ライフサイエンスコーパス: gel filtration

1 nalytical ultracentrifugation and analytical gel filtration.

2 graphy, His-Trap affinity chromatography and gel filtration.

3 retained their dimeric aggregation state on gel filtration.

4 ays, surface plasmon resonance analysis, and gel filtration.

5 t species of m.w. 1,400,000 were observed by gel filtration.

6 complex (5NT, Galpha(s), and Gbetagamma) by gel filtration.

7 presence of soluble oligomers observable by gel filtration.

8 milk and various milk fractions obtained by gel filtration.

9 parated into four major fractions (F1-F4) by gel filtration.

10 n during folding in solution was observed by gel filtration.

11 d HeLa cells using column chromatography and gel filtration.

12 ation and other repair proteins, as shown by gel filtration.

13 with both hemin and protoporphyrin IX during gel filtration.

14 nm large disks was detected and isolated by gel filtration.

15 r, in agreement with results from analytical gel filtration.

16 ex with 1 beta-tryptase monomer and 1 Fab by gel filtration.

17 y experiments with chemical cross-linkers or gel filtration.

18 demonstrable by both immunoprecipitation and gel filtration.

19 e chromatography followed by Sephacryl S-300 gel filtration.

20 the MUC2-N trimer eluted as a single peak by gel filtration.

21 ngth, detergent-solubilized EGFR as shown by gel filtration.

22 d the pentameric structure, as determined by gel filtration, 1-anilinonaphthalene-8-sulfonic acid-bin

23 ied by fractionation with ammonium sulphate, gel filtration, affinity and ion exchange chromatography

24 Gel filtration, affinity selection and mass spectrometry

25 High performance liquid chromatography gel filtration analyses of this mini-spectrin provide th

26 Gel filtration analyses show that unphosphorylated DUE-B

27 We show in velocity sedimentation and gel filtration analyses that the mitochondrial DNA helic

28 We used gel filtration analyses to analyze the size of oligomers

29 Confocal microscopy and gel filtration analyses were performed to assess their p

30 Gel filtration analyses, analytical ultracentrifugation

31 Using immunoprecipitation and gel filtration analyses, we found that Holliday junction

32 D-1 ligand by native gel electrophoresis and gel filtration analyses.

33 inity purification, immunoprecipitation, and gel filtration analyses.

34 Gel filtration analysis and blue native gels showed that

35 Gel filtration analysis and cross-linking experiments in

36 Gel filtration analysis of the alphaL260P mini-spectrin

37 Here we use gel filtration analysis of the cytosol of primary and es

38 of NOD2 with another NLR protein, NALP1, and gel filtration analysis revealed the formation of a comp

39 t phosphorylation enhances PKR dimerization, gel filtration analysis reveals a second monomeric phosp

40 concentration-dependent self-association in gel filtration analysis.

41 ex and partially cofractionates with p300 by gel filtration analysis.

42 odeling factor Mi2, were found to coelute by gel-filtration analysis and pull-down assays.

43 Circular dichroism and gel-filtration analysis indicated an extended, unstructu

44 Here we show by gel filtration, analytical ultracentrifugation, and NMR

45 show using a variety of techniques including gel-filtration, analytical ultracentrifugation, electron

46 The gel filtration and 2D gel electrophoresis analysis showe

47 haracterized their oligomerization states by gel filtration and analytical ultracentrifugation experi

48 imer-of-dimers association in agreement with gel filtration and analytical ultracentrifugation studie

49 Gel filtration and analytical ultracentrifugation studie

50 omplex, we undertook an in vitro study using gel filtration and analytical ultracentrifugation.

51 urified in the presence of Nonidet P-40 with gel filtration and anion exchange chromatography.

52 In vivo soluble Cdt1 and PCNA co-elute by gel filtration and associate with each other physically.

53 Gel filtration and biosensor binding experiments reveale

54 Gel filtration and blue native electrophoresis suggest s

55 precipitation or consecutive purification by gel filtration and blue native gel electrophoresis, we d

56 with PME-1 was demonstrated in ripe fruit by gel filtration and by immunoaffinity chromatography.

