ライフサイエンスコーパス: gamma-tubulin

1 x (gamma-TuC), consisting of GCP1-6 (GCP1 is gamma-tubulin).

2 CPAP) and the pericentriolar localization of gamma-tubulin.

3 d dendrites, we examined the distribution of gamma-tubulin.

4 in-1 (BAP1) as a deubiquitination enzyme for gamma-tubulin.

5 play altered fractionation of beta-actin and gamma-tubulin.

6 tive complex and likely equimolar amounts of gamma-tubulin.

7 organizing centers with Aurora kinase A and gamma-tubulin.

8 taining gain- or loss-of-function alleles of gamma-tubulin.

9 levels of the microtubule nucleation protein gamma-tubulin.

10 by promoting the centrosomal accumulation of gamma-tubulin.

11 to the centrosome independently of BARD1 and gamma-tubulin.

12 d that both arrestins directly interact with gamma-tubulin.

13 (MTOC)-associated proteins, pericentrin and gamma-tubulin.

14 mains mediate longitudinal interactions with gamma-tubulin.

15 GIT1 and gamma-tubulin complex proteins with gamma-tubulin.

16 sequence" present in all alpha-, beta-, and gamma-tubulins.

17 lts in coprecipitation of alpha-, beta-, and gamma-tubulins.

18 e levels are not changed by codepleting both gamma-tubulins.

19 was not further prolonged when we codepleted gamma-tubulins.

20 y originate in the C-terminal regions of the gamma-tubulins.

21 ata indicate that in the face of predominant gamma-tubulin-1 expression, the accumulation of gamma-tu

22 Localization of gamma-tubulin-1 in mature neurons was confirmed by immun

23 ve RT-PCR and 2-dimensional-PAGE showed that gamma-tubulin-1 is the dominant isotype in fetal neurons

24 ma-tubulin-2 accumulates in the adult brain, gamma-tubulin-1 remains the major isotype in various bra

25 It is thought that gamma-tubulin-1 represents a ubiquitous isotype, whereas

26 f gamma-tubulin-2, whereas the expression of gamma-tubulin-1 was unchanged.

27 Although gamma-tubulin-2 accumulates in the adult brain, gamma-tu

28 ma-tubulin-1 expression, the accumulation of gamma-tubulin-2 in mature neurons and neuroblastoma cell

29 tive stress may denote a prosurvival role of gamma-tubulin-2 in neurons.

30 n-1 represents a ubiquitous isotype, whereas gamma-tubulin-2 is found predominantly in the brain, whe

31 l development and oxidative stress points to gamma-tubulin-2 prosurvival function.

32 rial inhibitors, resulted in upregulation of gamma-tubulin-2, whereas the expression of gamma-tubulin

33 MIN subunit3 (AUG3), a homolog of animal dim gamma-tubulin 3, plays a critical role in gamma-tubulin-

34 AUG3), which encodes a homolog of animal dim gamma-tubulin 3/human augmin-like complex, subunit 3, wa

35 nistically, NDRG1 physically associates with gamma-tubulin, a key component of the centrosome, with r

36 We report discrimination of human gamma-tubulins according to their electrophoretic and im

37 ntially decreases microtubule nucleation and gamma-tubulin accumulation at the centrosome.

38 n and Src promote microtubule nucleation and gamma-tubulin accumulation at the centrosome.

39 respective epitope showing immunolabeling of gamma-tubulin, actin, Golgi protein, and the transcripti

40 complex subunit in this tissue revealed that gamma-tubulin acts with NOCA-1 in parallel to Patronin/P

41 urgery altered the trafficking of alpha- and gamma-tubulin after fertilization.

42 A cold-sensitive gamma-tubulin allele of Aspergillus nidulans, mipAD159,

43 Nuclear gamma-tubulin also regulates S-phase progression by mode

44 eated from multiprotein complexes containing gamma-tubulin and associated gamma-tubulin complex prote

45 nd spindle formation rely on the activity of gamma-tubulin and associated proteins throughout the cel

46 Branching microtubule nucleation requires gamma-tubulin and augmin and is stimulated by factors pr

47 that microtubule-nucleating proteins such as gamma-tubulin and CeGrip-1 that are centrosome component

48 s apically and have aberrant localization of gamma-tubulin and CeGrip-1.

