ライフサイエンスコーパス: genome editing

1 enes (SpCas9) has been widely repurposed for genome editing.

2 with AAV donor vectors for homology-directed genome editing.

3 ve been harnessed as a robust technology for genome editing.

4 against epitope tags inserted by CRISPR/Cas9 genome editing.

5 atives toward establishing model systems for genome editing.

6 ed vimentin with an mEmerald tag using TALEN genome editing.

7 nition and nuclease activation for precision genome editing.

8 e an effective strategy to enhance precision genome editing.

9 rly important for gene therapy and precision genome editing.

10 rcial cultivars, if non-functional, based on genome editing.

11 the heterologous SpCas9 system favoured for genome editing.

12 endonucleases in the context of therapeutic genome editing.

13 (LbCpf1) have been harnessed for eukaryotic genome editing.

14 ciferase reporter using CRISPR/Cas9-mediated genome editing.

15 st-translational modulation of Cpf1-mediated genome editing.

16 icability of CRISPR-Cas regulatory tools for genome editing.

17 of potential clinical uses of human germline genome editing.

18 nt of a variety of new technologies, such as genome editing.

19 (crRNA) can be used to simplify multiplexed genome editing.

20 design of synthetic donor DNAs for efficient genome editing.

21 to increase efficiency of HDR-based precise genome editing.

22 stem has been used for efficient multiplexed genome editing.

23 blish a method termed 'CORRECT' for scarless genome editing.

24 tivity profile, has changed the landscape of genome editing.

25 system that has been recently harnessed for genome editing.

26 increased apoptosis, rescued by CRISPR/Cas9 genome editing.

27 use zygotes with 100% efficiency for in vivo genome editing.

28 ible for monogenic neuromuscular diseases by genome editing.

29 the guide, which poses a major challenge for genome editing.

30 rify phenomena associated with Cas9-mediated genome editing.

31 ) with yeast as an intermediary host for the genome editing.

32 expanding the target range of Cpf1-mediated genome editing.

33 o direct Cas9-mediated allotetraploid cotton genome editing.

34 overnance of clinical applications involving genome editing.

35 specific gene insertion by homology-directed genome editing.

36 ap35KI isogenic iPSC lines using CRISPR/Cas9 genome editing.

37 ions into the mouse germline by CRISPR-based genome editing.

38 portant also as tools for gene targeting and genome editing.

39 s can serve as off switches for CRISPR-based genome editing (5) .

40 n be modified while maintaining or enhancing genome-editing activity, and we develop an optimal set o

41 that nanocarriers delivering mRNA encoding a genome-editing agent can efficiently knock-out selected

42 Programmable sequence-specific genome editing agents such as CRISPR-Cas9 have greatly a

43 d, both in vitro and in primary fibroblasts, genome editing agents that preferentially disrupt the do

44 Although canonical forms of genome-editing agents and programmable transcriptional r

45 strategy that builds upon recent advances in genome editing and combines ex vivo and in vivo chromoso

46 transposase resulted in efficient, targeted genome editing and concurrent scarless transgene excisio

47 rt palindromic repeats-associated protein 9) genome editing and confirmed functional disruption of th

48 te KRAS inhibition using CRISPR/Cas-mediated genome editing and demonstrate that KRAS is dispensable

49 CRISPR-Cas9 provides the means to perform genome editing and facilitates loss-of-function screens.

50 f programmable meganucleases is transforming genome editing and functional genomics.

51 pularity in using CRISPR/Cas9 technology for genome editing and gene knockout, its performance still

52 system-wide biophysical model of Cas9-based genome editing and gene regulation to predict how changi

53 J repair and have important implications for genome editing and genome evolution.

54 By using CRISPR-Cas9 genome editing and in vitro smooth muscle differentiatio

55 en developed into numerous applications like genome editing and regulation of transcription in eukary

56 latform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivative

57 small molecule-controlled nuclease-mediated genome editing and small molecule-controlled base editin

58 which this knowledge may be manipulated for genome editing and synthetic biology purposes.

