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1 e in development, including gene therapy and gene editing.
2  transfected with CRISPR-Cas9 components for gene editing.
3 xonuclease Xrn1 were prepared by CRISPR-Cas9 gene editing.
4 s a novel, attractive target for therapeutic gene editing.
5 cetolactate synthase1 gene through in planta gene editing.
6 oduced different isogenic iPS cell lines via gene editing.
7 e-specific nucleases into cells for targeted gene editing.
8 influence of strand bias on the mechanism of gene editing.
9 f the underlying mechanism used to carry out gene editing.
10 -directed repair regulates the SNGD-mediated gene editing.
11 romotes efficient nucleotide substitution by gene editing.
12 t palindromic repeats (CRISPR-Cas9)-mediated gene editing.
13 eep6 knockout mouse generated by CRISPR/Cas9 gene editing.
14  towards improving the safety and utility of gene editing.
15 the possible future clinical applications of gene editing.
16 ISPR/Cas9 system has brought in a new era of gene editing.
17 rget gene alone did not facilitate efficient gene editing.
18 /CRISPR-associated) homology-directed repair gene-editing.
19      We demonstrate that CRISPR/Cas-mediated gene editing allows the simultaneous disruption of five
20                                    Postnatal gene editing also enhanced skeletal muscle function, as
21 nd offers an effective adaptive strategy for gene editing and coevolution among interactive immune re
22 y platforms of tomorrow will require precise gene editing and delivery of entire complex pathways.
23  results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, a
24 f sgRNA activity to improve sgRNA design for gene editing and genetic screens.
25  Here, we used TALEN and CRISPR/Cas-mediated gene editing and hPSC-directed differentiation for a sys
26                                  CRISPR/Cas9 gene editing and imaging approaches show that loss of AK
27            For both CRISPR/Cas9-mediated HDR gene editing and indel mutation, the gene-edited F0 embr
28 d Cas9 ribonucleoprotein (Cas9 RNP)-mediated gene editing and lentiviral transduction to generate PD-
29 summary, SNGD promotes precise and efficient gene editing and may be a promising strategy for the dev
30 ces in living cells can be used for targeted gene editing and mutagenesis.
31  (CRC) and metastasis, which rely on in situ gene editing and orthotopic organoid transplantation in
32 erimental data on the use of CRISPR/Cas9 for gene editing and regulation, we implement a comprehensiv
33 gh relevance to the potential application of gene editing and stem-cell technologies for treating hum
34 iciently expressed and can mediate multiplex gene editing and sustained transcriptional activation in
35 HUH-tags as fusion partners in Cas9-mediated gene editing and the construction of doubly DNA-tethered
36 A and repair template DNA can induce precise gene editing and the correction of genetic diseases in a
37 the scope of applications in nanotechnology, gene editing, and DNA library construction.
38               Advances in chemical genomics, gene editing, and model systems now permit deconstructio
39 uce the time and cost of in vitro or ex vivo gene-editing applications in precision medicine and drug
40 tential advantages and problems with using a gene editing approach as a treatment for diseases caused
41               We then employed a CRISPR/Cas9 gene editing approach to delete the -10 and -30 kb Mmp13
42 gous reporter assays, we sought to develop a gene editing approach to investigate the regulatory acti
43 apy approaches to DMD under investigation, a gene editing approach using oligonucleotide vectors has
44                              Here, we used a gene-editing approach to generate a human COX18 knock-ou
45 this study, we developed and tested a direct gene-editing approach to induce exon deletion and recove
46  of this assay will facilitate comparison of gene editing approaches and their optimization, accelera
47                 Another potential target for gene editing are DNA viruses that cause chronic pathogen
48 n of HSCs, by either vector gene addition or gene editing, are facilitating successful treatments for
49                                  CRISPR/Cas9 gene editing as well as overexpression experiments revea
50              We also show that SNGD promotes gene editing at endogenous loci in human cells.
51          Here the authors show, using CRISPR gene editing, ATAC-seq and ChIP-seq, that specific Runx1
52 ention for its promise in basic research and gene editing-based therapeutics.
53 argely determines treatment frequency of non-gene-editing-based therapies targeting the cause of geno
54  editing in mammalian cells may well lead to gene editing becoming a novel treatment for a range of h
55 L in transgenic rice by CRISPR/Cas9-mediated gene editing blocked starch and protein accumulation, re
56    The CRISPR-Cas9 system has revolutionized gene editing both at single genes and in multiplexed los
57  standing inefficiencies in targeted nuclear gene editing broadly hinder Chlamydomonas research.