57 ric complex were characterized by analytical gel filtration and circular dichroism spectroscopy, and

58 cturally folded proteins, as demonstrated by gel filtration and circular dichroism.

59 Gel filtration and co-immunoprecipitation experiments in

60 Analytical gel filtration and covalent cross-linking of purified re

61 Surprisingly, gel filtration and cross-linking analysis showed that a

62 Gel filtration and cross-linking experiments demonstrate

63 Gel filtration and cross-linking of a 71-amino acid frag

64 In RAO-3 cells, Sephacryl-S 300 gel filtration and DEAE-Sepharose ion exchange chromatog

65 LPPS was purified using Sepharose 6B gel filtration and dissolved into two major components,

66 adopt an open conformation, as determined by gel filtration and dynamic light scattering.

67 Analytical gel filtration and electrophoretic mobility shift assays

68 Gel filtration and immunoprecipitation assays show that

69 tear protein-FODE complexes were isolated by gel filtration and ion exchange chromatographies, monito

70 overy using ammonium sulphate precipitation, gel filtration and ion exchange chromatography.

71 n solution and inside cells using analytical gel filtration and luciferase complementation assays and

72 tudied by a combination of western blotting, gel filtration and mass spectrometry.

73 one-target interactions, in combination with gel filtration and molecular dynamics simulations, we he

74 was coeluted with P798-FX cores on both G-75 gel filtration and Ni affinity columns.

75 Importin alpha1 (Impalpha1) was confirmed by gel filtration and NMR studies.

76 S from the mesophile Methanosarcina mazei by gel filtration and nondenaturing mass spectrometry shows

77 Because of concurrent advances in gel filtration and other methods of protein separation,

78 ied as monomer, dimer, and oligomer forms by gel filtration and protein cross-linking.

79 complex was demonstrated by pulldown assays, gel filtration and proximity-dependent biotinylation.

80 Azemiops feae viper venom by combination of gel filtration and reverse-phase HPLC.

81 The epitope off-rate was evaluated by gel filtration and T cell-based assays.

82 Gel filtration and UV spectroscopy reveal that MF1 exist

83 ere characterized by electron microscopy and gel filtration and were found to include annular species

84 crude cell lysates and from fractions after gel filtration and with baculovirally expressed proteins

85 rms through biochemical methods that include gel-filtration and native gel analysis as well as direct

86 Analytical gel-filtration and ultracentrifugation measurements conf

87 Native gel electrophoresis, gel filtration, and analytical ultracentrifugation demon

88 Site-directed mutagenesis, gel filtration, and analytical ultracentrifugation indic

89 ), surface plasmon resonance, cross-linking, gel filtration, and analytical ultracentrifugation studi

90 Using pull-down, gel filtration, and analytical ultracentrifugation studi

91 using NMR, isothermal titration calorimetry, gel filtration, and analytical ultracentrifugation.

92 purified by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography.

93 ance spectroscopy, dynamic light scattering, gel filtration, and autophosphorylation kinetics.

94 Kinetic analysis, analytical gel filtration, and chemical cross-linking demonstrated

95 Using NMR spectroscopy, gel filtration, and chemical cross-linking, we obtained

96 Physicochemical analyses using native gels, gel filtration, and differential scanning fluorimetry re

97 acterized by analytical ultracentrifugation, gel filtration, and electron microscopy.

98 Freshly dissolved EMD was fractionated by gel filtration, and forty-five 7-ml fractions were colle

99 ic by confocal microscopy, western blotting, gel filtration, and immunoprecipitation studies in macro

100 After purification by affinity, gel filtration, and ion exchange chromatography, NR3A S1

101 for iNOS, using rapid stopped-flow kinetic, gel filtration, and spectrophotometric analysis.

