate upon hyperpolarization, functional expression of G499R ClC-1 yielded a hyperpola

139 Treatment with genistein both enhanced the functional expression of G551D-CFTR and improved regulat

140 e in the pathology of SE are deficits in the functional expression of GABA(A) receptors (GABA(A)Rs),

141 Chronic ethanol exposure increased the functional expression of GABA(A) receptors in acutely is

142 and that Orai1 and STIM1 expression leads to functional expression of Gd-resistant ROCE.

143 e D1 receptor signaling are required for the functional expression of GluN2B transmission and to sust

144 We show that functional expression of GLUT5 in yeast requires mutatio

145 We found that functional expression of glutamate transporter GLT1 is 4

146 phenotypic expression of CD11a and increased functional expression of granzyme B and interferon-gamma

147 The results indicate that functional expression of HCN3 channels in IGL neurons is

148 reticulum (ER), thus accounting for the poor functional expression of homomeric 1b currents.

149 Mutating the RXR facilitated maturation and functional expression of homomeric hERG 1b channels in a

150 duces cell-surface expression efficiency and functional expression of homomeric Kv1.2 channels.

151 Despite recent progress in the functional expression of hT2Rs in vitro, up until now, h

152 microtubule-based trafficking processes for functional expression of hTHTR1.

153 Such feedback mechanism between the functional expression of I(A) and intracellular Ca(2+) m

154 vidence to demonstrate that the kinetics and functional expression of I(A) are tightly regulated by i

155 athematical modeling, we determined that the functional expression of IKD is reduced in sensory neuro

156 ration, indicating that VEGF/VPF induced the functional expression of IL-8 protein in HBMECs.

157 expression, we have investigated whether the functional expression of iNOS regulates oral tolerance.

158 We have demonstrated that the morphology and functional expression of ionic currents in trans-differe

159 Here we show that the functional expression of K(Ca) channels in LMNs developi

160 erum into the embryonic hindlimb reduced the functional expression of K(Ca) channels in vivo to level

161 1 at the plasma membrane and is required for functional expression of K(V)10.1 channels.

162 ity act via a common pathway that influences functional expression of K+ channels and proliferative c

163 r mechanism that neurons use to modulate the functional expression of KCC2 remains rudimentary.

164 R (mean~800 Omega cm(2)); it also shows good functional expression of key tight junction proteins, tr

165 itronectin and were consistent with enhanced functional expression of Kv4-family subunits.

166 mechanisms at the cell surface regulate the functional expression of Kv4.2 channels.

167 The functional expression of large-conductance (BK-type) Ca2

168 The functional expression of large-conductance Ca2+-activate

169 data provide direct evidence supporting the functional expression of M cells in intact myocardium an

170 he genomic structure of murine ADAT1 and the functional expression of mADAT1 cDNA.

171 rrowed the search for domains that influence functional expression of malpha6*-nAChRs.

172 ts are some of the molecular impediments for functional expression of malpha6mbeta2beta3- or malpha6m

173 s is the first demonstration of heterologous functional expression of mammalian OPN4 in the genetical

174 Functional expression of markers requires Cre-mediated i

175 and respond to bacteria by demonstrating the functional expression of members of the Toll-like family

176 rone machinery regulates the homeostasis and functional expression of misfolded proteins in the cell.

177 direct association with p11 is required for functional expression of Na(V)1.8, disrupting this inter

178 sis of caged carbachol demonstrated that the functional expression of nAChRs is located both on the s

179 e that association with CaM is important for functional expression of NaV1.4 and NaV1.6 VGSCs.

180 clude that there is spatial variation in the functional expression of NBTI/dipyridamole-sensitive tra

181 Here we show that the functional expression of neuronal N-type Ca(V) channels

182 As we learn more about the functional expression of nicotinic receptors in specific

183 transfections, was essential for detectable functional expression of NPR-1.

184 N-terminal amino acids was not required for functional expression of OEP80 and accumulation of the 7

185 s result may be at least in part due to poor functional expression of olfactory receptors and/or limi

186 This gene (ODR-4) allows for functional expression of olfactory receptors in heterolo

187 Thus, an increase in functional expression of one channel reciprocally modula

188 tion bias of long-range connections with the functional expression of orientation selective neural ac

189 though hypersensitivity to NSC73306 required functional expression of P-gp, biochemical assays reveal

190 ere was no electrophysiological evidence for functional expression of P2X4 receptors on AgRP-NPY neur

191 ken together, the present study demonstrated functional expression of P2X7 receptors in non-neuronal

192 region of Pax3 that are sufficient to direct functional expression of Pax3 in neural crest.

