ライフサイエンスコーパス: gene knockout

1 valuated the basis of drug susceptibility by gene knockout.

2 s disclosed the targets affected by Galpha12 gene knockout.

3 the CB2R gene knockout mice are constitutive gene knockout.

4 ium stipitatum) and investigated by targeted gene knockout.

5 cted transgenic mice following prion protein gene knockout.

6 n the early coding region, ensuring faithful gene knockout.

7 -trap-based screen and comparison with yeast gene knockouts.

8 ct of 439 small-molecule challenges on 1,448 gene knockouts.

9 they are generated and model the effects of gene knockouts.

10 n limited by the embryonic lethality of many gene knockouts.

11 characterized from a series of biosynthetic gene knockouts.

12 ed robustness assessments, as in the case of gene knockouts.

13 ation of insertions and deletions typical of gene knockouts.

14 d transcriptomes generated for non-metabolic gene knockouts.

15 RISPR/Cas9 nuclease is commonly used to make gene knockouts.

16 ing the production of the first 1,751 unique gene knockouts.

17 nts that are more severe than in other cilia gene knockouts.

18 Disruption of this binding or GPR110 gene knockout abolishes while GPR110 overexpression enha

19 een skeletal muscle samples from adiponectin gene knockout, adiponectin-reconstituted, and control mi

20 e of hypoadiponectinemia in GDM, adiponectin gene knockout (Adipoq(-/-) ) and wild-type (WT) mice wer

21 However, adiponectin gene knockout (Adipoq(-/-)) attenuated maternal obesity-

22 15% were achieved with RPT and RMT, and >50% gene knockout after RET.

23 Mouse Tor1a gene knockout also specifically affects the developing n

24 Gene knockout analysis demonstrated that functional coop

25 Gene knockout analysis shows that MadB1-4 contribute to

26 ssues or primary adipocytes from adiponectin gene knockout and control mice.

27 Here we provide evidence from vGPCR gene knockout and depletion experiments that vGPCR is a

28 nstitutes a unique tool to spatially control gene knockout and greatly broadens the scope of loss-of-

29 nisms lacking facile mechanisms for targeted gene knockout and homologous recombination.

30 The combination of SIRT1 gene knockout and imatinib treatment further extends the

31 Gene knockout and knockdown methods were used to examine

32 ensive use of multiple CRISPR/Cas9-generated gene knockout and knockin cell lines to investigate the

33 eplicating plasmids and suicide plasmids for gene knockout and knockin.

34 n this study, we used mouse models of Kiss1r gene knockout and mouse mammary tumor virus-polyoma viru

35 didates to be tested in strategies involving gene knockout and overexpression in plants.

36 Gene knockout and RNAi in individual neurons reveal that

37 Furthermore, gene knockout and selective pharmacological inhibition o

38 ential functions, but studies using targeted gene knockout and siRNA-mediated transcript knockdown to

39 tered by nitrogen-limitation and proteasomal gene knockout and spanned various functions including co

40 utcome was the sequential CpFTSY and ZEP two-gene knockout and the generation of a strain constitutiv

41 Abeta degradation in vivo through the use of gene knockout and transgenic mice.

42 Because of the development of gene knockout and transgenic technologies, small animals

43 R-2-deficient mice were generated by SLC19A3 gene knockout and used to examine intestinal uptake of t

44 Six-week-old CSE gene knockout and wild-type mice were fed with either a

45 ly validated in Arabidopsis thaliana through gene knockout and/or overexpression studies.

46 binding site, (ii) generating translational gene knockouts and (iii) introducing other coding or non

47 Using BBS gene knockouts and a mouse infection model, we show the

48 observed no significant relationship between gene knockouts and clinical consultation or prescription

49 t ergot alkaloid mutants derived by previous gene knockouts and differing in ergot alkaloid profiles.

50 been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells.

51 describes the use of this technique to make gene knockouts and knock-ins, as well as single-nucleoti

52 ptimized to create either single or multiple gene knockouts and large chromosomal deletions.

53 Using data from five single gene knockouts and the wild type strain, we decrease the

54 otein interaction, subcellular localization, gene knockout, and bioinformatics analyses.

