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1 of X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside).
2 l alpha-D-glucopyranoside and methyl alpha-D-galactopyranoside.
3 feature of a 4,6 cyclic phosphate on a beta-galactopyranoside.
4 ant Glu126-->Asp binds p-nitrophenyl-alpha,D-galactopyranoside.
5 e and enzyme assay with o-nitrophenyl beta-D-galactopyranoside.
6 rophenyl fucopyranoside versus p-nitrophenyl galactopyranoside.
7 ve towards the substrate p-nitrophenyl alpha-galactopyranoside.
8 luorescent substrate C12-fluorescein di-beta-galactopyranoside.
9 - 0.1 that is insensitive to the presence of galactopyranoside.
10 t but the mutants retain the ability to bind galactopyranoside.
11 at O-3 were prepared from p-methoxyphenyl d-galactopyranoside.
12 between protonation and binding of the cargo galactopyranoside.
13 than the WT enzyme on p-nitrophenyl alpha-d-galactopyranoside.
14 opyranoside and 4-nitrophenyl-2-deoxy-beta-d-galactopyranoside.
15 nten constant (KM) of 1.2 mM for the indolyl galactopyranoside.
16 in and a polar moiety, including the alpha-d-galactopyranoside.
17 binopyranoside but also p-nitrophenyl beta-D-galactopyranoside.
18 to discriminate primarily between gluco- and galactopyranosides.
19 harvested 3.5 h post-isopropyl-1-thio-beta-D-galactopyranoside (0.4 mM)-induction growth at 25 degree
20 2-propyl 2,6-di-O-acetyl-3,4-epithio-alpha-D-galactopyranoside (14) gave the derivative of Glcp-beta-
21 26-O-alpha-l-rhamnopyranosyl (1-->2)-beta-d-galactopyranoside (3a,3b), furosta-2alpha,3beta,22xi,26-
22 e competitive bioassay, 4-aminophenyl beta-d-galactopyranoside (4-APG) conjugated to QDs competes wit
23 ha-D-galactopyranosyl-(6'' --> 1''')-alpha-d-galactopyranoside (5) and benzoyl-O-alpha-D-glucopyranos
26 1), and alpha-O-benzyl 2-acetamido-2-deoxy-D-galactopyranoside (8), confirming the critical requireme
27 tes, a colorimetric (chlorophenol red-beta-D-galactopyranoside), a chemiluminescent (Lumi-Gal 530), a
28 of beta,D-galactosylpyranosyl 1-thio-beta,D-galactopyranoside, a property observed with Glu269-->Asp
29 on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) agar plates containing uracil and uri
31 -262-->Trp) with bound p-nitrophenyl-alpha-d-galactopyranoside (alpha-NPG), a high-affinity lactose a
32 asts with benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside, an inhibitor of O-linked glycosylatio
33 se for cleavage of both 4-nitrophenyl-beta-d-galactopyranoside and 4-nitrophenyl-2-deoxy-beta-d-galac
34 rophenyl fucopyranoside versus o-nitrophenyl galactopyranoside and a 300-fold increased substrate spe
35 f the fluorogenic substrate resorufin beta-d-galactopyranoside and a constant amount of the enzyme be
36 he coupled stoichiometric translocation of a galactopyranoside and an H(+) across the cytoplasmic mem
37 mic membrane protein, catalyzes symport of a galactopyranoside and an H(+) by using an alternating ac
38 nce of 2'-(N-dansyl)aminoalkyl-1-thio-beta-D-galactopyranoside and Na(+), Li(+), or H(+), which impli
39 low and high salt buffers, of methyl beta-d-galactopyranoside and the methyl glycoside of beta(1-->6
40 sidue in place of A122 (helix IV) transports galactopyranosides and is specifically inactivated by me
41 rate of acid-catalyzed hydrolysis of methyl galactopyranosides and of spontaneous hydrolysis of 2,4-
42 tosidase and its substrate (resorufin-beta-D-galactopyranoside) and found K(m) and k(cat) to be 333 +
43 xidation of 1-O-methyl-6, 6'-di-[2H]-alpha-d-galactopyranoside, and at high galactose concentrations,
45 -consumption assays using 1-O-methyl-alpha-D-galactopyranoside as the sugar substrate to produce an a
47 tosidase and its substrate, resorufin-beta-D-galactopyranoside, as the model system of the enzyme rea
48 re identified as cholesteryl 6-O-acyl-beta-d-galactopyranoside (B. burgdorferi glycolipid 1, BbGL-I),
49 Using benzyl 2-acetamido-2-deoxy-alpha-d-galactopyranoside (BADG) to inhibit complete O-linked gl
50 beta(alpha)-d-galactopyranosyl-(1,1)-beta-d-galactopyranoside (betabeta-(Galp)(2)) and alphabeta-(Ga
52 Nitro- or methyl-substituted phenyl alpha-D-galactopyranosides bind with significantly higher affini
53 Surprisingly, relative to methyl alpha-D-galactopyranoside, binding of cyclohexyl alpha-D-galacto
57 anoside to give 4-nitrophenyl-2-deoxy-beta-d-galactopyranoside causes (1) a change in the rate-determ
58 3 fusion protein was isopropyl-1-thio-beta-D-galactopyranoside concentration-, induction growth time-
59 action resulted in formation of azide beta-d-galactopyranoside, confirming Asp327 as the nucleophilic
61 orimetric assay with chlorophenol red-beta-d-galactopyranoside (CPRG) for bacteria quantification.
