ライフサイエンスコーパス: genomic DNA

1 ancient organisms that lack large amounts of genomic DNA.

2 p dsDNA analytes, including both plasmid and genomic DNA.

3 ive cell birth dating measuring carbon-14 in genomic DNA.

4 that mediate the conversion of A*T to G*C in genomic DNA.

5 reventing the hyper-negative supercoiling of genomic DNA.

6 of protein-DNA interactions on synthetic and genomic DNA.

7 en the expanded repeat RNA and complementary genomic DNA.

8 n assessing global modification abundance in genomic DNA.

9 ng IGS sequencing data with RFLP analysis of genomic DNA.

10 and reduced the levels of oxidized bases in genomic DNA.

11 ization, providing a mechanism for accessing genomic DNA.

12 intaining the placement of nucleosomes along genomic DNA.

13 3 KIR genes and HLA-A, HLA-B, and HLA-C from genomic DNA.

14 s can be processed to extract high yields of genomic DNA.

15 patients by means of targeted sequencing of genomic DNA.

16 data, which is related to the copy number of genomic DNA.

17 judged from binding competition against bulk genomic DNA.

18 catalyzes cytosine methylation of mammalian genomic DNA.

19 3 hypervariable region from Escherichia coli genomic DNA.

20 result in accumulation of ribonucleotides in genomic DNA.

21 om whole-genome amplification of single-cell genomic DNA.

22 centrations of variant alleles (1%) in human genomic DNA.

23 is required for TLR9 activation by bacterial genomic DNA.

24 ften involve amplifications and deletions of genomic DNA.

25 iptional consequences from ribose-containing genomic DNA.

26 s an initial quality assessment of amplified genomic DNA.

27 FIP registry for whole-exome sequencing from genomic DNA.

28 profiling of ribonucleotide incorporation in genomic DNA.

29 to identify the sequence of cut sites within genomic DNA.

30 on of approximately 30 copies of the CaMV35S genomic DNA.

31 ure of target DNA from a pool of interfering genomic DNA.

32 stranded breaks (DSBs) in murine macrophage genomic DNA.

33 moval of UV-induced direct photolesions from genomic DNA.

34 ctivity by controlling its ability to access genomic DNA.

35 nely evaluated with a PCR-based method using genomic DNA.

36 reposition and reorganize nucleosomes along genomic DNA.

37 etion and reduced the L1-mediated nicking of genomic DNA.

38 pecific amplification by directly sequencing genomic DNA.

39 es and sequences the 4.8-Mbp MHC region from genomic DNA.

40 med Cas9 locates the target site by scanning genomic DNA.

41 ed DNAs, including circular plasmid DNAs and genomic DNAs.

42 l factories localized with replicating viral genomic DNAs.

43 ly sensitive with detection limit of 20fg of genomic DNA (3-4 pathogens).

44 ct human genomic DNA down to a mass of human genomic DNA (5.5 picograms) that is roughly equal to the

45 -kb fragment from different sources of human genomic DNA, a 1.9-kb fragment was sometimes coamplified

46 ymethylated CpG sites (5hmC/5mCpGs) in mouse genomic DNA across multiple tissues.

47 Furthermore, the injection of mouse genomic DNA alone is sufficient to recapitulate many fea

48 Various investigators, using genomic DNA analyses, have linked -344T polymorphism in

49 Whole heart genomic DNA analysis revealed iterative oxidative cytosi

50 reference/consensus IGS sequence, as well as genomic DNA analysis.

51 The limits of detection were 0.5ng/ml of genomic DNA and 10 colony-forming units (CFU)/ml of bact

52 xes in eukaryotes that provide compaction of genomic DNA and are implicated in regulation of transcri

53 ) and 5 x 10(4) cells/ml were obtained using genomic DNA and bacterial culture from M. capricolum sub

54 S patients, which indeed accumulate rNMPs in genomic DNA and exhibit markers of constitutive PRR and

55 od for separation and parallel sequencing of genomic DNA and full-length polyA(+) mRNA from single ce

56 Using a reconstituted system containing genomic DNA and purified proteins from yeast, Krietenste

57 nsuming and often requires a large amount of genomic DNA and radioactively labeled probes.

