ライフサイエンスコーパス: gelatinase B

1 MMP-1 (interstitial collagenase) and MMP-9 (gelatinase B).

2 or interactions, still inhibits synthesis of gelatinase B.

3 ype MMP-1, collagenase 1, stromelysin 1, and gelatinase B.

4 inding protein and the active site region of gelatinase B.

5 tions of term placenta, is co-localized with gelatinase B.

6 fter intratracheal bleomycin irrespective of gelatinase B.

7 ctin, which is not a substrate for mammalian gelatinase B.

8 elatinase is likely the avian counterpart of gelatinase B.

9 to be biochemically divergent from mammalian gelatinase B.

10 cytomas secrete the 92-kDa proenzyme form of gelatinase B.

11 ctivities against the MMPs collagenase-1 and gelatinase-B.

12 expression of matrix metalloproteinase MMP-9/gelatinase-B.

13 llagen domain that is found in all mammalian gelatinase Bs.

14 concentration (P < 0.001), and a greater pro-gelatinase B (92 kDa) activity (P < 0.001) in their tear

15 ncoding the complete sequence of mastocytoma gelatinase B, a 2.3-kilobase clone encoding progelatinas

16 Gelatinase B, a matrix metalloproteinase (MMP) of high s

17 Gelatinase B, a matrix metalloproteinase that has proteo

18 alpha concentration was correlated with tear gelatinase B activity (p=0.58, P < 0.002).

19 ation (p=0.67, P < 0.001) and increased tear gelatinase B activity (p=0.84, P < 0.001).

20 Elevated gelatinase B activity in ocular rosacea may be involved

21 era from tumor-bearing animals had increased gelatinase B activity that was inhibited by CT1746 level

22 both Northern blot analysis and zymography, gelatinase B activity was detected by zymography in two

23 urvival can be improved by inhibiting MMP-9 (gelatinase B) activity.

24 The 92-kDa type IV collagenase (92-kDa gelatinase B also referred to as MMP-9), which plays a c

25 In BR cells, gelatinase B and alpha-chymase expression are regulated,

26 eless, in human corneas with repair defects, gelatinase B and collagenase are synthesized by cells in

27 Since elevated levels of both gelatinase B and collagenase-3 have been observed in art

28 ty of chimeric enzymes containing domains of gelatinase B and fibroblast collagenase.

29 response despite the continued expression of gelatinase B and metalloelastase.

30 We report longitudinal serum levels of gelatinase B and of the tissue inhibitors of matrix meta

31 sion of the inducible nitric oxide synthase, gelatinase B and scavenger receptor A genes in response

32 n with accompanying expression of neutrophil gelatinase B and upregulation of gp91phox.

33 yme appears structurally distinct from known gelatinase Bs and the activated enzyme can cleave fibron

34 Increased levels of expression of MMP-9 (gelatinase B) and MMP-2 (gelatinase A) have been observe

35 In contrast, MMP-1 (collagenase-1), MMP-9 (gelatinase B), and MMP-12 (macrophage elastase) cleaved

36 omelysin 1) in astrocytes, and MMP-1, MMP-9 (gelatinase B), and TIMP-1 in fibroblasts.

37 n collagenase 3 over the MMPs stromelysin-1, gelatinase B, and collagenase 1, respectively.

38 At 4 days after injury, TIMP-1, gelatinase B, and TNF-alpha mRNAs were localized to infi

39 pecifically, MMP-2 (gelatinase A) and MMP-9 (gelatinase B) are strongly associated with malignant pro

40 For similar levels of serum gelatinase B, associated TIMP-1 levels were significantl

41 MMP-13 may play an important role in the gelatinase B-associated proteolytic cascade that allows

42 lpha-chymase cleaves the catalytic domain of gelatinase B at the Phe88-Gln89 and Phe91-Glu92 bonds.