55 f pendrin under conditions not confounded by gene knockout, and to test the potential utility of pend

56 dvances in cell labeling, microscopy, single-gene knockouts, and genomics, as well as the results of

57 Single gene knockouts appear to have minimal effect on morpholo

58 These results suggest that the gene knockout approach may be able reduce antigenicity i

59 dopamine (DA) system, we used a conditional gene knockout approach to selectively delete GRK2 in DA

60 Using a conditional gene knockout approach, the alpha isoform of p38 mitogen

61 Using a genome-wide gene knockout approach, we identified Keap1, the SWI/SNF

62 Using a tissue-specific gene knockout approach, we inactivated Shp2 expression i

63 Using a gene knockout approach, we report that Cdk2 and Cdk4 are

64 Although this gene 'knockout' approach is often informative, in many c

65 to discrepancies between pharmacological and gene knockout approaches and opposite influences on coca

66 study, using T cell receptor transgenic and gene knockout approaches, we provided definitive evidenc

67 which precludes investigation using classic gene knockout approaches.

68 hat rare autosomal and X chromosome complete gene knockouts are important inherited risk factors for

69 son- and zinc finger nuclease (ZFN)-mediated gene knockout as well as the establishment and culturing

70 ties on various substrates and the effect of gene knockouts at the genome scale.

71 y, when bred onto a recombination activating gene knockout background, POT mice developed a fulminant

72 ructural components of the SPN, and alveolin gene knockout causes morphological abnormalities that co

73 plasma glucose and insulin secretion of CCK gene knockout (CCK-KO) mice and their wild-type controls

74 deletion of expression using the conditional gene knockout (cKO) approach.

75 haracterized Cre-loxP system for conditional gene knockout (CKO) in mice.

76 genes whose roles were probed via individual gene knockouts, construction of over-expression strains,

77 designed to mediate either gene knockdown or gene knockout, coupled with next-generation sequencing.

78 Cardiomyocyte-restricted Csn8 gene knockout (CR-Csn8KO) in mice was achieved using a C

79 Gene knockouts, deletions and point mutations are readil

80 ting THIK-1 function pharmacologically or by gene knockout depolarizes microglia, which decreases mic

81 f-2alpha through various combinations of Phd gene knockouts did not further improve hepatic metabolis

82 nked hydrogenase enzymatic activity, but hyd gene knockouts did not influence the hydrogen yield of c

83 t for most miRNA genes of C. elegans, single-gene knockouts did not result in detectable mutant pheno

84 C lines, as well as stage-specific inducible gene knockout during hPSC differentiation.

85 cytosine or guanine significantly increases gene knockout efficiency in CRISPR-Cas9-based genome edi

86 We have also compared pairwise gene knockout essentiality predictions for 10 of these m

87 Single-gene knockout experiments can fail to reveal function in

88 Gene knockout experiments in mice have suggested a hiera

89 ative genomics approaches and the results of gene knockout experiments were used to extrapolate the m

90 complementation tests and CRISPR/Cas9-based gene knockout experiments.

91 Gene-knockout experiments on single-cell organisms have

92 We also conclude that whole-gene "knockouts" fail to reveal how important gene produ

93 al infection using small interfering RNA and gene knockout fibroblasts, implicating cytosolic DNA as

94 stmitotic neurons using in vivo and in vitro gene knockout for the mitochondrial division protein Drp

95 Optimization procedures to identify gene knockouts for targeted biochemical overproduction h

96 entional "selectivity filter." AQP2-specific gene knockout generated MPXR trypanosomes but did not af

97 alpha-Galactosidase A gene knockout (Gla KO) mice have no alpha-galactosidase

98 alpha1,3-Galactosyltransferase gene knockout (GTKO) pigs reduced the significance of an

99 hy (TM) after alpha1,3-galactosyltransferase gene-knockout (GTKO) pig organ transplantation in primat

100 f livers from alpha1,3-galactosyltransferase gene-knockout (GTKO, n=1) or GTKO pigs transgenic for CD

101 ineered pigs (alpha1,3-galactosyltransferase gene-knockout [GTKO] pigs and pigs expressing a human co

102 Over the last approximately 30 y, gene knockouts have revealed that CaV1.1/RyR1 coupling r

103 nt one-step generation of double- and triple-gene knockout hPSC lines, as well as stage-specific indu

104 Magnetization-based screening of gene knockouts identified Tco89p, a component of TORC1 (

105 potent stem cell (hPSC) lines with inducible gene knockout (iKO) remains challenging.

