ライフサイエンスコーパス: galactokinase

1 phosphate kinase (GalAK) and compare it with galactokinase.

2 served among both prokaryotic and eukaryotic galactokinases.

3 ces available in the literature thus far for galactokinases.

4 ptive human galactokinase cDNA, had very low galactokinase activity even when yeast were grown on gal

5 genes expressed tetracycline resistance and galactokinase activity in vitro and in vivo at significa

6 80p and Gal3p mutants as well as the lack of galactokinase activity of Gal3p.

7 xpected from amino acid sequence alignments, galactokinase adopts a similar topology to that observed

8 The assay couples the galactokinase and firefly luciferase reactions.

9 alactosamine is prepared enzymatically using galactokinase and galactose-1-phosphate uridyltransferas

10 a, activities and substrate specificities of galactokinase and GalNAc kinase from human and pig kidne

11 s, the three-dimensional structures of human galactokinase and two bacterial forms of the enzyme have

12 ally engineered into a heterologous protein (galactokinase) and the relative processing of these subs

13 This enzyme is clearly separated from galactokinase by chromatography on phenyl-Sepharose.

14 a suitable PET tracer for measuring hepatic galactokinase capacity in vivo, which provides estimates

15 nts showed that high level expression of the galactokinase cDNA did not complement the peroxisomal as

16 st clone, transfected with presumptive human galactokinase cDNA, had very low galactokinase activity

17 The galactokinase clone was shown to be functional, as it co

18 that the molecular basis for this particular galactokinase deficiency is an increase in the K(m) for

19 iency, which can lead to early fatality, and galactokinase deficiency, which causes cataracts.

20 The structure of galactokinase described here serves as a model for under

21 describe the three-dimensional structure of galactokinase from Lactococcus lactis determined to 2.1-

22 In addition, removal of the Gal1p galactokinase from the network resulted in a regimed res

23 ase from human and pig kidney, as well as of galactokinase from the yeast clone transfected with the

24 Galactokinase functions in the Leloir pathway for galact

25 mber of the GHMP kinase family that includes galactokinase (G), homoserine kinase (H), mevalonate kin

26 oned cDNA was placed under control of the Sc galactokinase (GAL1) promoter and restored P5CR activity

27 visiae that encode a co-inducer (GAL3) and a galactokinase (GAL1).

28 With galactokinase Gal1p and transcriptional activator Gal4p

29 Despite being considerably larger than other galactokinases, Gal1p shares a similar molecular archite

30 eloir pathway and requires the three enzymes galactokinase, galactose-1-P uridylyltransferase, and UD

31 the Leloir pathway for galactose metabolism (galactokinase, galactose-1-phosphate-uridyl transferase

32 talytic capabilities of the Escherichia coli galactokinase GalK.

33 Galactokinase (GalK) catalyses the first step of the Lel

34 Galactosemia II results from loss of galactokinase (GALK), phosphorylating galactose to galac

35 ncode tetracycline resistance [tetA(Q)2] and galactokinase (galK).

36 Here we show that the Escherichia coli galactokinase gene (galK) can be used as a counterselect

37 tant (KmR) gene for positive screening and a galactokinase gene (galK) for negative screening.

38 In humans, mutations in the galactokinase gene can lead to the diseased state referr

39 Galactokinase (GK; EC 2.7.1.6) is the first enzyme in th

40 e clinical consequences, and deficiencies in galactokinase have been linked with the development of c

41 nces and three-dimensional structures of the galactokinase, homoserine kinase, mevalonate kinase, and

42 f the GHMP family of kinases, which includes galactokinase, homoserine kinase, mevalonate kinase, and

43 alignment of PduX homologues and other GHMP (galactokinase, homoserine kinase, mevalonate kinase, and

44 rally located motif, which characterizes the galactokinase/homoserine kinase/ mevalonate kinase/phosp

45 Originally, the gene encoding galactokinase in humans was mapped to chromosome 17.

46 Galactokinase is a single copy gene in Arabidopsis, whic

47 onal, as it could complement a S. cerevisiae galactokinase mutant.

48 sed on these findings, orphan members of the galactokinase, nucleoside monophosphate kinase, and pyro

49 data indicate that the sequence reported for galactokinase on chromosome 15 is that of GalNAc kinase,

50 orms of the disorder can occur due to either galactokinase or UDP-galactose 4-epimerase deficiencies.

51 the Gal4p inhibitory protein Gal80p and the galactokinase paralog, Gal3p.

52 The purified galactokinases phosphorylated galactose, but not GalNAc,

53 Galactokinase plays a key role in normal galactose metab

54 Galactokinase plays a key role in normal galactose metab

55 ids showed 90% identity to sequence of human galactokinase reported to be on chromosome 15.

56 utarotase also participates by producing the galactokinase substrate alpha-D-galactose from its beta-

57 omevalonate decarboxylase and kinases of the galactokinase superfamily, between the metazoan phosphom

58 As a result of a higher affinity of galactokinase toward galactose, the lumped constant (LC)

59 A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the cours

60 coding a protein with sequence similarity to galactokinase, was subsequently mapped to chromosome 15.

61 ine, the specificity, and the optimal pH for galactokinase were studied.

62 sfected with the cDNA from presumptive human galactokinase, were compared.

63 mutations have now been identified in human galactokinase, which result in the diseased state referr

64 the three-dimensional architecture of human galactokinase with bound alpha-D-galactose and Mg-AMPPNP

65 is conveyed by a cytoplasmically transmitted galactokinase working as a signal transducer.