ライフサイエンスコーパス: genetic selection

1 f protein engineering that uses the power of genetic selection.

2 d biosensors and to engineer enzymes through genetic selection.

3 genes using unbiased genome-wide functional genetic selection.

4 cts positive clones by positive and negative genetic selection.

5 ring infection were recently identified in a genetic selection.

6 00)-Arg(712) by using random mutagenesis and genetic selection.

7 X inactivation but the dominant mechanism is genetic selection.

8 ng a telomeric linear plasmid and a two-step genetic selection.

9 f Na+ tolerance to yeast, can be isolated by genetic selection.

10 herefore subjected to random mutagenesis and genetic selection.

11 ence-activated cell sorter (FACS) to perform genetic selection.

12 h affinity and specificity by direct in vivo genetic selection.

13 ents between 1N and 3N without involving any genetic selection.

14 ne approaches to search for evidence of such genetic selection.

15 ype levels by random mutagenesis of purF and genetic selection.

16 unction as logic gates were isolated by dual genetic selection.

17 ied site saturation strategy with functional genetic selection.

18 dered variants of dihydrofolate reductase by genetic selection.

19 ne using ribosome binding site libraries and genetic selection.

20 or by using RNAi in phenotype-based, forward genetic selections.

21 lly applicable to the analysis of a range of genetic selections.

22 ty of Red-mediated recombination in targeted genetic selections.

23 dified yeast two-hybrid system which enables genetic selection against a specific protein/protein int

24 erated the normally slow response to relaxed genetic selection against deafness that began in many We

25 is required to be paired, we observe strong genetic selection against viruses that contain a deletio

26 ge libraries, coupled with the benefits of a genetic selection, allowed us to identify rare, uniquely

27 Using this observation as a basis for genetic selection, an extragenic suppressor of Tus-media

28 ion strategies have traditionally focused on genetic selection and cost-effective ration formulation

29 rol initiation, respectively, we developed a genetic selection and identified seqA, datA, dnaN and hd

30 Here we describe a combined genetic selection and screen that allows the rapid evolu

31 Previously, extensive genetic selection and screening approaches were employed

32 Genetic selection and site-directed mutagenesis experime

33 termination, were recovered in a powerful F1 genetic selection and used to investigate how sc was rec

34 In this study, we employed genetic selections and experimental evolution to identif

35 plant Arabidopsis thaliana were isolated by genetic selections and fluorescence screens.

36 individuals who are interested in population genetics, selection and evolution of polymorphic human g

37 ii) genes within de novo CNVs under negative genetic selection, and (iii) genes identified by clinica

38 uctase (VKORc1) as a reporter, we describe a genetic selection approach allowing the isolation of Esc

39 We developed a new genetic selection approach to screen for mutations that

40 Genetic selections are available for each behavior.

41 l aminoacyl-tRNA synthetase is generated and genetic selections are performed on the library in Sacch

42 se explanations is correct, the evidence for genetic selection at the EPAS1 locus from the GWADS stud

43 A genetic selection based on a UAS2-dependent ADH2 reporte

44 clease (RNase A) and human angiogenin, and a genetic selection based on the intrinsic cytotoxicity of

45 We used a genetic selection based on the yeast two-hybrid assay to

46 Degenerate (doped) in vitro genetic selection, based on a pool of randomized 39-mers

47 e- mediated exchange of DNA to systems where genetic selection can be applied.

48 Nearly all positive clones from the genetic selection carried mutations at lysine 155 in tra

49 of a particular residue as revealed by this genetic selection correlated with its sequence conservat

50 Here, we present results of a genetic selection designed to identify additional compon

51 Here, we present results from a genetic selection designed to identify mutants that miss

52 In a genetic selection designed to isolate Escherichia coli m

53 Using a genetic selection designed to isolate PtdIns(3)P-binding

54 We have implemented a yeast genetic selection developed previously by our laboratory

55 breeding values was shown to have a greater genetic selection differential and reduced future diseas

56 Genetic selection experiments in a glucokinase-deficient

57 Genetic selection experiments suggested that such sequen

58 We report the results of genetic selection experiments that identify nine genes f

59 In agreement with genetic selection experiments, the affinity of triclosan

60 ives of bacteriophage P22 that enable direct genetic selection for a specific RNA-protein interaction

61 unbalanced body conformation due to intense genetic selection for additional breast muscle (pectoral

62 Moreover, the effects were modified by genetic selection for body weight, which altered the qua

63 In a genetic selection for budding yeast mutants that were de

64 further dissection of phosphate signaling by genetic selection for constitutive phosphate starvation

65 by profiling Drosophila strains subjected to genetic selection for differences in aggressiveness.

