ライフサイエンスコーパス: genitalium

1 genomes (Buchnera aphidicola and Mycoplasma genitalium).

2 d 7 of 10 (70%) participants positive for M. genitalium.

3 onal repair pathway plays a minor role in M. genitalium.

4 more common among subjects infected with M. genitalium.

5 lammation was highest among subjects with M. genitalium.

6 onserved in other bacteria are missing in M. genitalium.

7 odel and assess endocervical infection by M. genitalium.

8 aginalis, and 47 (9.5%) were positive for M. genitalium.

9 of four geographically diverse strains of M. genitalium.

10 widely used to study the epidemiology of M. genitalium.

11 conserved in other bacteria are absent in M. genitalium.

12 MG_454 resists organic hydroperoxides in M. genitalium.

13 ouse DNA-based PCR assay for detection of M. genitalium.

14 ine minimum inhibitory concentrations for M. genitalium.

15 ited availability of diagnostic tests for M. genitalium.

16 We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoi

17 We observed a high prevalence of M. genitalium (7.4%) among HIV-infected New Orleans women.

18 Mycoplasma genitalium, a human pathogen associated with sexually tr

19 Mycoplasma genitalium, a human pathogen associated with sexually tr

20 erial vaginosis (BV) and incident Mycoplasma genitalium, a sexually transmitted bacterium associated

21 Mycoplasma genitalium, a sexually transmitted human pathogen, encod

22 h a 3.5-fold increase in odds of incident M. genitalium (adjusted odds ratio = 3.49, 95% confidence i

23 Mice infected with M. genitalium also developed specific antibodies to the maj

24 s the 582,970 base pair genome of Mycoplasma genitalium, although, as yet, this synthetic DNA has not

25 Screening and treatment of M. genitalium among HIV-infected individuals may be warrant

26 lationship between infection with Mycoplasma genitalium, an emerging sexually transmitted pathogen, a

27 le molecular methods for the diagnosis of M. genitalium and assays to predict the antibiotic suscepti

28 Prevalence of M. genitalium and associated genotypic markers of macrolide

29 ecently developed assay can test for both M. genitalium and azithromycin resistance mutations at the

30 compared with those reported for Mycoplasma genitalium and Bacillus subtilis.

31 infected women for an association between M. genitalium and cervicitis, a putative mechanism for enha

32 ly diverse clinical sites were tested for M. genitalium and for Chlamydia trachomatis, Neisseria gono

33 owing evidence for an association between M. genitalium and HIV genital shedding and the high prevale

34 strong epidemiologic associations between M. genitalium and human immunodeficiency virus (HIV), provi

35 To determine the prevalence of Mycoplasma genitalium and its association with cervical cytology an

36 The relatively high prevalence of Mycoplasma genitalium and its association with prevalent HIV urgent

37 ell division gene cluster in both Mycoplasma genitalium and its closest relative, Mycoplasma pneumoni

38 t groups of functionally related genes in M. genitalium and M. pneumoniae are often preceded by promo

39 Mycoplasma genitalium and other NGU pathogens were detected using n

40 Samples were tested for M. genitalium and other STI organisms (Chlamydia trachomati

41 g data demonstrate an association between M. genitalium and PID, and limited data suggest association

42 ns women, we retrospectively screened for M. genitalium and quantitatively characterized several mark

43 The M. genitalium and T. vaginalis detection rates among 755 pa

44 The associations between M. genitalium and the detection and quantity of genital HIV

45 lls expressing specific human TLR, viable M. genitalium and the recombinant C-terminal portion of the

46 athways involved in innate recognition of M. genitalium and the response to acute infection in the hu

47 ical EC are immunologically responsive to M. genitalium and to purified rMG309c via highly expressed

48 etermine if TMA could also detect Mycoplasma genitalium and Trichomonas vaginalis in men and women re

49 sing the following search terms: (Mycoplasma genitalium) AND (azithromycin OR zithromax OR [treatment

50 nsion of the STI analyte panel (including M. genitalium) and additional specimen source sampling with

51 achomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis infections as well

52 ibly small-two to four times smaller than M. genitalium-and these tiny genomes have raised questions

53 Mycoplasma pneumoniae and Mycoplasma genitalium are closely related organisms that cause dist

54 There is increasing concern about Mycoplasma genitalium as a cause of urethritis, cervicitis, pelvic

55 scovery, microbiology, and recognition of M. genitalium as a pathogen, and then summarize the recent

56 These findings implicate M. genitalium as an etiologic agent of cervicitis in HIV-in

57 ns, and the literature supporting Mycoplasma genitalium as an etiology of urethritis is growing.

