ライフサイエンスコーパス: genetic screen

1 IpaB mutants using random mutagenesis and a genetic screen.

2 were identified using a transposon-mediated genetic screen.

3 iptional gene silencing (TGS) from a forward genetic screen.

4 Under Glass (Hug), recovered from a forward genetic screen.

5 ding protein-associated factor 11 (TAF11) by genetic screen.

6 oup of immotile mutants in the 1996 Tubingen genetic screen.

7 omplementary information in loss-of-function genetic screens.

8 redundant and, thus, undetectable by forward genetic screens.

9 gene interaction studies and high-throughput genetic screens.

10 and active zones, and perform rapid forward genetic screens.

11 wing for robust high-throughput chemical and genetic screens.

12 rom genome visualization and manipulation to genetic screens.

13 cute myeloid leukaemia cells in two distinct genetic screens.

14 ping new mutations generated through forward genetics screens.

15 particularly with the application of pooled genetic screening.

16 ferentiation potential and applicability for genetic screening.

17 immune response than pretreatment biopsy or genetic screening.

18 ese cells as a platform for loss-of-function genetic screening.

19 l in next generation chips for bioassays and genetic screening.

20 rmination of the nonclinical implications of genetic screening.

21 Through a genetic screen aimed to identify genetic modifiers of TD

22 Despite numerous genetic screens aimed at identifying genes involved in R

23 atmosphere, have been largely discovered in genetic screens aimed to isolate mutants that are unviab

24 Our genetic screen also reveals that ch-TOG maintains a dyna

25 ese motifs from both human disease and yeast genetic screens alter the physical interaction with Prp5

26 e how ECO1 promotes cohesion, we conducted a genetic screen and identified a cohesion activator mutat

27 implementation of a high-throughput chemical genetic screen and the identification of a small-molecul

28 mutagenesis expands the toolbox for forward genetic screening and also provides direct evidence that

29 undant collection that is suitable for rapid genetic screening and gene discovery.

30 We use results of forward genetic screening and genetic analysis in our new model

31 Through a combination of CRISPR-Cas9-based genetic screening and metabolomic analyses, we show that

32 ated by other experiments such as functional genetic screens and expression profiling.

33 fects to enable more effective and efficient genetic screens and genome engineering.

34 ice many laboratories have conducted forward genetic screens and identified thousands of candidate dr

35 reated new opportunities for high-throughput genetic screens and multiplexing targets to manipulate c

36 accuracy of cancer gene discovery in forward genetic screens and provide initial insight into the bio

37 We first determine, through unbiased genetic screens and systematic genetic analyses, that au

38 n for other uses, such as synthetic biology, genetic screens, and CRISPR-Cas9.

39 otypic patterns may increase productivity in genetic screens, and facilitate the study of genetic var

40 Here we use a pooled in vivo genetic screening approach using CRISPR-Cas9 genome edit

41 Using a genetic screening approach, we identify the ubiquitin-sp

42 CRISPR-Cas9-based genetic screens are a powerful new tool in biology.

43 Genetic screens are powerful tools for identifying genes

44 Forward genetic screens are powerful tools for the discovery and

45 Forward genetic screens are powerful tools for the unbiased disc

46 Here, we designed a genetic screen around E. coli that identified high-affin

47 A genetic screen based on an engineered synthetic reporter

48 We used a combination of forward genetic screening based on a Proline Dehydrogenase1 (PDH

49 We adapted a genetic screen, based on Ura3 mistargeting from mitochon

50 s) provide new possibilities for large-scale genetic screens because they bear only one copy of each

51 CRISPulator facilitates the design of pooled genetic screens by enabling the exploration of a large s

52 Here we report on a genome-wide genetic screen, coupled with whole-genome sequencing, th

53 mutations in Nardilysin (dNrd1) in a forward genetic screen designed to isolate genes whose loss caus

