ライフサイエンスコーパス: gliadin

1 hat recognized epitopes from gamma-and omega-gliadin.

2 samples at levels comparable with those for gliadin.

3 educed in the presence of partially digested gliadin.

4 -cell factors in driving mucosal response to gliadin.

5 e of a peptic-tryptic digest (P-T digest) of gliadin.

6 iac disease from the accidental ingestion of gliadin.

7 GS, and omega-gliadins over alpha- and gamma-gliadins.

8 8(+) T cell epitopes mapped within the gamma-gliadins.

9 precipitated glutenins more efficiently than gliadins.

10 surface hydrophobicity of glutenins, but not gliadins.

11 A synthetic gene coding for gamma-gliadin 1 (GG1) was expressed in Escherichia coli.

12 Recombinant gamma-gliadin 1 (rGG1) was purified and characterized biochemi

13 e NC-IBS group, 6.51% had antibodies against gliadin, 1.22% against tissue transglutaminase, and 0.61

14 k III as very low molecular weight monomeric gliadins (10-28kDa), peak IV and V, collectively, as alb

15 signified glutenins (30-130kDa), peak II as gliadins (20-55kDa), peak III as very low molecular weig

16 din binds to CXCR3 and that at least 2 alpha-gliadin 20mer synthetic peptides are involved in this bi

17 liac patients by the detection of gluten and gliadin 33-mer equivalent peptidic epitopes (33EPs) in h

18 A model peptide sequenced in alpha-gliadin, 33-mer (LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF), was

19 complexes and intestinal permeability to the gliadin 3H-p31-49 peptide were analyzed in polarized mon

20 Our data also suggest that alpha-II-gliadin 62-70 is a DM-suppressed epitope.

21 Levels of IgG directed at gliadin (a component of gluten) and casein (a milk prote

22 ty (ROA) spectra of the wheat proteins alpha-gliadin (A-gliadin), omega-gliadin, and a 30 kDa peptide

23 ized by intestinal inflammation triggered by gliadin, a component of dietary gluten.

24 an immune-mediated enteropathy triggered by gliadin, a component of the grain protein gluten.

25 We investigated whether gliadin, a protein present in wheat flour, could activat

26 Gliadins, a fraction of gluten proteins, are the main co

27 nvestigate the immune reactivity of the anti-gliadin Ab response toward neural proteins.

28 erized the binding of affinity-purified anti-gliadin Abs from immunized animals to brain proteins by

29 Anti-gliadin Abs from patients with celiac disease also bound

30 Alpha-gliadin affinity column was loaded with intestinal mucos

31 c disease were immunoglobulins, IgA/IgG anti-gliadin (AGA), and endomysial antibodies (EMA).

32 After gluten gavage, gliadin amount and proteolytic activities were measured

33 leukocyte antigen type and detection of anti-gliadin and anti-transglutaminase 2 antibodies to identi

34 ave a significant role in the degradation of gliadin and antigen production.

35 Our results suggest that gliadin and beta-glucan stimulate IL-23 secretion throug

36 T cell lines at levels similar to those for gliadin and caused secretion of cytokines from cultured

37 results suggest that the duplicates of alpha-gliadin and GLR genes have likely taken different evolut

38 Known gliadin and glutenin sequences were largely determined t

39 aricain appears to be capable of detoxifying gliadin and has the potential to mitigate the problems c

40 y using murine monoclonal antibodies against gliadin and high-molecular-weight glutenin subunits.

41 ) or 2-propanol (75 degrees C) decreased the gliadin and increased the glutenin content.