43 ent incongruity, we examined the activity of gelatinase B bound to either gelatin- or type IV collage

44 odestly increased trabecular stromelysin and gelatinase B but not collagenase, gelatinase A, or tissu

45 Expression of gelatinase B by the high grade B-cell Burkitt's lymphoma

46 In this study, we show that production of gelatinase B by these cells is similarly affected by cel

47 By introducing chicken gelatinase B (chMMP-9) as a specific marker for heteroph

48 vation of the matrix metalloproteinase MMP-9/gelatinase B coincides with the angiogenic switch in pre

49 Gelatinase B, collagenase-3, and metalloelastase express

50 f plasminogen, the gelatinase A/TIMP-2 axis, gelatinase B, collagenase-3, collagenase-2, or stromelys

51 as of embryonic day 13-15 mandibles, whereas gelatinase B, collagenase-3, TIMP-1 and TIMP-2 mRNA were

52 we instilled bleomycin intratracheally into gelatinase B-deficient mice and gelatinase B+/+ litterma

53 Unlike stromelysin-1-deficient mice, gelatinase B-deficient mice exhibited a CHS response com

54 Gelatinase B-deficient mice failed to show IL-10 product

55 However, gelatinase B-deficient mice had a normal rate of resolut

56 However, gelatinase B-deficient mice reconstituted with neutrophi

57 However, the lungs of gelatinase B-deficient mice showed minimal alveolar bron

58 Gelatinase B-deficient mice were bred with Col4a3-/- mic

59 Gelatinase B-deficient mice were resistant to the bliste

60 cant neutrophil recruitment into the skin in gelatinase B-deficient mice, but blistering did not occu

61 Active gelatinase B did not contribute to the activation rate o

62 romal fibroblasts, production (synthesis) of gelatinase B does not appear to be controlled by secrete

63 Here, we show that MMP-9 (gelatinase B; EC.3.4.24.35) contributes to the pathophys

64 d cord and matrix metalloproteinase-9 (MMP-9/gelatinase B), expressed by infiltrating monocytes.

65 icating that these processes are mediated by gelatinase B-expressing cells of bone marrow origin, des

66 Increased gelatinase A and gelatinase B expression (demonstrated by in situ hybridi

67 nderwent apoptosis during the main period of gelatinase B expression and ectoplacental growth and exp

68 nal requirements for the induction of 92-kDa gelatinase B expression by an activated ras oncogene.

69 ul model for investigating the regulation of gelatinase B expression in vivo and for identifying and

70 his correlates with inhibition of endogenous gelatinase B expression induced by FGF-2.

71 Because gelatinase B expression is linked to invasive potential,

72 attenuates kit ligand-mediated induction of gelatinase B expression, suggesting that an excess of TG

73 ed matrix metalloproteinase-9 (also known as gelatinase-B) expression and coincided with inhibition o

74 rch the promoter region of the gene encoding gelatinase B for naturally occurring genetic variation.

75 he transcriptional promoter of the MMP gene, gelatinase B (gelB), in transgenic mice, demonstrating t

76 Gelatinase B (gelB; also known as MMP-9) is expressed in

77 l promoter for the matrix metalloproteinase, gelatinase B (gelB; MMP-9) in cell culture transfection

78 ied the role of the matrix metalloproteinase gelatinase B (gelB; MMP-9) in epithelial regeneration us

79 e in levels of the matrix metalloproteinase, gelatinase B (GelB; MMP-9).

80 algranulin A, cathepsin B and D, E-cadherin, gelatinase B, gelsolin, interstitial collagenase, lamini

81 ied mechanisms controlling activation of the gelatinase B gene (matrix metalloproteinase-9) by fibrob

82 sequences between -522 and +19 of the rabbit gelatinase B gene (MMP-9) (as characterized in the trans

83 ygous mice with a null mutation in the MMP-9/gelatinase B gene exhibit an abnormal pattern of skeleta

84 ence variation in the promoter region of the gelatinase B gene influences its expression, predisposin

85 this functional genetic variation influences gelatinase B gene promoter activity in an allele-specifi

86 nd characterizing new drugs that can control gelatinase B gene transcription.

87 otides of 5' flanking sequence of the 92-kDa gelatinase B gene.

