ライフサイエンスコーパス: gene knockdown

1 were generated through small interfering RNA gene knockdown.

2 n and the pleiotropic effects of EB1 and APC gene knockdown.

3 analysis using antisense morpholino-mediated gene knockdown.

4 ocytes in the brain, resulting in a specific gene knockdown.

5 s the most common LCA mutation, was used for gene knockdown.

6 ipts with altered expression following CLOCK gene knockdown.

7 the cross-linker are critical for efficient gene knockdown.

8 ese cells with double stranded (ds) RNAs for gene knockdown.

9 l molecule treatment and morpholino-mediated gene knockdown.

10 old nanoparticles, we demonstrated a tunable gene knockdown.

11 overexpression and RNA interference-mediated gene knockdown.

12 to observe significant downstream effects of gene knockdown.

13 d allows for a highly controlled approach to gene knockdown.

14 ing roles of RIP1 and RIP3 were confirmed by gene knockdown.

15 ects of multi-gene families via simultaneous gene knockdown.

16 sfection agents and are capable of efficient gene knockdown.

17 mes presents a powerful method for efficient gene knockdown.

18 logical antagonism, and local viral-mediated gene knockdown.

19 LY2228820 dimesylate treatment and by shRNA gene knockdown.

20 l analyses, physiology, genetic mapping, and gene knockdowns.

21 notypes seen using morpholino-based targeted gene knockdowns.

22 ormed transgenics to assess the phenotype of gene knockdowns.

23 TRPM7 gene knockdown abolished the promotion of bone-related g

24 th a nephrocyte-specific driver for targeted gene knockdown, allowing the identification of genes req

25 Compound jagged and notch gene knockdowns alter zebrafish biliary, kidney, pancrea

26 ating gene expression with morpholino-driven gene knockdown and capped RNA-mediated rescue, we show t

27 own to be dependent on Akt signaling by both gene knockdown and chemical inhibition methods.

28 By using gene knockdown and knockout approaches, we demonstrate t

29 Using in vitro RNAi-based gene knockdown and KO mice, we demonstrated that a stron

30 Through stable gene knockdown and mouse subcutaneous xenograft studies,

31 er, an increase likely related to the target gene knockdown and not a general effect of virus infecti

32 Gene knockdown and overexpression approaches show that r

33 an Th17 cell differentiation using both IRF4 gene knockdown and overexpression experiments.

34 ions of hepatic Atg14, we have performed the gene knockdown and overexpression in the mouse livers.

35 Gene knockdown and overexpression studies indicated that

36 Gene knockdown and overexpression studies suggest that O

37 Furthermore, both gene knockdown and pharmacological inhibition studies sh

38 ibrotic tissue facilitated sequence-specific gene knockdown and prevented fibrosis progression.

39 perplexed mutation and this was confirmed by gene knockdown and pyrimidine rescue experiments.

40 Through gene knockdown and rescue approaches, we also find that

41 Short interfering RNA (siRNA)-directed gene knockdown and rescue of tTLL expression demonstrate

42 A cells, an observation confirmed further by gene knockdown and selective inhibitors.

43 idenced by the results of antisense-mediated gene knockdown and the use of the furin- and PC6-selecti

44 f structure-activity relationships involving gene knockdown and toxicity.

45 tradiol suppressed apoptosis induced by both gene knockdowns and BEZ235 treatment.

46 ations of Caenorhabditis elegans: we induced gene knockdowns and used quantitative genetic methodolog

47 Chitin synthase gene knockdowns and various histochemical experiments in

48 es of splicing events were affected by dnmt3 gene knockdown, and change in two types, exon skipping a

49 dominant negative mutant, genetic knockout, gene knockdown, and chromatin immunoprecipitation approa

50 ion by pathway specific inhibitors, selected gene knockdown, and in vivo tumorigenicity assay.

