ライフサイエンスコーパス: gene replacement

1 oceeded with adeno-associated virus-mediated gene replacement.

2 niculi, by reductive evolution and analogous gene replacement.

3 on for these cryptic signals other than V(H) gene replacement.

4 generated cry knock-out mutants (cry0's) by gene replacement.

5 ase with which it can be altered by targeted gene replacement.

6 and Hyb-deficient mutants were generated by gene replacement.

7 isition of behavioral function subsequent to gene replacement.

8 us, either of man or mouse, does not allow V gene replacement.

9 some mAb contain DNA segments indicative of gene replacement.

10 letion mutations were attempted via targeted gene replacement.

11 acement and by a new procedure, heterokaryon gene replacement.

12 1 in vivo, it was disrupted in Neurospora by gene replacement.

13 ctor form of the parasite by double targeted gene replacement.

14 5% of all antibody molecules are produced by gene replacement.

15 g (Tox2(-)) strains were created by targeted gene replacement.

16 positional cloning, or attempts at targeted gene replacement.

17 e (ODC) alleles has been created by targeted gene replacement.

18 targets to stimulate targeted mutagenesis or gene replacement.

19 Two Tetrahymena strains were created by gene replacement.

20 mutant defective in ORF2 was constructed by gene replacement.

21 ace suitable for the evaluation of enzyme or gene replacement.

22 of Pseudomonas aerugionsa was constructed by gene replacement.

23 ted into the genome of a wild-type strain by gene replacement.

24 followed by mitochondrial transformation and gene replacement.

25 agment with two Chi sites was sufficient for gene replacement.

26 by directed plasmid insertion into scpA and gene replacement.

27 anisms; therefore, CMT1X may be treatable by gene replacement.

28 letion mutations were attempted via targeted gene replacement.

29 efficient donors, both for insertion and for gene replacement.

30 we suggest that it can also be deployed for gene replacement.

31 which can result in targeted mutagenesis or gene replacement.

32 nerated a umps double knockout (DKO) line by gene replacement.

33 phoid-system mice by human cytokine knock-in gene replacement.

34 We generated a deletion of Chd3 by targeted gene replacement.

35 ouble-crossover recombination, thus creating gene replacements.

36 ered by the technical difficulty of specific gene replacements.

37 n mutants for nonessential genes by one-step gene replacements.

38 ction strategies are used to engineer single-gene replacements.

39 To demonstrate ura3-based gene replacement, a Deltabop strain was constructed by t

40 On gene replacement after knockdown, versions of betaCOP wi

41 approach can be used to efficiently generate gene replacements allowing for modulation of protein lev

42 inactivation of Streptococcus mutans htrA by gene-replacement also resulted in a reduced ability to w

43 o one has shown the ability to correct, with gene replacement, an inherent defect in bipolar cells (B

44 strains carrying these mutations by two-step gene replacement and by a new procedure, heterokaryon ge

45 null mutants were created by double targeted gene replacement and characterized.

46 Gene replacement and genome editing demonstrated that TA

47 Gene replacement and in-frame deletion mutants were crea

48 iew discusses traditional approaches such as gene replacement and neuroprotection and also new avenue

49 Combining precise gene replacement and next-generation sequencing, we quan

50 and one on CENP-N) to CENP-A stability using gene replacement and rapid protein degradation.

51 bedside along the paths of neuroprotection, gene replacement and stem cell-based regenerative paradi

52 clavulanic acid biosynthesis as indicated by gene replacement and trans-complementation analysis in S

53 od employs homologous recombination-mediated gene replacement and was used to construct a variety of

54 ained with a Deltaura3 strain constructed by gene replacement and with derivatives of this strain in

55 CRISPR/Cas9 plus rAAV targeted gene-replacement and introduction of allele-specific RNA

56 s with epidermolysis bullosa following BPAG2 gene replacement, and can be used to identify interventi

57 s characterized by effectively instantaneous gene replacement, and the other consists of genes with f

58 EK2, was constructed from B. abortus 2308 by gene replacement, and the sodC mutant exhibited much gre

59 These results pave the way towards a gene replacement approach for OPMD treatment.

60 A site-directed gene replacement approach was used to isolate a cymA kno

61 omings may greatly limit the utility of this gene replacement approach.