88 To assess the role of gelatinase B in bleomycin-induced fibrosing alveolitis,

89 for controlling pathologic overproduction of gelatinase B in corneal ulcers.

90 pe IV collagen from degradation by exogenous gelatinase B in cryostat sections of nerve in vitro.

91 signaling pathway and inhibit expression of gelatinase B in the angiogenic process.

92 In this study we investigated the role of gelatinase B in the immunopathogenesis of experimental B

93 These results implicate neutrophil-derived gelatinase B in the pathogenesis of experimental BP and

94 ate that a Plg cascade synergizes with MMP-9/gelatinase B in vivo during dermal-epidermal separation

95 nd activation of metalloproteinase-9 (MMP-9, gelatinase B) in a well-established steatotic rat liver

96 and activity of MMP-2 (gelatinase A) and -9 (gelatinase B) in cardiac fibroblasts.

97 gated the role of metalloproteinase-9 (MMP-9/gelatinase B) in liver ischemia/reperfusion (I/R) injury

98 or gelatinases (MMP-2/gelatinase-A and MMP-9/gelatinase-B) in apolipoprotein E-deficient (apoE-/-) mi

99 egion of fibroblast collagenase with that of gelatinase B increased the catalytic efficiency of the e

100 decreased tissue expression of TNF-alpha and gelatinase B, indicating both systemic and local actions

101 have studied the expression of gelatinase A, gelatinase B, interstitial collagenase, tissue inhibitor

102 Gelatinase B is a member of the matrix metalloproteinase

103 Unlike gelatinase A, gelatinase B is expressed exclusively by migrating basal

104 Gelatinase B is present in BP blister fluid and can clea

105 However, gelatinase B is required for alveolar bronchiolization,

106 The matrix metalloproteinase gelatinase B is synthesized by cells at the leading edge

107 The matrix metalloproteinase MMP-9/gelatinase B is upregulated in angiogenic dysplasias and

108 Although MMP-9/gelatinase-B is an important component of these TGF-beta

109 One of these gelatinases, gelatinase B, is synthesized by the resident corneal cel

110 iple sclerosis patients with high mean serum gelatinase B levels had significantly more T1-weighted g

111 In the patients with multiple sclerosis, gelatinase B levels were significantly higher during cli

112 exists in chickens, and although it is MMP-9/gelatinase B-like in its overall domain structure and ex

113 cheally into gelatinase B-deficient mice and gelatinase B+/+ littermates.

114 Expression of gelatinase B (matrix metalloprotease 9) in human placent

115 e elastase (matrix metalloproteinase-12) and gelatinase B (matrix metalloproteinase-9).

116 of the extracellular matrix-degrading enzyme gelatinase B/matrix metalloproteinase-9 (Mmp-9) on islet

117 ferentiation of chondroclasts, which express gelatinase B/matrix metalloproteinase-9, and resorption

118 uman, mouse, and rabbit 92-kD progelatinase (gelatinase B; matrix metalloproteinase-9).

119 alpha-chymase in tissue remodeling involving gelatinase B-mediated degradation of matrix proteins.

120 lized cells 7 and 14 days after bleomycin in gelatinase B+/+ mice, and whole lung gelatinase B mRNA w

121 onchiolization was prominent in the lungs of gelatinase B+/+ mice.

122 trix metalloproteinase (MMP) 26-mediated pro-gelatinase B (MMP-9) activation promoted invasion of hum

123 Gelatinase B (MMP-9) activity is greater in patients wit

124 ein expression corresponds to an increase in gelatinase B (MMP-9) activity, which is greatly upregula

125 metalloproteinases gelatinase A (MMP-2) and gelatinase B (MMP-9) after partial hepatectomy.

