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1 e in development, including gene therapy and gene editing.
2 transfected with CRISPR-Cas9 components for gene editing.
3 xonuclease Xrn1 were prepared by CRISPR-Cas9 gene editing.
4 s a novel, attractive target for therapeutic gene editing.
5 cetolactate synthase1 gene through in planta gene editing.
6 oduced different isogenic iPS cell lines via gene editing.
7 e-specific nucleases into cells for targeted gene editing.
8 influence of strand bias on the mechanism of gene editing.
9 f the underlying mechanism used to carry out gene editing.
10 -directed repair regulates the SNGD-mediated gene editing.
11 romotes efficient nucleotide substitution by gene editing.
12 t palindromic repeats (CRISPR-Cas9)-mediated gene editing.
13 eep6 knockout mouse generated by CRISPR/Cas9 gene editing.
14 towards improving the safety and utility of gene editing.
15 the possible future clinical applications of gene editing.
16 ISPR/Cas9 system has brought in a new era of gene editing.
17 rget gene alone did not facilitate efficient gene editing.
18 /CRISPR-associated) homology-directed repair gene-editing.
21 nd offers an effective adaptive strategy for gene editing and coevolution among interactive immune re
22 y platforms of tomorrow will require precise gene editing and delivery of entire complex pathways.
23 results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, a
25 Here, we used TALEN and CRISPR/Cas-mediated gene editing and hPSC-directed differentiation for a sys
28 d Cas9 ribonucleoprotein (Cas9 RNP)-mediated gene editing and lentiviral transduction to generate PD-
29 summary, SNGD promotes precise and efficient gene editing and may be a promising strategy for the dev
31 (CRC) and metastasis, which rely on in situ gene editing and orthotopic organoid transplantation in
32 erimental data on the use of CRISPR/Cas9 for gene editing and regulation, we implement a comprehensiv
33 gh relevance to the potential application of gene editing and stem-cell technologies for treating hum
34 iciently expressed and can mediate multiplex gene editing and sustained transcriptional activation in
35 HUH-tags as fusion partners in Cas9-mediated gene editing and the construction of doubly DNA-tethered
36 A and repair template DNA can induce precise gene editing and the correction of genetic diseases in a
39 uce the time and cost of in vitro or ex vivo gene-editing applications in precision medicine and drug
40 tential advantages and problems with using a gene editing approach as a treatment for diseases caused
42 gous reporter assays, we sought to develop a gene editing approach to investigate the regulatory acti
43 apy approaches to DMD under investigation, a gene editing approach using oligonucleotide vectors has
45 this study, we developed and tested a direct gene-editing approach to induce exon deletion and recove
46 of this assay will facilitate comparison of gene editing approaches and their optimization, accelera
48 n of HSCs, by either vector gene addition or gene editing, are facilitating successful treatments for
53 argely determines treatment frequency of non-gene-editing-based therapies targeting the cause of geno
54 editing in mammalian cells may well lead to gene editing becoming a novel treatment for a range of h
55 L in transgenic rice by CRISPR/Cas9-mediated gene editing blocked starch and protein accumulation, re
56 The CRISPR-Cas9 system has revolutionized gene editing both at single genes and in multiplexed los
58 l sgRNA design tools have been developed for gene editing, but currently there is no tool for the des
59 length and tested their ability to stimulate gene editing by a donor oligonucleotide in human cells.
66 d adeno-associated virus-9 (AAV9) to deliver gene-editing components to postnatal mdx mice, a model o
68 pically used in other lab animals to deliver gene editing constructs have been less effective in song
69 ssess repair pathway usage in several common gene editing contexts confirms the importance that chrom
71 s in G2/M with ABT phase increases on-target gene editing, defined as correct targeting cassette inte
73 leads to an enhancement in the frequency of gene editing directed by single-stranded DNA oligonucleo
74 inally, using a CRISPR-Cas9-based method for gene editing directly in the mouse adult pancreas, we sh
75 or DNA has great potential for improving the gene editing efficiency of Cas9 and Cpf1, but has not be
78 short palindromic repeat/CRISPR-associated 9 gene editing elicited a dramatic increase in pigment con
80 nes have reached clinical testing, including gene-editing enzymes, protein-based inhibitors, and RNA-
81 e V Cpf1 systems, and can facilitate precise gene editing even between identical genomic sites within
82 ates were evaluated, representing both minor gene editing events (restriction site insertion) to mimi
84 l biology; functional and comparative OMICs; gene editing; expanded use of model organisms; and a new
87 emonstrate that AAV-mediated muscle-specific gene editing has significant potential for therapy of ne
88 g and direct transdifferentiation as well as gene editing have gradually become hot research topic; b
89 e cells from patients' human-induced PSCs by gene editing have opened up many potential gateways for
90 gularly interspaced palindromic repeat-based gene editing, human RBL(ralphaKO) cells were created tha
91 tion studies, confirmed by CRISPR-Cas9-based gene editing, identified the digestive vacuole membrane-
97 g the time to establish mutant lines, mosaic gene editing in founder animals, and low homologous reco
98 r (SCF)/c-Kit pathway yielded high levels of gene editing in haematopoietic stem cells (HSCs) in a mo
100 et al. (2016) elegantly combine CRISPR-based gene editing in hESCs with directed beta cell differenti
102 ustom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of
104 immunity in bacteria as a facile system for gene editing in mammalian cells may well lead to gene ed
105 CRISPR/Cas9 system allows sequence-specific gene editing in many organisms and holds promise as a to
106 ator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to pr
107 ch2, Smo, Shh and 7dhcr were inactivated via gene editing in multiple combinations, allowing us to me
109 ults demonstrate the feasibility of seamless gene editing in one-cell embryos to create genetic disea
112 trating the high efficiency of Cas9-mediated gene editing in rats for simultaneous generation of comp