54 In an unbiased forward genetic screen designed to isolate mutations that cause

55 also discuss the application of genome-wide genetic screening efforts to gain insight into synthetic

56 We have performed an unbiased genetic screen, finding that smg-1 mutations confer stro

57 Here, using unbiased forward genetic screens, followed by analysis of reporters and e

58 .1 mutant, which was identified in a forward genetic screen for Arabidopsis (Arabidopsis thaliana) mu

59 A genetic screen for Arabidopsis mutants affecting tempera

60 In a genetic screen for C. elegans mutants with defects in do

61 In a genetic screen for cellular factors involved in preventi

62 d trypanosome VSG exclusion-1 (VEX1) using a genetic screen for defects in telomere-exclusive express

63 A genetic screen for defects in the organization of interm

64 A zebrafish genetic screen for determinants of susceptibility to Myc

65 We identified mutations in Rab8 in a genetic screen for enhancement of an FTD phenotype assoc

66 In a genetic screen for enhancers of sic-1, we isolated a los

67 mb pathway in plants, we performed a forward genetic screen for enhancers of the like heterochromatin

68 CDC55 was identified in a genetic screen for monopolins performed by isolating sup

69 A genetic screen for mutants defective for GAL gene memory

70 By performing an unbiased genetic screen for mutants that impair the somnogenic ef

71 In a genetic screen for mutations that affect Drosophila eye

72 In a forward genetic screen for mutations that block PHP we identifie

73 To address this question, we conducted a genetic screen for mutations that differentially affecte

74 ing and tissue repair, we have carried out a genetic screen for mutations that impair regeneration in

75 From a forward genetic screen for myelination defects in zebrafish, we

76 atopoietic stem cells to carry out a forward genetic screen for Plasmodium falciparum host determinan

77 is thaliana mutant smd1b was identified in a genetic screen for PTGS deficiency, revealing the involv

78 In a forward genetic screen for regulators of C. elegans PKD-2 ciliar

79 n minus-end directed microtubule motor, in a genetic screen for regulators of EGFR signaling.

80 In a chemical genetic screen for small molecules that dampened the inh

81 on (lf) mutants of hrpu-2 were isolated in a genetic screen for suppressors of a sluggish phenotype c

82 By deploying a genetic screen for suppressors of cell death triggered b

83 rt machinery component Tic40, we performed a genetic screen for suppressors of chlorotic tic40 knocko

84 In a genetic screen for suppressors of reduced neurotransmitt

85 Here, we report the results of a forward genetic screen for suppressors of ref4-3.

86 In this study, we developed a forward genetic screen for the identification of host factors re

87 Genetic screening for a germline mutation at the RET gen

88 arcoded mutants unlocks the power of reverse genetic screening for a malaria parasite and will enable

89 e lhr1 mutant was isolated through a forward genetic screening for altered expression of the lucifera

90 se of hematopoietic stem cells to facilitate genetic screening for malaria host factors.

91 Our genetic screening for mutations that resist CLE peptide

92 approaches have hindered systematic forward genetic screening for NMD factors in human cells.

93 Fireworks) that enables CRISPR-based forward genetic screening for NMD pathway defects in human cells

94 challenges faced by families as a result of genetic screening for SADS to enable equitable access to

95 s and demonstrates the promise of functional genetic screens for dissecting therapeutically relevant

96 Here, we describe two independent genetic screens for rsc suppressors that yielded mutatio

97 A chemical genetic screen found that the compound lycorine promotes

98 ottleneck previously associated with forward genetic screens has significantly advanced the use of ge

99 Large-scale forward genetic screens have been instrumental for identifying g

100 cells grow in a highly polarized manner, and genetic screens have identified many protein kinases, in

101 Functional genetic screens have recently revealed that cancer cell

102 Recently, genetic screens have suggested that colicin N (ColN), wh

103 Genetic screens help infer gene function in mammalian ce

104 Using genome-wide haploid genetic screens, here we identify the lipid-modifying en

105 A forward genetic screen identified mutations in met-1, which enco

106 A genome-wide haploid genetic screen identified the transmembrane protein neur

107 and CELLULOSE SYNTHASE 3 (CESA3), a forward genetic screen identified two independent defective gene