58 Administration-approved clinical test for M. genitalium available in the United States at this time.

59 duplicates across seven bacteria (Mycoplasma genitalium, Bacillus subtilis, Helicobacter pylori, Haem

60 stream of these two genes was detected in M. genitalium but not M. pneumoniae.

61 cumulative PID incidence in women due to M. genitalium by 31.1% (95% range:13.0%-52.0%) over 20 year

62 MsrA affects the virulence properties of M. genitalium by modulating its interaction with host cells

63 m women every other month were tested for M. genitalium by nucleic acid amplification testing.

64 o Statens Serum Institut for detection of M. genitalium by polymerase chain reaction between 1 Januar

65 neration of genetic diversity observed in M. genitalium by the mutual exchange of sequences makes thi

66 field, the positive predictive values for M. genitalium, C. trachomatis, N. gonorrhoeae, and T. vagin

67 Here, we report that M. genitalium can establish long-term infection of human en

68 provide strong experimental evidence that M. genitalium can establish long-term infection of reproduc

69 The evidence that M. genitalium causes epididymo-orchitis, proctitis, and rea

70 Mycoplasma genitalium causes persistent urogenital tract infection

71 cquiring STIs, the prevalences of Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae

72 DNA sequence divergences among 54 Mycoplasma genitalium clinical strains isolated from the genitourin

73 itionally, the isolation of single-colony M. genitalium clonal variants containing alternative mgpB o

74 Chlamydia trachomatis and Mycoplasma genitalium coinfections were evaluated using nucleic aci

75 e inflammatory responses and suggest that M. genitalium colonization of reproductive tract tissues ma

76 V) infection revealed 19 (18.6%) cases of M. genitalium, commonly (58%) in rectal samples.

77 The DNA genome of Mycoplasma genitalium currently represents the smallest of all know

78 was no evidence of an association between M. genitalium detection or quantity and either plasma HIV-1

79 A 10.0% M. genitalium detection rate from other facilities exceeded

80 ch as the emergent human pathogen Mycoplasma genitalium, developed a complex polar structure, known a

81 g the search terms Mycoplasma genitalium, M. genitalium, diagnosis, and detection.

82 Typing assigned M. genitalium DLSTs to 2 major clusters, broadly distribute

83 ve polymerase chain reaction specific for M. genitalium DNA on samples 14-100 days post-treatment.

84 at and performance indices of a number of M. genitalium DNA- and RNA-based amplification assays; many

85 e and inflammation signature activated by M. genitalium during acute infection (48 hours after inocul

86 y, we demonstrate that surface-associated M. genitalium EF-Tu (EF-Tu(Mg)), in spite of sharing 96% id

87 tress response regulator in the genome of M. genitalium, elevated expression of MG_454 due to physica

88 ns, understanding the mechanisms by which M. genitalium elicits mucosal inflammation is an essential

89 The human pathogen Mycoplasma genitalium employs homologous recombination to generate

90 tive genomics has revealed that MG_454 of M. genitalium encodes a protein with putative function as a

91 identified STI involved sole detection of M. genitalium Expansion of the STI analyte panel (including

92 Here, we show that M. genitalium expresses N-terminally truncated RecA isoform

93 Recovery of viable M. genitalium from lower genital tract specimens was improv

94 -1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an a

95 The complete synthetic Mycoplasma genitalium genome ( approximately 583 kb) has been assem

96 haperone expression]) is predicted in the M. genitalium genome as well as three copies of its corresp

97 Approximately 4% of the limited M. genitalium genome contains repeat sequences termed MgPar

98 The Mycoplasma genitalium genome has historically defined the extreme s

99 ncing and de novo assembly of the Mycoplasma genitalium genome with 96% coverage at 99.96% accuracy i

100 y of recombination genes conserved in the M. genitalium genome.