49 We showed that MPK6 interacted with gamma-tubulin and co-sedimented with plant microtubules

50 This acentrosomal nucleation requires gamma-tubulin and CP309, the Drosophila homolog of AKAP4

51 ediated a novel interaction between p115 and gamma-tubulin and functioned in its centrosomal targetin

52 is mediated by complexes that are formed by gamma-tubulin and gamma-tubulin complex proteins.

53 ARH interacts with centrosomal (gamma-tubulin and GPC2 and GPC3) and motor (dynein heavy

54 Pk3 activity, cilia growth was inhibited and gamma-tubulin and Nedd1 no longer associated with the ba

55 We show that the microtubule regulators gamma-tubulin and NOCA-1 are recruited to hemidesmosomes

56 (gamma-TuSC), consisting of two molecules of gamma-tubulin and one copy each of the accessory protein

57 ex (gamma-TuSC) consists of two molecules of gamma-tubulin and one molecule each of Spc97 and Spc98.

58 NEDD1 specifically co-localizes with gamma-tubulin and pericentrin at microtubule-organizing

59 Moreover, both gamma-tubulin and pericentrin expression at MTOCs were d

60 iated with the MT-organizing center proteins gamma-tubulin and pericentrin, are major sites of muscle

61 on allele impairs centrosomal recruitment of gamma-tubulin and pericentrin, interferes with microtubu

62 tic mutant of BAP1 reduced ubiquitination of gamma-tubulin and prevented mitotic defects.

63 correlation between the expression levels of gamma-tubulin and RB1 and that in tumor cell lines with

64 so defective in spindle pole localization of gamma-tubulin and showed spindle assembly checkpoint (SA

65 crotubules are stabilized by the presence of gamma-tubulin and Spc72, a protein that tethers the gamm

66 role in mitotic spindle positioning through gamma-tubulin and spindle fidelity through an unknown me

67 gulation of Ran GTPase, serotransferrin, and gamma-tubulin and suppression of light-evoked electrophy

68 olar and pericentriolar components including gamma-tubulin and the centriole duplication factors ZYG-

69 inases regulate the cellular localization of gamma-tubulin and thereby control S-phase progression.

70 -tubulin complex in T. brucei is composed of gamma-tubulin and three GCP proteins, GCP2-GCP4, and is

71 ose gradients and that XCTK2 associated with gamma-tubulin and Xgrip109 by immunoprecipitation.

72 osed nucleation/stabilization factors, TPX2, gamma-tubulin and XMAP215, in chromatin-promoted assembl

73 been made in understanding the functions of gamma-tubulin and, in particular, its role in microtubul

74 n of mature centrioles capable of recruiting gamma-Tubulin, and a nonphosphorylatable Cetn2 mutant ca

75 ry centrosomes had less centrosome-localized gamma-tubulin, and Plk1 blockade prevented MT growth, wh

76 ary basal bodies, physically interacted with gamma-tubulin, and was present along ciliary axonemes, s

77 nt for proper recruitment of pericentrin and gamma-tubulin, and, ultimately, for formation of normal

78 First, gamma-tubulin appears to redistribute directly from the

79 Alpha-, beta-, and gamma-tubulin are conserved in all eukaryotes.

80 gamma-Tubulins are highly conserved members of the tubul

81 N GTPase, MST1 and 2 kinases, and alpha- and gamma-tubulin as RASSF1A-interacting proteins.

82 We discovered that Arpc1b colocalizes with gamma-tubulin at centrosomes and stimulates Aurora A act

83 ndle stability was associated with decreased gamma-tubulin at MTOCs in NEDD1-depleted oocytes, as wel

84 hat codepletion of MYPT1 and PLK1 reinstates gamma-tubulin at the centrosomes, rescuing the mitotic a

85 Here we use it to analyze gamma-tubulin binding to the mitotic spindle and centros

86 beta-tubulin, the 2.3 A crystal structure of gamma-tubulin bound to GDP, and kinetic simulations to i

87 lexes (gammaTuSCs) comprise two molecules of gamma-tubulin bound to the C-terminal domains of GCP2 an

88 xpression of a non-phosphorylatable Ala(385)-gamma-tubulin but were enhanced by expression of SadB, w

89 the BRCA1/BARD1-dependent ubiquitination of gamma-tubulin causes centrosome amplification.

