ライフサイエンスコーパス: gene of interest

1 limit the scope of orthology search for each gene of interest.

2 ull-length 3'-UTR reporter construct for the gene of interest.

3 s) regulate the transcriptional process of a gene of interest.

4 tation in the Crb1 gene rather than with the gene of interest.

5 to the cloning and transfer of just a single gene of interest.

6 The approach can be easily applied to any gene of interest.

7 laying the iron-mimic peptide and carrying a gene of interest.

8 tered to replace one of the viral genes by a gene of interest.

9 ciated to their favorite cancer, drug and/or gene of interest.

10 tion of mutations in almost any nonessential gene of interest.

11 ying potential zinc finger target sites in a gene of interest.

12 3 was used to extract optimal primers from a gene of interest.

13 form the proper architecture to activate the gene of interest.

14 ion on shRNA constructs that can silence the gene of interest.

15 difficult to assess regulation of any given gene of interest.

16 ose siRNA sequences that are specific to the gene of interest.

17 a green fluorescence reporter plasmid for a gene of interest.

18 consider a single factor, the presence of a gene of interest.

19 ent is integrated upstream of the ATG of the gene of interest.

20 ficial single nucleotide polymorphism in the gene of interest.

21 ntial expression and survival analysis for a gene of interest.

22 on both to patient outcome and to a specific gene of interest.

23 iable-a repressor protein, which acts on the gene of interest.

24 nables assessing the regulation of any human gene of interest.

25 to a relatively short expression time of the gene of interest.

26 endogenous expression and regulation of the gene of interest.

27 targets thereby inactivating a co-expressed gene of interest.

28 e RNAs that target respectively HPRT and the gene of interest.

29 nitude of different noise sources in a given gene of interest.

30 r the design of highly active sgRNAs for any gene of interest.

31 plasmids, are extensively used to express a gene of interest.

32 specific cellular processes together with a gene of interest.

33 alian cells, to regulate the expression of a gene of interest.

34 used to examine a particular protein coding gene of interest.

35 sgRNAs for transcriptional repression of any gene of interest.

36 ng to assay for successful expression of the gene of interest.

37 cy and size of transcription bursts from the gene of interest.

38 serted into a gene-loading site along with a gene of interest.

39 dvantages that could be exploited to silence genes of interest.

40 on of fully virulent ECTV expressing foreign genes of interest.

41 miRNAs or tasiRNAs that potentially regulate genes of interest.

42 tion of target genes by suppressing specific genes of interest.

43 , and to contribute annotations for specific genes of interest.

44 s in the absence of genetic variation in the genes of interest.

45 disease phenotypes unrelated to the gene or genes of interest.

46 ion, users need to provide the GI IDs of the genes of interest.

47 ssary distance between promoter elements and genes of interest.

48 was performed, followed by validation of the genes of interest.

49 entifying plants carrying point mutations in genes of interest.

50 of knocking down the expression of candidate genes of interest.

51 length polymorphism for polymorphisms in 10 genes of interest.

52 ter for imprecise excision screens to delete genes of interest.

53 ally change the search criteria to fit their genes of interest.

54 dation tags are fused onto the 3' end of the genes of interest.

55 drug-response and clinical implications for genes of interest.

56 this library, we determined expression of 27 genes of interest.

57 tissue material) sufficient to test > or =25 genes of interest.

58 sponsive elements) associated with potential genes of interest.

59 and analysis resulting in annotated lists of genes of interest.

60 ripts, including metabolic and adipocytokine genes of interest.

61 ively fast technique to assess expression of genes of interest.

62 environmental bacteria carrying two or more genes of interest.

63 ify suitable TFO target sites within or near genes of interest.

64 ow possible to inactivate or delete selected genes of interest.

65 tified from transcription control regions of genes of interest.

66 lusters of BAC contigs in regions containing genes of interest.

67 can be used to enrich in vitro for traps in genes of interest.

68 to submit FASTA files for their genomes and genes of interest.

69 ion of the gene expression changes for seven genes of interest.

70 AC clones that can be screened in silico for genes of interest.

71 ily available for further experimentation on genes of interest.

72 allelic series of induced point mutations in genes of interest.

73 data, and then permits the user to retrieve genes of interest.

74 ers to retrieve primer information for their genes of interest.

75 ce with mutations or deletions in endogenous genes of interest.

76 that affect expression of a wide variety of genes of interest.

77 transcriptional or translational fusions to genes of interest.