42 We also demonstrated that ID331 omega-gliadin and its derived peptide omega(105-123) exerted a

43 Gliadin and its peptide derivatives, 33-mer and p31-43,

44 Moreover, MC reactivity to gliadin and its peptides was characterized by using in v

45 turn, enables paracellular translocation of gliadin and its subsequent interaction with macrophages

46 Marker 1 was identified as omega1,2-gliadin and markers 2, 3a and 3b were identified as gamm

47 ity resulting from molecular mimicry between gliadin and nervous system proteins has been proposed to

48 icain as a means of reducing the toxicity of gliadin and open the way for enzyme therapy as an adjunc

49 emolina gluten proteins consist of monomeric gliadin and polymeric glutenin and determine the quality

50 The crucial epitopes that confer toxicity to gliadin and related prolamins continue to be defined, as

51 that is induced by dietary exposure to wheat gliadin and related proteins from barley, rye, and possi

52 ted disorder triggered by ingestion of wheat gliadin and related proteins in genetically susceptible

53 tinct cluster containing the wheat protein A-gliadin and the plant viruses potato virus X, narcissus

54 In comparison, omega-gliadin and the T-A-1 peptide were found to consist of l

55 d generate an antibody response against both gliadin and tTGase.

56 to study the interaction of resveratrol with gliadin and zein.

57 ectants were performed to confirm binding of gliadin and/or 26 overlapping 20mer alpha-gliadin synthe

58 Gliadins and A-gliadin peptide P31-43, in particular, ac

59 everal protein classes, such as LTPs, omega5-gliadins and alpha-amylase/trypsin inhibitors.

60 IgE binding to deamidated gamma- and omega2-gliadins and deamidated total gliadins, frequently with

61 -secalins were most reactive, whereas omega5-gliadins and gamma-, B- and D-hordeins were detected wit

62 Overall, omega1,2-gliadins and gamma-75k-secalins were most reactive, wher

63 branch that existed separate from the gamma-gliadins and gamma-hordeins in an ancestral Triticeae pr

64 ic analysis shows that the orthologous delta-gliadins and gamma3-hordeins form a distinct prolamin br

65 The gliadins and glutenins were also reduced in vivo during

66 e interaction of the environmental triggers (gliadins and glutenins) with these gene products to trig

67 eaved the major indigenous storage proteins, gliadins and glutenins, after they too had been reduced,

68 Gliadins and glutenins, the major storage proteins of wh

69 c activity and electrophoretic properties of gliadins and glutenins.

70 mainly accumulate in storage proteins called gliadins and glutenins.

71 ponsible for transcriptional derepression of gliadins and low-molecular-weight glutenins (LMWgs) by a

72 tides (a 34-mer and a 26-mer) found in omega-gliadins and low-molecular-weight glutenins that had bee

73 at proteins alpha-gliadin (A-gliadin), omega-gliadin, and a 30 kDa peptide called T-A-1 from the high

74 rthered the isolation of the epitopes within gliadin, and other related proteins, that are critical f

75 e addition of DQ8 contributes sensitivity to gliadin, and the addition of the NOD background contribu

76 tissue transglutaminase (tTG) and deamidated gliadin, and the classifier accuracy was independent of

77 the three patients lacking IgE to omega(5) -gliadin, and to HMW glutenin in 59%.

78 nd PFPEQxFP that identified omega- and gamma-gliadins, and their deamidated forms, as immunodominant

79 non-celiac gluten sensitivity patients anti-gliadin antibodies IgG persistence after gluten withdraw

80 Anti-gliadin antibodies of both IgG and IgA classes were assa

81 nts (93.2%) showed the disappearance of anti-gliadin antibodies of IgG class after 6 months of gluten

82 h a high prevalence of first generation anti-gliadin antibodies of IgG class has been reported in thi

83 e the effect of the gluten-free diet on anti-gliadin antibodies of IgG class in patients with non-cel

84 Anti-gliadin antibodies of the IgG class disappear in patient

85 Regarding IgA gliadin antibody determination, sensitivity improved fro

86 gh serum immunoglobulin A (IgA) and IgG anti-gliadin antibody titers and typical human lymphocyte ant

87 IgE antibodies to recombinant omega(5) -gliadin are detectable in a majority of WDEIA patients,

88 t human monoclonal antibodies (hmAb) against gliadin are produced by cloning antibody genes from sing

89 The expressed delta-gliadins are encoded by a single gene in each of the hex

90 mins important for its end-use traits, alpha-gliadins are the most abundant, and are also a major cau

91 Gluten proteins, namely gliadins, are the primary trigger of the abnormal immune

92 he immunodominant apolar peptide from alpha2-gliadin as a target for selection.