113 e an ability to achieve a high efficiency of gene editing in rhesus monkey zygotes, with no detected
116 A-gRNA gene (PTG) strategy enables multiplex gene editing in the family of closely related mitogen-ac
118 onal Cas9 expression and temporal control of gene editing in the presence of an FKBP12 synthetic liga
119 emerged as a robust technology for targeted gene editing in various organisms, including plants, whe
121 Our techniques can produce high frequency gene editing in X. laevis, permitting analysis in the F0
123 her site-specific modification of the gene ("gene editing")--in this case, the infusion of autologous
124 Reduction of CIDEB protein levels by HCV or gene editing, in turn, leads to multiple aspects of lipi
125 , including gene overexpression, CRISPR/Cas9 gene editing, inducible technologies, optogenetic or DRE
131 -CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentially an important tool for defini
136 e CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precis
138 velop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with
140 d alleles up to 18-fold higher than standard gene-editing methods, and enrich cell populations contai
144 SimpleCell strategy using nuclease-mediated gene editing of a human cell line (MDA-MB-231) to reduce
145 ats (CRISPR) directed Cas9 nuclease-mediated gene editing of CXCR7 revealed that prostate cancer cell
146 us and cynomolgus zygotes leads to effective gene editing of MECP2 with no detected off-target mutage
148 e and extracellular domain mutations through gene editing of the endogenous loci in HER2 nonamplified
152 ing for 'directed mutagenesis' and targeted 'gene editing' of the plant and mammalian genome as well
153 edented flexibility that iPSC technology and gene editing offer academic and industry-based researche
154 the target site to evaluate the frequency of gene editing on extrachromosomal array transgenic lines.
158 s, however, as well as its importance to the gene editing reaction itself, has yet to be elucidated i
161 e human genome is challenging with non-viral gene-editing reagents, since most of the edited sequence
162 etter understanding of how the efficiency of gene editing relates to the target sequence will offer t
164 ile the mechanism of action of combinatorial gene editing remains to be elucidated, the regulatory ci
168 Loss of CXCR7 expression by CRISPR-Cas9 gene editing resulted in a halt of cell proliferation, s
171 oach (for example, mutagenesis, CRISPR-based gene editing, RNA interference, morpholinos or pharmacol
174 hought, reinforcing the importance of the GP gene editing site for EBOV replication and pathogenicity
175 ther, these data support a phylogeny of sytI gene editing spanning more than 250 million years of hex
177 and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of differe
178 and protocadherins, we used a combinatorial gene-editing strategy in multiple cell lines to evaluate
179 s, CRISPR/Cas9 technology represents a novel gene-editing strategy with compelling robustness, specif
180 ished transgenic cells can be used for other gene-editing studies and is well-suited for high-through
181 aced short palindromic repeats (CRISPR)/Cas9 gene editing system to determine how mutations affect EN
184 e used chromatin immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture
187 Here, we summarize the development of modern gene editing techniques and the biology of DSB repair on
188 thods of cell reprogramming, and using novel gene editing techniques for generating genetically corre
190 reproductive biology and compatibility with gene editing techniques, which together have provided a
194 pluripotent stem cell (iPSC) technology, and gene editing technologies-including in particular the cl
196 potency of approaches incorporating advanced gene-editing technologies into ACT protocols to silence
204 anscription activator-like effector nuclease gene editing technology, we have knocked out both EVI an
206 cells, mutations might be repaired with new gene-editing technology based on the bacterial system of
207 ndromic repeats/CRISPR associated protein 9) gene-editing technology to transform Leu33 (+) megakaryo
208 quence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null
209 ISPR-Cas) allows more specific and efficient gene editing than all previous genetic engineering syste
212 ome sequencing, genetic complementation, and gene editing, that haploid induction in maize (Zea mays)
214 Cell Stem Cell, Black et al. (2016) employed gene editing to activate endogenous loci of the reprogra
217 y RNAs to inhibit virus replication, we used gene editing to derive human cell lines that lack a func
219 echnologies such as imaging, proteomics, and gene editing to generate a more precise understanding of
220 uent mutations in HGSC, and used CRISPR/Cas9 gene editing to generate derivatives with deletions in T
222 is proof-of-principle study, we used precise gene editing to induce Q(5)G substitution in both allele
223 We use adeno-associated virus (AAV)-mediated gene editing to knock in HLA-E genes at the B2M locus in
227 e a compelling demonstration of the power of gene editing to rapidly improve yield traits in crop bre
231 IA-PET) sequencing data, we used CRISPR-Cas9 gene editing to target three of the STAT5 binding sites
235 dings suggest that CRISPR/Cas9 is a powerful gene editing tool that can uncover novel mechanisms of c
239 f vectors provide an organized comprehensive gene editing toolbox of considerable scientific value.
242 In the postgenomic era, sequence-specific gene-editing tools enable the functional analysis of gen
246 ffect of postnatal CRISPR/Cas9-based cardiac gene editing using adeno-associated virus serotype 9 to
247 d protein expressions as well as single-cell gene editing using clustered regularly interspaced short
248 easibility of highly specific clonal ex vivo gene editing using CRISPR/Cas9 and highlights the value
251 P in Nicotiana benthamiana (16c) plants, and gene editing was accompanied by loss of GFP expression.
253 duced loss of heterozygosity, and indeed Apc gene editing was less efficient in tetraploid than in di
257 g kinase inhibitors and CRISPR/Cas9-mediated gene editing, we show here that triple-negative but not
264 l review this RNA-guided nuclease system for gene editing with respect to its usefulness for cardiova
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