ライフサイエンスコーパス: genetic engineering

1 ntially powerful class of tools for targeted genetic engineering.

2 T cells, and its translation to therapeutic genetic engineering.

3 iple-step biosynthetic pathways using simple genetic engineering.

4 ting H. pylori mutant construction and other genetic engineering.

5 ction, or for practical applications through genetic engineering.

6 scovery paved the way for the development of genetic engineering.

7 gineering, either using natural variation or genetic engineering.

8 fixation, the use of fluorescent markers, or genetic engineering.

9 FNs) are powerful tools for gene therapy and genetic engineering.

10 icity concerns, plant disease treatment, and genetic engineering.

11 ld be attenuated at the early liver stage by genetic engineering.

12 osynthetic enzymes with proteins obtained by genetic engineering.

13 gi using both environmental manipulation and genetic engineering.

14 broader application to all cells amenable to genetic engineering.

15 l as hypothesis-driven experiments utilizing genetic engineering.

16 physical separation from wild relatives and genetic engineering.

17 human biopharmaceuticals in egg whites using genetic engineering.

18 ght lead to enhanced crop protection through genetic engineering.

19 rve as the basis for revolutionary tools for genetic engineering.

20 onsequently, Rubisco is a primary target for genetic engineering.

21 leaves of carrot (Daucus carota) via plastid genetic engineering.

22 nd allows component reusability with minimal genetic engineering.

23 of carotenoid production in crop plants via genetic engineering.

24 ge (SBOL) Visual is a graphical standard for genetic engineering.

25 elopment as a powerful and versatile tool in genetic engineering.

26 d guided improvements via in-silico-assisted genetic engineering.

27 ia, and thus constitutes a powerful tool for genetic engineering.

28 d therefore represent a difficult target for genetic engineering.

29 utation is recapitulated in a mouse model by genetic engineering.

30 ur have largely relied upon various forms of genetic engineering.

31 With the advent of genetic engineering, a highly functional population of T

32 making these enzymes interesting targets for genetic engineering aimed at increased hydrogen producti

33 biological methods such as systems biology, genetic engineering and bio-refining for optimizing alga

34 Finally, genetic engineering and cell-specific application of ext

35 Genetic engineering and DNA manipulation techniques enab

36 f microbial evolution, interaction networks, genetic engineering and drug discovery.

37 Genetic engineering and evolutionary adaptation to incre

38 er system should be broadly useful for plant genetic engineering and functional genomics.

39 es of the PB system for applications such as genetic engineering and gene therapy.

40 imultaneous, an effect with implications for genetic engineering and horizontal gene transfer.

41 Today, purification of microbial elements, genetic engineering and improved knowledge of immune pro

42 Recent developments in genetic engineering and intravital microscopy have allow

43 Using genetic engineering and isotope labeling experiments in

44 insertion mechanisms will inform methods of genetic engineering and plant transformation.

45 characterize relapse-inducing cells, we used genetic engineering and proliferation-sensitive dyes in

46 By taking advantages of the genetic engineering and self-assembly, we propose the si

47 With the aid of genetic engineering and subsequent mutant analysis, the

48 oscience novels use selected technologies of genetic engineering and synthetic biology to create ente

49 many tools that have advanced the fields of genetic engineering and synthetic biology.

50 s glycerol in MECs and provide insights into genetic engineering and system design approaches that ca

51 r immunology and revealing new challenges in genetic engineering and target selection.

52 Advances in genetic engineering and understanding of pathogenesis ha

53 , expanding the list of animal models, using genetic engineering, and capitalizing on new miniaturize

54 dvances in the selection of optimal T cells, genetic engineering, and cell manufacturing are poised t

55 xtinction through selective breeding without genetic engineering, and fuels the topical controversy o

56 mbineering is an efficient method of in vivo genetic engineering applicable to chromosomal as well as

57 are discussed here, have indicated that the genetic engineering approach to altering floral scents h

58 A new genetic engineering approach was developed whereby varia

59 Direct, somatic, genetic engineering approaches allow for accelerated and

60 Current genetic engineering approaches almost exclusively employ

61 is a promising endogenous genetic element in genetic engineering approaches requiring spatio-temporal

62 lation in energy crops, and lead to rational genetic engineering approaches to modify lignin for impr

63 ng the quality of lignocellulosic biomass by genetic engineering approaches.

64 Receptor-specific proteins produced by genetic engineering are attractive as PET imaging agents

65 evelopment through conventional breeding and genetic engineering are reviewed.