101 e synthesized a 582,970-base pair Mycoplasma genitalium genome.

102 Several novel M. genitalium genotypes were identified, all of which were

103 Disruption of some genes accelerated M. genitalium growth.

104 Escherichia coli, inactivation of recA in M. genitalium had a minimal effect on survival after exposu

105 Testing for M. genitalium has become important for clinicians treating

106 available, and their use in screening for M. genitalium has been advocated, but M. genitalium's natur

107 Infection with Mycoplasma genitalium has been associated with male and female urog

108 Mycoplasma genitalium has been causally linked with nongonococcal u

109 Mycoplasma genitalium has been implicated in several important repr

110 It appears plausible that M. genitalium has the ability to generate unlimited variant

111 Since M. genitalium has the capacity to invade eukaryotic cells a

112 Mycoplasma genitalium has the smallest genome of any organism that

113 MG_454 to respond to oxidative stress in M. genitalium implies the absence of a known oxidative stre

114 eening programs and targeted treatment of M. genitalium improve reproductive outcomes in women are ne

115 al and extragenital screening for Mycoplasma genitalium in 102 asymptomatic Air Force members with hu

116 sequences vary within a single strain of M. genitalium in a pattern consistent with recombination be

117 mprehensive testing programs would detect M. genitalium in a significant proportion of females, parti

118 The importance of Mycoplasma genitalium in human immunodeficiency virus (HIV)-burdene

119 Although the pathogenic role of Mycoplasma genitalium in male urethritis is clear, fewer studies ha

120 nd the high prevalence and persistence of M. genitalium in this population suggest that further resea

121 al specimens for the detection of Mycoplasma genitalium in women by using our laboratory-developed PC

122 sitive specimen type for the detection of M. genitalium in women.

123 Factors associated with prevalent Mycoplasma genitalium, including sociodemographics, reproductive hi

124 aled reinfection by a different strain of M. genitalium, indicating the absence of protective immunit

125 igh azithromycin failure rate (39%) in an M. genitalium-infected cohort in association with high leve

126 The mean age of the M. genitalium-infected females (24.7 years) was lower than

127 Consecutive eligible M. genitalium-infected men and women attending the Melbourn

128 gimens in a prospective cohort of Mycoplasma genitalium-infected participants, and factors associated

129 train and in sequential specimens from an M. genitalium-infected patient.

130 At baseline, 16.1% had prevalent M. genitalium infection and 40.4% had prevalent BV.

131 determine the association between Mycoplasma genitalium infection and female reproductive tract syndr

132 quence variation in patients with chronic M. genitalium infection and to analyze the sequence structu

133 es assessing associations with persistent M. genitalium infection are needed.

134 employed to estimate the odds of incident M. genitalium infection at follow-up visits among women wit

135 Therefore, persistent M. genitalium infection could have important consequences f

136 Here, we assessed the incidence of M. genitalium infection in patients attending a sexually tr

137 and N. gonorrhoeae infections, while the M. genitalium infection rate in males was significantly hig

138 Cure of M. genitalium infection resulted in ablation of all signs o

139 se during menses were less likely to have M. genitalium infection than those who did not (odds ratio

140 Chronic M. genitalium infection was associated with increased secre

141 Mycoplasma genitalium infection was less prevalent in older women (

142 Mycoplasma genitalium infection was significantly associated with i

143 l STI (C. trachomatis, N. gonorrhoeae, or M. genitalium infection) was lower in the intervention arm,

144 nfection, and 0.66 [95% CI, .38-1.15] for M. genitalium infection).

145 female pig-tailed macaques as a model of M. genitalium infection, persistence, and immune evasion.

146 female pig-tailed macaque as a model for M. genitalium infection, we cervically inoculated eight add

147 tability of a pig-tailed macaque model of M. genitalium infection, we inoculated a pilot animal with

148 sts that BV may enhance susceptibility to M. genitalium infection.

149 nely screening any defined population for M. genitalium infection.

150 matis, Neisseria gonorrhoeae, and Mycoplasma genitalium infection.