108 In addition to known oncogenes, the genetic screen identifies genes that contribute to cellu

109 The Fireworks genetic screen identifies multiple known NMD factors and

110 Genetic screening identifies the atypical tetraspanin TM

111 tify such mechanisms, we conducted a forward genetic screen in a C. elegans model of polyglutamine ag

112 We conducted a synthetic chemical-genetic screen in a kns1Delta mck1Delta strain and ident

113 Using a forward genetic screen in a tumor-derived human cell line, we id

114 we conducted a genome-wide loss-of-function genetic screen in an isogenic pair of human colorectal c

115 From a mutant snc1-enhancing (MUSE) forward genetic screen in Arabidopsis targeted to identify negat

116 B1 (HLB1) protein isolated through a forward-genetic screen in Arabidopsis thaliana for mutants with

117 We have developed a genetic screen in Aspergillus nidulans for negative regu

118 Through a genetic screen in BRAF-mutant tumor cells, we show that

119 levels of DA clearance, we pursued a forward genetic screen in Caenorhabditis elegans based on the ph

120 In this study, we utilize a forward genetic screen in Caenorhabditis elegans to identify a g

121 Using a forward genetic screen in Drosophila, we identified an E3 ligase

122 Here, through a genome-wide genetic screen in Drosophila, we uncover a highly comple

123 Here, we performed a genetic screen in Escherichia coli on the LCFA, oleate,

124 we report findings from a recessive forward genetic screen in fetal mice, showing that cilia and cil

125 ne doxorubicin based on the use of a forward genetic screen in haploid human cells, followed by a rig

126 Here, we used a loss-of-function genetic screen in human haploid KBM7 cells to discover t

127 We used a novel genetic screen in larval zebrafish to reveal the molecul

128 Here we report a large-scale genetic screen in mice employing a phenotype-driven disc

129 e used an N-ethyl-N-nitrosurea-based forward genetic screen in mice to identify new genes and alleles

130 leeping Beauty (SB) transposon-based forward genetic screen in mice with and without somatic loss of

131 biosynthesis in mycobacteria, we executed a genetic screen in Mycobacterium smegmatis for biotin aux

132 Here, we use an unbiased genome-wide genetic screen in near-haploid human cells to uncover ce

133 A nonlethal forward genetic screen in near-haploid KBM7 cells identified the

134 Here, through a forward genetic screen in planta, we identify a conserved amino

135 We performed a genetic screen in the ag-11 background to isolate mutati

136 We used a genetic screen in the developing Drosophila melanogaster

137 rstood, particularly in vivo Using a forward genetic screen in the nematode Caenorhabditis elegans, w

138 performed a genome-wide unbiased functional genetic screen in TP53 mutant near-haploid KBM-7 cells u

139 ngiogenesis, we performed a morpholino-based genetic screen in zebrafish and identified Cavin-2, a me

140 By performing a forward genetic screen in zebrafish we isolated no food for thou

141 te vertebrate sleep, we recently performed a genetic screen in zebrafish, and here we report the iden

142 Through a forward genetic screen in zebrafish, we discovered that mutation

143 Here, using a forward genetic screen in zebrafish, we find that the autism-ass

144 From a forward genetic screen in zebrafish, we identify the transcripti

145 we performed a large scale loss-of-function genetic screen in ZR-75-1 luminal breast cancer cells to

146 These findings may help direct genetic screening in a busy neurology outpatient setting

147 Through genetic screening in C. elegans, we uncover two metformi

148 We used the power of genetic screening in human cells and found that RVFV uti

149 oxicity mechanism, made possible by unbiased genetic screening in human cells, suggests that the sele

150 e address this need by using high-throughput genetic screening in yeast to select variants of the iro

151 omedical researchers to conduct CRISPR-based genetic screens in a pooled format.

