ライフサイエンスコーパス: gene targeting

1 and the desired target locus (aptamer-guided gene targeting).

2 quantitative and qualitative nature of STAT-gene targeting.

3 erapies and limit the efficacy of individual gene targeting.

4 st, Cas9/dCas9-PB chimeras did not result in gene targeting.

5 acile vectors for high-efficiency, multiplex gene targeting.

6 onstrated a high-efficiency in site-specific gene targeting.

7 or resistance to DNA damaging agents and for gene targeting.

8 n cells, often limiting the effectiveness of gene targeting.

9 nt of the TIN2 K280E DC allele (TIN2(DC)) by gene targeting.

10 rry either human COMT Val or Met alleles via gene targeting.

11 ndwork for future studies using aptamers for gene targeting.

12 ransgene to achieve collecting duct-specific gene targeting.

13 , and causation was confirmed by CRISPR/Cas9 gene targeting.

14 of specific epigenetic marks and peripheral gene targeting.

15 ne TALE-PB chimera demonstrated notable HPRT gene targeting.

16 ring technology with the ease of multiplexed gene targeting.

17 cific DNA double-strand break, and stimulate gene targeting.

18 for evolution and potential applications in gene targeting.

19 transgene into one copy of chromosome 21 by gene targeting.

20 into the 3'-untranslated region of C5ar1 by gene targeting.

21 ens when both HY2 and PUBS were disrupted by gene targeting.

22 ebrafish using zinc-finger nuclease-mediated gene targeting.

23 fic TRAF6 deficiency using Cre/LoxP-mediated gene targeting.

24 BAergic inputs are challenged by conditional gene targeting.

25 es even 1 year after tamoxifen-induced Cox10 gene targeting.

26 role of GSK-3alpha in the mouse heart using gene targeting.

27 ogy, we established S100A4-deficient mice by gene targeting.

28 oma cells were all successfully corrected by gene targeting.

29 Knock-in mice were generated by gene targeting.

30 ed genome rearrangements, and to DSB-induced gene targeting.

31 a safe harbor suitable for nuclease mediated gene targeting.

32 ements in mRNAs are leveraged as signals for gene targeting.

33 us tropicalis which is used for genetics and gene targeting.

34 G3 were disrupted using CRISPR/Cas9-mediated gene targeting.

35 y was sensitive enough to evaluate multiplex gene targeting.

36 strand break, which has been used to promote gene targeting.

37 method can be used for efficient multiplexed gene targeting.

38 floxed mice that can be used for conditional gene-targeting.

39 analysis as a consequence of miRNA-directed gene targeting, a phenomenon that could be exploited to

40 The level of gene targeting achieved was sufficient to correct the pr

41 single-guide RNAs (sgRNAs) and from limited gene targeting activity.

42 diploid organism, which requires sequential gene targeting, allows dissection of virulence mechanism

43 acrophages from Gsdmd(-/-) mice generated by gene targeting also exhibit defective pyroptosis and int

44 Cultured NPCs are amenable to gene targeting and can form nephron organoids that engra

45 of 53BP1 at sites of DNA damage and improved gene targeting and chromosomal gene conversion with eith

46 We used gene targeting and computational modeling to test this n

47 monstrated that STAT5 content is crucial for gene targeting and EPC fate.

48 Light Reporter, which provides a readout of gene targeting and gene disruption downstream of a targe

49 s out single-strand annealing in addition to gene targeting and gene disruption.

50 ctors makes them important also as tools for gene targeting and genome editing.

51 Over the last 20 years, however, advances in gene targeting and genome modification in rodent models

52 We combined ChIP methods with conditional gene targeting and mRNA expression analysis and identifi

53 e IDH1 mutation and ACACA in leukaemia using gene targeting and patient-derived xenografts.

54 stem can be exploited as a powerful tool for gene targeting and precise genome editing in plants.