93 The microarray revealed specifically gamma-gliadin as the second most important allergen.

94 found throughout the immunogenic regions of gliadin, as well as in homologous proteins in barley and

95 inding constant was higher for zein than for gliadin at 35 degrees C.

96 have better diagnostic accuracy over native gliadin-based tests.

97 chaete scute homologous protein (DQ2-alpha-I-gliadin; BestFit quality 41 versus 22 for random).

98 ynamic parameters suggested that resveratrol-gliadin binding mainly occurs through hydrophobic intera

99 al membrane lysates to identify the putative gliadin-binding moiety.

100 Gliadin binds to CXCR3 and leads to MyD88-dependent zonu

101 n and colocalization experiments showed that gliadin binds to CXCR3 and that at least 2 alpha-gliadin

102 ptide is resistant to further digestion of a gliadin by intestinal brush border enzymes and is a high

103 arkers 2, 3a and 3b were identified as gamma-gliadins by means of N-terminal sequence analysis and de

104 adults for whom the specific IgE to omega-5 gliadin can be a useful diagnostic test.

105 Therefore, an alpha-gliadin cDNA screen of human cDNA and genomic libraries

106 We also investigated the effect of gliadin complex formation in intestinal biopsy specimens

107 We measured the effects of gliadin complex formation on cytokine release ex vivo us

108 ansport of secretory immunoglobulin A (SIgA)-gliadin complexes.

109 be focused on a limited region of the alpha gliadin component of gluten, and previous studies have s

110 The number of potential disease-triggering gliadin components remains large.

111 Gliadins contain alpha/beta-, gamma- and omega-types whe

112 susceptible individuals by the ingestion of gliadin-containing grains.

113 w that, under the conditions investigated, A-gliadin contains a considerable amount of hydrated alpha

114 ed assay can be used to quickly estimate the gliadin content in foods in order to protect people with

115 e was found to be more effective in reducing gliadin content than the crude papain and the resultant

116 shown to specifically reduce the immunogenic gliadin content, in gastric conditions, to below the thr

117 ith the amount of toxic epitopes: omega-type gliadins content explain about 40% of the variation of t

118 obtained after in vitro digestion of a wheat gliadins crude extract and further characterized by LC-E

119 Gliadin-CXCR3 interaction was further analyzed by immuno

120 Triticum aestivum gliadin derived peptides were employed as a positive con

121 e is directed toward an immunodominant alpha-gliadin-derived peptide (DQ8-glia-alpha1).

122 logical effects of ID331 Triticum monococcum gliadin-derived peptides in human Caco-2 intestinal epit

123 For patients with celiac disease, gliadin detoxification via the use of gliadinases may pr

124 Our findings confirmed that ID331 gliadin did not enhance permeability and did not induce

125 ured potential open reading frames with high gliadin domain II and domain IV homologies (BestFit qual

126 ear striking similarity to two or more wheat gliadin domains raised the possibility that human intest

127 positive CD serologic test results (IgA/IgG gliadin, endomysium, or tissue transglutaminase) and com

128 redesign the active site of the acid-active gliadin endopeptidase KumaMax.

129 pe homology, particularly to the DQ2-gamma-I-gliadin epitope (i.e. TFIIA, FOXJ2 and IgD; mean BestFit

130 ll surface, and the presentation of alpha-II-gliadin epitope (residues 62-70) to murine, DQ2-restrict

131 h, in the presence of either immunodominant gliadin epitopes (p(alpha)-2 and p(alpha)-9) or a non-im

132 ted binding to HLA molecules for identifying gliadin epitopes and demonstrate that HLA class I molecu

133 Specific gliadin epitopes are being analyzed.

134 innate and adaptive immune responses to key gliadin epitopes are now both recognized to be important

135 Specific gliadin epitopes have been defined using T-cell response

136 g the in situ T-cell recognition of dominant gliadin epitopes.