51 major hurdle in gene therapy or therapeutic gene knockdown, and the development of intelligent and s

52 These methods achieve desirable levels of gene knockdown, and thus can be compared with methods de

53 progenitors, the effect of various reported gene knockdowns, and the reprogramming of pre-B cells in

54 We use a temporally-selective gene knockdown approach to identify endogenous functions

55 In this study, a PURB-specific gene knockdown approach was used in conjunction with bio

56 Using a gene knockdown approach, a genetic framework for PEO dev

57 ion of NF-kappaB signaling events by using a gene knockdown approach: RNA interference through delive

58 Gene knockdown approaches and transgenesis-based lineage

59 Indeed, complementary robo4 gene knockdown approaches in zebrafish embryos show lowe

60 Using a panel of inhibitors and gene knockdown approaches, we identified the upstream ki

61 Eve1 fused to the Gal4 activation domain and gene-knockdown approaches, we investigated the role of e

62 ERbeta gene knockdown, as well as coexpression of the dominant

63 Deletion mutation and gene knockdown assays revealed that formation of a funct

64 Furthermore, we observed that caspase 3 gene knockdown attenuated the growth-stimulating effect

65 deacetylase by small molecule inhibitors or gene knockdown blocks acquisition of BCR-ABL mutations a

66 l-defined nanoparticles and induce efficient gene knockdown both in vitro and in vivo.

67 ls capable of both cellular transfection and gene knockdown, but thus far are promiscuous structures,

68 Gene knockdown by RNA interference (RNAi) in Caenorhabdi

69 In addition, Rspo2 and Rspo3 gene knockdown by RNA interference significantly comprom

70 ive or inducible protein expression studies, gene knockdown by RNA interference, or affinity purifica

71 neered a human monocytic cell line for NALP1 gene knockdown by RNA interference.

72 MCN over-expression using adenovirus or EMCN gene knockdown by siRNA.

73 ntagonists were abolished following receptor gene knockdown by siRNA.

74 es performed in mice revealed that myoferlin gene knockdown can attenuate cancer cell proliferation i

75 RNA interference-mediated gene knockdown can be exploited to study the reprogramme

76 We show that RNAi-induced gene knockdown can be generated through introducing smal

77 Overall, the tunable properties and gene-knockdown capabilities of NanoScript enables its ut

78 transfection agents, and demonstrate a high gene knockdown capability in a cell model.

79 in length and have sequence-homology-driven gene-knockdown capability.

80 ression was significantly decreased in PAR-2 gene knockdown cells, whereas no change was detected in

81 024, respectively), and was blocked in PAR-2 gene knockdown cells.

82 lls, whereas no change was detected in PAR-1 gene knockdown cells.

83 a constitutively active or RNA interference gene knockdown construct, respectively, OsCDPK1 was foun

84 allowing combinatorial gene overexpression, gene knockdown, Cre-mediated gene deletion, or CRISPR/Ca

85 GSK-3beta inhibition and siRNA gene knockdown decreased bupivacaine induced cell death

86 Specific gene knockdown demonstrates that loss of loxl1 results i

87 ctional studies, but they are ineffective in gene knockdown during oogenesis, an important model syst

88 t significantly enhanced cellular uptake and gene knockdown efficiencies in adipose derived mesenchym

89 static interactions, which greatly limit the gene knockdown efficiency.

90 yl-1H-pyrazole -3-carboxamide [AM251] or CB1 gene knockdown) enhanced hMMC degranulation and increase

91 siRNA is a highly specific tool for targeted gene knockdown, establishing siRNA-mediated gene silenci

92 Host gene knockdown events did not affect virus antigenicity,

93 ence (RNAi) screen was performed to identify gene knockdown events that enhanced poliovirus replicati

94 Gene knockdown experiments and transplantation assays de

95 dbrain also arise from neural crest, we used gene knockdown experiments in zebrafish to disrupt neura

96 Gene knockdown experiments showed that an expression of

97 Temporally restricted gene knockdown experiments suggest that Lsd1 functions b

98 ized in all RNA interference (RNAi)-mediated gene knockdown experiments to ensure sound conclusions a

99 nd cooperated in carcinogenesis according to gene knockdown experiments.

100 de transgenic RNAi screen through ubiquitous gene knockdowns, focusing on regulators of adult Drosoph

101 kidney 293T cells undergoing HGPRT-specific gene knockdown followed by influenza virus ribonucleopro

102 properties resulted in an enhanced in vitro gene knockdown for the acid-degradable cationic nanopart

103 n of endogenous Gsc functions in MO-mediated gene knockdown frog and knockout mouse embryos unearthed

104 t repair capacity and the ability to perform gene knockdown in a high-throughput manner.

105 ion, this approach allowed inducible in vivo gene knockdown in any cell type that developed from this

106 of an aptamer fused to an siRNA for targeted gene knockdown in cells bearing an aptamer-binding recep

107 s of their uptake, and significantly greater gene knockdown in cells overexpressing the target antige

108 by evaluating the effect of MKK-4 and MKK-7 gene knockdown in cultured FLS.