152 ostasis and lipid metabolism.High-throughput genetic screens in animals could benefit from an easy wa

153 Forward genetic screens in Drosophila melanogaster for modifiers

154 specifically highlighting the use of forward genetic screens in human haploid cells.

155 demonstrate the utility of two-step forward genetic screens in mice, and offer an invaluable tool to

156 Sleeping Beauty DNA transposon-based forward genetic screens in mice.

157 In vivo genetic screens in tumour models can identify new immuno

158 We perform genetic screens in two bacterial species using three che

159 Transposon-mediated forward genetics screening in mice has emerged as a powerful too

160 Forward genetics screens in mice have facilitated the identifica

161 A forward genetic screen is one of the best methods for revealing

162 Haploid genetic screening is a powerful tool to reveal factors i

163 It is unknown whether genetic screening is indicated in the general population

164 The forward genetic screen led to the identification of a novel HIST

165 Hematopoietic stem cell-based forward genetic screens may be valuable for the identification o

166 est that other essential genes identified by genetic screens may turn out to be components of selfish

167 Here, we use a combination of genetic screening, MD simulations, and biochemical and m

168 Here we present a genetic screening method using photo-highlighting for ca

169 a parasites is hampered by a lack of reverse genetic screening methods.

170 High-throughput datasets such as genetic screens, mRNA expression assays and global phosp

171 Here, we present the first genome-wide genetic screen of an apicomplexan.

172 In an independent functional genetic screen of Drosophila cardiac nephrocytes, which

173 ronal orphan GPCR called Gpr45, in a forward genetic screen of mutant mice generated by piggyBac inse

174 Genetic screening of 133 unaffected Hungarian Vizslas re

175 Finally, genetic screening of 44 patients revealed >/=2 ABCA4 mut

176 Genetic screening of a further 367 isolated dystonia sub

177 We recommend that genetic screening of aHUS includes analysis of CFH and C

178 Following genetic screening of Parkinson's disease patients and he

179 Genetic screening of patients diagnosed with macular dys

180 2012, the authors have included FLNC in the genetic screening of patients with inherited cardiomyopa

181 To maximize clinical benefits of genetic screening of patients with nephrotic syndrome (N

182 ses a MELAS-like phenotype, and suggests the genetic screening of the MRM2 gene in patients with a m.

183 Genetic screens of 19 million C. elegans mutants reveal

184 Genetic screens of an unprecedented scale have recently

185 encing factor was identified using a forward genetic screen on a reporter line that harbors a LUCIFER

186 plexed CRISPR/Cas9 can be used for recessive genetic screening or high-throughput cancer gene validat

187 ed for disease modeling, small molecules and genetic screens, or applied to human pluripotent stem ce

188 Here, we describe a forward genetic screening paradigm exploiting CRISPR-mediated ge

189 To address this gap, we analyzed large-scale genetic screens performed in human cell lines using eith

190 Classical genetic screens performed over the past half century ide

191 In our genetic screening, Pink1 and Park genes were identified

192 pan assays limit their usefulness as a broad genetic screening platform for research on aging.

193 gm with significant potential for developing genetic screening platforms in mammalian cells.

194 Genetic screening programs in unselected individuals wit

195 /Cas9-mediated screens together with haploid genetic screens provide a powerful addition to the forwa

196 Our re-analysis of a previous genetic screening result in Caenorhabditis elegans shows

197 Pathway-oriented genetic screens reveal that CRC cells escape from EGFR b

198 A genetic screen revealed an unexpected interaction of MYO

199 A genetic screen revealed that an ambient-exposed region c

200 Our genetic screening revealed varying mutation frequencies

201 Surprisingly, genetic screening reveals that yeast FTase can modify se

202 This forward genetic screening scheme is useful and applicable to any

203 esults demonstrate the power of our chemical-genetic screening strategies for pinpointing the physiol

204 hat CRISPR can be used as a powerful reverse genetic screening strategy in vivo in a vertebrate syste

205 Here we describe a genetic screening strategy to isolate fertilization muta

206 should be adopted in future mechanistic and genetic screening studies.