193 dy, we report that Galpha12QL stimulates the functional expression of PDGFRalpha and demonstrate that

194 Functional expression of PfNT1 in Xenopus laevis oocytes

195 We examined cellular protein processing and functional expression of photoreceptor cyclic nucleotide

196 By examining the functional expression of plasma membrane transporters at

197 are generally obligatory events required for functional expression of prenylated proteins.

198 Both phenytoin and lamotrigine increased functional expression of R1648C, but lamotrigine also in

199 e wild-type 5-HT(3)A subunit, did not affect functional expression of receptors, suggesting that the

200 d antibody staining were employed to confirm functional expression of recombinant rho1 receptors afte

201 to pathological hypertrophy, therefore, the functional expression of repolarizing K(+) (and depolari

202 TP1, RTP1S, supports robust cell-surface and functional expression of representative odorant receptor

203 We obtained functional expression of Sav1866 in the drug-sensitive,

204 tomy suggests that GRP94 is required for the functional expression of secretory and/or membrane prote

205 suggesting an essential role for Gp93 in the functional expression of secretory/integral membrane pro

206 SERT substrates also shifted the relative functional expression of SERT by redistributing intracel

207 Despite the cloning and functional expression of several Ca(2+) channels, those

208 Here, we report functional expression of SHANK3 in mouse dorsal root gan

209 mice demonstrates that ROMK is essential for functional expression of SK channels in both TAL and CCD

210 sodA sodB-deficient mutant, demonstrated the functional expression of SOD activity by cloned M. catar

211 In this study, we demonstrate that functional expression of Stil is also required for neura

212 ow cell density is correlated with increased functional expression of TGF-beta receptors and promotio

213 3 ligase activity significantly enhanced the functional expression of the A531V mutant.

214 past studies indicating that NPY affects the functional expression of the alpha1B-adrenergic cascade

215 Using S. cerevisiae as host for the functional expression of the C. albicans Fe-uptake prote

216 he biliary output of bile salts (BS) and the functional expression of the canalicular BS export pump

217 strongly inhibited CDK2 activity, indicating functional expression of the CDK inhibitors in both cell

218 Functional expression of the cDNAs in Escherichia coli s

219 K(ATP) channels via SUR1, thereby affecting functional expression of the channel in beta-cell membra

220 ences in the voltage-dependent phenotype and functional expression of the channel.

221 e calcium channels, which leads to increased functional expression of the channel.

222 ouabain-resistant colonies, consistent with functional expression of the chimeric protein and indica

223 Here, we report that functional expression of the Eag channel is temperature-

224 The functional expression of the G protein-coupled P2Y(2) nu

225 esponse to neuronal injury by regulating the functional expression of the glutamate receptor subunits

226 lls was unaffected by these peptides despite functional expression of the high-affinity fMLP receptor

227 tudy, we directly compared mRNA, protein and functional expression of the high-conductance Ca(2+)-act

228 tional assay also confirmed the cleavage and functional expression of the HIV-1 reverse transcriptase

229 The experiments were comprised of functional expression of the hKAT-I in an insect cell/ba

230 We here report the cloning and the functional expression of the human intestinal LfR and th

231 We used whole cell recording to evaluate functional expression of the intermediate conductance Ca

232 , identified seven deletion strains in which functional expression of the Kir channel at the plasma m

233 adrenergic receptor (beta(2)-AR) facilitates functional expression of the OR M71 at the plasma membra

234 hloride homeostasis due to a decrease in the functional expression of the potassium-chloride cotransp

235 r a late synthetic promoter allowed a higher functional expression of the protein and much stronger (

236 Functional expression of the R672S channels in human emb

237 We report herein the first cloning and functional expression of the rat 175-kDa HARE.

238 ve been severely hampered by the inefficient functional expression of the receptors in heterologous e

239 was ENaC-specific, as PON-2 had no effect on functional expression of the renal outer medullary potas

240 induced Ca(2+) release and thereby confirmed functional expression of the RYR in the cell lines expre

241 es in cortical brain slices to show that the functional expression of the slow calcium-activated afte

242 ith activation of Akt and STAT5 and required functional expression of the small GTPases Rho, Rac, and

243 Functional expression of the StV-specific MERG1a channel

244 Functional expression of the telomerase catalytic subuni

245 Significantly, we found that reduced functional expression of the voltage-dependent potassium

246 t ion channels are defined experimentally by functional expression of their pore-forming alpha subuni

247 poor, in part because of limited success in functional expression of these channels.