59 SPR-based technologies that enable mammalian genome editing and their various applications.

60 stem, has been widely adopted for RNA-guided genome editing and transcription regulation in applicati

61 e, precise temporal and spatial control over genome editing and transcriptional regulation activities

62 Here we implement CRISPR-Cas9 genome editing and transposon-mediated somatic gene tran

63 With genome-editing and mouse genetics approach, we show that

64 Finally, genome-editing and transgenic approaches demonstrate tha

65 )-Cas nuclease system is a powerful tool for genome editing, and its simple programmability has enabl

66 V2) are powerful tools for gene transfer and genome editing applications.

67 se otherwise inactive CRISPR-Cas systems for genome-editing applications and a potential path to modu

68 nsidering individual genomes for therapeutic genome-editing applications for the design and evaluatio

69 Adaptation of CRISPR-Cas9 for genome-editing applications has revolutionized biomedica

70 mammalian cells and are thus precluded from genome-editing applications.

71 This genome editing approach has the advantage that it does n

72 Here we report a genome editing approach in which adeno-associated virus

73 We recently developed base editing, a genome-editing approach that enables the programmable co

74 We developed a genome-editing approach to target a dominantly inherited

75 or HIV therapy and summarize other promising genome editing approaches for future clinical developmen

76 By combining our inducible and multiplex genome editing approaches, we were able to simultaneousl

77 materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies,

78 Objectives: To provide an overview of genome-editing approaches; to summarize published report

79 echnologies, successful somatic and germline genome editing are becoming feasible.

80 Now, CRISPR-Cas9 tools for site-specific genome editing are needed to facilitate further improvem

81 These mutant collections, together with genome editing, are being used in polyploid species to c

82 lating technologies, like gene silencing and genome editing, are providing ability to understand in d

83 udy provides evidence for using CRISPR-based genome editing as a potential therapeutic approach for r

84 This work establishes CRISPR-Cas9-based genome editing as a potential therapy to treat DMD.

85 his work establishes a strong foundation for genome editing as a strategy to treat angiogenesis-assoc

86 increasing and evaluating the efficiency of genome editing based on the CRISPR-Cas9 (clustered regul

87 This study provides a genome editing-based multiplex strategy for direct funct

88 the benefits to be had are enormous, such as genome editing, but ethical concerns persist.

89 CRISPR-Cas9 is a powerful new tool for genome editing, but this technique creates mosaic mutati

90 Here we directly compare genome editing by CRISPR-Cas9 (cutting, CRISPRc) and gen

91 Genome editing by CRISPR/Cas9 revealed that OCT4 and SOX

92 ucleases, such as Cas9, are used for precise genome editing by homology-dependent repair (HDR).

93 Here, using multiplex genome editing by natural transformation (MuGENT), we sy

94 ecently, we described a method for multiplex genome editing by natural transformation (MuGENT).

95 In summary, this study shows how multiplex genome editing can be used to rapidly dissect complex bi

96 CRISPR/Cas9-based genome editing can easily generate knockout mouse models

97 ally high precision is driven by advances in genome editing, cellular reprogramming, tissue engineeri

98 accination, protein replacement therapy, and genome editing, collectively affecting approaches for th

99 ept of metabolic disease modeling by somatic genome editing could be applied to many other systemic a

100 ults suggest that TCR transfer combined with genome editing could lead to new, improved generations o

101 creening paradigm exploiting CRISPR-mediated genome editing coupled to a cell selection step by FACS

102 aced short palindromic repeat)/Cas9-mediated genome editing coupled with human pluripotent stem cell

103 esis, gene silencing (RNA interference), and genome editing (CRISPR/Cas9) approaches in Caenorhabditi

104 June 9, 2016, using the following keywords: genome editing, CRISPR-Cas9, neuromuscular disease, Duch

105 New genome editing development, such as using CRISPR/cas9, o

106 ull cells, generated by CRISPR/Cas9 nuclease genome editing, display an abrogated stretch-stimulated