207 Forward genetic screening suggests that multiple receptors are i

208 Using a yeast genetic screening system, we identify Lhx3 point mutants

209 In a genetic screen targeted to identify genes regulating sto

210 g engineered transposons is a potent forward genetic screening technique used to identify cancer gene

211 Here we report a genetic screen that highlighted H2B deubiquitylation and

212 in cells and intestinal organoids, and in a genetic screen that identified a role for NEDD4L in regu

213 describe the use of the library in a forward genetic screen that identified InaC as a bacterial facto

214 is a story of a simple yet insightful yeast genetic screen that revealed the inner circuitry of one

215 Using the FSCs as a model, we performed a genetic screen through a collection of 126 mutants in es

216 We developed a genetic screen to determine the pathogenicity of these v

217 Previously, we used a positive genetic screen to identify an Arabidopsis mutant, cli186

218 d on a two-step Sleeping Beauty (SB) forward genetic screen to identify and validate new tumor suppre

219 We used a genetic screen to identify factors critical to the Ire1-

220 We performed a genetic screen to identify factors responsible for gluco

221 phila homolog of SLC25A39 and SLC25A40, in a genetic screen to identify genes involved in neuronal fu

222 We used a CRISPR-based genetic screen to identify genes whose loss sensitizes h

223 n this study, we performed a high-throughput genetic screen to identify kinases that enable tumor for

224 We have developed a genetic screen to identify mutants defective in placemen

225 (Arabidopsis thaliana) root tip, allowing a genetic screen to identify mutants impaired in RDR6-depe

226 Using a genetic screen to identify novel regulators of fimE and

227 Here we use an unbiased genetic screen to identify proteins essential for AAV se

228 Here we use a forward-genetic screen to identify rbm25-1 and rbm25-2, two Arab

229 We used a reverse genetic screen to identify Recognition of XopQ 1 (Roq1),

230 insight into this process, we used a forward genetic screen to identify the regulatory components gov

231 e Drosophila leg and used a powerful forward genetic screen to identify three novel suppressor genes.

232 gene of unknown function that was found in a genetic screen to interact with the mce4 cholesterol upt

233 We describe here a forward genetic screen to investigate the biogenesis, mode of ac

234 egress through CDPKs, we performed a forward genetic screen to isolate gain-of-function mutants from

235 In a genetic screen to isolate loss-of-function mutations in

236 rabidopsis thaliana), we conducted a forward genetic screen to isolate mutants that fail to enter dor

237 Here we used a pathway-activating genetic screen to nominate MCL-1 and BCL-XL as potential

238 onditional Cas9/CRISPR system to carry out a genetic screen to verify if these factors were involved

239 Our studies demonstrate the power of genetic screening to discover cancer drivers that are di

240 s in this group highlights the importance of genetic screening to identify abnormalities that may be

241 source allows single-target or combinatorial genetic screens to be carried out at scale in a multiple

242 The application of forward genetic screens to cultured human cells represents a pow

243 they can be used for high-throughput reverse genetic screens to help functionally annotate the Ae. ae

244 ying mechanism of this process, we performed genetic screens to identify components of the sorting ma

245 Here we describe a panel of genetic screens to identify genes required for replicati

246 We can perform genetic screens to identify novel genes involved in spec

247 We use the Haplobank in reverse genetic screens to investigate the temporal resolution o

248 otein-protein interaction (PPI) networks and genetic screens to predict the 'signs' of interactions (

249 shed genetic and developmental model used in genetic screens to uncover genes necessary for early dev

250 nity, we designed and carried out a chemical genetics screen to search for small molecules that can a