55 sing retroviral vectors are low frequency of gene targeting and random integration of the targeting v

56 By using somatic gene targeting and recombinase-mediated cassette exchang

57 Human MPS1-null cells were generated via gene targeting and reconstituted with either the wild-ty

58 Using gene targeting and RNAi, we showed that depletion of the

59 of Cell Stem Cell, Pak et al. (2015) combine gene targeting and stem cell technologies to identify a

60 t and convenient alternative to conventional gene targeting and will greatly facilitate the rapid cre

61 Live imaging, gene targeting, and cell-cycle inhibitors reveal that cl

62 ential clonal events: initial reprogramming, gene targeting, and subsequent removal of a selection ca

63 Rgs6(-/-) mice were generated by gene targeting, and the cardiac effects of Rgs6 ablation

64 tein engineering-many are unsatisfactory for gene targeting applications.

65 efined factors could spur the development of gene-targeting applications in non-dividing cells.

66 diated oral delivery of RNase P ribozyme for gene-targeting applications in vivo.

67 gonal CRISPR-Cas systems in rapidly emerging gene-targeting applications.

68 ase P ribozymes as an effective approach for gene-targeting applications.

69 To test this possibility, we used a gene targeting approach to determine whether the closely

70 inant Adeno-Associated Virus (rAAV) mediated gene targeting approach to introduce allele-specific mut

71 te the role of BAP1 in DSB repair, we used a gene targeting approach to knockout (KO) this deubiquiti

72 To explore these questions, we used a gene targeting approach to specifically ablate the G-pro

73 Using a Cre/loxP-mediated conditional gene targeting approach, Smad4 gene function was deleted

74 Using an in vivo gene targeting approach, we demonstrate that Rac2, but n

75 Using the conditional mouse gene targeting approach, we show that mice with inactiva

76 Hoxa5 expression domains using a conditional gene targeting approach.

77 Here, we developed a highly efficient gene-targeting approach in the monarch using zinc-finger

78 Here, we employ a novel gene-targeting approach that exploits a specific chromat

79 ce that lack kinase activity using a knockin gene-targeting approach that preserves normal protein ab

80 In the present study, by using the gene-targeting approach to delete TNFRII in AD transgeni

81 We used a conditional gene-targeting approach to identify the relative contrib

82 Using a gene-targeting approach, we knocked-in a single copy of

83 Using a gene-targeting approach, we observed that in contrast to

84 temness and pluripotency of murine ESCs by a gene-targeting approach.

85 Through gene targeting approaches in zebrafish and mice, we show

86 Analysis of PhIL1 function using gene targeting approaches indicated that the protein is

87 Here we used inducible and stage-specific gene targeting approaches to elucidate the role of PDK1

88 tissue-specific and temporal transgenic and gene targeting approaches, as well as introduction of po

89 Although gene-targeting approaches and phenotype analysis have le

90 Traditional gene-targeting approaches in many experimental organisms

91 By using gain- and loss-of-function gene-targeting approaches, here we show that PTEN is cru

92 e history of HD and valuable information for gene-targeting approaches.

93 transcriptional complex but was crucial for gene targeting, as p21(Waf) gene promoter, unlike cyclin

94 eduled DNA synthesis and a CRISPR-Cas9-based gene-targeting assay.

95 stimulate homologous recombination-mediated gene targeting at a variety of loci, including genes kno

96 repair pathway, increases the efficiency of gene targeting at fin1+ to around 75-80% (a 16-fold incr

97 ly, we demonstrate that Cas9 nickases induce gene targeting at frequencies comparable to native Cas9

98 Both TALENs and CRISPR/Cas9 achieved gene targeting at similar efficiencies.

99 wever for some loci, such as fin1+, rates of gene targeting below 5% can limit the scope and scale of

100 uses Helper sites concurrently with WREs for gene targeting, but it also targets TCF1 to sites that d

101 Gene targeting by 'ends-out' homologous recombination en

102 Gene targeting by homologous recombination (HR) can be i

103 Gene targeting by homologous recombination or by sequenc

104 Gene targeting by repeated local administration of oligo

105 Gene targeting by zinc-finger nucleases in one-cell embr

106 The frequency of injection-mediated gene targeting can be further increased with CRISPR-indu

107 that multiplex embryo transfer and multiplex gene targeting can be used to quickly and efficiently ge

108 We have taken advantage of the gene targeting capability of the moss Physcomitrella pat

109 eted for REST in progenitors by conventional gene targeting does not exhibit these phenotypes, likely

110 ingly, the overexpression of IL-22 by either gene targeting (e.g., IL-22 transgenic mice) or exogenou

111 mal production, and sheds light on improving gene-targeting efficiencies on pluripotent stem cells.