58 important cytosolic sensor of P. falciparum genomic DNA and reveal the role of the cGAS/STING pathwa

59 In a retrospective, case-control study, genomic DNA and serum were extracted from blood collecte

60 % and cassette function can be traced at the genomic DNA and the mRNA level.

61 st abundant non-canonical component of yeast genomic DNA and their persistence is associated with a d

62 cleotides that have a specific target on the genomic DNA and their signals are derived from specific

63 e of the MinION nanopore sequencer using M13 genomic DNA and used expectation maximization to obtain

64 ease in OGG1-sensitive oxidized bases within genomic DNA, and a decrease in 8-oxoG cleavage activity

65 from slow turnaround, inefficient capture of genomic DNA, and high cost.

66 PCR analysis revealed presence of bacterial genomic DNA, and infection elicited significant immunogl

67 druplex structures may act as 'knots' within genomic DNA, and it has been hypothesized that proteins

68 -reporter mice, polymerase chain reaction of genomic DNA, and quantitation of Bmp6 messenger RNA expr

69 rich sequences that are excised from damaged genomic DNA are proposed to enter the cytoplasm where th

70 ymerase chain reaction (qPCR) to detect HAdV genomic DNA as a means to quantify attachment and genome

71 and hydroxyl radicals in vivo, and protected genomic DNA as well as sensitive enzymes from intracellu

72 e ribonucleoside monophosphates (rNMPs) into genomic DNA at a low level and such rNMPs are efficientl

73 on restriction enzymes capable of digesting genomic DNA at defined sequence motifs.

74 method for the electrochemical detection of genomic DNA, based on the employment of two sub-micron o

75 d with various concentrations of Aspergillus genomic DNA before extraction following international re

76 T or LGT) is the transmission of portions of genomic DNA between organisms through a process decouple

77 Personalized arms using a "genomic (DNA) biomarker" had higher median RR than those

78 in complementation version of DamID, to mark genomic DNA bound in vivo by interacting or juxtapositio

79 re anucleate cytoplasmic fragments that lack genomic DNA, but continue to synthesize protein using a

80 geal specimens were tested for HBoV mRNA and genomic DNA by quantitative polymerase chain reaction.

81 ranscription factors that bind to regions of genomic DNA called enhancers.

82 Stable formation of GQ in genomic DNA can be counteracted by the resolving activit

83 Structural variations (SVs) in genomic DNA can have profound effects on the evolution o

84 Genomic DNA capture and sequencing of a modifier haploty

85 le blood proves that the large mass of human genomic DNA captured from the lysed cells does not inhib

86 ated gold-nanoparticles interacting with the genomic DNA captured in the fiber channels (0%, 0.1%, 1%

87 The target complementary genomic DNA (cDNA) isolated from clinical samples of V.

88 A demonstration with PCR-amplified genomic DNA confirms on-chip detection and identificatio

89 Genedrive assay had an LOD of 1 pg/mul (100 genomic DNA copies/reaction).

90 eral layers of regulation prevent collateral genomic DNA damage by restricting RAG activity to the G1

91 NCC is genetically distinct from its role in genomic DNA damage repair.

92 plasma DNA sequencing data with the parental genomic DNA data and using a series of bioinformatics fi

93 e, we demonstrate that Plasmodium falciparum genomic DNA delivered to the cytosol of human monocytes

94 ple invasion-suppressor miRNAs by inhibiting genomic DNA demethylation by direct targeting of TET1, t

95 s for the selective and ultrasensitive human genomic DNA detection, directly extracted from lymphocyt

96 properties of different functional units on genomic DNA differ in their signatures.

97 ws the RDM-based pinwheel assay detect human genomic DNA down to a mass of human genomic DNA (5.5 pic

98 cumented using plasmid, viral, or fragmented genomic DNA; dPCR performance with more complex material

99 Thus, genomic DNA DSBs act as signaling intermediates in murin

100 and-passaging activity that resolves tangled genomic DNA during mitosis.