78 the transposon insertion tolerance of normal genes of interest.

79 ifficulties in obtaining mutants in specific genes of interest.

80 the CAPS and dCAPS markers derived from the genes of interest.

81 approach to target and locally assemble the genes of interest.

82 ibility to study any custom miRNAs or target genes of interest.

83 of allowing researchers to focus on specific genes of interest.

84 morphisms in and around positional candidate genes of interest.

85 ic system to simultaneously express multiple genes of interest.

86 rogramming of the system to silence specific genes of interest.

87 n and validation, data triaging, and example genes of interest.

88 e and model organisms to selectively silence genes of interest.

89 ul for transient expression of various other genes-of-interest.

90 ws: (1) querying the regulatory network of a gene of interest, (2) prediction and interspecies transf

91 For any gene of interest, a probe that targets protein levels mu

92 the RSV promoter (to drive expression of the gene-of-interest) along with a multiple cloning site (MC

93 that advancement starts in this paper with a gene of interest and a characteristic function of that g

94 tion of TFBSs occurring in the promoter of a gene of interest and also the common factors predicted t

95 of the need to re-calculate trees for every gene of interest and each time a new data set is analyze

96 generates a bicistronic message encoding the gene of interest and GFP, thus enabling identification o

97 tissue-related functions for any Arabidopsis gene of interest and predictions concerning the relatedn

98 b) clone of genomic material surrounding the gene of interest and use of this clone to establish a re

99 RNAi is used to systematically down-regulate genes of interest and analyze their roles in a biologica

100 Genes of interest and cell lineage markers were examined

101 ser-friendly interface for quickly searching genes of interest and downloading genome-wide results.

102 on of mRNA levels for functional analysis of genes of interest and enable efficient exploration of ge

103 rget specific areas in the genome, including genes of interest and linkage regions, but this limits t

104 This analysis confirmed translation of the genes of interest and showed that NKCC1, Na+,K+-ATPase a

105 ed by screening for induced mutations in two genes of interest and the induced mutations were validat

106 oolset allows for consistent transduction of genes of interest and will be a powerful molecular tool

107 le as 150 cells (as few as 2 transcripts per gene of interest) and perform mass-spectrometric analyse

108 rticular genomic region or associated with a gene of interest, and assign enhancers and their target

109 r imaging systems do not directly detect the gene of interest, and most do not exploit radiopharmaceu

110 en type of epigenetic remodeling to a single gene of interest, and to probe the functional relevance

111 tiple genes, (ii) altering specific bases in genes of interest, and (iii) engineering genes to encode

112 ned for a reference gene and one of a set of genes of interest, and builds a model VirtualEmbryo.

113 levels of expression similar to those of the genes of interest, and its expression was not modified b

114 A number of genes of interest are located within the frequently aber

115 Second, because the genes of interest are not amplified by PCR, there are pr

116 cell signaling and the targeting of specific genes of interest are providing key insights into neurob

117 First, genes of interest are robustly identified in a pooled ge

118 ing a specific condition to a reference, the genes of interest are those which are differentially exp

119 tists looking for functional clues for their genes of interest as well as for curators looking for in

120 ieved by repeating orthology searches on one gene of interest at a time, thereby generating a reduced

121 The candidate interval contains several genes of interest because of their potential role in eit

122 isogenic sets of strains with mutations in a gene of interest but not in other genes by repeated use

123 nthetic genes encode the same product as the gene of interest, but the synthetic nucleotide sequence

124 an epitope tag or a fluorescent protein to a gene of interest by CRISPR-Cas9-mediated homology-direct

125 ase is first targeted to the vicinity of the gene of interest by homologous recombination.

126 ene to be expressed in the same pattern as a gene of interest by placing the Gal4 transcription facto

127 sed to accommodate, at the given site, other genes of interest by "Recombinase-Mediated Twin Site Tar

128 ays, and results were confirmed for specific genes of interest by quantitative PCR.

129 tens of thousands of base pairs flanking the gene of interest can be included in the construct.

130 ty in preparation of the constructs, since a gene of interest can be inserted into a binary vector al

131 le methods can be used in this step when the genes of interest can be divided into groups such as up-

132 minimally deleted regions in which relevant genes of interest can be found.

133 Predicted insertion mutations in genes of interest can be identified using Arabidopsis Ge

134 he URA3 gene in strains complemented for the gene of interest compared with mutant strains.