112 l concept and research direction to increase gene targeting efficiency and lays the groundwork for fu

113 J with SCR7 does not increase HDR or improve gene targeting efficiency further, indicating that HR is

114 fector nuclease technology, we achieved high gene-targeting efficiency in AAT-deficiency patient iPSC

115 ng/discovery for liver diseases, and the low gene-targeting efficiency in human iPSCs warrants furthe

116 r targeting vector design will inform future gene targeting efforts involving multi-kilobase gene seg

117 Here we describe experiments using gene targeting, electrophysiology, and optical imaging t

118 simple, efficient and scalable way to enrich gene targeting events and to identify the cause of failu

119 10% of the injected F0 animals transmitting gene-targeting events through their germline.

120 t to be identified mediators of nick-induced gene targeting exist.

121 Although recent gene targeting experiments demonstrated that the presenc

122 Gene targeting experiments have shown that the cytokine

123 er cells phenocopies the effects observed in gene targeting experiments.

124 The present gene-targeting experiments demonstrate that mouse embryo

125 Gene-targeting experiments report that the homeodomain-i

126 Subsequently, CDK11 siRNA gene targeting, expression profiling, and network recons

127 matic cells, restricting the use of standard gene targeting for most laboratory and clinical applicat

128 in genetically engineered mouse models, and gene-targeting for the generation of knock-in mice.

129 arms and chromosomal target sequences reduce gene targeting frequencies in several species; however,

130 ased on the bean yellow dwarf virus enhanced gene targeting frequencies one to two orders of magnitud

131 The gene targeting frequencies varied by as much as 10-fold,

132 d base pair can significantly decrease human gene targeting frequencies.

133 nhance ZFN-mediated targeted mutagenesis and gene targeting (GT) in Arabidopsis by manipulating DNA r

134 The ability to edit plant genomes through gene targeting (GT) requires efficient methods to delive

135 c endonucleases boosted the establishment of gene targeting (GT) techniques in a row of different spe

136 Conditional gene targeting has been extensively used for in vivo ana

137 Although gene targeting has been used extensively to modify the g

138 ng engineered nucleases to stimulate precise gene targeting have also demonstrated correction of dise

139 Powerful methods of gene targeting have helped to decipher gene-function ass

140 ategies routinely use cDNA-based vectors for gene targeting; however, inclusion of noncoding componen

141 ic mosquitoes carrying antipathogen effector genes targeting human malaria parasites and to generate

142 We used gene targeting in a disease-susceptible rat model of gen

143 otein and short guide RNA, driving efficient gene targeting in a non-integrative manner.

144 cytes in vivo, Cre-loxP-mediated conditional gene targeting in adult mice was used.

145 TALEN-mediated gene targeting in avian PGCs is therefore an efficient p

146 More generally, gene targeting in avian primordial germ cells will foste

147 is method may allow for safe and efficacious gene targeting in both infants and adults by greatly dim

148 y of sophisticated genetic modifications via gene targeting in both mouse and human NSC lines, includ

149 finger nuclease (ZFN) technology facilitates gene targeting in diverse species and cell types, but an

150 Gene targeting in embryonic stem cells has become the pr

151 e to be a viable alternative to conventional gene targeting in embryonic stem cells where simple loss

152 The advantages of gene editing versus gene targeting in embryonic stem cells, including the br

153 -engineered strains made via transgenesis or gene targeting in embryonic stem cells.