102 e native polyacrylamide gel electrophoresis, gel filtration, and transmission electron microscopy of

103 ed using ammonium sulphate precipitation and gel-filtration, and subjected to multiple enzymatic dige

104 tly bound protein factors can be purified by gel filtration as a functional entity called the transcr

105 n assays confirmed their interactions, while gel filtration assay indicated that C53/LZAP and RCAD ma

106 Indeed, gel filtration assays demonstrated that although nucleot

107 s insertion into the PDZ binding pocket, and gel filtration assays showed that this phosphomimic muta

108 Mutagenesis combined with gel filtration assays suggested that the side chain hydr

109 by sucrose-loaded vesicle sedimentation and gel filtration assays, and the effects of pH and lipid c

110 Using co-immunoprecipitation and gel filtration assays, we show that CPEB specifically in

111 When examined by gel filtration at 0.5 M NaCl in the absence of DNA, Rep6

112 ry at approximately 50 kDa and by denaturing gel filtration at approximately 50-61 kDa, while its nat

113 rm rapidly and are stable in solution during gel filtration at low pH.

114 g assay and scintillation proximity assay or gel filtration binding assays using (3)H-labeled PPARalp

115 aracterization of cVIMP-Cys using analytical gel filtration, CD and NMR spectroscopy in conjunction w

116 ing only of the repeat-containing regions by gel filtration, CD spectroscopy, and negative-staining e

117 Using gel filtration, chemical cross-linking, analytical ultra

118 In gel filtration chromatography analyses of BC-3 cell lysa

119 inary approach, including live cell imaging, gel filtration chromatography analysis, split ubiquitin

120 TS co-migrated exclusively with USP9X during gel filtration chromatography analysis.

121 Gel filtration chromatography and analytical ultracentri

122 Analyses by gel filtration chromatography and analytical ultracentri

123 Using a combination of gel filtration chromatography and analytical ultracentri

124 Tear proteins were separated by gel filtration chromatography and analyzed for bound lip

125 Gel filtration chromatography and co-immunoprecipitation

126 trary to previous findings, a combination of gel filtration chromatography and co-purification studie

127 mbination of ion-exchange chromatography and gel filtration chromatography and demonstrated for the f

128 Supplemented with gel filtration chromatography and fluorescence-adapted s

129 Both enzymes were found to be homodimeric by gel filtration chromatography and homotetrameric by dyna

130 Second, gel filtration chromatography and immunoprecipitation in

131 Using gel filtration chromatography and isothermal titration c

132 Both gel filtration chromatography and mass analysis from two

133 dimer to a monomeric protein on the basis of gel filtration chromatography and multiple-angle laser l

134 on procedures including membrane filtration, gel filtration chromatography and reversed-phase high-pe

135 Gel filtration chromatography and SDS-PAGE revealed that

136 Analytical gel filtration chromatography and sedimentation velocity

137 tration membranes was further purified using gel filtration chromatography and two steps of reverse-p

138 nuclein was quantified by the combination of Gel filtration chromatography and Western blot, respecti

139 Purification through gel filtration chromatography confirmed the formation of

140 Gel filtration chromatography confirms the differentiati

141 ulldown assays of a Orai1-CMBD(W76E) mutant, gel filtration chromatography data, and NOE signals indi

142 Analytical gel filtration chromatography demonstrates that Slit D2

143 Gel filtration chromatography demonstrates that upon bin

144 Fractionation of plasma by gel filtration chromatography followed by bioassays indi

145 The resolving exudate was subjected to gel filtration chromatography followed by proteomics, id

146 se peptides were purified after affinity and gel filtration chromatography from a chickpea protein hy

147 0 Da, relative to globular standards, during gel filtration chromatography in the absence of polyanio

148 Fractionation of serum by gel filtration chromatography led to the identification

149 Gel filtration chromatography of conditioned medium from

150 In the present studies, gel filtration chromatography of liver cytosol from LFAB

151 During gel filtration chromatography of solubilized SR membrane

152 Gel filtration chromatography of the full-length fusion

153 Gel filtration chromatography of the purified protein re

154 olysis, circular dichroism spectroscopy, and gel filtration chromatography revealed that the N-termin

155 In addition, gel filtration chromatography separated multimeric and m

156 variant, we used chemical cross-linking and gel filtration chromatography to show that each exists a

157 C5 was demonstrated by Western blotting and gel filtration chromatography using 125I-labeled protein

158 s resolved from the excision activity during gel filtration chromatography using Sephacryl S-300.

