ライフサイエンスコーパス: geranylgeranylated

1 L, a mutant construct of Rheb designed to be geranylgeranylated.

2 t the enzyme is only farnesylated and is not geranylgeranylated.

3 Ras-GRF2 when it was farnesylated instead of geranylgeranylated.

4 GTase:GGPP complex revealed that Rab is mono-geranylgeranylated.

5 d only Ras-CIIS and not Ras-CII(M,L), and it geranylgeranylated all three substrates as well or bette

6 ed after removal of methionine, C-terminally geranylgeranylated and carboxymethylated with removal of

7 small GTPases RalA and RalB are exclusively geranylgeranylated and that inhibition of their geranylg

8 form of oncogenic H-Ras that is exclusively geranylgeranylated and whose processing is resistant to

9 cysteine 192 RhoB mutant is farnesylated and geranylgeranylated as efficiently as wild type RhoB.

10 eir native form, most Rab GTPases are doubly geranylgeranylated at the C terminus to achieve localiza

11 ) is able to hydrolyze the methyl ester of a geranylgeranylated beta gamma isoform (beta 1 gamma 2).

12 effect (less than 2-fold) on the ability of geranylgeranylated beta gamma to activate phosphatidylin

13 inal cysteines and potentially can be double-geranylgeranylated by heterodimeric Rab geranylgeranyltr

14 These 22-26-kDa proteins were shown to be geranylgeranylated by labelling J774 cells with [(3)H]ge

15 s XXCC in Rab1a) in which both cysteines are geranylgeranylated by Rab GG transferase (RabGGTase).

16 b1B effector-domain mutant (D44N) that, when geranylgeranylated by Rab:geranylgeranyltransferase (GGT

17 orientation seems to depend primarily on the geranylgeranylated C-terminus of Ggamma2 along with basi

18 GGTI-DU40 blocks prenylation of a number of geranylgeranylated CaaX proteins.

19 e amino acid sequences located upstream from geranylgeranylated cysteine residues in the C-terminal t

20 s, is more active with the farnesylated than geranylgeranylated cysteinyl substrate.

21 e low micromolar range is found with various geranylgeranylated/farnesylated analogues.

22 The current data indicate that geranylgeranylated FBL2 binds to NS5A in a reaction cruc

23 ted CaaX peptides including farnesylated and geranylgeranylated forms of human Ha-Ras, Ki-Ras, N-Ras,

24 YopT also cleaves both farnesylated and geranylgeranylated forms of RhoA.

25 is issue is complex because farnesylated and geranylgeranylated forms of RhoB (RhoB-F and RhoB-GG) bo

26 Geranylgeranylated Gbetagamma, but not a soluble mutant

27 RhoB is both farnesylated (F) and geranylgeranylated (GG), and RhoB-F has been suggested a

28 alization of farnesylated Ggamma(1), but not geranylgeranylated Ggamma(2), required carboxyl methylat

29 that both recombinant farnesylated GRK1 and geranylgeranylated GRK7 co-precipitate with a glutathion

30 now available, the role of methylation for a geranylgeranylated heterotrimeric G protein may be addre

31 otein, the data suggest the involvement of a geranylgeranylated host protein in HCV replication.

32 n, and bioinformatic search, we identified a geranylgeranylated host protein required for HCV RNA rep

33 s (HCV) RNA replication requires one or more geranylgeranylated host proteins.

34 arnesylation is inhibited and Ki-Ras becomes geranylgeranylated in a dose dependent manner.

35 Ki-Ras4B, but not other Ras isoforms, can be geranylgeranylated in vitro by FPT.

36 came less responsive to Ras-GRF2 when it was geranylgeranylated instead of farnesylated.

37 esylated, whereas the 22-28-kDa proteins are geranylgeranylated, irrespective of the labeling prenol.

38 se studies have established that gain of the geranylgeranylated isoform of the small GTPase RhoB is e

39 Because K-Ras can be both farnesylated and geranylgeranylated it is not known whether both farnesyl

40 distinct functions for farnesylated and for geranylgeranylated K-Ras, which is generated when farnes

41 ss farnesylated Ha-Ras, N-Ras, and Ki-Ras or geranylgeranylated Ki-Ras.

42 a mixture of unprocessed, farnesylated, and geranylgeranylated-Ki4B-Ras in cells treated with microm

43 PSN-1 cells, a mixture of farnesylated- and geranylgeranylated-Ki4B-Ras in cells treated with nanomo

44 IL-4 production, suggesting novel roles for geranylgeranylated (lovastatin-sensitive, BZA-5B-insensi

45 ets will lack both polybasic and potentially geranylgeranylated methionine-CAAX motifs.

46 rm II suggests the potential binding mode of geranylgeranylated-methylated KRAS4b to PDEdelta.