154 Gene targeting in ES cells was used to create an EGFP kn

155 Here we provide a detailed protocol for gene targeting in human cells with AAV vectors.

156 mology-directed DNA repair pathway constrain gene targeting in human HSCs.

157 n iPSC-based disease model and highly robust gene targeting in human iPSCs, both of which are critica

158 ein (CDX2P-CreER(T2)) to allow for inducible gene targeting in intestinal epithelium.

159 caffeine treatment has been shown to prevent gene targeting in mammalian cells by increasing non-prod

160 oxa3 was the first Hox gene to be mutated by gene targeting in mice and is required for the developme

161 Studies of Brca2 mutation by gene targeting in mice are limited, given that homozygou

162 eloping recombinase-assisted and conditional gene targeting in mice as a main technical tool.

163 Gene targeting in mice subsequently demonstrated that th

164 within a single lineage, we used conditional gene targeting in mice to ablate Sin3a from perinatal qu

165 Here, we use gene targeting in mice to determine the requirement of G

166 In this study, we used gene targeting in mice to identify the in vivo functions

167 y for more efficient and less time-consuming gene targeting in mice using embryonic injection of zinc

168 Traditional gene targeting in mice using embryonic stem (ES) cells,

169 findings demonstrate the accuracy of in vivo gene targeting in modeling human cancer and suggest futu

170 Using conventional gene targeting in mouse embryonic stem cells, we report

171 cleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms.

172 ficity at two diverse targets, and stimulate gene targeting in multiple cell types including human in

173 ships of the different domains of DEK1 using gene targeting in null mutant background.

174 ntional methods are not readily adaptable to gene targeting in other cell types.

175 vercome the efficiency barrier that has made gene targeting in plants challenging.

176 system, which is widely used for conditional gene targeting in the mouse.

177 ng of p22(phox) reduces HIF-2alpha-dependent gene targeting in vitro and tumor formation in vivo.

178 blications have demonstrated the efficacy of gene targeting in zebrafish using CRISPR/Cas9, and they

179 r the mouse homologue of Inturned (Intu) via gene-targeting in ES cells.

180 Here we show that inactivation of Cul4a by gene-targeting in mice only affected male but not female

181 Custom endonuclease-based gene targeting involves two mechanisms of DNA repair: ho

182 Gene targeting is a genetic technique to modify an endog

183 Gene targeting is a protocol for introducing a mutation

184 Gene targeting is indispensible for reverse genetics and

185 This genetic correction strategy without gene targeting is potentially applicable to any dominant

186 is of hair follicle epithelial stem cells by gene targeting is well established, the molecular and ge

187 been established by cell type-specific il-10 gene targeting, it remains elusive to what extent IL-10

188 ily engineered and highly effective tool for gene targeting; it has considerable off-target effects i

189 Our findings demonstrate how precise gene targeting may be applied to correction of X-CGD usi

190 Both chemical- and gene-targeting-mediated inactivation of Hh signaling aug

191 genetic tool advantageous over conventional gene targeting methods.

192 Using in vitro and in vivo gene-targeting methods, we show that Ipo11 loss results

193 By characterizing a conditional gene targeting mouse model bearing T cell deletion of Rh

194 Prevalent gene targeting mouse models for assessing beta-cell prol

195 P1R12C (AAVS1) alleles in CD8(+) T cells and gene targeting of a GFP transgene cassette in >40% of CD

196 Zinc finger nuclease-mediated gene targeting of a single-copy gp91(phox) therapeutic m

197 This study shows that conditional gene targeting of beta-catenin in the dorsal neural fold

198 Gene targeting of betacyto-actin, but not gammacyto-acti

199 Recent studies by conditional gene targeting of cdc42 in mice have revealed its tissue

200 Here, we show that sequential gene targeting of embryonic stem cells can be used to yi

201 Yet no gene targeting of Erk1/2 in muscle fibers in vivo has be

202 e of the JCI, Beedle et al. used conditional gene targeting of Fktn, the gene responsible for Fukuyam

203 Conventional gene targeting of Jak2, creating a null allele, leads to

204 However, gene targeting of matrilin-1 in mouse did not lead to pr

205 Site-specific gene targeting of PSIP1 may have therapeutic potential f

206 In addition, gene targeting of t-FcepsilonRIbeta attenuated microtubu

207 Conditional gene targeting of the catalytic subunit of glutamate cys

208 arization, lipolysis, kinase activities, and gene targeting or knock-down approaches.