137 r 3 h, before incubation with immunodominant gliadin epitopes.

138 ens tissue transglutaminase-2 and deamidated gliadin, exhibiting 71% sensitivity and 99% specificity

139 city of 92.9%, which was higher than that of gliadin extract (e).

140 he best characterized environmental factors (gliadin) for any common autoimmune disease.

141 capable of degrading >99% of the immunogenic gliadin fraction in laboratory-simulated gastric digesti

142 of the small intestinal mucosa caused by the gliadin fraction of wheat gluten and similar alcohol-sol

143 Wheat, specifically its omega-5 gliadin fraction, is the most common allergen implicated

144 showed that the chromatograms of the reduced gliadin fractions were most suitable for the distinction

145 A gliadin fragment can activate the innate immune system,

146 und to directly respond to nonimmunodominant gliadin fragments by releasing proinflammatory mediators

147 ma- and omega2-gliadins and deamidated total gliadins, frequently with high concentrations.

148 Samples of hydrolyzed gliadin from wheat and a barley beer were used.

149 vation by peptic and tryptic (PT) digests of gliadins from 2 monococcum lines.

150 amples before and after microwave treatment, gliadins from treated flours showed significantly reduce

151 immunodetection with specific antibodies for gliadins, gamma-gliadins, LMW subunits and antigenic epi

152 This single delta-gliadin/gamma3-hordein ortholog may be a general feature

153 b region from Ae. tauschii locates the delta-gliadin gene to the complex Gli-1 plus Glu-3 region on c

154 r the detection of T. aestivum, based on the gliadin gene, is inadequate.

155 which have given rise to 12 copies of alpha-gliadin genes clustered within a 550-kb region.

156 cies estimated that between 25 and 150 alpha-gliadin genes reside in the Gli-2 locus regions.

157 sequences also allow assignment of the delta-gliadin genes to the A and D genomes, respectively, with

158 tionary relationship of the duplicated alpha-gliadin genes were obtained from their genomic organizat

159 copies that are interspersed with the alpha-gliadin genes.

160 in particular tissue transglutaminase (tTG), gliadin (Glia), and endomysium.

161 To clarify the conformational changes of gliadins (Glia) upon complexation with anthocyanidins (i

162 3.8 mumol N-ethylmaleimide/g protein reduces gliadin-glutenin cross-linking during pasta drying and/o

163 g 13.8 mumol glutathione/g protein increases gliadin-glutenin cross-linking during pasta processing,

164 45( * *)), gluten index (r=0.959( * *)), and gliadin/glutenin ratio (r=-0.952( * *)), while peak II i

165 r composition in terms of protein fractions (gliadins, glutenins) has been determined by means of RP-

166 microarray immunoassay with alpha/beta/gamma-gliadin, high-molecular-weight (HMW) glutenin, alpha-amy

167 Among proteins previously screened for gliadin homology, noteworthy was achaete scute homologou

168 Others were similar to DQ2-alpha-I-gliadin (i.e. PAX9; BestFit quality 46 versus 20 for ran

169 Levels of anti-gliadin IgG (but not anti-casein IgG) above the 90th per

170 High levels of anti-gliadin IgG in the maternal circulation are associated w

171 were not related to maternal levels of anti-gliadin IgG.

