ライフサイエンスコーパス: gating current

1 ht beta 1A coexpressing cells had measurable gating currents).

2 hich leads to a "Boltzmann equation" for the gating current.

3 rged residues, and this movement generates a gating current.

4 process and thus the physical nature of the gating current.

5 ctivation process and thus the nature of the gating current.

6 reversal of toxin action and toxin block of gating current.

7 on by an applied electric field produces the gating current.

8 bsence of conductance and drastic changes in gating current.

9 d by previous work involving measurements of gating currents.

10 imentally with large-bandwidth recordings of gating currents.

11 from the VSD prevented the mode-shift of the gating currents.

12 vement of the voltage sensor, as measured by gating currents.

13 -dependence of the drug with macroscopic and gating currents.

14 and voltage dependence of ionic currents and gating currents.

15 and fast inactivation, we characterized HERG gating currents.

16 , the largest Q-V shift, and the fastest OFF gating currents.

17 ltage-clamped for measurements of Ca(2+) and gating currents.

18 ed mammalian cells, they both express robust gating currents.

19 ates off-gating currents without altering on-gating currents.

20 mately -40 mV as expected for sodium channel gating currents.

21 ocal anaesthetic drugs to modify Na+ channel gating currents, a Cys was substituted for the Phe in th

22 nit (only four out of eleven cells exhibited gating currents above the limits of detection, whereas e

23 to simulate the time dependence of the fast gating current allows us to reproduce the observed trend

24 Using voltage-clamp fluorimetry and gating current analysis, we demonstrate that proline sub

25 ubstantiated by measurements of Ca2+ channel gating currents and by analysis of another channel mutat

26 Measurement of gating currents and concentration-dependent availability

27 d in the voltage sensor (S4 region) inducing gating currents and pore opening.

28 G-proteins decreased the amplitude of gating currents and produced depolarizing shifts in the

29 ode-shift was apparent by 24 ms at +60 mV in gating currents, and return of charge closely tracked po

30 Voltage-dependent binding and block of gating current are hallmarks of gating modifier toxins,

31 The properties of the gating currents are in excellent agreement with the prop

32 rack closely with each other, although ASAP1 gating currents are significantly faster than those of C

33 The defunct gating currents are similar in Shaker IR and these two m

34 kinetic steps affected by G-proteins because gating currents arise from the movement of voltage senso

35 ion 666 (TM), affects both ionic current and gating current associated with channel activation, a pre

36 s fast component was found to be part of the gating current associated with the opening and closing o

37 oltage-dependent currents that represent the gating currents associated with CLC-5 activation and had

38 rent and also without blockers, by recording gating current at the reversal potential for ionic curre

39 stal N-terminus (Delta2-135) accelerated off-gating current, but did not influence the relative contr

40 did not shift the voltage-dependence of the gating currents, but reducing and increasing intracellul

41 Here, we show that Q1 gating current can be resolved in the absence of E1, but

42 mentary charge motions that give rise to the gating current cannot be observed directly, but appear a

43 Our results show that normal gating current cannot be stably recorded in the absence

44 Saturating gating currents decreased by two-thirds (K(D) approximat

45 rlying channel gating behavior; by contrast, gating currents directly measure voltage-sensor displace

46 ting charge transfer nor the kinetics of the gating currents during activation.

47 Since the discovery of gating current, electrophysiologists have studied the mo

48 The resulting gating current exhibited a rising phase similar to that

49 ave developed a method for rapidly computing gating currents from a multiparticle ion channel model.

50 uration mutagenesis and detailed analysis of gating currents from gating pore mutations in the Shaker

51 ited by site-3 toxins, we recorded ionic and gating currents from human heart Na channels with mutati

52 By measuring gating currents from the Shaker potassium channel, we de

53 ening transition was used to fit a series of gating currents from the Shaker potassium channel.

54 dent previously in the depolarization-evoked gating currents from voltage-gated Shaker K+ channels ha

55 ltaneously recorded fluorescence signals and gating currents from Xenopus laevis oocytes expressing A

56 The gating currents generated by the mutant channels were on

57 These so-called gating currents have been investigated extensively withi

58 Gating current (I(g)) recorded from a nonconducting muta

59 f thermal noise energy, kT, and to determine gating currents, I express the HH equations in the form

60 Sodium channel ionic current (INa) and gating current (Ig) were compared for rat skeletal (rSkM

61 e of STX, gating kinetics were measured from gating current (Ig).

62 ue to a dramatic slowing of the deactivation gating currents, Ig(D) (with tau up to 80 ms), which dev

63 An early component of the gating current in Shaker K+ channels with a time constan

64 es that allow us to simulate the fluctuating gating current in the activation processes.

65 ge stimulation of one cell in a group evokes gating currents in adjacent OHCs.

66 By measuring gating currents in BK channels coexpressed with chimeras

67 We examined changes in ionic and gating currents in Ca(V)1.2 channels when extracellular

68 is the first demonstration of sodium channel gating currents in single pituitary nerve terminals.