47 A rhodamine-labeled geranylgeranylated/methylated cysteine derivative is use

48 Expression of the geranylgeranylated mutant form of a novel farnesylated s

49 Expression of a geranylgeranylated mutant form, Ras1-CVIL, which can byp

50 Rab proteins are geranylgeranylated on one or two C-terminal cysteines by

51 A geranylgeranylated peptide corresponding to the C-termin

52 rt we identify Rap1, not RhoA, as a critical geranylgeranylated protein mediating phorbol ester-stimu

53 To identify the geranylgeranylated protein(s) involved in p21(WAF1/CIP1)

54 iates IL-1beta induction of NOS-2, whereas a geranylgeranylated protein(s) represses this induction.

55 a farnesylated protein, such as Rheb, and a geranylgeranylated protein, such as Rho, both of which h

56 s the HCV genome does not encode a canonical geranylgeranylated protein, the data suggest the involve

57 rol potentiator of reductase regulation is a geranylgeranylated protein.

58 on and signaling activities of the wild-type geranylgeranylated proteins and that Ral GTPases do not

59 The data demonstrate that geranylgeranylated proteins are critical in H-Ras oncoge

60 These results demonstrate that geranylgeranylated proteins are required for cells to pr

61 so produced recognized both farnesylated and geranylgeranylated proteins as substrates, including far

62 orting cell proliferation, in the absence of geranylgeranylated proteins like RhoA.

63 elative contributions of farnesylated and/or geranylgeranylated proteins on cell cycle progression fr

64 The processing of the geranylgeranylated proteins RhoA, Rap1, and R-Ras, but n

65 However, the geranylgeranylated proteins that are targeted by GGTIs t

66 ased on the C-terminal CAAL sequence of many geranylgeranylated proteins.

67 factor, Yip1p that specifically binds the di-geranylgeranylated Rab and does not interact with mono-p

68 and adipocytes and increases the amounts of geranylgeranylated Rab-3 and Rab-4 proteins.

69 time, insulin also increased the amounts of geranylgeranylated Rab-3 in 3T3-L1 fibroblasts from 44 +

70 adipocytes, insulin increased the amounts of geranylgeranylated Rab-4 from 38 +/- 0.6% in control cel

71 affinity beads after incubating recombinant geranylgeranylated Rab1B with FLAG epitope-tagged GDI in

72 The geranylgeranylated Rabs regulate vesicular movement by o

73 amounts of completely unprocessed Rap 1A and geranylgeranylated Rap 1A; the lack of Rap 1A processing

74 Rnd1, Rnd2, and geranylgeranylated Rap1A interact similarly with 14-3-3.

75 rnesylated Ggamma1 subunit of transducin and geranylgeranylated Rap1B, was also blocked.

76 reading, and secretion, previously linked to geranylgeranylated Rho and Rab family members.

77 es, it could be concluded that impairment of geranylgeranylated Rho and Rac GTPase function is most l

78 rated decreased membrane localization of the geranylgeranylated Rho family members (RhoA, Cdc42, and

79 contrast, the intracellular localizations of geranylgeranylated Rho GTPases were not perturbed.

80 C3 exotoxin, which specifically inactivates geranylgeranylated Rho GTPases, mimicked the effect of s

81 sferase I (GGTI-I), increased the amounts of geranylgeranylated Rho-A and potentiated the transactiva

82 activity 3-fold and augmented the amounts of geranylgeranylated Rho-A by 18%.

83 rase (GGTase) I and increased the amounts of geranylgeranylated Rho-A from 47% to 60% (P:<0.05).

84 activates GGTase I, increases the amounts of geranylgeranylated Rho-A protein, and potentiates the Rh

85 insulin-induced increases in the amounts of geranylgeranylated Rho-A resulted in potentiation of the

86 insulin to increase the cellular amounts of geranylgeranylated Rho-A results in potentiation of the

87 activity of GGTase I and the availability of geranylgeranylated Rho-A.

88 indered these T cell responses by decreasing geranylgeranylated RhoA and farnesylated Ras at the plas

89 Selective inhibition of geranylgeranylated RhoA-associated kinase replicated the

90 it a loss of farnesylated RhoB but a gain of geranylgeranylated RhoB (RhoB-GG), which is associated w

91 FTI treatment rapidly elevates the level of geranylgeranylated RhoB in cells and that this event is

92 FTI treatment elicits a gain of the geranylgeranylated RhoB isoform (RhoB-GG) that occurs du

93 Ectopic expression of the geranylgeranylated RhoB isoform elicited in cells by FTI

94 2 through calcium-mediated activation of the geranylgeranylated small G protein Rap1 and the Rap1 ass

95 Based on a structural analogy between geranylgeranylated SNAREs and the GPI-HA mutant influenz

96 contributions of C15 (farnesylated) and C20 (geranylgeranylated) species.

97 with nonprenylated GTPases that will become geranylgeranylated than with nonprenylated GTPases that

98 ate pathway, and G protein Ggamma1, which is geranylgeranylated, thus representing an end point of is

99 GDP-bound Rab is geranylgeranylated with 10-50-fold higher affinity than