90 Here we report that GRK5 co-localizes with gamma-tubulin, centrin, and pericentrin in centrosomes.

91 ow that the localization of the MT nucleator gamma-tubulin changes from diffuse cytoplasmic staining

92 In the germline, NOCA-1 and gamma-tubulin co-localize at the cell surface, and inhib

93 RacGAP50C and gamma-tubulin colocalize at perinuclear sites in myotube

94 hich forms a complex with GCP2-GCP6 (GCP for gamma -Tubulin Complex Protein).

95 through recruiting two critical factors, the gamma-tubulin complex (gamma-TuC) and polo kinase (Plo1)

96 The multisubunit gamma-tubulin complex (gamma-TuC) is critical for microt

97 nctions as a multiprotein complex called the gamma-tubulin complex (gamma-TuC), consisting of GCP1-6

98 to1/2 complex, which binds and activates the gamma-tubulin complex and also recruits the gamma-tubuli

99 o1/2 complex stability, interaction with the gamma-tubulin complex and microtubule nucleation activit

100 FAM29A interacts with the NEDD1-gamma-tubulin complex and recruits this complex to the s

101 suggest that Spc110 facilitates higher-order gamma-tubulin complex assembly.

102 gamma-Tubulin complex constitutes a key component of the

103 Here we report that the gamma-tubulin complex in T. brucei is composed of gamma-

104 ogether, these results identified an unusual gamma-tubulin complex in T. brucei, uncovered an essenti

105 ytokinesis defects, suggesting a role of the gamma-tubulin complex in the regulation of cytokinesis.

106 To date, it has been enigmatic how the gamma-tubulin complex is recruited to the sidewall of co

107 show that during mitosis GIPs play a role in gamma-tubulin complex localization, spindle stability an

108 To date, the molecular mechanisms modulating gamma-tubulin complex location remain largely unknown.

109 Microtubule nucleation by the gamma-tubulin complex occurs primarily at centrosomes, b

110 The conserved gamma-tubulin complex organizes spindle and astral micro

111 mplex (gammaTuSC) composed of gamma-tubulin, gamma-tubulin complex protein (GCP)2 and GCP3, whereas a

112 plex with Alp6, a fission yeast homologue of gamma-tubulin complex protein 3 (GCP3).

113 investigate the role of the highly conserved gamma-tubulin complex protein 3-interacting proteins (GI

114 TUBGCP4 encodes gamma-tubulin complex protein 4, a component belonging t

115 ocations marked by green fluorescent protein-gamma-tubulin complex protein2-tagged gamma-nucleation c

116 distribution pattern, similar to that of the gamma-tubulin complex protein2.

117 abidopsis thaliana proteins interacting with gamma-tubulin complex protein3 (GCP3), GCP3-interacting

118 le from those of the AUG1-7 subunits and the gamma-tubulin complex proteins (GCPs) that exhibit biase

119 exes containing gamma-tubulin and associated gamma-tubulin complex proteins (GCPs).

120 s and stimulates the association of GIT1 and gamma-tubulin complex proteins with gamma-tubulin.

121 mplexes that are formed by gamma-tubulin and gamma-tubulin complex proteins.

122 Budding yeast Spc110, a member of gamma-tubulin complex receptor family (gamma-TuCR), recr

123 amma-tubulin small complex (gamma-TuSC), and gamma-tubulin complex receptors (gamma-TuCRs) Spc72 and

124 TOC sites has been proposed as a key step in gamma-tubulin complex recruitment and MTOC formation, bu

125 n Trypanosoma brucei, the composition of the gamma-tubulin complex remains elusive, and it is not kno

126 Controlled degradation of a gamma-tubulin complex subunit in this tissue revealed th

127 ns of MOZART1/Mzt1 through interactions with gamma-tubulin complex subunits and gamma-TuCRs.