66 of plant genetic improvement, including crop genetic engineering, are in place, and are expected to p

67 e 1976 "swine flu affair," possibilities for genetic engineering as an alternative to poultry vaccina

68 o revolutionize our view of systems biology, genetic engineering as well as disease mechanisms and mo

69 The human skin is a promising conduit for genetic engineering, as it is the largest and most acces

70 the future of plant biotechnology and plant genetic engineering, as they might help in the developme

71 Their protein subunits can be modified by genetic engineering at predetermined positions, allowing

72 Jaeger to engage with the public on GMOs and genetic engineering broadly.

73 hnology's greatest successes, with all plant genetic engineering building on the strategies of this p

74 designed nucleases (CDNs) greatly facilitate genetic engineering by generating a targeted DNA double-

75 Genetic engineering by homology-directed repair (HDR) is

76 Toward this goal, genetic engineering can be used to make protoxin express

77 Our results indicate that genetic engineering can provide a viable tool for enhanc

78 Thus, genetic engineering can selectively alter the multiple a

79 We combined yeast genetic engineering, chemical biology, and multiwaveleng

80 By combining genetic engineering, chromatin biochemistry, and single-

81 the first reports to bring the potential of genetic engineering closer to realization by improving d

82 Recent advances in plant genetic engineering could reduce biomass conversion cost

83 exploited as powerful and flexible tools for genetic engineering: Cre primarily in vivo and lambda In

84 bryonic stem cells (mESCs) are key tools for genetic engineering, development of stem cell-based ther

85 Recent genetic engineering developments need to be adapted and

86 eps by exploiting the powerful techniques of genetic engineering, directed evolution, and biomimetics

87 This work has also inspired genetic engineering efforts aimed at generating crops wi

88 Genetic engineering enables the insertion or replacement

89 tope tag in recombinant CD95L variants or by genetic engineering-enforced formation of hexamers.

90 ces the consequences of inherited mutations, genetic engineering, environmental and genetic perturbat

91 The past few decades have seen the field of genetic engineering evolve at a rapid pace, with neurosc

92 architecture, and will have implications in genetic engineering experiments, gene therapy, and under

93 Genetic engineering for complex or combined traits requi

94 lar diagnostics for nematode identification, genetic engineering for host resistance, and the elucida

95 g transport and its potential application in genetic engineering for increasing pathogen resistance a

96 use as a facile and powerful means of plant genetic engineering for scientific and agricultural appl

97 mmunotherapy, and can be further enhanced by genetic engineering for targeted immunotherapy.

98 ttes at base-level precision is transforming genetic engineering from a laborious art to an informati

99 e many classes of genomic novelties; natural genetic engineering functions are regulated and subject

100 ents; cells can target the action of natural genetic engineering functions to particular genome locat

101 the loss of homeostatic control over natural genetic engineering functions.

102 1016 1016 References 1016 Genetic engineering (GE) can be used to improve forest p

103 Thus, as T cell genetic engineering has become clinically relevant, we a

104 maging technologies with the capabilities of genetic engineering has created novel opportunities for

105 Genetic engineering has developed around technologies en

106 Genetic engineering has enabled significant, accepted in

107 Genetic engineering has enabled the development of a var

108 The term plant genetic engineering has long conveyed a highly efficient

109 Plant genetic engineering has made significant contributions t

110 Genetic engineering has made the mouse an invaluable too

111 Genetic engineering has potential to enrich diets signif

112 Mouse genetic engineering has revolutionized our understanding

113 Plant genetic engineering has, until now, relied on the incorp

114 cines in plants, especially fish vaccines by genetic engineering, has not yet been addressed.

115 Advances in genetic engineering have allowed the introduction or dep

116 ding, molecular marker assisted breeding and genetic engineering have already had, and will continue

117 Recent advances in genetic engineering have enabled the delivery of clinica

118 nthetic biology, advances in immunology, and genetic engineering have made it possible to generate hu

119 Advances in genetic engineering have overcome these limitations by i

120 ology; microbiology, immunology, and medical genetics; engineering, imaging, and synthetic chemistry;

121 become one of the most widely used tools for genetic engineering in eukaryotes.

122 -specific silencing of genes may be used for genetic engineering in mice and has possible therapeutic

123 Genetic engineering in mice enables the time-controlled

124 Using genetic engineering in mice, we have generated and chara

125 Genetic engineering in pigs holds a great promise in bio

126 ed, some of which represent the most complex genetic engineering in plants to date.