172 l IgA, or IgA-TTG and IgG against deamidated gliadin (IgG-DGL) could identify patients with and witho

173 n, thereafter repeatedly provoked by dietary gliadin immunodominant peptides concentrated in the prox

174 d paracellular permeability, normalized anti-gliadin immunoglobulin A in intestinal washes, and modul

175 in 14/17 patients (82%), to alpha/beta/gamma-gliadin in 82% including the three patients lacking IgE

176 al diagnostic value of alpha/beta- and gamma-gliadin in particular in omega(5) -gliadin-negative pati

177 g culture but in this instance P-T digest of gliadin in the culture medium had no significant inhibit

178 ration of proteases that can rapidly degrade gliadin in the gastric compartment has been proposed as

179 wing model for the innate immune response to gliadin in the initiation of CD.

180 oss-linking of glutenins and of inclusion of gliadin in the polymers was seen for SB6 as compared to

181 yme caricain (from papaya latex) to detoxify gliadin in whole wheat flour and develop bread suitable

182 s able to detoxify a major proportion of the gliadin in wholemeal wheat dough by allowing it to react

183 The absence of extractable alpha- and gamma-gliadins in DDGS indicated protein aggregation during th

184 utaminase 2) or the peptic-tryptic digest of gliadin (in native and deamidated forms) before T-cell c

185 We hypothesize that too much gliadin incorporation in the glutenin network during pas

186 ations were explained as resulting from less gliadin incorporation in the polymer gluten network and

187 Furthermore, binding between resveratrol and gliadin increased at higher temperatures, which was not

188 Gliadin increased zonulin release and intestinal permeab

189 Gliadin induced physical association between CXCR3 and M

190 Gliadin-induced zonulin release, increased intestinal pe

191 Gliadin induces an MyD88-dependent zonulin release that

192 We demonstrate that wheat gliadin induces significantly greater production of IL-2

193 study, we sought to test the hypothesis that gliadin initiates this response by stimulating the innat

194 The problem of gliadin insolubility was solved by mild acid treatment,

195 We aimed to establish the molecular basis of gliadin interaction with intestinal mucosa leading to in

196 Gliadin interaction with the intestinal epithelium incre

197 from patients in remission, it appears that gliadin is not directly toxic to GSE jejunal mucosa per

198 Inasmuch as this effect of gliadin is not seen in cultures of specimens taken from

199 ogen bonding, whereas their interaction with gliadins is dominated by hydrogen bonding and is relativ

200 d wheat prolamin class, given the name delta-gliadins, is the most direct ortholog of barley gamma3-h

201 with specific antibodies for gliadins, gamma-gliadins, LMW subunits and antigenic epitopes to gain a

202 and gamma-gliadin in particular in omega(5) -gliadin-negative patients in the diagnosis of WDEIA.

203 ed for IgA and IgG antibodies against native gliadin (ngli) and deamidated gliadin peptides (dpgli),

204 ectra of the wheat proteins alpha-gliadin (A-gliadin), omega-gliadin, and a 30 kDa peptide called T-A

205 , mitigating the injury of Triticum aestivum gliadin on cell viability and cytoskeleton reorganizatio

206 The effects of gliadin on crypt enterocyte proliferation and activation

207 ents were set up to assess the effects of PT gliadin on innate and adaptive immune response by using

208 In conclusion, the inhibitory effect of gliadin on intestinal epithelial cells in organ culture

209 rees C (65% relative humidity (RH)), whereas gliadin only is incorporated in the protein network at t

210 ere calculated for levels of IgG directed at gliadin or casein for nonaffective psychosis.

211 videnced by strong IgE binding to deamidated gliadins or peptides of the type QPEEPFPE.

212 subunits (HMW-GS) over low MW-GS, and omega-gliadins over alpha- and gamma-gliadins.

213 (130-30 kDa; glutenins), peak II (55-20 kDa; gliadins), peak III (28-10 kDa; low molecular weight gli

214 ), peak III (28-10 kDa; low molecular weight gliadins), peak IV and V (<10 kDa; albumins and globulin

215 st tissue transglutaminase (TG2), deamidated gliadin peptide (DGP) and endomysium (EMA).