62 thine phosphoribosyltransferase (XPRT) using gene replacement approaches were not successful, lending

63 of new or improved prodrug/suicide systems, gene replacement approaches, or strategies targeting the

64 tuated Chi sites, 5' GCTGGTGG 3', stimulated gene replacement approximately 50-fold, more than the su

65 Although clinical trials of gene replacement are in the earliest stages, this treatm

66 Viral gag gene replacements are influenced by host restriction gen

67 introduced into the strains by DNA-mediated gene replacement, are viable at 37 or 42 degrees C witho

68 ontinuous L-DOPA delivery in the striatum by gene replacement as a model for a gene therapy for Parki

69 We investigated gene replacement as a strategy for restoring inner ear f

70 PDZ domain blocked both tethering and, in a gene replacement assay, Golgi ribbon formation.

71 ufficient to unlink the Golgi apparatus in a gene replacement assay.

72 engineering in mouse cells, in concert with gene replacement assays to prove the functional signific

73 ad to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies.

74 hila, we applied two strategies for ends-out gene replacement at the endogenous yellow (y) locus in D

75 tituted strain of man1 was constructed using gene replacement at the native locus.

76 Gene replacement at this locus was achieved via homologo

77 ) vectors are considered promising for human gene replacement because they facilitate stable expressi

78 as shown to be a potent barrier to efficient gene replacement, but its effect was considerably amelio

79 strate that the outcome can be biased toward gene replacement by disabling the major NHEJ pathway and

80 dTALENs stimulated high rates (up to 34%) of gene replacement by homologous recombination.

81 utagenesis, chemical mutagenesis, homologous gene replacement, conditional knockdown techniques, and

82 SOR1 null mutants generated via targeted gene replacement confirmed the requirement for SOR1 in p

83 tudy was an investigation of whether somatic gene replacement could rescue degenerating photoreceptor

84 A gene replacement/deletion Brucella oxyR mutant exhibits

85 ter chromatid exchange events and suppressed gene replacement, demonstrating the involvement of the p

86 unable to generate dhch1(-) null mutants by gene replacement, despite using a wide spectrum of nutri

87 Simple gene replacement did not rescue Skp1 glycosylation, wher

88 ene in the pathway, by gene complementation, gene replacement, DNA sequence, and Northern hybridizati

89 olve knock-in (reporters or recombinases) or gene replacement (e.g., conditional knockout alleles con

90 ANA clone was generated by SHM after a V(H) gene replacement event.

91 tant S. globisporus M12 was prepared through gene replacement experiment of lndM2.

92 Gene replacement experiments show that RhoBTB3 function

93 available for M. lewisii will enable gene-by-gene replacement experiments to dissect the genetic and

94 Gene-replacement experiments demonstrated that injury tr

95 ouble targeted gene replacement or by single gene replacement followed by negative selection for loss

96 y positive data in clinical trials including gene replacement for Hemophilia B, X-linked Severe Combi

97 that may serve as useful targets for cell or gene replacement for pulmonary disorders.

98 al and comparative genomics, in diagnostics, gene replacement, generation of animal models for human

99 Disrupting omcB or omcC by gene replacement had no impact on growth with fumarate.

100 Targeting BCs with gene replacement has been difficult primarily due to the

101 f our knowledge, this is the first time that gene replacement has been reported for this type of fung

102 Gene replacement has now been used to produce mice expre

103 is the target for the myelin-derived signal, gene replacement has now been used to produce mice in wh

104 INTERPRETATION: Voretigene neparvovec gene replacement improved functional vision in RPE65-med

105 Employing a method to rapidly induce exact gene replacement in budding yeast [6], we show here that

106 Gene replacement in dystroglycan-deficient muscle demons

107 This technique allows for gene replacement in E. coli without prior cloning of the

108 oley's Anemia (CA) was generated by targeted gene replacement in embryonic stem (ES) cells.