127 as the bottleneck for the next generation of genetic engineering in plants.

128 ice of the most appropriate T-SSR system for genetic engineering include that, whenever possible, the

129 ny applications in functional proteomics and genetic engineering, including codon optimization for he

130 in mammalian cells and is being applied for genetic engineering, including gene therapy.

131 In the search for renewable energy sources, genetic engineering is a promising strategy to improve p

132 tacking in trait development process through genetic engineering is becoming complex with increased n

133 Genetic engineering is being applied to eliminate or sub

134 Genetic engineering is entering a new era, where microor

135 Plant genetic engineering is routinely performed with the path

136 In the present study, chloroplast genetic engineering is used for the first time to our kn

137 r through the identification of mutations or genetic engineering, kernels with reduced levels of zein

138 rosis can be achieved in animals by germline genetic engineering, leading to hypercholesterolemia, bu

139 n-binding motif into a canonical one through genetic engineering led to a stable reddish rubredoxin.

140 Plant genetic engineering led to the production of plant-deriv

141 conifer stems through genomic selection and genetic engineering may increase resistance to bark beet

142 The rapidly improving tools of genetic engineering may make it possible to overcome the

143 (LAB) have been modified by traditional and genetic engineering methods to produce new varieties.

144 of a pre-existing template as in traditional genetic engineering methods.

145 omethionine instead of natural methionine by genetic engineering methods.

146 e T cell reactivity synthetically or through genetic engineering might thus be translated into new th

147 ntres; cells have a broad variety of natural genetic engineering (NGE) functions for transporting, di

148 An array of methods based on genetic engineering now complements older methods such a

149 Genetic engineering now enables the design of live viral

150 Here we describe the genetic engineering of a "chimeric" HPr/NPr protein, ter

151 ication in agriculture, for instance through genetic engineering of ABA receptors.

152 Genetic engineering of Ad5 capsid proteins has been empl

153 Therefore, AAV-mediated genetic engineering of adipose tissue represents a usefu

154 Acute genetic engineering of adult cardiac myocytes was used t

155 se a major barrier to DNA transformation and genetic engineering of bacterial species.

156 ocardial repair is significantly enhanced by genetic engineering of CPCs with Pim-1 kinase.

157 This is relevant for the genetic engineering of crops with economic interest that

158 From domestication and breeding to the genetic engineering of crops, plants provide food, fuel,

159 DC also participate in T cell tolerance, and genetic engineering of DC to modulate T cell immunity is

160 Genetic engineering of DC to regulate the outcome of the

161 Genetic engineering of DCs is a promising approach for t

162 The genetic engineering of EPSPS led to the introduction of

163 Recent advances in genetic engineering of ES cells have shifted the bottlen

164 Genetic engineering of hCPCs with Pim-1 enhanced repair

165 The genetic engineering of hematopoietic stem cells is the b

166 f protective MHC class II expression through genetic engineering of hematopoietic stem cells.

167 in microbes as well as genomic fragments for genetic engineering of higher eukaryotes.

168 We have also recently shown that the genetic engineering of human dendritic cells (DCs) to ex

169 These are based on genetic engineering of human genomic safe harbors combin

170 Targeted genetic engineering of human pluripotent cells is a prer

171 pplication of ZFN technology to the targeted genetic engineering of human pluripotent stem cells and

172 HEGs could be used to take the step from the genetic engineering of individuals to the genetic engine

173 microalgae points to new possible avenues of genetic engineering of lipid metabolism in this organism

174 f genome modifications, their application to genetic engineering of livestock has been slowed by tech

175 We expect that further genetic engineering of M13 viruses can allow us to coass

176 cient use of ARTs, facilitating their use in genetic engineering of macaque monkeys for basic and tra

177 gence of novel pathways in nature as well as genetic engineering of microbes for "green" manufacturin

178 l for providing the foundation necessary for genetic engineering of microorganisms for industrial use

179 Genetic engineering of model organisms and cultured cell

180 rogram population-level dynamics through the genetic engineering of multiple cooperative strains poin

181 osynthesis can refer to any strategy for the genetic engineering of natural product biosynthesis to o

182 d and quality through molecular breeding and genetic engineering of new cotton cultivars.