106 Rdx gene knockout impairs reversal learning and short-term m

107 ave emerged as potent new tools for targeted gene knockout in bacteria, yeast, fruit fly, zebrafish a

108 Despite efficient gene knockout in embryonic day 14.5 (E14.5) dermal conde

109 In this study, we show that Ehd1 gene knockout in mice on a predominantly B6 background i

110 aracterized Cre-loxP systems for conditional gene knockout in mice, and studied the effects of these

111 o 1,500-fold in cell lines and to facilitate gene knockout in mouse zygotes without sacrificing on-ta

112 The development of CRISPR/Cas9-mediated gene knockout in two ant species opens a new window into

113 e editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

114 Using multiple gene knockouts in Arabidopsis thaliana, we investigated

115 ugh the dopamine D2 receptor with the use of gene knockouts in Caenorhabditis elegans or RNA interfer

116 nd scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pl

117 d the entire KEIO collection of 3,985 single-gene knockouts in Escherichia coli for increased suscept

118 ire Keio collection of close to 4,000 single-gene knockouts in Escherichia coli for increased suscept

119 ology has enabled the rapid establishment of gene knockouts in many cell types and even whole organis

120 Here, using gene knockouts in mice, we evaluated genetic interaction

121 Phenotypic variation among gene knockouts in S. cerevisiae fits a double Pareto-log

122 Here, we generated TERC (telomerase RNA) gene knockouts in telomerase positive cell lines that re

123 eases (ZFNs) in embryos was used to generate gene knockouts in the rat and the mouse by introducing n

124 ed cell lines that carried single and double gene knockouts in the same genetic background.

125 plementation of Escherichia coli mutants and gene knockouts in V. cholerae N16961.

126 Similarly, B6 mice with different gene knockouts including TLR2, TLR4, MyD88, and Toll-int

127 ted the effects of different combinations of gene knockout, including arsenate reductase (HAC1), gamm

128 Gene knockout indicates that TbECT is essential for grow

129 . elegans, and demonstrated that conditional gene knockout is feasible in C. elegans.

130 y of the Htt gene in CNS development through gene knockout is problematic as the knockout leads to em

131 ection of all viable Escherichia coli single-gene knockouts is facilitating a systematic investigatio

132 ced RAP1 activation in WT and integrin beta3 gene knockout (Itgb3-/-) OCs, but its effects were subst

133 RISPR/Cas9 technology for genome editing and gene knockout, its performance still relies on well-desi

134 not occur in alpha1,3-galactosyltransferase gene-knockout kidney xenografts.

135 his study, we show that PARP-1 inhibition by gene knockout, knockdown, or pharmacologic blockade prev

136 SPR/Cas9 system for generating site-specific gene knockout/knockin models, including a knockout pig m

137 We therefore investigated whether Tric-a gene knockout (KO) alters the single-channel function of

138 Fkbp5 gene knockout (KO) and wild-type (WT) mice were assessed

139 se LARP4 accumulated to higher levels in TTP gene knockout (KO) cells than in control cells.

140 ted MCF7 breast cancer cell line-derived DPH gene knockout (ko) cells to assess the impact of complet

141 Mice with CD28 or CD80/CD86 gene knockout (KO) displayed an infection course similar

142 Gene knockout (KO) does not always result in phenotypic

143 LEN based methods for accomplishing complete gene knockout (KO) in AML cells.

144 yonic fibroblasts (MEFs) derived from target gene knockout (KO) mice can be stable isotope-tagged and

145 We generated TL1A gene knockout (KO) mice to assess its role in collagen-i

146 pe (WT) mice were compared with those in CSE gene knockout (KO) mice.

147 efficiencies on generating Factor VII (FVII) gene-knockout (KO) mice.

148 diseases, we have not yet explored how such gene knockouts (KOs) could influence complex human pheno

149 B. bassiana targeted gene knockouts lacking Bbcyp52x1 displayed reduced virul

150 somal acid lipase (hLAL) expression into LAL gene knockout (lal(-/-)) mice.