128 ) as an additional member of the BRCA1/BARD1/gamma-tubulin complex that is critically involved in cen

129 gamma-tubulin complex and also recruits the gamma-tubulin complex to both centrosomal (spindle pole

130 e augmin complex functions in recruiting the gamma-tubulin complex to cortical MTs and initiating MT

131 T)-dependent MT nucleation by recruiting the gamma-tubulin complex to MT walls to generate new MTs [1

132 ubulin and Spc72, a protein that tethers the gamma-tubulin complex to the spindle pole body.

133 complex overwhelmingly colocalized with the gamma-tubulin complex.

134 confirmed this finding for Spc72 and for the gamma-tubulin complex.

135 ch localizes to MTOCs and interacts with the gamma-tubulin complex.

136 nown to involve the evolutionarily conserved gamma-tubulin complex.

137 DD1/GCP-WD protein, which interacts with the gamma-tubulin complex.

138 its function is likely linked to that of the gamma-tubulin complex.

139 gamma-Tubulin complexes are essential for microtubule (M

140 In contrast, Nedd1-gamma-tubulin complexes did not promote nucleation but w

141 n has been determined, and the components of gamma-tubulin complexes have been identified.

142 Although gamma-tubulin complexes have primarily been implicated i

143 The probability of nucleation from gamma-tubulin complexes localized at the cell cortex was

144 ively, our studies demonstrate that distinct gamma-tubulin complexes regulate different microtubule b

145 ulin on Ser(385) formed chromatin-associated gamma-tubulin complexes that moderate gene expression.

146 mplex receptor family (gamma-TuCR), recruits gamma-tubulin complexes to microtubule (MT) organizing c

147 RT1/Mzt1 is required for the localization of gamma-tubulin complexes to microtubule (MT)-organizing c

148 Nucleation-competent CDK5RAP2-gamma-tubulin complexes were maintained at centrosomes u

149 le exit specifically triggered loss of Nedd1-gamma-tubulin complexes, providing a mechanistic link co

150 Microtubules are nucleated in vivo by gamma-tubulin complexes.

151 ssion, therefore, precedes the appearance of gamma-tubulin-containing centrosomes.

152 The bulk of CMTs are initiated from gamma-tubulin-containing nucleation complexes localized

153 by the composition, position and dynamics of gamma-tubulin-containing nucleation complexes, which rep

154 ogue of the APC/C activator protein Cdh1, in gamma-tubulin-dependent inactivation of the APC/C.

155 Ts can be generated from preexiting MTs in a gamma-tubulin-dependent manner in yeast, plant, and Dros

156 im gamma-tubulin 3, plays a critical role in gamma-tubulin-dependent MT nucleation and amplification

157 l proteins, including Pericentrin, Pcm1, and gamma-tubulin, depends on Nesprin-1, an outer nuclear me

158 -body components, provokes misrecruitment of gamma-tubulin, disorganization of this microtubule frame

159 red for normal spindle pole organization and gamma-tubulin distribution.

160 ins co-localized with the centrosomal marker gamma-tubulin during interphase and mitosis and were fou

161 Reducing levels of gamma-tubulin exacerbated long-term degeneration induced

162 at gamma-tubulin mutations or alterations of gamma-tubulin expression play an important role in certa

163 Second, gamma-tubulin fails to accumulate apically in wild-type

164 Moreover, in activated BMMCs, gamma-tubulin formed complexes with tyrosine-phosphoryla

165 c siRNA injection caused the dissociation of gamma-tubulin from the spindle poles, resulting in sever

166 gamma-Tubulin functions as a multiprotein complex called

167 ce recovery kinetics we observe implies that gamma-tubulin functions by binding weakly to spindle mic

168 Thus, gamma-tubulin functions to regulate this key mitotic and

169 ubulin small complex (gammaTuSC) composed of gamma-tubulin, gamma-tubulin complex protein (GCP)2 and

170 fusions have shown that GIPs colocalize with gamma-tubulin, GCP3, and/or GCP4 and reorganize from the

171 Humans possess 2 gamma-tubulin genes.

172 enters (MTOCs) and coimmunoprecipitates with gamma-tubulin Gtb1 from cell extracts.