62 the color substrate, chorophenol red-beta-D-galactopyranoside (CPRG), the signal from as few as 400
63 l was detected using chlorophenol red-beta-d-galactopyranoside (CRPG), a colorimetric substrate which
64 hloro-9,9-dimethylacridin-2-one-7-yl) beta-d-galactopyranoside (DDAOG), can be cleaved by beta-gal to
65 sugar (2'-(N-dansyl)aminoalkyl-1-thio-beta-D-galactopyranoside) demonstrate that MelB-ST, in the pres
66 the binding 1-beta carbohydrate-substituted galactopyranoside derivatives to galectin-1 from bovine
67 e, 6, 8-difluoro-4-methylumbelliferyl beta-d-galactopyranoside (DiFMUG), was found to be considerably
70 iously, methyl- or allyl-substituted alpha-D-galactopyranosides exhibit a 60-fold increase in binding
71 nchanged (K(D) = 0.4 mM), and phenyl alpha-D-galactopyranoside exhibits only a modest increase in bin
72 atalyzed hydrolysis of fluorescein di-beta-D-galactopyranoside (FDG), a commonly used fluorescent sub
75 lected, induced with isopropyl-1-thio-beta-D-galactopyranoside for fusion gene expression and an expr
76 d hydrolysis of 4-nitrophenyl-2-deoxy-beta-d-galactopyranoside from breakdown to formation of the cov
77 spontaneous hydrolysis of 2,4-dinitrophenyl galactopyranosides has been studied through the synthesi
80 ic hypoxic markers beta-D-iodinated azomycin galactopyranoside (IAZGP) and [99mTc]HL-91 were simultan
81 luate iodine 124 (124I)-labeled iodoazomycin galactopyranoside (IAZGP) positron emission tomography (
83 observations that hydrophobic aglycons of D-galactopyranosides increase binding affinity, with a cle
86 ition, beta,D-galactopyranosyl 1-thio-beta,D-galactopyranoside induces a significant increase in cros
87 ed to stably express isopropyl-1-thio-beta-D-galactopyranoside-inducible Cox-2 (PCXII-4), we have inv
89 ignaling, we used an isopropyl-1-thio-beta-d-galactopyranoside-inducible expression system to express
91 ine lactones (AHLs) or isopropyl-beta-D-thio-galactopyranoside (IPTG) can be utilized for the impleme
92 the addition of 5 mM isopropyl-1-thio-beta-D-galactopyranoside (IPTG) induced the expression of Ha-Ra
95 or hydrolysis of 2,2,2-trifluoroethyl beta-D-galactopyranoside is due entirely to a wild type enzyme
96 nding specificity in lactose permease toward galactopyranosides is governed by H-bonding interactions
97 e model and indicate that specificity toward galactopyranosides is governed by H-bonding interactions
98 possesses similar affinities for methyl beta-galactopyranoside, lactose, and N-acetyllactosamine and
100 cifically inactivated by methanethiosulfonyl-galactopyranosides (MTS-gal), which behave as unique sui
101 y used substrate 4-methylumbelliferyl beta-d-galactopyranoside (MUG), for the detection of beta-galac
102 valently bound inactivator suggests that the galactopyranoside must be fully ligated to induce an occ
103 hloro-9,9-dimethylacridin-2-one-7-yl} beta-D-galactopyranoside), noninvasive in vivo imaging of apopt
104 tructural analogues of p-nitrophenyl alpha-D-galactopyranoside (NPG) was examined by site-directed N-
105 cetylated octadecyl 1-thio-alpha- and beta-D-galactopyranosides observed by TEM could be rationalized
106 ose or beta-d-galactopyranosyl 1-thio-beta-d-galactopyranoside of approximately 1.0 mM or 40 microM,
107 The substrate 2-fluoro-4-nitrophenyl beta-D-galactopyranoside (OFPNPG) is readily hydrolyzed by beta
108 e analyzed the entry of o-nitrophenyl-beta-D-galactopyranoside (ONPG) into cells induced to express V
109 s 2.8 and it hydrolysed o-nitrophenyl beta-d galactopyranoside (ONPG) with a K(m) value of 1.73 mM at
110 colorimetric substrate o-nitrophenyl-beta-d-galactopyranoside (ONPG), the signal-to-background ratio
111 The withdrawal of isopropyl-1-thio-beta-d-galactopyranoside or the addition of an inhibitor of the
112 ith X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) or by immunofluorescence with an anti
116 that the C-2, C-3, C-4, and C-6 OH groups of galactopyranosides participate in important H-bonding in
117 1.5+/-2.7% 5-bromo-4-chromo-3-indolyl-beta-D-galactopyranoside-positive cells and 81.1+/-3.6 U/g beta
118 eta-d-idopyranosyl configuration from beta-d-galactopyranosides, prior to a C-6-homologation extendin