151 There were 59 incident M. genitalium infections among 50 women, for an incidence r

152 igations into the causal relationships of M. genitalium infections and clinical disease have been hin

153 The prevalence rates of Mycoplasma genitalium infections and coinfections with other sexual

154 ly whether screening for and treatment of M. genitalium infections in women and their sexual partners

155 Significant risk factors for M. genitalium infections were black race, younger age, non-

156 Female M. genitalium infections were significantly more prevalent

157 ve useful in management of some resistant M. genitalium infections, although it is not likely to achi

158 vent the emergence of ever more resistant M. genitalium infections.

159 n, have been the treatments of choice for M. genitalium infections.

160 Mycoplasma genitalium is a common cause of nongonococcal urethritis

161 Mycoplasma genitalium is a common sexually transmitted infection as

162 Mycoplasma genitalium is a frequent undiagnosed cause of NGU in thi

163 Mycoplasma genitalium is a human bacterial pathogen linked to ureth

164 Mycoplasma genitalium is a human bacterial pathogen linked to ureth

165 Because Mycoplasma genitalium is a prevalent and emerging cause of sexually

166 Mycoplasma genitalium is a sexually transmitted pathogen associated

167 Antimicrobial-resistant M. genitalium is a significant problem and may require clin

168 Mycoplasma genitalium is an emerging sexually transmitted infection

169 Mycoplasma genitalium is an emerging sexually transmitted pathogen

170 Mycoplasma genitalium is an emerging sexually transmitted pathogen

171 Mycoplasma genitalium is an important and emerging agent of sexuall

172 Mycoplasma genitalium is an important sexually transmitted pathogen

173 Mycoplasma genitalium is an underappreciated cause of human reprodu

174 Because M. genitalium is associated with both HIV acquisition and t

175 Mycoplasma genitalium is associated with reproductive tract disease

176 Mycoplasma genitalium is associated with sexually transmitted infec

177 Mycoplasma genitalium is expected to metabolize RNA using unique pa

178 The prevalence of Mycoplasma genitalium is high in vulnerable populations of women in

179 Mycoplasma genitalium is increasingly appreciated as a common cause

180 Although Mycoplasma genitalium is increasingly recognized as a sexually tran

181 Mycoplasma genitalium is now recognized as a possible cause of seve

182 cubation period for NGU caused by Mycoplasma genitalium is probably longer than for NGU caused by C.

183 Mycoplasma genitalium is the smallest self-replicating bacterium an

184 Mycoplasma genitalium is the smallest self-replicating organism and

185 Mycoplasma genitalium is very difficult to grow in culture but has

186 region of MG192 was amplified by PCR from M. genitalium isolates obtained at various time points post

187 mbly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces ce

188 This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type

189 ve previously shown that a mutant form of M. genitalium lacking methionine sulfoxide reductase A (Msr

190 B and MgpC, but was insufficient to clear M. genitalium lower tract infection.

191 and 2016, using the search terms Mycoplasma genitalium, M. genitalium, diagnosis, and detection.

192 esting of high-risk symptomatic women for M. genitalium may be warranted.

193 Some studies suggest that M. genitalium may increase the risk of HIV acquisition.

194 Mycoplasma genitalium (MG) can cause nongonococcal urethritis and i

195 Mycoplasma genitalium (MG) is an emerging sexually transmitted infe

196 Mycoplasma genitalium (MG) is associated with nongonococcal urethri

197 Chlamydia trachomatis (CT), Mycoplasma genitalium (MG), and Trichomonas vaginalis (TV) are sexu

198 d for Chlamydia trachomatis (CT), Mycoplasma genitalium (MG), Ureaplasma urealyticum biovar 2 (UU-2),

199 of the third gene (MG192 or mgpC) of the M. genitalium MgPa adhesion operon, demonstrated that the M

200 An M. genitalium mutant lacking the MG491 segment correspondin

201 d more likely to shed HIV-1 DNA than were M. genitalium-negative women (adjusted OR, 2.9 [95% confide

202 es of MG_186, a calcium-dependent Mycoplasma genitalium nuclease.