183 es and has potentially broad applications in genetic engineering of oleaginous crops and microorganis

184 The aECM formed through self-assembly and genetic engineering of phage can be used to understand t

185 describe a comprehensive protocol to perform genetic engineering of phage, liter-scale amplification,

186 efine a commercially viable strategy for the genetic engineering of phytate-free grain and provide in

187 Genetic engineering of plants for natural products can h

188 y of promoter sequences is necessary for the genetic engineering of plants, in basic research studies

189 describes the state of the art in multigene genetic engineering of plants.

190 etic transformation is an efficient tool for genetic engineering of plants.

191 transformation is a major technique for the genetic engineering of plants.

192 he genetic engineering of individuals to the genetic engineering of populations.

193 on to the brain drug delivery problem is the genetic engineering of recombinant fusion proteins.

194 lated tissues can help in the development of genetic engineering of resistance against this pathogen.

195 s of the glycoprotein hormone family was the genetic engineering of single chains comprised of the co

196 Recent progress in genetic engineering of spider silks and the development

197 Safe genetic engineering of stem cells, using facile methods

198 Genetic engineering of structure-designed bromodomain an

199 Twenty-five years after its inception, the genetic engineering of T cells is now a therapeutic moda

200 ty have included in vitro stimulation and/or genetic engineering of T cells, followed by adoptive tra

201 ter gene expression and involves the ex vivo genetic engineering of T lymphocytes with a reporter gen

202 Genetic engineering of the biosynthetic pathway in the p

203 iments demonstrate the potential for precise genetic engineering of the Drosophila genome with the ph

204 Rapid and stable genetic engineering of the liver would allow systematic,

205 Genetic engineering of the nonribosomal peptide syntheta

206 primate, if these can be resolved by further genetic engineering of the organ-source pigs, a pig live

207 er nuclease (ZFN) technology has enabled the genetic engineering of the rat genome.

208 fied which can assist in rapid and efficient genetic engineering of these bacteria in the future.

209 r activity, demonstrating the feasibility of genetic engineering of these cells.

210 Here we report rapid and targeted genetic engineering of this parasite using zinc-finger n

211 Genetic engineering of Tyt1 variants devoid of cysteines

212 pora tropica by mutagenesis to allow for the genetic engineering of unnatural derivatives of the pote

213 ces making them potentially suitable for the genetic engineering of wild-type populations of animals

214 Chloroplast genetic engineering offers several unique advantages, in

215 In addition to genetic engineering, oligo-induced mutagenesis may have

216 obial catalysts via culturing approaches and genetic engineering or adaptive evolution, make this pla

217 escribed methods, GlycoFRET does not require genetic engineering or antibodies to label receptors.

218 Targeting these factors via genetic engineering or breeding approaches can allow dyn

219 a novel strategy for extending shelf life by genetic engineering or conventional breeding.

220 oninsect species, would be a good target for genetic engineering or novel insecticides.

221 Chloroplast genetic engineering overcomes concerns of gene containme

222 h gene sequencing, comparative genomics, and genetic engineering, precisely establish which genes are

223 a technique that could prove useful for many genetic engineering procedures.

224 ndicate that if a gene or trait is safe, the genetic engineering process itself presents little poten

225 and provided novel insights into the natural genetic engineering processes involved in evolution.

226 ation system is a useful tool in a number of genetic engineering processes.

227 Genetic engineering projects often require control over

228 Large genetic engineering projects require more cistrons and c

229 ected to facilitate various kinds of complex genetic engineering projects that require precise in-fra

230 d on average, which could hinder large-scale genetic engineering projects.

231 nderstanding algal metabolism and developing genetic engineering protocols.

232 Genetic engineering provides an alternative approach to

233 Genetic engineering provides an ingenious method of atte

234 Many applications of genetic engineering require transformation with multiple

235 enoids in plastids through plant breeding or genetic engineering requires an understanding of the lim

236 e ease with which they can be modified using genetic engineering, scFv's have significant advantages

237 Improvements in plant photosynthesis by genetic engineering show considerable potential towards

238 optimise fermentation conditions and improve genetic engineering strategies among others.

239 implications of concentration robustness in genetic engineering strategies and medical applications.

240 us cell wall will help to steer breeding and genetic engineering strategies for the development of su

241 to N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this n

242 In this study, we demonstrate genetic engineering strategies to overcome these shortco

243 to overcome the limitations of conventional genetic engineering strategies.

244 e importance of macrophage mitoOS, we used a genetic engineering strategy in which the OS suppressor

245 and efficient gene editing than all previous genetic engineering systems.