101 The ability to rewrite large stretches of genomic DNA enables the creation of new organisms with c

102 Additionally, in one donor the genomic DNA encoding the VH and CH1 domains was deleted,

103 mosome (BAC) reporters using human and mouse genomic DNAs encompassing the TERT genes and neighboring

104 Here we describe the first genomic DNA expression library generated using the high-

105 In contrast, genomic DNA expression vectors generate physiologically-

106 red to those of the other three assays using genomic DNA extracted from 40 adultDermacentor variabili

107 ratio method to determine telomere length in genomic DNA extracted from buccal smears from 63 patient

108 It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clin

109 hole-exome sequencing (WES) was performed on genomic DNA extracted from fresh-frozen whole blood and

110 Fluorescence titrations using genomic DNA extracted from various cell lines demonstrat

111 Existing yeast genomic DNA extraction methods are not ideally suited to

112 The technique involves fragmentation of genomic DNA followed by adapter ligation, bisulfite conv

113 for this purpose, which utilizes shearing of genomic DNA followed by specific PCR amplification of tr

114 d with various concentrations of Aspergillus genomic DNA for extraction, following international reco

115 ocking of MGE transmission through releasing genomic DNA for homologous recombination while simultane

116 100 cross-linked cells for ChIP or 500 pg of genomic DNA for MeDIP (compared to 10(6)-10(7) cells for

117 he RSS that facilitates accessibility of the genomic DNA for the RAG recombinase.

118 Large genomic DNA fragment (up to 95 kb) deletion mice were ge

119 s observation, we combined CRISPR/Cas9-based genomic-DNA-fragment editing with chromosome-conformatio

120 pproaches, microfluidic partitioning of long genomic DNA fragments and barcoding of shorter fragments

121 Enrichment for Sequence Analysis), isolates genomic DNA fragments in microfluidic droplets and perfo

122 newly designed primers were evaluated using genomic DNA from 11Rickettsiaspecies belonging to the sp

123 We collected clinical data and genomic DNA from 148 of 188 consecutive patients diagnos

124 we performed mate pair sequencing (MPseq) on genomic DNA from 24 PDAC tumors, including 15 laser-capt

125 Using genomic DNA from a homogeneous cohort of 125 AML patient

126 onal signatures are indeed present in tumour genomic DNA from a variety of cancer types.

127 Genomic DNA from all 1007 NYBCS probands was sequenced f

128 DNA libraries are constructed using native genomic DNA from any source of interest, preserving cell

129 culties in obtaining sufficient, highly pure genomic DNA from clinical specimens.

130 equence enrichment across 250 kb of targeted genomic DNA from five unique genomic loci.

131 In this study, we amplified genomic DNA from individual hippocampal neurons using th

132 lective amplification of Wolbachia pipientis genomic DNA from infected Drosophila melanogaster and My

133 Samples contained equimolar mixtures of genomic DNA from lambda bacteriophage, Escherichia coli

134 n was evaluated by quantitative PCR assay of genomic DNA from liver and spleen.

135 non-pregnant and pregnant female donors and genomic DNA from maternal buffy coat and placenta sample

136 oned from the patient as well as in cDNA and genomic DNA from other individuals, suggesting that gene

137 We performed whole-exome sequencing of genomic DNA from patients with familial CIPO syndrome.

138 The concept was proved on analysis of the genomic DNA from PC-3 cells and DNA isolated from melano

139 We analyzed genomic DNA from skin fibroblasts using whole-exome sequ

140 Genomic DNA from T. thermophila strain EPR85 contains ge

141 The folding of genomic DNA from the beads-on-a-string-like structure of

142 Sequencing of genomic DNA from the infant's saliva yielded 1.44 Gbp of

143 t AID is transiently in spatial contact with genomic DNA from the time the nuclear membrane breaks do

144 and 185 adult controls were genotyped using genomic DNA from venous blood.

145 idium difficile genome requires high-quality genomic DNA (gDNA) as the starting material.

146 frequency of insertions was compared between genomic DNA (gDNA) collected from cells in the biofilm a

147 rmore, the technique can successfully target genomic DNA (gDNA) fragments of length >500 bp, and it c

148 uNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and

149 We obtained 866 genomic DNA (gDNA) sequences from thirteen individuals a

150 owever, inclusion of noncoding components of genomic DNA (gDNA) such as introns may help promote more

151 When genomic DNA (gDNA) was extracted using two commercial DN

152 NA) of specific pathogen species relative to genomic-DNA (gDNA) of the same species (P:G ratios) are

153 nt amplification and, together with maternal genomic DNA, genotyped for approximately 300,000 single-

154 e with more complex materials, such as human genomic DNA, has been less studied.