107 CRISPR/Cas9 is a promising tool for genome-editing DNA in cells with single-base-pair precis

108 GN) has emerged to be a versatile method for genome editing due to the ease of construction of RGN re

109 chhiking mutations and context-dependence of genome editing efficiency that would confound other stra

110 We showed that CRIPSR-mediated genome editing efficiently excised the mutant exon 23 in

111 Recent advances in genome editing enable efficient sequence-specific interv

112 CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knoc

113 ISPR/Cas-blocking mutations in two rounds of genome editing, enables accurate, efficient and scarless

114 asily accessible to anyone planning a CRISPR genome editing experiment, we built a new website that p

115 ne web tool that can be used in a variety of genome editing experimental contexts.

116 The genome-editing field has advanced to a remarkable degree

117 region as a potential target for therapeutic genome editing for hemoglobinopathies and highlight the

118 This study demonstrates the application of genome editing for targeted integration of human imaging

119 ccessful application of CRISPR-Cas9-mediated genome editing for the correction of a TGFBI mutation in

120 Here we describe a genome-editing framework termed consecutive re-guide or

121 Here we describe a CRISPR/Cas9-based genome-editing framework that allows selective introduct

122 R/Cas9), resulted in a much greater targeted genome-editing frequency compared with treatment with DN

123 target tissue for transgene integration and genome editing, friable embryogenic callus (FEC).

124 CRISPR/Cas9 genome editing generated predicted null mutations in cnr

125 CRISPR/Cas9 genome editing has been proposed as a therapeutic treatm

126 Germline manipulation using CRISPR/Cas9 genome editing has dramatically accelerated the generati

127 Genome editing has emerged as a technology with a potent

128 The revolution in CRISPR-mediated genome editing has enabled the mutation and insertion of

129 The introduction of Cas9-directed genome editing has expanded adoption of this approach.

130 Genome editing has potential for the targeted correction

131 Although Cas9-mediated genome editing has proven to be a powerful genetic tool

132 Although CRISPR/Cas9 genome editing has provided numerous opportunities to in

133 CRISPR/Cas9 genome-editing has emerged as a powerful tool to create

134 Sequenced mutant populations and genome editing have changed the paradigm of what is poss

135 Inexpensive DNA sequencing and advances in genome editing have made computational analysis a major

136 enomes, high-throughput omics profiling, and genome editing, have begun to elucidate plant terpene me

137 CRISPR/Cas9-mediated genome editing holds clinical potential for treating gen

138 s9 is a powerful technology that has enabled genome editing in a wide range of species.

139 iated HDR will be broadly useful for precise genome editing in basic and translational neuroscience.

140 We use CRISPR-Cas9-mediated genome editing in cultured human TM cells and in a MYOC

141 Using genome editing in Drosophila, we show that Atonal outliv

142 ed endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells.

143 s emerged as a simple and efficient tool for genome editing in eukaryotic cells.

144 expression that allows a variety of ex vivo genome editing in fibroblast cells including single- and

145 at increases the efficiency of HDR-dependent genome editing in human and mouse cells.

146 esis and myeloid disorders using CRISPR-Cas9 genome editing in human hematopoietic stem and progenito

147 We utilized CRISPR/Cas9 genome editing in human induced pluripotent stem (iPS) c

148 optimised methods enable facile and scalable genome editing in mammalian NSCs, providing significant

149 ncreased the accessibility and efficiency of genome editing in many organisms.

150 have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling e

151 ated with CHD, were validated by CRISPR-Cas9 genome editing in mice as being digenic causes of HLHS.

152 NA in vivo, is a commonly used technique for genome editing in microbes.

153 Gang et al. report CRISPR/Cas9 genome editing in parasites of the genus Strongyloides,

154 To further facilitate genome editing in pigs, we report here establishment of

155 These advances indicate the potential of genome editing in the brain to correct or inactivate the

156 tem, we demonstrate the feasibility of human genome editing in the eye for this important disease.