109 C8ORF37 in a vertebrate system, we performed gene knockdown in Danio rerio and assessed the cardinal

110 RNAi-mediated gene knockdown in Drosophila melanogaster is a powerful

111 lencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells.

112 w that lipidoid nanoparticles mediate potent gene knockdown in hepatocytes and immune cell population

113 lockade of GATA3 using small interfering RNA gene knockdown in MCF-7 cells triggered fibroblastic tra

114 our that are broadly functional for targeted gene knockdown in mycobacteria.

115 ods of lentiviral short hairpin RNA-mediated gene knockdown in primary CD4 T cells, we identify inter

116 This capacity, combined with high-throughput gene knockdown in Stentor, enables time-course mechanist

117 erapeutics, which show efficient and durable gene knockdown in the liver, with signs of promising cli

118 he ability to produce a high level of target gene knockdown in the lung with minimal toxicity demonst

119 n vivo by antisense oligonucleotide-mediated gene knockdown in the medial prefrontal cortex, followed

120 2 (Pk2), and a central BBS gene, Bbs7, using gene knockdown in the zebrafish.

121 Finally, we compared transient gene knockdown in tissue culture with results in stable

122 The task of specific gene knockdown in vitro has been facilitated through the

123 mental system enabled induction of efficient gene knockdown in vivo without subsequent manipulation.

124 y to carry out transgenesis in X. laevis and gene knockdown in X. tropicalis, we demonstrate that end

125 Using a combination of in vivo gene knockdown in zebrafish and in vitro assays in human

126 ome-scale short hairpin RNA (shRNA)-mediated gene knockdowns in KIT-mutant GIST-T1 and GIST882.

127 The efficiency of gene knockdown increased as the number of lipid tails co

128 In contrast, MyoD gene knockdown increases cell survival of wild-type myob

129 To address the question of whether bag3 gene knockdown induces myofibrillar disorganization caus

130 Furthermore, Rsf-1 gene knockdown inhibited cell growth in OVCAR3 cells, wh

131 RNA interference (RNAi)-mediated gene knockdown is a potent approach for studying gene fu

132 to other CRISPRi systems, dCas9Sth1-mediated gene knockdown is robust when targeted far from the tran

133 a substrate for ABCA3 was performed by shRNA gene knockdown (KD) in THP-1 monocytes.

134 nd -4, as well as an additional 49 of the 62 genes, knockdown (KD) in somatic cells had minimal effec

135 period of at least 3days, leading to target gene knockdown lasting at least 10days.

136 d significantly enhanced cellular uptake and gene knockdown mediated by Tf-PEI polyplexes in human pr

137 used a lentivirus-mediated short hairpin RNA gene knockdown method to study the role of PTEN in CD34(

138 Antisense and RNAi-based gene-knockdown methods vary in efficacy between differen

139 Our results highlight zebrafish gene knockdown-mRNA rescue as an approach that can be us

140 BbCypE and DeltaBbCyp6) and the simultaneous gene knockdown mutant constructs (SiRNA30).

141 Gene knockdown occurred within a few hours of release an

142 Gene knockdown of Bad, Bid, Akt1, Akt2, PKC-mu, PKC-epsi

143 Finally, targeted gene knockdown of cx43 in adult regenerating fins recapi

144 Gene knockdown of different BBS genes in zebrafish shows

145 ebrafish larvae (4 days post-fertilization), gene knockdown of either CBS or CSE using morpholinos at

146 Gene knockdown of IR, but not IGF-IR, prevented the char

147 -6 production was significantly reduced upon gene knockdown of myeloid differentiation primary respon

148 Gene knockdown of nlz1 and/or nlz2 in zebrafish leads to

149 RNA (siRNA) technology to produce a targeted gene knockdown of NPY and dopamine-beta-hydroxylase (DBH

150 up-regulated in acute pancreatitis and that gene knockdown of PAP correlated with worsening severity

151 Gene knockdown of potential RI-interacting AKAPs express

152 Gene knockdown of PP5 abolished the estrogen-mediated su

153 Inhibition or gene knockdown of PRL-3 did not reduce ULBP2 shedding, b

154 when the c-Met receptor was deactivated, and gene knockdown of PTP1B increased c-Met activation.