135 Choice of expression signals for the gene of interest, complications caused by the presence o

136 For the genes of interest, confirmation of gene expression was d

137 inexpensive small-scale arrays representing genes of interest could be used for many applications, i

138 it may be possible to monitor transfer of a gene of interest directly and noninvasively.

139 is easily achieved at the genomic locus of a gene of interest due to the high efficiency of homologou

140 e who do - is how to inhibit the action of a gene of interest during development so as to learn about

141 ryptophan synthase beta subunit) and another gene of interest (encoding either Ku80, an RNA-binding p

142 etic fusion of the destabilizing domain to a gene of interest ensures specificity, and the attendant

143 f cell states, but the question "Where is my gene of interest expressed?" remains one of the most dif

144 hiC31 and Bxb1 integrases was used to insert genes of interest flanked by phiC31 and Bxb1 attB sites

145 works well in creating mutations in specific genes of interest followed by complementation remains pr

146 enzae genome provide the means to target any gene of interest for mutagenesis and temperature-sensiti

147 ementary techniques identified C21orf91 as a gene of interest for susceptibility to HSL.

148 he context of self-renewal and to prioritize genes of interest for experimental validation.

149 h the bacterial secretion system to activate genes of interest for functional analysis in plants.

150 e profiling can be translated to identifying genes of interest for functional tests in the future and

151 Genes of interest for further functional analysis were s

152 sis, and Gene Carts that allow users to save genes of interest for further study while they browse.

153 Recombinant rabies viruses can encode genes of interest for labeling cells, controlling gene e

154 ector that allows a selective isolation of a gene of interest from genomic DNA in yeast followed by i

155 trix which organizes a dataset of over 2,000 genes of interest from a genome wide scan of transcripti

156 arison of protein domain organization within genes of interest from metagenomes and complete microbia

157 approaches including specific knockdowns of genes of interest from primary CD34(+) hematopoietic ste

158 the challenge for biologists is to identify genes of interest from the thousands of genetic expressi

159 e-targeted mutagenesis selects insertions in genes of interest from this library by using the PCR.

160 ng ES cell clones with mutations in specific genes of interest from this library.

161 The human gene of interest, GJB1, which is mutated in X-linked Cha

162 emid (PM) library expressing variants of the gene of interest (GOI) and preparation of the Escherichi

163 ndent on the formulation, translation of the gene of interest (GOI) inserted into the RepRNA (lucifer

164 Retroviral (RV) expression of genes of interest (GOIs) is an invaluable tool and has f

165 ing) to insert fluorescent protein tags into genes of interest harbored by transformation-competent b

166 knocking out, and introducing mutations into genes of interest has provided important insights into t

167 archers use the PSAT web interface to find a gene of interest in a reference genome and efficiently r

168 fers the possibility to knock out almost any gene of interest in an affordable and simple manner.

169 the absolute number of mRNA molecules from a gene of interest in individual, chemically fixed Escheri

170 confounded interpretation of the role of the gene of interest in lethality.

171 aRT to image the expression of any arbitrary gene of interest in living subjects.

172 PPP4R3A should be further investigated as a gene of interest in neurodegenerative diseases and as a

173 lly PCR-amplifying 150-350 bp fragments of a gene of interest in situ) and specificity (derived from

174 transfer, there is ectopic expression of the gene of interest in the plant cells.

175 to study the dynamic molecular effects of a gene of interest in the pluripotency network.

176 getable, allows high-level expression of any gene of interest in the synaptically coupled neurons, an

177 idual cells, expressing or carrying specific genes of interest in a latent form in a tissue section u

178 as for the selective knock-down of specific genes of interest in a tissue-selective manner.

179 within months) addressing the function(s) of genes of interest in a wide variety of specific cell typ

180 re can be used to evaluate other therapeutic genes of interest in animal models before future clinica

181 accurate identification of organisms or even genes of interest in complex environmental samples (air,

182 c biochip capable of accurately quantitating genes of interest in DNA samples.

183 he poplar genome sequence and identify novel genes of interest in forest biology.

184 Two genes of interest in H. akashiwo, not previously reporte

185 tile and noninvasive method of investigating genes of interest in mouse mammary epithelial cells.

186 to determine the expression profile of their genes of interest in multiple clinical data sets and sev

187 to specifically repress the transcription of genes of interest in plants.

188 Genes of interest in psoriasis tissue that did not retur

189 xplore the functional relationships of their genes of interest in specific mouse tissue contexts.