151 In LAL gene-knockout (lal(-/-)) mice, blockage of cholesteryl e

152 suggest that an increase in EETs due to sEH-gene knockout leads to an increase in the size of islets

153 CRISPR/Cas9-based gene knockout libraries have emerged as a powerful tool

154 We fit a large published data set of single-gene knockout lines in Saccharomyces cerevisiae to seven

155 otypic variation in a large series of single-gene knockout lines than other skewed or symmetrical dis

156 a complex, the phenotypic consequences of a gene knockout may manifest as a combination of several d

157 Treatment of multidrug resistance 2 gene knockout (Mdr2(-/-) ) mice with A-1331852 resulted

158 s using Polk(-/-) Polh(-/-) Poli(-/-) triple-gene knockout MEFs unexpectedly revealed that another po

159 m represents a highly effective and scalable gene knockout method in zebrafish and has the potential

160 ic effects, we analyzed these factors in Ada gene knockout mice (Ada(-/-)).

161 bolished in pancreatic beta cells from Tpcn2 gene knockout mice [2].

162 Using gene knockout mice and in vivo cellular depletion method

163 d lipolysis was observed in both adiponectin gene knockout mice and primary adipocytes from these mic

164 ll as in plasma samples from six cholestatic gene knockout mice and six age- and gender-matched wild-

165 almitoyltransferase (SPT) subunit 2 (Sptlc2) gene knockout mice and sphingomyelin synthase 2 (Sms2) g

166 METHODS AND We used H- and K-Ras gene knockout mice and subjected them to pressure overlo

167 ecific mechanisms are unclear since the CB2R gene knockout mice are constitutive gene knockout.

168 ntribution to its etiology, including single-gene knockout mice associated with diaphragmatic defects

169 However, studies in connexin-gene knockout mice demonstrated significant CV slowing o

170 -1(-/-)) and heterozygous (Flt-1(+/-)) Flt-1 gene knockout mice display increased endothelial cell pr

171 ing to echocardiography, we found that Efnb3 gene knockout mice showed enhanced constriction in the c

172 Accordingly, cultured T-cells from Spry1 gene knockout mice showed increased proliferation in res

173 nimal model for DMD, were crossed with Flt-1 gene knockout mice to create a model with increased vasc

174 he central corneal epithelium of C57BL/6 and gene knockout mice was abraded, and 1 x 10(7) S. marcesc

175 ood pressure (shared by some but not all BBS gene knockout mice).

176 We generated Necl4 gene knockout mice, but found that disruption of Necl-4

177 ing into question negative results in single-gene knockout mice.

178 ere markedly reduced in TNF receptor 1 and 2 gene knockout mice.

179 s can predict the lethal phenotype of single-gene knockout mice.

180 where possible, testing of islet tissue from gene knockout mice.

181 out mice and sphingomyelin synthase 2 (Sms2) gene knockout mice.

182 rkedly reduced in tamoxifen-inducible Nkx2-5 gene knockout mice.

183 r mice, an adoptive cell transfer model, and gene knockout mice.

184 d a streptozotocin-induced DKD model in FHL2 gene-knockout mice to determine the possible role of FHL

185 ransgenic or alpha-1,3-galactosyltransferase gene knockout miniature swine.

186 Using MMP-9 gene knockout (MMP-KO) mice, we investigated the effect

187 esent what we believe to be the first single gene-knockout model for HNSCC, in which both HNSCC forma

188 Using different murine lymphocyte gene knockout models, we show that the enhanced sensitiv

189 death or are lethal in the orthologous mouse gene knockout models.

190 strategy to generate the whole body dematin gene knockout mouse model (FLKO).

191 We used a pan-DC, CD11c-specific cre-lox gene knockout mouse model to assess the role of KLF2 in

192 After a decade of extensive work on gene knockout mouse models of cell-cycle regulators, the

193 Using a gene-knockout mouse model, we demonstrate that the admin

194 h in ATL, xylose metabolism was targeted for gene knockout mutagenesis.

195 Expressing the two genes in trans in the two-gene knockout mutant complemented full virulence.

196 Through a series of markerless gene knockout mutants and complementation tests, specifi

197 rases were determined via SMRT sequencing of gene knockout mutants.

198 locity particle bombardment to engineer 1201 gene knockout mutants.

199 evisiae and that several single and multiple gene-knockout mutants are computationally predicted to i

200 Here, we report the generation of gene knockout NRG mice by combining in vitro fertilizati

201 inding and opioid analgesic assays; however, gene knockout of JNK 1 selectively blocked kappa-recepto

202 IL-24 lethality, whereas RNA interference or gene knockout of Mcl-1 markedly sensitized transformed c

203 Indeed, gene knockout of Polbeta caused mitochondrial dysfunctio

204 and in vivo characterization of the targeted gene knockout of this novel NF-kappaB inhibitor in CPXV.