58 l sgRNA design tools have been developed for gene editing, but currently there is no tool for the des
59 length and tested their ability to stimulate gene editing by a donor oligonucleotide in human cells.
60                                              Gene editing by modified single-stranded oligonucleotide
61               Finally, we show how inducible gene editing can be achieved by combining the TAEL and C
62 ivo assay systems were developed to evaluate gene editing capabilities in C. elegans.
63             The work presented here explores gene editing capabilities in the model organism Caenorha
64            Importantly, the unique multiplex gene-editing capabilities of the CRISPR/Cas9 system faci
65 ation sequence), DNA recombination (Cre) and gene editing (Cas9).
66 d adeno-associated virus-9 (AAV9) to deliver gene-editing components to postnatal mdx mice, a model o
67                            CRISPR-Cas9 based gene editing confirmed that PfMDR1 point mutations media
68 pically used in other lab animals to deliver gene editing constructs have been less effective in song
69 ssess repair pathway usage in several common gene editing contexts confirms the importance that chrom
70           Cells with extremely low levels of gene editing could still be identified and isolated usin
71 s in G2/M with ABT phase increases on-target gene editing, defined as correct targeting cassette inte
72                                              Gene editing directed by modified single-stranded DNA ol
73  leads to an enhancement in the frequency of gene editing directed by single-stranded DNA oligonucleo
74 inally, using a CRISPR-Cas9-based method for gene editing directly in the mouse adult pancreas, we sh
75 or DNA has great potential for improving the gene editing efficiency of Cas9 and Cpf1, but has not be
76  (TALE) resulted in a further enhancement of gene editing efficiency.
77 donor plasmid backbone markedly improved the gene-editing efficiency.
78 short palindromic repeat/CRISPR-associated 9 gene editing elicited a dramatic increase in pigment con
79                                              Gene editing enables the site-specific modification of t
80 nes have reached clinical testing, including gene-editing enzymes, protein-based inhibitors, and RNA-
81 e V Cpf1 systems, and can facilitate precise gene editing even between identical genomic sites within
82 ates were evaluated, representing both minor gene editing events (restriction site insertion) to mimi
83 ains with varying concentrations of ODNs, no gene editing events were detected.
84 l biology; functional and comparative OMICs; gene editing; expanded use of model organisms; and a new
85  perform a posterior careful analysis of the gene editing experiment.
86 ression of more than one gRNAs for multiplex gene editing from a single pol II promoter.
87 emonstrate that AAV-mediated muscle-specific gene editing has significant potential for therapy of ne
88 g and direct transdifferentiation as well as gene editing have gradually become hot research topic; b
89 e cells from patients' human-induced PSCs by gene editing have opened up many potential gateways for
90 gularly interspaced palindromic repeat-based gene editing, human RBL(ralphaKO) cells were created tha
91 tion studies, confirmed by CRISPR-Cas9-based gene editing, identified the digestive vacuole membrane-
92 veral of which were confirmed by CRISPR/Cas9-gene editing in a different cell type.
93  system has proven to be a powerful tool for gene editing in a plethora of organisms.
94                 Despite the demonstration of gene editing in Chlamydomonas in 1995, the isolation of
95                   We report that CRISPR-Cas9 gene editing in combination with massively parallel olig
96                                    Following gene editing in DMD patient myoblasts, dystrophin expres
97 g the time to establish mutant lines, mosaic gene editing in founder animals, and low homologous reco
98 r (SCF)/c-Kit pathway yielded high levels of gene editing in haematopoietic stem cells (HSCs) in a mo
99 (HRas) with mVenus fluorescent protein using gene editing in HeLa cells.
100 et al. (2016) elegantly combine CRISPR-based gene editing in hESCs with directed beta cell differenti
101             The approach outlined here makes gene editing in hPSCs a more viable tool for disease mod
102 ustom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of
103                                 Here we used gene editing in human cells to create a chemical genetic
104  immunity in bacteria as a facile system for gene editing in mammalian cells may well lead to gene ed
105  CRISPR/Cas9 system allows sequence-specific gene editing in many organisms and holds promise as a to
106 ator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to pr
107 ch2, Smo, Shh and 7dhcr were inactivated via gene editing in multiple combinations, allowing us to me
108                 To establish CRISPR-directed gene editing in N. vitripennis, we targeted a conserved
109 ults demonstrate the feasibility of seamless gene editing in one-cell embryos to create genetic disea
110 ALEN) mRNA allows highly efficient multiplex gene editing in primary human T cells.
111 can be exceedingly costly for application of gene editing in primate species.