190 ling and provides a means of rapidly editing genes of interest in the heart.

191 s available for conditionally overexpressing genes of interest in the MK/platelet lineage in vivo.

192 tosidase gene, as a cellular reporter, and a gene of interest (in this case, the mitotic regulator Au

193 The genes of interest include known and novel genes encoding

194 Genes of interest include those involved in the pharmaco

195 Genes of interest included a 16S rRNA gene selective for

196 Single genes of interest included I-kappa-B kinase-alpha (CHUK)

197 tifying expression-independent properties of genes of interest including upstream transcriptional reg

198 ough isolation of full-length cDNAs for five genes of interest, including a new class of vascular-exp

199 h the BDP, the evolutionary half-time of the gene of interest increases 4-10 times, depending on the

200 ogical samples increases or as the number of genes of interest increases, respectively, and these fac

201 A method is presented to assemble a gene of interest into a linear DNA template with all the

202 he procedure involves, first, cloning of the genes of interest into an appropriate mini-Tn7 vector; s

203 he procedure involves, first, cloning of the genes of interest into an appropriate mini-Tn7 vector; s

204 he procedure involves, first, cloning of the genes of interest into an appropriate mini-Tn7 vector; s

205 viruses have been widely employed to deliver genes of interest into pancreatic islets.

206 ration of VIGS vectors carrying fragments of genes of interest into seedlings at the two- to three-le

207 ivo, and the ability to efficiently transfer genes of interest into such cells would create a number

208 Particular genes of interest involved in embryogenesis (abscisic ac

209 iable cost-effective approach, providing the gene of interest is adequately captured.

210 A gene of interest is disrupted in adult floxed mice by a

211 s particularly useful in instances where the gene of interest is essential and a knockout mouse is no

212 ry because loss (or gain) of one copy of the gene of interest is insufficient to recapitulate the dis

213 n a single homologous integration event, the gene of interest is placed under control of the thiamine

214 cassette is uniquely arranged such that the gene of interest is split and only reconstituted in the

215 hen a strain carrying a null mutation in the gene of interest is transduced with a packaged cosmid ca

216 In our method, PCR-amplified sequence of a gene of interest is used as a template for the synthesis

217 We demonstrate that when the number of genes of interest is inconveniently large, identifying a

218 onucleotide array data, especially to select genes of interest, is a highly challenging task because

219 approach employs deletion of a nonessential gene of interest (knockout), or multi-step recombineerin

220 A sequences from both sides of a transgenic "gene of interest," leaving only the desired gene and sho

221 the study of the inner ear is that loss of a gene of interest may cause embryonic lethality before th

222 However, it is possible that a gene of interest may have expression that is neither cyc

223 For genes of interest, messenger RNA (mRNA) induction in car

224 a protocol in which the in vivo effects of a gene of interest on both B and T lymphocyte development

225 In order to generate HACs carrying a gene of interest, one can either co-transfect the alphoi

226 to prepare a mouse model that expresses the gene of interest only in the liver, but not in other tis

227 Users can find a particular gene of interest or a cluster of genes that behave simil

228 e through which researchers can search for a gene of interest or groups of genes by Gene Ontology cat

229 umerous functional siRNA constructs from any gene of interest or pool of genes.

230 nctional relationships for their tissues and genes of interest or examine gene function and interacti

231 cloning sites, and inability to express the genes of interest or purify the protein products.

232 ataset can be used to explore DTU events for genes of interest or to find genes with specific DTU in

233 either co-transfect the alphoid DNA and the gene of interest, or one can clone both into a single ve

234 For a gene of interest, our method can select other genes that

235 that directs the inhibition of a particular gene of interest, potentially producing a specific pheno

236 ive gene expression presents the data of the gene of interest relative to some calibrator or internal

237 To infer transcription kinetics for a gene-of-interest, researchers commonly compare the distr

238 sence of double-stranded RNA homologous to a gene of interest results in specific degradation of the

239 For any input gene of interest, SAPTA gives a ranked list of potential

240 Users can retrieve elements near single genes of interest, search for enhancers that target repo

241 Investigators can interrogate genes of interest, search for the genes that show the st

242 ogists to assess connectivity among a set of genes of interest ('seed-genes') within a biological net

243 ency for clustering was found common for the genes of interest, significant variations in gene order

244 Other genes of interest significantly altered in the presence

245 Current strategies involve modifying a gene of interest such that a degradation peptide is adde

246 tic map is essential for cloning of specific genes of interest such as the sex determination gene and

247 physically or functionally with the initial gene of interest than have SSNC-based screens.