246 years as a result of recent improvements in genetic engineering techniques and the availability of m

247 lly, we highlight the latest developments in genetic engineering techniques and their possible applic

248 Advances in tissue and genetic engineering techniques are expected to improve t

249 s, have now been used to manipulate, through genetic engineering techniques, the mix of volatiles emi

250 Genetic engineering technologies can be used both to cre

251 genomics and genetics combined with improved genetic engineering technologies offer a wide range of p

252 A limited number of analogs produced by genetic engineering technologies show reduced cytotoxici

253 h high-throughput genotyping, sequencing and genetic engineering technologies, and information reposi

254 iology underlying ripening and the advent of genetic engineering technologies, researchers have pursu

255 e major limitations to extending chloroplast genetic engineering technology to useful crops.

256 NOD ES cells are unsuitable for the complex genetic engineering that is required to improve human he

257 Staudinger ligation, NCA polymerisation, and genetic engineering, that have been used to great effect

258 ough Agrobacterium can be popularly used for genetic engineering, the influence of aboveground insect

259 The advent of genetic engineering-the ability to edit and insert DNA i

260 escribe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitut

261 tumours, and aided by genomic sequencing and genetic engineering, there is new interest in the use of

262 to tune the preferred pH of bioactuators by genetic engineering, these biohybrid microsystems could

263 g both sophisticated chemistry and extensive genetic engineering, this technology provides a convenie

264 We examined the feasibility of utilizing genetic engineering to circumvent the restricted capacit

265 plant detoxification systems and the use of genetic engineering to combine bacterial explosives-deto

266 approaches must be adopted involving use of genetic engineering to enhance resistance to cell death

267 ave inspired novel approaches based on using genetic engineering to manipulate vector populations and

268 ity of novel fatty acids in oilseeds through genetic engineering to meet the increasing demands of th

269 nsferred cardiac progenitor cells (CPCs) via genetic engineering to overexpress Pim-1, a cardioprotec

270 This work lays the foundations for using genetic engineering to produce cellulose-based materials

271 to the environment and without the need for genetic engineering to safeguard feed and food supply fo

272 Our results are the first example of genetic engineering to significantly alter the polymeriz

273 approaches, reprogramming patient cells and genetic engineering, to generate human induced pluripote

274 We present here the first to our knowledge genetic engineering tool that will efficiently allow for

275 It constitutes contribution to the genetic engineering toolkit of photosynthetic microorgan

276 The genetic engineering tools and resources established in t

277 y be applied to many other species for which genetic engineering tools are needed.

278 rganism that until recently lacked efficient genetic engineering tools.

279 he feasibility of protecting animals through genetic engineering, transgenic cows secreting lysostaph

280 o biotechnology--genetic modification, GMOs, genetic engineering, transgenic, and all the rest--has b

281 RATIONALE: Targeted genetic engineering using programmable nucleases such as

282 Genetic engineering was undertaken to study the strength

283 In the study by Gorelik and colleagues (1), genetic engineering was used to overcome this limitation

284 This method, which does not require genetic engineering, was used to produce an anti-CD22 an

285 Using genetic engineering, we establish a link between the int

286 or structural modification of meridamycin by genetic engineering, we have cloned and completely seque

287 By genetic engineering, we inverted its relative levels of

288 live-cell single-molecule tracking (SMT) and genetic engineering, we reveal that H3K27me3 contributes

289 nation of genomics, bioinformatics and mouse genetic engineering, we scanned approximately 700 kb of

290 single-molecule superresolution imaging and genetic engineering, we study in living Escherichia coli

291 regulatory strategy by chemical adjuvants or genetic engineering, which is restricted by limited syne

292 rative genomics, plant breeding, and plastid genetic engineering, while shedding further light on the

293 ending problems, significant improvements in genetic engineering will be needed to complement breedin

294 tated lignin recovery, and this coupled with genetic engineering will enable new uses for this biopol

295 sion on cardiac contractility, we used acute genetic engineering with a recombinant adenoviral vector

296 tion in this work was the coupling of direct genetic engineering with a refolding approach to produce

297 s "super" immune cells, for example, through genetic engineering with chimeric antigen receptors.This

298 Genetic engineering with EPO may be a viable approach fo

299 These developments promise genetic engineering with unprecedented levels of design

300 nted-by either chemical functionalization or genetic engineering-with hundreds to thousands of functi