1 rried out on the protein recovered from each gel slice.

2 cised, and quantitatively extracted from the gel slices.

3 f phosphotyrosine were found in only 2 of 60 gel slices.

4 is, and tryptic peptides were extracted from gel slices and analyzed by liquid chromatography-tandem

5                   Protein was extracted from gel slices and subjected to tryptic digestion and analys

6 t or heavy isotope-labeled reagents, and the gel slices are then combined and digested with proteases

7  peptides in a series of experiments using a gel slice containing only 200 ng (3 pmol) of human doubl

8                                        These gel slices corresponded to M(r)'s of 42 and 40 kilodalto

9 ed for Western blotting of cell lysates, and gel slices corresponding to reactive bands were subjecte

10 d electroelution of protein factors from the gel slices corresponding to the ribonucleoprotein comple

11 ution is usually achieved by heating excised gel slices in a small volume of either water or TE.

12 ethod involves homogenization of the excised gel slice (in Tris-EDTA buffer), containing the DNA frag

13 ations where the amount of DNA in an excised gel slice is limited or when contaminating differential

14 imple method for extracting DNA from agarose gel slices is described.

15 plexes and RNase ONE footprinting in situ in gel slices or by RNase H cleavage of complexes in soluti

16 proteins in solution, as well as proteins in gel slices, prior to proteolytic digestion and mass spec

17 el electrophoresis and subsequent elution of gel slices revealed the presence of three active moietie

18                                          For gel slices, the procedure combines washing, destaining,

19 tive DNA coupled with PCR amplification from gel slices, we show that specific binding sites can be i

20                                 Thirty-three gel slices were excised from each of three gel lanes (n

21 ution rather than the more common melting of gel slices with subsequent beta-agarase treatment.

22           Phosphotyrosine was found in every gel slice, with two major peaks of this phosphoamino aci