ライフサイエンスコーパス: gelonin

1 plant-derived ribosomal inactivating protein gelonin.

2 rders of magnitude lower than the unmodified gelonin.

3 ribosome inactivating protein toxins such as gelonin.

4 verexpress P-gp are also resistant to HuM195-gelonin.

5 h acts on precisely the same RNase target as gelonin.

6 ated the adenine DNA glycosylase activity of gelonin.

7 ) of 100 pM, comparable with that for native gelonin (104 pM).

8 Gelonin, a plant-derived toxin that inhibits protein tra

9 Gelonin, a type 1 ribosome-inactivating toxin, lacks cel

10 ed, when compared with administration of TAT-gelonin alone.

11 EGF, composed of the recombinant plant toxin gelonin and EGF.

12 When mixing together, TAT-gelonin and T84.66-Hep could associate tightly and autom

13 ve resistance to the immunotoxin (IT) HuM195-gelonin and to free rGelonin.

14 A re-examination of the classification of gelonin as a DNA glycosylase/AP lyase using the borohydr

15 ated the potential of utilizing CPP-modified gelonin as a highly potent anticancer drug to overcome l

16 specialized cytosolic delivery strategy for gelonin as an effective anti-tumor therapeutic agent.

17 rnalization of gelonin (via pinocytosis) and gelonin-based immunotoxins (via antigen-dependent, recep

18 Gelonin-based immunotoxins vary widely in their cytotoxi

19 might be utilized to improve the potency of gelonin-based immunotoxins.

20 Under these conditions, gelonin catalytically removed adenine from single-strand

21 We have used RNA aptamer:gelonin conjugates to target and specifically destroy ce

22 concentrations of unencapsulated gelonin or gelonin encapsulated alone in pH-sensitive liposomes exh

23 nability to internalize into cells, however, gelonin exerts only limited tumoricidal effect.

24 A chimeric TAT-gelonin fusion protein was genetically engineered, and i

25 Being a hydrophilic macromolecule, however, gelonin has limited access to its target subcellular com

26 000-fold enhanced cytotoxicity compared with gelonin (IC(50) approximately 0.25-30 nM versus 500 nM).

27 mal gelonin-mediated B16 cell killing with a gelonin IC50 of approximately 0.1 nM with an extreme eff

28 This anti-I-A(k) gelonin immunotoxin depletes I-A(k) expressing cells spe

29 We have synthesized novel gelonin immunotoxins using two different binding scaffol

30 get cells but also decreases the toxicity of gelonin in non-target cells.

31 bition of tumor growth (46%) without causing gelonin-induced systemic toxicity.

32 Gelonin is a type I plant toxin that has potential as an

33 Results confirmed that gelonin-LMWP chemical conjugate (cG-L) and recombinant g

34 WP chemical conjugate (cG-L) and recombinant gelonin-LMWP chimera (rG-L) possessed N-glycosidase acti

35 t gelonin (rGel); however, unlike rGel, both gelonin-LMWPs were able to internalize into cells.

36 capsulated listeriolysin O enabled liposomal gelonin-mediated B16 cell killing with a gelonin IC50 of

37 equivalent concentrations of unencapsulated gelonin or gelonin encapsulated alone in pH-sensitive li

38 Gelonin, pokeweed antiviral protein, and ricin, previous

39 nked by a flexible G(4)S tether to the toxin gelonin (rGel) and expressed this as a soluble protein i

40 We generated a construct of recombinant gelonin (rGel) fused to BLyS to specifically target quie

41 th a G(4)S tether to recombinant plant toxin gelonin (rGel) in an orthotopic glioblastoma mouse model

42 Fv e23 fused to the highly toxic recombinant gelonin (rGel) molecule.

43 ascular ablative agent VEGF(121)/recombinant gelonin (rGel) strongly inhibited tumor growth.

44 i-Her2/neu immunotoxins fused to recombinant gelonin (rGel) to the activity of bivalent IgG-containin

45 emical conjugate composed of the recombinant gelonin (rGel) toxin and the anti-Fn14 antibody ITEM-4 (

46 /kg VEGF/rGel, but not uncoupled recombinant gelonin (rGel), there was immunofluorescent staining for

47 sFv) and fused this to the recombinant toxin gelonin (rGel).

48 equivalent to that of unmodified recombinant gelonin (rGel); however, unlike rGel, both gelonin-LMWPs

49 ments suggested similar interactions between gelonin's DNA-binding domain and oligonucleotides with a

50 cacy studies also revealed that only the TAT-gelonin/T84.66-Hep complex yielded a significant inhibit

51 we describe an efficient means of delivering gelonin to the cytosol of B16 melanoma cells.

52 ficantly augmented (58-fold) delivery of TAT-gelonin to the tumor target was observed, when compared

53 protein (Fn14) antibody fused to recombinant gelonin toxin as a potential therapeutic agent (designat

54 n consisting of VEGF121 coupled to the toxin gelonin (VEGF/rGel).

55 ly potent yet cell-impermeable protein toxin gelonin via the aid of a cell-penetrating peptide (also

56 ow cytometry, we measured internalization of gelonin (via pinocytosis) and gelonin-based immunotoxins

57 come this cell membrane barrier, we modified gelonin, via both chemical conjugation and genetic recom

58 Gelonin was co-encapsulated inside pH-sensitive liposome

59 the borohydride trapping assay revealed that gelonin was similar to the DNA glycosylase MutY: both en

60 iposomes containing both listeriolysin O and gelonin were more effective than control formulations in

61 tx1 A chain, and the analogous plant protein gelonin, whereas the antibiotic did not show any protect

62 her characterized the interaction of the RIP gelonin with single-stranded oligonucleotides and invest