155 rchitecture using purified components: yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and

156 Papa-B exon 2 and 3 were determined from the genomic DNA in 255 fecal samples, minimally representing

157 ention for their ability to precisely modify genomic DNA in a programmable manner.

158 ction methods to unspecifically process long genomic DNA in a short time frame, e.g. for pathogen det

159 lian CD59 genes to identify the only span of genomic DNA in C. porcellus that is homologous to a port

160 response to ribonucleotide incorporation in genomic DNA in human cells.

161 hibitor also prevented the cleavage of viral genomic DNA in infected cells.

162 de 5hmC patterns from as little as 500 ng of genomic DNA in less than 4 days.

163 However, massive loss of genomic DNA in these lineages often occurred in the dist

164 Finally, analysis of genomic DNA in WT mice revealed cisplatin-induced hypome

165 lective isolation of a gene of interest from genomic DNA in yeast followed by its direct transfer to

166 MCL-1 association with genomic DNA increased postirradiation, and the protein c

167 While enzymatic restriction of human genomic DNA increases accessibility for some assays, in

168 The assay requires just picogram levels of genomic DNA input, is sensitive and specific enough to d

169 RAG1/2 can also induce genomic DNA insertions by transposition and trans-V(D)J

170 In eukaryotes, the packaging of genomic DNA into chromatin plays a critical role in gene

171 We confirmed that RAG1/2 also mobilizes genomic DNA into independent physiological breaks by ide

172 ent of MIF to the nucleus, where MIF cleaves genomic DNA into large fragments.

173 oiling and conversion of multiple regions of genomic DNA into left-handed Z-form.

174 The packaging of genomic DNA into nucleosomes creates a barrier to transc

175 Wrapping of genomic DNA into nucleosomes poses thermodynamic and kin

176 virus terminase complex cleaves concatemeric genomic DNA into unit lengths during genome packaging an

177 Prokaryotic and eukaryotic genomic DNA is comprised of the four building blocks A,

178 Bound genomic DNA is eluted from the TF and sequenced using ne

179 The accurate and complete replication of genomic DNA is essential for all life.

180 HBoV genomic DNA is frequently detected in both ill and healt

181 This is because genomic DNA is physically associated with an astonishing

182 ient (Mili(-/-)) mice demonstrate that brain genomic DNA is preferentially hypomethylated within inte

183 or the understanding of how accessibility to genomic DNA is regulated in cells.

184 Chromatinized genomic DNA is resistant to MjAgo degradation, and recom

185 rough a vast excess of competing, unmodified genomic DNA, is a mechanistic challenge that may limit t

186 We performed bisulfite sequencing PCR of genomic DNA isolated from HBV-related HCCs and HBV repli

187 We quantified total human genomic DNA isolated from plasma samples for 503 patient

188 is protocol has been successfully applied to genomic DNA isolated from primary cell culture, sorted c

189 They conducted quantitative real-time PCR on genomic DNA isolated from tumor and matched normal biops

190 alable single-step approach performed at the genomic DNA level in solution that combines with most do

191 over, lambda-exo digestion of nonreplicating genomic DNA (LexoG0) enriches GC-rich DNA and G4 motifs

192 ied TFs with next-generation sequencing of a genomic DNA library.

193 els of gene expression by carrying the whole genomic DNA locus of a gene including its regulatory ele

194 Our results suggest coincidence of massive genomic DNA losses and increased power of genetic drift,

195 sitive colonies derived from Cryptosporidium genomic DNA, </=25%).

196 ous generation of interstrand cross-links in genomic DNA may contribute to aging, neurodegeneration,

197 Hence, A-tracts embedded in genomic DNA may or may not interact preferentially with

198 ent discovery on how double-strand breaks of genomic DNA mediate the rapid induction of activity-depe

199 H2 mutant expression caused both histone and genomic DNA methylation changes that can be reversed whe

200 del is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth p

201 Genomic DNA methylation maps (methylomes) encode genetic

202 The epigenetic information encoded in the genomic DNA methylation pattern is translated by methylc

203 We compared genomic DNA methylation patterns and gene expression in

204 To gain insight into how genomic DNA methylation patterns are regulated during iA

205 -(+)-fipronil dysregulated a higher level of genomic DNA methylation than R-(-)-fipronil.