126 esponding to the zymogen and active forms of gelatinase B (MMP-9) and gelatinase A (MMP-2) and to the

127 approximately 125-kDa as being a complex of gelatinase B (MMP-9) and neutrophil gelatinase-associate

128 ) were used to measure gelatinase A (MMP-2), gelatinase B (MMP-9) and tissue inhibitors of metallopro

129 Gelatinase A (MMP-2) and gelatinase B (MMP-9) are able to digest the endothelial

130 SDF-1 induces the expression and release of gelatinase B (MMP-9) by purified mature polyploid human

131 ith deficiencies of stromelysin-1 (MMP-3) or gelatinase B (MMP-9) in a dinitrofluorobenzene (DNFB)-in

132 sion of interstitial collagenase (MMP-1) and gelatinase B (MMP-9) that was reversed by PGE2 or Bt2cAM

133 al collagenase (MMP-1), stromelysin (MMP-3), gelatinase B (MMP-9), and activated gelatinase A (MMP-2)

134 he Mr 72,000 gelatinase A (MMP-2), Mr 92,000 gelatinase B (MMP-9), and TIMP-2 to evaluate their signi

135 n of matrix metalloproteinases, particularly gelatinase B (MMP-9), has been described in the lungs in

136 of CD40 on ECs induced de novo expression of gelatinase B (MMP-9), increased interstitial collagenase

137 The effect of Brij-35 on human gelatinase B (MMP-9), matrilysin (MMP-7), and membrane-t

138 We showed that the MMPs gelatinase B (MMP-9), stromelysin-1 (MMP-3), and the tis

139 latinase A (MMP-2), stromelysin (MMP-3), and gelatinase B (MMP-9).

140 tinase A (MMP-2), stromelysin-1 (MMP-3), and gelatinase B (MMP-9).

141 c- teristically produce the 92-kDa mammalian gelatinase B (MMP-9).

142 g mice containing targeted disruption of the gelatinase B (MMP-9, 92 kD gelatinase) gene.

143 have identified the key protein substrate of gelatinase B/MMP-9 (GB) that is cleaved in vivo during d

144 kage within 30-60 min, likely independent of gelatinase B/MMP-9 activities.

145 pairment of chondroclastic resorption, lower gelatinase B/MMP-9 activity, decline in insulin-like gro

146 sive potential, we studied the expression of gelatinase B mRNA and protein in vivo, in implanting tro

147 produced a approximately 15-fold increase in gelatinase B mRNA expression, dexamethasone down-regulat

148 ic cytokine TGF-beta virtually abolishes the gelatinase B mRNA signal and also attenuates kit ligand-

149 ycin in gelatinase B+/+ mice, and whole lung gelatinase B mRNA was increased at the same times.

150 paralleled by an upregulation of TIMP-1 and gelatinase B mRNAs.

151 rescues vascularization and ossification in gelatinase B-null growth plates, indicating that these p

152 Growth plates from gelatinase B-null mice in culture show a delayed release

153 ix metalloproteinase 9 (MMP-9, also known as gelatinase B or 92-kd Type IV collagenase) is overexpres

154 bution of matrix metalloproteinase-9 (MMP-9, gelatinase B or the 92-kd type IV gelatinase/collagenase

155 MMP-9 (gelatinase B or the 92-kDa gelatinase/type IV collagenas

156 ession of matrix metalloproteinase-9 (MMP-9, gelatinase B), persistent extracellular matrix, and fail

157 ired for initiation of the response, whereas gelatinase B plays a critical role in its resolution.

158 s to release pro-matrix metalloproteinase-9 (gelatinase B; pro-MMP-9) and active matrix metalloprotei

159 In contrast, gelatinase B processed pIL-1 beta within minutes, result

160 cur in parallel: IL-1alpha does not regulate gelatinase B production (synthesis), nor was there evide

161 re was to investigate mechanisms controlling gelatinase B production by corneal epithelial cells.

162 Instead, our data suggest that gelatinase B production is downregulated directly by hig

163 cytokine that has been reported to stimulate gelatinase B production.

164 xpression vector was insufficient for 92-kDa gelatinase B promoter activation.

165 ng pathway mediating the induction of 92-kDa gelatinase B promoter activity by ras was examined.

166 members abrogated the stimulation of 92-kDa gelatinase B promoter activity by ras.