157 is strategy may enable non-viral, Cas9-based genome editing in the liver in clinical settings.

158 genetic screening approach using CRISPR-Cas9 genome editing in transplantable tumours in mice treated

159 and assessed their utility for site-specific genome editing in two insect cell lines commonly used as

160 -KO cell lines were generated by CRISPR/Cas9 genome editing in U2OS cells.

161 adjacent motif (PAM) and is widely used for genome editing in various organisms.

162 to test enhancer-promoter interactions, and genome editing in vitro to show allele-specific effects

163 the feasibility of CRISPR/Cas9-based cardiac genome editing in vivo in postnatal mice.

164 Here we report a method for CRISPR-mediated genome editing in Xenopus oocytes with homology-directed

165 SLA class I is a target for genome editing in xenotransplantation.

166 resents a strategy for precise and effective genome editing in zebrafish.The use of base editing enab

167 We conclude that CRISPR-Cas9-mediated genome editing is a powerful method for investigating ge

168 Conclusions and Relevance: Genome editing is a rapidly evolving technology with eno

169 In addition, genome editing is becoming increasingly plausible as a t

170 Genome editing is expected to result in changes of the s

171 CRISPR/Cas9 genome editing is revolutionizing genetic loss-of-functi

172 cally modified animals, CRISPR/Cas9-mediated genome editing is typically accomplished by microinjecti

173 echniques rather than attempting to regulate genome editing itself as a new technology.

174 lly clarifying the biophysics of this unique genome editing machinery and at developing new tools for

175 udies reveal the feasibility and efficacy of genome-editing-meditated correction of monogenic neuromu

176 Our genome-editing methodology using Cas9/crRNA ribonuclear

177 feasibility, efficacy, and safety of current genome-editing methods as they relate to the potential c

178 CRISPR-Cas genome-editing methods hold immense potential as therape

179 cleases, up to 8%, is higher than most other genome editing nucleases, indicative of its effective en

180 tem holds enormous potential for therapeutic genome editing of a wide spectrum of diseases.

181 Using CRISPR-Cas9 genome editing of bptf in zebrafish to induce a loss of

182 expressed from a single U6 promoter to exert genome editing of dystrophin and myosin binding protein

183 Genome editing of human induced pluripotent stem cells (

184 used CRISPR-Cas9 (CRISPR-associated protein) genome editing of Lmod1 to generate a similar premature

185 Precise genome editing of plants has the potential to reshape gl

186 Here we demonstrate the use of CRISPR/Cas9 genome editing of primary human hematopoietic stem/proge

187 Here, we demonstrate that CRISPR/Cas9 genome editing of promoters generates diverse cis-regula

188 Genome editing of the neurokinin 1 receptor (NK1R) in th

189 itigated through pharmacologic inhibition or genome editing of these loci.

190 either transient suppression or CRISPR/Cas9 genome editing of zebrafish tmem260 recapitulated key ne

191 Both knockdown and genome editing of znhit3 in zebrafish embryos recapitula

192 We labeled endogenous EGFR with GFP by genome-editing of human oral squamous cell carcinoma cel

193 CRISPR/Cas9-based genome editing offers the possibility to knock out almos

194 and physiological impact of CRISPR-mediated genome editing on cardiac dystrophin expression and func

195 erspaced short palindromic repeats)-mediated genome editing on dystrophin expression and cardiac func

196 e is no reason to prohibit in vitro germline genome editing on human embryos and gametes, with approp

197 e lesions facilitate high-efficiency precise genome editing (PGE) via homology-directed repair (HDR)

198 ) and an engineered single guide RNA (sgRNA) genome editing platform that offers revolutionary soluti

199 dings show the enormous potential of using a genome-editing platform for precise, reliable trait deve

200 ed short palindromic repeat (CRISPR)-derived genome editing provide an unprecedented opportunity to p