109 in place of the mouse Apoe gene via targeted gene replacement in embryonic stem cells.

110 esent a successful restoration of hearing by gene replacement in mice, which is a significant advance

111 cessful use of a suicide vector for directed gene replacement in MR-1.

112 inger nucleases (ZFNs) has been deployed for gene replacement in plant cells.

113 Targeted gene replacement in plastids was used to explore whether

114 An improved method for gene replacement in Pseudomonas aeruginosa was developed

115 Gene replacement in rd1 mice that are devoid of the muta

116 se 1 studies have shown potential benefit of gene replacement in RPE65-mediated inherited retinal dys

117 DNA sequence mismatches on the frequency of gene replacement in Saccharomyces cerevisiae.

118 y combining chemical genetics and homologous gene replacement in somatic cells, we reveal different m

119 Using mutagenesis and homologous gene replacement in Tetrahymena thermophila, we analyzed

120 ts suggest that persistence of BAAV-mediated gene replacement in the cochlea is limited by the extens

121 ing a phage mediated delivery system and the gene replacement in the mutant was confirmed by polymera

122 background, and phenocopied the mutation by gene replacement in the WT strain.

123 paired the effectiveness of tumor suppressor gene replacement in treating metastases.

124 e RPB1 gene via homologous recombination and gene replacement in vivo.

125 HGPRT and/or APRT were generated by targeted gene replacement in wild type cells and preexisting muta

126 in the loss of embryonic viability based on gene replacements in C. elegans.

127 an efficient procedure for creating precise gene replacements in the cosmid clones by using PCR targ

128 c obliteration of the XPRT locus by targeted gene replacement indicated that XPRT was not an essentia

129 atidylinositol 4-kinase activity, and direct gene replacement indicates that STT4 is the defective ge

130 These results show that gene replacement is a feasible treatment strategy for th

131 Gene replacement is a logical strategy for ABCA4-associa

132 generic conjugation into Streptomyces, where gene replacement is selected.

133 nsporter genes have been deleted by targeted gene replacement, is unable to replicate as amastigote f

134 A series of stable gene replacement lines were generated that carried point

135 ine that spontaneously undergoes serial V(H) gene replacement mediated by cryptic recombination signa

136 a fast cost-effective lentivirus-based rapid gene replacement method to interrogate the physiopatholo

137 A new gene replacement method was used to disrupt the amyA cod

138 study confirmed previous reports that APOE4 gene replacement mice were less sensitive than APOE3 mic

139 tumor suppressor, we created a new knock-in gene replacement mouse model in which the endogenous Trp

140 anatomical targets and proper timing of the gene replacement must be understood.

141 ted through successive cycles of chromosomal gene replacement mutagenesis.

142 mentary yhcS or yhcR, no viable yhcS or yhcR gene replacement mutant was recoverable.

143 The ABC1 insertional mutant and a gene-replacement mutant arrest growth and die shortly af

144 with necrotizing fasciitis, or with isogenic gene replacement mutants deficient in cysteine protease,

145 es a simple approach for the construction of gene replacement mutants in both slow- and fast-growing

146 In addition, a gene replacement mutation was constructed for a gene enc

147 Saccharomyces cerevisiae by exact endogenous gene replacement of CDH1 with CDK-unphosphorylatable CDH

148 Gene replacement of MoGSK1 caused significant delay in m

149 Also, targeted gene replacement of PDX2 in C. nicotianae results in pyr

150 wever, cells containing a precise endogenous gene replacement of SIC1 with SIC1-0P (all nine phosphor

151 Through targeted gene replacement of the endogenous yellow gene, we show

152 Cooley anemia (CA) was generated by targeted gene replacement of the mouse adult globin genes in embr

153 s wild-type chloroperoxidase, thus requiring gene replacement of the wild type by the mutant gene.