248 Second, genes of interest that capture significant responses to

249 Genes of interest that discriminate CCS/MSP included tho

250 ge and data with a global F-test for finding genes of interest that minimizes the need for replicates

251 alter the level of mRNA transcribed from the gene of interest, the assumption is and always has been

252 For the 170 genes of interest, the DNA coverage was greater than 20x

253 For genes of interest, the user can view baseline expression

254 ntify multiple gene effects in two candidate genes of interest--the serotonin transporter gene (SLC6A

255 Of these, half do not impact the candidate gene of interest; the other half correlate with expressi

256 medical researchers with new tools to filter genes of interest, thus, reducing costly lab studies.

257 y 70 kb of alphoid DNA to any BAC carrying a gene of interest to generate a HAC vector.

258 uses viral vectors carrying a fragment of a gene of interest to generate double-stranded RNA, which

259 d were shown to be capable of delivering the gene of interest to target cells where it was translated

260 three case studies, ranging from querying a gene of interest to the identification of gene networks

261 idely used to 'knock-down' the expression of genes of interest to explore phenotypes.

262 particular gene and to link any two or more genes of interest to single species residing in complex

263 thod to easily make translational fusions of genes of interest to the lacZ reporter gene, under the c

264 he isolation of cDNA clones for a particular gene of interest, to the improvement of large gene colle

265 out), or multi-step recombineering to move a gene of interest under a repressible promoter (knockdown

266 results in near-immediate transcription of a gene of interest under physiological conditions.

267 ing an in-frame tag at the N-terminus of the gene of interest under the control of its native promote

268 ally selected and result in placement of the gene of interest under the control of new regulatory ele

269 lective isolation of any genomic fragment or gene of interest up to 250 kb in size from complex genom

270 g functional sites or regions of a candidate gene of interest using multiple linked SNPs.

271 In most of these studies inactivation of the gene of interest was associated with either glucose into

272 g version of the repressor plasmid while the gene of interest was delivered in an integrating piggyBa

273 choriomeningitis virus (LCMV) to express two genes of interest was recently reported.

274 with targeted selection of mutations in the genes of interest, was successfully applied to the gene

275 f a series of mutations on the function of a gene of interest we have generated three vector series t

276 carrying a different gene drive disabling a gene of interest, we are able to create diploid strains

277 t DNase I hypersensitive sites surrounding a gene of interest, we carried out both loss- and gain-of-

278 ows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicom

279 h, DNA was extracted from dried blood spots, genes of interest were amplified, amplicons were subject

280 Genes of interest were disrupted in 5 to 15% of preselec

281 Over 90 genes of interest were identified, and 35 of 44 tested w

282 Many genes of interest were involved in biological functions

283 h Ct serovar E, and changes in expression of genes of interest were measured using RT-PCR, proteomic

284 evated hepatic transcriptional levels of the genes of interest were noted in congenic C57BL/6J-Ah(d)

285 The genes of interest were potently and readily silenced wit

286 Two of the most highly dysregulated genes of interest were stresscopin, a neuropeptide invol

287 Additional differentially expressed genes of interest were those involved in the cytolytic p

288 Within each patient, the 18 genes of interest were used to calculate scores for dist

289 transgenic mice that overexpress one of the genes of interest were used to reveal genes involved in

290 k in the Gene Ontology (GO): given a list of genes of interest, what are the best nodes of the GO to

291 rphisms in the 5' untranslated region of the gene of interest when carrying out antisense morpholino

292 nked with directly oriented loxP sites and a gene of interest, which are introduced into plastids by

293 re initiated with the sequence of the user's gene of interest, which is searched against a database o

294 of embryos carrying mutations in a targeted gene of interest, which should allow for rapid screening

295 or allele-selective phenotypic assays of the gene of interest while it remains expressed and regulate

296 rategy allows easy and efficient delivery of genes of interest whose expression levels require regula

297 ive selection, and can precisely replace the gene of interest with a reporter that allows for high-re

298 om the same promoter, DD-Cas9 with any other gene of interest without co-modulation of the latter.

299 igh, allowing for a complete deletion of the gene of interest, without undesirable illegitimate integ

300 t LCMV (rLCMV) expressing additional foreign genes of interest would open novel avenues for the study