112 trating the high efficiency of Cas9-mediated gene editing in rats for simultaneous generation of comp
113 e an ability to achieve a high efficiency of gene editing in rhesus monkey zygotes, with no detected
114 e marker and allows sophisticated markerless gene editing in situ.
115  targeted mutagenesis, gene integration, and gene editing in soybean (Glycine max).
116 A-gRNA gene (PTG) strategy enables multiplex gene editing in the family of closely related mitogen-ac
117                     We have used CRISPR/Cas9 gene editing in the mouse to mutate the gene encoding eE
118 onal Cas9 expression and temporal control of gene editing in the presence of an FKBP12 synthetic liga
119  emerged as a robust technology for targeted gene editing in various organisms, including plants, whe
120 eased and serial dosing regimens for somatic gene editing in vivo.
121    Our techniques can produce high frequency gene editing in X. laevis, permitting analysis in the F0
122                                 Simultaneous gene editing in zygotes affords an efficient approach fo
123 her site-specific modification of the gene ("gene editing")--in this case, the infusion of autologous
124  Reduction of CIDEB protein levels by HCV or gene editing, in turn, leads to multiple aspects of lipi
125 , including gene overexpression, CRISPR/Cas9 gene editing, inducible technologies, optogenetic or DRE
126                                              Gene editing is a rapidly developing area of biotechnolo
127                               TALEN-mediated gene editing is a useful tool for dissecting the biology
128                                    Efficient gene editing is essential to fully utilize human pluripo
129                            It is likely that gene editing is facilitated by the direct incorporation
130                    The increase in on-target gene editing is locus-independent and specific to the ce
131 -CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentially an important tool for defini
132                                Cas9-mediated gene editing is simple, scalable, and rapid, and it can
133                               However, where gene editing is targeted to amplified loci, the resultin
134                                SNGD-mediated gene editing led to a markedly lower indel frequency tha
135                                              Gene editing mediated by oligonucleotides has been shown
136 e CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precis
137                              The CRISPR/Cas9 gene editing method is comprised of the guide RNA (gRNA)
138 velop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with
139              Our system builds upon existing gene editing methods in the thoroughness with which the
140 d alleles up to 18-fold higher than standard gene-editing methods, and enrich cell populations contai
141                              New methods for gene editing might allow additional PIDs to be treated b
142                   Disruption of Prod1 with a gene-editing nuclease abrogates these cells, and blocks
143                                              Gene-editing nucleases enable targeted modification of D
144  SimpleCell strategy using nuclease-mediated gene editing of a human cell line (MDA-MB-231) to reduce
145 ats (CRISPR) directed Cas9 nuclease-mediated gene editing of CXCR7 revealed that prostate cancer cell
146 us and cynomolgus zygotes leads to effective gene editing of MECP2 with no detected off-target mutage
147                                              Gene editing of MECP2(R306C) cells to generate isogenic
148 e and extracellular domain mutations through gene editing of the endogenous loci in HER2 nonamplified
149                 We used CRISPR/Cas9-mediated gene editing of the Ern1 locus to study the role of the
150             When reintroduced by CRISPR-Cas9 gene editing of wild-type cells, these mutations reverse
151                         CRISPR-Cas9-mediated gene-editing of the MBNL1-binding site within the Srf 3'
152 ing for 'directed mutagenesis' and targeted 'gene editing' of the plant and mammalian genome as well
153 edented flexibility that iPSC technology and gene editing offer academic and industry-based researche
154 the target site to evaluate the frequency of gene editing on extrachromosomal array transgenic lines.
155                                          The gene editing potential of CRISPR/Cas9 encapsulated by ZI
156                                 We optimized gene-editing protocols for several Chlamydomonas strains
157 lexes substantially and significantly reduce gene editing rates.
158 s, however, as well as its importance to the gene editing reaction itself, has yet to be elucidated i
159 fore low frequency events (like those in the gene editing reaction) may be detected.
160                    As the direct delivery of gene-editing reagents bypasses the use of transgenes, th
161 e human genome is challenging with non-viral gene-editing reagents, since most of the edited sequence
162 etter understanding of how the efficiency of gene editing relates to the target sequence will offer t
163 ine in zygotes, its application in postnatal gene editing remains incompletely characterized.