201 Nuclease-driven genome editing provides a method in which to precisely t

202 As genome editing rapidly progresses toward the realization

203 rticular, recent advances in the delivery of genome editing reagents and the demonstration of highly

204 the author discusses the basic principles of genome editing, recent advances in clustered regularly i

205 An EASAC working group on genome editing recommends that regulators should focus o

206 Cas9-mediated genome editing reduces the time needed to construct muta

207 We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other p

208 Precise genome-editing relies on the repair of sequence-specific

209 ent stem cells (iPSCs) and nuclease-mediated genome editing represent a unique opportunity for studyi

210 Realizing the full potential of genome editing requires the development of efficient and

211 Knockin of specific mutations (precision genome editing) requires homology-directed repair (HDR)

212 In vivo genome editing results in lower IOP and prevents further

213 Epigenomic profiling and genome editing revealed that AMIGO2 is regulated by a me

214 ar machines have been repurposed to enable a genome editing revolution.

215 SPR) has recently become synonymous with the genome-editing revolution.

216 uture clinical application of human germline genome editing should not proceed unless, at a minimum,

217 The efficient genome editing shown here demonstrates that these pigs c

218 le robust and highly efficient Cas9-directed genome editing, so that a parental line can be expeditio

219 an enable further optimization of Cas9-based genome-editing specificity and efficiency.

220 Here we argue that combined advances in genome editing, stem cell production, and organoid deriv

221 ance of in vitro models as tools to validate genome editing strategies before clinical application.

222 estigating T cell development and validating genome editing strategies in vitro.

223 To address this problem, we used genome-editing strategies to investigate C9orf72 interac

224 eveloped a high-throughput CRISPR/Cas9-based genome-editing strategy and used it to interrogate 174 c

225 We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous

226 We show through transgenic reporter and genome-editing studies in mice that Ihh is regulated by

227 We used the CRISPR-Cas9 genome editing system and homology directed repair to is

228 The CRISPR-Cas9 genome-editing system is a part of the adaptive immune s

229 fe and efficient delivery of the CRISPR-Cas9 genome-editing system to target cells in human body.

230 Current genome-editing systems generally rely on inducing DNA do

231 he adaptation of the CRISPR-Cas9 system as a genome editing technique has generated much excitement i

232 s been shown to be an efficient and accurate genome-editing technique.

233 Then, we discuss how genome editing techniques enable a radically new set of

234 unities for the control of potyviruses using genome editing techniques targeted on eIF2Bbeta.

235 In an effort to develop novel genome editing techniques that safely excise HIV proviru

236 ire further research and proposes the use of genome editing technologies for engineering disease resi

237 trates the powerful combination of iPSCs and genome editing technologies for understanding the biolog

238 orts on the application of this or any other genome editing technologies in the cotton plant.

239 ystems is revolutionizing the utilization of genome editing technologies in the study of molecular co

240 Recent advancement in genome editing technologies offers a promising therapeut

241 R RNA (crRNA), and it has been harnessed for genome editing technologies.

242 iations, induced mutations, and the advanced genome-editing technologies can be applied to improving

243 Current genome-editing technologies introduce double-stranded (d

244 With CRISPR/Cas9 and other genome-editing technologies, successful somatic and germ

245 g outcomes, and compare them to other CRISPR genome-editing technologies.

246 r refinements and broad adoption of the Cpf1 genome editing technology have the potential to make a d

247 Successful establishment of CRISPR/Cas9 genome editing technology in Plasmodium spp. has provide

248 CRISPR-Cas9 is a genome editing technology with major impact in life scie

249 CRISPR-Cas9 has become a facile genome editing technology, yet the structural and mechan

250 CRISPR-Cas9 is a powerful genome editing technology, yet with off-target effects.

251 of Copb2 in neural development, we utilized genome-editing technology to generate an allelic series

252 in regulating centrosome activities, we used genome editing to ablate it.