119 analog beta-D-galactopyranosyl 1-thio-beta-D-galactopyranoside protects both lactose transport and en
120 mease, beta-D-galactopyranosyl 10thio-beta-D-galactopyranoside quenches the fluorescence of 2-(4'-mal
123 a-L-arabinopyranose residues than for beta-D-galactopyranoside residues and possessed a novel enzymat
125 molog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the
127 Kinetic studies using o-nitrophenyl-beta-D-galactopyranoside showed that BgaS with and without a Hi
128 We conclude that protonated LacY binds D-galactopyranosides specifically, inducing an occluded st
129 ermined by 5-bromo-4-chromo-3-indolyl-beta-D-galactopyranoside staining and beta-galactosidase assay
130 etected by 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside staining for Lac Z expressed by the vi
131 strated by 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside staining of cells infected by beta-gal
134 cal X-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) staining demonstrated expression of b
136 ein, and the side chains shown to ligate the galactopyranoside strongly confirm more than two decades
138 ity with the "native"para-nitrophenyl-beta-d-galactopyranoside substrate) were evolved in seven round
139 ining with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside substrate, indicating expression of th
140 c myocytes using lipophilic fluorogenic beta-galactopyranoside substrates for real-time detection of
141 nce of beta-D-galactopyranosyl 1-thio-beta-D-galactopyranoside (TDG) or a proton electrochemical grad
142 ion of beta-d-galactopyranosyl 1-thio-beta-d-galactopyranoside (TDG), indicating that ligand induces
143 nce of beta-D-galactopyranosyl 1-thio-beta-D-galactopyranoside (TDG), lactose, or galactose at variou
145 ch those of beta-D-(125)I-iodinated azomycin galactopyranoside, the optimal hypoxia marker of the azo
146 lyzes the hydrolysis of 4-nitrophenyl beta-D-galactopyranoside through a galactosyl-enzyme intermedia
147 ning of this carbon atom in alpha- or beta-D-galactopyranosides thus may provide a rationale for the
148 C2-OH group by C2-H at 4-nitrophenyl-beta-d-galactopyranoside to give 4-nitrophenyl-2-deoxy-beta-d-g
149 ctopyranoside, binding of cyclohexyl alpha-D-galactopyranoside to lactose permease is essentially unc
151 reaction of nonfluorescent resorufin-beta-D-galactopyranoside to yield D-galactose and fluorescent r
154 ccumulation of proline and thiomethyl-beta-D-galactopyranoside was partially restored to wild-type le
156 L-arabinopyranoside and p-nitrophenyl beta-D-galactopyranoside were compared, the K(m) values were 1.
157 vatives of beta-D glucopyranoside and beta-D-galactopyranoside were prepared by direct sulfation of t
159 p21(WAF1) induced by isopropyl-1-thio-beta-D-galactopyranoside were similar to those induced by NGF.
160 2-deoxy-3-O-beta-D-galactopyranosyl-alpha- D-galactopyranosides, were found to be effective chromogen
161 -2-deoxy-3-O-beta -D-galactopyranosyl-beta-D-galactopyranoside, which is a competitive inhibitor of t
166 bioluminogenic substrate, D-luciferin-O-beta-galactopyranoside, with short assay time and small volum
167 irst stained with 5-bromo-4-chloro-3-indolyl galactopyranoside (X-gal) as wholemounts and then sectio
168 substrate 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal) at pH 6.0, a reaction conditio
170 tochemical 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal) staining demonstrated amelobla
172 lyzed with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) to monitor beta-galactosidase
173 ented with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) to monitor lacZ gene expressio
174 ining with 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-gal), a quantitative spectrophotome
175 ining with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal), and the frequencies of inacti
176 ibution of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal)-stained cells was examined in
178 mination of 5bromo-4-chloro-3-indolyl-beta-D-galactopyranoside- (X-gal) and halogenated indolyl-beta-
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