ライフサイエンスコーパス: gene gun

1 ures such as particle-mediated DNA delivery (gene gun).

2 and skin by particle-mediated gene transfer (Gene Gun).

3 293 cells and whole brain using a hand-held gene gun.

4 d organotypic brain slices using a hand-held gene gun.

5 DNA vaccines administered intradermally via gene gun.

6 ary vaccine Ags after single immunization by gene gun.

7 s only in sera of mice inoculated by using a gene gun.

8 rabbit Peyer's patches using a helium-driven gene gun.

9 parate plasmid after codelivery into skin by gene gun.

10 We reasoned that the gene gun administration of CIITA DNA with DNA vaccines e

11 ediated biolistic delivery using a hand-held gene gun, allows non-toxic labeling of multiple cells in

12 An N586-82Q plasmid, delivered via gene gun, also showed ELISA responses, mainly in the zQ1

13 nts were delivered to the skin of a mouse by gene gun and IFN-gamma levels were measured at the skin

14 Two delivery systems, cDNA delivered by gene gun and Venezuelan equine encephalitis virus-like r

15 entation may be more significant in clinical Gene Gun applications than in pre-clinical mouse studies

16 We conclude that transfected cells in gene gun-bombarded skin, but not needle-injected muscle,

17 psy of the skin target site up to 24 h after gene gun bombardment completely abrogated the Ab respons

18 been studied, including liposomal delivery, gene gun bombardment, and picoinjection into single livi

19 Following gene gun bombardments of DNA, both were biased towards t

20 The studies revealed that gene gun bombardments of DNAs expressing secreted Ags st

21 Following a gene gun booster, the presence of ASC in the inguinal ly

22 X3C-mut were inoculated into mice by using a gene gun, both plasmids elicited an antibody response de

23 ression vectors simultaneously delivered via gene gun can significantly augment antitumor effects, ev

24 Using the Biolistics Gene Gun, cDNA constructs encoding a Kv1 subfamily-speci

25 Using the Biolistics Gene Gun, cDNA constructs encoding either Kv2.1DN or Kv2

26 Using the biolistic gene gun, cDNAs encoding a dominant negative mutant Kv4.

27 Using the biolistic gene gun, cDNAs encoding a mutant Kv4.2 alpha subunit (K

28 was conducted to investigate whether Accell gene gun coadministration of DNA encoding human interleu

29 The use of the gene gun, combined with the new generation of fluorescen

30 vered by a combination of vaccinia virus and gene gun-delivered DNA were effective against SHIV89.6P

31 Gene gun-delivered plasmid encoding the wild-type NY-ESO

32 erologous prime/boost strategy consisting of gene gun-delivered PSCA-cDNA followed by Venezuelan equi

33 IL-4-producing CD4(+) T cells was found for gene gun deliveries of cell-associated Ags.

34 y raised immunoglobulin G2a (IgG2a), whereas gene gun deliveries of DNA predominantly raised IgG1.

35 Gene gun deliveries of secreted Ags were used to bias re

36 DNA vaccine efficacy by immunizing mice via gene gun delivery and challenging them with live, virule

37 e data suggested that muscle inoculation and gene gun delivery elicited Th1-like and Th2-like respons

38 Each regimen included gene gun delivery of a DNA vaccine expressing all SHIV89

39 Here we show that gene gun delivery of DNA encoding an H5 HA protein confe

40 ually given by intramuscular injection or by gene gun delivery of DNA-coated particles into the epide

41 all, the high levels of protection seen with gene gun delivery of HA-DNA were as good as, if not bett

42 Immunization of mice by Gene Gun delivery of the 47-kDa domain of SERA elicited

43 e evaluated a physical gene transfer method (gene gun delivery) for its ability to transfect the epid

44 tor-mediated gene transfers were superior to gene-gun delivery of plasmid DNA.

45 Cynos administered vaccine by gene gun developed antibodies to HEV (anti-HEV), whereas

46 response with mostly IgG2a anti-H1 Ab, while gene gun DNA immunization produced a predominantly Th2 r

47 ences in the doses of DNA used in saline and gene gun DNA immunization.

48 on site tissue for i.m. needle and epidermal gene gun DNA immunizations.

49 Intradermal vaccination by gene gun efficiently delivers DNA vaccines into DCs of t

50 In the i.m.-gene gun (g.g.)-g.g. regimen, the mice immunized with gp

51 ersely, intradermal genetic immunization via gene gun (GG) delivered TcPRAC as an immunogen, generati

52 Administration of DNA vaccines via gene gun has emerged as an important form of Ag-specific

53 Single plasmid immunization by gene gun identified a novel vaccine target antigen which

54 when these mice were challenged with OVA by gene gun immunization in the contraction phase of the pr

55 Gene gun immunization of dual-expression DNA vaccines in

56 ne injection of DNA into skin or muscle, and gene gun immunization of skin or muscle.

57 hin the epidermis impacts on the efficacy of Gene Gun immunization protocols.