69 and single-channel ionic currents as well as gating currents in tsA201 cells expressing Ca(V)2.2e[37a

70 To test this idea, we recorded NaV1.5 gating currents in Xenopus oocytes using a cut-open volt

71 Previous analysis of eag ionic and gating currents indicated that Mg2+ has a much larger ef

72 regulate the BK voltage sensor, we measured gating currents induced by the pore-forming BK alpha sub

73 o rethink voltage gating models in which the gating current is produced by physical motion of the S4

74 The time constant for the decay of off gating current is very similar to the time constant of d

75 Voltage-shift for gating currents is well documented for voltage-gated cat

76 S4 segment movement, as reflected in gating currents, is almost normal for a 4AP-occupied cha

77 Markov (DSM) model in describing ion channel gating current kinetics.

78 ects are slow compared with motility-related gating current kinetics.

79 Mg2+ has a much larger effect on ionic than gating current kinetics.

80 The gating currents lead to a voltage- and frequency-depende

81 mic-scale movements of charged groups to the gating current measured in an external circuit, however,

82 ue by directly detecting their movement with gating current measurements in 12 F(290) mutants.

83 Gating current measurements revealed that PIP(2) constra

84 teraction between Kv1.3 and NavBeta1 through gating current measurements using the Cut-open Oocyte Vo

85 In this work, we describe macroscopic and gating current measurements, together with molecular mod

86 Here, using mutagenesis and gating current measurements, we show that a 3-fold accel

87 current pulses that generate fluctuations in gating current measurements.

88 Our gating currents measurements showed that NavBeta1 intera

89 The properties of the gating currents (nonlinear charge movements) of human ca

90 sodium conductance and 24.6 +/- 6.8% of the gating current of brain Na(v)1.2a channels, with a speci

91 The properties of ionic and gating currents of alpha 1E were investigated: ionic cur

92 sequence of potassium channels by analyzing gating currents of mutant Shaker IR channels and using t

93 CMMTSET to remove fast inactivation, and the gating currents of R1C-DIV-ICM(MTSET) were recorded befo

94 ates accurately preserved the time course of gating currents on the slow timescale.

95 n the voltage dependence of motility-related gating current or, equivalently, on the voltage dependen

96 ained an alpha-periodicity index of 2.41 for gating current parameters, a new randomization test prod

97 observed in electrophysiology experiments as gating currents preceding ionic conduction.

98 t inactivation with potential alterations of gating current properties.

99 Gating currents recorded from Shaker and I470C were meas

100 By combining giant-patch ionic and gating current recordings in COS-7 cells, and voltage-cl

101 Gating current recordings reveal that mutations at R3 in

102 modulated by Mg2+, have not been detected in gating current recordings.

103 se of activation that is well represented in gating current recordings.

104 ional changes that are poorly represented in gating current recordings.

105 heir kinetics more closely resemble those of gating current records reported for ionic channels.

106 Gating current recovers from inactivation much faster th

107 There is a lag in the onset of gating current recovery at -80 mV, but no lag is discern

108 Our gating current results were reproduced with the addition

109 The model is closely based on the gating current studies of the preceding paper and has be

110 Gating current studies presented here indicate an abnorm

111 experimental data including ionic currents, gating currents, tail currents, steady-state inactivatio

112 ly, produce a distinct fast component in the gating current tails.

113 ctivation is much more voltage dependent for gating current than for ionic current.

114 two, and obtained the current-voltage curve, gating current, the response to a large sine wave (in th

115 HC mechanical activity, the motility-related gating current, to investigate mechano-electrical intera

116 ttenuation in calcium current; the remaining gating current was no different in kinetics or voltage d

117 Interestingly, the "true" mode-shift of gating currents was approximately 40 mV, much greater th

118 By applying lidocaine and measuring the gating currents, we demonstrated that Asn residues in th

119 Gating currents were clearly resolved after ionic curren

120 The gating currents were integrated to measure the intramemb

121 WT and TM gating currents were isolated by replacing Ca2+ with the

122 rrents were measured in 10 mM external Ba2+; gating currents were isolated in 2 mM external Co2+.

123 ne the mechanism of this increase, ionic and gating currents were measured in transiently transfected

124 Gating currents were measured using a variety of inorgan

125 Calcium channel gating currents were recorded after the addition of 5 mM

126 Gating currents were recorded from a double mutant of Sh

127 S4 segment were replaced with histidine, and gating currents were recorded.

128 Gating currents were smaller in cells expressing only th

129 Gating currents were unaffected by verapamil.

130 cells to voltage change is accompanied by a 'gating current', which is manifested as nonlinear capaci

131 changes in the magnitude of motility-related gating currents, which are due predominantly to shifts i

132 rrents, which were modified strongly, and on gating currents, which were not detectably altered.

133 voltage sensor rearrangements with voltage (gating currents) whose movement and associated pore open

134 e was used, membrane depolarization elicited gating current with fast and slow components that differ

135 contains large barriers, which give rise to gating currents with two distinct time scales: the usual

136 n open duration, however, it accelerates off-gating currents without altering on-gating currents.