209 opmental arrest when individually ablated by gene targeting or knockdown.

210 imply eliminating a specific gene product by gene targeting or RNA interference.

211 ckground, unintended indirect effects of the gene targeting, or the minimal genetic interference of t

212 This work studies gene targeting patterns amongst miRNAs with differential

213 eport the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-t

214 Using a gene-targeting plasmid containing a loxP-flanked drug-re

215 sequencing using newly designed DEH 16S rRNA gene targeting primers.

216 etween this protocol and the classical mouse gene-targeting protocol include ES cell culture methods,

217 In this paper, we describe a gene-targeting protocol to knock in a GFP cassette into

218 Further, reprogramming and typical gene-targeting protocols can be readily performed on the

219 human CD4+ T cells raises the possibility of gene targeting PSIP1 combinatorially with CCR5 for HIV-1

220 termed 'meganucleases' that are employed as gene-targeting reagents.

221 Because gene targeting remains significantly enriched, relative

222 of the wild-type IDH1 allele in IMA cells by gene targeting resulted in an 87-fold decrease in cellul

223 Such pathogen gene-targeting RNAs represent a new generation of enviro

224 n activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimul

225 1 pathway genetically in mice by conditional gene targeting starting 8 weeks after spinal hemisection

226 ular vector construction and high-efficiency gene-targeting strategies have been combined to mutate g

227 ul use within antisense, antigene, and other gene-targeting strategies.

228 several disease-specific iPSCs using various gene-targeting strategies.

229 cells can be significantly influenced by the gene-targeting strategy and genetic background employed.

230 PGT in ductus arteriosus closure, we used a gene-targeting strategy to produce mice in which PGT exo

231 hippocampal tissue and designed a Dlg4/PSD95 gene-targeting strategy: a Dlg4/PSD95 zinc finger DNA-bi

232 n the AGRIN signaling pathway cause CMS, and gene targeting studies in mice confirm the importance of

233 ds to be considered in the interpretation of gene targeting studies in the RPE.

234 Recent mouse gene targeting studies of rhoA and its regulators/effect

235 Gene targeting studies revealed that Dicer1 is required

236 e-expressing mice as controls in conditional gene targeting studies.

237 Mouse gene-targeting studies have provided convincing evidence

238 Gene-targeting studies in mice have identified the essen

239 Consistent with in vivo gene-targeting studies in mice, both gene- and transcrip

240 r specificities and features, and along with gene-targeting studies, have also begun uncovering evide

241 se limitations, we designed a new, inducible gene-targeting system by introducing an in-frame nonsens

242 c nephropathy, we used an inducible Cre-loxP gene-targeting system that enabled genetic deletion of V

243 Using an adenovirus-mediated gene targeting technique, we attempted to create an HCT1

244 This ES cell-based gene-targeting technique allows sophisticated genetic mo

245 Herein, we used gene-targeting techniques to delete PAT in Plasmodium yo

246 o improve safety, including the emergence of gene targeting technologies for the treatment of PIDs.

247 were discovered in chickens, yet the lack of gene targeting technologies in birds has limited biomedi

248 significantly more efficient than any other gene-targeting technology that is currently available an

249 in (Hupki) mouse model was constructed using gene-targeting technology to create a mouse strain that

250 /Cas system has been adapted as an efficient gene-targeting technology with the potential for multipl

251 3-cre knock-in allele to perform conditional gene targeting, testing the GDNF coreceptors Gfra1 and R

252 sis, JAK/STAT signaling, we used conditional gene targeting to develop a fully penetrant small animal

253 ur knowledge, the first successful selective gene targeting to epithelial vs. mesenchymal cells in an

254 e generated humanized DISC1-Boymaw mice with gene targeting to examine the in vivo functions of the f