206 eported factor whose loss leads to a gain in genomic DNA methylation.

207 emonstrate in this study that IL-26 binds to genomic DNA, mitochondrial DNA, and neutrophil extracell

208 TEX also deliver genomic DNA, mRNA, and microRNAs to immune cells, thereb

209 We used cheek swabs to obtain the genomic DNA of 200 ADHD male probands (mean age: 8.7 yea

210 ral modified bases have been observed in the genomic DNA of bacteriophages, prokaryotes, and eukaryot

211 formation of O(4)-POBdT in naked DNA and in genomic DNA of cultured mammalian cells exposed with NNK

212 deoxy-5-(hydroxymethyl)uridine (5hmU) in the genomic DNA of Escherichia coli.

213 ymethylcytosine, and 5-formylcytosine in the genomic DNA of human CMSCs isolated from diabetic donors

214 We compared DNA-me profiles in genomic DNA of whole blood (WB) isolated at EDIC Study b

215 In this cohort study, genomic DNA of women from 12 major cancer centers with a

216 provirus gene-editing were confirmed by cell genomic DNA PCR and fluorescent marker expression analys

217 g a limit of detection of 40 copies of human genomic DNA per reaction volume.

218 using a specific 16S ribosomal RNA probe and genomic DNA probe.

219 hermore, high-throughput sequencing of human genomic DNA pulled down by the biotin-labeled tandem tet

220 DNA sequences, linear or circular DNA, bulk genomic DNA, recombinant or native Drosophila core histo

221 efficiently locate sequence homology across genomic DNA remains unclear.

222 s ribonucleoside monophosphates (rNMPs) from genomic DNA, replacing the error with the appropriate de

223 Genomic DNA replication requires helicases to processive

224 for cellular resistance to etoposide during genomic DNA replication.

225 s of detection were 0.01% and 0.01 ng/mul of genomic DNA, respectively.

226 Transfection of human PBMC's with TTSuV1 genomic DNA resulted in productive viral infection which

227 alysis of mutated target sequences and human genomic DNA reveal that Cascade recognizes an extended p

228 New approaches for genomic DNA/RNA detection are in high demand in order to

229 rder, we performed whole-exome sequencing of genomic DNA samples from both individuals.

230 d wnt7b, estimated from direct sequencing of genomic DNA samples of injected lamprey larvae, were 68/

231 Then the biosensor was applied to genomic DNA samples, extracted from human lymphocytes an

232 assay to be used in the detection of SNPs in genomic DNA samples.

233 E), chromatin immunoprecipitation (ChIP) and genomic DNA-seq.

234 inder model without fitting parameters, with genomic DNA sequence being the only input, we further va

235 The data show that the 20 bp-long genomic DNA sequence is necessary for RHO expression and

236 iple generations in the presence of the same genomic DNA sequence.

237 Spectra were directly compared with genomic DNA sequences (internal transcribed spacer, ITS)

238 PCR method in diagnostics, we amplify human genomic DNA sequences from a approximately 1 muL droplet

239 After retrieving all genomic DNA sequences from the NCBI GenBank, over 1 x 10

240 fficient program to predict SINE elements in genomic DNA sequences.

241 that could not be predicted on the basis of genomic DNA sequencing alone.

242 Here, we employed genomic DNA sequencing of multiple variable (V) regions

243 Genomic DNA sequencing technologies have been one of the

244 n of metagenomics through massively parallel genomic DNA sequencing.

245 Overall, our findings indicate that genomic DNA serves as a reservoir to initiate a pro-infl

246 iments using this method to sequence E. coli genomic DNA showed that the TGIRT enzyme has surprisingl

247 ant nucleoid-associated proteins at numerous genomic DNA sites and stabilization of distinct long-ran

248 t electrophoretic processing of unfragmented genomic DNA strands is time-consuming, because of the le

249 controlled generation of long single-strand genomic DNA substrates, we show that the mutation signat

250 scriptional factories with replicating viral genomic DNA suggests that KSHV assembles an "all-in-one"

251 Large-scale sequencing of prokaryotic (meta)genomic DNA suggests that most bacterial natural product

252 gulation in the DNA polymerase choice during genomic DNA synthesis.