251 As CRISPR-Cas is a relatively new genetic screening tool, it is important to assess its fu

252 is model was used in a large-scale, unbiased genetic screen, ultimately leading to the identification

253 Here we show results from a genetic screen using an in vivo invasion model via knock

254 chromatin function, we conducted a candidate genetic screen using RNAi lines targeting known PEV modi

255 Here, we performed a chemical genetic screen using zebrafish and discovered that prost

256 Following genetic screening using a shade-responsive luciferase re

257 hat improves the efficiency of combinatorial genetic screening using an effective strategy for clonin

258 Combinatorial genetic screening using CRISPR-Cas9 is a useful approach

259 Genetic screening using flagellin mutants of L. pneumoph

260 Genetic screening using random transposon insertions has

261 Large-scale genetic screens using CRISPR/Cas9 technology have emerge

262 terspaced palindromic repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries

263 Forward genetic screens using Sleeping Beauty (SB)-mobilized T2/

264 A forward genetic screen utilizing Sleeping Beauty mutagenesis in

265 of genome-wide CRISPR/Cas9-mediated forward genetic screens versus gene-trap mutagenesis screens in

266 derstanding of these structures, a candidate genetic screen was employed to identify regulators of st

267 In parallel experiments, a genetic screen was used to identify mutations in rgg2 of

268 The yield of genetic screening was low (14%), despite familial diseas

269 On the basis of a directed genetic screen we find that vision plays a key role in c

270 Through a forward genetic screen we found pals-22, a gene of previously un

271 Through forward genetic screens we identified PKL, a gene required for d

272 In a genetic screen, we found a zebrafish strain in which mit

273 Using a genetic screen, we found that CALCINEURIN B-LIKE INTERAC

274 Using a fluorescence-imaging-based genetic screen, we found that cngc14 mutants exhibit a c

275 Based on an unbiased genetic screen, we found that partial genetic and pharma

276 Using a forward genetic screen, we identified an allelic series of GmSHM

277 In a genetic screen, we identified Dscam4 as another regulato

278 h a genome-wide CRISPR-Cas9-mediated forward genetic screen, we identified MORC2 as an essential gene

279 Using a genetic screen, we identified the DNA-methylation machin

280 Using a new genetic screen, we identify a nuclear mutant that is req

281 Through a forward genetic screen, we identify gain-of-function mutations i

282 Through a forward-genetic screen, we isolated tara as a novel sleep gene a

283 Here by performing a genetic screen, we show that AphB represses ROS resistan

284 Here, by using a forward genetic screen, we show that the mycobacterial GatCAB en

285 axonal trafficking and performing a forward genetic screen, we showed that the microtubule cytoskele

286 Through a genetic screen, we uncovered an unanticipated link betwe

287 Using unbiased genetic screens, we delineate an IL-17 signalling pathwa

288 tegrating parallel cell-based and Drosophila genetic screens, we discovered that tau levels are regul

289 To illustrate the power of such haploid genetic screens, we highlight the discovery of the lysos

290 In forward genetic screens, we isolated a novel Caenorhabditis eleg

291 Through genetic screens, we unexpectedly find that mutant HRAS,

292 nding protein Vfa1 was identified in a yeast genetic screen, where overexpression of Vfa1 caused defe

293 pate RNA-seq analyses of tumors from forward genetic screens will become an efficient tool to identif

294 We performed a genetic screen with ag-10 plants, which exhibit a weak f

295 A forward genetic screen with ethyl-N-nitrosourea-mutagenized mice

296 esults also pave the way for high-throughput genetic screening with CRISPR/Cas.

297 We tested the hypothesis by performing three genetic screens with chemical mutagenesis in the social

298 This resource for unbiased forward-genetic screens with mapping-by-sequencing ('forward gen

299 tor receptor-overexpressing mice and forward genetic screens with Sleeping Beauty transposons implica

300 Our previous genetic screen yielded malbec (mlb(bw306)), a zebrafish