173 These results (with our previous gamma-tubulin:GTPgammaS structure) support the lattice m

174 The structure of gamma-tubulin has been determined, and the components of

175 At the same time, data have accumulated that gamma-tubulin has important but less well understood fun

176 ubules and interacts with alpha-, beta-, and gamma-tubulin, heat shock proteins 70 and 90 (HSP-70; HS

177 indispensable component for the function of gamma -tubulin in MT nucleation and organization in plan

178 We found that GCP4 was associated with gamma -tubulin in vivo in Arabidopsis thaliana.

179 In our efforts to understand the role of gamma-tubulin in cell cycle regulation, we have created

180 dle and centrosomes to determine the role of gamma-tubulin in microtubule nucleation in the spindle.

181 The aug7-1 mutation caused delocalization of gamma-tubulin in the mitotic spindle and phragmoplast.

182 etion spread its localization beyond that of gamma-tubulin, indicating an MT-dependent regulation of

183 , in bone marrow-derived mast cells (BMMCs), gamma-tubulin interacts with p21-activated kinase intera

184 gamma-Tubulin is an important cell division regulator th

185 The diffusion coefficient of gamma-tubulin is consistent with a major species existin

186 evolutionarily conserved Cdk1 site (S360) in gamma-tubulin is correlated with the number and organiza

187 The mechanism that regulates localization of gamma-tubulin is currently unknown.

188 Moreover, we show that binding to gamma-tubulin is not essential for integrating into the

189 Microtubule (MT)-dependent MT nucleation by gamma-tubulin is required for interphase plant cells to

190 the purported functional differences between gamma-tubulins is unknown.

191 Differential expression of human gamma-tubulin isotypes during neuronal development and o

192 tibodies that can discriminate between human gamma-tubulin isotypes.

193 se each gamma-TuSC contains two molecules of gamma-tubulin, it was assumed that the gamma-TuRC-specif

194 h, whereas overexpression rescued centrosome gamma-tubulin levels and centrosome dynamics.

195 vels, with actin levels increased and alpha-/gamma-tubulin levels reduced.

196 we show that loss of B1 enhanced centrosomal gamma-tubulin localization and microtubule nucleation.

197 We examined gamma-tubulin localization and microtubule regrowth afte

198 gamma-Tubulin localizes to the plasma membrane in additi

199 To explore whether microtubule nucleation by gamma-tubulin might contribute to polarity, we analyzed

200 , gamma-TuSCs oligomerize into spirals of 13 gamma-tubulin molecules per turn.

201 anchoring of MTs required the same number of gamma-tubulin molecules.

202 ma-TuSCs with approximately three additional gamma-tubulin molecules.

203 icrotubules and followed similar dynamics to gamma-tubulin, moving from poles to midzone during the a

204 gamma-tubulin, or a phosphomimetic Asp(385)-gamma-tubulin mutant.

205 A gamma-tubulin mutation in Aspergillus nidulans, mipA-D15

206 Finally, the gamma-tubulin mutation mipAD159 causes a nuclear-specifi

207 Finally, evidence is emerging that gamma-tubulin mutations or alterations of gamma-tubulin

208 These centrioles can neither recruit gamma-tubulin nor nucleate microtubules when eggs are in

209 Cytosolic gamma-tubulin nucleates alpha- and beta-tubulin in a gro

210 r that polymerizes to form microtubules, and gamma-tubulin nucleates microtubules as a component of t

211 f seven gamma-TuSCs with a slight surplus of gamma-tubulin nucleates MTs in vivo.

212 We show that the gamma-tubulin nucleation complex (gammaTC) favors the ol

213 found that SadB-mediated phosphorylation of gamma-tubulin on Ser(385) formed chromatin-associated ga

214 the active form of MAP kinase interacts with gamma-tubulin on specific subsets of mitotic microtubule

215 , compromises the localization of augmin and gamma-tubulin on the spindle and phragmoplast MT arrays

216 Depletion of either gamma-tubulin or XMAP215 was partially rescued by adding

217 re enhanced by expression of SadB, wild-type gamma-tubulin, or a phosphomimetic Asp(385)-gamma-tubuli

218 ic reconstruction of the filament reveals 13 gamma-tubulins per turn, matching microtubule symmetry,

219 ion led to loss of PCM components, including gamma-tubulin, pericentrin, and Cdk5Rap2, with centrosom

220 pericentriolar proteins to MTNCs, including gamma-tubulin, pericentrin, Cep68, Cep170, and Cdk5RAP2.