253 otype information using up to 100 times less genomic DNA than some methods and enables the accurate d

254 ic marker is randomly transposed in vitro in genomic DNA that is then used for natural transformation

255 Abasic (Ap) sites are common lesions in genomic DNA that readily undergo spontaneous and amine-c

256 Genedrive PCR amplification was tested with genomic DNA; the performance of the complete (sample pro

257 ine dimers (the major UV-induced lesions) in genomic DNA; the quantum yield of these dimers in TEL21/

258 ng suggests that the protein associates with genomic DNA through this interaction.

259 stranded DNA molecules to invade and replace genomic DNA through two joint illegitimate recombination

260 SMASH utilizes random fragmentation of input genomic DNA to create chimeric sequence reads, from whic

261 However, transcription also sensitises genomic DNA to damage from a number of endogenous source

262 and single molecule real-time sequencing on genomic DNA to determine these target sequences and thei

263 We amplified switch regions from genomic DNA to investigate the quality of the double-str

264 will enable the mapping of 5-fU and 5-hmU in genomic DNA, to provide insights into their functional r

265 Genomic DNA, total RNA, and protein were isolated from m

266 ents from multiple PCR assays, on four human genomic DNAs treated with four endonuclease restriction

267 hly mutagenic DNA adduct that forms in human genomic DNA upon reaction with methylating agents of die

268 Because genomic DNA used in DAP-seq retains 5-methylcytosines, w

269 We verified the presence of the mutations in genomic DNA using a combination of molecular inversion p

270 he physical separation of polyA(+) mRNA from genomic DNA using a modified oligo-dT bead capture and t

271 on gene expression by parallel processing of genomic DNA using bisulfite and oxidative bisulfite conv

272 junctions are cloned directly from isolated genomic DNA using LAM-PCR and unidirectionally ligated t

273 te whole-genome amplification of single-cell genomic DNA using linear nucleic acid amplification.

274 expansion number in the Huntington's gene of genomic DNA using quantitative PCR.

275 ed information is available on the amount of genomic DNA variation between HSV-2 strains because only

276 horylation state in complete (containing the genomic DNA) versus empty (genome-free) HBV virions.

277 AP) sites are generated at high frequency in genomic DNA via spontaneous hydrolytic, damage-induced o

278 ues were collected 14 months after delivery; genomic DNA was analyzed by PCR to detect the Dnajb1-Prk

279 Patients and Methods Genomic DNA was analyzed for TP53, CTNNB1, CDKN1C, ATRX,

280 The genomic DNA was captured with the Agilent SureSelect Hum

281 The extracted genomic DNA was detected based on changes in the charge

282 Genomic DNA was extracted from blood samples collected f

283 Genomic DNA was extracted from brain tissue in all cases

284 Genomic DNA was isolated from bone marrow collected at r

285 re collected from each study participant and genomic DNA was isolated.

286 Genomic DNA was obtained from 1,460 participants, aged 3

287 Genomic DNA was tested for the KRAS-variant in a CLIA-ce

288 WGS from blood-derived genomic DNA was used for homozygosity mapping and a rare

289 The isolation and enrichment of genomic DNA were achieved by a combination of three oocy

290 be a consequence of Brownian movement of the genomic DNA, which would be rectified and harnessed by t

291 isolating and lysing single cells, digesting genomic DNA with a methylation-sensitive restriction end

292 g a large fraction or all of a nucleoside in genomic DNA with a modified nucleoside.

293 Here, we demonstrate that pre-treatment of genomic DNA with CRISPR-Cas9 nucleases to generate doubl

294 ding site discovery method that interrogates genomic DNA with in-vitro-expressed TFs.

295 m for efficient, programmable replacement of genomic DNA with long (>100-kb) synthetic DNA, through t

296 3 are viable and able to replicate undamaged genomic DNA with normal kinetics.

297 triphosphates (rNTPs) are incorporated into genomic DNA with relatively high frequency during normal

298 ing DNA methylation involve the treatment of genomic DNA with sodium bisulfite; however, this method

299 allows stable labeling and monitoring of HIV genomic DNA within infected cells during cytoplasmic tra

300 GA from 10(6) cells or approximately 1 ng of genomic DNA yielded high-quality coverage detecting near