159 hromatography using DEAE-Toyopearl 650 M and gel filtration chromatography using Sephadex G-100.

160 n-dodecyl beta-D-maltoside (DDM) examined by gel filtration chromatography was generally modest, and

161 rate the potential for real-time monitoring, gel filtration chromatography was used to separate diffe

162 crylamide gel electrophoresis and analytical gel filtration chromatography were used to detect the di

163 5 kD fraction (Ara h 2/6; 20 kD fraction) on gel filtration chromatography, account for the majority

164 , we used a combination of NMR spectroscopy, gel filtration chromatography, and analytical ultracentr

165 Using native gel electrophoresis, gel filtration chromatography, and surface plasmon reson

166 nd the methods of gel mobility shift assays, gel filtration chromatography, and UV-cross-linking, we

167 rm agglutinin chromatography, Sephacryl S300 gel filtration chromatography, anti-FLAG immunoaffinity

168 The native molecular mass, as determined by gel filtration chromatography, appeared to be approximat

169 As determined by gel filtration chromatography, cGMP binding caused elong

170 Using a combination of gel filtration chromatography, differential scanning cal

171 med structural studies of this protein using gel filtration chromatography, intrinsic tryptophan fluo

172 ion bodies, but when subjected to analytical gel filtration chromatography, it elutes in the void vol

173 Here, using gel filtration chromatography, sedimentation, fluorescen

174 We have used gel filtration chromatography, site-directed mutagenesis

175 ecombinant protein expression and analytical gel filtration chromatography, surface plasmon resonance

176 Using gel filtration chromatography, we assessed the effects o

177 s, PCNA-coupled affinity beads pull-down and gel filtration chromatography, we show that the same reg

178 was stable during affinity purification and gel filtration chromatography.

179 measurements below the polymer's LCST using gel filtration chromatography.

180 metal affinity, hydrophobic interaction, and gel filtration chromatography.

181 ueous hydrogen fluoride (HF) and purified by gel filtration chromatography.

182 roteins were purified by Ni-NTA affinity and gel filtration chromatography.

183 apientum (MSP) were isolated and purified by gel filtration chromatography.

184 s p50 were found to co-fractionate following gel filtration chromatography.

185 navalin A-Sepharose affinity and Superose 12 gel filtration chromatography.

186 exchange chromatography, ultrafiltration and gel filtration chromatography.

187 fied using ultrafiltration, ion exchange and gel filtration chromatography.

188 The hydrolysate was further purified by gel filtration chromatography.

189 pitation with ammonium sulphate, followed by gel filtration chromatography.

190 ein still elutes in the void upon analytical gel filtration chromatography.

191 led under aprotic conditions and purified by gel-filtration chromatography (GFC) using high-specific-

192 Gel-filtration chromatography and analytical ultracentri

193 A combination of gel-filtration chromatography and circular dichroism spe

194 A combination of gel-filtration chromatography and protein overlay assays

195 Analytical gel-filtration chromatography confirms that the OMP.OPRT

196 of PDE3A2 by PKC led to shifts in elution on gel-filtration chromatography consistent with increased

197 Gel-filtration chromatography demonstrated an interactio

198 Gel-filtration chromatography demonstrated that ASRT for

199 However, examination of P-TEFb complexes by gel-filtration chromatography showed that TCR signaling

200 ass spectrometry, co-immunoprecipitation and gel-filtration chromatography to define higher-order pro

201 Plasma was fractionated using gel-filtration chromatography, and lipid-associated prot

202 t molecular mass of 110 kDa as determined by gel-filtration chromatography, indicating that the prote

203 We characterized NABBs by gel-filtration chromatography, native polyacrylamide gra

204 Bioactive peptide separated by gel-filtration chromatography, showed the higher antioxi

205 ated with high density lipoproteins (HDL) by gel-filtration chromatography, we found no evidence that

206 Using sequential size-exclusion and gel-filtration chromatography, we identified a condition

207 ricted to oligomer-containing fractions from gel-filtration chromatography.