154 application of site-directed mutagenesis and gene replacement of Thermus thermophilus rpsL to assess

155 Gene replacement of wild-type TOA2 with a W76E or Y69A/W

156 ii) modify an assembled metabolic pathway by gene replacement or addition.

157 py recognized two very different approaches: gene replacement or augmentation and gene repair.

158 outs were obtained either by double targeted gene replacement or by single gene replacement followed

159 Gene replacement or gene reactivation therapies for sick

160 animals and plants, in applications such as gene replacement or population suppression of pest speci

161 r these proteins in suppressing an alternate gene replacement pathway in which incorporation of both

162 AAV-mediated RPGRIP gene replacement preserves photoreceptor structure and f

163 We describe a posttranscriptional gene replacement (PTGR) approach where endogenous bialle

164 dariomyces fumago expression system by using gene replacement rather than gene insertion technology.

165 me was specifically altered (gene knockouts, gene replacements, reordering of genetic elements) such

166 Gene replacement represents a strategy for correcting th

167 r mutation of Ile8 to alanine via preprotein gene replacement resulted in a 4-fold and 2-fold drop in

168 resistance cassette introduced into icmS by gene replacement resulted in a mutant that was attenuate

169 sely resemble the PLCBc active site, while a gene replacement resulted in L. monocytogenes secreting

170 new mutants and most of these were targeted gene replacements resulting from homologous recombinatio

171 acterization of an ampA- strain generated by gene replacement reveals a significant increase in cell-

172 in the development of a general PCR-mediated gene replacement scheme for Escherichia coli.

173 Targeted gene replacement showed that MgAPT2 is required for both

174 ures and cuticle penetration, and a targeted gene replacement showed that the gene is required for fu

175 ne Chi-one exchange hypothesis ("long chunk" gene replacement), stemming from studies with purified R

176 Isogenic Xcc fliC gene replacement strains expressing eliciting or nonelic

177 . eutropha phaC PHA synthase and phaP phasin gene replacement strains were constructed.

178 The screen uses yeast gene-replacement strains depending for growth on cloned

179 Yeast gene-replacement strains depending for growth on the exp

180 The highly sensitive yeast gene-replacement strains described here provide a conven

181 ticular noted advances in using conventional gene replacement strategies, RNA-based technology and ph

182 Genome editing improves on simple gene-replacement strategies by effecting in situ correct

183 an archaeon, we have developed a homologous gene replacement strategy for Halobacterium salinarum ba

184 Using a transgenic gene replacement strategy in a Pitx1 mutant mouse, we ha

185 By using a gene replacement strategy in which C/EBPbeta was express

186 letion of the SOWgp gene by using a targeted gene replacement strategy resulted in partial loss of th

187 We have used a gene replacement strategy to generate mice that express

188 in (ETRA-153) was isolated from MR-1 using a gene replacement strategy.

189 investigated the function of FgVps27 using a gene replacement strategy.

190 Here, we use a gene-replacement strategy at the calyx of Held, a large

191 Of importance to any gene-replacement strategy for treatment of CF is the ide

192 tations in the genome using a phage-P1-based gene-replacement strategy.

193 Gene replacement studies in mice indicate that the devel

194 Using gene replacement studies, we demonstrate that the thiore

195 The finding of this gene replacement study in LGMD2D has important implicati

196 The findings of this gene replacement study in LGMD2D subjects have important

197 In this regard, a recent gene replacement study revealed that the phosphorylation

198 This is the first gene replacement study to report long-term rescue of a p

199 , but not both, can be disrupted by targeted gene replacement, suggesting that TbVCP is essential for

200 protective P. aeruginosa vaccine, we used a gene replacement system based on the Flp recombinase to

201 We developed a gene replacement system using the rpsL gene of Streptomy

202 The gene replacement technique described here has been used

203 We used a site-directed gene replacement technique to introduce an allele of ds2

204 jor (LmNcb5or) knock-out mutants by targeted gene replacement technique.