203 arise from the introduction of diagnostic M. genitalium nucleic acid amplification testing including

204 are needed to better define the impact of M. genitalium on women's reproductive health.

205 s among subjects with monoinfections with M. genitalium or C. trachomatis compared to women with no d

206 Women with high M. genitalium organism burdens (more than the median of 319

207 h at most time points the median ratio of M. genitalium organisms to host cells was </=10, indicating

208 sequence analysis of single-colony cloned M. genitalium organisms.

209 (STI), 35.9% exhibited sole detection of M. genitalium (P </= 0.0004 versus sole detection of other

210 iled macaque is a suitable model to study M. genitalium pathogenesis, antibody-mediated selection of

211 In the upper tract, more than 90% of M. genitalium PCR-positive samples were from the uterus and

212 Viable M. genitalium persisted in the lower genital tract for 8 we

213 Genetic diversity of M. genitalium populations was determined to infer whether f

214 6.8% of women and 15.1% of men were found M. genitalium positive.

215 nce was detected in 38% (385/1008) of the M. genitalium-positive patients, and the highest rate was f

216 ess macrolide antibiotic resistance among M. genitalium-positive subjects.

217 Among the M. genitalium-positive women, the relative sensitivities of

218 Our results also suggest that M. genitalium possesses an active nucleotide excision repai

219 Surveillance of M. genitalium prevalence and antimicrobial resistance patte

220 M. genitalium prevalence rates were 16.1% for females and 1

221 Among 217 men, M. genitalium prevalence was 16.7% (95% confidence interval

222 Herein, we identify 382 of the 482 M. genitalium protein-coding genes as essential, plus five

223 We conclude that M. genitalium RecA has a primary role in mgpB/C-MgPar recom

224 s out the research priorities for Mycoplasma genitalium research agreed upon by the participants in a

225 esidual material was subjected to Mycoplasma genitalium research-use-only TMA.

226 Positive M. genitalium results were confirmed by repeat testing or a

227 atment, and public health significance of M. genitalium reviewed at the meeting is described in detai

228 Therefore, M. genitalium RNase R can precisely remove the 3'-trailer o

229 at the only exoribonuclease identified in M. genitalium, RNase R, is able to remove tRNA 3'-trailers

230 d these studies by examining the roles of M. genitalium ruvA and ruvB homologs.

231 o improve understanding of key aspects of M. genitalium's natural history before it will be possible

232 for M. genitalium has been advocated, but M. genitalium's natural history is poorly-understood, makin

233 owever, there is important uncertainty in M. genitalium's natural history parameters, leading to unce

234 behavioural studies to better understand M. genitalium's natural history, and then examined the effe

235 Mycoplasma genitalium samples from cases failing moxifloxacin were

236 itional Trichomonas vaginalis and Mycoplasma genitalium screening found 17.4% and 23.9% of the encoun

237 onsideration of the cost-effectiveness of M. genitalium screening interventions may be warranted.

238 These data suggest that M. genitalium selectively regulates a limited number of gen

239 These results show a high prevalence of M. genitalium single infections, a lower prevalence of coin

240 variation occurred during the process of M. genitalium single-colony cloning.

241 M. genitalium-specific IgG, but not IgA, was detected in ce

242 M. genitalium-specific serum antibodies targeting the immun

243 MG192 sequences were more related within M. genitalium specimens from an individual patient than bet

244 lium virulence, we compared the wild-type M. genitalium strain (G37) with an msrA mutant (MS5) strain

245 ganism, we examined mgpB variation within M. genitalium strain G-37 and observed sequence heterogenei

246 ection, we inoculated a pilot animal with M. genitalium strain G37 in the uterine cervix and in salpi

247 cimens from two chimpanzees infected with M. genitalium strain G37.

248 t mgpB variants within a single infecting M. genitalium strain, confirming that mgpB heterogeneity oc

249 gene is highly variable among and within M. genitalium strains in vitro and in vivo, and identified

250 terial pathogens, genomic diversity among M. genitalium strains worldwide is unknown.

251 nces, as well as mgpB variability between M. genitalium strains, suggests that mgpB and MgPar sequenc

252 all viable single-gene disruption Mycoplasma genitalium strains.