216 intestinal permeability of undegraded alpha9-gliadin peptide 31-49 (p31-49) and 33-mer gliadin peptid

217 tudy participants, mean levels of deamidated gliadin peptide autoantibodies were 7.46 (6.92) in patie

218 odies, in disease pathogenesis, by enhancing gliadin peptide class II binding and consequent T-cell a

219 Gliadins and A-gliadin peptide P31-43, in particular, act as growth fac

220 proliferation induced by EGF, IL15, and the gliadin peptide P31-43.

221 nst tissue transglutaminase-2 and deamidated gliadin peptide), symptom frequencies, and safety.

222 ainst tissue transglutaminase and deamidated gliadin peptide, greatly facilitate diagnosis.

223 o a representative high-affinity immunogenic gliadin peptide, suggesting a common mechanism by which

224 treatment, which renders an acid-hydrolysed gliadin/peptide mixture (AHG).

225 The detection of IgG against deamidated gliadin peptides (DGP) has high specificity and better s

226 detection of serum antibodies to deamidated gliadin peptides (DGPs).

227 against native gliadin (ngli) and deamidated gliadin peptides (dpgli), as well as for IgA antibodies

228 182 Da; horseradish peroxidase, 40 kDa] and gliadin peptides [33-mer (p56-88, 3900 Da), 19-mer (p31-

229 The mechanism of translocation of gliadin peptides across the intestinal barrier has been

230 Novel deamidated gliadin peptides antibodies have better diagnostic accur

231 for the design of small molecule mimetics of gliadin peptides as selective inhibitors of tTGase.

232 progress has been made in understanding how gliadin peptides can cross the intestinal border and acc

233 residues of the TG2 epitope with deamidated gliadin peptides could be a structural basis.

234 clones specific for dominant alpha- or omega-gliadin peptides from children with celiac disease had c

235 Glutamine-rich gliadin peptides from ingested cereals, after their deam

236 lutaminase (tTGase)-catalyzed deamidation of gliadin peptides has been shown to increase their potenc

237 proliferation and innate immune response to gliadin peptides in enterocytes.

238 a9-gliadin peptide 31-49 (p31-49) and 33-mer gliadin peptides is increased in active celiac disease.

239 ases in transcellular permeability to intact gliadin peptides might be considered in treatment strate

240 ation of infiltrating MCs and the effects of gliadin peptides on intestinal MCs indicated an increase

241 One recent study using diamidated gliadin peptides showed good specificity (> or = 0.94),

242 uten epitopes, degradation of immunodominant gliadin peptides that resist intestinal proteases by exo

243 These 23 gliadin peptides were next assayed by IFN-gamma ELISPOT

244 inding to apical CD71, SIgA (with or without gliadin peptides) enters a recycling pathway and avoids

245 1 for TGA-IgG, 6 for IgG against deamidated gliadin peptides, and 1 for EMA, from 5 different manufa

246 PY, a sequence motif found in immunodominant gliadin peptides, was analyzed in detail.

247 ell to modified (deamidated) versions of the gliadin peptides, whereas HCD4DQ6 mice did not.

248 nd CD71 promote transepithelial transport of gliadin peptides.

249 not be considered markers of permeability to gliadin peptides.

250 ve and innate immune responses to undigested gliadin peptides.

251 ance the immunogenicity of the corresponding gliadin peptides.

252 gen, tissue transglutaminase, and deaminated gliadin peptides; the most frequently detected nonmitoch

253 ty 46 versus 20 for random), or DQ2-alpha-II-gliadin (PHLDA1, known in mice as the T-cell death-assoc

254 d IL-2 release at levels exceeding the wheat gliadin positive control.

255 We screened gliadin proteins (2alpha-, 2omega-, and 2gamma-gliadin)

256 pitope was found on native gamma- and omega2-gliadins (QPQQPFPQ); it was repeated several times in th

257 assay test kit was used for the analysis of gliadin residues in the baked bread.

258 l were selected for testing in a DQ2-gamma-I-gliadin-restricted T-cell system.

259 llular route, this appears to be enhanced by gliadin's stimulation of zonulin release.