66 pproaches, including saturation mutagenesis, genetic selection for DNA-binding mutants, photo cross-l

67 e DNA binding by TBP in vivo, we performed a genetic selection for extragenic suppressors of a yeast

68 rofitability (p<0.01), but an improvement in genetic selection for fertility (p<0.01) and overall cow

69 hically circumscribed population rather than genetic selection for heterosexual transmission.

70 Short Sleep lines of mice were developed by genetic selection for high or low alcohol sensitivity.

71 Using a genetic selection for increased frameshifting in a repor

72 The first tier involves a genetic selection for intracellular protein stability th

73 lating MAL gene expression, we carried out a genetic selection for MAL constitutive mutants.

74 A genetic selection for multicopy suppressors of a framesh

75 that are important for ligand specificity, a genetic selection for mutants of the inosine-guanosine-s

76 A genetic selection for mutants that activate this pathway

77 ptional regulation of PHO5, we carried out a genetic selection for mutants that express PHO5 constitu

78 ing domain barrier components, we designed a genetic selection for mutants with reduced negative supe

79 We utilized a genetic selection for mutations that affect the regulati

80 UR1 and BUR2 were previously identified by a genetic selection for mutations that increase transcript

81 w phosphatase, PTP3, have been isolated in a genetic selection for negative regulators of an osmotic

82 ) substitutions were previously isolated via genetic selection for photosynthetic ability at the 35 d

83 To this end, we applied a recently developed genetic selection for protein folding and solubility bas

84 In this study, a genetic selection for second-site mutations that could p

85 A bacterial genetic selection for second-site suppressors using an a

86 lling the postharvest storage atmosphere and genetic selection for slow or late ripening varieties.

87 Therefore, we developed a genetic selection for splicing-dependent kanamycin resis

88 ifferential behavior provided the basis of a genetic selection for STE5 gain-of-function mutations.

89 Genetic selection for suppressors of a polyadenylation d

90 Genetic selection for suppressors of pap1-1 yielded two

91 in phospholipid biosynthesis, we performed a genetic selection for suppressors of the inositol auxotr

92 Through a genetic selection for T. gondii mutants resistant to the

93 Genetic selection for the ability to repress translation

94 However, harnessing the power of genetic selection for the detection of specific, nonendo

95 Although genetic selection for thermoregulatory adaptation is fre

96 Using a genetic selection for tmRNA activity in Escherichia coli

97 Through forward genetic selection for variants capable of virus spread,

98 esigned to bind heme or CO, nor subjected to genetic selections for heme or CO binding.

99 ific disciplines of environmental design and genetic selection, however understanding the ecological

100 rent issue of the Journal do not support the genetic selection hypothesis.

101 In this study, genetic selection identified extein sequences with Ser+1

102 onucleotide formyltransferase (PurN) and, by genetic selection, identified PurN heterodimers capable

103 onal randomization of the loop, coupled with genetic selection in a glucokinase-deficient bacterium,

104 Thus, genetic selection in cancer cells can be used as a tool

105 igand, suggesting that it is possible to use genetic selection in E. coli to discover synthetic ribos

106 m sequence mutagenesis coupled with positive genetic selection in E. coli yields large numbers of fun

107 Using a genetic selection in Escherichia coli, we identified add

108 Here, we used a single round of genetic selection in mouse cells to isolate chemically s

109 Genetic selection in Saccharomyces cerevisiae for gain o

110 es in the cell of origin dramatically affect genetic selection in tumors.

111 random saturation mutagenesis coupled with a genetic selection in yeast to determine the relative imp

112 Thus, this approach, which is based on genetic selection in yeast, is safe, economic, and a rel

113 genes that play a role in this process using genetic selections in conventional cell culture systems.