255 al feature of pericytes, we used conditional gene targeting to examine the von Hippel-Lindau/prolyl-4

256 the organization of taste circuits, we used gene targeting to express the transsynaptic tracer barle

257 Here we have used in vivo gene targeting to insert an enhancer-promoter element at

258 of Cdk2 inhibitory phosphorylation, we used gene targeting to make an endogenous Cdk2 knockin allele

259 f GalNAc-T6 in colon cancer, we used precise gene targeting to produce isogenic colon cancer cell lin

260 odel system using zebrafish transgenesis and gene targeting to provide an explanation for this phenot

261 odel system using zebrafish transgenesis and gene targeting to provide an explanation for this phenot

262 on specifies TRAV15/DV6 as a Vdelta, we used gene targeting to replace the promoter region of a TRAV1

263 ate whether this might be true, we performed gene targeting to selectively delete LXRalpha in hepatoc

264 For example, gene targeting to the ROSA26 locus by homologous recombi

265 We used gene targeting to validate candidate genetic factors.

266 cleavage reagents that have been adopted as gene-targeting tools.

267 tamer-containing oligonucleotides stimulated gene targeting up to 32-fold in yeast Saccharomyces cere

268 for his contributions to the development of gene targeting using homologous recombination in embryon

269 bout homology-directed repair (HDR)-mediated gene targeting using long donor DNA templates in hPSCs w

270 Homologous recombination-based gene targeting using Mus musculus embryonic stem cells h

271 Conditional gene targeting using the bacteriophage-derived Cre recom

272 fects of this mutation by using somatic cell gene targeting using the nontumorigenic human breast epi

273 lation needed to make a conditional knockout gene targeting vector to only two steps: a single round

274 Using a gene-targeting vector or a synthetic oligodesoxynucleoti

275 co-delivered with an appropriately designed gene-targeting vector, they can stimulate gene replaceme

276 recombineering tool kit for generating mouse gene targeting vectors and demonstrate the advantage of

277 rovides a tool for the design and display of gene targeting vectors in the same genome browser, along

278 d expansion of rat ES cells, construction of gene-targeting vectors, generation of gene-targeted rat

279 produced through IVF, and a high rate of Fah gene targeting was achieved with microinjection of Cas9

280 nuclease-mediated DNA double-strand breaks, gene targeting was promoted by replication of the repair

281 Conditional gene targeting was used in mice to explore the role of P

282 Using somatic cell gene targeting, we demonstrated that a heterozygous BRCA

283 Using mouse models of Raptor or Rictor gene targeting, we discovered that Rictor ablation inhib

284 Using conditional gene targeting, we found that GC-specific inactivation o

285 Using efficient gene targeting, we generated a precise PpDEK1 deletion (

286 By efficient gene targeting, we have replaced, en masse, the promoter

287 By gene targeting, we introduced a point mutation (W1206R)

288 Here, using conditional gene-targeting, we show that the canonical mediator of T

289 Molecules that elevate HDR may improve gene targeting whereas inhibiting molecules can be used

290 However, gene targeting, which is crucial for functional studies

291 he RUNX1 mutation in 1 FPD iPSC line through gene targeting, which led to normalization of megakaryop

292 he Nobel Prize in medicine for their work on gene targeting, which showed that embryonic stem cells c

293 PpRAD50 strongly and specifically inhibited gene targeting, whilst rates of untargeted transgene int

294 Gene targeting with adeno-associated virus (AAV) vectors

295 Here we combined gene targeting with an auxin-inducible degradation syste

296 ence-specific, but also quadruplex specific, gene targeting with PNA probes.

297 yme function, and validates the use of GALNT gene targeting with SimpleCells for broad discovery of d

298 ssociated virus (rAAV)-mediated promoterless gene targeting without nucleases and demonstrate amelior

299 ZFN-mediated gene targeting yields high gene modification efficiencie

300 Conventional embryonic stem cell (ESC)-based gene targeting, zinc-finger nuclease (ZFN) and transcrip