164 ile the mechanism of action of combinatorial gene editing remains to be elucidated, the regulatory ci
165 a prerequisite for mitochondrial CRISPR/Cas9 gene editing, remains controversial.
166                          Overall, single TCR gene editing represents a clinically feasible approach t
167               Mechanistically, SNGD-mediated gene editing requires long-sequence homology between the
168      Loss of CXCR7 expression by CRISPR-Cas9 gene editing resulted in a halt of cell proliferation, s
169                                              Gene editing revealed +9.5-like activity to mediate GATA
170                                  CRISPR-Cas9 gene editing revealed that both BTK and B lymphocyte kin
171 oach (for example, mutagenesis, CRISPR-based gene editing, RNA interference, morpholinos or pharmacol
172         Development of CRISPR/Cas9 transient gene editing screening tools in plant biology has been h
173 lopment as a tool has made sequence-specific gene editing several magnitudes easier.
174 hought, reinforcing the importance of the GP gene editing site for EBOV replication and pathogenicity
175 ther, these data support a phylogeny of sytI gene editing spanning more than 250 million years of hex
176 palindromic repeat (CRISPR)-caspase 9 (Cas9) gene-editing strategies.
177 and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of differe
178  and protocadherins, we used a combinatorial gene-editing strategy in multiple cell lines to evaluate
179 s, CRISPR/Cas9 technology represents a novel gene-editing strategy with compelling robustness, specif
180 ished transgenic cells can be used for other gene-editing studies and is well-suited for high-through
181 aced short palindromic repeats (CRISPR)/Cas9 gene editing system to determine how mutations affect EN
182 fibroblast (MEF) cells using the CRISPR/Cas9 gene editing system.
183                Here we present a CRISPR/Cas9 gene-editing system that combines Cas9 ribonucleoprotein
184 e used chromatin immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture
185            CRISPR/Cas9 system is an emerging gene-editing technique with the potential to eliminate o
186         It is rapidly replacing conventional gene editing techniques and has high utility for both ge
187 Here, we summarize the development of modern gene editing techniques and the biology of DSB repair on
188 thods of cell reprogramming, and using novel gene editing techniques for generating genetically corre
189                                              Gene editing techniques have been extensively used to at
190  reproductive biology and compatibility with gene editing techniques, which together have provided a
191 programming, along with CPP contributions to gene editing techniques.
192                  Capitalizing on CRISPR/Cas9 gene-editing techniques and super-resolution nanoscopy,
193                                              Gene editing technologies offer new options for developi
194 pluripotent stem cell (iPSC) technology, and gene editing technologies-including in particular the cl
195                                              Gene-editing technologies have made it feasible to creat
196 potency of approaches incorporating advanced gene-editing technologies into ACT protocols to silence
197                            The confluence of gene-editing technologies, induced pluripotent stem cell
198 venance of human biomaterials and the use of gene-editing technologies.
199                                        Using gene editing technology (CRISPR/Cas9), the SIRT1 gene wa
200                           Recent advances in gene editing technology have introduced the potential fo
201                         We developed the TCR gene editing technology that is based on the knockout of
202          Here the authors employ CRISPR/Cas9 gene editing technology to silence VEGFR2, a major regul
203                CRISPR/Cas is a revolutionary gene editing technology with wide-ranging utility.
204 anscription activator-like effector nuclease gene editing technology, we have knocked out both EVI an
205 epeats/CRISPR-associated gene9 (CRISPR/Cas9) gene editing technology.
206  cells, mutations might be repaired with new gene-editing technology based on the bacterial system of
207 ndromic repeats/CRISPR associated protein 9) gene-editing technology to transform Leu33 (+) megakaryo
208 quence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null
209 ISPR-Cas) allows more specific and efficient gene editing than all previous genetic engineering syste
210 ons, it is inappropriate to perform germline gene editing that culminates in human pregnancy.
211          We developed a strategy for precise gene editing that does not generate DSBs.
212 ome sequencing, genetic complementation, and gene editing, that haploid induction in maize (Zea mays)
213 of geminivirus-based vectors enables precise gene editing through homologous recombination.
214 Cell Stem Cell, Black et al. (2016) employed gene editing to activate endogenous loci of the reprogra
215 orskolin-induced swelling that is rescued by gene editing to correct the disease mutation.