205 hy MS methods, we have screened 1,755 single gene knockouts of Escherichia coli and have identified t

206 s in controlling VV infections, mouse single-gene knockouts of RNase L and PKR and double-knockout mi

207 Gene knockouts of the Bacillus C-methyltransferase and t

208 Targeted gene-knockout of Bbmsn2 resulted in reduced growth and i

209 Gene knockouts often yield limited insights into functio

210 ter striatal ICH, the effect of IRP1 or IRP2 gene knockout on ferritin expression and injury was quan

211 with conflicting data on the effects of Prnp gene knockout on various parameters of T-cell immunity.

212 macrophages showed that inhibiting ACAT1 by gene knockout or by pharmacological inhibition caused a

213 Mice with global IL-1 receptor gene knockout or central IL-6 receptor knockdown showed

214 ations, has been limited by lack of a robust gene knockout or knock-down technology.

215 Further, either Ido1 gene knockout or pharmacological inhibition of hippocamp

216 asma cells were inherently sensitive to Rab7 gene knockout or Rab7 activity inhibition in class switc

217 achieved by modifying the genotype (such as gene knockouts or knockins) under the scope of genome/pr

218 Except for DeltaBbCypA, targeted gene knockouts or overexpression of any cyclophilin resu

219 rodent models of arrhythmia caused by clock gene knockouts or surgical ablation of the suprachiasmat

220 PfHsp110c gene-knockout parasites were not viable and conditional

221 rved cell-autonomous circadian phenotypes of gene knockouts, particularly retention of rhythmicity an

222 ole of SIGLEC1 in PRRSV infection, a SIGLEC1 gene knockout pig was created by removing part of exon 1

223 After alpha1,3-galactosyltransferase gene-knockout pig artery patch xenotransplantation, reci

224 dy binding to alpha1,3-galactosyltransferase gene-knockout pig cells was less than to wild-type cells

225 ic changes in alpha1,3-galactosyltransferase gene-knockout pig kidneys transplanted into baboons that

226 ografts from alpha 1-3 galactosyltransferase gene knockout pigs, which express human complement regul

227 wild-type and alpha1,3-galactosyltransferase gene-knockout pigs) and anti-Gal IgM and IgG levels (by

228 currently the fastest algorithm for finding gene knockouts predicted by FBA to increase production o

229 Using gene knockout, protein depletion, and overexpression stu

230 ribe here a detailed protocol for generating gene knockout rats by homologous recombination in embryo

231 Here we report the generation of gene knockout rats using the ES-cell-based gene targetin

232 rotocol to generate Tp53 (also known as p53) gene knockout rats.

233 line via ES-cell rat chimaeras to create p53 gene knockout rats.

234 l antibody or tumor necrosis factor receptor gene knockout, reduced inflammation and retinal ganglion

235 survivals of alpha1,3-galactosyltransferase gene knockout renal xenografts in this laboratory correl

236 rs or specific small interfering RNA or JAK3 gene knockout resulted in an increase in TLR-mediated pr

237 lpha, beta, gamma, delta), only pPLAIIIdelta gene knockout results in a decrease in seed oil content,

238 Pcgf3/5 gene knockout results in female-specific embryo lethalit

239 RPE65 gene knockout (Rpe65(-)/(-)) mice showed abolished isome

240 of the CRISPR-Cas9 system to pooled library gene knockout screens in mammalian cells represents a ma

241 , a supervised learning method for analyzing gene knockout screens.

242 own, and mouse cells lacking the protein via gene knockout showed increased sensitivity to oxidative

243 Somatic gene knockouts showed both genes are required for effici

244 -based metabolic modeling and computer-aided gene knockout simulations to identify five genes (ALT2,

245 e report the successful generation of global gene-knockout, single-amino-acid-substituted, as well as

246 Disrupting TPC function by gene knockout, small interfering RNAs, or small-molecule

247 A REP27 gene knockout strain of Chlamydomonas, termed the rep27

248 Unlike wild-type, most gene knockout strains were found to have one additional

249 The optimization problem to derive gene knockout strategies recently has been formulated as

250 Antibody neutralization and gene knockout strategies were used to show that the pres

251 Gene knockout strategies, RNAi and rescue experiments ar

252 By utilizing gene knockout strategies, we created S. aureus N315 muta

253 e uterine epithelium using a tissue-specific gene knockout strategy, in which floxed Mig-6 (Mig-6(f/f

254 clusions were unattainable by using standard gene knockout studies alone.