205 essential for metronidazole susceptibility, gene replacement technology has been developed for T. va

206 Therefore, by gene replacement technology, we engineered mice to expre

207 Targeted gene replacement (TGR) in yeast and mammalian cells is i

208 Targeted gene replacement (TGR) using fragments generated by PCR

209 'Targeted gene replacement' (TGR) resulting from homologous recomb

210 single insertions at targeted loci (targeted gene replacements, 'TGR') occur with a frequency of 7-20

211 Gene replacement therapies utilizing adeno-associated vi

212 ong-term survival of transduced cells during gene replacement therapies.

213 approach provides a potential alternative to gene replacement therapies.

214 ssment of new therapeutic approaches such as gene replacement therapy and pharmacological treatments.

215 Our findings establish that MEN1 gene replacement therapy can generate menin expression i

216 pemphigoid as well as individuals undergoing gene replacement therapy for epidermolyis bullosa.

217 To evaluate the feasibility of gene replacement therapy for GSD-1a, we have infused ade

218 dies is a serious complication of protein or gene replacement therapy for hemophilias, congenital X-l

219 ent findings should facilitate the design of gene replacement therapy for RPGR-null mutations.

220 Gene replacement therapy for RPGR-XLRP was hampered by t

221 ential for application of nanoparticle-based gene replacement therapy for treatment of human retinal

222 Gene replacement therapy has met with great difficulty b

223 inant adeno-associated virus (rAAV)-mediated gene replacement therapy in animals and humans with an i

224 t the potential for gene delivery to BCs and gene replacement therapy in human CSNB1.

225 Previous research utilizing Mertk gene replacement therapy in RCS rats provided proof of c

226 We have thus established the potential of gene replacement therapy in varying rates of degeneratio

227 treating a variety of red cell disorders by gene replacement therapy including severe beta-thalassem

228 Gene replacement therapy is a promising strategy for tre

229 We evaluate whether AAV-mediated gene replacement therapy is able to improve photorecepto

230 Gene replacement therapy is an attractive approach for t

231 Gene replacement therapy is complicated by the risk of a

232 cy in blood coagulation factor IX (F.IX)] by gene replacement therapy is hampered by the risk of immu

233 These results indicate that gene replacement therapy may be effective in patients wi

234 transcription factors (i.e., HIF-alpha) and gene replacement therapy of tumor suppressor genes (i.e.

235 clinical applications include mitochondrial gene replacement therapy to prevent transmission of mtDN

236 We used adeno-associated virus-2-based RPE65 gene replacement therapy to treat three young adults wit

237 retinal function in Aipl1 h/h mice following gene replacement therapy using an AAV2/2 vector and in t

238 previously examined the short-term effect of gene replacement therapy using an adeno-associated (AAV)

239 Using this model, we evaluated if gene replacement therapy using recent advancements in ad

240 patients with AIPL1 mutations suggests that gene replacement therapy will likely have to be performe

241 Enzyme or gene replacement therapy with acid alpha-glucosidase (GA

242 We demonstrate that rAAV-mediated gene replacement therapy with different forms of the hum

243 pivotal role in monitoring cell trafficking, gene replacement therapy, protein-protein interactions,

244 ned and used to ascertain the feasibility of gene replacement therapy, stem cell transplantation, and

245 seases are especially attractive targets for gene replacement therapy, which appears to be clinically

246 USH treatment trials, such as MYO7A somatic gene replacement therapy.

247 s in the ABCA4 gene are being considered for gene replacement therapy.

248 plant can theoretically also be treated with gene replacement therapy.

249 f GAN neurons, supporting the feasibility of gene replacement therapy.

250 ong the BEST1-related ocular conditions, for gene replacement therapy.