155 f COPII function by oligonucleotide-mediated gene knockdown of sec31a and sec31b or brefeldin A treat

156 Gene knockdown of SLC30A2 in mammary epithelial cells re

157 Conversely, gene knockdown of STAT1 in the metastatic TM40D-MB cells

158 Using morpholino-mediated gene knockdown of TOR pathway components, we show that t

159 Using temporal gene knockdown of zebrafish samhd1, we observe hindbrain

160 ed for the expression of PTN in macrophages, gene knockdowns of these transcription factors were perf

161 in luminal tumors expressed basal epithelial genes, knockdown of either K14 or p63 was sufficient to

162 Among these GABP-associated genes, knockdown of GABPalpha expression by RNA interfer

163 Among the human homologs of candidate genes, knockdown of PPP2R1A, a gene encoding a constant

164 Consistent with early expression of these genes, knockdown of syngap1b or shank3a cause common emb

165 zebrafish development and the effects of ccp gene knockdown on cilia formation, morphology, and tubul

166 We also performed targeted gene knockdown on this model by directly infecting expla

167 filing gene function by analyzing effects of gene knockdowns on the architecture of a complex tissue

168 Inhibition of RET either by gene knockdown or by treatment with sunitinib or vandeta

169 bromide] after siRNA (small interfering RNA) gene knockdown or drug treatment.

170 eatment of both cell lines with either Flk-1 gene knockdown or Flk-1 kinase inhibitor SU1498 abrogate

171 oligonucleotides designed to mediate either gene knockdown or gene knockout, coupled with next-gener

172 can be applied for inducible and reversible gene knockdown or gene overexpression in many different

173 y developing single-step optimized inducible gene knockdown or knockout (sOPTiKD or sOPTiKO) platform

174 tightly controlled individual or multiplexed gene knockdown or knockout in hPSCs and in a wide variet

175 lia was significantly inhibited by PPARgamma gene knockdown or neutralizing anti-CD36 antibody, where

176 ere enables mosaic screens in the context of gene knockdown or overexpression by automatically genera

177 the ability to accelerate genetic studies by gene knockdown or overexpression, have led to the widesp

178 hibition of beta1 in tumor cells with stable gene knockdown or treatment with OS2966, a neutralizing

179 rs of high-throughput screens categorize how gene knockdowns or small molecules can change clock peri

180 ies reported prevalent lethality among young gene knockdowns, our phylogenomic analyses reveal that y

181 Pharmacologic and gene knockdown/overexpression approaches were used to in

182 siRNAs) is a powerful new tool for analyzing gene knockdown phenotypes in living mammalian cells.

183 I RNA-targeting CRISPR-Cas system as a novel gene knockdown platform to investigate gene function and

184 been widely exploited for sequence-specific gene knockdown, predominantly to investigate gene functi

185 Subsequently, HDAC9 gene knockdown produced dose-dependent gamma-globin gene

186 lar manipulations, including overexpression, gene knockdown, PSD-93 knock-out mice combined with bioc

187 -type calcium channel accessory beta-subunit gene knockdown reduced calcium transient amplitude.

188 In contrast, HO-1 gene knockdown reduced the survival of intramacrophage B

189 ation of histone 3 at lysine 4, whereas TLR8 gene knockdown reduced these effects.

190 owever, the efficacy of exogenous siRNAs for gene knockdown remains hampered by their susceptibility

191 Here, we developed an endogenous gene knockdown/rescue strategy that combines RNAi select

192 This localized gene knockdown resulted in behavioral changes, including

193 or by small interfering RNA (siRNA)-mediated gene knockdown resulted in c-Myc down-regulation, which,

194 Homer1 gene knockdown resulted in increased GnRH-induced FSHbet

195 In contrast, tcf3 gene knockdown results in a reduced mitotic index withou

196 We show that tcf7 gene knockdown results in dorsal patterning defects with

197 Single gene knockdowns reveal that beetle Robos have specialize

198 RNA interference-mediated gene knockdown revealed that osteoinductive BMP activiti

199 Small interfering RNA (siRNA)-mediated gene knockdown revealed that the inflammatory potential

200 Conditional gene knockdown reveals that inhibition of ROCKII promote

201 also been deployed in genome-wide, specific gene-knockdown screens.

202 Inactivation of SYK by inhibitors or gene knockdown sensitized paclitaxel cytotoxicity in vit

203 BXAP) was found to be the only gene in which gene knockdown sensitized tumor cells to paclitaxel.

204 but not 53BP1 heterozygous tumors or partial gene knockdown) sensitizes glioma cells to ionizing radi

205 Gene knockdown showed a role for SOX9 in cell migration

206 us mRNA during tetracycline-dependent target gene knockdown, significantly enhancing the experimental

207 The phenotypes of the gene knockdowns sorted into five distinct phenotypic cla

208 cultured neuronal cells, we found that SRC-1 gene knockdown specifically in the NTS significantly dim

209 over-expression strains, and a simultaneous gene knockdown strategy using tandem SiRNA.