221 gamma-Tubulin plays a universal role in microtubule nucl

222 on to the spindle pole body, and, thus, that gamma-tubulin plays an important role in inactivating AP

223 anced level of free cytosolic Ca(2+) affects gamma-tubulin properties and stimulates the association

224 ines with a nonfunctioning RB1, reduction of gamma-tubulin protein levels leads to induction of apopt

225 Simultaneous reduction of RB1 and gamma-tubulin protein levels results in an E2F1-dependen

226 Here, we describe that RB1 and gamma-tubulin proteins moderate each other's expression

227 g gamma-tubulin small complex (gammaTuSC) or gamma-tubulin, rather than gamma-tubulin ring complex (g

228 Binding of the gamma-tubulin receptor Spc110 to the central plaque from

229 1 binds to the scaffold-protein Nud1 and the gamma-tubulin receptor Spc72.

230 aphase and anaphase, leading to disorganized gamma-tubulin recruitment in centrosomes.

231 terfering RNAs is known to result in loss of gamma-tubulin recruitment to the centrosomes, blocking c

232 impaired both the Augmin-MT interaction and gamma-tubulin recruitment to the spindles, thus resultin

233 ts in mislocalized Plk1 and poor centrosomal gamma-tubulin recruitment, potentially contributing to m

234 In this way, the C-terminal region of gamma-tubulin regulates S-phase progression.

235 out of dendrites with an activated kinesin, gamma-tubulin remained in dendrites.

236 sites in myotubes, and in RacGAP50C mutants gamma-tubulin remains dispersed throughout the cytoplasm

237 demonstrates that Pontin interacts with the gamma tubulin ring complex (gamma-TuRC).

238 plex protein 4, a component belonging to the gamma-tubulin ring complex (gamma-TuRC) and known to reg

239 ein-dependent manner and interacted with the gamma-tubulin ring complex (gamma-TuRC) and the centriol

240 iates interactions with proteins of both the gamma-tubulin ring complex (gamma-TuRC) and the gamma-tu

241 fission yeast, the kinesin-14 Pkl1 binds the gamma-tubulin ring complex (gamma-TuRC) microtubule-orga

242 ted that Cdc2-dependent phosphorylation on a gamma-tubulin ring complex (gamma-TuRC) recruitment prot

243 chondrial surface, recruits the MT nucleator gamma-tubulin ring complex (gamma-TuRC), and is sufficie

244 requires many identified components, such as gamma-tubulin ring complex (gamma-TuRC), components of t

245 crotubules (MTs), which are nucleated by the gamma-tubulin ring complex (gamma-TuRC).

246 (GCP)2 and GCP3, whereas animals contain the gamma-tubulin ring complex (gammaTuRC) composed of gamma

247 In vivo, MT nucleation is controlled by the gamma-tubulin ring complex (gammaTuRC), a 2.1-MDa comple

248 ex (gammaTuSC) or gamma-tubulin, rather than gamma-tubulin ring complex (gammaTuRC).

249 5, and 6) to form the core of the so-called gamma-tubulin ring complex (gammaTuRC).

250 letion in human cells destabilizes the large gamma-tubulin ring complex and abolishes CEP215(CM1)-ind

251 ogress in understanding the structure of the gamma-tubulin ring complex and its components has led to

252 omains leads to the loss of anchoring of the gamma-tubulin ring complex and of nucleation of microtub

253 ues to generate the attachment sites for the gamma-tubulin ring complex and, possibly, other pericent

254 me and functions in the stabilization of the gamma-tubulin ring complex assembly.

255 FAM190A was localized to the gamma-tubulin ring complex of early mitosis and to the m

256 protein2-tagged gamma-nucleation complexes (gamma-tubulin ring complex), therefore indicating that a

257 Microtubule nucleation requires the gamma-tubulin ring complex, and during the M-phase (mito

258 otic spindle formation as a component of the gamma-tubulin ring complex.

259 ed by a noncanonical mechanism not involving gamma-tubulin ring complex.

260 nucleates microtubules as a component of the gamma-tubulin ring complex.