216            Here we used CrispR-Cas9-mediated gene editing to delete the gene encoding for AC, ASAH1,
217 y RNAs to inhibit virus replication, we used gene editing to derive human cell lines that lack a func
218                                  Here we use gene editing to fluorescently tag endogenous IP3Rs, and
219 echnologies such as imaging, proteomics, and gene editing to generate a more precise understanding of
220 uent mutations in HGSC, and used CRISPR/Cas9 gene editing to generate derivatives with deletions in T
221                                 Here we used gene editing to generate mice expressing only activation
222 is proof-of-principle study, we used precise gene editing to induce Q(5)G substitution in both allele
223 We use adeno-associated virus (AAV)-mediated gene editing to knock in HLA-E genes at the B2M locus in
224                 We used CRISPR/Cas9-mediated gene editing to knockout the HMGB4 gene in a testicular
225               Therefore, we used CRISPR-Cas9 gene editing to modify the mouse genome to encode two am
226               Here, we have used CRISPR/Cas9 gene editing to mutate CTCF-binding sites at the putativ
227 e a compelling demonstration of the power of gene editing to rapidly improve yield traits in crop bre
228               We used RNA-guided CRISPR/Cas9 gene editing to specifically target for deletion of DNA
229                    With CRISPR/Cas9-mediated gene editing to stably knock out and recover Rab8a in ma
230                    Here we apply CRISPR-Cas9 gene editing to tag a cytoskeletal protein (alpha-tubuli
231 IA-PET) sequencing data, we used CRISPR-Cas9 gene editing to target three of the STAT5 binding sites
232 inally, we describe prospects for the use of gene editing to treat human genetic diseases.
233 RISPR/Cas9 has recently been introduced as a gene editing tool and shows considerable promise.
234                    CRISPR/Cas9 is a powerful gene editing tool for gene knockout studies and function
235 dings suggest that CRISPR/Cas9 is a powerful gene editing tool that can uncover novel mechanisms of c
236                        CRISPR is a versatile gene editing tool which has revolutionized genetic resea
237 ges and plasmids, as well as a revolutionary gene editing tool.
238            In this study, we used the latest gene-editing tool, CRISPR/Cas9 technology, combined with
239 f vectors provide an organized comprehensive gene editing toolbox of considerable scientific value.
240               We have expanded the livestock gene editing toolbox to include transcription activator-
241 , providing the basis for gene therapy using gene editing tools.
242    In the postgenomic era, sequence-specific gene-editing tools enable the functional analysis of gen
243 aptive immunity in prokaryotes and promising gene-editing tools from bacteria to humans.
244                                       Recent gene-editing tools, such as transcription activator-like
245             By combining two newly developed gene-editing tools, the TALEN and CRISPR/Cas systems, we
246 ffect of postnatal CRISPR/Cas9-based cardiac gene editing using adeno-associated virus serotype 9 to
247 d protein expressions as well as single-cell gene editing using clustered regularly interspaced short
248 easibility of highly specific clonal ex vivo gene editing using CRISPR/Cas9 and highlights the value
249                                        Thus, gene editing using CRISPR/Cas9 may provide a new therape
250                            The advantages of gene editing versus gene targeting in embryonic stem cel
251 P in Nicotiana benthamiana (16c) plants, and gene editing was accompanied by loss of GFP expression.
252                                  Although no gene editing was detected in the miR-497a genomic locus,
253 duced loss of heterozygosity, and indeed Apc gene editing was less efficient in tetraploid than in di
254                                  CRISPR/Cas9 gene editing was used to knock out pig conceptus IL1B2 e
255            Furthermore, CRISPR-Cas9-mediated gene editing was used to produce Reep6 knock-in mice wit
256                            Using CRISPR/Cas9 gene editing, we modeled HGSC by generating novel ID8 de
257 g kinase inhibitors and CRISPR/Cas9-mediated gene editing, we show here that triple-negative but not
258                                     By using gene editing, we show that C. elegans and C. briggsae he
259                                 HIV provirus gene-editing were confirmed by cell genomic DNA PCR and
260 ls to support homologous recombination-based gene editing will be presented.
261                  Here we combine CRISPR/Cas9 gene editing with an innovative high-throughput genotypi
262                     In the future, combining gene editing with CRISPR/Cas9 and PSCs complementation c
263                                              Gene editing with engineered nucleases enables site-spec
264 l review this RNA-guided nuclease system for gene editing with respect to its usefulness for cardiova
265               Here, we extend our studies of gene editing with self-pairing, chimeric RNA/DNA oligonu
266                                              Gene editing with zinc finger nucleases, transcription a
267 tem to achieve clinically relevant levels of gene editing without off-target effects.

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