210 Gene knockdown studies demonstrated that HDAC1 and HDAC2

211 Gene knockdown studies revealed that tumor eradication r

212 Gene knockdown studies showed that the ERAD E3s Rma1 and

213 l cell polarity across tissues, we performed gene knockdown studies to assess the roles of the relate

214 ar system can be extended toward multiplexed gene knockdown studies, as demonstrated in a two color p

215 karyotic parasite Trypanosoma brucei through gene knockdown studies.

216 opoisomerase levels as predicted by in vitro gene knockdown studies.

217 ng potent siRNAs and facilitating functional gene knockdown studies.

218 regulated VSMC contractility, based on siRNA gene knockdown studies.

219 Gene-knockdown studies in AsPC-1 and HPAF-2 cell lines c

220 ockade of YKL-40 using small-interfering RNA gene knockdown suppressed tumor angiogenesis in vitro an

221 eported a plasmid-based, tamoxifen-inducible gene knockdown system in cultured cells using a combined

222 diomyocytes and a short hairpin RNA-mediated gene knockdown system of the bag3 gene were performed.

223 tes an underappreciated shortcoming of siRNA gene knockdown technology.

224 Furthermore, Plk1 gene knockdown through Plk1-specific shRNA or its activi

225 Here we used overexpression and gene knockdown to investigate the role in embryonic myog

226 EGS-based approach merits consideration as a gene knockdown tool in archaea.

227 provides both innate immunity and efficient gene-knockdown tools in many eukaryotic species, but cur

228 covered with more GSDCs than those in YKL-40 gene knockdown tumors.

229 ries can now be readily generated to perform gene knockdowns under various conditions, increasing the

230 veral of these technologies such as targeted gene knockdown using antisense morpholinos, small molecu

231 inhibition by 3-O-methyl-sphingomyelin or by gene knockdown using antisense oligonucleotides attenuat

232 In zebrafish, gene knockdown using morpholinos revealed a genetic inte

233 ino-4,5,6,7-tetrabromo-1H-benzimidazole) and gene knockdown using RNA interference, we identified CK2

234 PTBP1 gene knockdown was achieved via PTBP1-siRNA; restoration

235 rol siRNA cargos were loaded into hydrogels, gene knockdown was only encountered for hydrogels contai

236 Morpholino-induced transient gene knockdown was performed in zebrafish embryos.

237 iogenesis demonstrated that mirtron-mediated gene knockdown was splicing-dependent, Drosha-independen

238 By targeted gene knockdown we show the significance of this, demonst

239 logical inhibitors and small interfering RNA gene knockdown, we demonstrated that concomitant activat

240 Furthermore, using gene knockdown, we discovered that this activation is in

241 horylation of guide strands correlating with gene knockdown, we employed a peptide-nucleic acid (PNA)

242 Using cell-type specific gene knockdown, we find that both neurons and glia contr

243 Using chained amiRNAs for multi-gene knockdown, we show that concatenation of miRNAs tar

244 cal inhibitors/activators and siRNA-mediated gene knockdowns, we correlated channel activity with Rap

245 YWHAE-FAM22 fusion gene knockdowns were performed with shRNAs and siRNAs ta

246 s prompted more extensive studies of MOBKL1B gene knockdowns, which included additional siRNAs and sp

247 thione levels and (18)F-FASu uptake, whereas gene knockdown with anti-xCT small interfering RNA led t

248 thione levels and (18)F-FASu uptake, whereas gene knockdown with anti-xCT small interfering RNA led t

249 Either MYD88 or TLR8 gene knockdown with relevant siRNA reduced HIV-1 ssRNA-m

250 n determining CD8(+) effector T-cell fate by gene knockdown with RNAi is challenging because naive T

251 n of CSN3 or of icIL-1Ra1 production through gene knockdown with specific small interfering RNA in A4

252 nd demonstrated the feasibility of selective gene knockdown within specific cell types.

253 lear Cre translocation and equally effective gene knockdown without cardiomyopathy is achievable with

254 enous microRNA machinery to elicit efficient gene knockdown without impeding normal cellular function

255 otransduction in vivo in morpholino-mediated gene knockdown zebrafish.