ライフサイエンスコーパス: fusome

1 art of the germ-cell-specific organelle, the fusome.

2 n filaments to accumulate ectopically in the fusome.

3 rin transits are tightly associated with the fusome.

4 Short stop (Shot) as a new component of the fusome.

5 monopolar, and do not make contact with the fusome.

6 cally required for PAR-1 localization to the fusome.

7 ems to depend instead on the polarity of the fusome.

8 r Dynein heavy chain 64C, which disrupts the fusome.

9 l for the localization of Bam protein to the fusome.

10 ntrolling localization of Bam protein to the fusome.

11 al and gradually fuses with the pre-existing fusome.

12 disrupted in hts(1) mutant cysts, which lack fusomes.

13 These proteins are also components of the fusome, a cytoplasmic structure that spans the cystoblas

14 o orient GSC division through capture of the fusome, a germline-specific organizer of mitotic spindle

15 Our results suggest that the fusome, a specialized membrane-skeletal structure, which

16 ividing cysts by following the growth of the fusome, a vesiculated cytoplasmic organelle.

17 microtubules are seen to associate with the fusome, an asymmetric germline-specific organelle, which

18 Mutants of the fusome, an organelle that is known to facilitate intracy

19 RNA, specifically associate with the oocyte fusome and a region of the Balbiani body prior to becomi

20 assembly of membranous cisternae within the fusome and block cystoblast differentiation altogether.

21 Shot associates with the fusome and is required for microtubule organization.

22 Our results suggest that the fusome and its associated membrane skeleton play a centr

23 Balbiani body development requires an intact fusome and microtubule cytoskeleton as it is blocked by

24 Shot provides the missing link between the fusome and microtubules within meiotic cysts, which is e

25 il to maintain the branched structure of the fusome and periodically show improperly localized oocyte

26 The PAR-1 protein is localized to the fusome and ring canals within the developing germline cy

27 female-sterile mutant that affects both the fusome and ring canals.

28 provides a link between the asymmetry of the fusome and the selection of the oocyte.

29 for the association of microtubules with the fusome and the subsequent specification of the oocyte in

30 In htsl cysts which lack fusomes and fail to incorporate a hts gene product(s) in

31 se observations support the proposed role of fusomes and ring canals in organizing a polarized microt

32 e unfertilized products of meiosis, with the fusomes and spectrosomes that anchor the spindles of div

33 tach from niche cap cells, contain displaced fusomes and undergo abnormal cell division, leading to a

34 ct defects: disrupted intercellular bridges (fusomes) and premature centriole disengagement.

35 Finally, we show that PAR-1 localises on the fusome, and provides a link between the asymmetry of the

36 Spectrosomes and fusomes are associated with one pole of spindles during

37 mutants, the stem cells and their associated fusomes are eventually lost as Orbit/Mast protein is dep

38 Moreover, they reinforce the notion that fusomes are required for the four synchronous divisions

39 These observations suggest that fusomes are required for the proper formation and asymme

40 also associate with the fusome, first on the fusome arms and then in its center, subsequently moving

41 ost-mitotic cells differentiate and lose the fusome as F-actin-rich ring canals form.

42 rogressively focus towards the center of the fusome, as visualized using a NOD-lacZ marker.

43 g the cystocyte cell cycles, suggesting that fusome-associated Cyclin A drives the interconnected cel

44 We also show that fusome association of TER94 is Bam-dependent, suggesting

45 ion of Cyclin A/Cdk1 is essential for normal fusome behavior and centriole engagement during premeiot

46 f patterning determinants, oocyte growth and fusome biogenesis in males and females.

47 We propose that fusome biogenesis is an obligate step for cystoblast cel

48 t similar to that of other components of the fusome but instead is similar to the absence of the down

49 KLP61F proved to be maintained in fusomes by microtubule-independent, detergent-resistant

50 Our studies reveal that fusomes change during development and contain recycling

51 To understand the relationship between fusome cisternae and cystoblast differentiation, we have

52 The presence of TER94 suggests that the fusome cisternae grow by vesicle fusion and are a germ c

53 A significant number of fusome components are dispensable, because genetic disru

54 lthough mutations in genes encoding skeletal fusome components prevent proper cyst formation, mutatio

55 We found that fusomes contain two additional membrane skeletal protein

56 ed by a large cytoplasmic structure called a fusome, containing alpha-spectrin and the adducin-like p

57 version of Ovhts delays the transition from fusome-containing cells to those that have ring canals.