253 and underscore the potential of CRISPR/Cas9 genome editing to advance immunotherapies.

254 tors that were engineered by CRISPR-mediated genome editing to controllably release GLP-1 (glucagon-l

255 oolkits, with wide-ranging applications from genome editing to diagnostic tools based on various Cas

256 Here we applied CRISPR-Cas9 genome editing to disrupt the endogenous human MRP RNA l

257 In this study we used genome editing to generate clonal HEK293 (Hrd1.KI) cells

258 Here, we used Cas9-based genome editing to introduce the gene encoding the prodru

259 Here we use CRISPR-Cas9-mediated genome editing to investigate the function of the plurip

260 In this study, we used genome editing to knockout the two mcoln1 genes present

261 Towards this end, we used CRISPR-Cas9 genome editing to make a single allele knock-in of the m

262 Here we used CRISPR/Cas9 genome editing to separate catalytic activity-dependent

263 Here we used CRISPR/Cas9 genome editing to show that optix plays a fundamental ro

264 s for the CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to recruit

265 ts (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals.

266 endonuclease system is a powerful RNA-guided genome editing tool.

267 s been harnessed as a powerful and versatile genome-editing tool and holds immense promise for future

268 describe the development of a Cas9-mediated genome-editing tool that allows facile genetic manipulat

269 The CRISPR-Cas system owes its utility as a genome-editing tool to its origin as a prokaryotic immun

270 limited the capabilities of the RNP-mediated genome editing toolbox.

271 the stage for development of a new class of genome editing tools based on directed deamination of 2-

272 Genome editing tools have revolutionized the generation

273 Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted le

274 oped osteocytic cell lines-together with new genome editing tools-has allowed a closer look at the bi

275 directed repair-dependent and NHEJ-dependent genome-editing tools comprises a powerful genetic system

276 Here, TALEN and CRISPR-Cas9, two versatile genome-editing tools, are employed to target common carp

277 targeted chromatin conformation capture and genome editing uncovers how NF-kappaBeta that has just e

278 acoustic-transfection technique for precise genome editing using CRISPR-Cas9.

279 Genome editing using programmable nucleases has revoluti

280 However, marker-free genome editing using standard protocols remains ineffici

281 mutation was corrected by CRISPR/Cas9-based genome editing (V247fs-MT-correction).

282 rate, through ex vivo and proof of principle genome editing validation, that variants in super enhanc

283 Here we show that precise genome editing via HDR is possible in mature postmitotic

284 Precise genome editing via homology-directed repair (HDR) after

285 Precise genome editing via homology-directed repair (HDR) in tar

286 Single-cell, HDR-mediated genome editing was achieved by delivering the editing ma

287 Using CRISPR-Cas9-mediated genome editing we find that BAZ1A and BAZ1B each recruit

288 nditions, stepwise deletions and marker-less genome editing, we found that SigX is the missing link i

289 k-in mouse generated by CRISPR/Cas9-mediated genome editing, we found that the endogenous Zfp36 direc

290 ba-seq with multiplexed CRISPR-Cas9-mediated genome editing, we quantified the effects of 11 tumor-su

291 ene function, and CRISPR technology has made genome editing widely accessible in model organisms and

292 variation [e.g., allelic profiling and (epi)genome editing] will be critical to dissect the molecula

293 Efficient genome editing with Cas9-sgRNA in vivo has required the

294 Genome editing with designer nucleases such as TALEN and

295 d human cells enables gene recombination and genome editing with efficiencies greater than 70%.

296 targeted" metabolomic strategy that combines genome editing with pathway analysis to probe the functi

297 ficient toolbox provides a solution for easy genome editing with tight temporal control, minimal off-

298 show that ablation of zebrafish f10 by using genome editing with transcription activator-like effecto

299 e as a cautionary note that CRISPR -mediated genome editing without full knowledge of genomic context

300 paludicola, allowed efficient Cas9-mediated genome editing without the need for a repair template.