260 A fourth delta-gliadin sequence occurs in the D genome of both Chinese

261 Epitopes were mapped on gamma- and omega2-gliadin sequences by Pepscan, and effect of glutamine/gl

262 The patterns of the reduced spelt gliadins showed one to three markers that were not prese

263 of intraepithelial lymphocyte cytotoxicity, gliadin-specific antibodies, and a proinflammatory gliad

264 from their serum to inhibit the binding of a gliadin-specific hmAb (1002-1E03) to a specific peptide

265 oduction and the proliferation of intestinal gliadin-specific T cell lines and clones were measured a

266 The alpha-gliadin-specific T cells from children had biases in T-c

267 heir potency for activating patient-derived, gliadin-specific T cells, suggesting that tTGase plays a

268 hough our findings emphasise the key role of gliadin-specific T cells, they suggest a complex pathoge

269 n-specific antibodies, and a proinflammatory gliadin-specific T-cell response.

270 lows the measurement of as low as 0.5 ppb of gliadin standard (0.5 ppm of gluten).

271 The use of a gliadin standard in R5 ELISA determinations causes an ov

272 The gliadins structure appeared to be progressively disorgan

273 of gliadin and/or 26 overlapping 20mer alpha-gliadin synthetic peptides to the receptor.

274 ater binding affinity for both glutenins and gliadins than lower MW PA, whereas both PA precipitated

275 eat encoding the most immunogenic peptide of gliadin that triggers the immune response of celiac dise

276 In the absence of gliadin the alkaline phosphatase activity in the biopsy

277 and styrene sulfonate complex with isolated gliadin (the toxic fraction of gluten) and prevent damag

278 In the presence of a P-T digest of gliadin, the alkaline phosphatase activity of biopsy spe

279 On ingestion of gliadin, the major protein component of wheat and other

280 iadin with Kuma030 eliminates the ability of gliadin to stimulate a T cell response.

281 sing two cut-off values (10 and 50 mg kg(-1) gliadin) to provide a semi-quantification for identifyin

282 rgets of the hmAb 1002-1E03 from a digest of gliadin treated by transglutaminase 2.

283 We carried out quantitative analysis of gliadin types by RP-HPLC to verify its correlation with

284 gens Ara h 1 and Ara h 2, and wheat allergen Gliadin using giant magnetoresistive (GMR) sensor arrays

285 iadin proteins (2alpha-, 2omega-, and 2gamma-gliadin) using bioinformatic algorithms for the presence

286 The specific IgE to omega-5 gliadin was below the detection limit in all cases.

287 By ImmunoCAP, IgE to recombinant omega(5) -gliadin was detectable in 14/17 patients (82%), to alpha

288 e 96-well-based ELISA developed in-house for Gliadin was found to have a LOD of 40 ng/mL.

289 As a result of its use, a lower level of gliadin was incorporated into the gluten polymer and dou

290 0 the original molecular weight while native gliadin was nearly eliminated from the waxy flour follow

291 thermal dependent behavior of glutenins and gliadins was analyzed through intrinsic fluorescence par

292 f DME silencing on accumulation of LMWgs and gliadins was studied using 20 transformants expressing h

293 d assays developed for Ara h 1, Ara h 2, and Gliadin were 7.0 ng/mL, 0.2 ng/mL, and 1.5 ng/mL, respec

294 A series of overlapping peptides spanning gliadin were synthesized.

295 epitopes of alpha-/beta-, gamma- and omega5-gliadins were highlighted after performing molecular dyn

296 As glutenin-bound omega-gliadins were present in wheat and absent in spelt, this

297 A culture medium, in which gliadins were the sole source of nitrogen, was developed

298 eacted to many of the individual peptides of gliadin, while the HCD4/DQ6 mice were relatively unrespo

299 Indeed, treatment of gliadin with Kuma030 eliminates the ability of gliadin t

300 There, the interaction of gliadin with macrophages elicits a MyD88-dependent proin