114 Mutational analysis and in vitro genetic selection indicate that U2AF35 has a sequence-sp

115 When carried out in the context of a genetic selection, interacting pairs can be rapidly isol

116 ents and omit potential gains by integrating genetic selection into existing breeding programs.

117 molecular evolution to proteins for which no genetic selection is available.

118 In the practice of microbial genetics, selection is used to detect and enumerate pre-

119 Separate genetic selections isolated two AsiA mutants (S22F and Q

120 Since Top7 was created in the absence of genetic selection, it provides a rare opportunity to inv

121 In this study a genetic selection led to the identification of a bacteri

122 The fact that genetic selection maintains similar molecular interactio

123 Genetic selection maintains the altered phenotypes in su

124 rom the Finnish Twin Registry suggested that genetic selection may account for some of the physical-a

125 oped a rapid and broadly applicable in vitro genetic selection method based on T7 phage display.

126 This genetic selection method was shown to generate expressio

127 A genetic selection method, the P22 challenge-phage assay,

128 Genetic selection of borrelidin-resistant mutants showed

129 Genetic selection of cotransin-resistant cancer cells un

130 We have therefore developed a strategy for genetic selection of lineage-restricted precursors from

131 n interesting raw material for the design or genetic selection of modifiers of gene expression.

132 By the genetic selection of mouse cDNAs encoding secreted prote

133 ned to report mutations without the need for genetic selection of mutant cells.

134 The RNA challenge phage system enables genetic selection of proteins with RNA-binding activity

135 kinase originally identified in yeast by the genetic selection of rapamycin-resistant mutants.

136 on of LASV-LCMV domain-swapping experiments, genetic selection of viral variants, and site-directed m

137 The powerful genetic selections of the forward and reverse n-hybrid s

138 based screen performed by imposing different genetic selections on thousands of yeast mutants in para

139 d hydrophobic core sequence, imposed without genetic selection or computer-based design, is sufficien

140 t the processes of zoonotic transmission and genetic selection or modification ensure that plasma-der

141 lting libraries are subjected to large-scale genetic selection or screening to identify those chimera

142 ctional trade-offs may limit the capacity of genetic selection or synthetic biology to simultaneously

143 The technique makes use of a facile genetic selection performed in a strain of Saccharomyces

144 r, suggest that they have been adapted under genetic selection pressure for optimal performance.

145 An in vivo genetic selection previously developed to identify DNA b

146 unction of the glycosome, we used a positive genetic selection procedure to isolate the first Leishma

147 We have previously employed an in vitro (genetic) selection procedure to select RNase P ribozyme

148 s purified, we developed a modified in vitro genetic selection protocol.

149 Positive genetic selection provides a rapid mechanism to identify

150 aturation mutagenesis technique and positive genetic selection provides a simple and efficient means

151 Genetic selection provides the most powerful method to a

152 The genetic selections recapitulate major features of the co

153 onucleotide-directed mutagenesis followed by genetic selection resulted in mutant GlnRS enzymes that

154 Here we report a genetic selection revealing DinB residues essential to e

155 A genetic selection scheme against specific protein/protei

156 process called "pseudotaxis." Therefore, our genetic selection scheme generated suppressors of pseudo

157 We report here a simple genetic selection scheme through which to search librari

158 By a novel genetic selection/screening process, two BstYI variants

159 and scoring for fitness in plants by in vivo genetic selection (SELEX) resulted in winning sequences

160 Results of in vivo genetic selection (SELEX: systematic evolution of ligand

161 A quantitative genetic selection showed that +4 to +15 repeat expansion

162 We have developed a reliable genetic selection strategy for isolating interacting pro

163 ec7p and its interacting proteins, we used a genetic selection strategy in which a human HepG2 cDNA l

164 We devised a noninvasive genetic selection strategy to identify positive regulato

165 We have developed and used a genetic selection system in Escherichia coli to study fu

166 This system can be used as an efficient genetic selection system to map protein-protein interact

167 ic LacI protein as an example, we describe a genetic selection system using a bidirectional reporter

168 so demonstrates that the P22 challenge phage genetic selection system, modified for use with a mammal

169 in vitro translate directly into an in vivo genetic selection system.