208 dentical subunits) that the complex survives gel-filtration chromatography.

209 orresponding to approximately 170-180 kDa by gel-filtration chromatography.

210 rmed large aggregates that did not enter the gel filtration column but were made visible after densit

211 Our gel filtration column chromatography and cross-linking s

212 LpxL migration on a gel filtration column is consistent with a molecular mas

213 gent-solubilized protein migration through a gel filtration column toward smaller molecular masses wi

214 The factor eluted from the gel filtration column with an apparent molecular mass of

215 lowed by mass spectrometry, coelution from a gel filtration column, cosedimentation on a glycerol gra

216 MGAT2 also co-eluted with DGAT1 on a gel filtration column, suggesting that the two enzymes f

217 using a cation exchange column followed by a gel filtration column.

218 h-performance frontal analysis using a short gel-filtration column and phosphate buffered saline solu

219 ant larger liposomes are passed over another gel-filtration column to exchange an extraliposomal high

220 All the collected effluent from the gel-filtration column was then transferred to the second

221 nd chromatography through DEAE-Sepharose and gel filtration columns.

222 Furthermore, ABCG5/G8 eluted as a dimer on gel filtration columns.

223 Gel filtration confirmed that purified E. aediculatus te

224 Light scattering coupled to gel filtration confirms the monomeric state of solubiliz

225 ative state of alpha-synuclein was probed by gel filtration coupled with native gradient gel separati

226 with high-performance liquid chromatography gel filtration data and demonstrate that single-molecule

227 versible thermal denaturation and analytical gel filtration data suggest that the central CCM-1 is cr

228 Gel filtration demonstrated that the N1347S mutation dis

229 etory pathway we analyzed purified MUC2-N by gel filtration, density gradient centrifugation, and tra

230 assays) and multimerization (as revealed by gel filtration, dynamic light scattering, and analytical

231 as judged by analytical ultracentrifugation, gel filtration, dynamic light scattering, and CD.

232 DS resistance assay, antibody binding assay, gel filtration, dynamic light scattering, small angle x-

233 Gel-filtration, dynamic light scattering, circular dichr

234 The gel filtration elution profile and the small angle x-ray

235 Gel filtration experiments indicate that both Rtg2p and

236 Gel filtration experiments indicate that MtATP-phosphori

237 t lacks an active site cysteine residue, and gel filtration experiments show that it resides in a hig

238 kably, gel shift, chemical cross-linking and gel filtration experiments showed that S326C both exists

239 Gel filtration experiments using wild-type and mutated X

240 mers in vitro, as shown in cross-linking and gel filtration experiments.

241 lexes, as judged by bacterial monohybrid and gel filtration experiments.

242 ically from pooled stimulated human tears by gel filtration fast performance liquid chromatography.

243 ned the quinone content in lipid extracts of gel filtration fractions by liquid chromatography-tandem

244 olines between m/z 490 and 540 in any of the gel-filtration fractions.

245 binds tightly to human platelets even after gel filtration, has a prolonged half-life in mice transg

246 ically active EGFR dimers can be isolated by gel filtration in dodecyl maltoside.

247 the two form a complex, further suggested by gel filtration, in which the HMGBs with core histones el

248 However, as gel filtration indicated sufficient heterodimer was pres

249 Gel filtration indicated that the mutations did not affe

250 Analytical gel filtration indicated that the protein exists in solu

251 Analysis of the mutants by gel filtration indicates that at least five residues mus

252 Gel filtration indicates that the protein is not a dimer

253 me has a measured molecular mass of 135 kDa (gel filtration), indicating it is a trimer.