261 tion of microtubules arises from distributed gamma-tubulin ring complexes (gamma-TuRCs) at the cell c

262 Microtubules are nucleated and anchored by gamma-tubulin ring complexes (gamma-TuRCs) embedded with

263 amma-TuSCs assemble with other proteins into gamma-tubulin ring complexes (gamma-TuRCs).

264 bule nucleation within cells is catalyzed by gamma-tubulin ring complexes localized at specific micro

265 ET and Xenopus XCTK2 cofractionated with the gamma-tubulin ring complexes on sucrose gradients and th

266 However, gamma-tubulin RNAi delays microtubule regrowth after dep

267 In mitotic cells, it was observed that the gamma -tubulin signal associated with the mitotic spindl

268 Thus, the RB1/gamma-tubulin signal network can be considered as a new

269 The gamma-tubulin small complex (gamma-TuSC) consists of two

270 , we reconstituted the interactions of Mzt1, gamma-tubulin small complex (gamma-TuSC), and gamma-tubu

271 The 300-kDa gamma-tubulin small complex (gamma-TuSC), consisting of

272 TUB4) encoding the evolutionarily conserved gamma-tubulin small complex (gamma-TuSC), which nucleate

273 ma-tubulin ring complex (gamma-TuRC) and the gamma-tubulin small complex (gamma-TuSC).

274 ere that budding yeast CK1delta, Hrr25, is a gamma-tubulin small complex (gammaTuSC) binding factor.

275 nt organisms: the budding yeast contains the gamma-tubulin small complex (gammaTuSC) composed of gamm

276 cytoplasm as the less efficiently nucleating gamma-tubulin small complex (gammaTuSC) or gamma-tubulin

277 x (gammaTuRC), a 2.1-MDa complex composed of gamma-tubulin small complex (gammaTuSC) subunits.

278 ltimers act to multimerize the fission yeast gamma-tubulin small complex and that multimerization of

279 ab11 increases astral microtubules, restores gamma-tubulin spindle pole localization, and generates r

280 persists as a cytoplasmic patch within which gamma-tubulin-stained centrosomes can be seen.

281 Shot/Patronin foci do not co-localize with gamma-tubulin, suggesting that they do not nucleate new

282 gamma-tubulin targets independently of NOCA-1, but NOCA-

283 ty in the epidermis to identify two pools of gamma-tubulin that are biochemically and functionally di

284 Both increased and decreased activity of gamma-tubulin, the core microtubule nucleation protein,

285 The posttranslational modification of gamma-tubulin through ubiquitination is vital for regula

286 lusory Clb3-Cdk1-specific phosphorylation of gamma-tubulin, thus establishing the timing of this even

287 These results show that NOCA-1 acts with gamma-tubulin to assemble non-centrosomal arrays in mult

288 A1-BARD1 heterodimer binds and ubiquitinates gamma-tubulin to inhibit centrosome amplification and pr

289 arotti mutants is sufficient to redistribute gamma-tubulin to the muscle fiber ends.

290 assembly regulators (i.e., AURKA, PLK1, and gamma-tubulin) to the microtubule-organizing center via

291 iolar material, specifically pericentrin and gamma-tubulin, to the centrosome.

292 y, mutation of one conserved Cdk site within gamma-tubulin (Tub4-S360D) caused mitotic delay and aber

293 pPKCdelta(Thr505) and pericentrin as well as gamma-tubulin was confirmed by co-immunoprecipitation an

294 recovery after photobleaching analysis that gamma-tubulin was stably integrated into MT nucleation s

295 In addition, chromatin levels of gamma-tubulin were decreased by the reduction of SadB le

296 with local nucleation, tagged and endogenous gamma-tubulins were found in specific positions in dendr

297 tly of NOCA-1, but NOCA-1 targeting requires gamma-tubulin when a non-essential putatively palmitoyla

298 pend on the evolutionarily conserved protein gamma -tubulin, which forms a complex with GCP2-GCP6 (GC

299 ism for BAP1 involved in deubiquitination of gamma-tubulin, which is required to prevent abnormal mit

300 ectron microscopy revealed an association of gamma-tubulins with mitochondrial membranes.