170 Furthermore, because genetic selection takes place predominantly in the reduc

171 Although in vivo genetic selection techniques have been used to identify

172 yeast two-hybrid system is the provision for genetic selection techniques that enable the identificat

173 We have established a genetic-selection technology (intracellular antibody cap

174 is negative regulation, we developed a novel genetic selection that detects altered expression from t

175 Here we describe a genetic selection that directly links the in vivo stabil

176 By employing a genetic selection that forces the cell to fold an unstab

177 In this paper, we have utilized a genetic selection that links protein stability to antibi

178 hia coli that was previously identified by a genetic selection that rescued sensitivity to dithiothre

179 We created a genetic selection that ties the life of Escherichia coli

180 re and function in vivo, we have developed a genetic selection that ties the life of Escherichia coli

181 nd expression of these genes, we developed a genetic selection that uses transposon mutagenesis to id

182 Herein we describe genetic selections that allow single amino acid changes

183 terize the tagging process, we developed two genetic selections that link tmRNA activity to cell deat

184 Genetic selections that use proteinaceous transdominant

185 nd culture procedure, which facilitates, via genetic selection, the differentiation of hES cells into

186 We have used genetic selection to characterize the terminator of the

187 e of random mutagenesis followed by positive genetic selection to create such a mutant gene.

188 To test this hypothesis, we have designed a genetic selection to detect a leading strand intermolecu

189 oorly in many cell types, can be modified by genetic selection to generate a nonpathogenic variant th

190 This study demonstrates the power of a genetic selection to identify a variant virus that uses

191 ted mutagenesis procedures were coupled with genetic selection to identify an "oxygen-insensitive" mu

192 his type of approach to form the basis for a genetic selection to identify proteins that exert an act

193 We used this observation as the basis for genetic selection to identify two alleles of umuD' and s

194 In a novel genetic selection to isolate genes required for DIF sign

195 understand this patterning process, we used genetic selection to isolate mutants in the DIF-1 respon

196 nvolved in nuclear protein import, we used a genetic selection to isolate mutants that mislocalized a

197 dues at multiple positions and carried out a genetic selection to isolate variants that support life

198 RNA interference (RNAi) library in a forward genetic selection to study the mechanism of toxicity of

199 With this approach, we used powerful genetic selections to identify functional circuits and s

200 Here, using genetic selections to probe interactions of an exemplar

201 te these structures by using high-throughput genetic selections, unigenic evolution, and analyses of

202 We exploited a genetic selection using a dominant negative variant of t

203 We designed a genetic selection using cDNA library suppression of 3xAs

204 Genetic selections using P22 challenge phages were used

205 th kernel hardness, for which a new, simpler genetic selection was designed.

206 Genetic selection was exploited in combination with stru

207 albicans KEX2 homologue in Sap activation, a genetic selection was performed based on KEX2 function.

208 A genetic selection was performed for eps (ER-retained pma

209 e domain of DnaC that interacts with DnaB, a genetic selection was used based on the lethal effect of

210 associated with active site substitutions, a genetic selection was used to find second site mutations

211 Genetic selection was used to identify 33 amino acid sub

212 A novel genetic selection was used to identify genes regulating

213 substitutions in the AUGC repeats, in vitro genetic selection was used to identify RNAs that bound c

214 Bacterial genetic selection was used to isolate individual donor-t

215 d in transcription start site utilization, a genetic selection was used to isolate S. cerevisiae TFII

216 In vivo genetic selection was used to study the sequences and st

217 The combination of in vitro and in vivo genetic selections was crucial for obtaining RNA aptamer

218 Using PCR and genetic selection, we found that generalist and host-spe

219 Using a genetic selection, we have screened a random peptide lib

220 Using a genetic selection, we isolated an infectious retrovirus

221 By direct genetic selections, we identified mutations in 16S rRNA

222 tion of complexity, progressive and targeted genetic selections were employed to lessen and finally e

223 Genetic selections were used to find peptides that inhib

224 In the present study, yeast genetic selections were used to optimize the RNA aptamer

225 Inherent factors are determined by genetic selection, which appears to be controlled by ada

226 ecause domestication is a dynamic process of genetic selection, which may not be completely character

227 the ability to isolate intrabodies by direct genetic selection, which obviates the need for in vitro

228 Further, the genetic selection yields mutants at only a limited numbe