58 y within dividing ovarian cysts requires the fusome cytoskeletal component and suggest a possible rol

59 Inspection of fusome-deficient hu-li tai shao (hts) mutants indicated

60 ao (hts) mutants indicated that KLP61F gains fusome-dependent interactions near telophase that mediat

61 tor Cyclin A transiently associates with the fusome during the cystocyte cell cycles, suggesting that

62 F cycles between spindles during mitosis and fusomes during interphase.

63 of protein trap lines, we identified 14 new fusome-enriched proteins, including many associated with

64 and a branched cortical structure known as a fusome extrudes through intercellular bridges called rin

65 ystocyte centrosomes also associate with the fusome, first on the fusome arms and then in its center,

66 llular and molecular processes that underlie fusome formation and cyst initiation, construction, and

67 n accumulation of cortical actin, defects in fusome formation, mislocalization of septins, defective

68 Our studies show that the fusome grows by a regular, polarized process throughout

69 ound of mitosis, a membranous organelle, the fusome, grows along the cleavage furrow and the remnants

70 oogenesis a membranous organelle called the fusome has a key function in the establishment of oocyte

71 e asymmetry of GSC division; eliminating the fusome in developing cysts results in defective spindles

72 Whereas Ovhts-Fus localizes to the fusome in mitotic cells, Ovhts-RC localizes to ring cana

73 ine for synchronized germline cell division, fusome integrity and oocyte differentiation.

74 u-li tai shao (Hts) are required to maintain fusome integrity, synchronize asymmetric cystocyte mitos

75 This ensures correct growth of the fusome into a branched asymmetrically distributed organe

76 The Drosophila fusome is a germ cell-specific organelle assembled from

77 The fusome is a germline-specific organelle that is associat

78 nd the proteasome 19S-RP subunit S1 with the fusome is affected in encore mutant germaria.

79 correctly in such cells and the structure of fusome is compromised.

80 of CLIP-190 to such microtubules and to the fusome is dependent upon Orbit/Mast to which it is compl

81 The fusome is essential for the pattern and synchrony of the

82 The fusome is necessary for the microtubule-driven restricti

83 canal is transient and closes down after the fusome is partitioned through it.

84 The fusome is required for this complex process, because mic

85 and oocyte differentiation; the role of the fusome itself, however, and the organization and functio

86 multaneously fortifying GSC-niche junctions, fusome localization and asymmetric cell division.

87 indicated that KLP61F is required to recruit fusome material to spindle midbodies near telophase and

88 fate and that Bam is the limiting factor for fusome maturation in female germ cells.

89 reduced association of Cul1 and S1 with the fusome may compromise Cyclin E destruction and consequen

90 tent of the spectrosome, suggesting that the fusome mediates intercellular signals that depend on the

91 This fusome-microtubule association occurs periodically durin

92 This correlates with abnormal fusome morphology and arrested germ cell development in

93 ndria and Golgi vesicles associates with the fusome, moves through the ring canals, and enters the oo

94 bly and microtubule-independent functions in fusome organization.

95 ndle midbodies near telophase and for normal fusome organization.

96 o the oocyte, but the mechanism by which the fusome organizes the microtubules is not known.

97 In the presence of a normal fusome, overexpression of Cyclin A forces cysts through

98 The fusome plays an essential role in prefollicular germ cel

99 In dividing cysts, as the fusome plugs move toward the pre-existing fusome, their

100 s that have examined inactivating alleles of fusome proteins indicate that the organelle plays centra

101 tubule cytoskeleton, which together with the fusome provides polarity within the developing germline

102 At the end of each cycle of cyst growth, the fusome remains asymmetrically distributed within the cys

103 cystoblast differentiation may be linked to fusome reticulum biogenesis.

104 larization cycle begins in mitosis, when the fusome segregates to a single daughter cell of each pair

105 n-1 heavy chain, or scribble, does not alter fusome structure or female fertility.

106 al spindle (spindle remnant), ring canal and fusome, suggesting it participates in interactions betwe

107 h four ring canals retains a bigger piece of fusome than any other cell, including the other cell wit

108 s is more dependent on alpha-spectrin in the fusome than at the plasma membrane in other cells.

109 ontain a structure similar to the Drosophila fusome that that is probably involved in anchoring of th

110 expanded number of GSC-like cells with round fusomes that display ectopic BMP signal responsiveness a

111 period contain a distinctive organelle, the fusome, that is required for normal cyst formation.

112 he fusome plugs move toward the pre-existing fusome, their associated ring canals also move, changing

113 ain GSC-cap cell junctions and to anchor the fusome to the anterior cortex of the GSC.

114 o identify other proteins in this network of fusome tubules.

115 intercellular connectivity supported by the fusome uniquely increases the sensitivity of the germlin

116 and contain a specialized organelle called a fusome, whereas later post-mitotic cells differentiate a

117 s to facilitate multiple interactions of the fusome with mitotic spindles and ring canals.