ライフサイエンスコーパス: general acid catalysis

1 for the hydrolysis of mNBP, consistent with general acid catalysis.

2 yl substrates and a His-Glu pair involved in general acid catalysis.

3 ve charge on the leaving group and efficient general acid catalysis.

4 cates that these reactions involve only weak general acid catalysis.

5 roles in transition state stabilization and general acid catalysis.

6 n a Bronsted plot that was characteristic of general acid catalysis.

7 he active phosphatase conformation, enabling general acid catalysis.

8 ism of PPT is likely to involve general base-general acid catalysis.

9 ge residue Trp354 to Ala completely disables general acid catalysis.

10 ntermediate is subject to both specific- and general-acid catalysis.

11 l acetate ester was studied with and without general-acid catalysis.

12 ed MKP3 clearly indicated the involvement of general acid catalysis, a hallmark of protein-tyrosine p

13 olves transition-state stabilization through general-acid catalysis and freeing of three water molecu

14 n movement of the flexible loop that enables general acid catalysis are presented.

15 gainst other enzymatic KIEs with and without general acid catalysis, are consistent with a loose tran

16 g against a role for rescuing nucleobases in general acid catalysis, because a nucleobase that contri

17 he transition state for cleavage of 1-F from general acid catalysis by 0.80 M cyanoacetate buffer at

18 olate leaving group (Cys-35) via an enforced general acid catalysis by trapping mechanism.

19 p-99 in the catalytic diad which strengthens general acid catalysis by Tyr-14.

20 s, provide support for a mechanism involving general-acid catalysis by a conserved adenine residue in

21 o estimate the individual rate constants for general-acid catalysis by the diacid and monoacid forms

22 rom Yersinia, we have examined the effect on general acid catalysis caused by mutations to two conser

23 We established the requirement of general acid catalysis for E-P formation in reactions wi

24 hydroxide ion on the scissile phosphate, and general acid catalysis for protonation of the leaving 3'

25 s from previous studies that Cdc25A utilizes general acid catalysis for substrates with a leaving gro

26 acidic system reacted by means of concerted general acid catalysis (found to be a so-called D(N)A(N)

27 General acid catalysis in protein tyrosine phosphatases

28 lysis, because a nucleobase that contributes general acid catalysis in the cleavage pathway should pr

29 suggest a general base mechanism with likely general acid catalysis in the oxidative decarboxylation

30 ion to assess the functional significance of general acid catalysis in the system.

31 Our results show that the process of general acid catalysis is complex and suggest that Lys-1

32 In the Phe mutant, general acid catalysis is partially effective, but the p

33 General acid catalysis is rendered inoperative by the Ly

34 Biochemical experiments suggest that general acid catalysis may occur through the N3 position

35 Furthermore, the prediction that general acid catalysis may only be effective in low diel

36 ion provides an explanation for the impaired general acid catalysis observed in kinetic experiments w

37 f acetic acid and the imidazolium ion showed general acid catalysis of 18.5 and 1.5 M-1 sec-1, respec

38 nt with the conclusion that Glu-461 provides general acid catalysis of leaving group departure, which

39 bond fission with the alkyl substrate, while general acid catalysis of pNPP by YopH is more synchrono

40 This observation supports Glu19-CO(2)H general acid catalysis of the formation of mutant.III.

41 e Tyr-274 on the scissile phosphodiester and general-acid catalysis of the expulsion of the 5'-deoxyr

42 N (CHI.CHN) and CHI.TS, we found: (i) Lys-97-general-acid catalysis of the O2'(-) nucleophilic additi

43 ired for phosphodiester cleavage, either for general acid catalysis or for electrostatic stabilizatio

44 Ph appears to incorporate a lesser degree of general-acid catalysis, relative to the 2,3-isomer.

45 rformed to study the proton-transfer step in general acid catalysis that is facilitated by the cataly

46 Even without general acid catalysis, the D262N mutant reaction is act

47 rimental data that points to a novel mode of general acid catalysis through the N3 position of an ade

48 A1 performs general acid catalysis while G33 acts as a general base.