248 Functional expression of these enzymes in yeast was moni

249 rappreciated, in part, due to relatively low functional expression of these receptors in naive states

250 se construct of the Cl3'NT/NU to dissect the functional expression of these two surface membrane enzy

251 Functional expression of this alpha1G isoform in Xenopus

252 y play a central role in modulating both the functional expression of this critical transporter in th

253 Functional expression of this receptor in human breast c

254 sensitive NMDA receptors, and found that the functional expression of this receptor subtype is strain

255 Interestingly, the functional expression of this transporter at the presyna

256 amined the effect of membrane cholesterol on functional expression of tissue factor (TF), a cellular

257 induced CXCL10 production, demonstrating the functional expression of TLR4 by osteoblasts.

258 ite-directed mutagenesis in combination with functional expression of transporter mutants in Xenopus

259 Functional expression of TRP channels was determined wit

260 ted by distinct blockers consistent with the functional expression of TRPM7 channels.

261 Our data demonstrate functional expression of TRPM7-like channels in mouse oo

262 The functional expression of TRPV1 on airway epithelium has

263 By the functional expression of tsNa(V)1.4, we show how differe

264 tosynthetic rate increases in leaves and the functional expression of tvinv is crucial for such effec

265 ector VRT-325, could rescue cell surface and functional expression of two representative Na(V)1.1 mut

266 er transition from C to U and, therefore, on functional expression of uracil N-glycosylase 2, which i

267 cell capture assay to assess changes in the functional expression of vascular endothelial growth fac

268 To examine the relationship of functional expression of VLA-4 to prognosis in AML, we s

269 This study asked whether the functional expression of voltage-gated Ca(2+) channels (

270 eshold of cold thermosensors correlated with functional expression of voltage-gated K channels Kv1.1/

271 Functional expression of voltage-gated Na(+) channels (V

272 Functional expression of VR.5'sv in Xenopus oocytes and

273 physically associate with (and regulate the functional expression of) mouse CD1d on the surface of c

274 Functional expressions of ObAS1 and ObAS2 in Saccharomyc

275 that the ephrin-B2 ligand is likely to have functional expression on angiogenic arterial ECs and ind

276 ions, atherosclerotic plaque deposition, and functional expression on worsening outcomes of women.

277 les introduce the possibility that selective functional expression patterns may provide correlates fo

278 To elucidate the functional expression patterns of these loci, adoptive t

279 ionship between gene-island distribution and functional expression profiling suggests for the first t

280 t the employment of Escherichia coli for the functional expression, purification, and reconstitution

281 In stomata, loss of OsK5.2 functional expression resulted in lack of time-dependent

282 ine phosphorylation sites by mutagenesis and functional expression revealed a multisite regulatory me

283 Functional expression reveals that G406R produces mainta

284 ed insulin resistance and type 2 diabetes, a functional expression screen was performed to search for

285 These pseudogenes can be recombined into the functional expression site to generate new antigenic var

286 plasmic reticulum of cells commonly used for functional expression studies and are only released to t

287 H) for CaSR mutations and performed in vitro functional expression studies and three-dimensional mode

288 Functional expression studies in Xenopus oocytes demonst

289 A functional expression survey of nearly 200 candidate gen

290 receptor sorting to the plasma membrane for functional expression under normal and pathological cond

291 olves a parallel decrease of alpha and beta1 functional expression via a transcript downregulatory me

292 Intracellular labeling was decreased while functional expression was confirmed by whole cell patch

293 The lack of SV1 functional expression was due to endoplasmic reticulum (

294 The increase in functional expression was not due to a change in the rat

295 Functional expression was successful, with uptake of Ca(

296 The differences in functional expression were associated with changes in bo

297 CRACM mRNA, protein, and functional expression were examined in purified HLMCs an

298 -negative effect of EA2 mutants on CaV2.1 WT functional expression, which can be attributed to defect

299 The increase in mENaC functional expression with coinjection of 10 ng of Hsp70

300 ly developed FACS-based selection system for functional expression with ultradeep 454 sequencing, we