251 The development of gene-replacement therapy for inborn errors of metabolism

252 agella, as verified with a mutant with a hag gene replacement; this latter finding highlights the imp

253 ed gene-targeting vector, they can stimulate gene replacement through both homology-directed and homo

254 placed by ADE1, preventing simple homologous gene replacement to become Leu2(+).

255 This study has used the strategy of gene replacement to characterize the contribution of the

256 plement to gene deletion would be subsequent gene replacement to demonstrate re-acquisition of functi

257 Here, we used mutant combinations and gene replacement to determine the constraints of ligand

258 Gene replacement to express mutated H3 variants reveals

259 flow cytometry in conjunction with cortactin gene replacement to identify C-terminal tyrosines, the f

260 he pipeline are appraised, ranging from SMN1 gene replacement to modulation of SMN2 encoded transcrip

261 In this issue, Li et al. use gene replacement to produce mice expressing a Cdc20 muta

262 We have used in vitro mutagenesis and gene replacement to study the function of the nucleotide

263 mutagenesis of lysine residues, coupled with gene replacement, to identify the sites of acetylation o

264 5 has attained greater significance now that gene replacement trials have begun.

265 ck-in model of NDI was generated by targeted gene replacement using a Cre-loxP strategy.

266 S III active site region and was isolated by gene replacement using a medium supplemented with a sour

267 We studied gene replacement using a related method of gene transfer

268 ent an efficient method for gene tagging and gene replacement using Cre recombinase-mediated cassette

269 that accompanies the lambda Red proteins for gene replacement using recombineering technology.

270 The argR gene was inactivated by gene replacement, using a gentamicin cassette.

271 henomena of language replacement and also of gene replacement, usually partial, due to gene flow.

272 E. coli chromosome by using plasmid pKO3, a gene replacement vector that contains a temperature-sens

273 Two vectors were tested: a gene-replacement vector derived from the A component; an

274 Eukaryotic gene targeting by means of gene replacement vectors is often complicated by unwante

275 The method employs several new gene replacement vectors that incorporate (1) the counte

276 re-sensitive SC101 plasmids may be useful as gene replacement vectors.

277 through nonhomologous end joining (NHEJ) and gene replacement via homologous recombination (HR) are s

278 onhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have

279 A 2.7 kilobase (kb) homozygous gene replacement was achieved in up to 11% of iPSC witho

280 Gene replacement was dependent on RecA and RecB function

281 The efficiency of gene replacement was extraordinarily high, allowing for

282 Gene replacement was facilitated by a selection protocol

283 This gene replacement was initially done for wild-type (wt) r

284 ion within a single genomic domain, targeted gene replacement was used to exchange the endogenous yel

285 In this study, targeted gene replacement was used to obtain evidence that TR is

286 Using gene replacement, we altered two core promoter elements

287 Using gene replacement, we demonstrate that introduction of th

288 By gene replacement, we have isolated archaeal mutants of T

289 To study sarcoglycan gene replacement, we introduced transgenes expressing mu

290 Gene replacements were made to exchange the alleles foun

291 and we constructed another Rbs(-) strain by gene replacement with a deletion not involving IS150.

292 The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment.

293 of ibeA (ZD1) was constructed by chromosomal gene replacement with a suicide plasmid pCVD442 carrying

294 sequences, indicating that the inhibition of gene replacement with mismatched sequences is at least p

295 rl1p (Granule lattice protein 1) by complete gene replacement with modified alleles.

296 o spermidine, was created by double-targeted gene replacement within a virulent L. donovani backgroun

297 iosynthesis, were created by double targeted gene replacement within a virulent strain of L. donovani

298 o the generation of GFP knock-in alleles and gene replacements without co-integrated markers.

299 wo-step mutagenesis strategy that allows for gene replacements without introducing new selectable mar

300 e to haloxyfop and sethoxydim, rendering the gene-replacement yeast strains resistant and sensitive t