254 we report on chromatographic fractionation (gel filtration, ion exchange, and hydroxyapatite) of ext

255 We used gel retardation, gel filtration, isothermal titration calorimetry and NMR

256 Pulldown assays, gel filtration, isothermal titration calorimetry, and ra

257 methods, including native gel and analytical gel filtration, isothermal titration calorimetry, NMR sp

258 characteristics of collagen, we developed a gel filtration-like procedure that uses columns containi

259 sD readily formed a complex that elutes from gel filtration medium as a single included peak.

260 Here, we developed a simple gel filtration method to enrich semiconducting (12,1) an

261 Using novel flow cytometry and gel filtration methods, we quantified SS1P uptake in ind

262 Gel filtration, native PAGE, and protein cross-linking w

263 Gel filtration of HPAEC-CM revealed a peak of eosinophil

264 We performed gel filtration of liver supernatants and identified two

265 Analytical gel filtration of purified protein and cryo-electron mic

266 hown by isothermal titration calorimetry and gel filtration of recombinant subunits.

267 Gel filtration of the affinity-purified material further

268 Interestingly, gel filtration of this substrate-binding mutant also det

269 hange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75.

270 er various conditions has been studied using gel filtration or analytical ultracentrifugation experim

271 ntly removed from protein-lipid complexes by gel filtration or dialysis into high potassium (high [K+

272 gomers were characterized concerning size by gel filtration, polyacrylamide gel electrophoresis, and

273 tural information of eluted materials in the gel filtration provides important clues about their beha

274 odimer of 28 kDa subunits on Superdex HR 200 gel filtration resin.

275 column chromatography on anion-exchange and gel filtration resins.

276 mers and dimers in solution, and equilibrium gel filtration revealed a 2:1 receptor/ligand binding st

277 Fractionation of rLcrV preparations via gel filtration revealed that only a minor component cons

278 Using analytical gel filtration, sedimentation-velocity ultracentrifugati

279 C), 80% ammonium sulfate precipitation, and gel filtration (Sephadex G25).

280 Chemical cross-linking and gel-filtration show that a 1:1 linker histone/HMGB1 comp

281 Furthermore, sedimentation velocity and gel filtration showed that NEMO ZF, like other NEMO rela

282 Gel filtration showed that the open channel conformers t

283 haracterized the allosteric inhibition using gel filtration, steady-state and pre-steady-state kineti

284 ution, typically through a final dialysis or gel filtration step.

285 hift assays and footprinting, cross-linking, gel filtration stoichiometry, and DNA bending assays.

286 Circular dichroism spectroscopy and gel filtration studies confirm that this region has a he

287 Gel filtration studies indicated that 7B2 exists as an e

288 In addition, gel-filtration studies showed that formation of chaperon

289 and endogenous proteins and co-elution with gel filtration suggest that an endogenous Ajuba.Gfi1.HDA

290 Gel filtration suggested that DeltaKal7, a natural isofo

291 han the inactive holoenzyme when analyzed by gel filtration, suggesting an expanded conformation.

292 ar weight ATPase-DNA complex was isolated by gel filtration, suggesting oligomerization of ATPase fol

293 Gel filtration suggests that FDM1 may exist as a homodim

294 , different forms of Rho were isolated using gel filtration techniques in mild detergents, including

295 We show by sedimentation and gel filtration that Nck1 and CE are together in a larger

296 t Y118D alphaA-crystallin were studied using gel filtration, two-dimensional (2D) gel electrophoresis

297 ajority of which are subsequently removed by gel filtration using beads with an exclusion limit of 40

298 In addition, using gel filtration, we find that the conformational opening

299 :1, 1:2, and 1:3 complexes were confirmed by gel filtration with in-line light scattering.

300 A system combining pressurized capillary gel filtration with online laser light scattering and mi