58 Carefully timed gene gun immunization studies designed to take advantage

59 We tested the ability of intramuscular and gene gun immunization with DNA expressing influenza viru

60 ed in predominantly IgG2a responses, whereas gene gun immunization yielded a preponderance of IgG1 an

61 ss-presentation in CD8+ T cell priming using gene gun immunization, and indicate that augmentation of

62 in the generation of immune responses after gene gun immunization.

63 of protective responses from those seen with gene gun immunization.

64 .m.) injection or delivered intradermally by Gene Gun immunization.

65 For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted

66 cited to the SERA protein following i.m. and Gene Gun immunizations with SERA plasmid DNA was immunog

67 itro skin organ cultures were developed from gene gun immunized mouse ear specimens to obtain LC.

68 Waning gamma interferon production in gene gun-immunized animals was countered by a marked inc

69 Particle-mediated (gene gun) in vivo delivery of the murine interleukin 12

70 ata of in vivo transfection in the rabbit by Gene Gun indicate that reporter gene constructs containi

71 d particles to neuronal preparations with a "gene gun," individual neurons and glia whose membranes a

72 ccinations administered to the skin with the gene gun induced and progressively increased p11C, C-->M

73 epidermis using a hand-held, helium-driven "gene gun" induced beta-gal-specific Ab and lytic respons

74 due to cell respiration were monitored, with gene gun injected PEBBLEs, in rat C6 glioma cells.

75 ch remained viable after PEBBLE delivery via gene gun injection.

76 her gp120 or gp140 DNA vaccines delivered by gene gun inoculation followed by recombinant gp120 prote

77 body responses in mice immunized by a single gene gun inoculation of plasmid expressing the influenza

78 mbardment of DNA-coated gold beads by Accell gene gun inoculation.

79 For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inocula

80 Three received only gene gun inoculations.

81 lated intravenously, intramuscularly, and by gene gun inoculations.

82 intramuscular, intradermal, and epithelial (gene gun) inoculations.

83 accines were delivered to mice by biolistic (gene gun) intradermal inoculation.

84 Peritumoral gene gun introduction of interleukin-12 may be a novel,

85 at DNA vaccine with HEV ORF2 administered by gene gun is protective against a heterologous viral chal

86 DNA-based immunizations administered by the gene gun may be an effective method of inducing local im

87 vaccination and demonstrates the efficacy of gene gun-mediated delivery of foreign DNA to a mucosal t

88 Gene gun-mediated DNA vaccination stimulates an immune r

89 These results indicate that gene gun-mediated immunization with papillomavirus early

90 These results suggest that gene gun-mediated in vivo delivery of IL-12 cDNA should

91 Rabbits were then immunized by gene gun-mediated intracutaneous administration of four

92 Gene gun-mediated transfection of mouse inner ear sensor

93 Here we show that gene gun-mediated vaccination with an epitope-based DNA

94 y at the skin site receiving DNA transfer by gene gun on days 6, 8, 10, and 12 after tumor implantati

95 The hand-held gene gun provides a rapid and efficient method of incorp

96 radermal administration of nucleic acids via gene gun represents an efficient method for delivering n

97 However, gene gun responses are more difficult to categorize beca

98 odes, but not in other lymph nodes, revealed gene gun responses being initiated in the nodes that dra

99 er 4 doses of DNA administered by epidermal (gene gun) route, all guinea pigs developed enzyme-linked

100 munized via the intramuscular and epidermal (gene gun) routes with 100 and 1 micrograms, respectively

101 be easily performed using the Bio-Rad Helios gene gun system.

102 In addition, the corneal gene gun technique may be generally used to study the in

103 with a model DNA-based antigen delivered by gene gun technology would induce an antibody response de

104 Using gene gun technology, we immunized a Wistar rat with an A

105 ow that after immunization of mouse skins by gene gun, the number of antigen-bearing DCs that migrate

106 Gene gun therapy with interleukin-12 cDNA into the perit

107 ermally with high expresser plasmid, using a gene gun to administer three doses at 6-wk intervals.

108 We used the gene gun to deliver transgenic (tg) Ag to the skin of C5

109 ted with DNA-coated gold micro-projectiles ("Gene Gun") to induce potent cellular and humoral immune

110 In this study, we demonstrated that gene gun transfection and production of semaphorin III i

111 cial role in Purkinje cell development using gene gun transfections within intact murine cerebellar c

112 Gene gun treatment markedly augmented (3-fold) LC migrat

113 el this mechanism, we examined the impact of gene gun treatment on LC with respect to their activatio

114 we compared immune responses generated after gene gun vaccination of mice with DNA vaccine plasmids d

115 this model, depigmentation was introduced by gene gun vaccination with eukaryotic expression plasmids

116 nucleoprotein (NP) in C57BL/6 mice following gene gun vaccination.

117 A gene gun was used to inoculate vaccines into the epiderm

118 ntrast, cutaneous DC transfected in situ via gene gun were detected in the draining lymph node at a 2

119 Rhesus macaques vaccinated by gene gun with pWRG/AND-M developed remarkably high level