253 ese correlates differ from those found in M. genitalium studies conducted with FSW from West Africa a

254 ved except with superphysiologic loads of M. genitalium, suggesting that persistent infection occurs

255 e in a 5-year period where all diagnostic M. genitalium testing in Denmark was centralized at the Sta

256 tablish the cost-effectiveness of routine M. genitalium testing in symptomatic patients and screening

257 alth agencies should consider integrating M. genitalium testing into the management of persons with s

258 The aim of the study was to analyze the M. genitalium testing pattern and distribution of positive

259 use were more likely to be infected with M. genitalium than those who reported less frequent use (OR

260 life cycle of the human pathogen Mycoplasma genitalium that includes all of its molecular components

261 tion remains poorly understood in Mycoplasma genitalium, the smallest self-replicating cell and the c

262 In M. genitalium, this transcription terminates at two closely

263 We conclude that the M. genitalium TMA and PCR assays are highly specific and th

264 Incorporation of M. genitalium TMA into comprehensive testing programs would

265 estigated the transcriptional response of M. genitalium to elevated temperatures and detected the dif

266 This study highlights the capacity of M. genitalium to elicit cervical inflammation and, consider

267 Attachment of M. genitalium to the host cell's apical surface was observe

268 In addition, dissemination of M. genitalium to the knee tissues was observed as early as

269 In this study, we found that an M. genitalium transposon mutant that lacks expression of MG

270 Mycoplasma genitalium treatment failure was extremely common.

271 l and then inoculated intravaginally with M. genitalium type strain G37 or a contemporary Danish stra

272 han a single 1g dose at achieving cure of M. genitalium urethritis and importantly did not reduce the

273 e and new approaches for the treatment of M. genitalium urethritis are required.

274 microbiological cure in men with Mycoplasma genitalium urethritis during 2013-2015 and compared this

275 ological cure was determined for men with M. genitalium urethritis treated with azithromycin 1.5g usi

276 this study was to investigate if and how M. genitalium uses a minimal genome to generate genetic var

277 orkers in Kampala were tested for Mycoplasma genitalium using a commercial Real-TM polymerase chain r

278 se of the Gram-positive bacterium Mycoplasma genitalium using over 900 sources.

279 were tested for detection and quantity of M. genitalium using polymerase chain reaction analysis.

280 nto the mechanisms by which MsrA controls M. genitalium virulence, we compared the wild-type M. genit

281 The prevalence of M. genitalium was 12.9%.

282 The prevalence of Mycoplasma genitalium was 14% and higher in HIV-positive women than

283 M. genitalium was also less prevalent among FSW who had wor

284 Mycoplasma genitalium was associated with Neisseria gonorrhoeae (ad

285 clear, and we sought to determine whether M. genitalium was capable of activating innate immune respo

286 chain reaction (PCR) in 154 women (51%); M. genitalium was detected by qualitative PCR in 52 (17%),

287 Among the women, M. genitalium was detected by the TMA and PCR assays in 36

288 M. genitalium was detected in 10.5% of stored specimens (44

289 Among the men, M. genitalium was detected in 24 urine specimens (6.8%) by

290 M. genitalium was detected in 282 (11.4%) patients.

291 M. genitalium was independently associated with detection o

292 n-PCR showed that expression of MG_454 in M. genitalium was not elevated in response to oxidative str

293 inoculated in salpingeal pockets, viable M. genitalium was recovered for 2 weeks.

294 Using a novel quantitative PCR assay, M. genitalium was shown to replicate from 0 to 80 days post

295 ce building a whole-cell model of Mycoplasma genitalium, we identified several significant challenges

296 f 22 (41%; 95% CI, 20%-62%) patients with M. genitalium were infected with DLSTs possessing genotypic

297 med at Chlamydia trachomatis, but Mycoplasma genitalium, which also commonly causes undiagnosed NGU,

298 ional organization of the RuvAB system of M. genitalium, which is cotranscribed with two novel open r

299 ells, MG_186 has the potential to provide M. genitalium, which possesses the smallest genome of any s

300 mens from 28 958 patients were tested for M. genitalium, with an increasing trend from 3858 per year