255 SPR/Cas9 is a powerful gene editing tool for gene knockout studies and functional genomic screens.

256 intersected with the findings of three siRNA gene knockout studies and identified potential drug targ

257 tional expression in heterologous system and gene knockout studies has shown their functions as taste

258 Gene knockout studies have highlighted this enzyme as a

259 However, gene knockout studies have reached disparate conclusions

260 Gene knockout studies have revealed that TRAF2 inhibits

261 Whereas gene knockout studies in mice have shown that both the O

262 Gene knockout studies in the bloodstream form of the par

263 In sum, our results show that single-gene knockout studies of a progenitor virus can have lit

264 Evidence obtained from gene knockout studies supports the role of Toll-like rec

265 tions encompassing the consequences of mouse gene knockout studies, is a principal component of mouse

266 Our results from gene knockout studies, LSC tracing and transplantation m

267 ously identified as virulence genes in whole-gene knockout studies.

268 eleterious alternative to wild-type Cas9 for gene-knockout studies.

269 Flow cytometry showed that RBC from 2-gene knockout swine exhibited less human antibody bindin

270 pCMV into our alpha1,3-galactosyltransferase gene knockout swine herd.

271 Here we have used a conditional gene knockout system to delete PKC lambda/iota selective

272 By using a gene knockout system to inactivate the toxin genes perma

273 only mimic the in vivo setting, and current gene-knockout technologies do not allow the simultaneous

274 y gland development, using Cre-loxP-mediated gene knockout technology.

275 Here we show via gene knockout that whereas DraRnl is inessential for gro

276 and describe naturally occurring homozygous gene knockouts that suggest the dispensability of a vari

277 previously published reports of conditional gene knockouts that used the Trp1-Cre mouse, suggesting

278 t optimization to identify single and double gene-knockouts that resulted in increased polyketide pro

279 Six weeks after Pkd1 global gene knockout, the mice were treated with Agt-ASO (66 mg

280 d as an effective tool for sequence-specific gene knockout through non-homologous end joining (NHEJ),

281 CRISPR/Cas9 is used extensively to engineer gene knockouts through NHEJ, editing by HDR remains inef

282 When TLR4 gene knockout (TLR4(-/-)) and TLR4-competent (TLR4(+/+))

283 d megalin/cubilin-deficient mice produced by gene knockout to determine whether receptor-mediated end

284 on microscopy, single-molecule tracking, and gene knockouts to examine the dynamics of individual Tcp

285 , from high-throughput phenotyping of single-gene knockouts under hundreds of annotated conditions, f

286 ncing (RNA-seq) and from RNA interference to gene knockouts using Clustered Regularly Interspaced Sho

287 to produce RBCs that allow the generation of gene knockouts via CRISPR/Cas9 using the immortal JK-1 e

288 SH gene knockout virus (RSV DeltaSH) is partially attenuate

289 loid-specific cre-expressing mice, effective gene knockout was achieved in various myeloid cells.

290 ut mice were generated and the effect of the gene knockout was determined, including COX activity, ti

291 maging, force measurements, pharmacology and gene knockout, we show that dynamic assembly of filament

292 e-specific neuron silencing, and conditional gene knockout, we show that these molecularly defined 5H

293 bining parabiosis, single-cell analyses, and gene knockouts, we uncover a multi-organ web of tissue-r

294 nts' clinical phenotypes to laboratory mouse gene knockouts, we were able to strongly implicate anoma

295 Isotope feeding and gene knockout were employed in the engineered S. lividan

296 As rhoph2 and rhoph3 gene knockouts were not viable in the human P. falciparu

297 Moreover, elimination of NeuroD1 by gene knockout, while it does not disrupt initial OSN dif

298 or alpha2 could be separately abolished via gene knockout with little to no impact on growth, condit

299 ous approach also enabled a screen combining gene knockouts with transcriptional activation, which re

300 Examining complete gene knockouts within a viable organism can inform on ge