167 2, and Sp1) that control the activity of the gelatinase B promoter are selectively induced in the epi

168 K1) did not prevent activation of the 92-kDa gelatinase B promoter by ras and a constitutively activa

169 Thus, the stimulation of the 92-kDa gelatinase B promoter by ras requires multiple elements

170 al and induced activity of the canine MMP-9, gelatinase B promoter in these cell types.

171 driven by 5' deleted fragments of the 92-kDa gelatinase B promoter indicated that a region spanning -

172 the ras-dependent stimulation of the 92-kDa gelatinase B promoter-driven CAT reporter.

173 ild type but not the PEA3/ets-deleted 92-kDa gelatinase B promoter.

174 g a transgenic mouse (line 3445) harboring a gelatinase B promoter/lacZ fusion gene, we demonstrate F

175 As shown for proMMP-9 (type IV gelatinase-b) proteolytic activity can be quantitated in

176 articular, MMP-2 and MMP-9 (gelatinase A and gelatinase B, respectively) have been identified as the

177 hese results indicate that stromelysin-1 and gelatinase B serve important functions in CHS.

178 xpression in whole-lung homogenates, whereas gelatinase B, stromelysin-1, and interstitial collagenas

179 Gelatinase A, gelatinase B, stromelysin-1, urokinase, TIMP-1 and TIMP-

180 r their ability to inhibit collagenase-1 and gelatinase-B substrate hydrolysis.

181 to inhibit collagenase-1, stromelysin-1, and gelatinase-B substrate hydrolysis.

182 human matrix metalloproteinase-9 (MMP-9, or gelatinase B) suggested the identity of this activity, w

183 n induces matrix metalloproteinase-9 (MMP-9; gelatinase B) synthesis by monocytes, a process that is

184 ), Asp(410), and Pro(415), in this region of gelatinase B that are important for its efficient cataly

185 Average serum gelatinase B, TIMP-1 and TIMP-2 levels were significantl

186 e addition of the fibronectin-like domain of gelatinase B to fibroblast collagenase is sufficient to

187 We further show that induction of gelatinase B transcriptional promoter activity in respon

188 teinase (MMP) family, matrilysin (MMP-7) and gelatinase B/type IV collagenase (MMP-9), hydrolyze huma

189 Gelatinase B was identified immunohistochemically in ter

190 Gelatinase B was immunolocalized to the basement membran

191 The 84-kDa active form of gelatinase B was observed in 46% of the rosacea tear sam

192 metalloelastase expression were more focal; gelatinase B was primarily localized to endothelial cell

193 n 10-fold selectivity for collagenase-1 over gelatinase-B was desired.

194 bFGF) and matrix metalloproteinase 9 (MMP-9, gelatinase B) was found to be associated with the format

195 ted human matrix metalloproteinase-9 (MMP-9, gelatinase B) was produced from the proenzyme by limited

196 influx, actin polymerization, and release of gelatinase B were comparable to those of wild-type PMNs.

197 l mice, the MMPs MT1-MMP, stromelysin 3, and gelatinase B were expressed at low levels, whereas high

198 lloproteinase (MMP)-2 (gelatinase A) and -9 (gelatinase B) were synthesized and secreted into the med

199 MMP-2 (gelatinase A) and MMP-9 (gelatinase B) were the only two MMPs expressed.

200 , the protein is only 59% identical to human gelatinase B, whereas all previously cloned chicken MMP

201 st, non-chymase-producing C1 cells secrete a gelatinase B (which remains in its proform) only in resp

202 lloproteinases, MMP-2/gelatinase A and MMP-9/gelatinase B, which are known to have critical roles in

203 We found that addition of the active site of gelatinase B, which corresponds to 12% of the total prot

204 ed tissues may alter mast cell expression of gelatinase B, which is implicated in extracellular matri

205 conducted for stromelysin, gelatinase A, and gelatinase B with the various inhibitors.

206 itors (IC50's < 100 nM) of collagenase-1 and gelatinase-B, with the most potent inhibitor exhibiting